CN109601254A - A kind of Antrodia camphorata cultural method that cultured products are kept completely separate with matrix - Google Patents
A kind of Antrodia camphorata cultural method that cultured products are kept completely separate with matrix Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention provides the Antrodia camphorata cultural methods that a kind of cultured products and matrix are kept completely separate, it selects cereal to be mixed with cinnamomum kanehirai sawdust as culture substrate, the mixing of different cereal is placed on lower layer, a small amount of cinnamomum kanehirai sawdust is laid on thereon, when inoculation in the medium between make a call to a Column groove, by strain block access wherein.After the completion of culture, culture substrate surface forms one layer of densification and has certain thickness subiculum, which can remove easily, while the mycelium for being similar to stem is formed in Column groove, its top half is sampled, remaining mycelium can also continue to grow.Utilize this method, the present invention not only solves plate method cultured products more difficult the problem of being kept completely separate with matrix, the sawdust being mixed in cultured products can also be removed and promote its yield and triterpenes components content, practice in terms of for Antrodia camphorata solid state fermentation culture provides reference Help, it also provides the foundation simultaneously for subsequent large scale fermentation culture, low in cost, operation is simply.
Description
Technical field
The present invention relates to a kind of breeding methods of Antrodia camphorata, refer in particular to a kind of Antrodia camphorata that cultured products are kept completely separate with matrix
Cultural method.
Background technique
Antrodia camphorata (Antrodia camphorata) belong to Polyporaceae, antrodia karst, it is TaiWan, China distinctive one
The rare medicinal fungi of kind.In June, 2016, General Office, the People's Government, Guangdong Province printed and distributed " protection of Guangdong Province's promotion Chinese medicine and development
The notice of embodiment (2016-2020) " [ Yue Fu does (2016) 61 ], by Antrodia camphorata be classified as reinforce Chinese material medicine resource protection,
Rare Chinese medicine in imminent danger is built to plant cultivation base, carry out 20 kinds of Special Traditional Chinese Medicine resource assessments and breeding laid special stress on protecting and developed
One of kind of technical research.The mycelium and fructification of Antrodia camphorata physiological activator rich in, including triterpenes
Compound, polysaccharide, maleic acid and succinic acid derivative etc..Modern scholar the study found that Antrodia camphorata not only to caused by chemically
Hepatic injury has protective effect, while also having the effects that anticancer, anti-oxidant, anti-inflammatory and immunological regulation.
Antrodia camphorata obvious effect, market demand increase severely, and wild Antrodia camphorata slow growth, yield is extremely low, and Antrodia camphorata
Unique host's cinnamomum kanehirai is national child care class tree species, therefore now mostly uses the mode culture Antrodia camphorata of artificial culture.Manually cultivate
Method mainly has linden cultivation, plate method, liquid culture method.Linden cultivation growth cycle is long, and quality is more difficult to control,
But it can cultivate to obtain and Antrodia camphorata fructification similar in wild Antrodia camphorata ingredient；Liquid culture method cultivation cycle is short, and yield is high, but
Cultured products active constituent content is lower, can not cultivate to obtain Antrodia camphorata fructification；Plate method growth cycle is moderate, quality
Controllably, Antrodia camphorata fructification similar in available and wild Antrodia camphorata ingredient when culture reaches certain time, but cultured products are past
It is separated toward more difficult with matrix.
Summary of the invention
It is an object of the invention to be directed to the existing state of the art, it is good to provide a kind of low cost, simple process, yield and quality,
And the Antrodia camphorata cultural method that cultured products and matrix can be made to be kept completely separate.
In order to achieve the above objectives, the present invention adopts the following technical scheme:
The present invention is the Antrodia camphorata cultural method that a kind of cultured products and matrix are kept completely separate, and is mainly comprised the steps that
(1) activation inoculation raw material: take 4 DEG C preservation Antrodia camphorata strains after activation culture 15-30d on PDA plate culture medium
It is punched close to subiculum edge, punch diameter is 0.8cm, obtains mycelia block for being inoculated with；
(2) it prepares solid-state fermentation culture medium: weighing a certain amount of cereal materials and cinnamomum kanehirai sawdust is put in culture bottle, cereal is former
Material, which mixes, is placed on culture bottle lower layer, and cinnamomum kanehirai sawdust tiles thereon；Separately by glucose, KH2PO4、MgSO4·7H2O is dissolved in
A certain amount of distilled water forms the solution containing glucose and inorganic salts, then pours this solution into and impregnates solids in culture bottle
Material, makes the final water content 50%-70% of culture medium, soaking time 2h be then placed in high-pressure sterilizing pot and sterilize, sterilizing temperature
Degree is 121 DEG C, time 30min；
(3) punching inoculation: the cylindrical groove of 2cm or so depth is made a call in solid medium center with glass bar, by raw material
The one side that mycelia block contains mycelia is seeded in cylindrical slot close to culture substrate, is placed in 28 DEG C of dark trainings in constant incubator
It supports；
(4) sterile super-clean bench sampling: after a certain period of time, being formed in stromal surface can easily remove with a thickness of 3-5cm or so for culture
Fine and close subiculum, sampled in sterile super-clean bench, remaining mycelium can also continue to grow in culture medium.
In above scheme, the processing method of cinnamomum kanehirai sawdust is to be placed in after outdoor is exposed to the sun to be sterilized with high-pressure sterilizing pot, is sterilized
Temperature is 121 DEG C, time 2h, and 80 DEG C of drying in baking oven are put it into after sterilizing.
Further, cereal materials are rice 50g, wheat bran 2.5g, are fitted into culture bottle after siccative is mixed, and bottle body is cultivated
Product is 450mL.
Further, cinnamomum kanehirai sawdust is the sheet sawdust that cinnamomum kanehirai timber is ground into 0.5cm or so, weighs drying
Cinnamomum kanehirai sawdust 0.5-7g is laid in cereal stromal surface.
Further, in the solution containing glucose and inorganic salts, glucose 5g, KH2PO4For 0.5g, MgSO4·
7H2O is 0.5g.
The invention has the benefit that cereal is selected to be mixed with cinnamomum kanehirai sawdust as culture substrate, which is both ox
Antrodia growth provides necessary nutriment, and its required cinnamomum kanehirai sawdust in field grown is added, make culture medium at
Divide more comprehensively.Selecting moisture content in medium later is 70%, with the raising of moisture content in medium, the growth of antrodia mycelia
Gradually tend to accumulate in stromal surface, mycelia no longer grows into culture medium, to make mycelium make full use of the battalion in culture medium
Support, when inoculation in the medium between punching form a cylindrical groove, strain block is accessed wherein.After the completion of culture, culture
Stromal surface forms one layer of densification and has certain thickness subiculum, which can remove from matrix easily, while in cylinder
Mycelia, which combines, in shape slot forms the mycelium for being similar to stem, its top half is sampled, remaining mycelium can also continue to
It is grown in matrix.This method can harvest a large amount of cultured products in stromal surface, and cultured products freeze do after sampling
It is dry, it is mixed with a small amount of sawdust in obtained bacteria cake, can be easily removed.
Detailed description of the invention:
Attached drawing 1 is Antrodia camphorata growing state (50d) on different moisture content basal medium, from left to right water content be followed successively by 50%,
60%,70%；
Attached drawing 2 is to add Antrodia camphorata growing state (60d) on different cinnamomum kanehirai sawdust mediums, the cinnamomum kanehirai sawdust addition of 1-5
Amount is respectively 0.5g/ bottles, 1g/ bottles, 3g/ bottles, 5g/ bottles, 7g/ bottles；
Attached drawing 3 is the Antrodia camphorata grown in culture bottle, and the left side is grain culture medium, and the right is that cereal adds sawdust medium；
Attached drawing 4 is the Antrodia camphorata bacteria cake obtained after being freeze-dried.
Specific embodiment:
Embodiment 1:
A kind of Antrodia camphorata cultural method that cultured products are kept completely separate with matrix, mainly comprises the steps that
(1) activation inoculation raw material: take the Antrodia camphorata strain of 4 DEG C of preservations after PDA plate culture medium activation culture 15d in punch
It is punched at centre distance subiculum outer edge 0.4cm, obtains mycelia block for being inoculated with；
(2) it prepares solid-state fermentation culture medium: weighing a certain amount of cereal materials and cinnamomum kanehirai sawdust is put in culture bottle, cereal is former
Material is rice 50g, wheat bran 2.5g, is fitted into culture bottle after siccative is mixed, and culture bottle volume is 450mL, and cereal materials mix
It is placed on culture bottle lower layer, cinnamomum kanehirai sawdust tiles thereon；Separately by glucose 5g, KH2PO40.5g, MgSO4·7H2O 0.5g
It is dissolved in a certain amount of distilled water and forms the solution containing glucose and inorganic salts, then pour this solution into culture bottle and impregnate admittedly
Body material makes the final water content 60% of culture medium, and soaking time 2h is then placed in high-pressure sterilizing pot and sterilizes, sterilizing temperature
Degree is 121 DEG C, time 30min；
The processing method of cinnamomum kanehirai sawdust is to be placed in after outdoor is exposed to the sun to be sterilized with high-pressure sterilizing pot, and sterilising temp is 121 DEG C, the time
For 2h, 80 DEG C of drying in baking oven are put it into after sterilizing.Cinnamomum kanehirai sawdust is the piece that cinnamomum kanehirai timber is ground into 0.5cm or so
Shape sawdust, the cinnamomum kanehirai sawdust 3g for weighing drying are laid in cereal stromal surface.
(3) punching inoculation: making a call to the cylindrical groove of 2cm or so depth in solid medium center with glass bar, will
The one side that raw material mycelia block contains mycelia is seeded in cylindrical slot close to culture substrate is placed in constant incubator 28 DEG C
Dark culture；
(4) sterile super-clean bench sampling: after a certain period of time, being formed in stromal surface can easily remove with a thickness of 3-5cm or so for culture
Fine and close subiculum, sampled in sterile super-clean bench, remaining mycelium can also continue to grow in culture medium.
Embodiment 2:
A kind of Antrodia camphorata cultural method that cultured products are kept completely separate with matrix, mainly comprises the steps that
(1) activation inoculation raw material: the Antrodia camphorata strain of 4 DEG C of preservations is taken to punch after activation culture 20d on PDA plate culture medium
It is punched at device centre distance subiculum outer edge 0.4cm, obtains mycelia block for being inoculated with；
(2) it prepares solid-state fermentation culture medium: weighing a certain amount of cereal materials and cinnamomum kanehirai sawdust is put in culture bottle, cereal is former
Material is rice 50g, wheat bran 2.5g, is fitted into culture bottle after siccative is mixed, and culture bottle volume is 450mL, and cereal materials mix
It is placed on culture bottle lower layer, cinnamomum kanehirai sawdust tiles thereon；Separately by glucose 5g, KH2PO40.5g, MgSO4·7H2O 0.5g
It is dissolved in a certain amount of distilled water and forms the solution containing glucose and inorganic salts, then pour this solution into culture bottle and impregnate admittedly
Body material makes the final water content 70% of culture medium, and soaking time 2h is then placed in high-pressure sterilizing pot and sterilizes, sterilizing temperature
Degree is 121 DEG C, time 30min；
The processing method of cinnamomum kanehirai sawdust is to be placed in after outdoor is exposed to the sun to be sterilized with high-pressure sterilizing pot, and sterilising temp is 121 DEG C, the time
For 2h, 80 DEG C of drying in baking oven are put it into after sterilizing.Cinnamomum kanehirai sawdust is the piece that cinnamomum kanehirai timber is ground into 0.5cm or so
Shape sawdust, the cinnamomum kanehirai sawdust 1g for weighing drying are laid in cereal stromal surface.
(3) punching inoculation: making a call to the cylindrical groove of 2cm or so depth in solid medium center with glass bar, will
The one side that raw material mycelia block contains mycelia is seeded in cylindrical slot close to culture substrate is placed in constant incubator 28 DEG C
Dark culture；
(4) sterile super-clean bench sampling: after a certain period of time, being formed in stromal surface can easily remove with a thickness of 3-5cm or so for culture
Fine and close subiculum, sampled in sterile super-clean bench, remaining mycelium can also continue to grow in culture medium.
Embodiment 3:
A kind of Antrodia camphorata cultural method that cultured products are kept completely separate with matrix, mainly comprises the steps that
(1) activation inoculation raw material: the Antrodia camphorata strain of 4 DEG C of preservations is taken to punch after activation culture 25d on PDA plate culture medium
It is punched at device centre distance subiculum outer edge 1.2cm, obtains mycelia block for being inoculated with；
(2) it prepares solid-state fermentation culture medium: weighing a certain amount of cereal materials and cinnamomum kanehirai sawdust is put in culture bottle, cereal is former
Material is rice 50g, wheat bran 2.5g, is fitted into culture bottle after siccative is mixed, and culture bottle volume is 450mL, and cereal materials mix
It is placed on culture bottle lower layer, cinnamomum kanehirai sawdust tiles thereon；Separately by glucose 5g, KH2PO40.5g, MgSO4·7H2O 0.5g
It is dissolved in a certain amount of distilled water and forms the solution containing glucose and inorganic salts, then pour this solution into culture bottle and impregnate admittedly
Body material makes the final water content 50% of culture medium, and soaking time 2h is then placed in high-pressure sterilizing pot and sterilizes, sterilizing temperature
Degree is 121 DEG C, time 30min；
The processing method of cinnamomum kanehirai sawdust is to be placed in after outdoor is exposed to the sun to be sterilized with high-pressure sterilizing pot, and sterilising temp is 121 DEG C, the time
For 2h, 80 DEG C of drying in baking oven are put it into after sterilizing.Cinnamomum kanehirai sawdust is the piece that cinnamomum kanehirai timber is ground into 0.5cm or so
Shape sawdust, the cinnamomum kanehirai sawdust 5g for weighing drying are laid in cereal stromal surface.
(3) punching inoculation: making a call to the cylindrical groove of 2cm or so depth in solid medium center with glass bar, will
The one side that raw material mycelia block contains mycelia is seeded in cylindrical slot close to culture substrate is placed in constant incubator 28 DEG C
Dark culture；
(4) sterile super-clean bench sampling: after a certain period of time, being formed in stromal surface can easily remove with a thickness of 3-5cm or so for culture
Fine and close subiculum, sampled in sterile super-clean bench, remaining mycelium can also continue to grow in culture medium.
Embodiment 4:
A kind of Antrodia camphorata cultural method that cultured products are kept completely separate with matrix, mainly comprises the steps that
(1) activation inoculation raw material: the Antrodia camphorata strain of 4 DEG C of preservations is taken to punch after activation culture 30d on PDA plate culture medium
It is punched at device centre distance subiculum outer edge 1.2cm, obtains mycelia block for being inoculated with；
(2) it prepares solid-state fermentation culture medium: weighing a certain amount of cereal materials and cinnamomum kanehirai sawdust is put in culture bottle, cereal is former
Material is rice 50g, wheat bran 2.5g, is fitted into culture bottle after siccative is mixed, and culture bottle volume is 450mL, and cereal materials mix
It is placed on culture bottle lower layer, cinnamomum kanehirai sawdust tiles thereon；Separately by glucose 5g, KH2PO40.5g, MgSO4·7H2O 0.5g
It is dissolved in a certain amount of distilled water and forms the solution containing glucose and inorganic salts, then pour this solution into culture bottle and impregnate admittedly
Body material makes the final water content 60% of culture medium, and soaking time 2h is then placed in high-pressure sterilizing pot and sterilizes, sterilizing temperature
Degree is 121 DEG C, time 30min；
The processing method of cinnamomum kanehirai sawdust is to be placed in after outdoor is exposed to the sun to be sterilized with high-pressure sterilizing pot, and sterilising temp is 121 DEG C, the time
For 2h, 80 DEG C of drying in baking oven are put it into after sterilizing.Cinnamomum kanehirai sawdust is the piece that cinnamomum kanehirai timber is ground into 0.5cm or so
Shape sawdust, the cinnamomum kanehirai sawdust 0.5g for weighing drying are laid in cereal stromal surface.
(3) punching inoculation: making a call to the cylindrical groove of 2cm or so depth in solid medium center with glass bar, will
The one side that raw material mycelia block contains mycelia is seeded in cylindrical slot close to culture substrate is placed in constant incubator 28 DEG C
Dark culture；
(4) sterile super-clean bench sampling: after a certain period of time, being formed in stromal surface can easily remove with a thickness of 3-5cm or so for culture
Fine and close subiculum, sampled in sterile super-clean bench, remaining mycelium can also continue to grow in culture medium.
Embodiment 5:
A kind of Antrodia camphorata cultural method that cultured products are kept completely separate with matrix, mainly comprises the steps that
(1) activation inoculation raw material: the Antrodia camphorata strain of 4 DEG C of preservations is taken to punch after activation culture 20d on PDA plate culture medium
It is punched at device centre distance subiculum outer edge 0.4cm, obtains mycelia block for being inoculated with；
(2) it prepares solid-state fermentation culture medium: weighing a certain amount of cereal materials and cinnamomum kanehirai sawdust is put in culture bottle, cereal is former
Material is rice 50g, wheat bran 2.5g, is fitted into culture bottle after siccative is mixed, and culture bottle volume is 450mL, and cereal materials mix
It is placed on culture bottle lower layer, cinnamomum kanehirai sawdust tiles thereon；Separately by glucose 5g, KH2PO40.5g, MgSO4·7H2O 0.5g
It is dissolved in a certain amount of distilled water and forms the solution containing glucose and inorganic salts, then pour this solution into culture bottle and impregnate admittedly
Body material makes the final water content 70% of culture medium, and soaking time 2h is then placed in high-pressure sterilizing pot and sterilizes, sterilizing temperature
Degree is 121 DEG C, time 30min；
The processing method of cinnamomum kanehirai sawdust is to be placed in after outdoor is exposed to the sun to be sterilized with high-pressure sterilizing pot, and sterilising temp is 121 DEG C, the time
For 2h, 80 DEG C of drying in baking oven are put it into after sterilizing.Cinnamomum kanehirai sawdust is the piece that cinnamomum kanehirai timber is ground into 0.5cm or so
Shape sawdust, the cinnamomum kanehirai sawdust 7g for weighing drying are laid in cereal stromal surface.
(3) punching inoculation: making a call to the cylindrical groove of 2cm or so depth in solid medium center with glass bar, will
The one side that raw material mycelia block contains mycelia is seeded in cylindrical slot close to culture substrate is placed in constant incubator 28 DEG C
Dark culture；
(4) sterile super-clean bench sampling: after a certain period of time, being formed in stromal surface can easily remove with a thickness of 3-5cm or so for culture
Fine and close subiculum, sampled in sterile super-clean bench, remaining mycelium can also continue to grow in culture medium.
Embodiment 6:
A kind of Antrodia camphorata cultural method that cultured products are kept completely separate with matrix, mainly comprises the steps that
(1) activation inoculation raw material: the Antrodia camphorata strain of 4 DEG C of preservations is taken to punch after activation culture 20d on PDA plate culture medium
It is punched at device centre distance subiculum outer edge 1.2cm, obtains mycelia block for being inoculated with；
(2) it prepares solid-state fermentation culture medium: weighing a certain amount of cereal materials and cinnamomum kanehirai sawdust is put in culture bottle, cereal is former
Material is rice 50g, wheat bran 2.5g, is fitted into culture bottle after siccative is mixed, and culture bottle volume is 450mL, and cereal materials mix
It is placed on culture bottle lower layer, cinnamomum kanehirai sawdust tiles thereon；Separately by glucose 5g, KH2PO40.5g, MgSO4·7H2O 0.5g
It is dissolved in a certain amount of distilled water and forms the solution containing glucose and inorganic salts, then pour this solution into culture bottle and impregnate admittedly
Body material makes the final water content 60% of culture medium, and soaking time 2h is then placed in high-pressure sterilizing pot and sterilizes, sterilizing temperature
Degree is 121 DEG C, time 30min；
The processing method of cinnamomum kanehirai sawdust is to be placed in after outdoor is exposed to the sun to be sterilized with high-pressure sterilizing pot, and sterilising temp is 121 DEG C, the time
For 2h, 80 DEG C of drying in baking oven are put it into after sterilizing.Cinnamomum kanehirai sawdust is the piece that cinnamomum kanehirai timber is ground into 0.5cm or so
Shape sawdust, the cinnamomum kanehirai sawdust 1g for weighing drying are laid in cereal stromal surface.
(3) punching inoculation: making a call to the cylindrical groove of 2cm or so depth in solid medium center with glass bar, will
The one side that raw material mycelia block contains mycelia is seeded in cylindrical slot close to culture substrate is placed in constant incubator 28 DEG C
Dark culture；
(4) sterile super-clean bench sampling: after a certain period of time, being formed in stromal surface can easily remove with a thickness of 3-5cm or so for culture
Fine and close subiculum, sampled in sterile super-clean bench, remaining mycelium can also continue to grow in culture medium.
Compare different activation times (15d, 20d, 25d, 30d), and punching centre distance subiculum edge 0.4cm and
The antrodia mycelia growing way difference of 1.2cm, table 1 specific as follows:
1 Different Activation Methods of table are inoculated with raw material growth 30d growth diameter (cm)
As can be seen from Table 1, using activation culture 20d, at punch center away from subiculum edge 0.4cm(i.e. close to subiculum
Outer edge) at punching obtain inoculation block mode obtained by antrodia mycelia growing way it is best.
It investigates the basal medium (50%, 60%, 70%) of different moisture content and adds various concentration into basal medium
Influence of the cinnamomum kanehirai sawdust (0.5g/ bottles, 1g/ bottles, 3g/ bottles, 5g/ bottles, 7g/ bottles) to Antrodia camphorata growing state, culture is produced
Object is freeze-dried, and the Antrodia camphorata dry weight that every bottle of each group culture medium can be cultivated is compared, specific as shown in Figure 1, Figure 2.
It is optional using the thick triterpene content of vanillic aldehyde-ice acetic acid method measurement cultured products later using ursolic acid as reference substance
The culture medium that Antrodia camphorata yield is high out and triterpenes components content is high, specific such as table 2, table 3:
Antrodia camphorata dry weight and thick triterpene content (50d) on 2 different moisture content basal medium of table
Table 3 adds Antrodia camphorata dry weight and thick triterpene content (60d) on different cinnamomum kanehirai sawdust mediums
Moisture content in medium is 1g/ bottles in 70%, cinnamomum kanehirai sawdust additive amount it can be seen from table 2, table 3, and gained Antrodia camphorata is dry
Weight highest, and the thick triterpene content of cultured products is most.
As shown in figure 3, culture is after a certain period of time, the cause that can easily remove with a thickness of 3-5cm or so is formed in stromal surface
Close subiculum；
As shown in figure 4, the Cinnamomum kanahirai hay of dry weight about 15g can be obtained in every bottle of 90d of culture with being freeze-dried after sampling in sterile super-clean bench
Sesame product.
Certainly, the above is only better embodiments of the invention, and use scope of the invention is not limited with this, therefore, it is all
It is to make equivalent change in the principle of the invention should be included within the scope of the present invention.
Claims (5)
1. the Antrodia camphorata cultural method that a kind of cultured products and matrix are kept completely separate, it is characterised in that: mainly comprise the steps that
(1) activation inoculation raw material: take 4 DEG C preservation Antrodia camphorata strains after activation culture 15-30d on PDA plate culture medium
Punch to obtain mycelia block for being inoculated with close to subiculum edge；
(2) it prepares solid-state fermentation culture medium: weighing a certain amount of cereal materials and cinnamomum kanehirai sawdust is put in culture bottle, cereal is former
Material, which mixes, is placed on culture bottle lower layer, and cinnamomum kanehirai sawdust tiles thereon；Separately by glucose, KH2PO4、MgSO4·7H2O is dissolved in
A certain amount of distilled water forms the solution containing glucose and inorganic salts, then pours this solution into and impregnates solids in culture bottle
Material, makes the final water content 50%-70% of culture medium, soaking time 2h be then placed in high-pressure sterilizing pot and sterilize, sterilizing temperature
Degree is 121 DEG C, time 30min；
(3) punching inoculation: the cylindrical groove of 2cm or so depth is made a call in solid medium center with glass bar, by raw material
The one side that mycelia block contains mycelia is seeded in cylindrical slot close to culture substrate, is placed in 28 DEG C of dark trainings in constant incubator
It supports；
(4) sterile super-clean bench sampling: after a certain period of time, being formed in stromal surface can easily remove with a thickness of 3-5cm or so for culture
Fine and close subiculum, sampled in sterile super-clean bench, remaining mycelium can also continue to grow in culture medium.
2. the Antrodia camphorata cultural method that a kind of cultured products according to claim 1 and matrix are kept completely separate, feature exist
In: the processing method of the cinnamomum kanehirai sawdust is to be placed in after outdoor is exposed to the sun to be sterilized with high-pressure sterilizing pot, sterilising temp 121
DEG C, time 2h puts it into 80 DEG C of drying in baking oven after sterilizing.
3. the Antrodia camphorata cultural method that a kind of cultured products according to claim 1 or 2 and matrix are kept completely separate, feature
Be: the cereal materials are rice 50g, wheat bran 2.5g, are fitted into culture bottle after siccative is mixed, culture bottle volume is
450mL。
4. the Antrodia camphorata cultural method that a kind of cultured products according to claim 3 and matrix are kept completely separate, feature exist
In: the cinnamomum kanehirai sawdust is the sheet sawdust that cinnamomum kanehirai timber is ground into 0.5cm or so, weighs the Cinnamomum kanahirai hay trees of drying
Bits 0.5-7g is laid in cereal stromal surface.
5. the Antrodia camphorata cultural method that a kind of cultured products according to claim 4 and matrix are kept completely separate, feature exist
In: in the solution containing glucose and inorganic salts, glucose 5g, KH2PO4For 0.5g, MgSO4·7H2O is 0.5g.
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