CN109694828A - The comprehensive processing technique of threonine mother liquor - Google Patents
The comprehensive processing technique of threonine mother liquor Download PDFInfo
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- CN109694828A CN109694828A CN201811581412.3A CN201811581412A CN109694828A CN 109694828 A CN109694828 A CN 109694828A CN 201811581412 A CN201811581412 A CN 201811581412A CN 109694828 A CN109694828 A CN 109694828A
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- 239000012452 mother liquor Substances 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 29
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 239000004473 Threonine Substances 0.000 title claims abstract description 26
- 238000012545 processing Methods 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 25
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 22
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 22
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 22
- 241000192534 Dolichospermum flos-aquae Species 0.000 claims abstract description 16
- 235000015097 nutrients Nutrition 0.000 claims abstract description 11
- 239000002054 inoculum Substances 0.000 claims description 23
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 241000195493 Cryptophyta Species 0.000 claims description 11
- 244000005700 microbiome Species 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- 239000002609 medium Substances 0.000 claims description 7
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- 239000004745 nonwoven fabric Substances 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 4
- 230000003698 anagen phase Effects 0.000 claims description 4
- 235000013351 cheese Nutrition 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 238000003973 irrigation Methods 0.000 claims description 4
- 230000002262 irrigation Effects 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 241000186660 Lactobacillus Species 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
- 239000000654 additive Substances 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 241000222122 Candida albicans Species 0.000 claims 1
- 206010007134 Candida infections Diseases 0.000 claims 1
- 201000003984 candidiasis Diseases 0.000 claims 1
- 238000011081 inoculation Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000012092 media component Substances 0.000 abstract description 3
- 239000002351 wastewater Substances 0.000 description 12
- 150000001413 amino acids Chemical class 0.000 description 11
- 239000003344 environmental pollutant Substances 0.000 description 10
- 231100000719 pollutant Toxicity 0.000 description 10
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 7
- 239000002699 waste material Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 244000249201 Scenedesmus obliquus Species 0.000 description 2
- 235000007122 Scenedesmus obliquus Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000003546 flue gas Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000005453 pelletization Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000003911 water pollution Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010067171 Regurgitation Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002921 fermentation waste Substances 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 239000003621 irrigation water Substances 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Biotechnology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
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- Botany (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention belongs to field of biotechnology, disclose the comprehensive processing technique of threonine mother liquor comprising following steps: step 1) prepares the zymocyte liquid of candida utili bacterium and Lactobacillus casei, and step 2 prepares Anabaena Flos-aquae liquid, step 3) mother liquid disposal.Present invention process does not need additionally to add nutrient media components, simple process and low cost, is suitble to the extensive mother liquor of processing.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to the comprehensive processing technique of threonine mother liquor.
Background technique
China is amino acids production and consumption big country, and often discharge largely contains in the industry productions such as chemical industry, pharmacy, food
The waste liquor of amino acid shows as high COD, high NH containing there are many amino acid, residual sugar, fermentation intermediate product, ammonium salt etc.3- N's
Feature, and in acidity, it after being discharged into water body, would seriously pollute the environment, difficulty of governance is larger.To solve waste water pollution problem, together
When waste water resource is efficiently used, in traditional production technology, first mycoprotein in amino acid waste liquor is extracted
It is sold to be made into feed, the waste liquor after extracting albumen is concentrated to prepare fertilizer regurgitation feeding agriculture using spray granulation.
Amino acid fermentation waste liquid slurry-spraying pelletizing technique is that high-concentration waste water is effectively treated in amino acids production enterprise, realizes circulation
Economy increases the effective way of business economic income, but during fermented waste fluid slurry-spraying pelletizing, produces containing large quantity of moisture
With the pollution flue gas of organic matter, environment " secondary pollution " is easily caused.It is normal that there is the flue gas serious peculiar smell to influence the masses around
Life, become local government and urgently solve the problems, such as, be long-standing problem fermentation industry development significant technology issues.
Some researches show that can use amino acids industry waste water and produce feeding mycoprotein, such as Chinese patent technology " benefit
With the method for the inferior Dbaly yeast of the high feeding Chinese of saliferous amino acid wastewater fermenting and producing " to disclose a kind of recycling amino acid useless
The method that water carries out fermenting and producing mycoprotein, this method are prepared for culture medium using waste water, and nutrient media components are high saliferous ammonia
Base acid sewage: 125g/L-330g/L, carbon source: 30-100g/L, potassium dihydrogen phosphate (KH2PO4): 5-15g/L, yeast powder: 0-10g/
L, magnesium sulfate (MgSO4): 3-9g/L, calcium carbonate (CaCO3): 0.75-2g/L, ferrous sulfate (FeSO4): 1.5-4g/L, culture medium
PH is 3.5-7.5, and waste water has been effectively treated in this method, and is prepared for mycoprotein；But there is also some defects for this method:
The defects of culture medium raw material higher cost, limited waste water usage amount.
Since waste liquor contains multiple nutritional components, during storage pool memory is put, a large amount of microbial floras can be bred,
With the accumulation of storage pool runing time, the dominant microflora for being adapted to the low pH of waste liquor, high salt adverse circumstances is formd in pond.
Research and development centre, Fu Feng group had once carried out case study (Li Haiyan, the equal " dominant bacteria in gourmet powder waste water of Xu Guohua to this project
The research of separation screening and growth characteristics ", science and technology of fermenting communication, the 4th phase in 2008), the results showed that it is raw in gourmet powder waste water
A large amount of microorganism not of the same race is had, under certain ecological condition, the growth of various microorganisms is in equilibrium state；Wherein
Dominant growth bacterium remains dominant growth in waste water, and the ratio of microbial count is maximum in water body shared by increment, by grinding
Study carefully the external condition for changing dominant bacteria, improves its growth activity, will be greatly improved to the degradation effect of Wastewater Pollutant.
As it can be seen that amino acid waste liquid is suitble to the breeding of microorganism, it can be with pollutant in decomposition nut liquid；We can be specific by adding
The microorganism of mother liquor environment is adapted to, to decompose the pollutant in span amino acid mother liquor, to achieve the purpose that integrated treatment mother liquor.
Patented technology " CN107267427A, a kind of threonine mother liquor recycling method " before applicant utilizes withered grass
Bacillus, bacillus licheniformis and scenedesmus obliquus handle mother liquor, and effect is preferable, but need to withered grass gemma
Bacillus, bacillus licheniformis and scenedesmus obliquus are tamed, and need additional addition nutrient media components, complex process, at
This raising is unfavorable for handling extensive mother liquor.
Summary of the invention
In order to overcome the existing technological deficiency of the prior art further to be improved, the present invention provides threonine mothers
The comprehensive processing technique of liquid.
For achieving the above object, the invention adopts the following technical scheme:
The comprehensive processing technique of threonine mother liquor comprising following steps: step 1) prepares candida utili bacterium and cheese cream
The zymocyte liquid of bacillus, step 2 prepare Anabaena Flos-aquae liquid, step 3) mother liquid disposal.
Further,
Step 1) prepares the zymocyte liquid of candida utili bacterium and Lactobacillus casei: taking appropriate threonine mother liquor, 100 DEG C are boiled
Then 5min adds ammonium chloride, stir evenly, then is successively inoculated with candida utili bacterium seed liquor and Lactobacillus casei seed
Liquid, temperature are 28-32 DEG C, with 200rpm shake culture 12h, are then allowed to stand culture for 24 hours, obtain candida utili bacterium and cheese
The zymocyte liquid of lactobacillus；
Step 2 prepares Anabaena Flos-aquae liquid: the Anabaena Flos-aquae in logarithmic growth phase is inoculated into according to 10% inoculum concentration
It is cultivated in BG11 culture medium, the condition of culture are as follows: intensity of illumination 5000lux, 25 DEG C of cultures, Light To Dark Ratio 12:12, ventilation
Rate is 0.2vvm；48h is cultivated, algae solution is collected；
Step 3) mother liquid disposal: threonine mother liquor is discharged in reaction tank, is then laid with non-woven fabrics as attachment carrier, then will hair
Yeast-like fungi liquid is inoculated into reaction tank, and the processing time is 2-3d, and then algae solution is inoculated into reaction tank, continues with 6-7d, plate
Microorganism is collected by filtration in frame, is then discharged out liquid, is used for field irrigation.
Preferably,
In the step 1), the additive amount of ammonium chloride accounts for the 0.5-1% parts by weight of threonine mother liquor.
Preferably,
In the step 1), the inoculum concentration of the candida utili bacterium seed liquor and Lactobacillus casei seed liquor is 5-10%.
Preferably,
In the step 3), the inoculum concentration of the zymocyte liquid is 1-2%.
Preferably,
In the step 3), the inoculum concentration of the algae solution is 0.5-1%.
Preferably,
In the step 1), the candida utili bacterium seed liquor the preparation method comprises the following steps: candida utili bacterium is inoculated into
It is cultivated in YPD fluid nutrient medium, obtains candida utili bacterium seed liquor.
Preferably,
In the step 1), the Lactobacillus casei seed liquor is trained the preparation method comprises the following steps: Lactobacillus casei is inoculated into MRS liquid
It supports and is cultivated in base, obtain Lactobacillus casei seed liquor.
Compared with prior art, the beneficial effect that the present invention obtains mainly includes but is not limited to the following aspects:
Since oxygen content is lower in mother liquor, the present invention is inoculated with candida utili bacterium and Lactobacillus casei first, and two kinds of bacterial strains can
With mutual symbiosis, under anaerobic, macromolecular organic matter is decomposed, generate small organic molecule, inhibit miscellaneous bacteria, the later period passes through again
Anabaena Flos-aquae is degraded the organic nutrient substances such as nitrogen, phosphorus in mother liquor in the way of small molecule organic matter, also using too
Sunlight carries out photosynthesis to guarantee the growth and breeding of itself, absorbing carbon dioxide, and generates oxygen, moreover it is possible to promote bacterial strain raw
It is long, entire method simple possible, low in cost, high treating effect；Non-woven fabrics large specific surface area, it is not easy to it is corroded, microorganism
It can adhere to form biomembrane, biofilm effect is good, and treatment effeciency is high.
Figure of description
Fig. 1: influence of each factor to COD in mother liquor and ammonia nitrogen removal frank；
Fig. 2: influence of the inoculum concentration of Anabaena Flos-aquae to major pollutants COD and ammonia nitrogen removal frank.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, having below in conjunction with the application
Body embodiment more clearly and completely describes the present invention, it is clear that described embodiment is only the application one
Divide embodiment, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making
Every other embodiment obtained, should fall within the scope of the present invention under the premise of creative work.
Embodiment 1
The comprehensive processing technique of threonine mother liquor comprising following steps:
Candida utili bacterium is inoculated into YPD fluid nutrient medium and is cultivated, candida utili bacterium seed liquor, bacterium are obtained
Bulk concentration is 1 × 109CFU/ml；
Lactobacillus casei is inoculated into MRS fluid nutrient medium and is cultivated, Lactobacillus casei seed liquor, cell concentration control are obtained
System is 3 × 109CFU/ml；
Appropriate threonine mother liquor is taken, 100 DEG C are boiled 5min, and then addition accounts for the ammonium chloride of 0.6% parts by weight of threonine mother liquor, are stirred
It mixes uniformly, is then successively inoculated with candida utili bacterium seed liquor and Lactobacillus casei seed liquor, inoculum concentration are 10%, temperature
It is 30 DEG C, with 200rpm shake culture 12h, is then allowed to stand culture for 24 hours, obtains zymocyte liquid；
It will be in the Anabaena Flos-aquae of logarithmic growth phase, is inoculated into BG11 culture medium and is cultivated according to 10% inoculum concentration,
Inoculum density is 1 × 105A/ml, the condition of culture are as follows: intensity of illumination 5000lux, 25 DEG C of cultures, Light To Dark Ratio 12:12 lead to
Gas velocity rate is 0.2vvm；48h is cultivated, algae solution is collected；
Threonine mother liquor is discharged in reaction tank, non-woven fabrics is then laid with as attachment carrier, will be sent out according still further to 2% inoculum concentration
Yeast-like fungi liquid is linked into reaction tank, and the processing time is 2d, and then algae solution is linked into reaction tank according to 1% inoculum concentration, is continued
6d is handled, plate-frame filtering collects microorganism, is then discharged out liquid, is used for field irrigation.
Embodiment 2
The comprehensive processing technique of threonine mother liquor comprising following steps:
Candida utili bacterium is inoculated into YPD fluid nutrient medium and is cultivated, candida utili bacterium seed liquor, bacterium are obtained
Bulk concentration is 2 × 109CFU/ml；
Lactobacillus casei is inoculated into MRS fluid nutrient medium and is cultivated, Lactobacillus casei seed liquor, cell concentration control are obtained
System is 5 × 109CFU/ml；
Appropriate threonine mother liquor is taken, 100 DEG C are boiled 5min, and then addition accounts for the ammonium chloride of 0.5% parts by weight of threonine mother liquor, are stirred
It mixes uniformly, is then successively inoculated with candida utili bacterium seed liquor and Lactobacillus casei seed liquor, inoculum concentration are 6%, temperature is
It 28 DEG C, with 200rpm shake culture 12h, is then allowed to stand culture for 24 hours, obtains zymocyte liquid；
It will be in the Anabaena Flos-aquae of logarithmic growth phase, is inoculated into BG11 culture medium and is cultivated according to 10% inoculum concentration,
Inoculum density is 1 × 105A/ml, the condition of culture are as follows: intensity of illumination 5000lux, 25 DEG C of cultures, Light To Dark Ratio 12:12 lead to
Gas velocity rate is 0.2vvm；48h is cultivated, algae solution is collected；
Threonine mother liquor is discharged in reaction tank, non-woven fabrics is then laid with as attachment carrier, will be sent out according still further to 1% inoculum concentration
Yeast-like fungi liquid is linked into reaction tank, and the processing time is 3d, and then algae solution is linked into reaction tank according to 0.5% inoculum concentration, after
Continuous processing 7d, plate-frame filtering collect microorganism, are then discharged out liquid, are used for field irrigation.
Embodiment 3
The threonine mother liquor that the present invention uses is the raffinate after extracting threonine in fermentation liquid, randomly selects 2018 Nian Sanji
Certain a batch of threonine mother liquor of degree is tested, before detecting threonine mother liquid disposal, treated each pollutant index, tool
Body is shown in Table 1:
Table 1
| Index | Before processing | After processing (1 technique of embodiment) |
| COD(mg/L) | 3218.5 | 53.1 |
| Ammonia nitrogen (mg/L) | 416.2 | 12.8 |
| SS(mg/L) | 157.3 | 7.9 |
| P(mg/L) | 113.8 | 5.7 |
| Crude protein (g/L) | 29.6 | 4.1 |
As shown in Table 1, after treatment, the pollutant in mother liquor and the crude protein content range of decrease are obvious, and water quality completely can be with
For field irrigation water source, energy-saving and emission-reduction reduce the environmental protection pressure of enterprise.
Embodiment 4
Using major pollutants COD and ammonia nitrogen as Testing index, influence of each factor to mother liquid disposal is verified.
Different control groups is set, wherein control 1: does not add candida utili bacterium, remaining is the same as embodiment 1；Control 2:
Lactobacillus casei is not added, remaining is the same as embodiment 1；Control 3: Anabaena Flos-aquae is not added, remaining is the same as embodiment 1；Control 4: three
Kind microorganism is accessed simultaneously, remaining is the same as embodiment 1.Experimental group is embodiment.As shown in Figure 1, compared with control 1-4, experiment
Group is broken down into small molecule to part larger molecular organics, then using candida utili bacterium and Lactobacillus casei is first accessed
The mode of Anabaena Flos-aquae is accessed, Anabaena Flos-aquae proliferative speed is improved, can be improved the removal rate of major pollutants in mother liquor,
Effect is better than control 1-4.
Embodiment 5
Influence of the inoculum concentration of Anabaena Flos-aquae to major pollutants COD and ammonia nitrogen removal frank.
The inoculum concentration that inoculum concentration is respectively 0,0.25,0.5,1.0,2.0,4.0 (%) six different gradients is arranged to be examined
It surveys, as shown in Fig. 2, COD and ammonia nitrogen removal frank improve rapidly with the increase of inoculum concentration, when increasing to 1%, reaches peak value,
Then as the increase of inoculum concentration, COD and ammonia nitrogen removal frank decline, it may be possible to because of Anabaena Flos-aquae excessively fast passage, cause algae
Class Mortality and water pollution are unfavorable for the removal of pollutant instead.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. the comprehensive processing technique of threonine mother liquor comprising following steps: step 1) prepares candida utili bacterium and cheese
The zymocyte liquid of lactobacillus, step 2 prepare Anabaena Flos-aquae liquid, step 3) mother liquid disposal.
2. comprehensive processing technique according to claim 1, which is characterized in that
Step 1) prepares the zymocyte liquid of candida utili bacterium and Lactobacillus casei: taking appropriate threonine mother liquor, 100 DEG C are boiled
Then 5min adds ammonium chloride, stir evenly, then is successively inoculated with candida utili bacterium seed liquor and Lactobacillus casei seed
Liquid, temperature are 28-32 DEG C, with 200rpm shake culture 12h, are then allowed to stand culture for 24 hours, obtain candida utili bacterium and cheese
The zymocyte liquid of lactobacillus；
Step 2 prepares Anabaena Flos-aquae liquid: the Anabaena Flos-aquae in logarithmic growth phase is inoculated into according to 10% inoculum concentration
It is cultivated in BG11 culture medium, the condition of culture are as follows: intensity of illumination 5000lux, 25 DEG C of cultures, Light To Dark Ratio 12:12, ventilation
Rate is 0.2vvm；48h is cultivated, algae solution is collected；
Step 3) mother liquid disposal: threonine mother liquor is discharged in reaction tank, is then laid with non-woven fabrics as attachment carrier, then will hair
Yeast-like fungi liquid is inoculated into reaction tank, and the processing time is 2-3d, and then algae solution is inoculated into reaction tank, continues with 6-7d, plate
Microorganism is collected by filtration in frame, and liquid is finally discharged, and is used for field irrigation.
3. comprehensive processing technique according to claim 2, which is characterized in that in the step 1), the additive amount of ammonium chloride
Account for the 0.5-1% parts by weight of threonine mother liquor.
4. comprehensive processing technique according to claim 2, which is characterized in that in the step 1), the Candida utilis ferment
The inoculum concentration of female bacterium seed liquor and Lactobacillus casei seed liquor is 5-10%.
5. comprehensive processing technique according to claim 2, which is characterized in that in the step 3), the zymocyte liquid
Inoculum concentration is 1-2%.
6. comprehensive processing technique according to claim 2, which is characterized in that in the step 3), the inoculation of the algae solution
Amount is 0.5-1%.
7. comprehensive processing technique according to claim 2, which is characterized in that in the step 1), the Candida utilis ferment
Female bacterium seed liquor is cultivated the preparation method comprises the following steps: candida utili bacterium is inoculated into YPD fluid nutrient medium, is obtained and is produced protein
Candidiasis seed liquid.
8. comprehensive processing technique according to claim 2, which is characterized in that in the step 1), the Lactobacillus casei
Seed liquor is cultivated the preparation method comprises the following steps: Lactobacillus casei is inoculated into MRS fluid nutrient medium, obtains Lactobacillus casei kind
Sub- liquid.
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