CN109678255A - A kind of culture experiment device of the simulation redox fluctuation adjusted based on nitrogen oxygen - Google Patents
A kind of culture experiment device of the simulation redox fluctuation adjusted based on nitrogen oxygen Download PDFInfo
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- CN109678255A CN109678255A CN201811653319.9A CN201811653319A CN109678255A CN 109678255 A CN109678255 A CN 109678255A CN 201811653319 A CN201811653319 A CN 201811653319A CN 109678255 A CN109678255 A CN 109678255A
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- 238000002474 experimental method Methods 0.000 title claims abstract description 50
- DOTMOQHOJINYBL-UHFFFAOYSA-N molecular nitrogen;molecular oxygen Chemical compound N#N.O=O DOTMOQHOJINYBL-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 238000004088 simulation Methods 0.000 title claims abstract description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 100
- 239000007789 gas Substances 0.000 claims abstract description 58
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 53
- 239000001301 oxygen Substances 0.000 claims abstract description 53
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 53
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 50
- 238000000746 purification Methods 0.000 claims abstract description 29
- 238000012545 processing Methods 0.000 claims abstract description 28
- 238000006243 chemical reaction Methods 0.000 claims description 66
- 238000012544 monitoring process Methods 0.000 claims description 14
- 238000005070 sampling Methods 0.000 claims description 13
- 239000012295 chemical reaction liquid Substances 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- 238000003760 magnetic stirring Methods 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- 238000003780 insertion Methods 0.000 claims description 6
- 230000037431 insertion Effects 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 230000001737 promoting effect Effects 0.000 claims description 3
- 230000006837 decompression Effects 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 18
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 6
- 229910052753 mercury Inorganic materials 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 230000033116 oxidation-reduction process Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 3
- 229910001882 dioxygen Inorganic materials 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- JJWSNOOGIUMOEE-UHFFFAOYSA-N Monomethylmercury Chemical compound [Hg]C JJWSNOOGIUMOEE-UHFFFAOYSA-N 0.000 description 2
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000009970 fire resistant effect Effects 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/18—Gas cleaning, e.g. scrubbers; Separation of different gases
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/04—Oxidation reduction potential [ORP]
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/22—O2
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/38—Gas flow rate
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Abstract
The present invention provides a kind of culture experiment devices of simulation redox fluctuation adjusted based on nitrogen oxygen, including sequentially connected nitrogen oxygen source device, gas source purification processing unit, gas distributing device, culture experiment device and exhaust gas processing device；The nitrogen oxygen source device includes nitrogen cylinder and oxygen cylinder, for providing nitrogen, carrier of oxygen；The gas source purification processing unit purifies the nitrogen, the carrier of oxygen that enter culture experiment device；The gas distributing device is used to adjust the environment of nitrogen, carrier of oxygen flow to simulate redox fluctuation；The culture experiment device is the reactive moieties of culture experiment；The exhaust gas processing device handles issuable various tail gas.The device of the invention can be used for the culture experiment research under a variety of redox surging conditions, provide effective device further to disclose associated biomolecule geochemical process under redox surging condition.
Description
Technical field
The present invention relates to groundwater simulation technical field more particularly to a kind of simulation redox waves adjusted based on nitrogen oxygen
Dynamic culture experiment device.
Background technique
In nature-especially in redox interacting strip-redox fluctuation it is ubiquitous, dry season wet season
SEA LEVEL VARIATION, Reservoir Regulation water storage, tide and day and night temperature etc. can all cause redox to be fluctuated；And redox influence of fluctuations
Biogeochemical process, or even decide the distribution of such as nutrient in redox interacting strip return, pollutant
The processes such as Transport And Transformation, the community distribution of microorganism and microbial activities situation.In order to preferably study these biogeochemicals
Process has gradually carried out the culture experiment under redox surging condition.
Preferably to simulate the culture experiment environment under redox fluctuation in laboratory conditions, need to design a kind of mould
The experiment culture apparatus of quasi- redox fluctuation, can be used in the culture experiment research under a variety of redox surging conditions, is
It further discloses associated biomolecule geochemical process under redox surging condition and effective device is provided.
Summary of the invention
It is adjusted in view of this, that the present invention provides a kind of structures is simple, simulation effect is good, feasibility is high based on nitrogen oxygen
Simulate the experiment culture apparatus of redox fluctuation.
The present invention provides a kind of culture experiment devices of simulation redox fluctuation adjusted based on nitrogen oxygen, including successively
Nitrogen oxygen source device, gas source purification processing unit, gas distributing device and the culture experiment device of connection；The nitrogen source of oxygen dress
It sets including nitrogen cylinder and oxygen cylinder, nitrogen cylinder is used to provide nitrogen, and oxygen cylinder is used to provide oxygen；The gas source purification processing dress
It sets including nitrogen purification bottle group and oxygen purification bottle group, the nitrogen purification bottle group is connected to nitrogen cylinder, is used for nitrogen purge gas, institute
It states oxygen purification bottle group to be connected to oxygen cylinder, is used for purified oxygen；The gas distributing device include several first gas-separating valves and
Several second gas-separating valves, the first gas-separating valve are connected to purified nitrogen, and the second gas-separating valve is connected to purified oxygen；It is described
Culture experiment device includes reaction flask, and the first gas-separating valve is used to be adjusted into the nitrogen amount of reaction flask, and the second gas-separating valve is used to adjust
The whole amount of oxygen into reaction flask, the nitrogen and oxygen of different flow enter reaction flask simulation redox fluctuation environment.
Further, the bottleneck of the nitrogen cylinder and oxygen cylinder reduces gas pressure intensity, the pressure reducing valve by decompression valve regulation
Outlet be connected to gas source purification processing unit.
Further, several triple valves are set between first gas-separating valve, the second gas-separating valve and reaction flask, described first
Gas-separating valve and the second gas-separating valve correspond to a triple valve in pairs, and the two entrances of each triple valve are respectively with corresponding first
Gas-separating valve, the connection of the second gas-separating valve, the outlet of each triple valve is connected to reaction flask.
Further, flowmeter, one end of the flowmeter and threeway are additionally provided between each triple valve and reaction flask
The other end of the outlet of valve, the flowmeter is connected to reaction flask, and the flowmeter is used to measure the gas into reaction flask
Body flow.
Nitrogen, carrier of oxygen in gas source purification processing unit after purified treatment are respectively connected to the first gas-separating valve and second point of gas
Valve, the nitrogen carrier of oxygen separated from the first gas-separating valve and the second gas-separating valve in pairs it is corresponding access one triple valve inlet end,
Then from triple valve outlet side, incoming stream meter, flowmeter are used to measure the gas flow into reaction flask again.Wherein, Suo Youlian
Adapter tube is PU pipe, and connecting interface is fast connection interface, and the inlet, outlet of first, second gas-separating valve is by ball valve switch
Control.First gas-separating valve and the second gas-separating valve can individually adjust the flow velocity of nitrogen, carrier of oxygen, for providing nitrogen, the oxygen of various concentration
Mixed gas carries out multiple experimental groups to simulate different Redox Conditions.
Further, magnetic stirring apparatus is placed in the bottom of the reaction flask, and the magnetic stirring apparatus is for promoting reaction flask
Middle reaction solution is stirred；The reaction flask includes reaction body and reaction bottle cap, and the inside of the reaction body is held instead
Liquid is answered, the reaction flask, which covers, opens up the first aperture, second orifice and third aperture, and air inlet pipe is inserted into the first aperture, described
The upper end of air inlet pipe is connected to the other end of flowmeter, and the reaction liquid level in reaction flask or less is immersed in the lower end of the air inlet pipe；
Escape pipe is inserted into second orifice, the lower end of the escape pipe is more than the reaction liquid level in reaction flask；Insertion in third aperture
Sample feeding pipe is sampled, reaction liquid level or less is immersed in the lower end of the sampling sample feeding pipe.
Further, the upper end of the sampling sample feeding pipe passes to a bottle external application emulsion tube and is connected with one end of two-port valve, described
The other end of two-port valve is connect with syringe, is opened two-port valve and is extracted syringe, that is, sampling, injection syringe can be to reaction
Sample introduction in bottle closes two-port valve after sampling sample introduction, guarantee that mixed gas and the external world obstruct in reaction flask.
Further, the reaction flask, which covers, opens up the 4th aperture, insertion monitoring pipe in the 4th aperture, in the monitoring pipe
Test electrode is placed, the test electrode is put into the parameter for reacting liquid level hereinafter, the test electrode is used to monitor culture experiment,
Such as monitor oxidation-reduction potential, pH and dissolved oxygen amount parameter on-line.
Above-mentioned all pipes into bottle are the polyfluortetraethylene pipe of fire resistant anticorrosive.
Further, the upper end of the escape pipe connects exhaust gas processing device, and the exhaust gas processing device is for handling training
Support the tail gas that experiment generates.The setting of the exhaust gas processing device and the reaction system of culture experiment are related, if culture experiment produces
The gases such as raw chlorine, hydrogen, methane, ammonia, hydrogen sulfide, sulfur dioxide and mercury vapour, should configure corresponding device for absorbing tail gas.
Compared with prior art, technical solution bring provided by the invention has the beneficial effect that:
(1) the device of the invention structure is simple, and feasibility is high, and simulation effect is good, to the training under redox surging condition
Feeding experimental study has very big help；
(2) the device of the invention alternately feeds the motive power fluctuated as redox using nitrogen, carrier of oxygen, and device is simple
It is easy, it is high to the simulation degree of actual conditions；
(3) the device of the invention utilizes air inlet pipe, escape pipe, monitoring pipe and sample introduction in the reaction flask of culture experiment device
Probe tube conveniently and efficiently complete redox fluctuation, system pressure equilibrium, basic parameter on-line monitoring and it is noiseless into
Operation, culture experiment device strong operability, the experiment controls such as sample sampling are accurate；
(4) gas-separating valve individually can simulate redox fluctuation environment by regulating gas flow velocity in turn in apparatus of the present invention, can be same
The multiple experimental groups of Shi Kaizhan.
Detailed description of the invention
Attached drawing is only used for showing the purpose of specific embodiment, and is not to be construed as limiting the invention, in entire attached drawing
In, identical reference symbol indicates identical component.
Fig. 1 is the apparatus structure schematic diagram of the embodiment of the present invention.
Appended drawing reference:
1- nitrogen oxygen source device；1.1- pressure reducing valve；2- gas source purification processing unit；3- gas distributing device；3.1- first
Gas-separating valve；The second gas-separating valve of 3.2-；3.3- flowmeter；3.4- triple valve；4- culture experiment device；4.1- magnetic stirring apparatus；
4.2- reacts body；4.3- reacts bottle cap；4.4- air inlet pipe；4.5- escape pipe；4.6- monitoring pipe；4.7- samples sample feeding pipe；5-
Exhaust gas processing device.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached drawing to embodiment party of the present invention
Formula is further described.
As shown in Figure 1, the embodiment provides a kind of trainings of simulation redox fluctuation adjusted based on nitrogen oxygen
Experimental provision is supported, the rule of mercury Transport And Transformation under redox surging condition has been probed into using the device.
The device includes sequentially connected nitrogen oxygen source device 1, gas source purification processing unit 2, gas distributing device 3, training
Support experimental provision 4 and exhaust gas processing device 5.
The nitrogen oxygen source device 1 includes nitrogen cylinder and oxygen cylinder, is respectively configured to provide nitrogen and oxygen；Nitrogen cylinder and oxygen
The bottleneck of gas cylinder is adjusted by pressure reducing valve 1.1 reduces gas pressure intensity, at the outlet connection PU pipe access gas source purification of pressure reducing valve 1.1
Manage device 2.
The gas source purification processing unit 2 be used for enter below the nitrogen of culture experiment device 3, carrier of oxygen purified and
Exclude experiment interference.Specifically, the gas source purification processing unit 2 includes nitrogen purification bottle group and oxygen purification bottle group, it is described
Nitrogen purification bottle group includes several interconnected nitrogen purification bottles, and the oxygen purification bottle group includes that several are interconnected
Oxygen purification bottle.
The gas distributing device 3 provides redox fluctuation environment for adjusting nitrogen, carrier of oxygen flow.The gas
Body distributor 3 is by several first gas-separating valves 3.1, several second gas-separating valves 3.2, several flowmeters 3.3 and several triple valves
3.4 composition；Nitrogen, carrier of oxygen in gas source purification processing unit 2 after purified treatment are respectively connected to the first, second gas-separating valve, point
The inlet end of nitrogen out, carrier of oxygen corresponding access triple valve 3.4 in pairs, then the outlet side incoming stream of triple valve 3.4
The other end of one end of meter 3.3, flowmeter 3.3 is connect with culture experiment device 4；Wherein, connecting tube is PU pipe, and connection connects
Mouth is fast connection interface.
First, second gas-separating valve can provide the nitrogen of multiple groups various concentration, oxygen gas mixture, and each nitrogen, oxygen gas mixture close
It is correspondingly connected with triple valve, flowmeter, culture experiment device and exhaust gas processing device below, may make up different experimental groups.
The stream of different moments nitrogen, carrier of oxygen is controlled by adjusting the first, second gas-separating valve in embodiments of the present invention
Amount supply, simulates reaction flask internal oxidition reducing environment.Specifically: the second gas-separating valve 3.2 control oxygen open, the first gas-separating valve
3.1 control nitrogen close, and maintain 7 days, then the first gas-separating valve 3.1 control nitrogen is opened, the second gas-separating valve 3.2 control oxygen closes, dimension
It holds 7 days, this process is set as a cycle, repeats this period, causes the fluctuation of Redox Condition in cultivating system.
The culture experiment device 4 be experiment main body reactive moieties, including magnetic stirring apparatus 4.1, reaction body 4.2,
React bottle cap 4.3, air inlet pipe 4.4, escape pipe 4.5, monitoring pipe 4.6 and sampling sample feeding pipe 4.7；Wherein, magnetic stirring apparatus 4.1 is set
Under reaction flask, for promoting the mixing of reaction solution in reaction flask；It reacts body 4.2 and reacts bottle cap 4.3 and constitute reaction flask
Principal space, reaction body 4.2 inside hold the mercury pollution bed mud that contaminated site is fetched be mixed to form with surface water it is suspended
Liquid.The first aperture, second orifice, third aperture and the 4th aperture are opened up on reaction bottle cap 4.3, are inserted into air inlet pipe in the first aperture
4.4, the upper end of the air inlet pipe 4.4 is connected to the other end of flowmeter 3.3, and reaction flask is immersed in the lower end of the air inlet pipe 4.4
Below interior reaction liquid level；Escape pipe 4.5, reaction of the lower end of the escape pipe 4.5 in reaction flask are inserted into second orifice
More than liquid level, the upper end of escape pipe 4.5 is connect with exhaust gas processing device 5, and air inlet pipe 4.4 and 4.5 collective effect of escape pipe are put down
The air pressure of nitrogen, oxygen gas mixture in weighing apparatus reaction flask；Insertion sampling sample feeding pipe 4.7 in third aperture, the sampling sample feeding pipe 4.7
Reaction liquid level is immersed hereinafter, upper end passes to outside bottle in lower end, is connected with emulsion tube with one end of two-port valve, the two-port valve other end with infuse
Emitter connection opens two-port valve and extracts syringe, that is, sampling, injection syringe can reaction solution sample introduction into reaction flask, take
Two-port valve is closed after sample sample introduction, guarantees mixed gas and extraneous barrier in reaction flask；Insertion monitoring pipe in 4th aperture
4.6, the monitoring pipe 4.6 is interior to place test electrode, and the test electrode is put into reaction liquid level hereinafter, for monitoring culture experiment
Parameter, such as parameters such as on-line monitoring oxidation-reduction potential, pH and dissolved oxygen amount.All pipes into bottle are that high temperature resistant is anti-
The polyfluortetraethylene pipe of corrosion.
Different exhaust gas processing devices is arranged based on different tail gas products for the exhaust gas processing device 5.The present invention is implemented
Exhaust gas processing device 5 is methyl mercury, total mercury absorption plant in example.
The embodiment of the present invention monitored experiment culture in reaction flask dissolved oxygen amount, oxidation-reduction potential and pH with nitrogen, oxygen
Body regulating cycle and the case where fluctuating change, specifically: oxygen is opened, nitrogen closes, and in the case where maintaining 7 days, monitors reaction solution
Dissolved oxygen amount increase, oxidation-reduction potential increase, pH reduce；Nitrogen is opened, oxygen closes, and in the case where maintaining 7 days, monitors to react
The dissolved oxygen amount of liquid reduces, and oxidation-reduction potential reduces, and pH is increased；Show that the embodiment of the present invention is simple, efficiently simulates oxidation
Surging condition is restored, while total mercury, methyl mercury content in reaction flask reaction solution in the periodic process are monitored, obtains oxygen
The methylation for changing mercury under the conditions of reduction is fluctuated is enhanced.
The present invention provides a kind of culture experiment device of simulation redox fluctuation adjusted based on nitrogen oxygen, the device knots
Structure is simple, and feasibility is high, and simulation effect is good, has very big help to the culture experiment research under redox surging condition；
Wherein the reaction flask of culture experiment device is conveniently and efficiently completed using air inlet pipe, escape pipe, monitoring pipe and sample introduction probe tube
The operations such as redox fluctuation, system pressure equilibrium, the on-line monitoring of basic parameter and the sampling of noiseless sample introduction, culture experiment dress
Strong operability is set, experiment control is accurate.
Herein, the nouns of locality such as related front, rear, top, and bottom are to be located in figure with components in attached drawing and zero
Part mutual position defines, only for the purpose of expressing the technical solution clearly and conveniently.It should be appreciated that the noun of locality
Use should not limit the claimed range of the application.
In the absence of conflict, the feature in embodiment and embodiment herein-above set forth can be combined with each other.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of culture experiment device of the simulation redox fluctuation adjusted based on nitrogen oxygen, which is characterized in that including successively connecting
Nitrogen oxygen source device, gas source purification processing unit, gas distributing device and the culture experiment device connect；The nitrogen oxygen source device
Including nitrogen cylinder and oxygen cylinder, the nitrogen cylinder is used to provide nitrogen, and the oxygen cylinder is used to provide oxygen；The gas source purification
Processing unit includes nitrogen purification bottle group and oxygen purification bottle group, and the nitrogen purification bottle group is connected to nitrogen cylinder, for purifying
Nitrogen, the oxygen purification bottle group are connected to oxygen cylinder, are used for purified oxygen；The gas distributing device includes several first points
Air valve and several second gas-separating valves, the culture experiment device includes reaction flask, and first gas-separating valve is used to be adjusted into anti-
Answer the nitrogen amount of bottle, second gas-separating valve is used to be adjusted into the amount of oxygen of reaction flask, the nitrogen and oxygen of different flow into
Enter reaction flask simulation redox fluctuation environment.
2. a kind of culture experiment device of simulation redox fluctuation adjusted based on nitrogen oxygen according to claim 1,
Be characterized in that, the bottleneck of the nitrogen cylinder and oxygen cylinder reduces gas pressure intensity by decompression valve regulation, the outlet of the pressure reducing valve with
The connection of gas source purification processing unit.
3. a kind of culture experiment device of simulation redox fluctuation adjusted based on nitrogen oxygen according to claim 1,
Be characterized in that, several triple valves be set between first gas-separating valve, the second gas-separating valve and reaction flask, first gas-separating valve and
Second gas-separating valve in pairs correspond to a triple valve, the two entrances of each triple valve respectively with corresponding first gas-separating valve,
The connection of second gas-separating valve, the outlet of each triple valve is connected to reaction flask.
4. a kind of culture experiment device of simulation redox fluctuation adjusted based on nitrogen oxygen according to claim 3,
It is characterized in that, flow meters, one end of the flowmeter and the outlet of triple valve is also set up between each triple valve and reaction flask
Connection, the other end of the flowmeter are connected to reaction flask, and the flowmeter is used to measure the gas flow into reaction flask.
5. a kind of culture experiment device of simulation redox fluctuation adjusted based on nitrogen oxygen according to claim 4,
It is characterized in that, magnetic stirring apparatus is placed in the bottom of the reaction flask, and the magnetic stirring apparatus is for promoting reaction solution in reaction flask
Be stirred；
The reaction flask includes reaction body and reaction bottle cap, and the inside of the reaction body holds reaction solution, the reaction flask
It covers and opens up the first aperture, second orifice and third aperture, air inlet pipe, the upper end of the air inlet pipe and stream are inserted into the first aperture
The other end of meter is connected to, and the reaction liquid level in reaction flask or less is immersed in the lower end of the air inlet pipe；It is inserted into out in second orifice
Tracheae, the lower end of the escape pipe is more than the reaction liquid level in reaction flask；Insertion sampling sample feeding pipe, described to take in third aperture
Reaction liquid level or less is immersed in the lower end of sample sample feeding pipe.
6. a kind of culture experiment device of simulation redox fluctuation adjusted based on nitrogen oxygen according to claim 5,
It is characterized in that, one end of the upper end connection two-port valve of the sampling sample feeding pipe, the other end of the two-port valve connects syringe, beats
Open two-port valve extract syringe i.e. sampling, injection syringe can into reaction flask sample introduction.
7. a kind of culture experiment device of simulation redox fluctuation adjusted based on nitrogen oxygen according to claim 5,
It is characterized in that, the reaction flask, which covers, opens up the 4th aperture, insertion monitoring pipe in the 4th aperture, places test in the monitoring pipe
Electrode, test electrode are put into reaction liquid level hereinafter, being used to monitor the parameter of culture experiment.
8. a kind of culture experiment device of simulation redox fluctuation adjusted based on nitrogen oxygen according to claim 5,
It is characterized in that, the upper end of the escape pipe connects exhaust gas processing device, and the exhaust gas processing device is for handling culture experiment production
Raw tail gas.
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Application publication date: 20190426 |