CN109588210A - Artificial induction's method of stone-like omphalia fructification - Google Patents
Artificial induction's method of stone-like omphalia fructification Download PDFInfo
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- CN109588210A CN109588210A CN201910012112.1A CN201910012112A CN109588210A CN 109588210 A CN109588210 A CN 109588210A CN 201910012112 A CN201910012112 A CN 201910012112A CN 109588210 A CN109588210 A CN 109588210A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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Abstract
The invention belongs to Chinese medicine field of planting, and in particular to a kind of artificial induction's method of stone-like omphalia fructification.Stone-like omphalia fructification artificial induction's method of the invention specifically includes original seed preparation, cultivar preparation, elicitor entity and labor management method.The time that stone-like omphalia generates fructification can be extended to 3 months or more using method of the invention, break that the stone-like omphalia fructification service life is short, is difficult to the status obtained, stone-like omphalia fructification is induced using this method, material base and technical support can be provided for the development and application of stone-like omphalia biological status and studies of the life histories, fine-variety breeding and fructification.
Description
Technical field
The invention belongs to Chinese medicine field of planting, and in particular to a kind of artificial induction's method of stone-like omphalia fructification.
Background technique
Stone-like omphalia is a kind of medicinal fungi, also known as bamboo Siberian cocklebur, Lei Shi, thunder arrow etc., is listed as " three Siberian cockleburs with Poria cocos, umbellate pore furgus.
Fructification is the sexual propagation organ of macro fungi, can produce a large amount of basidiospore.It is excellent by cultivating basidiospore acquisition
Non-defective unit kind is medicine, the common technological means of edible mushroom fine-variety breeding.Stone-like omphalia is as a kind of large-scale medicinal fungi, the breeding of kind
It needs to generate basidiospore by artificial induction's fructification, and then obtains strain excellent.
At present in the pharmaceutical texts and related article in relation to stone-like omphalia, dispute is remained to its classification position, some scholars recognize
Belong to Polyporaceae for stone-like omphalia, some are then considered Tricholomataceae.Fructification is one kind that macro fungi generates basidia and basidiospore
Highly organized structure, the difference of growth course, form, microstructure etc. are of great significance on taxology, still
Since the service life of its fructification is very short, wild fructification is difficult to obtain, and cultivating fructification is the key that its determining classification position.
In addition, the stone-like omphalia cellulose content in fructification, which has reached 5.93%, to be found to the quality analysis of fructification by the author,
Considerably beyond the active constituent content 0.6% of States Pharmacopoeia specifications；It is tested and is found by external nematicidal, stone-like omphalia fructification powder
Aqueous solution has significant killing effect to nematode, can be used as the natural substitute of insecticidal materials.
In consideration of it, being research, fine-variety breeding and the fructification of stone-like omphalia biological status it is necessary to artificial induction's fructification
Development and application provides technical support and material base.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of artificial induction's method of stone-like omphalia fructification, this method is by thunder
The time that ball generates fructification extends to 3 months or more, has broken that the stone-like omphalia fructification service life is short, is difficult to the status obtained, and material
Material is easy to get.
To achieve the above object, the technical solution of the present invention is as follows:
Artificial induction's method of stone-like omphalia fructification, which comprises the following steps:
(1) prepared by original seed
From in picking stone-like omphalia strain access original seeds bottle, original seeds bottle is expired in culture to Omphalia lapidescens filament length in parent species test tube；
(2) prepared by cultivation strain
A. prepared by compost: taking toothed oak sawdust, one or more, the acquisition compost base of corn flour, wheat bran, dregs of beans is added
Matter, carbon-nitrogen ratio are 290~360；Based on the weight percent of compost matrix, gypsum, the 0.1- of 0.5-2% is added
0.3% KH2PO4With the MgSO of 0.05-0.2%4, water is added after mixing, makes water content 50-70%, mixes thoroughly, is obtained
Compost；
B. compost sterilizes after being packed into seed bottle；
C. it is inoculated with and cultivates: stone-like omphalia strain being taken to be transferred in seed bottle from the original seeds bottle of step (1), culture to stone-like omphalia mycelia
Cover with bottle；
(3) fructification is induced
The cultivar that step (2) obtains keeps mushroom room or greenhouse temperature after sand cultivation is covered in mushroom room or greenhouse or heap is built
16-32 DEG C, relative air humidity 80-95%, inducing fructification illumination condition early period is 1000-2500lx, sporophore growth
Required illumination condition is 50-2500lx.
Preferably, in the culture material matrix, based on toothed oak sawdust weight percent, comprising 0.1~5.7% corn flour, 2
~35.6% wheat bran, 0.1~3.2% dregs of beans.
Further, in the culture material matrix, based on toothed oak sawdust weight percent, comprising 5.7~25.9% wheat bran,
2.1~5.7% corn flour, 0.7~2.1% dregs of beans.
Preferably, step (3) is described covers sand cultivation specifically: to liming powder on mushroom room or greenhouse ground to sterilize,
Sanding soil 5-6cm is thick, and cultured cultivation strain is kept intact taking-up, vertical with the gap 2~5cm wide between strain and strain
It puts, upward with bottleneck end, then with sand fills up gap, cover strain.
Further, the height of sand covering strain should just cover strain top, not more than strain top out
0.5cm。
Preferably, step (3) heap is built specifically: to powder is limed on mushroom room or greenhouse ground to sterilize, is opened and is planted
Bottle cap is cultivated, heap builds 5~6 layers of cultivar.
Preferably, water quality score is 55-65% in the compost.
Preferably, the coarse granule that the soil of mushroom room or greenhouse is 40~60% using aqueous mass percent in step (3)
River sand.
Preferably, step (3) the mushroom room or greenhouse temperature are 25~30 DEG C.
Preferably, step (1) original seed or step (2) the cultivar condition of culture are protected from light under the conditions of being 22~25 DEG C
Culture.
Preferably, step (2) sterilizing specifically: under 103-115kPa under 121~126 DEG C of sterilizing 1.5h or normal pressure
100~105 DEG C of 10~12h of sterilizing.
Preferably, step (1) original seed preparation detailed process includes:
A. prepared by original seed matrix: by niblet boiling, filtering is added 1~3% based on the mass percent of niblet
Gypsum, 0.05~0.2% MgSO4With 0.1~0.3% KH2PO4, it stirs and evenly mixs, is fitted into original seeds bottle in 121~126 DEG C,
103~115kPa sterilizing 1.5h, cooling；
B. it is inoculated with: being accessed in original seeds bottle from picking stone-like omphalia strain in parent species test tube；
C. cultivate: the original seeds bottle after inoculation is protected from light to culture to Omphalia lapidescens filament length under the conditions of 22~25 DEG C expires original seeds bottle.
The beneficial effects of the present invention are: the method for stone-like omphalia fructification artificial induction provided by the invention:
1) for the present invention using toothed oak sawdust as major ingredient, dregs of beans, wheat bran, corn flour are auxiliary material, guarantee the carbon-nitrogen ratio (C/N) of cultivar
Between 290~360, the induction for being conducive to fructification is generated；Method of the invention can prolong the time that stone-like omphalia generates fructification
Length solved the problems, such as that the stone-like omphalia fructification service life was short, is difficult to obtain to 3 months or more；
2) stone-like omphalia fructification is induced using this method, can is stone-like omphalia biological status and studies of the life histories, breeding choosing
It educates and the development and application of fructification in future provides material base；
3) planting material that uses is cheap, is easy to get, and operating method is also relatively simple, the stone-like omphalia fructification cost of acquisition compared with
It is low.
Detailed description of the invention
The culture figure of Fig. 1 cultivar；
Stone-like omphalia fructification soil fruiting operating method figure in Fig. 2 embodiment 1；
The stone-like omphalia fructification induced in Fig. 3 embodiment 1；
The stone-like omphalia fructification induced in Fig. 4 embodiment 2；
The stone-like omphalia fructification induced in Fig. 5 embodiment 3；
The stone-like omphalia fructification induced in Fig. 6 embodiment 3；
The stone-like omphalia fructification induced in Fig. 7 embodiment 3；
The tender stone-like omphalia fructification of Fig. 8.
Specific embodiment
The preferred embodiment of the present invention will be described in detail (referring to attached drawing) below.Tool is not specified in preferred embodiment
The experimental method of concrete conditions in the establishment of a specific crime, usually according to normal condition, illustrated embodiment are to preferably say to the contents of the present invention
It is bright, but be not that the contents of the present invention are only limitted to illustrated embodiment.So those skilled in the art are according to foregoing invention
Content carries out nonessential modifications and adaptations to embodiment, still falls within protection scope of the present invention.
Embodiment 1
(1) prepared by original seed
A. prepared by original seed matrix
Selection adds boiling to endosperm to deposit the white heart of a line without the niblet for free from insect pests of going rotten, and filters, by the quality hundred of niblet
Divide than meter, 2% gypsum, 0.1% MgSO is added4, 0.2% KH2PO4, stir and evenly mix, be fitted into 650mL original seeds bottle, 121
DEG C, sterilize under 103-115kPa 1.5h, be cooled to room temperature to obtain the final product.
B. it is inoculated with
Inoculation need to operate on clean bench.Before inoculation, transfer room should carry out ultraviolet lamp sterilizing, first with 75% alcohol to both hands
And transfer needle disinfection, transfer needle, test tube mouth and tampon all need to be sterilized with alcolhol burner flame.Selection grow vigorous, cell age compared with
The strain that colouring matter secretion object not yet occurs in short, subiculum is inoculated with, and is hooked in parent species test tube with transfer needle and is taken 1cm2Size
Fungus block is transferred in original seeds bottle.
C. it cultivates
Original seeds bottle after inoculation is transferred to the culturing room being protected from light to cultivate under the conditions of 22~25 DEG C of temperature to stone-like omphalia mycelia
After covering with original seeds bottle.
(2) prepared by cultivation strain
A. weighing: weighing major ingredient toothed oak sawdust, and following auxiliary material: 5.7% wheat is then weighed based on toothed oak sawdust weight percent
Bran, 5.7% corn flour, 1.1% dregs of beans, C/N 357；By the percentages of above-mentioned material gross weight, 1% gypsum is added,
0.2% KH2PO4, 0.1% MgSO4。
B. spice: water water content into compost is added after each material is mixed and is mixed thoroughly up to 60%.
C. it packs: the compost after mixing thoroughly being fitted into 750ml seed bottle, side rim pressure, until consolidation, elastic need are uniform,
Then bottle cap is covered.
D. it sterilizes: seed bottle being placed in 103~115kPa, 121~126 DEG C of sterilizing 90min, naturally cools to room after sterilizing
Temperature.
E. it is inoculated with: being inoculated in superclean bench or inoculating hood after sterilizing, with the wiping of 75% ethanol for disinfection before being inoculated with
Hand, rifle tweezer, then rifle tweezer is placed in calcination on alcolhol burner flame, take stone-like omphalia strain 2g to be transferred to cultivation strain in original seeds bottle after cooling
In bottle.
F. it cultivates: the seed bottle after inoculation being transferred to the culturing room being protected from light and is cultivated under the conditions of 22~25 DEG C of temperature to thunder
After ball mycelia is covered with, visible attached drawing 1 is cultivated.
(3) soil fruiting
Earthing operation: to powder is limed on mushroom room floor to sterilize, 5~6cm thickness sand is spread on ground, by cultured cultivation
Strain is kept intact taking-up, and strain, with vertical display at a distance from 5cm (upward with bottleneck end), then is filled up with sand between strain
Gap, sand cover strain with a thickness of 0.5cm, the visible attached drawing 2 of mode of operation.
Daily management: 27 DEG C of mushroom room is kept, soil moisture content 60%, relative air humidity 90%, early period, elicitor was real
Body intensity of illumination is 700lx, and sporophore growth intensity of illumination is 200lx.
As a result describe: cultivar C/N is 357, using toothed oak sawdust as Cultivation use, is aided with 5.7% wheat bran, 5.7%
Corn flour, 1.1% dregs of beans, be suitable as induction fructification formula；Earthing operation and daily management are also adapted for son
The induction and growth of entity.Fructification that this method is induced is healthy and strong, after fructification is mature, cap central dip is funnel-shaped,
3~6cm of diameter, cap is in light brown, lamella white, light brown, the hollow, long 4~8cm in stem upper end white lower end, stem base
Portion slightly thickening, have white fluff, as a result visible attached drawing 3.
Embodiment 2
(1) prepared by original seed
A. prepared by original seed matrix
Selection adds boiling to endosperm to deposit the white heart of a line without the niblet for free from insect pests of going rotten, and filters, by the quality hundred of niblet
Divide than meter, 2% gypsum, 0.1% MgSO is added4, 0.2% KH2PO4, stir and evenly mix, be fitted into 650mL original seeds bottle, 121
DEG C, sterilize under 103-115kPa 90min, be cooled to room temperature to obtain the final product.
B. it is inoculated with
Inoculation need to operate on clean bench.Before inoculation, transfer room should carry out ultraviolet lamp sterilizing, first with 75% alcohol to both hands
And transfer needle disinfection, transfer needle, test tube mouth and tampon all need to be sterilized with alcolhol burner flame.Selection grow vigorous, cell age compared with
The strain that colouring matter secretion object not yet occurs in short, subiculum is inoculated with, and is hooked in parent species test tube with transfer needle and is taken 1cm2Size
Fungus block is transferred in original seeds bottle.
C. it cultivates
Original seeds bottle after inoculation is transferred to the culturing room being protected from light to cultivate under the conditions of 22~25 DEG C of temperature to stone-like omphalia mycelia
After covering with original seeds bottle.
(2) prepared by cultivation strain
A. weighing: weighing major ingredient toothed oak sawdust, and following auxiliary material: 35.6% wheat is then weighed based on toothed oak sawdust weight percent
Bran, 4.27% corn flour, 0.7% dregs of beans, C/N 292；By the percentages of above-mentioned material gross weight, 1% stone is added
Cream, 0.1% MgSO4, 0.2% KH2PO4。
B. spice: it is 60% that water water content into compost is added after each material is mixed, and is mixed thoroughly.
C. it packs: the compost after mixing thoroughly being fitted into 750ml seed bottle, side rim pressure, until consolidation, elastic need are uniform,
Then bottle cap is covered.
D. it sterilizes: seed bottle being placed in 103~115kPa, 121~126 DEG C of sterilizing 90min, naturally cools to room after sterilizing
Temperature.
E. it is inoculated with: being inoculated in superclean bench or inoculating hood after sterilizing, with the wiping of 75% ethanol for disinfection before being inoculated with
Hand, rifle tweezer, then rifle tweezer is placed in calcination on alcolhol burner flame, take stone-like omphalia strain 2g to be transferred to cultivation strain in original seeds bottle after cooling
In bottle.
F. it cultivates: the seed bottle after inoculation being transferred to the culturing room being protected from light and is cultivated under the conditions of 22~25 DEG C of temperature to thunder
After ball mycelia is covered with.
(3) convex furrow face yin canopy fruiting: to powder is limed on greenhouse ground to sterilize, opening strain bottle cap, heap build strain 5~
6 layers, keeping relative air humidity 95%, 25 DEG C of greenhouse temperature, induction fructification intensity of illumination is 2500lx, sporophore growth light
It is 2000lx according to intensity.
As a result describe: cultivar C/N is 292, using toothed oak sawdust as Cultivation use, is aided with 35.6% wheat bran,
4.27% corn flour, 0.7% dregs of beans are suitable as the formula of induction fructification；The mode of convex furrow face yin canopy fruiting is produced
Raw fructification is healthy and strong, and after fructification is mature, cap central dip is in nearly funnel type, 2~4cm of diameter, and cap is in crineous, bacterium
Pleat white, stem upper end white lower end is light brown, hollow, long 4~7cm, stem base portion slightly thickening, have white fluff, as a result may be used
See attached drawing 4.
Embodiment 3
(1) prepared by original seed
A. prepared by original seed matrix
Selection adds boiling to endosperm to deposit the white heart of a line without the niblet for free from insect pests of going rotten, and filters, by the quality hundred of niblet
Divide than meter, 2% gypsum, 0.1% MgSO is added4, 0.2% KH2PO4, stir and evenly mix, be fitted into 650mL original seeds bottle, 121
DEG C, sterilize under 103-115kPa 90min, be cooled to room temperature to obtain the final product.
B. it is inoculated with
Inoculation need to operate on clean bench.Before inoculation, transfer room should carry out ultraviolet lamp sterilizing, first with 75% alcohol to both hands
And transfer needle disinfection, transfer needle, test tube mouth and tampon all need to be sterilized with alcolhol burner flame.Selection grow vigorous, cell age compared with
The strain that colouring matter secretion object not yet occurs in short, subiculum is inoculated with, and is hooked in parent species test tube with transfer needle and is taken 1cm2Size
Fungus block is transferred in original seeds bottle.
C. it cultivates
Original seeds bottle after inoculation is transferred to the culturing room being protected from light to cultivate under the conditions of 22~25 DEG C of temperature to stone-like omphalia mycelia
After covering with original seeds bottle.
(2) prepared by cultivation strain
A. weighing: weighing major ingredient toothed oak sawdust, and following auxiliary material: 19.4% wheat is then weighed based on toothed oak sawdust weight percent
Bran, 5.1% corn flour, 3.2% dregs of beans, C/N 319；By the percentages of above-mentioned material gross weight, 1% gypsum is added,
0.1% MgSO4, 0.2% KH2PO4。
B. spice: water water content into compost is added after each material is mixed and is mixed thoroughly up to 60%.
C. it packs: the compost after mixing thoroughly being fitted into 750ml seed bottle, side rim pressure, until consolidation, elastic need are uniform,
Then bottle cap is covered.
D. it sterilizes: seed bottle being placed in 103~115kPa, 121~126 DEG C of sterilizing 90min, naturally cools to room after sterilizing
Temperature.
E. it is inoculated with: being inoculated in superclean bench or inoculating hood after sterilizing, with the wiping of 75% ethanol for disinfection before being inoculated with
Hand, rifle tweezer, then rifle tweezer is placed in calcination on alcolhol burner flame, take stone-like omphalia strain 2g to be transferred to cultivation strain in original seeds bottle after cooling
In bottle.
F. it cultivates: the seed bottle after inoculation being transferred to the culturing room being protected from light and is cultivated under the conditions of 22~25 DEG C of temperature to thunder
Ball mycelia is covered with.
(3) earthing operates: to powder is limed on mushroom room floor to sterilize, 5~6cm thickness sand is spread on ground, it will be cultured
Cultivar is kept intact taking-up, with 1 finger beam gap vertical display (upward with bottleneck end) between strain and strain, then is filled out with sand
Full gap, sand cover strain with a thickness of 0.5cm.
Daily management: 30 DEG C of mushroom room is kept, soil moisture content 60%, relative air humidity 90%, early period, elicitor was real
Body intensity of illumination is 2000lx, and sporophore growth intensity of illumination is 2000lx.
As a result describe: cultivar C/N is 319, using toothed oak sawdust as Cultivation use, is aided with 19.4% wheat bran, 5.1%
Corn flour, 3.2% dregs of beans, be suitable as induction fructification formula；Fructification caused by mode is healthy and strong under this condition,
After fructification is mature, the be in form of cap difference, most central dip, central peripheral are slightly raised and thicken, cap edge
It slightly turns up, portion centers are recessed, cap is turned over by symmetry axis of center, and portion centers are recessed, the asymmetric part of cap turns over, directly
4~7cm of diameter, the brown edge white in cap center, lamella white, the light brown lower end in stem upper end gradually deep, hollow, long 3~
5cm, stem base portion slightly thickening, by white fluff, as a result visible attached drawing 5, Fig. 6, Fig. 7.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
Claims (10)
1. artificial induction's method of stone-like omphalia fructification, which comprises the following steps:
(1) prepared by original seed
From in picking stone-like omphalia strain access original seeds bottle, original seeds bottle is expired in culture to Omphalia lapidescens filament length in parent species test tube；
(2) prepared by cultivation strain
A. prepared by compost: toothed oak sawdust is taken, one or more, the acquisition compost matrix of corn flour, wheat bran, dregs of beans is added,
Carbon-nitrogen ratio is 290~360；Based on the weight percent of compost matrix, the gypsum of 0.5-2%, 0.1-0.3% is added
KH2PO4With the MgSO of 0.05-0.2%4, water is added after mixing, makes water content 50-70%, mixes thoroughly, obtains compost；
B. compost sterilizes after being packed into seed bottle；
C. it is inoculated with and cultivates: taking stone-like omphalia strain to be transferred in cultivation strain bottle from the original seeds bottle of step (1), culture to stone-like omphalia mycelia
Cover with seed bottle；
(3) fructification is induced
The cultivar that step (2) obtains keeps mushroom room or greenhouse temperature 16-32 after soil covering culture or heap are built in mushroom room or greenhouse
DEG C, relative air humidity 80-95%, inducing fructification illumination condition early period is 1000-2500lx, sporophore growth institute light requirement
It is 50-2500lx according to condition.
2. artificial induction's method according to claim 1, characteristic are, in the culture material matrix, by toothed oak sawdust weight
Measure percentages, comprising 0.1~5.7% corn flour, 2~35.6% wheat bran, 0.1~3.2% dregs of beans.
3. artificial induction's method according to claim 2, characteristic are, in the culture material matrix, by toothed oak sawdust weight
Measure percentages, comprising 2.1~5.7% corn flour, 5.7~25.9% wheat bran, 0.7~2.1% dregs of beans.
4. artificial induction's method according to claim 1, characteristic are, step (3) is described to cover sand cultivation specifically:
To powder is limed on mushroom room or greenhouse ground to sterilize, sanding soil 5-6cm is thick, and cultured cultivar is kept intact taking-up,
With the gap 2~5cm wide vertical display between strain and strain, upward with bottleneck end, then with sand fills up gap, cover strain.
5. artificial induction's method according to claim 4, characteristic are, the height of the sand covering strain should be just
Strain top is covered, without departing from strain top 0.5cm.
6. artificial induction's method according to claim 1, characteristic are, step (3) heap is built specifically: to mushroom room
Or the powder that limes on greenhouse ground opens cultivar bottle cap to sterilize, heap builds 5~6 layers of cultivar.
7. artificial induction's method according to claim 1, characteristic are, water quality score is 55- in the compost
65%.
8. artificial induction's method according to claim 1, characteristic are, the soil of mushroom room or greenhouse makes in step (3)
The coarse granule river sand for being 40~60% with aqueous mass percent.
9. artificial induction's method according to claim 1, characteristic are, step (3) the mushroom room or greenhouse temperature are
25~30 DEG C.
10. artificial induction's method according to claim 1, which is characterized in that step (1) original seed or step (2) institute
The condition of culture for stating cultivar is protected from light culture under the conditions of being 22~25 DEG C.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934586A (en) * | 2012-11-22 | 2013-02-20 | 福建省农业科学院食用菌研究所 | Low-carbon and high-yield poria culture method |
| CN105165392A (en) * | 2015-08-24 | 2015-12-23 | 重庆市药物种植研究所 | Artificial cultivation method for stone-like omphalia |
| CN105557303A (en) * | 2015-12-16 | 2016-05-11 | 新汇生物科技（梅州）有限公司 | Indoor precise cultivation method of pleurotus tuber regium |
| CN105900685A (en) * | 2016-05-05 | 2016-08-31 | 甄永福 | Grifola umbellate and pteridium aquilinum mixed planting method |
-
2019
- 2019-01-07 CN CN201910012112.1A patent/CN109588210A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934586A (en) * | 2012-11-22 | 2013-02-20 | 福建省农业科学院食用菌研究所 | Low-carbon and high-yield poria culture method |
| CN105165392A (en) * | 2015-08-24 | 2015-12-23 | 重庆市药物种植研究所 | Artificial cultivation method for stone-like omphalia |
| CN105557303A (en) * | 2015-12-16 | 2016-05-11 | 新汇生物科技（梅州）有限公司 | Indoor precise cultivation method of pleurotus tuber regium |
| CN105900685A (en) * | 2016-05-05 | 2016-08-31 | 甄永福 | Grifola umbellate and pteridium aquilinum mixed planting method |
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