CN109588597A - Compound embedded object of a kind of ampelopsis grossdentata element and its preparation method and application - Google Patents
Compound embedded object of a kind of ampelopsis grossdentata element and its preparation method and application Download PDFInfo
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- CN109588597A CN109588597A CN201811624328.5A CN201811624328A CN109588597A CN 109588597 A CN109588597 A CN 109588597A CN 201811624328 A CN201811624328 A CN 201811624328A CN 109588597 A CN109588597 A CN 109588597A
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- solution
- amylose
- mung bean
- preparation
- ampelopsis grossdentata
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- 235000009388 Parthenocissus quinquefolia Nutrition 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 150000001875 compounds Chemical class 0.000 title claims abstract description 22
- 241000219099 Parthenocissus quinquefolia Species 0.000 title claims abstract 9
- 229920001661 Chitosan Polymers 0.000 claims abstract description 68
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims abstract description 58
- 229920000856 Amylose Polymers 0.000 claims abstract description 53
- 240000004922 Vigna radiata Species 0.000 claims abstract description 48
- 235000010721 Vigna radiata var radiata Nutrition 0.000 claims abstract description 48
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 claims abstract description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 230000004048 modification Effects 0.000 claims abstract description 12
- 238000012986 modification Methods 0.000 claims abstract description 12
- 229930014626 natural product Natural products 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 65
- 239000001110 calcium chloride Substances 0.000 claims description 13
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 10
- 238000000502 dialysis Methods 0.000 claims description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 150000004676 glycans Chemical class 0.000 claims description 8
- 238000013019 agitation Methods 0.000 claims description 6
- 229960000583 acetic acid Drugs 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 239000012362 glacial acetic acid Substances 0.000 claims description 5
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 5
- LRWZZZWJMFNZIK-UHFFFAOYSA-N 2-chloro-3-methyloxirane Chemical compound CC1OC1Cl LRWZZZWJMFNZIK-UHFFFAOYSA-N 0.000 claims description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 229960001760 dimethyl sulfoxide Drugs 0.000 claims description 4
- 235000020510 functional beverage Nutrition 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 239000011541 reaction mixture Substances 0.000 claims description 4
- 239000013049 sediment Substances 0.000 claims description 4
- 239000003963 antioxidant agent Substances 0.000 claims description 3
- 230000003078 antioxidant effect Effects 0.000 claims description 3
- 235000006708 antioxidants Nutrition 0.000 claims description 3
- 230000003712 anti-aging effect Effects 0.000 claims description 2
- 235000013361 beverage Nutrition 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 239000002131 composite material Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 239000011149 active material Substances 0.000 abstract description 2
- 239000000969 carrier Substances 0.000 abstract description 2
- 241000563984 Ampelopsis Species 0.000 description 33
- 239000002105 nanoparticle Substances 0.000 description 17
- 239000000463 material Substances 0.000 description 13
- 239000002245 particle Substances 0.000 description 12
- 239000003094 microcapsule Substances 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- 229930003935 flavonoid Natural products 0.000 description 5
- 235000017173 flavonoids Nutrition 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 241000270295 Serpentes Species 0.000 description 4
- 241000219095 Vitis Species 0.000 description 4
- 235000009754 Vitis X bourquina Nutrition 0.000 description 4
- 235000012333 Vitis X labruscana Nutrition 0.000 description 4
- 235000014787 Vitis vinifera Nutrition 0.000 description 4
- -1 flavone compound Chemical class 0.000 description 4
- 230000002209 hydrophobic effect Effects 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000007908 nanoemulsion Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- KJXSIXMJHKAJOD-LSDHHAIUSA-N (+)-dihydromyricetin Chemical compound C1([C@@H]2[C@H](C(C3=C(O)C=C(O)C=C3O2)=O)O)=CC(O)=C(O)C(O)=C1 KJXSIXMJHKAJOD-LSDHHAIUSA-N 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 239000011162 core material Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- KQILIWXGGKGKNX-UHFFFAOYSA-N dihydromyricetin Natural products OC1C(=C(Oc2cc(O)cc(O)c12)c3cc(O)c(O)c(O)c3)O KQILIWXGGKGKNX-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000010148 water-pollination Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/015—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5036—Polysaccharides, e.g. gums, alginate; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Epidemiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Cosmetics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to natural products to develop and use field, in particular to compound embedded object of a kind of ampelopsis grossdentata element and its preparation method and application.The preparation method is the following steps are included: prepare modified mung bean amylose；Modified mung bean amylose/carboxymethyl chitosan nano-solution；Ion water making is spent for ampelopsis grossdentata element solution, modification mung bean amylose/carboxymethyl chitosan nano-solution of equal quality is added, is stirred, pH value is adjusted to neutrality, obtains the compound embedded object of ampelopsis grossdentata element.Mung bean amylose/chitosan composite load system shows good performance in terms of active ingredient carriers, also significant for the body absorption utilization rate for improving active material other than having good slow release effect.
Description
Technical field
Develop and use field the invention belongs to natural products, in particular to a kind of compound embedded object of ampelopsis grossdentata element and its
Preparation method and application.
Background technique
Ampelopsis grossdentata element, also known as dihydromyricetin, extract from a kind of bejuco of Vitaceae Ampelopsis more,
It is a kind of activity flavone compound abundant, has and remove the function such as free radical, anti-oxidant, antithrombotic, antitumor, anti-inflammatory
Effect.But the water solubility of ampelopsis grossdentata element is lower, therefore limits the development and utilization to it.
Most structure is more easily damaged natural flavonoid compound under general condition, unstable.Then it is desirable to
The stabilization of natural flavonoid compound physico-chemical property is improved by approach such as structural modification, microcapsules technologies, improves its dissolubility
Its release etc. can, be controlled in order to apply it in industrial products.
Microcapsules technology is nowadays to quickly grow and one of widely used new and high technology.Microcapsules technology refers to gas
Body, liquid or solid be embedded in it is small and seal capsule in, discharge it can just with speed control under given conditions
Technology.Microcapsules are a spherulas, around it are uniformly wrapped up by material.The substance of the inside is embedded in core material,
And the material for embedding core material formation microcapsules is known as wall material.The size and shape of microcapsules is related with raw material and preparation method.
Common wall material includes lipid, protein, carbon water chemistry object etc., as amylose, mung bean amylose, chitosan, gelatin, Ah
Draw primary glue, maltodextrin, ethyl cellulose etc..Natural flavonoid compound can be kept away to a certain extent after wall material embeds
Exempt from directly affecting for photo-thermal, and have certain slow releasing function, is the effective means for improving natural flavonoid material stability.It is micro-
Capsule technique has important role to the stability for improving natural flavone substance, and the Technological Economy is feasible, but prepares
Particle size be generally several microns to several millimeters and differ, various shapes are unfavorable for body absorption, and mostly use spray drying
Method carry out, preparation process temperature is higher, will cause the loss of natural flavonoid substance.The development of nanotechnology, is filled up
The defect of microcapsules technology.Compared with microcapsules technology, the reaction of nanometer embedding techniques preparation process is mild, particle diameter it is small and
Controllably, it is easier to absorption of human body.Amylose is a kind of wall material for being usually used in embedding fat-soluble food product material, it is by glucose
It is in right-handed helix structure by straight chain molecule made of α-Isosorbide-5-Nitrae glucosides key connection, threaded interior is oleophylic containing only hydrogen atom,
Hydroxyl is located at outside spiral.The special construction of this " inner cavity is hydrophobic, exocoel is hydrophilic ", so that amylose can be used as a kind of master
Body molecule forms inclusion compound by hydrophobic interaction and object flavor molecules, avoids the destruction of light, heat, oxygen etc., can also
Reduce the loss of flavor substance.This project embeds Xian Chi snake Portugal by preparing mung bean amylose/carboxymethyl chitosan nano-emulsion
Grape element, can be greatly improved its stability, expand its application range, improve the shelf life of its industrial products.
Although amylose is as a kind of very common embedding wall material, its water solubility is not also good enough, so that
Its application in industrial-scale production is limited to.It is a kind of although chitosan can encapsulate hydrophilic active
Excellent carrier, but it can only be dissolved in acid solution, insoluble in neutral and alkaline solution, this make the application of chitosan by
Very big limitation is arrived.
Summary of the invention
In order to overcome shortcoming and deficiency existing in the prior art, the primary purpose of the present invention is that providing a kind of aobvious tooth snake
The preparation method of the compound embedded object of grape element.The present invention improves the water soluble characteristic of ampelopsis grossdentata element using the method for embedding, makes
It obtains its industrial application and expands possibility, and use mung bean amylose and carboxymethyl chitosan as the wall material of embedding, it is green
Colour circle is protected；Mung bean amylose is modified by the present invention, is increased its hydrophily, is used carboxymethyl chitosan as composite material
Raw material overcomes the unfavorable factor of acid-soluble chitosan.And mung bean amylose/chitosan is as natural biological compounded material
The characteristics of expecting, being provided simultaneously with amylose and chitosan shows more advantages than amylose or chitosan is used alone.
Another object of the present invention is to provide a kind of ampelopsis grossdentata that above-mentioned preparation method is prepared element compound packet
Bury object.
A further object of the present invention is to provide a kind of applications of the compound embedded object of above-mentioned ampelopsis grossdentata element.
The object of the invention is achieved through the following technical solutions:
A kind of preparation method of the compound embedded object of ampelopsis grossdentata element, comprising the following steps:
(1) the mung bean amylose of 1.5g is dissolved in the anhydrous dimethyl sulphoxide of 5mL and the isopropanol mixed liquor of 5mL,
The NaOH aqueous solution that 12.5mL concentration is 1M is added, is subsequently added into the epoxychloropropane of 2mL；At room temperature, it is stirred in nitrogen
48h；With the pH value of hydrochloric acid adjusting reaction solution to 7.0, with acetone precipitation reaction mixture, gained sediment deionized water is molten
Solution, is transferred in bag filter, is that dialysis medium carries out dialysis purification at room temperature with deionized water；After dialysis, collect saturating
Sample in analysis bag is freeze-dried, the modification mung bean amylose purified；
(2) ion water making is spent for the carboxymethyl chitosan solution of 1-4mg/mL, with the glacial acetic acid tune of mass fraction 0.1%
The pH value of carboxymethyl chitosan solution is saved to neutrality；5mL carboxymethyl chitosan solution is taken, modified mung bean amylose is added, obtains
To mixed solution, wherein the weight ratio of the carboxymethyl shell in carboxymethyl chitosan solution and modified mung bean amylose is 1:1-4；
Under 800rpm magnetic agitation, into mixed solution plus 1-3mL concentration is the CaCl of 1-5mg/mL2Solution continues to stir 30-
Modified mung bean amylose/carboxymethyl chitosan nano-solution is made in 120min；
(3) ion water making is spent for ampelopsis grossdentata element solution, and modification mung bean amylose/carboxylic first of equal quality is added
Base enclosure glycan nano-solution, stirring adjust pH value to neutrality, obtain the compound embedded object of ampelopsis grossdentata element.
The molecular cut off of step (1) described bag filter is 1000Da.
The concentration of step (2) described carboxymethyl chitosan solution is 1mg/mL, CaCl2The concentration of solution is 1mg/mL,
CaCl2The dosage of solution is 2mL.
The weight ratio of carboxymethyl shell and modified mung bean amylose in step (2) described carboxymethyl chitosan solution is 1:
1。
The time of step (2) described stirring is 30min.
A kind of compound embedded object of ampelopsis grossdentata element being prepared by above-mentioned preparation method.
The above-mentioned compound embedded object of ampelopsis grossdentata element is in preparing functional beverage or in natural products drug field
Using.
The functional beverage is the beverage with anti-oxidant, anti-aging, anti-fatigue effect.
The concentration of carboxymethyl chitosan solution is preferably 1mg/mL in step (2), because with carboxymethyl chitosan concentration
Increase, the partial size of nanoparticle has the trend significantly increased, the raising of the concentration of carboxymethyl chitosan, the viscosity meeting of mixed liquor
Increase, biggish viscosity will lead to the increase of solution surface tension, its diffusion process is prevented quickly to carry out, therefore what be will form receives
Rice grain can mutually be put attached, form bigger micelle particle；
Carboxymethyl shell and the weight ratio of modified mung bean amylose in step (2) in carboxymethyl chitosan solution are preferably
1:1, because the mung bean amylose of low concentration can promote itself to be combined into carboxymethyl chitosan, to form greater particle size
Particle, and high concentration mung bean amylose, because it generates the intermolecular interactions such as more hydrogen bonds between carboxymethyl chitosan
Power, to be formed compared with small particle nano particle.When the mass ratio of mung bean amylose and carboxymethyl chitosan is 1:1, than not having
There is the partial size of the nano particle of addition mung bean amylose big, but its PDI (polydispersity index) becomes smaller, therefore the nanometer formed
The distribution of particle and dispersion degree are higher, comprehensively consider, and select 1:1 optimal；
CaCl in step (2)2The concentration of solution is preferably 1mg/mL because with CaCl2The increase of concentration, partial size and PDI
All it is gradually increased.This is because a large amount of CMC strand and CaCl2Crosslinked together, more intermolecular hydrogen bondings are formed, so that
Partial size increases significantly, and nano particle is easily clumped together, and dispersibility reduces；
CaCl in step (2)2The dosage of solution is preferably 2mL, because working as carboxymethyl chitosan and CaCl2Mass ratio be
When 5:1, nanometer particle size is larger, it may be possible to because of CaCl2Quality it is lower, fail sufficiently with the-COO- of carboxymethyl chitosan make
With, therefore the particle formed is loose, and dispersibility is poor；When carboxymethyl chitosan and CaCl2Mass ratio be 5:2 when, carboxylic first
Base enclosure glycan and CaCl2Crosslinking ratio is appropriate, so that compact state is presented in nano particle, partial size is smaller.；Work as carboxymethyl chitosan
With CaCl2Mass ratio be 5:3 when, the partial size of nano particle increases accordingly, this is because carboxymethyl chitosan quality increase after,
The Contact-sorption chance that nano particle occurs increases, and is easy so that particle is assembled, furthermore, it is possible to be because of carboxymethyl shell
Glycan is continuously added, and the nanoparticle group of the nano particle formed or reunion before having coated leads to solution system
Particle size dispersion is uneven, thus PDI is also increase accordingly；
Selection mixing time is preferably 30min in step (2), because with the increase of mixing time, the grain of nano particle
The diameter change very big there is no generation, but the trend increased is presented in its PDI, this is because under certain speed of agitator, it is molten
Nano particle under the action of shear force, is uniformly dispersed in liquid, fully reacting when 30min, and it is uniform to form size
Particle.When extending to 120min between upon agitation, in view of the increase of mixing time, in the case where shearing force constantly acts on, solute
Molecular motion can enhance, and droplet collision chance increases, and mechanical stirring overlong time can cause breakage, factors above to nano particle
It is likely to lead to the generation of nano particle fragment, and increases PDI.
Compared with prior art, the present invention has the following advantages and beneficial effects:
(1) mung bean amylose is a kind of very common embedding wall material, because it possesses surface hydrophilic, internal cavity is hydrophobic
Special construction, be very suitable to embed some natural unstable hydrophobic substances, such as ampelopsis grossdentata element, make after embedding
The product of ampelopsis grossdentata element can greatly improve industrial condition adaptability.
(2) chitosan is a kind of environmentally friendly compound, has biological degradability and biocompatibility, is widely used
In the preparation of nano particle.
(3) mung bean amylose/chitosan of the present invention has been provided simultaneously with mung bean straight chain shallow lake as natural biological compounded material
The characteristics of powder and chitosan, shows more advantages than chitosan or mung bean amylose is used alone.Mung bean amylose/shell
Glycan composite load system shows good performance in terms of active ingredient carriers, right other than having good slow release effect
It is also significant in the body absorption utilization rate for improving active material.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Embodiment 1
(1) preparation of modified mung bean amylose
The mung bean amylose of 1.5g is dissolved in the anhydrous dimethyl sulphoxide of 5mL and the isopropanol mixed liquor of 5mL, is added
The NaOH aqueous solution of the 1M of 12.5mL, is subsequently added into the epoxychloropropane of 2mL.At room temperature, 48h is stirred in nitrogen.Use hydrochloric acid
The pH value of reaction solution is adjusted to 7.0, with excessive acetone precipitation reaction mixture, a small amount of deionized water of gained sediment
Dissolution, is transferred in bag filter (MWCO, 1000Da), is that dialysis medium carries out dialysis purification at room temperature with deionized water.Thoroughly
After analysis, the sample collected in bag filter is freeze-dried, the modification mung bean amylose purified.
(2) preparation of modified mung bean amylose carboxymethyl chitosan nano particle
Ion water making is spent for the carboxymethyl chitosan solution of 1mg/mL, adjusts carboxylic with the glacial acetic acid of mass concentration 0.1%
The pH value of methyl chitosan solution takes 5mL carboxymethyl chitosan solution, is added and carboxylic first in carboxymethyl chitosan solution to neutrality
The weight ratio of base enclosure glycan is the modification mung bean amylose of 1:1, and under 800rpm magnetic agitation, 2mL is added dropwise to the mixed liquor of 5mL
CaCl2Solution (1mg/mL) continues to stir 30min, and modified mung bean amylose carboxymethyl chitosan nano-solution is made.
(3) ion water making is spent for ampelopsis grossdentata element solution, and the modification mung bean amylose carboxylic first of equal quality is added
Base enclosure glycan nano-solution, stirring adjust pH value to neutrality, obtain the compound embedded object of ampelopsis grossdentata element.
Embodiment 2
(1) preparation of modified mung bean amylose
The mung bean amylose of 1.5g is dissolved in the anhydrous dimethyl sulphoxide of 5mL and the isopropanol mixed liquor of 5mL, is added
The NaOH aqueous solution of the 1M of 12.5mL, is subsequently added into the epoxychloropropane of 2mL.At room temperature, 48h is stirred in nitrogen.Use hydrochloric acid
The pH value of reaction solution is adjusted to 7.0, with excessive acetone precipitation reaction mixture, a small amount of deionized water of gained sediment
Dissolution, is transferred in bag filter (MWCO, 1000Da), is that dialysis medium carries out dialysis purification at room temperature with deionized water.Thoroughly
After analysis, the sample collected in bag filter is freeze-dried, the modification mung bean amylose purified.
(2) preparation of modified mung bean amylose carboxymethyl chitosan nano-solution
Ion water making is spent for the carboxymethyl chitosan solution of 1mg/mL, adjusts carboxylic with the glacial acetic acid of mass fraction 0.1%
The pH value of methyl chitosan solution takes 5mL carboxymethyl chitosan solution, is added and carboxylic first in carboxymethyl chitosan solution to neutrality
The weight ratio of base enclosure glycan is the modification mung bean amylose of 1:2, and under 800rpm magnetic agitation, 2mL is added dropwise to the mixed liquor of 5mL
CaCl2Solution (1mg/mL) continues to stir 60min, and modified mung bean amylose carboxymethyl chitosan nano-solution is made.
(3) ion water making is spent for ampelopsis grossdentata element solution, and the modified mung bean amylose carboxymethyl of equal quality is added
Chitosan nano solution, stirring adjust pH value to neutrality, obtain the compound embedded object of ampelopsis grossdentata element.
Comparative example 1
(1) preparation of carboxymethyl chitosan nano-solution
Ion water making is spent for the carboxymethyl chitosan solution of 1mg/mL, adjusts carboxylic with the glacial acetic acid of mass concentration 0.1%
The pH of methyl chitosan solution takes 5mL carboxymethyl chitosan solution to neutrality, under 800rpm magnetic agitation, to the mixed liquor of 5mL
2mL CaCl is added dropwise2Solution (1mg/mL) continues to stir 30min, carboxymethyl chitosan nano-solution is made.
(2) preparation of ampelopsis grossdentata element load carboxymethyl chitosan nano particle
Ion water making is spent for ampelopsis grossdentata element solution, carboxymethyl chitosan obtained by equal quality step (1) is added and receives
Rice solution, stirring adjust pH value to neutrality, obtain ampelopsis grossdentata element load carboxymethyl chitosan nano-solution.
Carboxylic is loaded to the compound embedded object of the element of ampelopsis grossdentata obtained by Examples 1 and 2 and 1 gained ampelopsis grossdentata element of comparative example
Methyl chitosan nano-solution carries out compliance test result:
Respectively take 1 gained ampelopsis grossdentata element of the compound embedded object of the element of ampelopsis grossdentata obtained by 7mL Examples 1 and 2 and comparative example
It loads carboxymethyl chitosan nano-solution high-speed low temperature and is centrifuged (120000rpm；30min；4 DEG C), supernatant fluid is taken, is transferred to
0.5mL formic acid, constant volume is added in 10mL volumetric flask.The content that ampelopsis grossdentata element in supernatant is measured with HPLC, according to public once
The encapsulation rate and load factor of formula calculating ampelopsis grossdentata nanometer.
In formula: m1 is ampelopsis grossdentata element input amount, and m2 is the amount of ampelopsis grossdentata element in supernatant, and m is Xian Chi snake Portugal
The weight of grape element nano particle.
It was found that using modified mung bean amylose/carboxymethyl chitosan composite nano-emulsion than carboxymethyl chitosan is used alone
Nano-emulsion can improve 10% or so for the encapsulation rate and load factor of ampelopsis grossdentata element.And it can be with Xian Chi snake Portugal
Grape element generates clathration, to obstruct loss of the photo-thermal to ampelopsis grossdentata element to a certain extent, improves ampelopsis grossdentata
The photo and thermal stability of element.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (8)
1. a kind of preparation method of the compound embedded object of ampelopsis grossdentata element, it is characterised in that the following steps are included:
(1) the mung bean amylose of 1.5g is dissolved in the anhydrous dimethyl sulphoxide of 5mL and the isopropanol mixed liquor of 5mL, is added
12.5mL concentration is the NaOH aqueous solution of 1M, is subsequently added into the epoxychloropropane of 2mL；At room temperature, 48h is stirred in nitrogen；With
Hydrochloric acid adjusts the pH value of reaction solution to 7.0, and with acetone precipitation reaction mixture, gained sediment deionized water dissolving turns
It moves in bag filter, is that dialysis medium carries out dialysis purification at room temperature with deionized water；After dialysis, collect in bag filter
Sample be freeze-dried, the modification mung bean amylose purified；
(2) ion water making is spent for the carboxymethyl chitosan solution of 1-4mg/mL, adjusts carboxylic with the glacial acetic acid of mass fraction 0.1%
The pH value of methyl chitosan solution is to neutrality；5mL carboxymethyl chitosan solution is taken, modified mung bean amylose is added, is mixed
Solution is closed, wherein the weight ratio of the carboxymethyl shell in carboxymethyl chitosan solution and modified mung bean amylose is 1:1-4；?
Under 800rpm magnetic agitation, into mixed solution plus 1-3mL concentration is the CaCl of 1-5mg/mL2Solution continues to stir 30-
Modified mung bean amylose/carboxymethyl chitosan nano-solution is made in 120min；
(3) ion water making is spent for ampelopsis grossdentata element solution, and modification mung bean amylose/carboxymethyl shell of equal quality is added
Glycan nano-solution, stirring adjust pH value to neutrality, obtain the compound embedded object of ampelopsis grossdentata element.
2. preparation method according to claim 1, it is characterised in that: the molecular cut off of step (1) described bag filter is
1000Da。
3. preparation method according to claim 1, it is characterised in that: step (2) described carboxymethyl chitosan solution it is dense
Degree is 1mg/mL, CaCl2The concentration of solution is 1mg/mL, CaCl2The dosage of solution is 2mL.
4. preparation method according to claim 1, it is characterised in that: in step (2) described carboxymethyl chitosan solution
The weight ratio of carboxymethyl shell and modified mung bean amylose is 1:1.
5. preparation method according to claim 1, it is characterised in that: the time of step (2) described stirring is 30min.
6. a kind of compound embedded object of ampelopsis grossdentata element being prepared by preparation method described in claim 1.
7. the compound embedded object of ampelopsis grossdentata according to claim 6 element is in preparing functional beverage or natural products medicine
Application in object field.
8. application according to claim 7, it is characterised in that: the functional beverage is with anti-oxidant, anti-aging, resists
The beverage of fatigue function.
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| CN110101871A (en) * | 2019-05-30 | 2019-08-09 | 广东工业大学 | A kind of preparation method embedding resveratrol |
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