CN109580882A - Device and its measuring method of the different fertilizer to same strain plant root effects - Google Patents
Device and its measuring method of the different fertilizer to same strain plant root effects Download PDFInfo
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- CN109580882A CN109580882A CN201811484986.9A CN201811484986A CN109580882A CN 109580882 A CN109580882 A CN 109580882A CN 201811484986 A CN201811484986 A CN 201811484986A CN 109580882 A CN109580882 A CN 109580882A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 76
- 241000196324 Embryophyta Species 0.000 title claims abstract description 59
- 230000000694 effects Effects 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims abstract description 37
- 239000002609 medium Substances 0.000 claims abstract description 14
- 235000013311 vegetables Nutrition 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims abstract description 8
- 229920001817 Agar Polymers 0.000 claims description 7
- 239000008272 agar Substances 0.000 claims description 7
- 238000011161 development Methods 0.000 claims description 7
- 238000005259 measurement Methods 0.000 claims description 7
- 230000018109 developmental process Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000004080 punching Methods 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 4
- 230000008635 plant growth Effects 0.000 claims description 4
- 239000012297 crystallization seed Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 230000035784 germination Effects 0.000 claims description 3
- 238000005192 partition Methods 0.000 claims description 3
- 238000007711 solidification Methods 0.000 claims description 3
- 230000008023 solidification Effects 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- 238000002844 melting Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract description 4
- 230000002786 root growth Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000010496 root system development Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000022116 gravitropism Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000027555 hydrotropism Effects 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008638 plant developmental process Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Hydroponics (AREA)
Abstract
The present invention discloses different fertilizer to the device of same strain plant root effects, including culture vessel, baffle is fixed on the inner wall of culture vessel, baffle will carry out trisection inside culture vessel, hollow section is set as different fertilizer to the observation area of same strain plant root effects after baffle trisection, the center of culture vessel offers putting hole by aseptic card punch, the culture medium for being embedded with vegetable seeds is contained inside putting hole, the bottom surface of observation area was provided with the circular hole that aseptic card punch opens up, the fertilizer cake that different fertilizer is made into is placed inside circular hole, avoid the individual difference in test, the interference of fertilizer and medium, keep experimental result more accurate, simultaneously, since culture medium is transparent can be monitored in real time to the growing state of root system of plant, this method can be suitable for solid fertilizer and liquid fertilizer simultaneously, it is one The good innovation scheme of kind, is very promising for marketing..
Description
Technical field
The present invention relates to the detection technique of root system of plant, more particularly to different fertilizer to the dress of same strain plant root effects
It sets and its measuring method.
Background technique
Root system plays a significant role in plant growth and development process as the major organs of plant absorption nutrition, in order to just
Continuous observation, which is carried out, in the growth and development situation to root system of plant needs a kind of device that can carry out plant root system development observation.
The device about root system development observation is concentrated mainly on water planting and culture vessel setting observation window at present, these can be by saturating
Bright medium is observed the growing state of root system of plant, while can also survey to a kind of growing state of fertilizer to plant
It is fixed.
Even there is also individual difference, above-mentioned devices can not carry out to same strain plant a variety of solid for same plant
Body fertilizer Preference and safety detection, these all extend the detection cycle of fertilizer, limit the progress of fertilizer industry.
In addition, these devices can not also be measured the effect of Water soluble fertilizer and strain secretion to same strain root system of plant, there is not
Foot, is not able to satisfy the demand of social development.
In conclusion in view of the drawbacks of the prior art, it is accordingly required in particular to dress of the different fertilizer to same strain plant root effects
It sets and its measuring method, so as to solve the deficiencies in the prior art.
Summary of the invention
Aiming at the problems existing in the prior art, measurement in practice is influenced, the present invention proposes different fertilizer to same strain
The device and its measuring method of plant root effects, novel in design, by the way that baffle is arranged in culture vessel, barrier small molecule exists
Infiltration between culture medium observes same strain root system of plant containing the growing state in different fertilizer culture medium, and practical performance is excellent,
The defect of the prior art is solved.
To achieve the goals above, technical scheme is as follows:
Different fertilizer is to the devices of same strain plant root effects, including culture vessel, culture vessel are transparence, culture
Baffle is fixed on the inner wall of container, baffle will carry out trisection, hollow section setting after baffle trisection inside culture vessel
It is different fertilizer to the observation area of same strain plant root effects, the center of culture vessel is offered by aseptic card punch
Putting hole, putting hole inside contain the culture medium for being embedded with vegetable seeds, and culture medium uses transparent solid culture medium, observation area
Bottom surface was provided with the circular hole that aseptic card punch opens up, and placed the fertilizer cake that different fertilizer is made into inside circular hole, between circular hole
Diameter it is identical.
Further, the height of the baffle and the height of culture vessel are identical.
Useful glass or PVC baffle are adopted in baffle of the present invention.
Further, the circular hole observation area to open up position identical.
Further, the shape of the observation area is fan-shaped.
Different fertilizer the steps include: the measuring method of same strain plant root effects
A. vegetable seeds is impregnated into sterile distilled water 10-30h, breaks in the seed coat until seed supports, then support is broken in the seed coat
Seeded dispersion be embedded in agar medium, will embed seed-bearing culture medium and be placed on temperature is 15-36 DEG C of constant temperature incubation
It is cultivated in case, temperature can be adjusted according to different seeds, to germination；
B. the culture medium to have sterilized is injected into the culture vessel with partition simultaneously, culture medium thickness can be according to crop root
Size carry out appropriate adjustment；
C. after culture medium solidification, using aseptic card punch, 1 putting hole is made a call in heart position in the medium, then uses and beats
Hole device will be embedded with the culture medium of vegetable seeds, be put into the putting hole at culture vessel center；
D. culture vessel is put into the constant incubator that temperature is 15-36 DEG C and is cultivated, and according to circumstances supplement light
According to growing root system to plant；
E. fertilizer is made into fertilizer cake；
F. after plant grows root system, circular hole can be beaten using punch in the observation area of trisection, then by fertilizer
Cake is put into circular hole；
G. continue to cultivate plant, can be compared in three regions through the development of observation area measurement root system of plant
The developmental state of root system, to determine influence of three kinds of fertilizer to plant growth.
Further, the length that the present invention is germinateed and the half for being seed length.
Further, the method that the present invention makes cake fertilizer are as follows: take fertilizer 3-5g to be mixed with the culture medium for melting agar, to
Fertilizer cake is obtained using punch punching after mixture cooled and solidified.
The beneficial effects of the present invention are: novel in design, structure is simple, operate it is convenient, avoid test in individual difference,
The interference of fertilizer and medium keeps experimental result more accurate, simultaneously as culture medium be it is transparent can be in real time to root system of plant
Growing state be monitored, be convenient for the influence to plant root growth situation of screening and measurement strain of fertilizer formula,
The measurement period is also shortened, testing cost is reduced, reduces workload, improves working efficiency, in addition to this, this method can be same
When be suitable for solid fertilizer and liquid fertilizer, be a kind of good innovation scheme, be very promising for marketing..
Detailed description of the invention
The following describes the present invention in detail with reference to the accompanying drawings and specific embodiments:
Fig. 1 is planar structure schematic diagram of the invention；
Fig. 2 is schematic perspective view of the invention.
100- culture vessel in figure, 110- baffle, the observation area 120-, 130- putting hole, 140- circular hole.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Conjunction is specifically illustrating, and the present invention is further explained.
Referring to Fig. 1, Fig. 2, different fertilizer holds the devices of same strain plant root effects, including culture vessel 100, culture
Device 100 is transparence, is fixed with baffle 110 on the inner wall of culture vessel 100, baffle 110 will carry out three inside culture vessel 100
Equal part, baffle 110 set different fertilizer to the observation area 120 of same strain plant root effects for section hollow after trisection,
The center of culture vessel 100 offers putting hole 130 by aseptic card punch, contains inside putting hole 130 and is embedded with plant
The culture medium of species, culture medium use transparent solid culture medium, and the bottom surface of observation area 130 was provided with aseptic card punch and opens
If circular hole 140, the fertilizer cake that different fertilizer is made into is placed inside circular hole 140, the diameter between circular hole 140 is identical.
In addition, the height of baffle 110 is identical with the height of culture vessel 100.Baffle 120 adopts useful glass or PVC baffle.
Circular hole 140 opens up that position is identical observation area, and the shape of observation area 140 is fan-shaped.The device is using transparent culture medium to plant
Object is cultivated, and can clearly be observed root growth and development situation.The transparent baffle in culture apparatus simultaneously, can be with
Stop the diffusion of fertilizer small molecular in the medium, reduces interfering with each other between different fertilizer.
The present invention utilizes transparent solid culture medium, is monitored to the growing state of root system of plant.Simultaneously in culture vessel
Middle setting glass or PVC baffle obstruct infiltration of the small molecule between culture medium.By observing same strain root system of plant containing not
With the growing state in the culture medium of fertilizer, to determine whether these substances have the work of the root system development and growth that promote plant
With.In order to detect one plant of plant for the preference profile of different fertilizer, need this device can be simultaneously to two or more fertilizer
Expect that the growth to root system of plant promotes situation to compare.In addition, with the development of Water soluble fertilizer and bacterial manure industry, in fertilizer formula
In terms of bacterial screening, which can all play a significant role.
The present invention utilizes the characteristics of plant tropic movement, i.e. root system of plant has gravitropism, chmotropism and hydrotropism, row
Except gravitropism and hydrotropism, the root system of plant has grown always more towards fertilizer in soil, so that fertilizer is more
Region well developed root system.
The embodiment of the present invention are as follows:
Vegetable seeds is impregnated 10-30h by the first step in sterile distilled water, and until seed support is broken in the seed coat, seed is using oil
Colza.Then the agar medium of thawing is poured in culture dish, when culture medium will solidify, seed that support is broken in the seed coat
Embedding is in the medium, it is noted that temperature does not exceed 50 when embedding.Seed-bearing culture dish will be embedded and use preservative film or culture
Ware lid sealing is placed in the constant incubator that temperature is 15-36 DEG C and cultivates, and temperature can be adjusted according to different seeds, to seed
Germination, the length of bud and the half for being seed length.
The agar medium to have sterilized is injected the culture vessel with partition by second step, and culture medium thickness can be according to work
The size of object root system carries out appropriate adjustment.
Third step makes a call to 1 in the medium heart position (at D) using aseptic card punch after culture medium solidification in container
Circular hole is taken out cylindrical culture medium, the culture medium of vegetable seeds then will be embedded with using punch, is put into culture vessel center
(D) in circular hole.
4th step is put into the constant incubator that temperature is 15-36 DEG C after sealing culture vessel and cultivates, and according to
Situation supplementary light grows root system to plant.Period pays attention to keeping the skin wet.
5th step makes into fertilizer fertilizer cake.The agar medium for taking liquid fertilizer fertilizer 3-5g and 200mL to melt carries out
It mixes, obtains fertilizer cake using punch punching after object cooled and solidified to be mixed.
6th step can be punched in 3 positions of A, B, C using punch, after plant grows root system then by fertilizer
Cake is put into circular hole.
7th step continues to cultivate plant, can penetrate the development of transparent culture medium observation of plant root system.Comparison 3
The developmental state of root system can determine influence of 3 kinds of fertilizer to plant growth in a region.
The beneficial effects of the present invention are: novel in design, structure is simple, operate it is convenient, avoid test in individual difference,
The interference of fertilizer and medium keeps experimental result more accurate, simultaneously as culture medium be it is transparent can be in real time to root system of plant
Growing state be monitored, be convenient for the influence to plant root growth situation of screening and measurement strain of fertilizer formula,
The measurement period is also shortened, testing cost is reduced, reduces workload, improves working efficiency, in addition to this, this method can be same
When be suitable for solid fertilizer and liquid fertilizer, be a kind of good innovation scheme, be very promising for marketing..
It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, above-described embodiment and explanation
It is merely illustrated the principles of the invention described in book, the present invention also has each without departing from the spirit and scope of the present invention
Kind changes and improvements, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention by
Appended claims and its equivalent define.
Claims (8)
1. different fertilizer is to the device of same strain plant root effects, including culture vessel, it is characterised in that: culture vessel is
Bright shape, is fixed with baffle on the inner wall of culture vessel, and baffle will carry out trisection inside culture vessel, hollow after baffle trisection
Section is set as different fertilizer to the observation area of same strain plant root effects, and the center of culture vessel passes through sterile punching
Device offers putting hole, and the culture medium for being embedded with vegetable seeds is contained inside putting hole, and culture medium uses transparent solid culture medium,
The bottom surface of observation area was provided with the circular hole that aseptic card punch opens up, and the fertilizer cake that different fertilizer is made into is placed inside circular hole,
Diameter between circular hole is identical.
2. device of the different fertilizer to same strain plant root effects according to claim 1, it is characterised in that: the baffle
Height it is identical with the height of culture vessel.
3. device of the different fertilizer to same strain plant root effects according to claim 1, it is characterised in that: the baffle
Adopt useful glass or PVC baffle.
4. device of the different fertilizer to same strain plant root effects according to claim 1, it is characterised in that: the circular hole
Observation area to open up position identical.
5. device of the different fertilizer to same strain plant root effects according to claim 1, it is characterised in that: the observation
The shape in area is fan-shaped.
6. different fertilizer is to the measuring method of same strain plant root effects, it is characterised in that: the steps include:
A. vegetable seeds is impregnated into sterile distilled water 10-30h, broken in the seed coat until seed supports, the kind that then support is broken in the seed coat
Son dispersion is embedded in agar medium, will be embedded seed-bearing culture medium and is placed in the constant incubator that temperature is 15-36 DEG C
Culture, temperature can be adjusted according to different seeds, to germination；
B. the culture medium to have sterilized is injected into the culture vessel with partition simultaneously, culture medium thickness can be according to the big of crop root
Small carry out appropriate adjustment；
C. after culture medium solidification, using aseptic card punch, 1 putting hole is made a call in heart position in the medium, then uses punch
It will be embedded with the culture medium of vegetable seeds, is put into the putting hole at culture vessel center；
D. culture vessel is put into the constant incubator that temperature is 15-36 DEG C and is cultivated, and according to circumstances supplementary light, to
Plant grows root system；
E. fertilizer is made into fertilizer cake；
F. after plant grows root system, circular hole can be beaten using punch in the observation area of trisection, then puts fertilizer cake
Enter in circular hole；
G. continue to cultivate plant, root system in three regions can be compared through the development of observation area measurement root system of plant
Developmental state, to determine influence of three kinds of fertilizer to plant growth.
7. measuring method of the different fertilizer to same strain plant root effects according to claim 1, it is characterised in that: germination
Length be seed length half.
8. measuring method of the different fertilizer to same strain plant root effects according to claim 1, it is characterised in that: production
The method of cake fertilizer are as follows: take fertilizer 3-5g to be mixed with the culture medium for melting agar, punching is used after object cooled and solidified to be mixed
Device punching obtains fertilizer cake.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811484986.9A CN109580882A (en) | 2018-12-06 | 2018-12-06 | Device and its measuring method of the different fertilizer to same strain plant root effects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811484986.9A CN109580882A (en) | 2018-12-06 | 2018-12-06 | Device and its measuring method of the different fertilizer to same strain plant root effects |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109580882A true CN109580882A (en) | 2019-04-05 |
Family
ID=65926156
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811484986.9A Pending CN109580882A (en) | 2018-12-06 | 2018-12-06 | Device and its measuring method of the different fertilizer to same strain plant root effects |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973612A (en) * | 2021-10-27 | 2022-01-28 | 华南农业大学 | Plant root division observation device and method |
| CN114062599A (en) * | 2021-11-04 | 2022-02-18 | 西交利物浦大学 | Device and method for toxicology research of plant roots |
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| CN101658107A (en) * | 2009-09-18 | 2010-03-03 | 华南农业大学 | Method for in-situ dynamic observation and measurement of root system of plant |
| CN102763573A (en) * | 2012-07-05 | 2012-11-07 | 华南农业大学 | Plant root system in-situ dynamic observation device, system and method under soil culture conditions |
| CN102783417A (en) * | 2012-08-06 | 2012-11-21 | 云南农业大学 | Method for using glass tube for culturing straight root system plants for directly measuring root length in different stages |
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| CN103960038A (en) * | 2014-05-29 | 2014-08-06 | 福建农林大学 | Test device and method for researching root competition strategy |
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| CN206728672U (en) * | 2017-03-10 | 2017-12-12 | 广州花云网络科技有限公司 | plant root growth test container |
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2018
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973612A (en) * | 2021-10-27 | 2022-01-28 | 华南农业大学 | Plant root division observation device and method |
| CN114062599A (en) * | 2021-11-04 | 2022-02-18 | 西交利物浦大学 | Device and method for toxicology research of plant roots |
| CN114062599B (en) * | 2021-11-04 | 2024-06-07 | 西交利物浦大学 | Device and method for researching toxicology of plant roots |
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