CN109678258A - 一种提高菲降解菌降解效率的微量营养素 - Google Patents
一种提高菲降解菌降解效率的微量营养素 Download PDFInfo
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- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
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Abstract
本发明公开了一种提高菲降解菌降解效率的微量营养素,属于食品食料领域,其由以下质量百分比的原料配成:氨基酸5~7%、维生素15~17%、无机盐14~16%,其余为水。此营养素中特定配比的无机盐能够促进细胞表面分泌更多的胞外聚合物,一方面形成抵御有毒物质的保护层,能维持菲降解菌的生命力;另一方面能够对疏水性的菲具有很强的吸附力,增加菲降解菌对菲的吸附性,并形成特殊的包膜,将不溶性的菲转化到菲降解菌细胞内,进而将菲分解掉;特定的氨基酸则为上述过程提供能量,特定的维生素为菲降解菌提供生长必需的生长因子,从而通过共同作用来有效地提高菲降解菌的降解活性,起到提高菲降解菌对含菲废水处理效率的目的。
Description
技术领域
本发明属于食品食料技术领域,具体涉及一种提高菲降解菌降解效率的微量营养素。
背景技术
目前,随着我国工业技术发展进程的加快,工业废水的排放量日益增加,并且越来越多的工业废水中含有难降解的多环芳烃化合物。多环芳烃(Polycyclic aromatichydrocarbon,简称PAHs)在环境中随处可见,大部分的PAHs具有致癌、致畸、致突变效应,严重危害着人类的身体健康。
菲是一种常见的多环芳烃化合物,自然界中的菲来源于化石燃料、生物合成、沉积岩风化等,而人为活动是菲的主要来源,尤其是随着工业的发展,药物、燃料、橡胶、塑料、农药生产过程中的中间产物,以及煤炭、石油、烟草等的不完全燃烧都会产生菲。
针对水体中菲的化学特性,国内外处理含菲废水的方法主要有微生物处理法、物理吸附法、臭氧氧化法等技术。其中,微生物处理法因具有环境友好、成本低等优点而被广泛使用。微生物处理法是指利用菲降解菌将环境中的菲作为碳源和能源进行利用,从而将菲降解为二氧化碳和水等无毒物质。但由于菲的化学特性,其对菲降解菌的生长具有一定的毒性,使菲降解菌降解菲的效率不高,从而降低了菲降解菌对含菲废水的处理效率。
发明内容
本发明所要解决的技术问题是提供一种提高菲降解菌降解效率的微量营养素,能够提高菲降解菌的降解活性。
为解决上述技术问题,本发明的技术方案是:设计其特征在于:由以下质量百分比的原料配成:
氨基酸 5~6%
维生素 15~16%
无机盐 14~15%
其余为水;
所述氨基酸由以下组分按质量百分比构成:
甘氨酸 35%
赖氨酸 20%
丝氨酸 25%
脯氨酸 20%
所述维生素由以下组分按质量百分比构成:
维生素A 40%
维生素C 20%
维生素E 15%
生物素 25%
所述无机盐由以下组分按质量百分比构成:
Na2HPO4 45%
KH2PO4 20%
ZnSO4 5%
MnSO4 8%
FeSO4 12%
CuSO4 10%。
优选的,由以下质量百分比的原料配成:
氨基酸 6%
维生素 16%
无机盐 15%
其余为水。
与现有技术相比,本发明的有益效果是:
1、本发明中特定配比的无机盐能够作为细胞膜上通道打开的催化剂,有效地将在细胞内合成的胞外聚合物运输到细胞外,促进细胞表面分泌更多的胞外聚合物,一方面表面亲和性强的胞外聚合物附着在菲降解菌表面,形成抵御有毒物质的保护层,能维持菲降解菌的生命力;另一方面胞外聚合物具有一些疏水结构功能区,这些功能区对疏水性的菲具有很强的吸附力,从而增加菲降解菌对菲的吸附性,并形成特殊的包膜,将不溶性的菲转化到菲降解菌细胞内,进而将菲分解掉;而在细胞内合成上述胞外聚合物的过程是一个需要消耗ATP的过程,特定配比的氨基酸恰好提供该能量,而特定配比的维生素则为菲降解菌提供生长必需的生长因子,从而通过共同作用来有效地提高菲降解菌的降解活性,起到提高菲降解菌对含菲废水处理效率的目的。
2、该营养素能为菲降解菌提供必需的营养物质,从而以最小的成本投入换来比较理想的效果。
附图说明
图1为实验组与对照组的菲降解菌生物量对比图;
图2为实验组与对照组的菲残余量对比图。
具体实施方式
下面结合具体实施方式对本发明作进一步详细描述。
本发明所提供的一种提高菲降解菌处理含菲废水效率的微量营养素,在菲降解菌降解菲的过程中,为菲降解菌提供细胞生长所需的微量营养素组合,最终提高菲降解菌的降解活性,提升含菲废水的处理效果。
实施例一
本实施例中的微量营养素由氨基酸、维生素、无机盐和水四种原料构成,其中氨基酸占营养素总质量的5%,维生素占营养素总质量的15%,无机盐占营养素总质量的14%,其余为水。
所用氨基酸由甘氨酸、赖氨酸、丝氨酸和脯氨酸组成,其中甘氨酸占氨基酸总质量的35%、赖氨酸占氨基酸总质量的20%,丝氨酸占氨基酸总质量的25%,脯氨酸占氨基酸总质量的20%。
所用维生素由维生素A、维生素C、维生素E和生物素组成。其中维生素A 占维生素总质量的40%,维生素C占维生素总质量的20%,维生素E占维生素总质量的 15%,生物素占维生素总质量的25%。
所用无机盐由Na2HPO4、KH2PO4、ZnSO4、MnSO4、FeSO4和CuSO4组成。其中Na2HPO4占无机盐总质量的45%,KH2PO4占无机盐总质量的20%,ZnSO4占无机盐总质量的5%,MnSO4占无机盐总质量的8%,FeSO4占无机盐总质量的12%,CuSO4占无机盐总质量的10%。
实施例二
本实施例与实施例一的不同之处在于:本实施例中氨基酸占营养素总质量的6%,维生素占营养素总质量的16%,无机盐占营养素总质量的15%,其余为水。其余均同实施例一。
实施例三
本实施例与实施例一的不同之处在于:本实施例中氨基酸占营养素总质量的7%,维生素占营养素总质量的17%,无机盐占营养素总质量的16%,其余为水。其余均同实施例一。
本发明提供的微量营养素为菲降解菌提供了在降解菲过程中所需的能量和微量元素,促使菲降解菌合成并分泌更多的胞外聚合物,增加对菲的吸附和摄取能力,增强菲降解菌对菲的降解活性,同时维持菲降解菌菌群的稳定性。此外,微量营养素添加剂中的微量金属元素,可以作为菲降解菌细胞内菲代谢通路中关键酶的催化剂,进而提升菲降解菌对菲的降解效率。
发明人做了多次测试实验,测试过程如下:
将反应器分为实验组和对照组。实验组和对照组测试条件相同,包括具有相同的菲降解菌接种量、菲含量相同的废水、反应体积、反应温度、曝气量等。实验组添加所述微量营养素,对照组则不添加。所述微量营养素的添加方式为每小时向反应器内加入15ppm的微量营养素,连续加3次,24小时后分别检测实验组和对照组反应器内菲降解菌的生物量和菲残余量,实验结果一致,只是数值的细微差别,从中随意选择一组作为代表,具体见图1和图2。
从图1和图2中可以看出,添加本实施例的微量营养素后,实验组废水中的菲降解菌的生物量比对照组高出三倍以上,实验组废水中的菲残余量比对照组低出二倍以上,效果非常明显。在研发的过程中,也采用过其它配方组成的营养素,均没有达到过如此好的效果。
以上所述,仅是本发明的较佳实施例而已,并非是对本发明作其它形式的限制,任何熟悉本专业的技术人员可能利用上述揭示的技术内容加以变更或改型为等同变化的等效实施例。但是凡是未脱离本发明技术方案内容,依据本发明的技术实质对以上实施例所作的任何简单修改、等同变化与改型,仍属于本发明技术方案的保护范围。
Claims (2)
1.一种高性能聚酯醇解溶液,其特征在于:由以下质量百分比的原料配成:
氨基酸 5~7%
维生素 15~17%
无机盐 14~16%
其余为水;
所述氨基酸由以下组分按质量百分比构成:
甘氨酸 35%
赖氨酸 20%
丝氨酸 25%
脯氨酸 20%
所述维生素由以下组分按质量百分比构成:
维生素A 40%
维生素C 20%
维生素E 15%
生物素 25%
所述无机盐由以下组分按质量百分比构成:
Na2HPO4 45%
KH2PO4 20%
ZnSO4 5%
MnSO4 8%
FeSO4 12%
CuSO4 10%。
2.根据权利要求1所述的高性能聚酯醇解溶液,其特征在于:由以下质量百分比的原料配成:
氨基酸 6%
维生素 16%
无机盐 15%
其余为水。
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