CN109626595B - Water quality purifying microorganism ecological unit, water quality purifying microorganism ecological system and method thereof - Google Patents
Water quality purifying microorganism ecological unit, water quality purifying microorganism ecological system and method thereof Download PDFInfo
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/327—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The embodiment of the invention discloses a water quality purifying microorganism ecological unit, which comprises a frame, wherein a water inlet and a water outlet are respectively arranged on the outer wall surface of the bottom end of the frame, and a filter material layer and a water outlet pipeline are arranged in the frame; the filter material layer is divided into a first accommodating cavity and a second accommodating cavity from top to bottom, the water outlet pipeline is provided with a water inlet end and a water outlet end, the water inlet end is positioned in the first accommodating cavity, the water inlet end is smaller than the height of the top of the frame relative to the horizontal plane, the water outlet end is communicated with the water outlet, the second accommodating cavity is communicated with the water inlet, the filter material layer is formed by stacking a plurality of volcanic ash soil particles, and VBNC strains containing nitrogen and phosphorus removal are implanted in the volcanic ash soil particles. By implanting heterotrophic denitrification phosphorus accumulating bacteria into volcanic ash soil particles, the activation of microorganisms with local functions is improved, stable dominant bacteria are formed, and the aim of improving the water quality purifying effect is fulfilled.
Description
Technical Field
The embodiment of the invention relates to the technical field of water treatment, in particular to a water quality purifying microbial ecological unit, a water quality purifying microbial ecological system and a method thereof.
Background
Water is a source of life, and about seventy percent of human body is composed of water, so that the polluted water body is purified, and the natural ecological environment is restored to great significance in the late generation. The water eutrophication is mainly caused by exogenous and endogenous pollution, the former comprises domestic sewage rich in nitrogen and phosphorus from industrial and agricultural, urban and rural areas and road surface runoff enters water areas including rural entrance ponds, rivers and lakes, the latter mainly comprises floating algae burst after eutrophication, and rich animal and plant remains are deposited on sludge to finally form eutrophication water. At present, the main measures for eutrophic water body treatment are as follows: firstly, physical treatment methods, including diversion of rain and sewage of a sewage draining nano tube for environmental remediation, dredging of bottom mud, dilution of an external clean water source, enhanced oxygenation aeration and the like, can achieve a temporary or slow natural purification effect, but practice shows that the water quality purification problem of the eutrophic water body cannot be effectively solved by using the physical method alone. Secondly, the biological treatment method using animals and plants comprises the following main methods: firstly, a water pump is arranged to enable water to flow and circulate so as to enhance dissolved oxygen in water and improve the water quality purification efficiency of aerobic bacteria, but the water pump is used to improve the aeration and oxygenation of water circulation, consume energy and simultaneously do not filter through a microorganism carrier, and a large number of microorganisms in a non-functional VBNC state are difficult to achieve the comprehensive effect; secondly, nitrifying and denitrifying bacteria are put in, the microorganism composition in water is regulated to enhance the denitrification and dephosphorization water quality purification effect, but external strains are directly put in a water area, and the stable and sustainable effect cannot be achieved because no carrier capable of stabilizing inhabitation and no compatibility with local indigenous microorganism groups are formed, so that dominant bacteria are difficult to form and gradually degenerate and die; thirdly, planting landscape plants, including directly planting aquatic plants and planting in-situ plants based on artificial floating islands to absorb nitrogen and phosphorus nutrition and improve dissolved oxygen of water bodies to achieve a certain water quality purifying effect, but a large number of in-situ artificial floating island plants are easy to cause secondary pollution, and the cost of seasonal planting and plant remains removal is high; fourthly, when blue algae are grown, artificially cultured algae eating insects are put in, although the blue algae have obvious water-cleaning sensory effect, the water quality cannot be regulated, and researches show that as the organic matter remains rich in protein and remains in blue algae eruption and accumulates in the sediment of a water area, a great amount of virulent H 2 S is generated by anaerobic fermentation, once the overwintering eggs of the algae eating insects which are usually sunk in the sediment are contacted with the toxic matter H 2 S of the sediment of the water area, the eggs die directly or after hatching, the larvae die, the population gradually dies, and finally ecological food chain fracture is caused. In the existing biological treatment method of the eutrophic water body, although the sensory effect that algae eating insects are added to reduce the total amount of blue algae cells and improve the transparency of the water body is realized, the algae eating insects added to ① still face the abuse that the algae eating insects cannot safely overwintere, the population base is difficult to maintain, and the symptoms and the root causes are not treated; ② Although the total amount of blue algae cells is reduced, the water quality cannot be effectively regulated, and a sustainable ecological food chain relation cannot be formed. The existing method is a scattered purification mode, the effect on the flowing water body water area is not ideal, the effect on the non-flowing water body water area is very slow, and the effect can be maintained by continuously adding external microorganisms for more than 2 years, so that the purpose of sustainable water quality purification cannot be realized.
Disclosure of Invention
The embodiment of the invention aims to provide a water quality purifying microbial ecological unit, a water quality purifying microbial ecological system and a method thereof, which solve the problem of sustainable water quality purification of eutrophic water.
In order to solve the technical problems, the embodiment of the invention provides a water quality purifying microorganism ecological unit, which comprises a frame, wherein a water inlet and a water outlet are respectively arranged on the outer wall surface of the bottom end of the frame, and a filter material layer and a water outlet pipeline are arranged in the frame; the filter material layer is divided into a first accommodating cavity and a second accommodating cavity from top to bottom, the water outlet pipeline is provided with a water inlet end and a water outlet end, the water inlet end is positioned in the first accommodating cavity, the water inlet end is smaller than the height of the top of the frame relative to the horizontal plane, the water outlet end is communicated with the water outlet, the second accommodating cavity is communicated with the water inlet, the filter material layer is formed by stacking a plurality of volcanic ash soil particles, and VBNC strains containing nitrogen and phosphorus removal are implanted in the volcanic ash soil particles.
The embodiment of the invention also provides a water quality purifying microbial ecological system, which comprises a plurality of water quality purifying microbial ecological units, wherein the water quality purifying microbial ecological units share the same water circulation flow water pump, the water outlets of the adjacent 2 water quality purifying microbial ecological units are mutually communicated, and the water circulation flow water pump realizes the water circulation of the whole water quality purifying microbial ecological system by extracting water in the water outlet of one water quality purifying microbial ecological unit.
The embodiment of the invention also provides a method for purifying microorganism ecology by adopting the water quality, wherein the water to be purified flows into the second accommodating cavity from the water inlet, then enters the first accommodating cavity after being filtered by the filter material layer, flows to the water outlet from the water inlet end of the water outlet pipeline, and finally is pumped by the water circulating flow water pump.
Compared with the prior art, the embodiment of the invention has the following advantages:
1. the volcanic ash soil particles are implanted with VBNC heterotrophic denitrification phosphorus accumulating flora capable of being cultivated, so that the activation of microorganisms for regulating and controlling local functions is improved, stable dominant flora is formed, and the aim of improving the water quality purifying effect is fulfilled;
2. the volcanic ash soil particles with high specific surface area (the permeability is superior to that of activated carbon, the specific surface area of the volcanic ash soil particles with 2 ten thousand M 2/L is equivalent to 40 tennis courts per liter of carrier) are used, so that the volcanic ash soil particles are suitable for the perching of high-efficiency degradable microorganisms, the specific surface area of an effective biological film is greatly improved, the water quality purification efficiency is improved, the contact area between the eutrophic water body and the volcanic ash soil particles is increased, and a low-energy-consumption and high-efficiency circulating water quality purification system is realized;
3. A semi-closed suspended composite artificial floating island device is formed in the first accommodating cavity, so that algae eating insects are far away from toxic substance generation source sediment, the device is completely different from a common open artificial floating island, plants, lower layers and bottoms which absorb nitrogen and phosphorus nutrition substances in a captive mode and have good landscape sensory effects are suitable for algae eating insects to reproduce, and eggs formed by the algae eating insects in winter are prevented from sinking into the toxic sediment to safely overwinter in the water quality purifying microbial ecological unit. Meanwhile, the semi-closed suspended artificial floating island device can prevent filter feeding fishes from entering, keep population quantity with a certain base number which can be dynamically opposite to blue algae, gradually realize sustainable natural ecological food chain relation, and therefore effectively recover natural ecological purification function fundamentally;
4. the volcanic ash soil particles with high specific surface area, VBNC strains with nitrogen and phosphorus removal and the algae feeding insect safe overwintering artificial floating island device are combined to form an integrated multiple ecological water quality purifying system, so that the sustainable eutrophication water quality purifying target is achieved.
In addition, the VBNC strain containing nitrogen and phosphorus removal comprises one or a combination of a plurality of species of the genus Arthrobacter, rhodococcus, amycolatopsis, micrococcus, pallor, pseudomonas stutzeri, alcaligenes, moraxella, acinetobacter and Bacillus cereus, which are the culturable VBNC strains which can be found and obtained by the current non-general traditional microorganism separation method, and the cell number of the activated VBNC strain is enhanced from 5.3X10 7 (number per gram of carrier) to 2.1X10 10 (number per gram of carrier) through the recovery promoting effect of the active signal protein (recovery promoting factor Rpf) secreted by one species of micrococcus, so that the total amount of activated cells of 2-3 orders of magnitude is enhanced, thereby realizing higher nitrogen and phosphorus removal effect.
The diameter of the pozzolanic soil particles is 1 to 5 ten thousand M 2 per liter of the pozzolanic soil particles per 2 to 6 mm.
In addition, the filter material layer comprises a first layer and a second layer which are stacked from top to bottom, wherein the particle size of the volcanic ash soil particles in the first layer is smaller than that of the volcanic ash soil particles in the second layer.
The particle size of the pozzolanic soil particles in the first layer is 2 to 4mm, and the particle size of the pozzolanic soil particles in the second layer is 4 to 6mm.
In addition, the cross section of the frame is square, the outer wall of the bottom end part of the frame is in a fence shape, and a water inlet is formed by gaps between 2 adjacent fences.
In addition, the frame is formed by piling up the fragment of brick, and the bottom of frame is fixed in on the base, and the base is built by cement.
In addition, the water circulation flow pump is arranged on the water outlet, the frame is provided with a bracket for planting plants, and the edge of the bracket is fixed on the inner wall of the frame.
Drawings
One or more embodiments are illustrated by way of example and not limitation in the figures of the accompanying drawings, in which like references indicate similar elements, and in which the figures of the drawings are not to be taken in a limiting sense, unless otherwise indicated.
FIG. 1 is a schematic view showing the structure of a water quality purifying microorganism ecological unit in example 1 of the present invention;
FIG. 2 is a sectional view showing the structure of an ecological unit for purifying water in example 1 of the present invention;
FIG. 3 is a scanning electron microscope image of pozzolanic soil particles in example 1 of the present invention;
FIG. 4 is a schematic view showing the structure of a water purification microorganism ecological system in example 2 of the present invention.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the embodiments of the present application will be described in detail below with reference to the accompanying drawings. However, it will be understood by those of ordinary skill in the art that in various embodiments of the present application, numerous specific details are set forth in order to provide a thorough understanding of the present application. The claimed application may be practiced without these specific details and with various changes and modifications based on the following embodiments.
Example 1
The water quality purifying microbial ecological unit comprises a frame 1, wherein a water inlet 1-1 and a water outlet 1-2 are respectively arranged on the outer wall surface of the bottom end of the frame 1, the cross section of the frame 1 is square, the outer wall of the bottom end part of the frame 1 is in a fence shape, and a water inlet 1-1 is formed by gaps between two adjacent fences. The frame 1 is internally provided with a filter material layer 2 and a water outlet pipeline 3; the filter material layer 2 is divided into a first accommodating cavity 1-3 and a second accommodating cavity 1-4 from top to bottom, the water outlet pipeline 3 is provided with a water inlet end 3-1 and a water outlet end 3-2, the water inlet end 3-1 is positioned in the first accommodating cavity 1-3, the height of the water inlet end 3-1 relative to the horizontal plane is smaller than that of the top end of the frame 1 relative to the horizontal plane, the water outlet end 3-2 is communicated with the water outlet 1-2, the second accommodating cavity 1-4 is communicated with the water inlet 1-1, the filter material layer 2 is formed by stacking a plurality of volcanic soil particles 4, and VBNC strains containing nitrogen and phosphorus removal are implanted in the volcanic soil particles 4.
It should be noted that, by using the conventional culture method, only 0.01-10% of the culturable microorganisms can be separated from the eutrophic water (the eutrophic water refers to the phenomenon that nutrient substances such as nitrogen, phosphorus and the like required by organisms enter slow-flowing water such as lakes, estuaries and gulf in a large amount under the influence of human activities, and cause algae and other plankton to rapidly reproduce, the dissolved oxygen of the water is reduced, the water quality is deteriorated, and fishes and other organisms die in a large amount), most of the microorganisms are in a living but non-culturable (VBNC) state, and in this embodiment, the resuscitation promoting factor Rpf is used for finding and separating the VBNC strain for denitrification and dephosphorization from the eutrophic water, specifically, the VBNC strain can recover activity to perform metabolic reproduction under the stimulation induction of the resuscitation promoting factor Rpf.
Preferably, the VBNC species having nitrogen and phosphorus removal is selected from the group consisting of one or more species of the genus Arthrobacter, rhodococcus, amycolatopsis, micrococcus, xanthium, pseudomonas stutzeri, alcaligenes, moraxella, acinetobacter, bacillus cereus species, in particular, the species being culturable VBNC species which are found and obtained by the presently non-general conventional microorganism separation methods, the recovery promoting effect of the active signal protein (recovery promoting factor Rpf) secreted by one species of the genus Micrococcus is such that the cell number of the activated VBNC species is enhanced from 5.3X10 7 (per g carrier) to 2.1X10 10 (per g carrier), the total amount of activated cells of 2-3 orders of magnitude is enhanced, thereby achieving a higher nitrogen and phosphorus removal effect.
It is worth mentioning that the main component of the pozzolanic soil particles consists of pozzolanic soil with strong physical adsorption property and insoluble water, a proper amount of bentonite, zeolite and the like, and the granular filter carrier material formed by low-temperature sintering has good physical adsorption and chemical neutralization properties (the pH value of the pozzolanic soil particles can be stabilized between 5.5 and 7.0 when the pozzolanic soil particles are used for treating general eutrophic water for 10 months). As shown in FIG. 3, the diameter of the pozzolanic soil particles 4 is 1 to 5 ten thousand M 2 per liter of the specific surface area of the pozzolanic soil particles 4 per 2 to 6 mm. Specifically, the specific surface area of the pozzolanic soil particles 4 in the embodiment is 2 ten thousand M 2/L, which is equivalent to 40 tennis courts per liter of carrier, so that the method is suitable for high-efficiency degradable microorganism inhabitation, greatly improves the specific surface area of a filter material layer, improves the water quality purification efficiency, and simultaneously utilizes a multiple aerobic (partial anaerobic) engineering process to improve the contact flux of the eutrophic water body and the filter material layer, thereby realizing a water quality purification system with low energy consumption and high efficiency circulation.
The filter layer 2 includes a first layer 2-1 and a second layer 2-2 stacked from top to bottom, and the particle size of the pozzolanic soil particles 4 in the first layer 2-1 is smaller than the particle size of the pozzolanic soil particles 4 in the second layer 2-2. Specifically, the particle size of the pozzolanic soil particles 4 in the first layer 2-1 is 2 to 4mm, and the particle size of the pozzolanic soil particles 4 in the second layer 2-2 is 4 to 6mm.
Specifically, the frame 1 may be formed by stacking bricks, and the bottom of the frame 1 is fixed on the base 5, and the base 5 may be formed by cement, however, other materials, such as wood, plastic, stainless steel, or a combination thereof, may be used for the frame 1, which is not limited herein. The frame 1 is fixed on the water bottom of the eutrophic water area through the base 5, so that the strength of the frame 1 is enhanced.
Furthermore, a bracket 7 for planting plants 6 is provided on the frame 1, and the edge of the bracket 7 is fixed to the inner wall of the frame 1.
It is noted that the water quality purifying microorganism ecological unit is also provided with a sludge discharge standby pipe 8 and a sludge discharge standby pipe pump 9 connected with the sludge discharge standby pipe 8, and the sludge discharge standby pipe 8 passes through the base 5. Specifically, when a certain amount of sludge is formed under the condition of sudden overload exogenous pollution interference, the sludge discharge standby pipe pump 9 needs to be started for sewage discharge, so that the running time of the water quality purification microorganism ecological unit is prolonged.
In addition, the water quality purifying microbial ecological unit in this embodiment further sets a microbial reinforced adding standby pipe 10 inside the frame 1, specifically, an opening of the microbial reinforced adding standby pipe 10 is located at the top end of the frame 1, and another opening of the microbial reinforced adding standby pipe 10 is located on the filter material layer 2, when an exogenous pollution strong load occurs, VBNC strain with nitrogen and phosphorus removal can be added through the microbial reinforced adding standby pipe 10, so as to prolong the time of water quality purifying.
In addition, the water outlet pipeline 3 in this embodiment is in an inverted T shape, wherein the water inlet end is located at the end of the T-shaped "I", the outlet ends are located at two sides of the T-shaped "a", in addition, the water quality purifying microbial ecological unit in this embodiment further includes a water circulation flow water pump 11 connected to the outlet end, a low-lift circulation water outlet 12 connected to the water circulation flow water pump 11, and a landscape water spraying standby water outlet 13 connected to the water circulation flow water pump 11, specifically, the water circulation flow water pump 11 pumps out water flowing out of the water outlet pipeline 3, a part of water is filtered and purified by the low-lift circulation water outlet 12 in a secondary circulation, and another part of water forms landscape water spraying through the landscape water spraying standby water outlet 13.
Specifically, when the water quality purifying microorganism ecological unit is used for treating the eutrophic water area, the top end of the frame 1 is higher than the water level, the height of the water inlet end 3-1 is lower than the water level, the water pressure inside and outside the frame 1 is utilized to naturally enter the eutrophic water area from the water inlet 1-1 into the frame body 1, the eutrophic water area with the algae eating insects is absorbed and degraded by volcanic ash soil particles 4 and VBNC bacteria attached to the water area and having denitrification and dephosphorization, and the water after disposable purification flows to the water outlet 1-2 through the water inlet end 3-1 and is discharged out of the frame 1. It should be noted that the water inlet end 3-1 is lower than the water level of the water surface, so that purified water can be ensured to flow out through the water inlet end 3-1.
It should be noted that, because the water in the frame 1 flows and always flows out of the water outlet 1-2 through the water inlet end 3-1, a semi-closed suspended composite artificial floating island is formed between the water surface in the frame 1 and the top end of the frame 1, the upper layer of the inner part of the artificial floating island is used for housing plants absorbing nitrogen and phosphorus nutrients, the lower layer and the bottom of the artificial floating island are not only suitable for the propagation of algae eating insects, but also eggs formed in winter are not sunk into toxic bottom mud so as to safely overwinter in the artificial floating island. Meanwhile, the semi-closed suspended artificial floating island device can prevent filter fishes from entering, keep population quantity which can dynamically face to a certain base number with blue algae, and gradually realize sustainable natural ecological food chaining relation. Therefore, the natural ecological purification function can be effectively restored fundamentally.
Example 2
A water quality purifying microbial ecological system, as shown in FIG. 4, comprises a plurality of water quality purifying microbial ecological units as described in embodiment 1, wherein the water quality purifying microbial ecological units share the same water circulation flow water pump, the water outlets of the adjacent 2 water quality purifying microbial ecological units are communicated with each other, and the water circulation flow water pump realizes the water circulation of the whole water quality purifying microbial ecological system by extracting water in the water outlet of one water quality purifying microbial ecological unit.
The water circulation flow water pump controls the water circulation of the whole water quality purifying microbial ecological system, improves the water circulation speed of the water quality purifying microbial ecological system, avoids using a plurality of water circulation flow water pumps and saves the cost.
Specifically, the water quality purifying microorganism ecological system in the embodiment is formed by connecting 2 water quality purifying microorganism ecological units in series, and water outlets of the 2 water quality purifying microorganism ecological units are communicated through a tower type serial engineering base joint. It should be noted that, the water quality purifying microbial ecological system in this embodiment may also be formed by connecting 3,4,5 water quality purifying microbial ecological units in series or in parallel, which is not described in detail herein.
Example 3
Indoor purification experiment of eutrophic water body
The method comprises the steps of carrying out continuous flow pilot-scale experiments on eutrophic water obtained by artificial formula design and inoculated with natural blue-green algae in a bioreactor, wherein the total phosphorus concentration, the total nitrogen concentration and the chlorophyll a concentration of a water sample are respectively (22.1+/-1.9) mg/L, (16.1+/-2.1) mg/L, (220.1+/-23.5) mug/L, and the average removal rate of Total Phosphorus (TP), total Nitrogen (TN) and chlorophyll a (Chla) respectively reaches 94.2%,91.0% and 93.9% after 90 days of operation, and the three indexes can be maintained to be stable over 90% after continuous debugging operation for 24 months.
Example 4
Printing and dyeing wastewater indoor continuous flow operation experiment based on bioreactor
After being implanted with VBNC strain volcanic soil particles with denitrification and dephosphorization, the result shows that the recovery activated Rpf signal protein (1000 pmol) generated by the Rpf generating strain feeding device can at least reduce 150mg/L of CODcr of printing and dyeing wastewater discharged after large-scale bioengineering treatment by 20-30%, and the activity and degradation effect of functional microorganisms in a bioreactor are improved.
Through indoor experiments, the total bacterial detection amount is found in the reactor by using a recovery promoting factor Rpf: the control group is 5.3X107-2.4X109, and the experimental group is 2.9X109-2.1X106, and the bacterial detection abundance is improved by 8.7-54.7 times. The first time that a large number of VBNC (viable but non-culturable) microorganisms which are not isolated by conventional isolation methods and are susceptible to Rpf in a state similar to dormant state exist in the reactor, wherein the microorganisms have phosphorus accumulating function with the closely-related strains such as Burkholderia sp., bacillus cereus sp., bacillus lysine sp., paenibacillus paenibacillus sp., brevibacterium Brevibacillus sp., bacillus pallidum sp., bacillus sphingans sp., enterobacter sp., etc.; and species closely related to Bacillus cereus sp., enterobacter sp., pseudomonas sp., etc., belong to the denitrification functional flora (16S rDNA sequence similarity 97.6-99.9%). The reactor was run continuously for three months, with average total phosphorus and total nitrogen removal rates of 95.9% and 92.4% respectively (total phosphorus and total nitrogen initial concentrations of 21.6 (+ -1.9) mg/L and 16.8 (+ -1.2) mg/L, respectively).
Example 5
In 100L of artificial eutrophic water (the total amount of initial CODcr is 90mg/L, the total amount of chlorophyll is 0.48 mg/L), submerged planting of plants such as water paste, iron crown and the like, 100 heads (1 head/L) of the stocking branch horn algae eating worm reach about 10 ten thousands heads (1000 heads/L) after 5 days, and the removal rate is 79.6 percent by measuring 18.4mg/L of the total amount of CODcr; the total chlorophyll amount is 0.09mg/L, and the removal rate reaches 81.3 percent. The algae-eating insect eggs in the artificial floating island can safely overwintere outdoors, and the algae-eating insect adults after overwintering are seen in the next spring and summer when the water temperature is above 25 ℃.
Example 6
Use of water quality purifying microbial ecosystem in pond
The water quality of the pond is gradually changed into inferior V-type water quality from the latest external water injection three-level water quality. After three months of continuous operation, the water quality gradually returns to III-IV class. According to the actual condition (with the help of indoor experimental data) that the blue algae are unfavorable at the low temperature of 3-4 months of 12-3 months in winter, no blue algae appear after the running is stopped at intervals, but the water quality index is gradually returned to IV-V class. Continuous operation is carried out along with the rise of the air temperature for 4 months, and the water body reaches the water quality index below grade IV after the water body is operated for 3 months by applying the water quality purifying microbial ecological system in the water pond at the gate, so as to realize the design target.
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples of carrying out the invention and that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (7)
1. The microbial ecological unit for purifying water quality is characterized by comprising a frame, wherein a water inlet and a water outlet are respectively formed in the outer wall surface of the bottom end of the frame, and a filter material layer and a water outlet pipeline are arranged in the frame; the filter material layer is divided into a first accommodating cavity and a second accommodating cavity from top to bottom, the water outlet pipeline is in an inverted T shape, the water outlet pipeline is provided with a water inlet end and two water outlet ends, the water inlet end is positioned at the tail end of an I shape, the water inlet end is positioned in the first accommodating cavity, the height of the water inlet end relative to the horizontal plane is smaller than the height of the top end of the frame relative to the horizontal plane, the top end of the frame is higher than the water level, the height of the water inlet end is lower than the water level and higher than the filter material layer, the two water outlet ends are respectively positioned at the two sides of the first shape, the water outlet ends are communicated with the water outlet, the second accommodating cavity is communicated with the water inlet, the filter material layer is formed by stacking a plurality of volcanic soil particles, the particle size of the volcanic soil particles in the first layer is 2-4 mm, and the volcanic soil particles in the second layer are stacked from top to bottom, and the particle size of the volcanic soil particles in the first layer is 4-4 mm; VBNC strains containing nitrogen and phosphorus removal are planted in the volcanic ash soil particles, and are found and separated from eutrophic water bodies by using a resuscitating promoting factor Rpf;
The water circulation flow water pump is arranged on the water outlet, the low-lift circulation water outlet is connected with the water circulation flow water pump, and the landscape water spraying standby water outlet is connected with the water circulation flow water pump, a bracket for planting plants is arranged on the frame, and the edge of the bracket is fixed on the inner wall of the frame;
a semi-closed suspended composite artificial floating island is formed between the inner water surface of the frame and the top end of the frame, and plants absorbing nitrogen and phosphorus nutrients are planted in the upper layer of the inner part of the artificial floating island, and the lower layer and the bottom of the inner part of the artificial floating island are used for breeding algae eating insects.
2. The water quality purifying microbial ecological unit according to claim 1, wherein the VBNC strain for denitrification and dephosphorization comprises one or more combinations of arthrobacter species, rhodococcus species, amycolatopsis species, micrococcus species, pallidobacter species, pseudomonas stutzeri species, alcaligenes species, moraxella species, acinetobacter species, bacillus cereus species.
3. The water quality purifying microbial ecological unit according to claim 1, wherein the specific surface area of the pozzolanic soil particles per liter is 1 to 5 ten thousand m 2.
4. The water quality purifying microbial ecological unit according to claim 1, wherein the cross section of the frame is square, the outer wall of the bottom end portion of the frame is in a fence shape, and a gap between 2 adjacent fences forms the water inlet.
5. The water quality purifying microbial ecological unit according to claim 4, wherein the frame is formed by stacking bricks, and the bottom of the frame is fixed on a base, and the base is formed by cement.
6. A water quality purifying microbial ecological system, characterized by comprising a plurality of water quality purifying microbial ecological units according to any one of claims 1 to 5, wherein the water quality purifying microbial ecological units share the same water circulation flow water pump, water outlets of the adjacent 2 water quality purifying microbial ecological units are mutually communicated, and the water circulation flow water pump realizes water circulation of the whole water quality purifying microbial ecological system by extracting water in the water outlet of one water quality purifying microbial ecological unit.
7. A method for purifying microbial ecology by using the water quality as described in claim 6, wherein the water to be purified flows into the second accommodating cavity from the water inlet, then flows into the first accommodating cavity after being filtered by the filter material layer, flows to the water outlet from the water inlet end of the water outlet pipeline, and finally is pumped out by the water circulating flow pump.
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