CN109618918A - Open up the breeding method of shape cherry iris - Google Patents
Open up the breeding method of shape cherry iris Download PDFInfo
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- CN109618918A CN109618918A CN201910113697.6A CN201910113697A CN109618918A CN 109618918 A CN109618918 A CN 109618918A CN 201910113697 A CN201910113697 A CN 201910113697A CN 109618918 A CN109618918 A CN 109618918A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of breeding method for opening up shape cherry iris, the breeding method include: be 1) maternal, Lip river with good and cherry iris (Phal.Jiaho Cherry) than iris (Phal.Lobbii) be that male parent progress hybridization pollination obtains hybrid seed；2) hybrid seed is cultivated and selects optimal plant；3) optimal plant is subjected to tissue cultures to obtain exhibition shape cherry iris.There is the exhibition shape cherry iris the small red lip of dusting, the characteristic that flower pattern is excellent, bifurcated is good, while the breeding method to have the advantages that process is simply and easy-operating.
Description
Technical field
The present invention relates to the breeding methods of iris, and in particular, to a kind of breeding method for opening up shape cherry iris.
Background technique
Iris (Phalaenopsis aphrodite Rchb. F.) is orchid family Phalaenopsis, originates in subtropical zone rain
Forest land area, for growing nonparasitically upon another plant property orchid.The coarse aerial root of iris white is exposed at around blade, absorbs nutrient in air in addition to having
Effect is outer, and there are also growth and photosynthesis.In the time in the new year, Phalaenopsis plants extract long bennet out from axil, and output
Flower as dancing in the air shaped like butterfly, the deep favor by flower fans, is known as the title of " cattleya queen consort ".It is distributed in Thailand, Philippine, horse
Come West Asia, Indonesia and TaiWan, China.
Although existing iris has numerous kinds, still do not occur the small red lip of dusting, flower pattern is excellent, bifurcated
The good kind of property.
Summary of the invention
The object of the present invention is to provide a kind of breeding method for opening up shape cherry iris, which has small
Piece red lip of dusting, the characteristic that flower pattern is excellent, bifurcated is good, while the breeding method have the advantages that process is simply and easy-operating.
To achieve the goals above, the present invention provides a kind of breeding methods for opening up shape cherry iris, comprising:
1) with it is good and cherry iris (Phal.Jiaho Cherry) be female parent, Lip river than iris (Phal.Lobbii) for male parent
It carries out hybridization pollination and obtains hybrid seed；
2) hybrid seed is cultivated and selects optimal plant；
3) optimal plant is subjected to tissue cultures to obtain exhibition shape cherry iris.
In the above-mentioned technical solutions, the present invention is maternal, Lip river ratio with good and cherry iris (Phal.Jiaho Cherry)
Iris (Phal.Lobbii) is that male parent progress hybridization pollination obtains hybrid seed, and then hybrid seed is cultivated and selected
Optimal plant is selected out, last tissue cultures are to obtain exhibition shape cherry iris.The exhibition shape cherry iris has small dusting red
Lip, the characteristic that flower pattern is excellent, bifurcated is good, while the breeding method have the advantages that process is simply and easy-operating.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Detailed description of the invention
The drawings are intended to provide a further understanding of the invention, and constitutes part of specification, with following tool
Body embodiment is used to explain the present invention together, but is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is the plant figure for the exhibition shape cherry iris that the embodiment of the present application 1 obtains；
Fig. 2 is the plant figure of the auspicious iris of Liu Shi shellfish in the prior art；
Fig. 3 is single photo figure of iris in Fig. 1；
Fig. 4 is single photo figure of iris in Fig. 2；
Fig. 5 is the exhibition shape cherry iris and the auspicious iris comparison result statistical chart of Liu Shi shellfish that embodiment 1 obtains.
Specific embodiment
Detailed description of the preferred embodiments below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The present invention provides a kind of breeding methods for opening up shape cherry iris, comprising:
1) with it is good and cherry iris (Phal.Jiaho Cherry) be female parent, Lip river than iris (Phal.Lobbii) for male parent
It carries out hybridization pollination and obtains hybrid seed；
2) hybrid seed is cultivated and selects optimal plant；
3) optimal plant is subjected to tissue cultures to obtain exhibition shape cherry iris.
In the present invention, the good and cherry iris specific place of production can select in a wide range, it is contemplated that product
The price of kind, it is preferable that good and cherry iris (Phal.Jiaho Cherry) is by the garden breeding of top grade orchid.
In the present invention, Lip river can select in a wide range than the specific place of production of iris, it is contemplated that kind
Price, it is preferable that Lip river originates in Nepal or Vietnam than iris (Phal.Lobbii).
In step 2) of the invention, the condition of cultivation can select in a wide range, but in order to improve cultivation
Rate and effect, it is preferable that in step 2, the condition of cultivation are as follows: temperature is 25-30 DEG C, relative humidity 60-70%, light
It is 8000-12000lux according to intensity.
In step 2) of the invention, the actual conditions of tissue cultures can select in a wide range, but in order to mention
The rate and effect that height is cultivated, it is preferable that in step 3), tissue cultures are as follows:
1) it takes the bud point of optimal plant and sterilizes, be then carried out at progress Initial culture in initial culture base and obtain callus；
2) it takes the budlet base portion on callus and is cut into segment, be subsequently placed in progress squamous subculture in subculture medium and obtain nothing
Radical bud seedling；
3) unrooted sprout is carried out to culture of rootage in root media until root long 3-4cm progress hardening, is then tamed
Transplanting；
Wherein, initial culture base be MS culture medium+0.1-0.3mg/L folic acid+2-3mg/L 6-BA+1.2-1.5mg/L mashed potatoes+
0.2-0.4mg/L ascorbic acid；Subculture medium is MS culture medium+0.1-0.3mg/L ABA+1.5-2.2mg/L 6-BA+
0.2-0.6mg/L penicillin+15-25g/L sucrose+4-8g/L agar；Root media is 1/2MS culture medium+1-3mg/L inositol
+ 10-18g/L active carbon.
In above-mentioned tissue cultures, the condition of Initial culture can select in a wide range, but in order to further mention
The rate that height is cultivated, it is preferable that the condition of Initial culture are as follows: incubation time is 20-25 days, and temperature is 23-27 DEG C, light application time
For 10-12h/ days, intensity of illumination 1500-2000Lux.
In above-mentioned tissue cultures, the condition of squamous subculture can select in a wide range, but in order to further mention
The rate that height is cultivated, it is preferable that the condition of squamous subculture are as follows: incubation time is 28-30 days, and temperature is 23-27 DEG C, light application time
For 10-12h/ days, intensity of illumination 1500-2000Lux.
In above-mentioned tissue cultures, the condition of culture of rootage can select in a wide range, but in order to further mention
The rate that height is cultivated, it is preferable that the condition of culture of rootage are as follows: temperature is 23-27 DEG C, light application time is 10-12h/ days, illumination is strong
Degree is 1500-2000Lux；The hardening time is 15-20 days.
In above-mentioned tissue cultures, the concrete mode of disinfection can select in a wide range, but in order to further mention
The rate that height is cultivated, it is preferable that sterilize as alcohol immersion 20-30 seconds.
The present invention will be described in detail by way of examples below.In the following embodiments, good and cherry iris
(Phal.Jiaho Cherry) is by the garden breeding of top grade orchid；Lip river originates in Nepal or Vietnam than iris (Phal.Lobbii).
Embodiment 1
1) with it is good and cherry iris (Phal.Jiaho Cherry) be female parent, Lip river than iris (Phal.Lobbii) for male parent
It carries out hybridization pollination and obtains hybrid seed；
2) hybrid seed is cultivated into (temperature is 28 DEG C, relative humidity 65%, intensity of illumination 10000lux) and is selected
Optimal plant；
3) the bud point and alcohol for taking optimal plant are impregnated 25 seconds and are carried out disinfection, and are then carried out at initial culture base (initial culture base
For MS culture medium+0.2mg/L folic acid+2.5mg/L 6-BA+1.3mg/L mashed potatoes+0.3mg/L ascorbic acid) in carry out it is primary
Culture (incubation time is 23 days, and temperature is 25 DEG C, light application time is 11h/ days, intensity of illumination 1800Lux) obtains callus group
It knits；
4) it takes the budlet base portion on callus and is cut into segment, being subsequently placed in subculture medium, (subculture medium is MS culture
Base+0.2mg/L ABA+1.9mg/L 6-BA+0.4mg/L penicillin+20g/L sucrose+6g/L agar) in carry out squamous subculture
(incubation time is 29 days, and temperature is 25 DEG C, light application time is 11h/ days, intensity of illumination 1800Lux) obtains unrooted sprout；
5) by the unrooted sprout, in root media, (root media is 1/2MS culture medium+2mg/L inositol+15g/L activity
Charcoal) in carry out the culture of rootage (condition of culture of rootage are as follows: temperature is 25 DEG C, light application time is 11h/ days, intensity of illumination is
1800Lux；The hardening time is 18 days) until root long 3.5cm carries out hardening, then carry out rooting culture.
Embodiment 2
1) with it is good and cherry iris (Phal.Jiaho Cherry) be female parent, Lip river than iris (Phal.Lobbii) for male parent
It carries out hybridization pollination and obtains hybrid seed；
2) hybrid seed is cultivated into (temperature is 25 DEG C, relative humidity 70%, intensity of illumination 12000lux) and is selected
Optimal plant；
3) the bud point and alcohol for taking optimal plant are impregnated 20 seconds and are carried out disinfection, and are then carried out at initial culture base (initial culture base
For MS culture medium+0.1mg/L folic acid+2mg/L 6-BA+1.2mg/L mashed potatoes+0.2mg/L ascorbic acid) in carry out just be commissioned to train
It supports (incubation time is 20 days, and temperature is 23 DEG C, light application time is 10h/ days, intensity of illumination 1500Lux) and obtains callus；
4) it takes the budlet base portion on callus and is cut into segment, being subsequently placed in subculture medium, (subculture medium is MS culture
Base+0.1mg/L ABA+1.5mg/L 6-BA+0.2mg/L penicillin+15g/L sucrose+4g/L agar) in carry out squamous subculture
(incubation time is 28 days, and temperature is 23 DEG C, light application time is 10h/ days, intensity of illumination 1500Lux) obtains unrooted sprout；
5) by the unrooted sprout, in root media, (root media is 1/2MS culture medium+1mg/L inositol+10g/L activity
Charcoal) in carry out the culture of rootage (condition of culture of rootage are as follows: temperature is 23 DEG C, light application time is 10h/ days, intensity of illumination is
1500Lux；The hardening time is 15 days) until root long 3cm carries out hardening, then carry out rooting culture.
Embodiment 3
1) with it is good and cherry iris (Phal.Jiaho Cherry) be female parent, Lip river than iris (Phal.Lobbii) for male parent
It carries out hybridization pollination and obtains hybrid seed；
2) hybrid seed is cultivated into (temperature is 30 DEG C, relative humidity 60%, intensity of illumination 8000lux) and is selected
Optimal plant；
3) the bud point and alcohol for taking optimal plant are impregnated 30 seconds and are carried out disinfection, and are then carried out at initial culture base (initial culture base
For MS culture medium+0.3mg/L folic acid+3mg/L 6-BA+1.5mg/L mashed potatoes+0.4mg/L ascorbic acid) in carry out just be commissioned to train
It supports (incubation time is 25 days, and temperature is 27 DEG C, light application time is 12h/ days, intensity of illumination 2000Lux) and obtains callus；
4) it takes the budlet base portion on callus and is cut into segment, being subsequently placed in subculture medium, (subculture medium is MS culture
Base+0.1-0.3mg/L ABA+2.2mg/L 6-BA+0.6mg/L penicillin+25g/L sucrose+8g/L agar) in carry out after being commissioned to train
It supports (incubation time is 30 days, and temperature is 27 DEG C, light application time is 12h/ days, intensity of illumination 2000Lux) and obtains unrooted sprout；
5) by the unrooted sprout, in root media, (root media is 1/2MS culture medium+3mg/L inositol+18g/L activity
Charcoal) in carry out the culture of rootage (condition of culture of rootage are as follows: temperature is 27 DEG C, light application time is 12h/ days, intensity of illumination is
2000Lux；The hardening time is 20 days) until root long 4cm carries out hardening, then carry out rooting culture.
Detect example 1
1) obtained iris is cultivated to embodiment 1 cultivate until blooming and observing, the high 39cm of plant, inflorescence is long
25-30cm, the long 40cm of peduncle, Hua Kuan 3.2cm, petal mass-tone is pink；And it is compared with the auspicious iris of Liu Shi shellfish
Compared with, it is specifically shown in Fig. 1-5, as seen from the figure, relative to the auspicious iris of Liu Shi shellfish, small red lip of dusting of iris that the application cultivates,
The kind that flower pattern is excellent, bifurcated is good.The kind is compared with existing kind with apparent distinguishability, further relates to its tool
It is variant.
2) observation embodiment 1 cultivates the obtained various characters of iris SPRING WHEAT BEFORE AND AFTER FLOWERING plant, as the result is shown its SPRING WHEAT BEFORE AND AFTER FLOWERING
Various trait expressions are consistent, and occur without variation, illustrate that its is with uniformity.
3) embodiment 1 cultivated obtained iris and blooms in the first time mitogenetic seedling of in August, 2017, in August second in 2018
Secondary to bloom, performance of blooming is bloomed with first time to show unanimously, further relates to it and has excellent stability.
Wherein, the iris that embodiment 2-3 is cultivated and the iris that embodiment 1 obtains are essentially identical.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (9)
1. a kind of breeding method for opening up shape cherry iris characterized by comprising
1) with it is good and cherry iris (Phal.Jiaho Cherry) be female parent, Lip river than iris (Phal.Lobbii) for male parent
It carries out hybridization pollination and obtains hybrid seed；
2) hybrid seed is cultivated and selects optimal plant；
3) optimal plant is subjected to tissue cultures to obtain the exhibition shape cherry iris.
2. breeding method according to claim 1, which is characterized in that described good and cherry iris (Phal.Jiaho
Cherry) by the garden breeding of top grade orchid.
3. breeding method according to claim 1, which is characterized in that the Lip river is originated in than iris (Phal.Lobbii)
In Nepal or Vietnam.
4. breeding method according to claim 1, which is characterized in that in step 2, the condition of the cultivation are as follows: temperature
It is 25-30 DEG C, relative humidity 60-70%, intensity of illumination 8000-12000lux.
5. breeding method according to claim 1, which is characterized in that in step 3), the tissue cultures are as follows:
1) it takes the bud point of optimal plant and sterilizes, be then carried out at progress Initial culture in initial culture base and obtain callus；
2) it takes the budlet base portion on callus and is cut into segment, be subsequently placed in progress squamous subculture in subculture medium and obtain nothing
Radical bud seedling；
3) the unrooted sprout is carried out to culture of rootage in root media until root long 3-4cm progress hardening, then carries out
Rooting culture；
Wherein, initial culture base be MS culture medium+0.1-0.3mg/L folic acid+2-3mg/L 6-BA+1.2-1.5mg/L mashed potatoes+
0.2-0.4mg/L ascorbic acid；The subculture medium is MS culture medium+0.1-0.3mg/L ABA+1.5-2.2mg/L 6-BA
+ 0.2-0.6mg/L penicillin+15-25g/L sucrose+4-8g/L agar；The root media is 1/2MS culture medium+1-3mg/
L inositol+10-18g/L active carbon.
6. breeding method according to claim 5, which is characterized in that the condition of the Initial culture are as follows: incubation time is
20-25 days, temperature was 23-27 DEG C, light application time is 10-12h/ days, intensity of illumination 1500-2000Lux.
7. breeding method according to claim 5, which is characterized in that the condition of the squamous subculture are as follows: incubation time is
28-30 days, temperature was 23-27 DEG C, light application time is 10-12h/ days, intensity of illumination 1500-2000Lux.
8. breeding method according to claim 5, which is characterized in that the condition of the culture of rootage are as follows: temperature 23-27
DEG C, light application time be 10-12h/ days, intensity of illumination 1500-2000Lux；The hardening time is 15-20 days.
9. breeding method according to claim 5, which is characterized in that the disinfection is that alcohol impregnates 20-30 seconds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910113697.6A CN109618918A (en) | 2019-02-14 | 2019-02-14 | Open up the breeding method of shape cherry iris |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910113697.6A CN109618918A (en) | 2019-02-14 | 2019-02-14 | Open up the breeding method of shape cherry iris |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009284812A (en) * | 2008-05-29 | 2009-12-10 | Seisan Ryu | Phalaenopsis named "kyokuto whiskey" |
| CN102893851A (en) * | 2012-06-19 | 2013-01-30 | 漳州钜宝生物科技有限公司 | Butterfly orchid breeding method |
| WO2015101009A1 (en) * | 2013-12-30 | 2015-07-09 | 佛山市顺德区今日景艺生物科技有限公司 | Inducing medium for butterfly orchid and method for improving survival rate of butterfly orchid pedicel |
| CN107691221A (en) * | 2017-11-10 | 2018-02-16 | 佛山市恒爱网络科技有限公司 | A kind of iris tissue culture method |
| CN108684525A (en) * | 2018-05-16 | 2018-10-23 | 芜湖市三山区绿色食品产业协会 | The method for tissue culture of iris |
-
2019
- 2019-02-14 CN CN201910113697.6A patent/CN109618918A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009284812A (en) * | 2008-05-29 | 2009-12-10 | Seisan Ryu | Phalaenopsis named "kyokuto whiskey" |
| CN102893851A (en) * | 2012-06-19 | 2013-01-30 | 漳州钜宝生物科技有限公司 | Butterfly orchid breeding method |
| WO2015101009A1 (en) * | 2013-12-30 | 2015-07-09 | 佛山市顺德区今日景艺生物科技有限公司 | Inducing medium for butterfly orchid and method for improving survival rate of butterfly orchid pedicel |
| CN107691221A (en) * | 2017-11-10 | 2018-02-16 | 佛山市恒爱网络科技有限公司 | A kind of iris tissue culture method |
| CN108684525A (en) * | 2018-05-16 | 2018-10-23 | 芜湖市三山区绿色食品产业协会 | The method for tissue culture of iris |
Non-Patent Citations (4)
| Title |
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| HWA-TUNG LIU: "Orchid Hybrid:P halaenopsis Liu’s Cute Angel", 《OCHIDS.ORG》 * |
| SOLANUM: "Phal. Liu’s Cute Angel", 《LONIS ORCHIDEENFORUM》 * |
| WEE-PENG GOW等: "Influence of growth regulators on direct embryo formation from leaf explants of Phalaenopsis orchids", 《ACTA PHYSIOLOGIAE PLANTARUM》 * |
| 李娜: "蝴蝶兰组培快繁技术的研究进展", 《广东农业科学》 * |
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Application publication date: 20190416 |