CN109645212A - A kind of preparation method of sesame protein - Google Patents
A kind of preparation method of sesame protein Download PDFInfo
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- CN109645212A CN109645212A CN201910058563.9A CN201910058563A CN109645212A CN 109645212 A CN109645212 A CN 109645212A CN 201910058563 A CN201910058563 A CN 201910058563A CN 109645212 A CN109645212 A CN 109645212A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
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Abstract
The invention discloses a kind of preparation method of sesame protein, include the following steps: sesame powder raw material and water mixing obtained sesame virgin pulp liquid, and once-combined enzyme enzymatic hydrolysis is added, obtains primary enzymolysis liquid；It adjusts primary enzymolysis liquid pH and carries out the heavy processing of acid, obtain enzymolysis protein powder；Sodium hydroxide solution is added into enzymolysis protein powder to adjust its pH and be completely dissolved, obtains albumen and redissolves liquid；Albumen redissolution liquid is successively subjected to ultrafiltration and reverse osmosis concentration processing, obtains protein concentrate；Protein concentrate and water are mixed by a certain percentage, protein slurry is made, secondary complex enzyme is added and is digested, and be centrifuged to obtain albumen precipitation, washing albumen precipitation pH to neutrality is to get arriving purifying protein powder；Purifying protein powder is subjected to freeze-drying process to get sesame protein.The preparation method of sesame protein provided by the invention, process safety, prepare it is convenient.
Description
Technical field
The present invention relates to sesame protein technical fields, and in particular to a kind of preparation method of sesame protein.
Background technique
Sesame is a kind of crops full of nutrition, is rich in grease and protein.Traditional sesame oil making technique is first to adopt
Seed is fried with high temperature, then produces Oleum Sesami with water substitution or high temperature squeezing method again.High temperature roasts that generate sesame oil strong
Fragrance, but oil colours pool is deepened, poor transparency;Protein serious degenerative in sesame residues and sesame-send cake, can only answer as feed
With resulting in waste of resources.Contain sulfur-containing amino acid abundant in sesame protein, is free of trypsin inhibitor, can be used as baby
Protein sources in youngster and wean baby food.
Summary of the invention
Shortcoming present in view of the above technology the present invention provides a kind of process safety, prepares convenient, sesame egg
The preparation method of the high sesame protein of white recovery rate.
The technical solution adopted by the present invention to solve the technical problems is: a kind of preparation method of sesame protein, including such as
Lower step: Step 1: the preparation of sesame powder raw material；Step 2: enzymatic hydrolysis: sesame powder raw material and water being mixed, sesame magma is made
Liquid adjusts sesame virgin pulp liquid pH, and once-combined enzyme enzymatic hydrolysis is added, and destroy the enzyme treatment is carried out to the end of enzyme digestion reaction, is obtained primary
Enzymolysis liquid；Step 3: acid is heavy: adjusting primary enzymolysis liquid pH and carry out the heavy processing of acid, obtain enzymolysis protein powder；Step 4: redissolving:
Sodium hydroxide solution is added into enzymolysis protein powder and adjusts its pH, and enzymolysis protein powder is completely dissolved, obtains albumen and redissolves liquid；
Step 5: ultrafiltration and concentration: albumen redissolution liquid successively being carried out ultrafiltration and reverse osmosis concentration is handled, obtains protein concentrate；Step
Six, it purifies: protein concentrate and water being mixed by a certain percentage, protein slurry is made, regulatory protein slurries pH is simultaneously added secondary compound
Enzyme carries out ultrasonic wave added enzymatic hydrolysis and enzymolysis liquid is carried out destroy the enzyme treatment after digesting, and is centrifuged to obtain albumen precipitation, washes
Albumen precipitation pH arrives purifying protein powder to neutrality；Step 7: freeze-drying: purifying protein powder is carried out at freeze-drying
Reason is to get sesame protein.
Preferably, in the step 1 sesame powder raw material preparation process are as follows: will be after sesame decladding using the leaching of No. six solvents
Bubble obtains the sesame seed meal of degreasing after precipitation, and resid amount is 1.4~1.8%, and the sesame seed meal of degreasing is pulverized and sieved to obtain
Grain size is the sesame powder raw material of 100~150 meshes.
Preferably, the process digested in the step 2 is as follows: by sesame powder raw material and water by solid-liquid ratio 1g:10~15mL
It mixes and sesame virgin pulp liquid is made, hydrochloric acid or citric acid are added in sesame virgin pulp liquid by pH and is adjusted to 4~5, and is added once-combined
Enzyme enzymatic hydrolysis, enzymolysis temperature are 50~55 DEG C, and the enzyme digestion reaction time is 3~5 hours, to the end of enzyme digestion reaction, are warming up to 85
~95 DEG C enzyme deactivation 10~20 minutes, obtain primary enzymolysis liquid.
Preferably, the once-combined enzyme in the step 2 is protease+cellulase+carbohydrase+pectase, the egg
White enzyme: cellulase: carbohydrase: the usage ratio of pectase is 1:2~4:1~2:4~6, and the enzyme concentration of once-combined enzyme
For 1200~1800u/g of black sesame powder raw material dosage.
Preferably, the heavy process of acid is as follows in the step 3: it adjusts primary enzymolysis liquid pH to 4~5 and carries out the heavy processing of acid,
The heavy temperature of acid is 40~45 DEG C, through being centrifugally separating to obtain enzymolysis protein powder after floccule to appear.
Preferably, the process redissolved in the step 4 is as follows: sodium hydroxide solution being added into enzymolysis protein powder and adjusts
PH to 8~9, and be completely dissolved enzymolysis protein powder at being 25~35 DEG C in temperature, it obtains albumen and redissolves liquid.
Preferably, hyperfiltration treatment uses the aperture of ultrafiltration membrane for 20~30 nanometers in the step 5, ultrafiltration pressure 0.3
~0.5MPa, ultrafiltrate temperature are 35~45 DEG C.
Preferably, reverse osmosis concentration treatment conditions are as follows in the step 5: pressure be 0.3~0.5MPa, pH be 7.5~
8.5, temperature is 15~25 DEG C.
Preferably, the process purified in the step 6 is as follows: protein concentrate and water are mixed by solid-liquid ratio 1g:5~10mL
Even obtained protein slurry, regulatory protein slurries pH to 5~6, and secondary complex enzyme is added and carries out ultrasonic wave added enzymatic hydrolysis, wait digest
Afterwards, enzymolysis liquid is heated to 85~95 DEG C of destroy the enzyme treatments 10~20 minutes, is centrifuged to obtain albumen precipitation, using 30~40
DEG C distilled water wash albumen precipitation pH to neutrality to get arrive purifying protein powder.
Preferably, the secondary complex enzyme is hemicellulase+zytase+pectinesterase hydrolase, the hemicellulose
Plain enzyme: zytase: the usage ratio of pectinesterase hydrolase is 1:3~5:2~3, and the enzyme concentration of secondary complex enzyme is egg
1.6~2.0ug/g of white slurries dosage.
Compared with prior art, the present invention the beneficial effect is that: the preparation method of sesame protein provided by the invention passes through
It is digested using the once-combined enzyme being made of protease+cellulase+carbohydrase+pectase, can effectively destroy black sesame powder
The cell wall of cell can effectively improve the recovery rate of sesame protein so that intracellular albumen is easier to extract；By successively successively
It is redissolved, ultrafiltration, concentration, impurity other in sesame protein can be efficiently separated, to improve the purity of sesame protein；
By being digested in purifying using the secondary complex enzyme being made of hemicellulase+zytase+pectinesterase hydrolase,
The false albumen such as some carbohydrates can be effectively removed, to increase the recovery rate and purity of sesame protein simultaneously；The preparation of the sesame protein
Method, process safety prepare convenient, and the recovery rate of sesame protein is up to 70% or more, the sesame protein purity extracted is reachable
90.0% or more.
Specific embodiment
The present invention is described in further detail below, to enable those skilled in the art's refer to the instruction text being capable of evidence
To implement.
The present invention provides a kind of preparation methods of sesame protein, include the following steps:
Step 1: No. six solvent soakings will be used after sesame decladding, the sesame seed meal of degreasing, resid amount 1.4 are obtained after precipitation
~1.8%, and the sesame seed meal of degreasing is pulverized and sieved to obtain the sesame powder raw material that granular size is 100~150 meshes；
Sesame virgin pulp liquid is made Step 2: sesame powder raw material and water are mixed by solid-liquid ratio 1g:10~15mL, in sesame virgin pulp liquid
PH is adjusted to 4~5 by middle addition hydrochloric acid, and once-combined enzyme enzymatic hydrolysis is added, the once-combined enzyme be protease+cellulase+
Carbohydrase+pectase, the protease: cellulase: carbohydrase: the usage ratio of pectase is 1:2~4:1~2:4~6,
And the enzyme concentration of once-combined enzyme is 1200~1800u/g of black sesame powder raw material dosage, enzymolysis temperature is 40~45 DEG C, enzyme
Solving the reaction time is 4~6 hours, to the end of enzyme digestion reaction, is warming up to 85~95 DEG C of enzyme deactivations 10~20 minutes, obtains primary enzymolysis
Liquid；
Step 3: adjusting primary enzymolysis liquid pH to 4~5 carries out the heavy processing of acid, the heavy temperature of acid is 40~45 DEG C, floccule to appear
By being centrifugally separating to obtain enzymolysis protein powder；
Step 4: sodium hydroxide solution is added into enzymolysis protein powder adjusts pH to 8~9, and incited somebody to action at being 25~35 DEG C in temperature
Enzymolysis protein powder is completely dissolved, and is obtained albumen and is redissolved liquid；
Step 5: albumen redissolution liquid is successively carried out ultrafiltration and reverse osmosis concentration processing, protein concentrate is obtained；
Wherein, the hyperfiltration treatment uses the aperture of ultrafiltration membrane for 20~30 nanometers, and ultrafiltration pressure is 0.3~0.5MPa, ultrafiltration
Temperature is 35~45 DEG C；
Wherein, the reverse osmosis concentration treatment conditions are as follows: pressure be 0.3~0.5MPa, pH be 7.5~8.5, temperature be 15~
25℃；
Protein slurry, regulatory protein slurries pH to 5 is made Step 6: protein concentrate and water are mixed by solid-liquid ratio 1g:5~10mL
~6, and secondary complex enzyme is added and carries out ultrasonic wave added enzymatic hydrolysis, the secondary complex enzyme is hemicellulase+zytase+pectin
Methyl esters hydrolase, the hemicellulase: zytase: the usage ratio of pectinesterase hydrolase is 1:3~5:2~3, and two
The enzyme concentration of secondary complex enzyme is that enzymolysis liquid is heated to 85~95 DEG C after digesting by 1.6~2.0ug/g of protein slurry dosage
It destroy the enzyme treatment 10~20 minutes, is centrifuged to obtain albumen precipitation, washes albumen precipitation pH using 30~40 DEG C of distilled water
To neutrality to get arrive purifying protein powder；
Step 7: purifying protein powder is carried out freeze-drying process to get sesame protein.
Embodiment 1
No. six solvent soakings will be used after sesame decladding, obtain the sesame seed meal of degreasing, resid amount 1.4% after precipitation, and will take off
The sesame seed meal of rouge pulverizes and sieves to obtain the sesame powder raw material that granular size is 100 meshes；Sesame powder raw material and water are pressed into solid-liquid ratio
1g:10mL, which is mixed, is made sesame virgin pulp liquid, and hydrochloric acid or citric acid are added in sesame virgin pulp liquid by pH and is adjusted to 4, and is added primary
Complex enzyme zymohydrolysis, the once-combined enzyme be protease+cellulase+carbohydrase+pectase, the protease: cellulase:
Carbohydrase: the usage ratio of pectase is 1:2:1:4, and the enzyme concentration of once-combined enzyme is the 1200u/ of black sesame powder raw material dosage
G, enzymolysis temperature are 40 DEG C, and the enzyme digestion reaction time is 4 hours, to the end of enzyme digestion reaction, are warming up to 85 DEG C of enzyme deactivations 10 minutes,
Obtain primary enzymolysis liquid；It adjusts primary enzymolysis liquid pH to 4 and carries out the heavy processing of acid, the heavy temperature of acid is 40 DEG C, after floccule to appear
It is centrifuged to obtain enzymolysis protein powder；Sodium hydroxide solution is added into enzymolysis protein powder and adjusts pH to 8, and is 25 in temperature
Enzymolysis protein powder is completely dissolved at DEG C, albumen is obtained and redissolves liquid；Albumen redissolution liquid is successively subjected to ultrafiltration and reverse osmosis concentration
Processing, obtains protein concentrate；Wherein, the hyperfiltration treatment uses the aperture of ultrafiltration membrane for 20 nanometers, ultrafiltration pressure 0.3MPa,
Ultrafiltrate temperature is 35 DEG C；Wherein, the reverse osmosis concentration treatment conditions are as follows: pressure 0.3MPa, pH 7.5, temperature 15
℃；Protein slurry is made Step 6: protein concentrate and water are mixed by solid-liquid ratio 1g:5mL, regulatory protein slurries pH to 5, and add
Enter secondary complex enzyme and carry out ultrasonic wave added enzymatic hydrolysis, the secondary complex enzyme is hemicellulase+zytase+pectinesterase hydrolysis
Enzyme, the hemicellulase: zytase: the usage ratio of pectinesterase hydrolase is 1:3:2, and secondary complex enzyme is enzyme
Amount is that enzymolysis liquid is heated to 85 DEG C of destroy the enzyme treatments 10 minutes after digesting by the 1.6ug/g of protein slurry dosage, through centrifugation point
From albumen precipitation is obtained, purifying protein powder is arrived to neutrality using 30 DEG C of distilled water washing albumen precipitation pH；Egg will be purified
White powder carries out freeze-drying process to get sesame protein.
Embodiment 2
No. six solvent soakings will be used after sesame decladding, obtain the sesame seed meal of degreasing, resid amount 1.8% after precipitation, and will take off
The sesame seed meal of rouge pulverizes and sieves to obtain the sesame powder raw material that granular size is 150 meshes；Sesame powder raw material and water are pressed into solid-liquid ratio
1g:15mL, which is mixed, is made sesame virgin pulp liquid, and hydrochloric acid or citric acid are added in sesame virgin pulp liquid by pH and is adjusted to 5, and is added primary
Complex enzyme zymohydrolysis, the once-combined enzyme be protease+cellulase+carbohydrase+pectase, the protease: cellulase:
Carbohydrase: the usage ratio of pectase is 1:4:2:6, and the enzyme concentration of once-combined enzyme is the 1800u/ of black sesame powder raw material dosage
G, enzymolysis temperature are 45 DEG C, and the enzyme digestion reaction time is 6 hours, to the end of enzyme digestion reaction, are warming up to 95 DEG C of enzyme deactivations 20 minutes,
Obtain primary enzymolysis liquid；It adjusts primary enzymolysis liquid pH to 5 and carries out the heavy processing of acid, the heavy temperature of acid is 45 DEG C, after floccule to appear
It is centrifuged to obtain enzymolysis protein powder；Sodium hydroxide solution is added into enzymolysis protein powder and adjusts pH to 9, and is 35 in temperature
Enzymolysis protein powder is completely dissolved at DEG C, albumen is obtained and redissolves liquid；Albumen redissolution liquid is successively subjected to ultrafiltration and reverse osmosis concentration
Processing, obtains protein concentrate；Wherein, the hyperfiltration treatment uses the aperture of ultrafiltration membrane for 30 nanometers, ultrafiltration pressure 0.5MPa,
Ultrafiltrate temperature is 45 DEG C；Wherein, the reverse osmosis concentration treatment conditions are as follows: pressure 0.5MPa, pH 8.5, temperature 25
℃；Protein slurry is made Step 6: protein concentrate and water are mixed by solid-liquid ratio 1g:10mL, regulatory protein slurries pH to 6, and
Secondary complex enzyme is added and carries out ultrasonic wave added enzymatic hydrolysis, the secondary complex enzyme is hemicellulase+zytase+pectinesterase water
Solve enzyme, the hemicellulase: zytase: the usage ratio of pectinesterase hydrolase be 1:5:3, and secondary complex enzyme plus
Enzyme amount is that enzymolysis liquid is heated to 95 DEG C of destroy the enzyme treatments 20 minutes, through being centrifuged after digesting by the 2.0ug/g of protein slurry dosage
Isolated albumen precipitation arrives purifying protein powder to neutrality using 40 DEG C of distilled water washing albumen precipitation pH；It will purifying
Albumen powder carries out freeze-drying process to get sesame protein.
Embodiment 3
No. six solvent soakings will be used after sesame decladding, obtain the sesame seed meal of degreasing, resid amount 1.6% after precipitation, and will take off
The sesame seed meal of rouge pulverizes and sieves to obtain the sesame powder raw material that granular size is 125 meshes；Sesame powder raw material and water are pressed into solid-liquid ratio
1g:12mL, which is mixed, is made sesame virgin pulp liquid, and hydrochloric acid or citric acid are added in sesame virgin pulp liquid by pH and is adjusted to 4.5, and is added one
Secondary complex enzyme zymohydrolysis, the once-combined enzyme are protease+cellulase+carbohydrase+pectase, the protease: cellulose
Enzyme: carbohydrase: the usage ratio of pectase is 1:3:1.5:5, and the enzyme concentration of once-combined enzyme is black sesame powder raw material dosage
1500u/g, enzymolysis temperature are 42 DEG C, and the enzyme digestion reaction time is 5 hours, to the end of enzyme digestion reaction, are warming up to 90 DEG C of enzyme deactivations
15 minutes, obtain primary enzymolysis liquid；It adjusts primary enzymolysis liquid pH to 4.5 and carries out the heavy processing of acid, the heavy temperature of acid is 42 DEG C, to appear
Through being centrifugally separating to obtain enzymolysis protein powder after floccule；Sodium hydroxide solution is added into enzymolysis protein powder and adjusts pH to 8.5, and
Enzymolysis protein powder is completely dissolved at being 30 DEG C in temperature, albumen is obtained and redissolves liquid；By albumen redissolution liquid successively carry out ultrafiltration and
Reverse osmosis concentration processing, obtains protein concentrate；Wherein, the hyperfiltration treatment uses the aperture of ultrafiltration membrane for 25 nanometers, ultrafiltration pressure
Power is 0.4MPa, and ultrafiltrate temperature is 40 DEG C；Wherein, the reverse osmosis concentration treatment conditions are as follows: pressure 0.4MPa, pH 8,
Temperature is 20 DEG C；Protein slurry, regulatory protein slurries pH is made Step 6: protein concentrate and water are mixed by solid-liquid ratio 1g:8mL
To 5.5, and secondary complex enzyme is added and carries out ultrasonic wave added enzymatic hydrolysis, the secondary complex enzyme is hemicellulase+zytase+fruit
Glue methyl esters hydrolase, the hemicellulase: zytase: the usage ratio of pectinesterase hydrolase is 1:4:2.5, and secondary
The enzyme concentration of complex enzyme is that enzymolysis liquid is heated to 90 DEG C of destroy the enzyme treatments 15 and divided by the 1.8ug/g of protein slurry dosage after digesting
Clock is centrifuged to obtain albumen precipitation, arrives purifying protein to neutrality using 35 DEG C of distilled water washing albumen precipitation pH
Powder；Purifying protein powder is subjected to freeze-drying process to get sesame protein.
Although the embodiments of the present invention have been disclosed as above, but it is not limited in listed fortune in specification and embodiments
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily real
Now other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is not limited to
Specific details.
Claims (10)
1. a kind of preparation method of sesame protein, which comprises the steps of:
Step 1: the preparation of sesame powder raw material；
Step 2: enzymatic hydrolysis: sesame powder raw material and water being mixed, sesame virgin pulp liquid is made, adjust sesame virgin pulp liquid pH, and be added one
Secondary complex enzyme zymohydrolysis carries out destroy the enzyme treatment to the end of enzyme digestion reaction, obtains primary enzymolysis liquid；
Step 3: acid is heavy: adjusting primary enzymolysis liquid pH and carry out the heavy processing of acid, obtain enzymolysis protein powder；
Step 4: redissolving: sodium hydroxide solution is added into enzymolysis protein powder and adjusts its pH, and enzymolysis protein powder is completely molten
Solution obtains albumen and redissolves liquid；
Step 5: ultrafiltration and concentration: albumen redissolution liquid successively being carried out ultrafiltration and reverse osmosis concentration is handled, obtains protein concentrate；
Step 6: purifying: protein concentrate and water being mixed by a certain percentage, protein slurry is made, regulatory protein slurries pH is simultaneously added
Secondary complex enzyme carries out ultrasonic wave added enzymatic hydrolysis and enzymolysis liquid is carried out destroy the enzyme treatment after digesting, and it is heavy to be centrifuged to obtain albumen
Form sediment, washing albumen precipitation pH to neutrality to get arrive purifying protein powder；
Step 7: freeze-drying: purifying protein powder is carried out freeze-drying process to get sesame protein.
2. the preparation method of sesame protein as described in claim 1, which is characterized in that sesame powder raw material in the step 1
Preparation process are as follows: No. six solvent soakings will be used after sesame decladding, obtain the sesame seed meal of degreasing, resid amount 1.4 after precipitation
~1.8%, and the sesame seed meal of degreasing is pulverized and sieved to obtain the sesame powder raw material that granular size is 100~150 meshes.
3. the preparation method of sesame protein as described in claim 1, which is characterized in that the process digested in the step 2 is such as
Under: sesame powder raw material and water are mixed by solid-liquid ratio 1g:10~15mL, sesame virgin pulp liquid is made, salt is added in sesame virgin pulp liquid
PH is adjusted to 4~5 by acid, and once-combined enzyme enzymatic hydrolysis is added, and enzymolysis temperature is 50~55 DEG C, and the enzyme digestion reaction time is 3~
5 hours, to the end of enzyme digestion reaction, 85~95 DEG C of enzyme deactivations 10~20 minutes is warming up to, primary enzymolysis liquid is obtained.
4. the preparation method of sesame protein as claimed in claim 3, which is characterized in that the once-combined enzyme in the step 2
For protease+cellulase+carbohydrase+pectase, the protease: cellulase: carbohydrase: the usage ratio of pectase is
1:2~4:1~2:4~6, and the enzyme concentration of once-combined enzyme is 1200~1800u/g of black sesame powder raw material dosage.
5. the preparation method of sesame protein as described in claim 1, which is characterized in that the process that acid sinks in the step 3 is such as
Under: it adjusts primary enzymolysis liquid pH to 4~5 and carries out the heavy processing of acid, the heavy temperature of acid is 40~45 DEG C, through being centrifuged after floccule to appear
Isolated enzymolysis protein powder.
6. the preparation method of sesame protein as described in claim 1, which is characterized in that the process redissolved in the step 4 is such as
Under: sodium hydroxide solution is added into enzymolysis protein powder and adjusts pH to 8~9, and by enzymolysis protein at being 25~35 DEG C in temperature
Powder is completely dissolved, and is obtained albumen and is redissolved liquid.
7. the preparation method of sesame protein as described in claim 1, which is characterized in that hyperfiltration treatment uses in the step 5
The aperture of ultrafiltration membrane is 20~30 nanometers, and ultrafiltration pressure is 0.3~0.5MPa, and ultrafiltrate temperature is 35~45 DEG C.
8. the preparation method of sesame protein as described in claim 1, which is characterized in that in the step 5 at reverse osmosis concentration
Manage bar part is as follows: pressure is 0.3~0.5MPa, and pH is 7.5~8.5, and temperature is 15~25 DEG C.
9. the preparation method of sesame protein as described in claim 1, which is characterized in that the process purified in the step 6 is such as
Under: protein concentrate and water are mixed by solid-liquid ratio 1g:5~10mL, protein slurry is made, regulatory protein slurries pH to 5~6, and add
Enter secondary complex enzyme progress ultrasonic wave added enzymatic hydrolysis and enzymolysis liquid is heated to 10~20 points of 85~95 DEG C of destroy the enzyme treatments after digesting
Clock is centrifuged to obtain albumen precipitation, arrives purifying to neutrality using 30~40 DEG C of distilled water washing albumen precipitation pH
Albumen powder.
10. the preparation method of sesame protein as claimed in claim 9, which is characterized in that the secondary complex enzyme is hemicellulose
Plain enzyme+zytase+pectinesterase hydrolase, the hemicellulase: zytase: the usage ratio of pectinesterase hydrolase
For 1:3~5:2~3, and the enzyme concentration of secondary complex enzyme is 1.6~2.0ug/g of protein slurry dosage.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114517221A (en) * | 2022-03-15 | 2022-05-20 | 济南大学 | Method for preparing sesame protein from sesame oil by-product by water extraction method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704462A (en) * | 2014-01-06 | 2014-04-09 | 湖北长坂雄风植物油有限公司 | Extraction and purification method for sesame proteins |
| CN104824334A (en) * | 2015-04-03 | 2015-08-12 | 安徽新桥工贸有限责任公司 | Method for supersonic wave-assistant extraction of sesame protein |
| CN105767455A (en) * | 2016-03-17 | 2016-07-20 | 武汉轻工大学 | Method for preparing hickory nut protein by enzyme method |
| CN106086133A (en) * | 2016-06-20 | 2016-11-09 | 武汉轻工大学 | A kind of enzyme process prepares the method for Semen coryli heterophyllae albumen |
| CN109055469A (en) * | 2018-08-29 | 2018-12-21 | 武汉轻工大学 | A kind of preparation method of pumpkin albumen |
-
2019
- 2019-01-22 CN CN201910058563.9A patent/CN109645212A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704462A (en) * | 2014-01-06 | 2014-04-09 | 湖北长坂雄风植物油有限公司 | Extraction and purification method for sesame proteins |
| CN104824334A (en) * | 2015-04-03 | 2015-08-12 | 安徽新桥工贸有限责任公司 | Method for supersonic wave-assistant extraction of sesame protein |
| CN105767455A (en) * | 2016-03-17 | 2016-07-20 | 武汉轻工大学 | Method for preparing hickory nut protein by enzyme method |
| CN106086133A (en) * | 2016-06-20 | 2016-11-09 | 武汉轻工大学 | A kind of enzyme process prepares the method for Semen coryli heterophyllae albumen |
| CN109055469A (en) * | 2018-08-29 | 2018-12-21 | 武汉轻工大学 | A kind of preparation method of pumpkin albumen |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114517221A (en) * | 2022-03-15 | 2022-05-20 | 济南大学 | Method for preparing sesame protein from sesame oil by-product by water extraction method |
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Application publication date: 20190419 |