CN109593758B - Multiplex primer set and method for constructing human B cell immune repertoire based on high-throughput sequencing by using same - Google Patents
Multiplex primer set and method for constructing human B cell immune repertoire based on high-throughput sequencing by using same Download PDFInfo
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- CN109593758B CN109593758B CN201811597255.5A CN201811597255A CN109593758B CN 109593758 B CN109593758 B CN 109593758B CN 201811597255 A CN201811597255 A CN 201811597255A CN 109593758 B CN109593758 B CN 109593758B
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- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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CN111662970B (en) * | 2020-06-29 | 2023-05-05 | 武汉菲沙基因信息有限公司 | Three-generation library-building sequencing method for full-length amplification of BCR immune repertoire |
CN112301099A (en) * | 2020-11-30 | 2021-02-02 | 南方科技大学 | Primer group for amplifying B lymphocyte immune repertoire and application thereof |
CN113215229A (en) * | 2021-04-09 | 2021-08-06 | 深圳荻硕贝肯精准医学有限公司 | Primer group and method for detecting TCRB immune group library of human DNA by high-throughput sequencing |
CN113999891B (en) * | 2021-09-17 | 2024-01-16 | 广东省人民医院 | Methods, a set of primers and a kit for constructing an immune repertoire high throughput sequencing library that removes chimeric sequences in a sample |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102272327A (en) * | 2008-11-07 | 2011-12-07 | 赛昆塔公司 | Methods of monitoring conditions by sequence analysis |
CN103710454A (en) * | 2013-12-31 | 2014-04-09 | 南方科技大学 | Method for TCR or BCR high-throughput sequencing and method for correcting multiple PCR primer deviation by using tag sequence |
CN104560978A (en) * | 2015-01-20 | 2015-04-29 | 中国人民解放军第三军医大学 | Multiplex-polymerase chain reaction (PCR) primer and method for constructing human B cell receptor (BCR) heavy-chain library based on high-throughput sequencing |
CN107779495A (en) * | 2017-10-31 | 2018-03-09 | 中国医学科学院肿瘤医院 | The construction method and kit of T cell antigen receptor diversity sequencing library |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102272327A (en) * | 2008-11-07 | 2011-12-07 | 赛昆塔公司 | Methods of monitoring conditions by sequence analysis |
CN103710454A (en) * | 2013-12-31 | 2014-04-09 | 南方科技大学 | Method for TCR or BCR high-throughput sequencing and method for correcting multiple PCR primer deviation by using tag sequence |
CN104560978A (en) * | 2015-01-20 | 2015-04-29 | 中国人民解放军第三军医大学 | Multiplex-polymerase chain reaction (PCR) primer and method for constructing human B cell receptor (BCR) heavy-chain library based on high-throughput sequencing |
CN107779495A (en) * | 2017-10-31 | 2018-03-09 | 中国医学科学院肿瘤医院 | The construction method and kit of T cell antigen receptor diversity sequencing library |
Non-Patent Citations (2)
Title |
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BCR组库分析乙肝疫苗接种反应及初步机理分析;姜琼 等;《免疫学杂志》;20170930;第33卷(第9期);743-748 * |
Ligation-anchored PCR unveils immune repertoire of TCR-beta from whole blood;Fan Gao and Kai Wang;《BMC Biotechnology》;20150528;1-10 * |
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Denomination of invention: Multiple primer set and method for constructing human B-cell immune group Library Based on high-throughput sequencing using the primer set Effective date of registration: 20220628 Granted publication date: 20211126 Pledgee: Jinan Chengxi sub branch of Qilu Bank Co.,Ltd. Pledgor: SHANDONG ACV BIOTECH CO.,LTD. Registration number: Y2022980009216 |
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Denomination of invention: Multiple primer sets and a method for constructing a human B-cell immune library based on high-throughput sequencing using this primer set Effective date of registration: 20230627 Granted publication date: 20211126 Pledgee: Jinan Chengxi sub branch of Qilu Bank Co.,Ltd. Pledgor: SHANDONG ACV BIOTECH CO.,LTD. Registration number: Y2023980046289 |
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Granted publication date: 20211126 Pledgee: Jinan Chengxi sub branch of Qilu Bank Co.,Ltd. Pledgor: SHANDONG ACV BIOTECH CO.,LTD. Registration number: Y2023980046289 |