CN109646464A - A kind of pycnoporus samguineus extract and its extracting method and application - Google Patents
A kind of pycnoporus samguineus extract and its extracting method and application Download PDFInfo
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- CN109646464A CN109646464A CN201811604777.3A CN201811604777A CN109646464A CN 109646464 A CN109646464 A CN 109646464A CN 201811604777 A CN201811604777 A CN 201811604777A CN 109646464 A CN109646464 A CN 109646464A
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- pycnoporus samguineus
- samguineus
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- 241000222644 Pycnoporus <fungus> Species 0.000 title claims abstract description 72
- 239000000284 extract Substances 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 58
- 235000019441 ethanol Nutrition 0.000 claims abstract description 25
- 239000000469 ethanolic extract Substances 0.000 claims abstract description 25
- 208000022559 Inflammatory bowel disease Diseases 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000003814 drug Substances 0.000 claims abstract description 13
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 235000013305 food Nutrition 0.000 claims abstract description 7
- 230000036541 health Effects 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 6
- 239000000706 filtrate Substances 0.000 claims description 18
- 239000012141 concentrate Substances 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 13
- 230000001376 precipitating effect Effects 0.000 claims description 12
- 210000004369 blood Anatomy 0.000 claims description 11
- 239000008280 blood Substances 0.000 claims description 11
- 229940079593 drug Drugs 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 239000003643 water by type Substances 0.000 claims description 6
- 238000004090 dissolution Methods 0.000 claims description 4
- 239000000796 flavoring agent Substances 0.000 claims description 4
- 235000019634 flavors Nutrition 0.000 claims description 4
- 241000233866 Fungi Species 0.000 claims description 3
- 238000012869 ethanol precipitation Methods 0.000 claims description 3
- 150000004676 glycans Chemical class 0.000 claims description 3
- 229920001282 polysaccharide Polymers 0.000 claims description 3
- 239000005017 polysaccharide Substances 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 238000007710 freezing Methods 0.000 claims 1
- 238000002386 leaching Methods 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- 210000004953 colonic tissue Anatomy 0.000 abstract description 12
- 206010061218 Inflammation Diseases 0.000 abstract description 7
- 230000002757 inflammatory effect Effects 0.000 abstract description 7
- 230000004054 inflammatory process Effects 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 6
- 238000004108 freeze drying Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 235000013376 functional food Nutrition 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 239000002904 solvent Substances 0.000 abstract description 2
- 229960004756 ethanol Drugs 0.000 description 14
- 244000144993 groups of animals Species 0.000 description 10
- 210000001072 colon Anatomy 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 201000010099 disease Diseases 0.000 description 6
- 208000002551 irritable bowel syndrome Diseases 0.000 description 6
- 239000002158 endotoxin Substances 0.000 description 5
- 229920006008 lipopolysaccharide Polymers 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 210000001744 T-lymphocyte Anatomy 0.000 description 4
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- 239000012466 permeate Substances 0.000 description 4
- 238000010992 reflux Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 210000002443 helper t lymphocyte Anatomy 0.000 description 3
- 210000003289 regulatory T cell Anatomy 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- TVZRAEYQIKYCPH-UHFFFAOYSA-N 3-(trimethylsilyl)propane-1-sulfonic acid Chemical compound C[Si](C)(C)CCCS(O)(=O)=O TVZRAEYQIKYCPH-UHFFFAOYSA-N 0.000 description 2
- 241000221198 Basidiomycota Species 0.000 description 2
- 206010009900 Colitis ulcerative Diseases 0.000 description 2
- 208000011231 Crohn disease Diseases 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 241000222341 Polyporaceae Species 0.000 description 2
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- 210000000447 Th1 cell Anatomy 0.000 description 2
- 210000004241 Th2 cell Anatomy 0.000 description 2
- 201000006704 Ulcerative Colitis Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
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- 239000000463 material Substances 0.000 description 2
- KBOPZPXVLCULAV-UHFFFAOYSA-N mesalamine Chemical class NC1=CC=C(O)C(C(O)=O)=C1 KBOPZPXVLCULAV-UHFFFAOYSA-N 0.000 description 2
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- 241000009794 Agaricomycetes Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 102100037850 Interferon gamma Human genes 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 230000033540 T cell apoptotic process Effects 0.000 description 1
- 210000000068 Th17 cell Anatomy 0.000 description 1
- 241000042002 Trametes sanguinea Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 206010009887 colitis Diseases 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 238000003304 gavage Methods 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
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- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
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- 238000012549 training Methods 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a kind of pycnoporus samguineus extract and its extracting method and applications.Pycnoporus samguineus extract of the present invention is during the extraction process, using environmental-friendly water, ethyl alcohol as solvent, purifying is extracted using the mild means such as heating extraction, freeze-drying, have the advantages that simple process, production cost are low, avoid organic solvent pollution, and the extracting method can retain the activity of pycnoporus samguineus extract to greatest extent；It extracts obtained pycnoporus samguineus polyoses extract and ethanol extract can be used for preparing treatment inflammatory bowel medicine, special medical purposes food, functional food or health care product, both the inflammatory reaction of inflammatory bowel disease can have been inhibited, promote colonic tissue reparation simultaneously, has a good application prospect.
Description
Technical field:
The invention belongs to medicines and health protection fields, it is more particularly related to a kind of blood that can alleviate inflammatory bowel disease
Red samguineus extract and its extracting method and application.
Background technique:
Inflammatory bowel disease (Inflammatory bowel disease, IBD) is that a kind of chronic gut that the cause of disease is unknown is exempted from
Epidemic disease disease, including ulcerative colitis (Ulcerative Colitis, UC) and Crohn disease (Crohn ' s Disease,
DC).European and American areas is always the district occurred frequently of IBD；Although Asia, such as China, South Korea, Japan disease incidence it is lower,
It has in recent years been in the trend obviously risen.Disease incidence of the IBD in person between twenty and fifty is apparently higher than other age brackets crowd, and is often in
Breaking-out-alleviation-recurrence mode, therefore it is costly to cure the disease, or even is often required to cut off lesion by operation in the lesion later period
Position, physiology to patient and psychologically causes huge burden, seriously affects the quality of life of patient.The clinical treatment of IBD with
Based on glucocorticoid, 5-aminosalicylic acid class and immunosuppressor, furthermore to neutralize biological agent of the inflammatory factor as target
(such as Infliximab) also gradually rises in the treatment of IBD.However, the drug in these common schemes exists, apparent poison is secondary to be made
With, therefore how to be found from natural and synthesis compound and eradicate IBD drug, still shoulder heavy responsibilities.
Pycnoporus samguineus (Pycnoporus sanguineus, PC), also known as red bolt bacterium, Zhu blood bacterium etc., are to belong to mycota
(Kingdom Fungi), Basidiomycota (Basidiomycota), agaric guiding principle (Agaricomycetes), Aphyllophorales
(Polyporales), a kind of macro fungi of Polyporaceae (Polyporaceae).Past, due to wild blood red in nature
Samguineus resource scarcity brings larger difficulty for its exploitation；It is studied by a large amount of artificial culture, has developed the cultivation of section wood
The modes such as training, substituting stuff cultivation, liquid fermentation, bifurcation fermentation cultivation, obtain its resource more easy.At present for blood red close hole
The activity research of bacterium is less, and previously research finds that it has enhancing immune function, inhibits S 180 Growth on Mice, reduction is put
It treats, chemical therapy toxic side effect isoreactivity.There is treatment inflammation however, having not yet to see and obtaining by simple, environmentally friendly reagent
The correlative study of the pycnoporus samguineus extract of property enteropathy and report.
Summary of the invention:
The object of the present invention is to provide a kind of inflammatory reactions that can both inhibit inflammatory bowel disease, while promoting colonic tissue
The pycnoporus samguineus extract and its extracting method of reparation and application.
The first purpose of the invention is to provide pycnoporus samguineus polyoses extracts or pycnoporus samguineus ethanol extract to exist
The application in the drug, food or health care product of inflammatory bowel disease is treated in preparation.
The pycnoporus samguineus polyoses extract is after crushing pycnoporus samguineus fructification, with flooding, extracting solution
Concentrate is obtained after filtered concentrating filter liquor, concentrate uses ethanol precipitation again, and precipitating is taken to be dissolved in water, and so repeats to use second
Alcohol precipitates several times, and final precipitating is dissolved with water, then is dried to obtain pycnoporus samguineus polyoses extract.
It is further preferred that after crushing pycnoporus samguineus fructification, is impregnated with the water of 5-15 times of weight, reheat and extract,
It filters to get filtrate, repeats said extracted step several times, merging filtrate obtains concentrate after concentrating filter liquor, in concentrate
Ethyl alcohol is added, makes the volume fraction 70-90% of ethyl alcohol, stands, takes precipitating to be dissolved in water, make the volume of its volume and concentrate
It is equal, it repeats the above steps 2-4 times, final gained precipitating is dissolved in water, and is freeze-dried, obtains pycnoporus samguineus Polyose extraction
Object.
The pycnoporus samguineus ethanol extract is after crushing pycnoporus samguineus fructification, with alcohol steep, extraction
Liquid filters to get filtrate, and after filtrate concentration removal ethyl alcohol, obtains pycnoporus samguineus ethanol extract.
Further preferably, the pycnoporus samguineus ethanol extract is to crush pycnoporus samguineus fructification, add 30~
50 times of weight, volume fraction be that 60~90% ethanol waters carry out heating extraction, filtering, filter residue repeats the above steps 1~4
It is secondary, merge gained filtrate, is concentrated under reduced pressure into no ethanol flavor to get pycnoporus samguineus ethanol extract.
It is preferred that the volume fraction is the ethanol waters that 60~90% ethanol waters are volume fraction 86%.
Inflammatory bowel medicine, special medical purposes food, functional food or guarantor are treated the present invention also provides a kind of
Strong product, it includes above-mentioned pycnoporus samguineus polyoses extracts or pycnoporus samguineus ethanol extract as active constituent, main
Effect is the inflammatory reaction for including inhibition helper T lymphocyte, induces helper T lymphocyte apoptosis, promotes colonic tissue reparation, and control
Treat other systemic inflammatory reactions of inflammatory bowel disease.It further include medicine wherein when for treatment inflammatory bowel medicine
Acceptable carrier on.
Compared with the existing technology, the present invention has the advantage that
Pycnoporus samguineus extract of the present invention during the extraction process, using environmental-friendly water, ethyl alcohol as solvent, using heating
The mild means such as extraction, freeze-drying extract purifying, and with simple process, production cost is low, avoids organic solvent pollution
The advantages of, and the extracting method can retain the activity of pycnoporus samguineus extract to greatest extent；Extract obtained blood red close hole
Granulose extract and ethanol extract can be used for preparing treatment inflammatory bowel medicine, special medical purposes food, functionality
Food or health care product, can both inhibit the inflammatory reaction of inflammatory bowel disease, while promote colonic tissue reparation, have good answer
Use prospect.
Detailed description of the invention
With reference to the accompanying drawings and detailed description, to pycnoporus samguineus extract of the present invention and its extracting method and application
And beneficial effect is described in detail.
Fig. 1 is that 5 groups of animals colon lengths of experimental example of the present invention compare figure.
Fig. 2 is that 5 groups of animals colitis situation Macroscopic score of experimental example of the present invention compares figure.
Fig. 3 is that figure is compared in 5 groups of animals colon HE of experimental example dyeing of the present invention.
Fig. 4 is that figure is compared in 5 groups of animals serum LPS of experimental example measurement of the present invention.
Fig. 5 is T cell ratiometric result in 5 groups of animals colonic tissue of experimental example of the present invention.
Fig. 6 is Th cell proportion result in 5 groups of animals colonic tissue of experimental example of the present invention.
Fig. 7 is Treg cell proportion result in 5 groups of animals colonic tissue of the embodiment of the present invention.
Fig. 8 is Th1 cell proportion result in 5 groups of animals colonic tissue of the embodiment of the present invention.
Fig. 9 is Th2 cell proportion result in 5 groups of animals colonic tissue of the embodiment of the present invention.
Figure 10 is Th17 cell proportion result in 5 groups of animals colonic tissue of the embodiment of the present invention.
Specific embodiment
In order to be more clear the purpose of the present invention, technical solution and advantageous effects, with reference to embodiments, to this
Invention is further elaborated.It should be understood that embodiment described in this specification is just for the sake of this hair of explanation
It is bright, be not intended to limit the present invention, parameter, ratio of embodiment etc. can adaptation to local conditions make a choice and substance had no to result
It influences.
Unless stated otherwise, reagent used in the embodiment of the present invention, method and apparatus are the art conventional reagent, side
Method and equipment.
The preparation of 1 pycnoporus samguineus polyoses extract of embodiment
(1) pycnoporus samguineus fructification is taken, beats powder (25000r/min, 5s) through pulverizer, the use of aperture is 4 mesh (65 μm)
~50 mesh (355 μm) pharmacopeia test sieve is screened, and collection permeate (it can be by 4 meshes, but the powder of 50 meshes cannot be passed through
Mince, similarly hereinafter), it is added in 5 times of weight waters and carries out immersion 4h, heating extraction 2h, filtering collect filtrate；Repeat the above steps 2
It is secondary, merge gained filtrate, 60 DEG C are concentrated under reduced pressure into 2 times of pycnoporus samguineus fructification powder weight, obtain concentrate；
(2) dehydrated alcohol is added in the concentrate obtained by step (1), makes the volume fraction 70% of dehydrated alcohol, stand
Overnight, it takes precipitating to be dissolved in water, keeps its volume equal with the volume of concentrate, repeat the above steps 3 times, final gained precipitating adds
Suitable quantity of water dissolution, freeze-drying obtain pycnoporus samguineus polyoses extract.
The preparation of 2 pycnoporus samguineus polyoses extract of embodiment
(1) pycnoporus samguineus fructification is taken, powder (25000r/min, 5s) is beaten in pulverizer, the use of aperture is 4 mesh (65 μ
M)~50 mesh (355 μm) pharmacopeia test sieve is screened, and collects permeate；15 times of weight waters are added to carry out immersion 4h, heating extraction
2h, filtering collect filtrate；It repeats the above steps 1 time, merges gained filtrate, 60 DEG C are concentrated under reduced pressure into pycnoporus samguineus fructification
1 times of powder weight, obtains concentrate；
(2) dehydrated alcohol is added in the concentrate obtained by step (1), makes the volume fraction 80% of dehydrated alcohol, stand
Overnight, it takes precipitating to be dissolved in water, keeps its volume equal with the volume of concentrate, repeat the above steps 2 times, final gained precipitating adds
Suitable quantity of water dissolution, freeze-drying obtain pycnoporus samguineus polyoses extract.
The preparation of 3 pycnoporus samguineus ethanol extract of embodiment
Pycnoporus samguineus fructification is taken, powder (25000r/min, 5s) is beaten in pulverizer, the use of aperture is 4 mesh (65 μm)
~50 mesh (355 μm) pharmacopeia test sieve is screened, and permeate is collected, and the ethyl alcohol for being added to 30 times of bulking value scores 60% is water-soluble
In liquid, 80 DEG C are heated to reflux 2h, filtering, collect filtrate, and filter residue repeats above-mentioned be heated to reflux and operates 2 times, 3 filtrates of gained are closed
And 50 DEG C are concentrated under reduced pressure into no ethanol flavor, are freeze-dried to get pycnoporus samguineus ethyl alcohol extract.
The preparation of 4 pycnoporus samguineus ethanol extract of embodiment
Pycnoporus samguineus fructification is taken, powder (25000r/min, 5s) is beaten in pulverizer, the use of aperture is 4 mesh (65 μm)
~50 mesh (355 μm) pharmacopeia test sieve is screened, and permeate is collected, and the ethyl alcohol for being added to 50 times of bulking value scores 86% is water-soluble
In liquid, 80 DEG C are heated to reflux 1.5h, filtering, collect filtrate, and filter residue repeats above-mentioned be heated to reflux and operates 1 time, gained 2 times are filtered
Liquid merges, and 50 DEG C are concentrated under reduced pressure into no ethanol flavor, is freeze-dried to get pycnoporus samguineus ethyl alcohol extract.
The treatment for the inflammatory bowel disease mouse model that 5 pycnoporus samguineus extract of experimental example induces dextran sulfate
1, materials and methods
(1) modeling and administration
Balb/c male mice is taken, after adaptive feeding 3 days, is randomly divided into Normal group (12, N), mould by weight
Type group (12, M), (prepared by embodiment 1 for mesalazine group (MSLQ, 400mg/kg, 12), pycnoporus samguineus polyoses extract
Pycnoporus samguineus polyoses extract) high (PH), in (PM), low (PL) dosage group (400mg/kg, 200mg/kg, 100mg/
Kg, each 12), pycnoporus samguineus ethanol extract (pycnoporus samguineus ethanol extract prepared by embodiment 4) high (EH), in
(EM), low (EL) dosage group (400mg/kg, 200mg/kg, 100mg/kg, each 12), each medicine group continuous gavage 14 days, just
Normal group, model group give isometric pure water.After administration 7 days, except for the normal group, each group drinking water is changed to 3%DSS solution, together
When be administered, continue 7 days.
(2) materials and detection
During experiment, weight is measured and recorded daily, and mouse cerebration situation, pellet morphology, blood are observed during modeling
Just.Last dose for 24 hours after, orbital venous plexus takes blood, separates serum, and ELISA kit analyzes lipopolysaccharides
(Lipopolysaccharides, LPS) concentration.Take colon, amount length is simultaneously taken pictures, observation colon whether there is or not oedema, adhesion, burst
After the pathological changes such as ulcer, necrosis, its Macroscopic score (Macroscopic score) is recorded and calculated.Segmental colonic carries out bush
Essence-eosin stains (hematoxylin-eosin staining, HE) observes tissue change.Segmental colonic tissue separately is taken, is mentioned
Take lymphocyte, flow cytometry wherein T cell (CD3+), helper T lymphocyte (Th, CD3+CD4+), Th1 cell (CD3+
CD4+IFN-γ+), Th2 cell (CD3+CD4+IL-4+)、Th17(CD3+CD4+IL-17+), regulatory T cells (Treg, CD3+CD25 +Foxp3+) ratio variation.Wherein compared with N group, " * " " * * " respectively indicates p < 0.05, p < 0.01；Compared with M group, " # "
" ## " respectively indicates p < 0.05, p < 0.01
(3) result
Colon lengths (Fig. 1), macroscopical four item ratings (Fig. 2) and tissue HE dye (Fig. 3) and show compared with model group, blood
Red samguineus polyoses extract and ethanol extract can be obviously prolonged colon lengths, reduce Macroscopic score, while two kinds of extractions
Object can obviously inhibit tissue damage caused by inflammatory reaction, such as colon fluff structures to be repaired, and illustrate above two mention
It takes object and can be relieved the inflammatory bowel disease symptoms as caused by DSS.ELISA testing result shows (Fig. 4) that pycnoporus samguineus polysaccharide mentions
The content of LPS in serum can be substantially reduced by taking object and pycnoporus samguineus ethanol extract, further illustrate that two kinds of extracts are equal
It can promote colonic tissue reparation, restore intestinal barrier integrity.Colonic lymph cell T cell analysis is found (Fig. 5), it is blood red close
In obliqua polysaccharide extract, low dose group, the T cell ratio of pycnoporus samguineus ethanol extract low dose group is significantly lower than model
Group, and other drugs group has downward trend；Wherein, pycnoporus samguineus polyoses extract, ethanol extract can significantly reduce it
Middle Th cell proportion (Fig. 6).Further analysis finds, pycnoporus samguineus polyoses extract, pycnoporus samguineus ethanol extract pair
Regulatory T cells (Treg) and all types of cause inflammatory Th cell (including Th1, Th2, Th17) ratios have downward to act on (Fig. 7-
10).The above results show that pycnoporus samguineus extract can prevent and treat inflammatory bowel by adjusting body's immunity
Disease.
According to the disclosure and teachings of the above specification, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula carries out change and modification appropriate.Therefore, the invention is not limited to the specific embodiments disclosed and described above, to this
Some modifications and changes of invention should also be as falling into the scope of the claims of the present invention.In addition, although this specification
In use some specific terms, these terms are merely for convenience of description, does not limit the present invention in any way.
Claims (10)
1. a kind of preparation method of pycnoporus samguineus polyoses extract, which is characterized in that be to crush pycnoporus samguineus fructification
Afterwards, with flooding, obtain concentrate after the filtered concentrating filter liquor of extracting solution, concentrate uses ethanol precipitation again, take precipitating plus
Water dissolution, so repeats with ethanol precipitation several times, final precipitating is dissolved with water, then is dried to obtain pycnoporus samguineus polysaccharide and mentioned
Take object.
2. preparation method according to claim 1, which is characterized in that be after crushing pycnoporus samguineus fructification, to use 5-
The water of 15 times of weight impregnates, and reheats and extracts, filters to get filtrate, and repeats said extracted step several times, merging filtrate, filtrate warp
Concentrate is obtained after concentration, ethyl alcohol is added in concentrate, makes the volume fraction 70-90% of ethyl alcohol, is stood, and precipitating plus water are taken
Dissolution, keeps its volume equal with the volume of concentrate, repeats the above steps 2-4 times, and final gained precipitating is dissolved in water, and freezing is dry
It is dry, obtain pycnoporus samguineus polyoses extract.
3. a kind of pycnoporus samguineus polyoses extract that preparation method according to claim 1 or 2 is prepared.
4. a kind of preparation method of pycnoporus samguineus ethanol extract, which is characterized in that be to crush pycnoporus samguineus fructification
Afterwards, with alcohol steep, leaching liquor filters to get filtrate, and after filtrate concentration removal ethyl alcohol, obtains pycnoporus samguineus ethanol extract.
5. the preparation method according to claim 4, which is characterized in that the pycnoporus samguineus ethanol extract is by blood
Red samguineus fructification crushes, add 30~50 times of weight, volume fraction be 60~90% ethanol waters carry out heating extraction,
Filtering, filter residue repeat the above steps 1~4 time, merge gained filtrate, are concentrated under reduced pressure into no ethanol flavor to get pycnoporus samguineus second
Alcohol extracting thing.
6. preparation method according to claim 5, which is characterized in that the volume fraction is that 60~90% ethyl alcohol are water-soluble
Liquid is the ethanol water of volume fraction 86%.
7. a kind of pycnoporus samguineus ethanol extract being prepared according to preparation method described in claim 4,5 or 6.
8. drug, the food of pycnoporus samguineus polyoses extract or pycnoporus samguineus ethanol extract in preparation treatment inflammatory bowel disease
Application in product or health care product.
9. application according to claim 8, which is characterized in that the pycnoporus samguineus polyoses extract is claim
Pycnoporus samguineus polyoses extract described in 3, the pycnoporus samguineus ethanol extract are as claimed in claim 7 blood red close
Pore fungi ethanol extract.
10. a kind of drug for treating inflammatory bowel disease, food or health care product, which is characterized in that it contains as claimed in claim 3
Pycnoporus samguineus polyoses extract or pycnoporus samguineus ethanol extract as claimed in claim 7 are as active constituent.
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| CN110447457A (en) * | 2019-09-18 | 2019-11-15 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of pycnoporus samguineus new strains and its artificial cultivation method and purposes |
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