CN109607764A - A kind of pre- film fluidisation bed fillers and the preparation method and application thereof - Google Patents
A kind of pre- film fluidisation bed fillers and the preparation method and application thereof Download PDFInfo
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- CN109607764A CN109607764A CN201910138237.9A CN201910138237A CN109607764A CN 109607764 A CN109607764 A CN 109607764A CN 201910138237 A CN201910138237 A CN 201910138237A CN 109607764 A CN109607764 A CN 109607764A
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- Prior art keywords
- filler
- nitrobacteria
- actinomyces
- film
- bacterium solution
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- 239000000945 filler Substances 0.000 title claims abstract description 142
- 238000005243 fluidization Methods 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 241000108664 Nitrobacteria Species 0.000 claims abstract description 47
- 241000186046 Actinomyces Species 0.000 claims abstract description 38
- 230000000694 effects Effects 0.000 claims abstract description 29
- 239000000463 material Substances 0.000 claims abstract description 26
- 244000005700 microbiome Species 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 230000005484 gravity Effects 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 53
- 241000194107 Bacillus megaterium Species 0.000 claims description 36
- 244000063299 Bacillus subtilis Species 0.000 claims description 34
- 230000000243 photosynthetic effect Effects 0.000 claims description 28
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 27
- AHEWZZJEDQVLOP-UHFFFAOYSA-N monobromobimane Chemical compound BrCC1=C(C)C(=O)N2N1C(C)=C(C)C2=O AHEWZZJEDQVLOP-UHFFFAOYSA-N 0.000 claims description 24
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 21
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 13
- 238000011049 filling Methods 0.000 claims description 13
- 229910052698 phosphorus Inorganic materials 0.000 claims description 13
- 239000011574 phosphorus Substances 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 claims description 8
- -1 polyethylene Polymers 0.000 claims description 8
- 229920000903 polyhydroxyalkanoate Polymers 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- 229910021536 Zeolite Inorganic materials 0.000 claims description 7
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- 239000010457 zeolite Substances 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 6
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 claims description 6
- 239000004626 polylactic acid Substances 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims description 5
- 230000010148 water-pollination Effects 0.000 claims description 5
- 241000187844 Actinoplanes Species 0.000 claims description 4
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 4
- 229920001155 polypropylene Polymers 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 claims description 3
- 239000000347 magnesium hydroxide Substances 0.000 claims description 3
- 229910001862 magnesium hydroxide Inorganic materials 0.000 claims description 3
- 241000186361 Actinobacteria <class> Species 0.000 claims description 2
- 229920001817 Agar Polymers 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 2
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 241000190984 Rhodospirillum rubrum Species 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 159000000013 aluminium salts Chemical class 0.000 claims description 2
- 229910000329 aluminium sulfate Inorganic materials 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 229920001577 copolymer Polymers 0.000 claims description 2
- 239000008101 lactose Substances 0.000 claims description 2
- 159000000003 magnesium salts Chemical class 0.000 claims description 2
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 2
- 150000002696 manganese Chemical class 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
- 239000010703 silicon Substances 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims 3
- MQIUGAXCHLFZKX-UHFFFAOYSA-N Di-n-octyl phthalate Natural products CCCCCCCCOC(=O)C1=CC=CC=C1C(=O)OCCCCCCCC MQIUGAXCHLFZKX-UHFFFAOYSA-N 0.000 claims 1
- BJQHLKABXJIVAM-UHFFFAOYSA-N bis(2-ethylhexyl) phthalate Chemical compound CCCCC(CC)COC(=O)C1=CC=CC=C1C(=O)OCC(CC)CCCC BJQHLKABXJIVAM-UHFFFAOYSA-N 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 229920001896 polybutyrate Polymers 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 42
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 21
- 239000002068 microbial inoculum Substances 0.000 abstract description 5
- 238000006396 nitration reaction Methods 0.000 abstract description 5
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 abstract description 3
- 230000003197 catalytic effect Effects 0.000 abstract description 3
- 238000003860 storage Methods 0.000 abstract description 3
- 239000002028 Biomass Substances 0.000 description 22
- 238000000034 method Methods 0.000 description 17
- 230000000813 microbial effect Effects 0.000 description 16
- 230000000052 comparative effect Effects 0.000 description 12
- 230000008569 process Effects 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 239000010865 sewage Substances 0.000 description 9
- 238000009629 microbiological culture Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000003344 environmental pollutant Substances 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 238000012856 packing Methods 0.000 description 4
- 231100000719 pollutant Toxicity 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 238000004062 sedimentation Methods 0.000 description 4
- 239000005416 organic matter Substances 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 102000040350 B family Human genes 0.000 description 2
- 108091072128 B family Proteins 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 241001269238 Data Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 229910017313 Mo—Co Inorganic materials 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- JYIBXUUINYLWLR-UHFFFAOYSA-N aluminum;calcium;potassium;silicon;sodium;trihydrate Chemical compound O.O.O.[Na].[Al].[Si].[K].[Ca] JYIBXUUINYLWLR-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007809 chemical reaction catalyst Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 229910001603 clinoptilolite Inorganic materials 0.000 description 1
- 239000003426 co-catalyst Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000003403 water pollutant Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/10—Packings; Fillings; Grids
- C02F3/105—Characterized by the chemical composition
- C02F3/108—Immobilising gels, polymers or the like
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/10—Packings; Fillings; Grids
- C02F3/105—Characterized by the chemical composition
- C02F3/107—Inorganic materials, e.g. sand, silicates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Materials Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of pre- film fluidisation bed fillers and the preparation method and application thereof, a kind of pre- film fluidisation bed fillers, comprising: filler material core, specific surface area are greater than 600m2/m3, specific gravity is between 0.95~0.98；Pre- membrane micro layer is coated on filler material wicking surface, from inside to outside at least successively includes two different microorganism colonization layers, wherein nitrobacteria biofilm layer is located on the inside of actinomyces biofilm layer.The present invention is coated with the different microorganism colonization layer of multilayer in filler material wicking surface for the first time, and nitrobacteria biofilm layer is located on the inside of actinomyces biofilm layer, nitrobacteria efficient catalytic nitration reaction under aerobic conditions, is nitrate nitrogen by mineralized nitrogen；Actinomyces provide powerful anti-adversity ability using itself a large amount of mycelium, and the activity of microbial inoculum is protected during filler storage.
Description
Technical field
The present invention relates to a kind of pre- film fluidisation bed fillers and the preparation method and application thereof, belong to technical field of sewage.
Background technique
Biological fluidized bed technology utilization in sewage treatment is attached to the biomembrane purification sewage on floating stuffing, biology
Amount is 2~3 times of conventional activated sludge process, removes organic matter, the efficiency of total nitrogen is also much higher than conventional activated sludge process.Using
The occupied area and operating cost of municipal sewage plant can be significantly reduced in MBBR technique, is getting growing concern for
And application.
The core equipment of MBBR technique is the fluidisation bed fillers that various function bacteriums sufficiently grow attachment, makes the general of COD of degrading
The major functions bacterium such as logical heterotroph, the nitrobacteria for removing total nitrogen and denitrifying bacteria are quickly enough to be attached to filler surface
(biofilm) is the main task using the sewage plant debugging stage of MBBR technique.Biofilm process is usually relatively slower, especially generation
For time longer nitrobacteria, abundant biofilm may be needed up to 4~6 months.This not only cause debugging cycle it is too long,
Water outlet for a long time can not be up to standard, but will medicament during unnecessary consumption debugging, artificial and power cost.
Existing technology relevant to MBBR filler, which is mainly focused on, compares table by the complicated cross sectional shape increase filler of manufacture
Area improves filler hydrophily and bioaffinity, shortens the biofilm period.Chinese patent literature CN103708619A (application number
201410027694.8) a kind of fluidized bed bio film filler preparation method and filling-material structure are disclosed, manufacture complex section is passed through
Shape increases the specific surface area of filler, has strong good hydrophilic property, mithridatism, easy to be filmed, the easily features such as fluidisation.The technology is to removal
The biofilm speed of the general heterotrophic bacteria of COD has certain promotion, but to the generation cycles such as nitrobacteria, nitrite bacteria length
Strain effect is faint, and this kind of flora still needs to the several months could abundant biofilm.
If a kind of organism fluidization bed fillers of preparatory biofilm can be provided, on its surface, enough attachments remove COD, ammonia in advance
Special efficacy flora needed for nitrogen, total nitrogen simultaneously guarantees its activity, so that these fillers is added and works, then can substantially shorten tune
The period is tried, can not only make to be discharged quickly reduction disposal of pollutants up to standard, can more save a large amount of debugging costs.But due to various in flora
The growth cycle and condition of microorganism be not identical, therefore how to ensure the effective quantity of biofilm microorganism, becomes main technology
Problem.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of pre- film fluidisation bed fillers and the preparation method and application thereof.It answers
It is attached to largely remove the functional flora of COD, ammonia nitrogen and total nitrogen in advance with filler prepared by this method, can throw and use, and
And vitamin necessary to being grown rich in a large amount of microorganisms, work is greatly facilitated to the biomembrane activity during follow-up operation
With.
Technical solution of the present invention is as follows:
A kind of pre- film fluidisation bed fillers, comprising:
Filler material core, specific surface area are greater than 600m2/m3, specific gravity is between 0.95~0.98；
Pre- membrane micro layer is coated on filler material wicking surface, from inside to outside at least successively includes two different microorganisms
Biofilm layer, wherein nitrobacteria biofilm layer is located on the inside of actinomyces biofilm layer；Effective viable bacteria amount of nitrobacteria biofilm layer be 2~
7×1011A/L, effective viable bacteria amount of actinomyces biofilm layer are 1~10 × 1013/L。
Preferred according to the present invention, the specific gravity of the pre- film fluidisation bed fillers is not more than 1.Specific gravity can ensure that pre- no more than 1
Film fluidisation bed fillers are in suspended state in water.
Preferred according to the present invention, filler material wicking surface has hydrophily, and the angle θ is not more than 80 °.Hydrophily is higher, pre- film
Period is smaller.
Preferred according to the present invention, it is parts by weight that filler material core, which includes following component:
The lightweight skeleton is selected from polyethylene or polypropylene；
The hard slow release carbon source be selected from polylactic acid (PLA), polyhydroxyalkanoates (PHA), starch plastic, polyadipate/
The mixing of one of butylene terephthalate copolymer (PBAT) or two or more any ratios.
According to the present invention it is further preferred that the ammonia nitrogen pregnant solution is zeolite powder.It is according to the present invention it is furthermore preferred that described
200~400 mesh of zeolite powder partial size, ammonia nitrogen absorption capacity be greater than 1.2mmol/g, density be 1.9~2.4kg/L.
Zeolite powder can improve nitrification by increasing nitrifying process reactant concentration in filler surface adsorpting aggregation ammonia nitrogen
Reaction rate 10%~20%, ammonia nitrogen removal is more thorough.Zeolite powder is by modenite, clinoptilolite, rib zeolite through naturally de-
The process combinings such as water, drying, micronized handles, crush, powder processed, pretreatment molding are process.
According to the present invention it is further preferred that the phosphorus pregnant solution in molysite and/or aluminium salt one or two with
On combination.According to the present invention it is furthermore preferred that the phosphorus pregnant solution is iron chloride；Optimal, the partial size of the iron chloride is
100~200 mesh.
Iron chloride can precipitate aggregation phosphate in filler surface, so that the microorganism for filler surface enough provides proliferation
P elements necessary to being metabolized promote biomembrane growth and biological enzyme secretion, the final removal for strengthening water pollutant.
According to the present invention it is further preferred that the biological enzyme activity increase agent be selected from one of molysite, magnesium salts, manganese salt or
Two or more combinations；According to the present invention it is furthermore preferred that the biological enzyme activity increase agent is iron oxide, magnesium hydroxide and titanium dioxide
The mixture of manganese；Optimal, it is mass percent: iron oxide 40~45%, hydrogen that the biological enzyme activity increase agent component is as follows
Magnesia 20~25%, manganese dioxide 32~36%.
The effect of biological enzyme activity increase agent is to improve the metabolic activity of microorganism in filler surface biomembrane, increase biological enzyme
Secretory volume, that is, increase anti-nitration reaction catalyst yield, to improve denitrification rate and nitric efficiency.
According to the present invention it is further preferred that the lightweight skeleton is polypropylene.
Lightweight skeleton is high-molecular organic material, and density is less than water, plays the role of skeleton and adjusts filler global density,
Final filler specific gravity is between 0.89~0.98.
According to the present invention it is further preferred that 0.3~0.5dL/g of intrinsic viscosity of the polylactic acid, density 1.2~
1.3kg/L。
According to the present invention it is further preferred that 1.2~1.3kg/L of density of the polyhydroxyalkanoates, molecular weight are
500000~1,000,000,110 DEG C~130 DEG C of fusing point.
According to the present invention it is further preferred that the hard slow release carbon source be polylactic acid and polyhydroxyalkanoates in mass ratio
(2~4): 1.
Hard slow release carbon source is more slightly higher than skeleton material hardness, can form salient point in filler surface, identical in cross sectional shape
Under the conditions of, increase packing specific area 15%~20%；Hard slow release carbon source can be used as reducing agent by denitrifying microorganism, pass through
Synchronous nitration and denitrification effect removing total nitrogen；Hard slow release carbon source forms hole point after being depleted, in filler surface, still can be
Cross sectional shape under the same conditions, increases packing specific area 15%~20%.
Preferred according to the present invention, the pre- membrane micro layer is from inside to outside successively comprising bacillus megaterium, photosynthetic thin
Bacterium, bacillus subtilis, nitrobacteria and actinomyces layer.
The effect of above-mentioned bacillus megaterium is dissolution phosphorus deposit, and sufficient phosphorus source is provided for subsequent pre- film；Light and thin
The effect of bacterium is to carry out anabolism using ammonia nitrogen, organic matter under aerobic conditions, and assimilation ammonia nitrogen synthesizes multivitamin,
Enhance microbial activity；The effect of bacillus subtilis is secretion multiple protein enzyme, amylase, lipase, cellulase, differentiation
Disintegrate larger molecular organics and synthesize a variety of B family vitamins, enhances microbial activity；The effect of nitrobacteria is in aerobic conditions
Mineralized nitrogen is nitrate nitrogen by lower efficient catalytic nitration reaction；The effect of actinomyces is attached to composite bacteria agent outermost layer, using certainly
The a large amount of mycelium of body provide that powerful resist drying ability is superpower, and the activity of microbial inoculum is protected during filler storage.
It is further preferred that the bacillus megaterium is the huge of CGMCC number 1.16094,1.10466 or 1.8802
Bacillus strain；
It is further preferred that the photosynthetic bacteria is the hydrogenlike silicon ion of CGMCC number 1.5028 or 1.3368, or
The Rhodospirillum rubrum bacterial strain that CGMCC number is 1.3369；
It is further preferred that the bacillus subtilis is the withered of CGMCC number 1.15792,1.14985 or 1.12938
Careless Bacillus strain；
It is further preferred that the nitrobacteria is that Novi believes 5805 bacteria preparations；
It is further preferred that the actinomyces are the dark orange Actinoplanes bacteria strain of CGMCC number 4.6857, CGMCC
The Li Guliya Actinoplanes bacteria strain of number 4.5521, the Xinjiang plant actinomycetes strain of CGMCC number 4.4663.
The preparation method of above-mentioned pre- film fluidisation bed fillers, includes the following steps:
(1) filler material core is placed in bacillus megaterium bacterium solution, 18~22h is cultivated under the conditions of 28~32 DEG C, be made
The filler of area load bacillus megaterium；
(2) filler of area load bacillus megaterium made from step (1) is placed in photosynthetic bacteria liquid, 28~
Under the conditions of 32 DEG C, control dissolved oxygen concentration cultivates 3~6d in 0.5~1mg/L, and the filler of area load photosynthetic bacteria is made；
(3) filler of area load photosynthetic bacteria made from step (2) is placed in bacillus subtilis bacterium solution, 32~
12~18h is cultivated under the conditions of 38 DEG C, and the filler of area load bacillus subtilis is made；
(4) filler of area load bacillus subtilis made from step (3) is placed in nitrobacteria bacterium solution, 30~
Under the conditions of 36 DEG C, dissolved oxygen concentration cultivates 2~3d in 2~4mg/L pH6.5~8.0, replaces bacterium solution, repeats above-mentioned incubation
4~6 times, the filler of area load nitrobacteria is made；
(5) filler of area load nitrobacteria made from step (4) is placed in actinomyces bacterium solution, in 25~35 DEG C of items
Under part, control dissolved oxygen concentration cultivates 2~3d in 0.5~2mg/L, and the filler of area load actinomyces is made；
(6) filler of area load actinomyces made from step (5) is dried, pre- film fluidisation bed fillers is made.
Preferred according to the present invention, in the step (1), filler material core is to mix raw material in proportion, stirs evenly, adds
Heat is prepared into diameter 22~28mm cylindrical shape filler bar to 180~190 DEG C；It is then cut into the filler of 5~10mm of length
Grain.
Preferred according to the present invention, in the step (1), the cell concentration of bacillus megaterium bacterium solution is 8~12g/L；
Preferably, in the step (1), bacillus megaterium bacterium solution is to be obtained using the culture medium fermented and cultured of following component:
9~11g/L of lactose, peptone 7.2~8g/L, NaCl 4~5g/L, MgSO4.7H2O 2.46g/L、CaCl2
1.22g/L、K2SO40.087g/L, pH8.0.
Preferred according to the present invention, in the step (1), 60%~100% filling MBBR is anti-by volume for filler material core
Answer device.
Preferred according to the present invention, in the step (2), the cell concentration of photosynthetic bacteria liquid is 25~35g/L；It is preferred that
, in the step (2), photosynthetic bacteria liquid is to be obtained using the culture medium fermented and cultured of following component:
NaAc·3H23~3.5g/L of O, glucose 4~4.5g/L, NH4Cl 0.05g/L, pH7.0.
It is preferred according to the present invention, in the step (2), the filler of area load bacillus megaterium by volume 60%
~100% filling MBBR reactor.
Preferred according to the present invention, in the step (3), the cell concentration of bacillus subtilis bacterium solution is 8~12g/L；
Preferably, in the step (3), bacillus subtilis bacterium solution is to be obtained using the culture medium fermented and cultured of following component:
12~16g/L of peptone, glucose 18~21g/L, NaCl 5g/L, beef extract 0.5g/L, agar 20g/L.
It is preferred according to the present invention, in the step (3), the filler of area load photosynthetic bacteria by volume 60%~
100% filling MBBR reactor.
Preferred according to the present invention, in the step (4), the cell concentration of nitrobacteria bacterium solution is 45~55g/L；It is preferred that
, in the step (4), nitrobacteria bacterium solution is to be obtained using the culture medium fermented and cultured of following component:
NH4Cl 3.5~4g/L, pH 6~8.
It is preferred according to the present invention, in the step (4), the filler of area load bacillus subtilis by volume 60%
~100% filling MBBR reactor.
Preferred according to the present invention, in the step (5), the cell concentration of actinomyces bacterium solution is 45~55g/L；It is preferred that
, in the step (5), nitrobacteria bacterium solution is to be obtained using Gause I culture medium fermented and cultured；
It is preferred according to the present invention, in the step (5), the filler of area load nitrobacteria by volume 60%~
100% filling MBBR reactor.
Beneficial effect
1, the present invention is coated with the different microorganism colonization layer of multilayer, nitrobacteria biofilm layer in filler material wicking surface for the first time
On the inside of actinomyces biofilm layer, mineralized nitrogen is nitrate nitrogen by nitrobacteria efficient catalytic nitration reaction under aerobic conditions；It puts
Line bacterium provides powerful anti-adversity ability using itself a large amount of mycelium, and the activity of microbial inoculum is protected during filler storage；
2, when pre- membrane micro layer successively includes bacillus megaterium, photosynthetic bacteria, bacillus subtilis, nitre from inside to outside
When changing bacterium and actinomyces layer, bacillus megaterium can dissolve the phosphorus deposit of filler material wicking surface, provide for subsequent pre- film
Sufficient phosphorus source；The effect of light and bacterium is to carry out anabolism, assimilation ammonia using ammonia nitrogen, organic matter under aerobic conditions
Nitrogen synthesizes multivitamin, enhances microbial activity；The effect of bacillus subtilis is secretion multiple protein enzyme, amylase, rouge
Fat enzyme, cellulase divide and disintegrate larger molecular organics and synthesize a variety of B family vitamins, enhance microbial activity；In conjunction with outer layer
Nitrobacteria biofilm layer and actinomyces biofilm layer, may be implemented to add the wastewater treatment efficiency to work, but work as pre- biofilm
After microbe species replacement or position change in layer, wastewater treatment efficiency is remarkably decreased.
Specific embodiment
Technical solution of the present invention is further elaborated below with reference to embodiment, but institute's protection scope of the present invention is not limited to
This.
It is microbe-derived
Bacillus megaterium is purchased from China General Microbiological culture presevation administrative center, strain CGMCC number 1.16094；
Photosynthetic bacteria is purchased from China General Microbiological culture presevation administrative center, strain CGMCC number 1.5028；
Bacillus subtilis is purchased from China General Microbiological culture presevation administrative center, strain CGMCC number 1.15792；
Nitrobacteria believes (Shenyang) Bioisystech Co., Ltd, product number 5805 purchased from Novi；
Actinomyces are purchased from China General Microbiological culture presevation administrative center, strain CGMCC number 4.6857；
Embodiment 1
A kind of pre- film fluidisation bed fillers, comprising:
Filler material core, specific surface area about 650m2/m3, filler material wicking surface is with hydrophily, and about 75 ° of the angle θ, concrete component is such as
Shown in following table:
The preparation method of above-mentioned pre- film fluidisation bed fillers, includes the following steps:
(1) filler material core is placed in bacillus megaterium bacterium solution, 18~22h is cultivated under the conditions of 28~32 DEG C, be made
The filler of area load bacillus megaterium；
(2) filler of area load bacillus megaterium made from step (1) is placed in photosynthetic bacteria liquid, 28~
Under the conditions of 32 DEG C, control dissolved oxygen concentration cultivates 3~6d in 0.5~1mg/L, and the filler of area load photosynthetic bacteria is made；
(3) filler of area load photosynthetic bacteria made from step (2) is placed in bacillus subtilis bacterium solution, 32~
12~18h is cultivated under the conditions of 38 DEG C, and the filler of area load bacillus subtilis is made；
(4) filler of area load bacillus subtilis made from step (3) is placed in nitrobacteria bacterium solution, 30~
Under the conditions of 36 DEG C, dissolved oxygen concentration cultivates 2~3d in 2~4mg/L pH6.5~8.0, replaces bacterium solution, repeats above-mentioned incubation
4~6 times, the filler of area load nitrobacteria is made；
(5) filler of area load nitrobacteria made from step (4) is placed in actinomyces bacterium solution, in 25~35 DEG C of items
Under part, control dissolved oxygen concentration cultivates 2~3d in 0.5~2mg/L, and the filler of area load actinomyces is made；
(6) filler of area load actinomyces made from step (5) is dried, pre- film fluidisation bed fillers is made.
(7) after draining away the water, it is packaged packing, and is saved backup at shady and cool ventilation.
Embodiment 2
Pre- film fluidizes bed fillers as described in Example 1, the difference is that, the pre- membrane micro layer from inside to outside according to
It is secondary to include nitrobacteria and actinomyces layer.
Embodiment 3
Pre- film fluidizes bed fillers as described in Example 1, the difference is that, the pre- membrane micro layer from inside to outside according to
It is secondary to include photosynthetic bacteria, bacillus megaterium, bacillus subtilis, nitrobacteria and actinomyces layer.
Embodiment 4
Pre- film fluidizes bed fillers as described in Example 1, the difference is that,
Bacillus megaterium is purchased from China General Microbiological culture presevation administrative center, strain CGMCC number 1.10466；
Photosynthetic bacteria is purchased from China General Microbiological culture presevation administrative center, strain CGMCC number 1.3369；
Bacillus subtilis is purchased from China General Microbiological culture presevation administrative center, strain CGMCC number 1.12938；
Nitrobacteria is common Shui nationality nitrobacteria, and online shopping is from Tian Maoxi honor flagship store.
Actinomyces are purchased from China General Microbiological culture presevation administrative center, strain CGMCC number 4.4663；
Embodiment 5
Pre- film fluidizes bed fillers as described in Example 1, the difference is that, the pre- membrane micro layer from inside to outside according to
It is secondary to include photosynthetic bacteria, nitrobacteria, actinomyces layer, bacillus subtilis and bacillus megaterium.
Comparative example 1
Pre- film fluidizes bed fillers as described in Example 1, the difference is that, the pre- membrane micro layer from inside to outside according to
Secondary includes photosynthetic bacteria, actinomyces layer, nitrobacteria, bacillus subtilis and bacillus megaterium.
Comparative example 2
Pre- film fluidizes bed fillers as described in Example 1, the difference is that, filler material core is outstanding for commercially available modified polyurethane
Floating filler, than weighing about 1.05, specific surface area about 400m2/m3。
Experimental example 1
Filler made from the Examples 1 to 5 and comparative example 1~2 of same volume is taken, after saving 6 months, is respectively used to lack
Oxygen/aerobic process MBBR waste water dam device handles the water outlet of certain municipal sewage plant primary sedimentation tank.
Primary sedimentation tank effluent index: pH value 6.5~7.5,18~26 DEG C of temperature, COD concentration 400mg/L, ammonia nitrogen 50mg/L, always
Nitrogen 55mg/L, TP value 4mg/L, DO 2~4mg/L of value,
Experiment condition is as follows: anoxic zone media-filling rate 40%, aerobic zone media-filling rate 50%, and hydraulic detention time lacks
Oxygen area 1.5h, aerobic zone 4h, mixed liquid recycle ratio 200%.Main result is as follows, and effluent index is all that biofilm biomass is stablized
After test.
1 result of table 1- experimental example
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Comparative example 1 | Comparative example 2 | |
| Biofilm biomass stablizes the time | 10d | 18d | 12d | 10d | 16d | 14d | 26d |
| Microbial biomass after biofilm biomass is stablized | 9.74g/L | 8.21g/L | 9.06g/L | 9.72 | 8.79g/L | 8.74g/L | 3.47g/L |
| It is discharged COD (mg/L) | 20.1 | 30.4 | 21.6 | 20.2 | 29.3 | 29.4 | 36.4 |
| It is discharged ammonia nitrogen (mg/L) | 1.0 | 1.5 | 1.1 | 1.2 | 1.3 | 1.8 | 2.1 |
| It is discharged total nitrogen (mg/L) | 5.3 | 6.1 | 5.5 | 5.7 | 5.8 | 6.8 | 6.6 |
| It is discharged total phosphorus (mg/L) | 0.5 | 0.9 | 0.6 | 0.6 | 0.7 | 0.7 | 1.2 |
| COD removal rate (%) | 95.0 | 92.4 | 94.6 | 95.0 | 92.7 | 92.7 | 90.9 |
| Ammonia nitrogen removal frank (%) | 98.0 | 97.0 | 97.8 | 97.6 | 97.4 | 96.4 | 95.8 |
| Nitrogen removal rate (%) | 90.4 | 88.9 | 90.0 | 89.6 | 89.5 | 87.6 | 88.0 |
| Total tp removal rate (%) | 87.5 | 77.5 | 85.0 | 85.0 | 82.5 | 82.5 | 70.0 |
Using the device of pre- film fluidisation bed fillers prepared by embodiment 1, biofilm biomass reaches stable after 10 days, and answers
Apparent biomembrane can't usually occur within 10 days when common MBBR filler；Microbial biomass after biofilm biomass is stablized reaches
To 9.74g/L, and when the common MBBR filler of application, microbial biomass is about in 2.5g/L or so；(about 10 after biofilm biomass is stable
In it), it is discharged COD, ammonia nitrogen, total nitrogen and total phosphorus concentration respectively reach 20.1mg/L, 1.0mg/L, 5.3mg/L and 0.5mg/L, go
Except rate respectively reaches 95.0%, 98.0%, 90.4% and 87.5%, and when the common MBBR filler of application, water outlet reaches stable
Time is about 40~60 days, is discharged COD about 40~50mg/L, ammonia nitrogen about 8~10mg/L, total nitrogen about 15~17mg/L after stablizing.
As it can be seen that can obviously accelerate the speed that MBBR technique reaches stable state using pre- film fluidisation bed fillers provided by the invention, and
Effluent characteristics are significantly improved, so as to shorten debugging process, save debugging cost.
Bed fillers are fluidized using pre- film prepared by embodiment 2, the microbial inoculum type loaded in advance in biomembrane is only to nitrify carefully
Bacterium and actinomyces have lacked bacillus megaterium, photosynthetic bacteria and bacillus subtilis compared with Example 1.It is loaded in advance
Nitrobacteria and actinomyces under the action of, water outlet ammonia nitrogen, total nitrogen index still are able to quickly reduce, and drop respectively at the 18th day
To 1.5mg/L and 6.1mg/L, better than the index in the case of common MBBR filler.But due to lacking bacillus megaterium, photosynthetic
Bacterium and bacillus subtilis, microbial metabolic activity is slightly lower, not strictly according to the facts to the removal efficiency bacterium of COD, ammonia nitrogen, total nitrogen, total phosphorus
Apply example 1.
Adopt pre- film fluidisation bed fillers prepared with embodiment 3, compared to embodiment 1, only have exchanged bacillus megaterium and
The Mo-Co catalyst of photosynthetic bacteria.Compared to embodiment 1, biofilm biomass is stablized the time, microbial biomass and each after biofilm biomass is stablized
Item pollutant index is slightly inferior.This is because bacillus megaterium is attached to the second layer from inside to outside, to phosphorus in filler core
The P elements utilization rate of pregnant solution capture is relatively low, caused by overall microbial metabolic activity is slightly lower.
The pre- film fluidisation bed fillers prepared using embodiment 4 change microbial inoculum source, in biomembrane compared to embodiment 1
It does not make significant difference in terms of microbial biomass, COD, total phosphorus removal effect after amount stablizes the time, biofilm biomass is stablized.But due to nitre
Change bacterium and use common online shopping nitrifier kind, nitrobacteria activity is weaker in pre- film, and ammonia nitrogen and total nitrogen removal effect are slightly worse.
Bed fillers are fluidized using pre- film prepared by embodiment 5, compared to embodiment 1, have adjusted bacillus subtilis and huge
They are placed in outermost layer by the attachment position of Bacterium anthracoides.Since bacillus subtilis and bacillus megaterium are to dry empty
The tolerance of gas is lower than actinomyces, the bacillus subtilis and huge bud that the filler that after saving 6 months prepared by comparative example 1 is loaded
Spore bacillus has part loss, causes it to weaken the invigoration effect of microbial metabolic activity, shows as corresponding device in test
Biofilm biomass, which reaches, stablizes required time longer (16 days), and the microbial biomass after biofilm biomass is stablized is slightly lower, is discharged indices
Concentration is also higher.
Bed fillers are fluidized using pre- film prepared by comparative example 1, compared with Example 1, most important difference is unwrapping wire
Bacterium is placed in the second layer from inside to outside, and by nitrobacteria, bacillus subtilis and bacillus megaterium be sequentially placed into third,
Four, five layers.Due to having lacked actinomyces in outermost degeneration-resistant protective effect, pre- film filler is after saving 60 days, withered grass gemma
Bacillus, bacillus megaterium largely fall off, nitrobacteria mortality.Also therefore, using the device of comparative example 1 in test process
In, there is lower, the especially removal effect of ammonia nitrogen and total nitrogen to the removal rate of every pollutant index, hence it is evident that be lower than experimental example
1-5。
Bed fillers are fluidized using pre- film prepared by comparative example 2, filler core used is common commercially available modified polyurethane filler,
Specific gravity is about 1.05, and specific surface area is about 400m2/m3, not MBBR filler special.Since specific gravity is greater than water, which often gathers
Collection is in experimental rig bottom, and insufficient with waste water, mass transfer is bad；In addition, the packing specific area is smaller, can be attached after stablizing
Microbial biomass it is less, weaker to the purification of sewage, every pollutant index concentration is above other several fillers.But pass through
Using pre- film measure, the overall performance of sewage is purified still better than common MBBR filler.(as previously mentioned, being filled out using common MBBR
When material, it is about 40~60 days that water outlet, which reaches the stable time, is discharged COD about 40~50mg/L, ammonia nitrogen about 8~10mg/ after stablizing
L, total nitrogen about 15~17mg/L.)
Experimental example 2
Filler made from the embodiment 1, embodiment 3 and comparative example 1 of same volume is taken, after being made in 3 days, is respectively used to
Anoxic/aerobic process MBBR waste water dam device handles the water outlet of certain municipal sewage plant primary sedimentation tank (with experimental example 1
It is identical).
Primary sedimentation tank effluent index: pH value 6.5~7.5,18~26 DEG C of temperature, COD concentration 400mg/L, ammonia nitrogen 50mg/L, always
Nitrogen 55mg/L, TP value 4mg/L, DO 2~4mg/L of value,
Experiment condition is as follows: anoxic zone media-filling rate 40%, aerobic zone media-filling rate 50%, and hydraulic detention time lacks
Oxygen area 1.5h, aerobic zone 4h, mixed liquid recycle ratio 200%.Main result is as follows, and effluent index is all that biofilm biomass is stablized
After test.
2 result of table 2- experimental example
In table 2, it is consistent with corresponding embodiment, comparative example in table 1 to save 6 months corresponding test datas；Preservation 3 days right
The test data answered is obtained by addition test.
Using embodiment 1 prepare pre- film fluidize bed fillers, save 3 days and preservation 6 months after compare, the equal energy of biofilm biomass
Reach stable in 10 days.In terms of microbial biomass and effluent index after stabilization, the filler after saving 3 days is slightly good, but differs nothing
It is several.This explanation uses the pre- film filler of scheme preparation of the present invention really can be with long-term preservation, without the bright of function
Aobvious decline.Bed fillers are fluidized using pre- film prepared by embodiment 2, also there is similar test result.
Bed fillers are fluidized using pre- film prepared by comparative example 1, are made in 3 days in use, effect and embodiment 1 are without significant
Difference.But in use, significant degenerate occurs in effect after long-term preservation.This is because actinomyces are in second from inside to outside
Layer, nitrobacteria, bacillus subtilis, the bacillus megaterium of outer layer are protected due to having lacked the resistance of actinomyces,
Have and be largely lost, causes to stablize the side such as time, microbial biomass and contaminant-removal properties in biomembrane in application process
The index in face all has degeneration.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of pre- film fluidizes bed fillers characterized by comprising
Filler material core, specific surface area are greater than 600m2/m3, specific gravity is between 0.95~0.98；
Pre- membrane micro layer is coated on filler material wicking surface, from inside to outside at least successively includes two different microorganism colonizations
Layer, wherein nitrobacteria biofilm layer is located on the inside of actinomyces biofilm layer；Effective viable bacteria amount of nitrobacteria biofilm layer be 2~7 ×
1011A/L, effective viable bacteria amount of actinomyces biofilm layer are 1~10 × 1013/L。
2. pre- film as described in claim 1 fluidizes bed fillers, which is characterized in that the specific gravity of the pre- film fluidisation bed fillers is little
In 1；
Preferably, filler material wicking surface has hydrophily, and the angle θ is not more than 80 °；
Preferably, filler material core includes following component, is parts by weight:
The lightweight skeleton is selected from polyethylene or polypropylene；
The hard slow release carbon source is selected from polylactic acid (PLA), polyhydroxyalkanoates (PHA), starch plastic, polyadipate/to benzene
The mixing of one of two ester copolymer of dioctyl phthalate fourth (PBAT) or two or more any ratios.
3. pre- film as claimed in claim 2 fluidizes bed fillers, which is characterized in that the ammonia nitrogen pregnant solution is zeolite powder；According to
The present invention is it is furthermore preferred that 200~400 mesh of zeolite powder partial size, ammonia nitrogen absorption capacity are greater than 1.2mmol/g, density 1.9
~2.4kg/L；
It is further preferred that phosphorus pregnant solution a combination of one or more in molysite and/or aluminium salt；According to this
Invention is it is furthermore preferred that the phosphorus pregnant solution is iron chloride；Optimal, the partial size of the iron chloride is 100~200 mesh；
It is further preferred that the biological enzyme activity increase agent is selected from the group of one or more of molysite, magnesium salts, manganese salt
It closes；According to the present invention it is furthermore preferred that the biological enzyme activity increase agent is the mixture of iron oxide, magnesium hydroxide and manganese dioxide；
Optimal, it is mass percent that the biological enzyme activity increase agent component is as follows: iron oxide 40~45%, magnesium hydroxide 20~
25%, manganese dioxide 32~36%.
It is further preferred that the lightweight skeleton is polypropylene；
It is further preferred that 0.3~0.5dL/g of intrinsic viscosity of the polylactic acid, 1.2~1.3kg/L of density；
It is further preferred that 1.2~1.3kg/L of density of the polyhydroxyalkanoates, molecular weight is 500,000~1,000,000, is melted
110 DEG C~130 DEG C of point；
It is further preferred that the hard slow release carbon source is polylactic acid and polyhydroxyalkanoates (2~4) in mass ratio: 1.
4. pre- film as described in claim 1 fluidizes bed fillers, which is characterized in that the pre- membrane micro layer is from inside to outside successively
Include bacillus megaterium, photosynthetic bacteria, bacillus subtilis, nitrobacteria and actinomyces layer.
5. pre- film as claimed in claim 4 fluidizes bed fillers, which is characterized in that the bacillus megaterium is CGMCC number
1.16094,1.10466 or 1.8802 bacillus megaterium bacterial strain；
It is further preferred that the photosynthetic bacteria is that the hydrogenlike silicon ion of CGMCC number 1.5028 or 1.3368 or CGMCC are compiled
Number be 1.3369 Rhodospirillum rubrum bacterial strain；
It is further preferred that the bacillus subtilis is the withered grass bud of CGMCC number 1.15792,1.14985 or 1.12938
Spore bacillus strain；
It is further preferred that the nitrobacteria is that Novi believes 5805 bacteria preparations；
It is further preferred that the actinomyces are the dark orange Actinoplanes bacteria strain of CGMCC number 4.6857, CGMCC number
4.5521 Li Guliya Actinoplanes bacteria strain, the Xinjiang plant actinomycetes strain of CGMCC number 4.4663.
6. the preparation method of pre- film fluidisation bed fillers described in claim 1, which comprises the steps of:
(1) filler material core is placed in bacillus megaterium bacterium solution, 18~22h is cultivated under the conditions of 28~32 DEG C, surface is made
Load the filler of bacillus megaterium；
(2) filler of area load bacillus megaterium made from step (1) is placed in photosynthetic bacteria liquid, at 28~32 DEG C
Under the conditions of, control dissolved oxygen concentration cultivates 3~6d in 0.5~1mg/L, and the filler of area load photosynthetic bacteria is made；
(3) filler of area load photosynthetic bacteria made from step (2) is placed in bacillus subtilis bacterium solution, at 32~38 DEG C
Under the conditions of cultivate 12~18h, be made area load bacillus subtilis filler；
(4) filler of area load bacillus subtilis made from step (3) is placed in nitrobacteria bacterium solution, at 30~36 DEG C
Under the conditions of, dissolved oxygen concentration cultivates 2~3d in 2~4mg/L pH6.5~8.0, replaces bacterium solution, repeats above-mentioned incubation 4~6
It is secondary, the filler of area load nitrobacteria is made；
(5) filler of area load nitrobacteria made from step (4) is placed in actinomyces bacterium solution, in 25~35 DEG C of conditions
Under, control dissolved oxygen concentration cultivates 2~3d in 0.5~2mg/L, and the filler of area load actinomyces is made；
(6) filler of area load actinomyces made from step (5) is dried, pre- film fluidisation bed fillers is made.
7. preparation method as claimed in claim 6, which is characterized in that in the step (1), filler material core is by raw material
It mixes, stirs evenly in proportion, be heated to 180~190 DEG C, be prepared into diameter 22~28mm cylindrical shape filler bar；Then it cuts
At the filler particles of 5~10mm of length；
Preferably, in the step (1), the cell concentration of bacillus megaterium bacterium solution is 8~12g/L；Preferably, the step
(1) in, bacillus megaterium bacterium solution is to be obtained using the culture medium fermented and cultured of following component:
9~11g/L of lactose, peptone 7.2~8g/L, NaCl 4~5g/L, MgSO4.7H2O 2.46g/L、CaCl2 1.22g/
L、K2SO40.087g/L, pH8.0；
Preferably, in the step (1), filler material core 60%~100% fills MBBR reactor by volume.
8. preparation method as claimed in claim 6, which is characterized in that in the step (2), the thallus of photosynthetic bacteria liquid is dense
Degree is 25~35g/L；Preferably, in the step (2), photosynthetic bacteria liquid is the culture medium fermented and cultured using following component
It obtains:
NaAc·3H23~3.5g/L of O, glucose 4~4.5g/L, NH4Cl 0.05g/L, pH7.0；
Preferably, in the step (2), 60%~100% filling by volume of the filler of area load bacillus megaterium
MBBR reactor.
9. preparation method as claimed in claim 6, which is characterized in that in the step (3), the bacterium of bacillus subtilis bacterium solution
Bulk concentration is 8~12g/L；Preferably, in the step (3), bacillus subtilis bacterium solution is the culture medium using following component
Fermented and cultured obtains:
12~16g/L of peptone, glucose 18~21g/L, NaCl 5g/L, beef extract 0.5g/L, agar 20g/L；
Preferably, in the step (3), 60%~100% filling MBBR is anti-by volume for the filler of area load photosynthetic bacteria
Answer device.
10. preparation method as claimed in claim 6, which is characterized in that in the step (4), the thallus of nitrobacteria bacterium solution
Concentration is 45~55g/L；Preferably, in the step (4), nitrobacteria bacterium solution is the culture medium fermentation training using following component
It supports and obtains:
NH4Cl 3.5~4g/L, pH 6~8；
Preferably, in the step (4), 60%~100% filling by volume of the filler of area load bacillus subtilis
MBBR reactor；
Preferably, in the step (5), the cell concentration of actinomyces bacterium solution is 45~55g/L；Preferably, the step (5)
In, nitrobacteria bacterium solution is to be obtained using Gause I culture medium fermented and cultured；
Preferably, in the step (5), 60%~100% filling MBBR is anti-by volume for the filler of area load nitrobacteria
Answer device.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115043486A (en) * | 2022-05-16 | 2022-09-13 | 中国长江三峡集团有限公司 | Method for deep denitrification by using modified zeolite |
| CN115417508A (en) * | 2022-09-16 | 2022-12-02 | 江苏华淼生态科技有限公司 | Efficient bottom sediment microbial repairing agent and preparation method thereof |
| CN115504818A (en) * | 2022-10-11 | 2022-12-23 | 白水县盛隆果业有限责任公司 | Irrigation fertilizer for improving apple yield |
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| US4322296A (en) * | 1980-08-12 | 1982-03-30 | Kansas State Univ. Research Foundation | Method for wastewater treatment in fluidized bed biological reactors |
| CN104030457A (en) * | 2014-05-21 | 2014-09-10 | 东莞市华中生物科技有限公司 | Method and fluidized bed for purifying eutrophic water by using microorganism filler |
| CN105347477A (en) * | 2015-11-13 | 2016-02-24 | 南京工业大学 | Bionic fluidization carrier for wastewater treatment biological fluidized bed and preparation method and application thereof |
-
2019
- 2019-02-25 CN CN201910138237.9A patent/CN109607764B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4322296A (en) * | 1980-08-12 | 1982-03-30 | Kansas State Univ. Research Foundation | Method for wastewater treatment in fluidized bed biological reactors |
| CN104030457A (en) * | 2014-05-21 | 2014-09-10 | 东莞市华中生物科技有限公司 | Method and fluidized bed for purifying eutrophic water by using microorganism filler |
| CN105347477A (en) * | 2015-11-13 | 2016-02-24 | 南京工业大学 | Bionic fluidization carrier for wastewater treatment biological fluidized bed and preparation method and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115043486A (en) * | 2022-05-16 | 2022-09-13 | 中国长江三峡集团有限公司 | Method for deep denitrification by using modified zeolite |
| CN115417508A (en) * | 2022-09-16 | 2022-12-02 | 江苏华淼生态科技有限公司 | Efficient bottom sediment microbial repairing agent and preparation method thereof |
| CN115504818A (en) * | 2022-10-11 | 2022-12-23 | 白水县盛隆果业有限责任公司 | Irrigation fertilizer for improving apple yield |
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