CN109696403A - A kind of sample room for immersion micro-imaging - Google Patents
A kind of sample room for immersion micro-imaging Download PDFInfo
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- CN109696403A CN109696403A CN201710994401.7A CN201710994401A CN109696403A CN 109696403 A CN109696403 A CN 109696403A CN 201710994401 A CN201710994401 A CN 201710994401A CN 109696403 A CN109696403 A CN 109696403A
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- pedestal
- gland
- window assembly
- sample room
- pin hole
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0378—Shapes
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The present invention relates to micro-imaging technique fields, and the sample room of immersion micro-imaging is used for more particularly to one of optical tomography and whole-field optically chromatography imaging technique.Including pedestal and gland, the top of the pedestal is equipped with the boss for carrying sample, the window assembly for pushing down sample is arranged on the boss, the bottom of the window assembly is connect by elastic mechanism with pedestal, and window assembly is driven to float up and down adjustment by elastic mechanism, the gland pushes down the top of window assembly, and is capable of the installation of lift adjustment on the base, and gland lift adjustment depresses the height of window assembly.The beneficial effects of the present invention are: it is simple and compact for structure, the height of stable adjustment window assembly is cooperated by gland, elastic mechanism and pedestal, so that window assembly, which floats, compresses sample, adjusting is simple to operate, versatile.
Description
Technical field
The present invention relates to micro-imaging technique fields, more particularly to optical tomography and whole-field optically tomography skill
One of art is used for the sample room of immersion micro-imaging.
Background technique
The optical system of optical microscopy mainly includes four object lens, eyepiece, reflective mirror and condenser components, wherein object lens
It is divided into dry type object lens and immersion objective, and using the microscope imaging process of immersion objective to call immersion micro-imaging.
Object can be amplified 1600 times by present optical microscopy, and the least limit of resolution can reach 1/2 wavelength, in scientific research and work
The fields such as industry production have very extensive application prospect.
Microscope usually needs to carry out in use the storage or movement of sample by sample room, and current
In microscope example room, mostly there are Design of Mechanical Structure more complicated, components using more, volume is larger, assembly not
Just with maintenance difficult the shortcomings that.
Summary of the invention
In view of the foregoing deficiencies of prior art, the purpose of the present invention is to provide one kind to be used for immersion micro-imaging
Sample room, for solve in the prior art sample room structure disperses, adjust the problems such as inconvenient, poor universality.
In order to achieve the above objects and other related objects, the present invention provides a kind of sample for immersion micro-imaging
Room, including pedestal and gland, the top of the pedestal are equipped with the boss for carrying sample, are arranged on the boss for pressing
The bottom of the firmly window assembly of sample, the window assembly is connect by elastic mechanism with pedestal, and is driven by elastic mechanism
Window assembly floats up and down adjustment, and the gland pushes down the top of window assembly, and is capable of the installation of lift adjustment on the base,
The height of gland lift adjustment pressure window assembly.
The beneficial effects of the present invention are: it is simple and compact for structure, stable adjustment is cooperated by gland, elastic mechanism and pedestal
The height of window assembly, so that window assembly, which floats, compresses sample, adjusting is simple to operate, versatile.
Further, the window assembly includes sleeve, and one end of the sleeve is equipped with the limiting section extended inwardly, described
Installation sheet glass and pressing ring in sleeve, and sheet glass, between limiting section and pressing ring, the pressing ring is connect with bush whorl, is turned
Moving sleeve makes pressing ring mobile and limiting section cooperates pressed glass piece, and simple and compact for structure, assembly manipulation is simple and convenient.
Further, the sleeve is equipped with the first pin hole far from one end of limiting section, and the pedestal is equipped with and the first pin hole
Corresponding second pin hole, the both ends of the elastic mechanism are separately mounted in the first pin hole and the second pin hole.
Further, the elastic mechanism includes pin and spring, and the upper end of the pin is mounted in the first pin hole, described
The lower end of pin is mounted in the second pin hole, and the spring is set on pin, and is floated upwards for sleeve and provided elastic force.
Further, the quantity at least two of first pin hole, and the quantity phase of first pin hole and the second pin hole
Together.
Further, the quantity of first pin hole and the second pin hole is three.
Further, the gland is connected with base thread.
Beneficial effect using above-mentioned further scheme is: gland is connected with base thread, and operation is adjusted simply, and is had
Conducive to the leakproofness guaranteed inside sample room, assembly elastic mechanism is not only convenient for by the first pin hole of setting and the second pin hole, together
When be also able to achieve constraint to window assembly rotary freedom, by gland and elastic mechanism cooperation to the position of window assembly into
Row accurate adjustment improves degree of regulation.
It further, further include adjustment base, the adjustment base includes knob and the threaded rod that is arranged on knob, described
The bottom of pedestal is equipped with the threaded hole with shaft, and turning knob makes pedestal lift adjustment, convenient for fast implementing pedestal
Lift adjustment, adjust it is easy to operate.
Further, the adjustment base and gland are separately mounted to the both ends of pedestal, and are formed and opposite with pedestal can be turned
Dynamic symmetrical structure, it is simple and compact for structure.
Further, the top that the gland is located at boss is equipped with observation panel, is equipped with transparent panel at observation panel, is guaranteeing sample
Convenient for the sample on observation boss while product chamber interior sealing performance.
Detailed description of the invention
Fig. 1 is shown as the structural schematic diagram of the sample room for immersion micro-imaging of the embodiment of the present invention；
Fig. 2 is shown as the cross-sectional view of the sample room for immersion micro-imaging of the embodiment of the present invention；
Fig. 3 is shown as the structural schematic diagram of the gland of the sample room for immersion micro-imaging of the embodiment of the present invention；
Fig. 4 is shown as the structural representation at the top of the sleeve of the sample room for immersion micro-imaging of the embodiment of the present invention
Figure；
Fig. 5 is shown as the structural representation of the sleeve bottom of the sample room for immersion micro-imaging of the embodiment of the present invention
Figure；
Fig. 6 is shown as the structural representation of the sheet glass of the sample room for immersion micro-imaging of the embodiment of the present invention
Figure；
Fig. 7 is shown as the structural schematic diagram of the pressing ring of the sample room for immersion micro-imaging of the embodiment of the present invention；
Fig. 8 is shown as the structural representation at the top of the pedestal of the sample room for immersion micro-imaging of the embodiment of the present invention
Figure；
Fig. 9 is shown as the structural representation of the base bottom of the sample room for immersion micro-imaging of the embodiment of the present invention
Figure；
The structure that Figure 10 is shown as the adjustment base of the sample room for immersion micro-imaging of the embodiment of the present invention is shown
It is intended to.
Piece mark explanation
1 gland；
11 fine threads；
12 limiting sections；
13 outsides cylindrical surface；
14 observation panels；
2 sleeves；
21 limiting sections；
22 internal screw threads；
23 first pin holes；
24 outsides cylindrical surface；
3 sheet glass；
4 pressing rings；
5 pins；
6 springs；
7 pedestals；
71 second pin holes；
72 boss；
73 external screw threads；
74 outsides cylindrical surface；
75 threaded holes；
8 adjustment bases；
81 knobs；
82 threaded rods.
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification
Other advantages and efficacy of the present invention can be easily understood for disclosed content.The present invention can also pass through in addition different specific realities
The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints and application, without departing from
Various modifications or alterations are carried out under spirit of the invention.
It should be noted that cited such as "upper", "lower", "left", "right", " centre " and " one " in this specification
Term is merely convenient to being illustrated for narration, rather than to limit the scope of the invention, the change of relativeness or tune
It is whole, under the content of no substantial changes in technology, when being also considered as the enforceable scope of the present invention.
Before the embodiment of the present invention is described in detail, first application environment of the invention is described.The present invention
Technology mainly be applied to micro-imaging technique field, be especially applied to optical tomography (OCT) and whole audience light
It learns chromatography imaging technique (FFOCT), for example is used for high magnification microscope, scanning confocal microscope, bis- (more) photon microscopes, light
Learn the sample room of the immersion micro-imagings of imagings such as chromatography microscope.
As depicted in figs. 1 and 2, the sample room for immersion micro-imaging of the embodiment of the present invention, including pedestal 7, tune
Pedestal 8 and gland 1 are saved, adjustment base 8 and gland 1 are separately mounted to the both ends of pedestal 7, and are formed and opposite with pedestal 7 can be turned
Dynamic symmetrical structure.The cylindrical structure in sample room completed is assembled, gland 1 and adjustment base 8 are symmetrical arranged.
As shown in Figures 1 to 10, the top of pedestal 7 is equipped with the boss 72 for carrying sample, is arranged on boss 72 useful
It is connect by elastic mechanism with pedestal 7 in the bottom of the window assembly for pushing down sample, window assembly, and is driven by elastic mechanism
Window assembly floats up and down adjustment, so that window assembly is able to achieve the fixation to tissue sample.Gland 1 pushes down the top of window assembly
Portion, gland are capable of being mounted on pedestal 7 of lift adjustment, and 1 lift adjustment of gland depresses the height of window assembly, wherein pedestal 7
For stepped cylinder structure, pedestal 7 is equipped with external screw thread 73, and the top of gland 1 is equipped with the limiting section 12 extended inwardly, limit
Portion 12 pushes down window assembly, and 1 inside cylindrical face of gland is equipped with fine thread 11, and gland 1 and pedestal 7 are threadedly coupled, rotation pressure
1 oscilaltion of lid, and elastic mechanism is cooperated just to realize the height adjustment of window assembly.The outside cylindrical surface 13 of gland 1 and base
It is equipped with and embroiders on the outside cylindrical surface 74 of seat 7, when rotation process, increase the frictional force contacted in operation with finger.
The top that gland 1 is located at boss 72 is equipped with observation panel 14, is equipped with transparent panel at observation panel 14, is being convenient for by the way that transparent panel is arranged
The leakproofness inside sample room is also ensured when observing the sample on boss 72.
As shown in Figures 1 to 10, window assembly includes sleeve 2, and one end of sleeve 2 is equipped with the limiting section extended inwardly
21, sleeve 2 is interior to install sheet glass 3 and pressing ring 4, and between limiting section 21 and pressing ring 4, the outer wall of pressing ring 4 is equipped with outer sheet glass 3
The inner wall of screw thread, sleeve 2 is equipped with internal screw thread 22, and pressing ring 4 is threadedly coupled with sleeve 2, and when window assembly assembles, rotational sleeve 2 makes
It obtains the movement of pressing ring 4 and cooperates pressed glass piece 3 with limiting section 21, complete the assembly of window assembly.Sheet glass 3 can using commonly at
The window glass of picture, also can be used glass slide, thickness is selected according to microscope.Between sleeve 2 and pressing ring 4
Interaction force is little, can be fabricated using aluminum alloy materials, advantageously reduce weight and cost.The outside circle of sleeve 2
Cylinder 24 is equipped with and embroiders, and increases the frictional force contacted in operation with finger.Sleeve 2 is equipped with far from one end of limiting section 21
First pin hole 23, pedestal 7 are equipped with second pin hole 71 corresponding with the first pin hole 23, and the both ends of elastic mechanism are separately mounted to the
In one pin hole 23 and the second pin hole 71, the aperture of the second pin hole 71 is greater than the aperture of the first pin hole 23.Elastic mechanism includes pin 5
With spring 6, the diameter of pin 5 is less than the internal diameter of spring 6, and the upper end of pin 5 is mounted in the first pin hole 23, and with the first pin hole
23 are fitted close；The lower end of pin 5 is mounted in the second pin hole 71, and the lower end of spring 6 is mounted in the second pin hole 71, and with
Two pin holes, 71 inner wall is fitted close, and spring 6 is set on pin 5, and the second pin hole 71 is stretched out in the upper end of spring 6, withstands sleeve 2,
It floats upwards for sleeve 2 and elastic force is provided, when 1 push-window component of gland, spring 6 generates damping to window assembly.First
The quantity of pin hole 23 is at least one, and the first pin hole 23 is identical with the quantity of the second pin hole 71, in the present embodiment, the first pin
The quantity of hole 23 and the second pin hole 71 is three, can not only by the cooperation of the first pin hole 23, the second pin hole 71 and elastic mechanism
It is enough that the fixed sample of adjustment that floats is carried out to the position of window assembly, moreover it is possible to limit the rotary freedom of sleeve.
As shown in Figures 1 to 10, adjustment base 8 includes knob 81 and the threaded rod 82 being arranged on knob 81, pedestal 7
Bottom is equipped with the threaded hole 75 cooperated with threaded rod 82, and turning knob 81 makes 7 lift adjustment height of pedestal, to realize sample
The height adjustment of room.In use, adjustment base 8 can be used cooperatively with Two-dimensional Position moving stage, facilitate sample room mobile.
Sample room of the invention has the advantage that
1) sample fix stably.Interaction between the elastic force of the pressure and spring that are generated by gland, it is accurate to control
The position of sheet glass and the stress of sample, so as to guarantee the stabilization of sample；The rotation of window assembly is limited using pin
Freedom degree ensure that sample in fixation procedure, i.e., during the pushing of gland, with sheet glass contact position phase does not occur for sample
To displacement.
2) compact-sized, it is easy to carry.The sample room includes that sample is fixed and whole height adjusts two functions simultaneously, no
It needing additionally to increase height adjustment mechanism, is threadedly engaged by itself and height adjustment can be realized, small volume is easy to carry,
Also ensure sample room inner sealing performance.
3) structure is simple, high reliablity.The sample room is compared with conventional sample chamber, reduces the quantity of part, no fastening
The fasteners such as screw or bolt；Sample room height is adjusted using Mechanical Method, reduces the variable of adjustment process.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (10)
1. a kind of sample room for immersion micro-imaging, it is characterised in that: including pedestal and gland, the top of the pedestal
Equipped with the boss for carrying sample, the window assembly for pushing down sample is arranged on the boss, the window assembly
Bottom is connect by elastic mechanism with pedestal, and is floated up and down adjustment by elastic mechanism drive window assembly, the gland pressure
The firmly top of window assembly, and it is capable of the installation of lift adjustment on the base, gland lift adjustment depresses the height of window assembly.
2. a kind of sample room for immersion micro-imaging according to claim 1, it is characterised in that: the group of windows
Part includes sleeve, and one end of the sleeve is equipped with the limiting section extended inwardly, and sheet glass and pressing ring are installed in the sleeve, and
Between limiting section and pressing ring, the pressing ring is connect sheet glass with bush whorl, and rotational sleeve makes pressing ring mobile and limit
Portion cooperates pressed glass piece.
3. a kind of sample room for immersion micro-imaging according to claim 2, it is characterised in that: the sleeve is remote
One end from limiting section is equipped with the first pin hole, and the pedestal is equipped with the second pin hole corresponding with the first pin hole, the elasticity machine
The both ends of structure are separately mounted in the first pin hole and the second pin hole.
4. a kind of sample room for immersion micro-imaging according to claim 3, it is characterised in that: the elasticity machine
Structure includes pin and spring, and the upper end of the pin is mounted in the first pin hole, and the lower end of the pin is mounted on the second pin hole
In, the spring is set on pin, and is floated upwards for sleeve and provided elastic force.
5. a kind of sample room for immersion micro-imaging according to claim 3, it is characterised in that: first pin
The quantity in hole is at least one, and the quantity of first pin hole and the second pin hole is identical.
6. a kind of sample room for immersion micro-imaging according to claim 5, it is characterised in that: first pin
The quantity of hole and the second pin hole is three.
7. a kind of sample room for immersion micro-imaging according to claim 1, it is characterised in that: the gland and
Base thread connection.
8. a kind of sample room for immersion micro-imaging according to claim 1, it is characterised in that: further include adjusting
Pedestal, the adjustment base include knob and the threaded rod that is arranged on knob, and the bottom of the pedestal is equipped with matches with threaded rod
The threaded hole of conjunction, turning knob make pedestal lift adjustment.
9. a kind of sample room for immersion micro-imaging according to claim 8, it is characterised in that: the adjusting bottom
Seat and gland are separately mounted to the both ends of pedestal, and form the symmetrical structure that can be relatively rotated with pedestal.
10. a kind of sample room for immersion micro-imaging according to claim 1, it is characterised in that: the gland
It is equipped with observation panel positioned at the top of boss, transparent panel is installed at observation panel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710994401.7A CN109696403B (en) | 2017-10-23 | 2017-10-23 | Sample chamber for immersed microscopic imaging |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710994401.7A CN109696403B (en) | 2017-10-23 | 2017-10-23 | Sample chamber for immersed microscopic imaging |
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| CN109696403A true CN109696403A (en) | 2019-04-30 |
| CN109696403B CN109696403B (en) | 2021-08-13 |
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| CN201710994401.7A Active CN109696403B (en) | 2017-10-23 | 2017-10-23 | Sample chamber for immersed microscopic imaging |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111419181A (en) * | 2020-03-31 | 2020-07-17 | 浙江大学 | Device for long-time living body imaging of small animal and using method thereof |
| WO2021147198A1 (en) * | 2020-01-21 | 2021-07-29 | 广东湛江海洋医药研究院 | Multi-photon microscope transparent specimen adapter and imaging method thereof |
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