CN109580756A - Composition applied to Capillary Electrophoresis - Google Patents
Composition applied to Capillary Electrophoresis Download PDFInfo
- Publication number
- CN109580756A CN109580756A CN201811463721.0A CN201811463721A CN109580756A CN 109580756 A CN109580756 A CN 109580756A CN 201811463721 A CN201811463721 A CN 201811463721A CN 109580756 A CN109580756 A CN 109580756A
- Authority
- CN
- China
- Prior art keywords
- poly
- methyl
- acrylamide
- amide
- propen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Abstract
The present invention relates to the compositions for being applied to Capillary Electrophoresis, and the composition includes quasi- interpenetrating polymer, denaturant and buffer；The quasi- interpenetrating polymer includes polymer, the water soluble polymer with the acrylamide derivative monomer of hydrophobic group.The beneficial effect that the present invention reaches is that Capillary Electrophoresis is strong from coating, separation analytical effect is excellent.
Description
Technical field
The present invention relates to capillary electrophoresis technique fields, are especially applied to the composition of Capillary Electrophoresis.
Background technique
Capillary Electrophoresis is charge species under the action of electric field, the other end is moved to from one end of capillary, using this
The rapidly and efficiently separation of the mixture especially large biological molecule of different charged particles may be implemented in one technology.
The essence of capillary electrophoresis separation charge species is the interaction of power between medium and capillary and separated object,
Its active force mainly includes three aspects: first is that longitudinal electric field driven power, second is that lateral capillary wall adsorption capacity, third is that electric
Longitudinal movement resistance of the swimming medium to separated object matter；Realize that efficiently separating for charge species must shield capillary wall as far as possible
The lateral of separated object matter is adsorbed and allow separated object matter longitudinal movement time enough under electric field force effect；Therefore it selects
Electrophoretic medium must have to capillary tube inner wall and have stronger coating effect to eliminate tube wall to the transverse direction of separated object matter and adsorb,
Reduce electroosmotic flow, increases medium comparative resistance；In terms of qualified sieving media must take into account three above.
Common macromolecule separating medium is polyacrylamide, can with isolation of DNA fragments up to 1200 base numbers, but poly- third
Acrylamide does not have shielding action to capillary wall, it is necessary to add coating in capillary tube inner wall, eliminate capillary tube inner wall to being divided
From large biological molecule absorption and reduce electroosmotic flow, can just efficiently separate large biological molecule especially DNA fragmentation.
Chinese patent document CN102220599A discloses a kind of quasi- interpenetrating polymerization of capillary gel electrophoresis sieving media
The preparation method and purposes of object network, the invention provide a kind of long-chain, short chain linearly poly- N,N-DMAA and
Polyacrylamide-N,N-DMAA ignores that copolymer is entangled with one another, interpenetrating, forms the quasi- interpenetrating polymerization of polymer chain
Object grid.But the quasi- interpenetrating polymer networks of polymer chain formed in this way, stability is poor, resolution ratio is low, unfavorable
It is analyzed in electrophoretic separation.
Detailed description of the invention
Fig. 1 is that the quasi- interpenetrating polymer of polymethacrylamide-ethylene polyethenoxy ether prepares correspondent composition
LIZ500 molecular weight internal standard detection effect figure；
Fig. 2 is the ID- that the quasi- interpenetrating polymer of polymethacrylamide-ethylene polyethenoxy ether prepares correspondent composition
The allelic ladder LADDER detection effect figure of PLUS；
Fig. 3 is the LIZ500 for preparing correspondent composition points of the poly- quasi- interpenetrating polymer of N methacrylamide-polyvinyl alcohol
Son amount internal standard detection effect figure；
Fig. 4 is the ID-PLUS's for preparing correspondent composition of the poly- quasi- interpenetrating polymer of N methacrylamide-polyvinyl alcohol
Allelic ladder LADDER detection effect figure.
Summary of the invention
The purpose of the present invention is to overcome the shortcomings of the existing technology, provide molecular weight distribution gradient it is big, it is strong from paintability,
The good composition applied to Capillary Electrophoresis of stability.
The purpose of the present invention is achieved through the following technical solutions: applied to the composition of Capillary Electrophoresis, the group
Closing object includes quasi- interpenetrating polymer (mixture), denaturant and buffer；
The quasi- interpenetrating polymer includes the polymer with the acrylamide derivative monomer of hydrophobic group, water-soluble high score
Son, the polymer of the acrylamide derivative monomer with hydrophobic group are the acrylamide derivative monomer polymerization with hydrophobic group
It forms.
The acrylamide derivative monomer with hydrophobic group includes but is not limited to following one or more:
N-2- cyanoethyl-acrylamide, N, N-1,2- dihydroxy ethylidene-bis--acrylamide, N-4,4- dimethoxy fourth
Base-acrylamide, N-2,2- dimethoxy-ethyl-Methacrylamide, N,N-DMAA, N-2-- hydroxyacetic acid
Methyl esters acrylamide, N-2-- ethoxy-Methacrylamide, N- methylol-Methacrylamide, N- methoxy-methyl
Acrylamide, N-3- methoxy-propyl-acrylamide, N- methyl-propen amide, N- methyl-N-2,2- dimethoxy-ethyl-the third
Acrylamide, N- morpholine-ethyl-acrylamide, N-2,2,2- tri- chloro- 1- ethoxy-acrylamides, Methacrylamide, N- second
Acyl group-methyl-propen amide, N-2- cyanoethyl-methyl-propen amide, NN-1,2- dihydroxy ethylidene-bis--methyl-propen
Amide, N-4,4- dimethylbutyl-Methacrylamide, N-2,2- dimethoxy-ethyl-methyl-propen amide, N, N- diformazan
Base-methyl-propen amide, N-2- hydroxy methyl acetate-methyl-propen amide, N-2- ethoxy-methyl-propen amide, N- hydroxyl
Methyl-methyl-propen amide, N- methoxy-methyl-propen amide, N-3- methoxy-propyl-methyl-propen amide, N-
Methyl-methyl-propen amide, N- methyl-N-2,2- dimethoxy-ethyl-Methacrylamide, N- morpholine-Ethyl-Methyl third
Acrylamide, N-2,2,2- tri- chloro- 1- ethoxy-Methacrylamides.
The weight-average molecular weight of the polymer of the acrylamide derivative monomer with hydrophobic group be 10MDA~
15MDA。
The weight-average molecular weight of the water soluble polymer is 10MDA~50MDA, including following polymer or it is mixed
Close object: polybutene amide, poly- N- acetylacrylamide, poly- N-2- cyanoethyl-acrylamide, poly- N, N-1,2- dihydroxy Asia second
Base-is bis--acrylamide, poly- N-4,4- dimethoxybutyl-acrylamide, and poly- N-2,2- dimethoxy-ethyl-methacryl
Amine, poly- N,N-DMAA, poly- N-2-- hydroxy methyl acetate acrylamide, poly- N-2-- ethoxy-methacryl
Amine, poly- N- methylol-Methacrylamide, poly- N- methoxy-Methacrylamide, poly- N-3- methoxy-propyl-propylene
Amide, poly- N- methyl-propen amide, poly- N- methyl-N-2,2- dimethoxy-ethyl-acrylamide, poly- N- morpholine-ethyl-the third
Acrylamide, poly- N-2,2,2- tri- chloro- 1- ethoxy-acrylamides, polymethacrylamide, poly- N- acetyl group-methyl-propen acyl
Amine, poly- N-2- cyanoethyl-methyl-propen amide, poly- NN-1,2- dihydroxy ethylidene-bis--methyl-propen amide, poly- N-4,4-
Dimethylbutyl-Methacrylamide, poly- N-2,2- dimethoxy-ethyl-methyl-propen amide, poly- N, N- dimethyl-methyl-
Acrylamide, poly- N-2- hydroxy methyl acetate-methyl-propen amide, poly- N-2- ethoxy-methyl-propen amide, poly- N- hydroxyl first
Base-methyl-propen amide, poly- N- methoxy-methyl-propen amide, poly- N-3- methoxy-propyl-methyl-propen amide,
Poly- N- methyl-methyl-propen amide, poly- N- methyl-N-2,2- dimethoxy-ethyl-Methacrylamide, poly- N- morpholine-second
Base-Methacrylamide, poly- N-2,2,2- tri- chloro- 1- ethoxy-Methacrylamides, poly- hydroxy methylene, polyoxyethylene gather
Oxypropylene, polyvinyl alcohol, polyvinylpyrrolidone, poly- 2- ethyl -2- oxazoline, poly- 2- ethyl -2- oxazoline, poly- N- acetyl
Amino acryl amides, polypropylene oxygroup urea, hydroxyethyl cellulose, hydroxymethyl cellulose, ethylene polyethenoxy ether, polyvinyl alcohol
Propylene ether.
The denaturant includes one or both of urea, formamide.
The buffer includes Tris base, two or more in EDTA, TAPS.
The composition PH is 6 to 10.
The composition carries out the separation analysis of biomolecules mixture for capillary electrophoresis.
The invention has the following advantages that (1) invention introduces water soluble polymer, allows the acrylamide with hydrophobic group
Derivative monomer and water soluble polymer are indissoluble in the form of quasi- interpenetrating polymer networks, have adjusted the flat of quasi- interpenetrating polymer
Average molecular weight；(2) present invention introduces the acrylamide derivatives with hydrophobic group enhances polymer since hydrophobic bond increases
With the absorption of capillary inner surface, therefore quasi- interpenetrating polymer sieving media of the invention have preferably from coating property；(3)
The amide group of acrylamide derivative has good sieving actoion, therefore third with hydrophobic group to large biological molecule simultaneously
Acrylamide derived polymer will fully meet sieving media to separation of biopolymer point from coating property in conjunction with sieving actoion
The technical requirements of analysis.
Specific embodiment
The present invention will be further described below, but protection scope of the present invention be not limited to it is as described below.
Composition applied to Capillary Electrophoresis:
The composition includes quasi- interpenetrating polymer, denaturant and buffer；
The quasi- interpenetrating polymer includes the polymer with the acrylamide derived polymer monomer of hydrophobic group, water-soluble
Property macromolecule, the polymer of the acrylamide derivative monomer with hydrophobic group is the acrylamide derivative with hydrophobic group
Monomer polymerization forms.
Wherein the weight-average molecular weight of the acrylamide derived polymer with hydrophobic group for 10MDA~
15MDA, the acrylamide derivative monomer with hydrophobic group have following structure formula:
Each R1, R2, R3 group independently be or comprising-H ,-OH ,-(C1-C8 alkyl) ,-(C3-C8 naphthenic base) ,-(fragrance
Base), 5-10 unit's heteroaryl ,-(C1-C10 alkyl) (aromatic radical) or-(aromatic radical) (C1-C10 alkyl) ,-(C1-C4 alkyl)
NHCONH2 ,-(C1-C4 alkyl) NHCOH ,-(C1-C4 alkyl) NHCOCH3.
Specifically, the acrylamide derivative monomer with hydrophobic group includes but is not limited to following one kind or more
Kind: N-2- cyanoethyl-acrylamide, N, N-1,2- dihydroxy ethylidene-bis--acrylamide, N-4,4- dimethoxybutyl-the third
Acrylamide, N-2,2- dimethoxy-ethyl-Methacrylamide, N,N-DMAA, N-2-- hydroxy methyl acetate third
Acrylamide, N-2-- ethoxy-Methacrylamide, N- methylol-Methacrylamide, N- methoxy-methacryl
Amine, N-3- methoxy-propyl-acrylamide, N- methyl-propen amide, N- methyl-N-2,2- dimethoxy-ethyl-acryloyl
Amine, N- morpholine-ethyl-acrylamide, N-2,2,2- tri- chloro- 1- ethoxy-acrylamides, Methacrylamide, N- acetyl group-
Methyl-propen amide, N-2- cyanoethyl-methyl-propen amide, NN-1,2- dihydroxy ethylidene-bis--methyl-propen amide,
N-4,4- dimethylbutyl-Methacrylamide, N-2,2- dimethoxy-ethyl-methyl-propen amide, N, N- dimethyl-first
Base-acrylamide, N-2- hydroxy methyl acetate-methyl-propen amide, N-2- ethoxy-methyl-propen amide, N- methylol-
Methyl-propen amide, N- methoxy-methyl-propen amide, N-3- methoxy-propyl-methyl-propen amide, N- methyl-
Methyl-propen amide, N- methyl-N-2,2- dimethoxy-ethyl-Methacrylamide, N- morpholine-Ethyl-Methyl acryloyl
Amine, N-2,2,2- tri- chloro- 1- ethoxy-Methacrylamides.
Further, the weight-average molecular weight of the water soluble polymer is 10MDA~50MDA, water-soluble high score
Attached bag includes below one or more:
Polybutene amide, poly- N- acetylacrylamide, poly- N-2- cyanoethyl-acrylamide, poly- N, N-1,2- dihydroxy
Ethylidene-is bis--acrylamide, poly- N-4,4- dimethoxybutyl-acrylamide, and poly- N-2,2- dimethoxy-ethyl-methyl-prop
Acrylamide, poly- N,N-DMAA, poly- N-2-- hydroxy methyl acetate acrylamide, poly- N-2-- ethoxy-methyl-prop
Acrylamide, poly- N- methylol-Methacrylamide, poly- N- methoxy-Methacrylamide, poly- N-3- methoxy-propyl-
Acrylamide, poly- N- methyl-propen amide, poly- N- methyl-N-2,2- dimethoxy-ethyl-acrylamide, poly- N- morpholine-second
Base-acrylamide, poly- N-2,2,2- tri- chloro- 1- ethoxy-acrylamides, polymethacrylamide, poly- N- acetyl group-methyl-
Acrylamide, poly- N-2- cyanoethyl-methyl-propen amide, poly- NN-1,2- dihydroxy ethylidene-bis--methyl-propen amide are gathered
N-4,4- dimethylbutyl-Methacrylamide, poly- N-2,2- dimethoxy-ethyl-methyl-propen amide, poly- N, N- diformazan
Base-methyl-propen amide, poly- N-2- hydroxy methyl acetate-methyl-propen amide, poly- N-2- ethoxy-methyl-propen amide,
Poly- N- methylol-methyl-propen amide, poly- N- methoxy-methyl-propen amide, poly- N-3- methoxy-propyl-methyl-
Acrylamide, poly- N- methyl-methyl-propen amide, poly- N- methyl-N-2,2- dimethoxy-ethyl-Methacrylamide, poly- N-
Morpholine-Ethyl-Methyl acrylamide, poly- N-2,2,2- tri- chloro- 1- ethoxy-Methacrylamides, poly- hydroxy methylene, polyoxy
Ethylene, polyoxypropylene, polyvinyl alcohol, polyvinylpyrrolidone, poly- 2- ethyl -2- oxazoline, poly- 2- ethyl -2- oxazoline,
Poly- N- acetamidoacrylamide, polypropylene oxygroup urea, hydroxyethyl cellulose, hydroxymethyl cellulose, ethylene polyethenoxy ether,
Polyvinyl alcohol propylene ether.
In the present embodiment, the denaturant includes one or both of urea, formamide；
In the present embodiment, the composition includes Tris base, two or more in EDTA, TAPS；
In the present embodiment, the composition PH is 6 to 10；
In the present embodiment, the method for preparing quasi- interpenetrating polymer are as follows:
Water-soluble high-molecular compound (buying from market) is mixed with the acrylamide derivative monomer with hydrophobic group
It is dissolved in solvent and is added initiator and chain-transferring agent, moderately heating or radiation exposure complete polymerization reaction under oxygen-free environment,
Product is through dialysis purification, freeze-dried back；It is micro- that the polymerization system of the reaction can be solution, suspension, lotion or lotion
Grain, initiator can be free radical type such as azo-compound, diazonium compound, organic peroxide, organic hydroperoxide, have
Machine persulfate, organic hydrogen persulfate, inorganic peroxide, inorganic peroxysulfate, peroxide-redox systems, light rise
Or mixtures thereof beginning agent；Chain-transferring agent can be isopropanol, ethyl alcohol, sodium formate etc..
In the present embodiment, application and preparation is in the method for the composition of Capillary Electrophoresis are as follows:
It is at 25 DEG C that quasi- interpenetrating polymer (or its salt) of the invention is soluble in water, the denaturation of conc forms is then added
Agent, buffer；Denaturant selects urea or formamide, and buffer selects Tris base, two or more in EDTA, TAPS.
If selecting Tris base, molar concentration is in 0.05M between 0.12M；If selecting TAPS, molar concentration is in 0.05M
To between 0.07M；If selecting EDTA, molar concentration is in 0.001M between 0.0.005M.
[embodiment one]
Now selecting the acrylamide derivative with hydrophobic group is Methacrylamide, and water soluble polymer uses ethylene glycol
Polyoxyethylene ether prepares quasi- interpenetrating polymer, and preparing the resulting quasi- polymer that mutually passes is mixture, and preparation condition is detailed in table one.
Phase is prepared using the quasi- interpenetrating polymer of polymethacrylamide-ethylene polyethenoxy ether made from one method of table again
The composition answered, preparation condition are detailed in table two: above-mentioned quasi- interpenetrating polymer is dissolved in the solution of buffer and denaturant at 25 DEG C
In prepare the composition, 10 × TTE is Tris base (molar concentration 1M), TAPS (molar concentration 0.7M), EDTA
(molar concentration 0.02M) and pure water composition.
One polymethacrylamide of table-ethylene polyethenoxy ether is quasi- mutually to pass polymer polymerizing condition
Table dimethylacrylamide-quasi- interpenetrating polymer of polyoxyethylene prepares the condition of correspondent composition
| Composition title | Dosage |
| The quasi- interpenetrating polymer of polymethacrylamide-ethylene polyethenoxy ether | 10.1g |
| 10 × TTE buffer | 11ml |
| Urea | 90g |
| Pure water | 100ml |
In order to detect the composition of the quasi- interpenetrating polymer preparation of polymethacrylamide-ethylene polyethenoxy ether in table two
Performance, now detected by two ways.
Detection mode one:
Detection to LIZ500 molecular weight internal standard (being purchased from American AB company):
Capillary electrophoresis separation detection is carried out using 3130 type genetic analyzer of AB；The condition of capillary electrophoresis detection is:
Voltage is 15kV, sample injection time 10s, capillary pipe length 36cm, and electrophoresis temperature is 60 DEG C；Illustrate to carry out according to instrumentation
Capillary electrophoresis detection testing result is as shown in Figure 1；It can be seen from the figure that LIZ500 peak type is sharp, separating degree is good, without miscellaneous
Peak, non-specific peak and without trailing phenomenon, fully meet the separation analysis needs of LIZ500.
Detection mode two:
Detection to the allelic ladder LADDER (being purchased from American AB company) of ID-PLUS:
Capillary electrophoresis separation detection is carried out using 3130 type genetic analyzer of AB；The condition of capillary electrophoresis detection is:
Voltage 15kV, sample injection time are 15s, and capillary pipe length is 36cm, and electrophoresis temperature is 60 DEG C, illustrate to carry out hair according to instrumentation
Cons electrophoresis detection, electrophoresis result such as Fig. 2 show that Ladder peak type is sharp, and separating degree is good, can be realized THO1 locus
9.3bp/10bp neighboring gene copy efficiently separates, and no miscellaneous peak, non-specific peak and without trailing phenomenon fully meet DNA piece
The separation of section, which is analyzed, to be needed.
[embodiment 2]
Now selecting the acrylamide derivative with hydrophobic group is N methacrylamide, and water soluble polymer uses polyoxy
Ethylene prepares quasi- interpenetrating polymer, and preparing the resulting quasi- polymer that mutually passes is mixture, and preparation condition is detailed in table three.
Again using the poly- quasi- interpenetrating polymer of N methacrylamide-polyoxyethylene made from table three, corresponding combination is prepared
Object.At 25 DEG C the composition will be prepared in solution that interpenetrating polymer quasi- in the present embodiment is dissolved in buffer and denaturant, 10
× TTE is Tris base (molar concentration 1M), TAPS (molar concentration 0.7M), EDTA (molar concentration 0.02M) group
At preparation condition such as table four.
The polymerizing condition of three N methacrylamide of table and the quasi- interpenetrating polymer of polyoxyethylene
The condition for preparing correspondent composition of the quasi- interpenetrating polymer of four N methacrylamides of table-polyvinyl alcohol
| Composition title | Dosage |
| The quasi- interpenetrating polymer of polymethacrylamide-polyvinyl alcohol | 10.1g |
| 10X TTE buffer | 11ml |
| Urea | 90g |
| Pure water | 100ml |
In order to detect in table four, the performance of the composition of the quasi- interpenetrating polymer preparation of polymethacrylamide-polyvinyl alcohol,
Now detected by two ways.
Detection mode one:
The detection of LIZ500 molecular weight internal standard (being purchased from American AB company) is carried out to it:
Capillary electrophoresis separation detection is carried out using 3130 type genetic analyzer of AB；The condition of capillary electrophoresis detection is:
Voltage is 15kV, sample injection time 10s, capillary pipe length 36cm, and electrophoresis temperature is 60 DEG C, illustrates to carry out according to instrumentation
Capillary electrophoresis detection, for testing result as shown in figure 3, LIZ500 peak type is sharp, separating degree is good, no miscellaneous peak, non-specific peak and
Without trailing phenomenon.Fully meet the separation analysis needs of DNA fragmentation.
Detection mode two:
The detection of the allelic ladder LADDER (being purchased from American AB company) of ID-PLUS is carried out to it:
Capillary electrophoresis separation detection is carried out using 3130 type genetic analyzer of AB；The condition of capillary electrophoresis detection is:
Electrophoretic voltage is 15kV, and sample injection time is 15s, and capillary pipe length is 36cm, and electrophoresis temperature is 60 DEG C, is illustrated according to instrumentation
Capillary electrophoresis detection is carried out, electrophoresis result such as Fig. 4 shows that Ladder peak type is sharp, and separating degree is good, can be realized THO1 gene
The 9.3bp/10bp neighboring gene copy of seat efficiently separates, and no miscellaneous peak, non-specific peak and without trailing phenomenon are fully met
The separation of DNA fragmentation, which is analyzed, to be needed.
Invention introduces water soluble polymer, acrylamide derivative monomer and water soluble polymer with hydrophobic group are allowed
It is indissoluble in the form of quasi- interpenetrating polymer networks, have adjusted the average molecular weight of quasi- interpenetrating polymer；Have by introducing
The acrylamide derivative of hydrophobic group enhances the absorption of polymer and capillary inner surface, therefore this since hydrophobic bond increases
The quasi- interpenetrating polymer sieving media of invention has preferably from coating property；The amide group pair of acrylamide derivative simultaneously
Large biological molecule have good sieving actoion, therefore with hydrophobic group acrylamide derived polymer will from coating property with
Sieving actoion combines, and fully meets the technical requirements that sieving media analyzes separation of biopolymer.
Although with reference to particular embodiment, the present invention is described, without departing substantially from spirit of the invention or
The various changes and improvement carried out in the case where range each fall within protection scope of the present invention.
Claims (8)
1. being applied to the composition of Capillary Electrophoresis, it is characterised in that: the composition includes quasi- interpenetrating polymer, denaturant
And buffer；
The quasi- interpenetrating polymer includes the polymer with the acrylamide derivative monomer of hydrophobic group, water-soluble high score
Son, the polymer of the acrylamide derivative monomer with hydrophobic group are the acrylamide derivative monomer polymerization with hydrophobic group
It forms.
2. the composition according to claim 1 applied to Capillary Electrophoresis, it is characterised in that: described has hydrophobic group
Acrylamide derivative monomer include but is not limited to following one or more monomers:
N-2- cyanoethyl-acrylamide, N, N-1,2- dihydroxy ethylidene-bis--acrylamide, N-4,4- dimethoxybutyl-
Acrylamide, N-2,2- dimethoxy-ethyl-Methacrylamide, N,N-DMAA, N-2-- hydroxyacetic acid first
Ester acrylamide, N-2-- ethoxy-Methacrylamide, N- methylol-Methacrylamide, N- methoxy-methyl-prop
Acrylamide, N-3- methoxy-propyl-acrylamide, N- methyl-propen amide, N- methyl-N-2,2- dimethoxy-ethyl-propylene
Amide, N- morpholine-ethyl-acrylamide, N-2,2,2- tri- chloro- 1- ethoxy-acrylamides, Methacrylamide, N- acetyl
Base-methyl-propen amide, N-2- cyanoethyl-methyl-propen amide, NN-1,2- dihydroxy ethylidene-bis--methyl-propen acyl
Amine, N-4,4- dimethylbutyl-Methacrylamide, N-2,2- dimethoxy-ethyl-methyl-propen amide, N, N- dimethyl-
Methyl-propen amide, N-2- hydroxy methyl acetate-methyl-propen amide, N-2- ethoxy-methyl-propen amide, N- hydroxyl first
Base-methyl-propen amide, N- methoxy-methyl-propen amide, N-3- methoxy-propyl-methyl-propen amide, N- first
Base-methyl-propen amide, N- methyl-N-2,2- dimethoxy-ethyl-Methacrylamide, N- morpholine-Ethyl-Methyl propylene
Amide, N-2,2,2- tri- chloro- 1- ethoxy-Methacrylamides.
3. the composition according to claim 2 applied to Capillary Electrophoresis, it is characterised in that: described has hydrophobic group
Acrylamide derivative monomer polymer weight-average molecular weight be 10MDA ~ 15MDA.
4. the composition according to claim 3 applied to Capillary Electrophoresis, it is characterised in that: the water-soluble high score
The weight-average molecular weight of son is or mixtures thereof 10MDA ~ 50MDA, including following high-molecular compound:
Polybutene amide, poly- N- acetylacrylamide, poly- N-2- cyanoethyl-acrylamide, poly- N, N-1,2- dihydroxy Asia second
Base-is bis--acrylamide, poly- N-4,4- dimethoxybutyl-acrylamide, and poly- N-2,2- dimethoxy-ethyl-methacryl
Amine, poly- N,N-DMAA, poly- N-2- hydroxy methyl acetate acrylamide, poly- N-2- ethoxy-Methacrylamide,
Poly- N- methylol-Methacrylamide, poly- N- methoxy-Methacrylamide, poly- N-3- methoxy-propyl-acryloyl
Amine, poly- N- methyl-propen amide, poly- N- methyl-N-2,2- dimethoxy-ethyl-acrylamide, poly- N- morpholine-ethyl-propylene
Amide, poly- N-2,2,2- tri- chloro- 1- ethoxy-acrylamides, polymethacrylamide, poly- N- acetyl group-methyl-propen acyl
Amine, poly- N-2- cyanoethyl-methyl-propen amide, poly- NN-1,2- dihydroxy ethylidene-bis--methyl-propen amide, poly- N-4,4-
Dimethylbutyl-Methacrylamide, poly- N-2,2- dimethoxy-ethyl-methyl-propen amide, poly- N, N- dimethyl-methyl-
Acrylamide, poly- N-2- hydroxy methyl acetate-methyl-propen amide, poly- N-2- ethoxy-methyl-propen amide, poly- N- hydroxyl first
Base-methyl-propen amide, poly- N- methoxy-methyl-propen amide, poly- N-3- methoxy-propyl-methyl-propen amide,
Poly- N- methyl-methyl-propen amide, poly- N- methyl-N-2,2- dimethoxy-ethyl-Methacrylamide, poly- N- morpholine-second
Base-Methacrylamide, poly- N-2,2,2- tri- chloro- 1- ethoxy-Methacrylamides, poly- hydroxy methylene, polyoxyethylene gather
Oxypropylene, polyvinyl alcohol, polyvinylpyrrolidone, poly- 2- ethyl -2- oxazoline, poly- 2- ethyl -2- oxazoline, poly- N- acetyl
Amino acryl amides, polypropylene oxygroup urea, hydroxyethyl cellulose, hydroxymethyl cellulose, ethylene polyethenoxy ether, polyethylene
Alcohol propylene ether.
5. the composition according to claim 4 applied to Capillary Electrophoresis, it is characterised in that: the denaturant includes
One or both of urea, formamide.
6. the composition according to claim 5 applied to Capillary Electrophoresis, it is characterised in that: the buffer includes
Two or more in Tris base, EDTA, TAPS.
7. the composition according to claim 6 applied to Capillary Electrophoresis, it is characterised in that: the composition PH is
6 to 10.
8. the composition according to claim 7 applied to Capillary Electrophoresis, it is characterised in that: the composition is used for
Capillary electrophoresis carries out the separation analysis of biomolecules mixture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811463721.0A CN109580756A (en) | 2018-12-03 | 2018-12-03 | Composition applied to Capillary Electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811463721.0A CN109580756A (en) | 2018-12-03 | 2018-12-03 | Composition applied to Capillary Electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109580756A true CN109580756A (en) | 2019-04-05 |
Family
ID=65926881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811463721.0A Pending CN109580756A (en) | 2018-12-03 | 2018-12-03 | Composition applied to Capillary Electrophoresis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109580756A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060108225A1 (en) * | 2004-11-19 | 2006-05-25 | Stephen Carson | High conductivity sieving matrices for high resolution biomolecule separations |
| CN1816573A (en) * | 2003-05-15 | 2006-08-09 | 艾普勒拉公司 | Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis |
| CN102220599A (en) * | 2010-07-01 | 2011-10-19 | 北京六合华大基因科技股份有限公司 | Quasi-interpenetrating polymer network used as capillary gel electrophoresis sieving medium and preparation method and use thereof |
| CN102276852A (en) * | 2011-05-05 | 2011-12-14 | 公安部物证鉴定中心 | Capillary tube dynamic coating polymer gel and preparation method thereof |
| CN102276853A (en) * | 2011-05-05 | 2011-12-14 | 公安部物证鉴定中心 | Blended copolymer gel with capillary self-coating effect and preparation method thereof |
| CN105579839A (en) * | 2013-09-26 | 2016-05-11 | 皮尔斯生物科技有限公司 | Electrophoretic matrix staining composition and method |
| WO2018217789A2 (en) * | 2017-05-22 | 2018-11-29 | Integenx Inc. | Compositions, methods, kits and devices for molecular analysis |
-
2018
- 2018-12-03 CN CN201811463721.0A patent/CN109580756A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1816573A (en) * | 2003-05-15 | 2006-08-09 | 艾普勒拉公司 | Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis |
| US20060108225A1 (en) * | 2004-11-19 | 2006-05-25 | Stephen Carson | High conductivity sieving matrices for high resolution biomolecule separations |
| CN102220599A (en) * | 2010-07-01 | 2011-10-19 | 北京六合华大基因科技股份有限公司 | Quasi-interpenetrating polymer network used as capillary gel electrophoresis sieving medium and preparation method and use thereof |
| CN102276852A (en) * | 2011-05-05 | 2011-12-14 | 公安部物证鉴定中心 | Capillary tube dynamic coating polymer gel and preparation method thereof |
| CN102276853A (en) * | 2011-05-05 | 2011-12-14 | 公安部物证鉴定中心 | Blended copolymer gel with capillary self-coating effect and preparation method thereof |
| CN105579839A (en) * | 2013-09-26 | 2016-05-11 | 皮尔斯生物科技有限公司 | Electrophoretic matrix staining composition and method |
| WO2018217789A2 (en) * | 2017-05-22 | 2018-11-29 | Integenx Inc. | Compositions, methods, kits and devices for molecular analysis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU712081B2 (en) | Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis | |
| CA1337953C (en) | Electrophoretic media | |
| US5883211A (en) | Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis | |
| Barril et al. | Introduction to agarose and polyacrylamide gel electrophoresis matrices with respect to their detection sensitivities | |
| JP2003523499A (en) | Copolymers for capillary gel electrophoresis | |
| Wang et al. | Evaluation of steric exclusion chromatography on cryogel column for the separation of serum proteins | |
| JP2815700B2 (en) | Chromatography column and separation method using the same | |
| WO2000042423A9 (en) | Copolymers for capillary gel electrophoresis | |
| CN109580756A (en) | Composition applied to Capillary Electrophoresis | |
| Doherty et al. | Sparsely cross‐linked “nanogels” for microchannel DNA sequencing | |
| CN100567339C (en) | (N-vinylamide class) polymkeric substance and multipolymer and their application in capillary electrophoresis | |
| CN102220599B (en) | Quasi-interpenetrating polymer network used as capillary gel electrophoresis sieving medium and preparation method and use thereof | |
| JP3997203B2 (en) | Electrophoresis buffer | |
| Zhou et al. | Electrophoretic separation of DNA using a new matrix in uncoated capillaries | |
| CN102940980B (en) | Preparation method and application for hydrophilic organic polymer liquid phase monolithic chromatographic column | |
| Yang et al. | Novel hydroxyethylcellulose‐graft‐poly acrylamide copolymer for separation of double‐stranded DNA fragments by CE | |
| CN114106350A (en) | Polyacrylamide chromatographic stationary phase and preparation method and application thereof | |
| US5219923A (en) | Electrophoretic media | |
| CN105367693B (en) | A kind of non-gel sieving matrices for Capillary Electrophoresis and preparation method thereof | |
| Gressel et al. | Temperature of acrylamide polymerization and electrophoretic mobilities of nucleic acids | |
| JPH05507351A (en) | Electrophoresis medium | |
| Zhou et al. | Graft copolymer composed of cationic backbone and bottle brush‐like side chains as a physically adsorbed coating for protein separation by capillary electrophoresis | |
| Wang et al. | A new quasi‐interpenetrating network formed by poly (N‐acryloyl‐tris‐(hydroxymethyl) aminomethane and polyvinylpyrrolidone: Separation matrix for double‐stranded DNA and single‐stranded DNA fragments by capillary electrophoresis with UV detection | |
| WO2020086366A1 (en) | Methods for separating large nucleic acids under denatured conditions | |
| Okada et al. | Channel wall coating on a poly‐(methyl methacrylate) CE microchip by thermal immobilization of a cellulose derivative for size‐based protein separation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190529 Address after: 628000 No. 3 Xi'an Street, Lizhou District, Guangyuan City, Sichuan Province Applicant after: Yang Cuifang Applicant after: Chengdu New Genetic Biotechnology Co., Ltd. Address before: 628000 No. 3 Xi'an Street, Lizhou District, Guangyuan City, Sichuan Province Applicant before: Yang Cuifang |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190405 |