CN109691595A - A kind of Chinese herbal feed additive improving infection salmonella growth performance of chicks - Google Patents
A kind of Chinese herbal feed additive improving infection salmonella growth performance of chicks Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
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Abstract
The present invention relates to a kind of Chinese herbal feed additives for improving infection salmonella growth performance of chicks, belong to chicken feed addictive field, the Chinese herbal feed additive of the improvement infection salmonella growth performance of chicks includes square foundation and prescription, square foundation comprises the following components in parts by weight: 10-15 parts of Radix Angelicae Sinensis, 10-15 parts of fructus lycii, 10-15 parts of Chinese yam, 1-6 parts of Radix Salviae Miltiorrhizae, 10-15 parts of Schisandra chinensis, 2-8 parts of corydalis tuber, 3-9 parts of frutus cnidii, 2-8 parts of oyster, 11-16 parts of soft cooked rice noodles, 11-16 parts of Chinese mugwort leafmulberry leaf, 5-10 parts of plant ash, prescription comprises the following components in parts by weight: 3-10 parts of realgar, 3-10 parts of wrinkled giant hyssop, 3-10 parts of the Chinese bulbul, 5-13 parts of purslane, 3-10 parts of Cortex Phellodendri, 5-13 parts of thalictrum root, myrobalan 5-13 Part, 8-15 parts of stop dysentery grass, 5-13 parts of radix scutellariae, 5-13 parts of the bark of ash, 5-13 parts of wintergreen, 8-15 parts of dandelion.Chinese herbal feed additive provided by the invention can reduce the chick death rate, improve the immunocompetence and antibacterial ability of chick, improve growth performance.
Description
Technical field
The invention belongs to chicken feed addictive fields, specifically, being related to a kind of improvement infection salmonella chick growth
The Chinese herbal feed additive of performance.
Background technique
Salmonella is that livestock and poultry is caused to fall ill, and endangers one of pathogen the most serious of aquaculture, while being also to pass through
Food infects the mankind, causes one of the main pathogen of human foods poisoning.In livestock and poultry death resulting from and caused food
Malicious event has given many development to neutralize developed country and has caused huge economic loss and public health crisis, so that Salmonella
Bacterium becomes one and is given more sustained attention and bone of contention.The U.S. occur every year about 2,000,000 causes eat it is salmonella-polluted
The case for the human infection that animal products causes, the expense for treatment are up to 1,400,000,000 dollars.
Birds are the natural reservoir (of bird flu viruses) of salmonella, mainly cause chick morbidity and death after infection, laying hen is in laying rate more
Decline and peritonitis symptom.Infected chicken constantly outwardly propagates germ by feather, excrement and egg.Studies have shown that Salmonella
In the food poisoning of bacterium infected chicken, 50% can detect that Bacterium enteritidis, and 40%-80% food poisoning is
Caused by fowl Bacterium enteritidis.
Since the perpendicular and horizontal two ways of the disease is propagated, and poultry subclinical infection cannot be found in time, so that control
Make and solve the salmonella-polluted problem that becomes international.It is mostly controlled at present using purification means and the method for feeding antibiotic
It is salmonella-polluted, but these control measure are unsatisfactory, and since a large amount of uses of antibiotic cause antibody-resistant bacterium constantly to go out
It is now serious with antibiotic residue problem.And Chinese herbal medicine is of long standing and well established as a kind of history that drug uses in China, many middle beasts
Medicine has been developed, and clinical application has shown that Chinese herbal medicine has preferable therapeutic effect to some bacteriosis.In
Containing complicated effective component in medicine, different Chinese medicines can play synergistic effect and inhibit or kill pathogen.In recent years, Chinese medicine
Bacteriostasis research work has obtained good development, and Chinese medicine is demonstrated by the unexistent advantage of antibiotic in terms of inhibiting bacterium,
Most obvious one is that Chinese medicine inhibits that bacterium can not be made to generate drug resistance while bacterium, and there are also disease-resistant in vivo for some Chinese medicine
The effect of poison, and the resistance of body can be enhanced in Chinese medicine, adjusts the immune system of body, enhances the cellular immunity and body of body
Liquid immunization, in addition Chinese medicine has the antibacterial action of wide spectrum, so Chinese medicine is the very promising selection of inhibition bacterium.Mesh
Before, Chinese herbal feed additive is more and more applied in poultry or livestock culturing, but targetedly Chinese herbal medicine addition
Agent type and dosage need further to probe into.
Summary of the invention
In order to overcome the problems in background technique, this application provides a kind of improvement to infect salmonella growth performance of chicks
Chinese herbal feed additive, can reduce the chick death rate, improve the immunocompetence and antibacterial ability of chick, improve growth
Energy.
To achieve the above object, the present invention is implemented according to following technical scheme:
A kind of Chinese herbal feed additive improving infection salmonella growth performance of chicks, including square foundation and prescription,
By weight calculating square foundation: prescription=1:1.
The square foundation comprises the following components in parts by weight: 10-15 parts of Radix Angelicae Sinensis, 10-15 parts of fructus lycii, and Chinese yam 10-15
Part, 1-6 parts of Radix Salviae Miltiorrhizae, 10-15 parts of Schisandra chinensis, 2-8 parts of corydalis tuber, 3-9 parts of frutus cnidii, 2-8 parts of oyster, 11-16 parts of soft cooked rice noodles, fry Chinese mugwort
11-16 parts of leaf, 5-10 parts of plant ash.
The prescription comprises the following components in parts by weight: 3-10 parts of realgar, 3-10 parts of wrinkled giant hyssop, and 3-10 parts of the Chinese bulbul, horse
5-13 parts of bitterroot, 3-10 parts of Cortex Phellodendri, 5-13 parts of thalictrum root, 5-13 parts of myrobalan, 8-15 parts of stop dysentery grass, 5-13 parts of radix scutellariae, bark of ash 5-
13 parts, 5-13 parts of wintergreen, 8-15 parts of dandelion.
A kind of preparation method for the Chinese herbal feed additive improving infection salmonella growth performance of chicks, specific steps
Are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus
Water carries out ultrasonic immersion, and amount of water is 6-10 times, soaking time 30-60 minutes of compound prescription weight；
2) it completes to extract repeatedly 3 times after ultrasound is impregnated, each extraction time is 45-60 minutes, will extract every time filtrate and mix
It closes uniform；
3) the resulting filtrate of step 2) is condensed into medicinal extract, Chinese herbal feed is then made using ultralow temperature spray drying and is added
Add agent.
Beneficial effects of the present invention: for the present invention using traditional Chinese medicine principle and poultry threpsology as foundation, providing one kind can
The death rate for reducing the chick of infection salmonella, improves the immunocompetence and antibacterial ability of chick, improves in growth performance
Herbal medicine feed addictive；And using the technique of ultrasound extraction, the recovery rate of aqueous soluble active constituent is improved, reduction was being extracted
The loss of effective component in journey.
Specific embodiment
Embodiment 1
A kind of Chinese herbal feed additive improving infection salmonella growth performance of chicks, including square foundation and prescription,
By weight calculating square foundation: prescription=1:1,5 kilograms of 5 kilograms+prescription of 10 kilograms of compound prescription=square foundations.
Wherein, the square foundation comprises the following components in parts by weight: 15 parts of Radix Angelicae Sinensis, 10 parts of fructus lycii, 13 parts of Chinese yam, red
5 parts, 15 parts of Schisandra chinensis, 2 parts of corydalis tuber, 3 parts of frutus cnidii, 8 parts of oyster, 15 parts of soft cooked rice noodles, 11 parts of Chinese mugwort leafmulberry leaf, 10 parts of plant ash of ginseng；
The prescription comprises the following components in parts by weight: 3 parts of realgar, 3 parts of wrinkled giant hyssop, and 8 parts of the Chinese bulbul, 13 parts of purslane, 10 parts of Cortex Phellodendri,
5 parts of thalictrum root, 5 parts of myrobalan, 15 parts, 13 parts of radix scutellariae, 10 parts of the bark of ash, 10 parts of wintergreen, 8 parts of dandelion of stop dysentery grass.
Preparation method are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus
Water carries out ultrasonic immersion, and amount of water is 6 times, soaking time 30 minutes of compound prescription weight；
2) it completes to extract repeatedly 3 times after ultrasound is impregnated, each extraction time is 45 minutes, and it is equal will to extract filtrate mixing every time
It is even；
3) the resulting filtrate of step 2) is condensed into medicinal extract, Chinese herbal feed is then made using ultralow temperature spray drying and is added
Add agent.
Embodiment 2
A kind of Chinese herbal feed additive improving infection salmonella growth performance of chicks, including square foundation and prescription,
By weight calculating square foundation: prescription=1:1,5 kilograms of 5 kilograms+prescription of 10 kilograms of compound prescription=square foundations.
Wherein, the square foundation comprises the following components in parts by weight: 10 parts of Radix Angelicae Sinensis, 12 parts of fructus lycii, and 10 parts of Chinese yam, Radix Salviae Miltiorrhizae
6 parts, 10 parts of Schisandra chinensis, 8 parts of corydalis tuber, 9 parts of frutus cnidii, 2 parts of oyster, 11 parts of soft cooked rice noodles, 16 parts of Chinese mugwort leafmulberry leaf, 5 parts of plant ash；Institute
The prescription stated comprises the following components in parts by weight: 10 parts of realgar, 10 parts of wrinkled giant hyssop, and 6 parts of the Chinese bulbul, 5 parts of purslane, 10 parts of Cortex Phellodendri,
13 parts of thalictrum root, 5 parts of myrobalan, 8 parts, 5 parts of radix scutellariae, 13 parts of the bark of ash, 13 parts of wintergreen, 10 parts of dandelion of stop dysentery grass.
Preparation method are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus
Water carries out ultrasonic immersion, and amount of water is 8 times, soaking time 50 minutes of compound prescription weight；
2) it completes to extract repeatedly 3 times after ultrasound is impregnated, each extraction time is 60 minutes, and it is equal will to extract filtrate mixing every time
It is even；
3) the resulting filtrate of step 2) is condensed into medicinal extract, Chinese herbal feed is then made using ultralow temperature spray drying and is added
Add agent.
Embodiment 3
A kind of Chinese herbal feed additive improving infection salmonella growth performance of chicks, including square foundation and prescription,
By weight calculating square foundation: prescription=1:1,5 kilograms of 5 kilograms+prescription of 10 kilograms of compound prescription=square foundations.
Wherein, the square foundation comprises the following components in parts by weight: 12 parts of Radix Angelicae Sinensis, 12 parts of fructus lycii, 15 parts of Chinese yam, red
3 parts, 10 parts of Schisandra chinensis, 8 parts of corydalis tuber, 5 parts of frutus cnidii, 5 parts of oyster, 14 parts of soft cooked rice noodles, 16 parts of Chinese mugwort leafmulberry leaf, 6 parts of plant ash of ginseng；
The prescription comprises the following components in parts by weight: 6 parts of realgar, 6 parts of wrinkled giant hyssop, and 6 parts of the Chinese bulbul, 10 parts of purslane, 10 parts of Cortex Phellodendri,
10 parts of thalictrum root, 9 parts of myrobalan, 10 parts, 10 parts of radix scutellariae, 5 parts of the bark of ash, 5 parts of wintergreen, 15 parts of dandelion of stop dysentery grass.
Preparation method are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus
Water carries out ultrasonic immersion, and amount of water is 10 times, soaking time 60 minutes of compound prescription weight；
2) it completes to extract repeatedly 3 times after ultrasound is impregnated, each extraction time is 60 minutes, and it is equal will to extract filtrate mixing every time
It is even；
3) the resulting filtrate of step 2) is condensed into medicinal extract, Chinese herbal feed is then made using ultralow temperature spray drying and is added
Add agent.
Experimental analysis
Chinese herbal feed additive prepared by 2 Chinese herbal medicine formula of embodiment is applied in experimental group.
Experimental chicken chooses 240 1 age in days section treasured broiler chicken, is randomly divided into 4 groups, every group 60, respectively control group is (in being not added
Herbal medicine feed addictive), experimental group 1 (Chinese herbal feed additive additive amount is 1%), 2 (Chinese herbal feed additive of experimental group
3%) and experimental group 3 (Chinese herbal feed additive additive amount be 5%) additive amount is.To experimental group when raising to 2 week old
Test chicken gavages Bacterium enteritidis, and after gavaging for 24 hours, these three time points of 48h, 72h carry out slaughter experiment respectively, compare
Group and three experimental groups butcher 10 every time.All experimental group basal diet formulas are identical with trophic level, and test chicken basis is raised
It is raw material that grain, which selects corn, dregs of beans, expanded soybean etc., and trophic level referring to Chinese fowl raising standard (2004), match by Basic drawing
Fang Jianbiao 1.
1 daily ration of table composition and trophic level
Note: mineral additive provides for every kg diet: iron 80.00mg；Copper 20.00mg；Manganese 80.00mg；Zinc 100.00
mg；Iodine 0.40mg；Selenium 0.30mg.
Gavage and raised according to conventional feeding after Bacterium enteritidis, observe for three days on end the mental status of test chicken, appetite,
The clinical changes such as activity, excrement and morbidity and death condition；After infection for 24 hours, 48h and 72h carry out following detection: butcher and take
Sample measures growth performance index: the daily feeding forage volume of record weighs test chicken weight before butchering respectively, calculates experimental period
Be averaged feed consumption, average daily gain and feed-weight ratio daily；Venous blood collection is carried out to each group chicken, using heparin sodium as anticoagulant
Agent carries out blood routine detection with routine analysis of blood instrument, predominantly detects after infection that the blood such as red blood cell, leucocyte are normal in each group chicken blood
Advise the quantity variation of cell；The measurement of Immunoglobulin IgA, IgG, IgM and Complement C_3, C4.
Experimental result
Inhibiting effect of 2 Chinese herb feed additive of table to salmonella infection
Note: same column shoulder mark capitalization is different, and difference is extremely significant (p < 0.01)；Same column shoulder mark lowercase is different, difference
Significantly (p < 0.05)
As can be seen from Table 2, compared with the control group, three experimental groups significantly increase the survival rate of experimental chicken, reduce
The death rate of experimental chicken.Wherein, the survival rate of experimental group 2 is extremely significant is higher than experimental group 1 and experimental group 3 (p < 0.01), experiment
The death rate of group 2 is extremely significant to be lower than experimental group 1 and experimental group 3 (p < 0.01), and the survival rate highest of experimental group 2, the death rate is minimum.
There is the section's treasured broiler chicken survival rate for improving and being infected by Bacterium enteritidis using Chinese herbal feed additive of the invention, reduce dead
Die the effect of rate.
Influence (g) of 3 Chinese herb feed additive of table to the test chicken live-weight variation of salmonella infection
Note: same column shoulder mark capitalization is different, and difference is extremely significant (p < 0.01)；Same column shoulder mark lowercase is different, difference
Significantly (p < 0.05)
As can be seen from Table 3, the experimental chicken live-weight of three experimental groups after infection for 24 hours, 48h and 72h is extremely significant is higher than
Control group (p < 0.01), control group weight gain after being infected by Bacterium enteritidis is slow, and the live-weight of remaining experimental group increases
Add faster, experimental group 2 live-weight and daily gain after being infected by Bacterium enteritidis is extremely significant higher than experimental group 1 and experimental group 3
(p <0.01).Have the section's treasured broiler chicken for improving and being infected by Bacterium enteritidis living using Chinese herbal feed additive of the invention
The effect of weight and daily gain, wherein 2 effect of experimental group is best.
Influence of 4 Chinese herb feed additive of table to test chicken feed efficiency (feed-weight ratio)
Note: same column shoulder mark capitalization is different, and difference is extremely significant (p < 0.01)；Same column shoulder mark lowercase is different, difference
Significantly (p < 0.05)
As can be seen from Table 4, the test chicken feed efficiency of three experimental groups the 1st day after infection, the 2nd day and the 3rd
It is substantially less than control group (p < 0.05), and the feed efficiency of experimental group 2 the 1st day after infection, the 2nd day and the 3rd day
It is substantially less than other two experimental groups (p < 0.05).Being had using Chinese herbal feed additive of the invention is reduced by enteritis sand
The effect of section's treasured broiler fodder transformation efficiency of door Salmonella infection, wherein 2 effect of experimental group is best.
Influence (%) of 5 Chinese herb feed additive of table to test chicken shoot formation after infection for 24 hours
Note: same column shoulder mark capitalization is different, and difference is extremely significant (p < 0.01)；Same column shoulder mark lowercase is different, difference
Significantly (p < 0.05)
As can be seen from Table 5, the index and spleen index of three experimental group test chickens and liver index be substantially less than control group (p <
0.05), and thymus index and bursal index are all remarkably higher than control group (p < 0.05), wherein the index and spleen index of experimental group 2 and
Liver index is minimum, and thymus index and bursal index highest.Being had using Chinese herbal feed additive of the invention is reduced
The section's treasured broiler chicken index and spleen index and liver index infected by Bacterium enteritidis, improves the work of thymus index and bursal index
With.
Influence of 6 Chinese herb feed additive of table to test chicken routine blood indexes variation after infection for 24 hours
Note: colleague's shoulder mark capitalization is different, and difference is extremely significant (p < 0.01)；Shoulder mark lowercase of going together is different, difference
Significantly (p < 0.05)
As can be seen from Table 6, each group test chicken erythrocyte number after Bacterium enteritidis infection has different degrees of drop
Low, the red blood cell number of three experimental groups is all remarkably higher than control group (p < 0.01), wherein the red blood cell number highest of experimental group 2, together
When each group test chicken the metainfective quantity of leucocyte of Bacterium enteritidis with infection before compared with increased, three experimental groups
Quantity of leucocyte be substantially less than control group (p < 0.05), wherein the quantity of leucocyte of experimental group 2 is minimum, uses the present invention
Chinese herb feed additive can reduce invasion of the pathogen to body, reduce the amplitude of White blood cell.
Influence of 7 Chinese herb feed additive of table to immunoglobulin and complement variation in chicken blood is tested after infection for 24 hours
Note: colleague's shoulder mark capitalization is different, and difference is extremely significant (p < 0.01)；Shoulder mark lowercase of going together is different, difference
Significantly (p < 0.05)
As can be seen from Table 7, the Immunoglobulin IgA, IgG, IgM and Complement C_3, C4 of three experimental groups are different degrees of
Be lower than control group, wherein the immunoglobulin level of experimental group 2 is minimum, and experimental group 3 is taken second place, and uses Chinese herbal medicine of the invention
The section's treasured broiler chicken immunoglobulin and level of complement that there is feed addictive reduction to be infected by Bacterium enteritidis improve body and exempt from
The effect of epidemic disease power.
The present invention provides a kind of can reduce and infects salmonella using traditional Chinese medicine principle and poultry threpsology as foundation
The death rate of chick improves the immunocompetence of chick and the Chinese herbal feed additive of antibacterial ability；Simultaneously using ultrasound extraction
Technique, reduce the loss of effective component during the extraction process, improve the recovery rate of aqueous soluble active constituent.
Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (4)
1. a kind of Chinese herbal feed additive for improving infection salmonella growth performance of chicks, it is characterised in that: described changes
The Chinese herbal feed additive of sentimental dye salmonella growth performance of chicks includes square foundation and prescription, by weight calculating basis
Side: prescription=1:1.
2. a kind of Chinese herbal feed additive for improving infection salmonella growth performance of chicks according to claim 1,
It is characterized by: the square foundation comprises the following components in parts by weight: 10-15 parts of Radix Angelicae Sinensis, 10-15 parts of fructus lycii, Chinese yam 10-15
Part, 1-6 parts of Radix Salviae Miltiorrhizae, 10-15 parts of Schisandra chinensis, 2-8 parts of corydalis tuber, 3-9 parts of frutus cnidii, 2-8 parts of oyster, 11-16 parts of soft cooked rice noodles, fry Chinese mugwort
11-16 parts of leaf, 5-10 parts of plant ash.
3. a kind of Chinese herbal feed additive for improving infection salmonella growth performance of chicks according to claim 1,
It is characterized by: the prescription comprises the following components in parts by weight: 3-10 parts of realgar, 3-10 parts of wrinkled giant hyssop, 3-10 parts of the Chinese bulbul,
5-13 parts of purslane, 3-10 parts of Cortex Phellodendri, 5-13 parts of thalictrum root, 5-13 parts of myrobalan, 8-15 parts of stop dysentery grass, 5-13 parts of radix scutellariae, the bark of ash
5-13 parts, 5-13 parts of wintergreen, 8-15 parts of dandelion.
4. a kind of Chinese herbal medicine of improvement infection salmonella growth performance of chicks according to claim 1 to 3 is raised
The preparation method of feed additives, it is characterised in that: specific steps are as follows:
1) square foundation and each raw material of prescription are weighed respectively, is mixed to get compound prescription by weight 1:1, by compound prescription plus water into
Row ultrasound is impregnated, and amount of water is 6-10 times, soaking time 30-60 minutes of compound prescription weight；
2) it completes to extract repeatedly 3 times after ultrasound is impregnated, each extraction time is 45-60 minutes, and it is equal will to extract filtrate mixing every time
It is even；
3) the resulting filtrate of step 2 is condensed into medicinal extract, Chinese herbal feed addition is then made using ultralow temperature spray drying
Agent.
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| CN201910052782.6A CN109691595A (en) | 2019-01-21 | 2019-01-21 | A kind of Chinese herbal feed additive improving infection salmonella growth performance of chicks |
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Family
ID=66234215
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| CN105192367A (en) * | 2015-09-25 | 2015-12-30 | 南宁源广农业科技有限公司 | Medicated food chicken feed |
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| CN108497169A (en) * | 2018-04-02 | 2018-09-07 | 尚洋 | A kind of livestock and poultry Chinese herb feed additive and preparation method thereof |
| CN108651759A (en) * | 2018-07-26 | 2018-10-16 | 石阡县毅峰农牧开发有限公司 | A kind of chicken feed additive and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1133131A (en) * | 1995-04-10 | 1996-10-16 | 李银龙 | Chinese medicinal herbs fodder additive and preparation process thereof |
| CN102988548A (en) * | 2011-09-19 | 2013-03-27 | 天津瑞贝特科技发展有限公司 | Chinese medicinal composition with function of astringing intestine to stop diarrhea, preparation method thereof |
| CN102640864A (en) * | 2012-02-23 | 2012-08-22 | 上海交通大学 | Chinese medicinal herb feed additive for improving immunity of chickens and preventing diarrhea and using method for additive |
| JP2014183751A (en) * | 2013-03-22 | 2014-10-02 | Akita Prefectural Univ | Bacteria that inhibit avian salmonella and viable bacterial agent utilizing them |
| CN105192367A (en) * | 2015-09-25 | 2015-12-30 | 南宁源广农业科技有限公司 | Medicated food chicken feed |
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| CN108497169A (en) * | 2018-04-02 | 2018-09-07 | 尚洋 | A kind of livestock and poultry Chinese herb feed additive and preparation method thereof |
| CN108651759A (en) * | 2018-07-26 | 2018-10-16 | 石阡县毅峰农牧开发有限公司 | A kind of chicken feed additive and preparation method thereof |
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