CN109633932A - A kind of smaller green leaf hopper ovum observation device and smaller green leaf hopper ovum observation method - Google Patents

A kind of smaller green leaf hopper ovum observation device and smaller green leaf hopper ovum observation method Download PDF

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Publication number
CN109633932A
CN109633932A CN201910040693.XA CN201910040693A CN109633932A CN 109633932 A CN109633932 A CN 109633932A CN 201910040693 A CN201910040693 A CN 201910040693A CN 109633932 A CN109633932 A CN 109633932A
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China
Prior art keywords
green leaf
leaf hopper
smaller green
blue
ovum
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CN201910040693.XA
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Chinese (zh)
Inventor
王志博
肖强
刘关华
李鑫
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Tea Research Institute Chinese Academy of Agricultural Sciences
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Tea Research Institute Chinese Academy of Agricultural Sciences
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Priority to CN201910040693.XA priority Critical patent/CN109633932A/en
Publication of CN109633932A publication Critical patent/CN109633932A/en
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    • GPHYSICS
    • G02OPTICS
    • G02CSPECTACLES; SUNGLASSES OR GOGGLES INSOFAR AS THEY HAVE THE SAME FEATURES AS SPECTACLES; CONTACT LENSES
    • G02C11/00Non-optical adjuncts; Attachment thereof
    • G02C11/04Illuminating means
    • GPHYSICS
    • G02OPTICS
    • G02CSPECTACLES; SUNGLASSES OR GOGGLES INSOFAR AS THEY HAVE THE SAME FEATURES AS SPECTACLES; CONTACT LENSES
    • G02C3/00Special supporting arrangements for lens assemblies or monocles
    • G02C3/02Arrangements for supporting by headgear
    • GPHYSICS
    • G02OPTICS
    • G02CSPECTACLES; SUNGLASSES OR GOGGLES INSOFAR AS THEY HAVE THE SAME FEATURES AS SPECTACLES; CONTACT LENSES
    • G02C7/00Optical parts
    • G02C7/10Filters, e.g. for facilitating adaptation of the eyes to the dark; Sunglasses
    • GPHYSICS
    • G09EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09BEDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/36Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for zoology

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Ophthalmology & Optometry (AREA)
  • Optics & Photonics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Computational Mathematics (AREA)
  • Pure & Applied Mathematics (AREA)
  • Algebra (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mathematical Analysis (AREA)
  • Mathematical Optimization (AREA)
  • Mathematical Physics (AREA)
  • Zoology (AREA)
  • Business, Economics & Management (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Educational Administration (AREA)
  • Educational Technology (AREA)
  • Theoretical Computer Science (AREA)
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Abstract

A kind of smaller green leaf hopper ovum observation device and smaller green leaf hopper ovum observation method, belong to biology techniques field.The smaller green leaf hopper ovum observes the orange glasses that device includes fixing head band, connect with the blue-ray light of fixing head band connection and with blue-ray light, the blue-ray light includes battery case, the positive Middle face of the battery case is equipped with lamp cap, blue light source is equipped in the lamp cap, the hinged folding in battery case bottom, the folding bottom is equipped with one group of fixing clamp, and the fixing clamp is equipped with orange glasses.The present invention utilizes blue light irradiated Tea tender tip, in the case where wearing orange glasses, it can be seen that the tough subcutaneous smaller green leaf hopper ovum of tea branch generates green fluorescence.It compared with original Microanatomical Observation method, using the device of the invention, is not limited by anatomy, and can more quick, efficiently, accurately realize the purpose of the quantity of observation and statistics smaller green leaf hopper ovum.In addition, the device of the invention is light, portable, it can be achieved that night field work.

Description

A kind of smaller green leaf hopper ovum observation device and smaller green leaf hopper ovum observation method
Technical field
The present invention relates to biology techniques fields, and in particular to a kind of smaller green leaf hopper ovum observation device and smaller green leaf hopper ovum are seen Examine method.
Background technique
Tea lesser leafhopper is that China is distributed Pests of Tea-Plants that is most wide, endangering most serious at present.The worm is tender by piercing and sucking tea tree Tip juice is endangered, and young leaves is all shrivelled when serious falls off.In studying smaller green leaf hopper, it can usually observe and count its ovum Quantity assesses its spawning rate, population quantity, pesticide supplying effect etc..Since smaller green leaf hopper habit is female adult oviposition Device is pierced into tea tree tender tip, and ovum is produced in phloem tissue.Previous smaller green leaf hopper ovum observation statistical method is to be placed on tea branch Epidermis is peeled off under anatomical lens to find ovum.This method is firstly the need of skilled micromanipulative technique, and low efficiency, most It is important that being difficult to ensure the accuracy of statistical result.
Summary of the invention
In view of the problems of the existing technology, it is an object of the invention to design to provide a kind of smaller green leaf hopper ovum observation device And the technical solution of smaller green leaf hopper ovum observation method.The present invention is by being radiated at the tea branch with smaller green leaf hopper ovum for blue light On, when taking the observation of orange glasses, green fluorescence can be presented in the tough subcutaneous smaller green leaf hopper ovum of tea branch, can letter using the device List efficiently, accurately counts the quantity of ovum
A kind of smaller green leaf hopper ovum observes device, it is characterised in that the blue light including fixing head band and fixing head band connection Lamp and the orange glasses connecting with blue-ray light, the blue-ray light include battery case, and the positive Middle face of the battery case is set There is lamp cap, blue light source, the hinged folding in battery case bottom are equipped in the lamp cap, the folding bottom is equipped with one Group fixing clamp, the fixing clamp are equipped with orange glasses.
A kind of smaller green leaf hopper ovum observes device, it is characterised in that the fixing head band includes both ends and blue-ray light Fixed endless belt, the fixed horizontal stripes in endless belt middle part, the horizontal stripes other end and blue-ray light top are fixed.
A kind of smaller green leaf hopper ovum observes device, it is characterised in that the blue light source uses XP-E LED bulb As light source, laser emission wavelength is 445nm blue light.
A kind of smaller green leaf hopper ovum observes device, it is characterised in that the battery case side is controlled equipped with blue-ray light Switch.
A kind of smaller green leaf hopper ovum observes device, it is characterised in that the orange glasses include orange eyeglass, institute U-shaped wing of nose groove is equipped in the middle part of the orange eyeglass stated.
A kind of smaller green leaf hopper ovum observes device, it is characterised in that the blue-ray light both ends be equipped with for annular First buckle of band connection, the blue-ray light top are equipped with for buckling with the second of horizontal band connection.
A kind of smaller green leaf hopper ovum observes device, it is characterised in that the endless belt is equipped with for adjusting annular Adjusting buckle with length.
The observation method of a kind of smaller green leaf hopper ovum, it is characterised in that under dark condition, irradiated by blue light Smaller green leaf hopper ovum recycles orange eyeglass observation smaller green leaf hopper ovum.
The observation method, it is characterised in that specifically includes the following steps:
1) in the tender tea branch of tea place smaller green leaf hopper Onset acquisition 10cm long children;
2) it takes back tea stem cutting behind laboratory in long 10cm, the water suction flower mud of wide 5cm, high 4cm;
3) flower mud for being inserted with tea branch is taken in darkroom;
4) a kind of smaller green leaf hopper ovum observation device is worn to head, opens blue-ray light, light beam impinges upon on tea branch, passes through orange Color glasses observe the smaller green leaf hopper ovum of aobvious fluorescent green.
Advantageous effects of the invention: it in the inventive solutions, using blue light irradiated Tea tender tip, is wearing In the case where wearing orange glasses, it can be seen that the tough subcutaneous smaller green leaf hopper ovum of tea branch generates green fluorescence.With original microdissection Observation method is compared, and using the device of the invention, is not limited by anatomy, and can more quick, efficiently, accurately be realized The purpose of the quantity of observation and statistics smaller green leaf hopper ovum.In addition, the device of the invention is light, portable, it can be achieved that night field Operation.
Detailed description of the invention
Fig. 1 is experimental rig structural schematic diagram of the present invention;
Fig. 2 is the fixation header structure band schematic diagram of experimental rig of the present invention;
Fig. 3 is the blue-ray light structural schematic diagram of experimental rig of the present invention;
Fig. 4 is the orange Glasses structure schematic diagram of experimental rig of the present invention;
Fig. 5 is the effect that smaller green leaf hopper ovum is observed after wearing the upper device;
Fig. 6 is that anatomical lens is cooperated to use the effect of device observation smaller green leaf hopper ovum.
In figure: 1- fixing head band;The endless belt 101-;102- horizontal stripes;103- adjusts buckle;2- blue-ray light;201- battery Box;202- lamp cap;203- blue light signal controlled switch;204- first is buckled;205- folding;206- fixing clamp;207- second is buckled; 208- blue light source;The orange glasses of 3-;The orange eyeglass of 301-;302-U type wing of nose groove.
Specific embodiment
Below in conjunction with the attached drawing in embodiment of the present invention, the technical solution in embodiment of the present invention is carried out clear Clear, complete description.It may be noted that following embodiment is only a part of the embodiments of the present invention, and not all embodiment.
Embodiment 1: smaller green leaf hopper ovum observes device
As shown in Figs 1-4, smaller green leaf hopper ovum observation device include fixing head band 1, the blue-ray light being connect with fixing head band 12 and The orange glasses 3 being connect with blue-ray light 2.
Fixing head band 1 includes both ends and the endless belt 101 that blue-ray light 2 is fixed, and fixes horizontal stripes 102 in the middle part of endless belt 101, It is fixed at the top of 102 other end of horizontal stripes and blue-ray light 2, as preferred structure, 2 both ends of blue-ray light be equipped with for endless belt 101 The first of connection buckles 204, and the second buckle 207 for connecting with horizontal stripes 102 is equipped at the top of blue-ray light 2.101 He of endless belt Horizontal stripes 102 are fixed with blue-ray light 2 by the first buckle 204 and the second buckle 207, facilitate fixation.In order to facilitate endless belt 101 The adjusting of length, endless belt 101, which is equipped with, adjusts buckle 103.
Blue-ray light 2 includes battery case 201, and the positive Middle face of battery case 201 is equipped with lamp cap 202, is equipped with blue light in lamp cap 202 Light source 208.For blue light source 208 preferably using XP-E LED bulb as light source, laser emission wavelength is 445nm blue light.For Convenient for the control to blue light source 208, blue light signal controlled switch 203 is equipped in battery case 201 side.
The hinged folding 205 in 201 bottom of battery case, 205 bottom of folding are equipped with one group of fixing clamp 206, and fixing clamp 206 is equipped with Orange glasses 3.Orange glasses 3 include orange eyeglass 301, are equipped with U-shaped wing of nose groove 302 in the middle part of orange eyeglass 301.Orange glasses Between 3 and battery case 201 using the above structure, installation, disassembly and the use of orange glasses 3 are facilitated.
Embodiment 2: smaller green leaf hopper ovum observation method
1) in the tender tea branch of tea place smaller green leaf hopper Onset acquisition 10cm long children;
2) it takes back tea stem cutting behind laboratory in long 10cm, the water suction flower mud of wide 5cm, high 4cm;
3) flower mud for being inserted with tea branch is taken in darkroom;
4) a kind of smaller green leaf hopper ovum observation device of embodiment 1 is worn to head, adjusts fixing head band 1, opens blue-ray light 2, light Beam impinges upon on tea branch, and the smaller green leaf hopper ovum of aobvious fluorescent green is observed by orange glasses 3, and observing effect is as illustrated in Figures 5 and 6.

Claims (9)

1. a kind of smaller green leaf hopper ovum observes device, it is characterised in that including fixing head band (1), the indigo plant being connect with fixing head band (1) Light lamp (2) and the orange glasses (3) connecting with blue-ray light (2), the blue-ray light (2) includes battery case (201), described The positive Middle face of battery case (201) is equipped with lamp cap (202), is equipped with blue light source (208) in the lamp cap (202), described The hinged folding in battery case (201) bottom (205), the folding (205) bottom are equipped with one group of fixing clamp (206), and described consolidates Clamp (206) is equipped with orange glasses (3).
2. a kind of smaller green leaf hopper ovum as described in claim 1 observes device, it is characterised in that the fixing head band (1) includes The endless belt (101) that both ends and blue-ray light (2) are fixed, it is described in the middle part of the endless belt (101) fixed horizontal stripes (102) It is fixed at the top of horizontal stripes (102) other end and blue-ray light (2).
3. a kind of smaller green leaf hopper ovum as described in claim 1 observes device, it is characterised in that the blue light source (208) is adopted Use XP-E LED bulb as light source, laser emission wavelength is 445nm blue light.
4. a kind of smaller green leaf hopper ovum as described in claim 1 observes device, it is characterised in that described battery case (201) side Equipped with blue light signal controlled switch (203).
5. a kind of smaller green leaf hopper ovum as described in claim 1 observes device, it is characterised in that the orange glasses (3) include Orange eyeglass (301), orange eyeglass (301) middle part are equipped with U-shaped wing of nose groove (302).
6. a kind of smaller green leaf hopper ovum as claimed in claim 2 observes device, it is characterised in that described blue-ray light (2) both ends are set There is the first buckle (204) for connecting with endless belt (101), is equipped at the top of the blue-ray light (2) and is used for and horizontal stripes (102) the second buckle (207) of connection.
7. a kind of smaller green leaf hopper ovum as claimed in claim 2 observes device, it is characterised in that set on the endless belt (101) There is the adjusting buckle (103) for adjustment ring shape band (101) length.
8. a kind of observation method of smaller green leaf hopper ovum, it is characterised in that under dark condition, irradiate smaller green leaf hopper by blue light Ovum recycles orange eyeglass observation smaller green leaf hopper ovum.
9. observation method as claimed in claim 8, it is characterised in that specifically includes the following steps:
1) in the tender tea branch of tea place smaller green leaf hopper Onset acquisition 10cm long children;
2) it takes back tea stem cutting behind laboratory in long 10cm, the water suction flower mud of wide 5cm, high 4cm;
3) flower mud for being inserted with tea branch is taken in darkroom;
4) a kind of smaller green leaf hopper ovum observation device described in claim 1 is worn to head, is opened blue-ray light (2), light beam impinges upon On tea branch, the smaller green leaf hopper ovum of aobvious fluorescent green is observed by orange glasses (3).
CN201910040693.XA 2019-01-16 2019-01-16 A kind of smaller green leaf hopper ovum observation device and smaller green leaf hopper ovum observation method Pending CN109633932A (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2293843Y (en) * 1997-03-28 1998-10-07 杨智恒 Electronic night-view glasses
CN201691126U (en) * 2010-02-09 2011-01-05 仇星火 Mining protection helmet
CN206688171U (en) * 2017-03-30 2017-12-01 廖以勒 A kind of intelligent training VR rackets
CN107678180A (en) * 2017-11-10 2018-02-09 闻海强 For neuropathy section can wear-type can secured adjusted formula operating loupe
CN107764783A (en) * 2016-08-23 2018-03-06 黄伙水 Leafhopper ovum fast-positioning device and application method in a kind of host plant body
CN208000412U (en) * 2018-04-17 2018-10-23 宁波大学 For zoopery magnifying glasses for operation
CN209343067U (en) * 2019-01-16 2019-09-03 中国农业科学院茶叶研究所 A kind of smaller green leaf hopper ovum observation device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2293843Y (en) * 1997-03-28 1998-10-07 杨智恒 Electronic night-view glasses
CN201691126U (en) * 2010-02-09 2011-01-05 仇星火 Mining protection helmet
CN107764783A (en) * 2016-08-23 2018-03-06 黄伙水 Leafhopper ovum fast-positioning device and application method in a kind of host plant body
CN206688171U (en) * 2017-03-30 2017-12-01 廖以勒 A kind of intelligent training VR rackets
CN107678180A (en) * 2017-11-10 2018-02-09 闻海强 For neuropathy section can wear-type can secured adjusted formula operating loupe
CN208000412U (en) * 2018-04-17 2018-10-23 宁波大学 For zoopery magnifying glasses for operation
CN209343067U (en) * 2019-01-16 2019-09-03 中国农业科学院茶叶研究所 A kind of smaller green leaf hopper ovum observation device

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