CN109566647B - Fosthiazate composition containing neonicotinoid insecticide components - Google Patents

Abstract

The invention belongs to the field of pesticides, and particularly relates to a fosthiazate composition containing neonicotinoid insecticidal components. The active ingredients are as follows: neonicotinoid insecticide component and fosthiazate. The mass ratio of the fosthiazate to the neonicotinoid insecticide components can be as follows: 100:1-100:50. According to the invention, the compounding of the furanonicotine and the fosthiazate is researched, the compounding of the neonicotinoid insecticidal component and the fosthiazate shows a good activity effect, and the compounding of the furanonicotine and the fosthiazate can effectively alleviate the increasingly serious resistance problem of the fosthiazate.

Description

Fosthiazate composition containing neonicotinoid insecticide components

Technical Field

The invention belongs to the field of pesticides, and particularly relates to a fosthiazate composition containing neonicotinoid insecticidal components.

Background

The novel nicotine (chloronicotine and nicotine) is mainly combined with acetylcholinesterase receptors, and shows toxic effect on insects by inhibiting the activity of membrane nicotinic acetylcholine receptors after synapse of nerve cells. In contrast to other nerve agents acting on acetylcholine receptors, neonicotinoids have both cationic and anionic binding sites within the molecule that bind to acetylcholine esterase receptors.

Conventionally, a novel nicotine is required to have a nitrogen-containing aromatic ring corresponding to a pyridine ring of nicotine, an anionic structure of chloronicotine is a chloropyridine ring, and an anionic structure of thionicotine is a chlorothiopyridine ring. Furanotine is greatly changed in structure compared with chloronicotinoids (imidacloprid, thiacloprid, acetamiprid and nitenpyram) and thiocatniine (clothianidin and thiamethoxam). Most notably, the anionic site is changed from a chloropyridine ring to furan, an oxygen-containing five-membered heterocyclic compound.

Furanonicotinoids, which do not contain a chlorine atom and an aromatic ring, while still being classified as novel insecticides, have been completely altered in their chemical structure compared to nicotine.

At low doses, neonicotinoid insecticides are not lethal to larger organisms including mammals, birds and even bees, but can severely disrupt the ability of bees to navigate, feed, reproduce and form new bee colonies. In the beginning of 5 months in 2018, voting is carried out in the European Union, and three neonicotinoid insecticides of thiamethoxam, imidacloprid and clothianidin are forbidden due to harm to bees.

Fosthiazate (fosthiazate) is an organic phosphorus nematicide, has the main action mode of inhibiting the synthesis of acetylcholinesterase of root-knot nematodes, and can be used for preventing and treating various root-knot nematodes, aphids and the like. Since the last 50 s, the pesticide composition is widely applied to the root-knot nematode control of vegetables and fruit trees. Due to the long history of use of organophosphorus insecticides, the drug resistance of target pests is becoming more and more serious, and more than 70% of organophosphorus pesticides are forbidden after 21 st century. In 1996, the taiwan area has found that root knot nematodes showed a high resistance to fosthiazate. In order to increase crop yield, farmers often adopt larger-dose pesticide preparations to achieve the purpose of prevention and treatment, which is accompanied by the problems of overproof pesticide residues on agricultural products, environmental threats and the like.

The problems that the drug resistance of target pests to pesticides is reduced and the application amount of the pesticides is reduced become a problem to be solved urgently in the whole industry.

At present, no report related to compounding and application of furanonicotine and fosthiazate is available.

Disclosure of Invention

The invention aims to provide a fosthiazate composition containing neonicotinoid insecticide ingredients.

The invention provides a fosthiazate composition containing neonicotinoid insecticidal components, which comprises the following active components: neonicotinoid insecticide component and fosthiazate.

The neonicotinoid insecticidal components can be as follows: a furanone nicotine compound.

The furanonicotinoid compound may be: one or more of dinotefuran, flupirfuranone and other furan nicotine compounds.

In the insecticidal composition, the mass ratio of fosthiazate to neonicotinoid insecticidal components can be as follows: 100:1-100:50, specifically 100:5-100:50, 100:10-100:50, 100:20-100:50, 100:5, 100:10, 100:20 or 100: 50.

The composition can be added with a surfactant, a carrier, an auxiliary material and the like to prepare various formulations such as aqueous solution, aqueous emulsion, wettable powder, soluble granules, water dispersible granules, suspending agent, oil suspending agent or effervescent granules and the like besides the active ingredients.

Preferably, wettable powders, suspensions, etc. are prepared.

The preparation method comprises preparing into desired dosage forms by conventional method.

The content of neonicotinoid insecticidal components and fosthiazate in the preparation is 10-30% by mass of pure raw medicines.

The application of the neonicotinoid insecticidal ingredients in enhancing the performance of the fosthiazate also belongs to the protection scope of the invention.

The property may specifically be a root-knot nematode killing property.

The invention also provides a method for killing root-knot nematodes by using the composition.

The method for killing root-knot nematodes by using the composition comprises the following steps: spraying the dug seedling holes with the liquid medicine prepared from the composition before transplanting the plants.

The application of the neonicotinoid insecticidal component in retarding the resistance of the root-knot nematode to the fosthiazate also belongs to the protection scope of the invention.

According to the invention, the compounding of the furanonicotine and the fosthiazate is researched, the compounding of the neonicotinoid insecticidal component and the fosthiazate shows a good activity effect, and the compounding of the furanonicotine and the fosthiazate can effectively alleviate the increasingly serious resistance problem of the fosthiazate.

Detailed Description

The present invention will be described below with reference to specific examples, but the present invention is not limited thereto.

The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, materials and the like used in the following examples are commercially available unless otherwise specified.

Example 1 Thiazolophosphine dinotefuran wettable powder

10% of fosthiazate, 2% of dinotefuran, 5% of calcium lignosulphonate, 2.6% of sodium dodecyl sulfate, 2.4% of white carbon black, 2.3% of phenyl phosphite, 33% of kaolin and 42.7% of diatomite, and the thidiazuron and dinotefuran wettable powder with the effective component of 12% is prepared after crushing and mixing.

Example 2 Thiazolephosphine Fluopyranone suspension

10% of fosthiazate, 2% of fluo-furazone, 1% of furfural, 3.6% of polyoxy alkyl phenyl ether phosphate, 12% of propylene glycol, 0.2% of xanthine, 0.1% of polyalkylpolysiloxane and 71.1% of water, and the mixture is subjected to wet grinding until the average particle size reaches 3 +/-0.5 mu m of the volume average particle size to obtain the fosthiazate-fluo-furazone suspending agent with the active ingredient of 12%.

Examples 3,

Test time: no. 3/10 in 2017-16/7 in 2017

Test site: in a sunlight greenhouse in a basement of a vegetable institute in Anyang city, the last crop is cucumber, and root-knot nematodes are serious.

Test protocol: the fosthiazate and the dinotefuran are compounded according to a certain proportion to prepare seven granules with 10 percent of fosthiazate or fosthiazate and dinotefuran with the total effective content of 10 percent, wherein the effective component ratios are 100:0, 100:1, 100:2, 100:5, 100:10, 100:20, 100:50 and the like, and each proportion is repeated twice and is applied according to 1000 grams of mu.

Test procedure

Transplanting is carried out during the period of 2-3 leaves and 1 heart of tomato seedlings. Before transplanting, spraying the prepared liquid medicine to the dug seedling holes by using a sprayer. Observing whether the effect of the medicament on the seedling recovery of the tomato seedlings is influenced within 1-2 weeks after transplanting. After topping, 20 tomatoes were dug for each treatment, disease occurrence was investigated and disease index was calculated after washing the roots, with disease grading criteria as follows:

grading standard of tomato root-knot nematode:

level 0: no disease occurs;

level 1: root nodules occur in less than 5 percent of root systems;

and 3, level: 6 to 25 percent of root systems have root nodules;

and 5, stage: 26% -50% of root systems have root nodules;

and 7, stage: 51% -75% of root systems have root nodules;

and 9, stage: more than 75% of root systems have root nodules until the plant dies.

Test results

Through investigation, the disease indexes and the prevention and treatment effects of different treatments are shown in the following table:

the annual nematode in the plot is seriously damaged, and the fosthiazate is not used for preventing and controlling, so that the fosthiazate insecticidal effect of 100:0 basically meets the requirement. As can be seen from the data in the table, after the dinotefuran is added, the control effect of the fosthiazate is obviously improved compared with that of the fosthiazate single agent.

Example 4

Test time: 13/1/2018

Test crops and control subjects:

the greenhouse cucumber is used for preventing and controlling cucumber root-knot nematodes.

Test environment and facility cultivation conditions:

the experimental field is arranged in a winter-warm sunlight greenhouse of a vegetable research institute in the New countryside city. The test field has flat vigor, fertile soil, convenient irrigation and consistent cultivation management. The row spacing of cucumber is 40-45cm, and the plant spacing is 35-40 cm. In 2017, cucumber planted in the plot was subjected to a root-knot nematode control test by fosthiazate.

Test protocol:

the fosthiazate and the fluopyram are compounded according to a certain proportion to prepare a microcapsule suspending agent with the total active ingredients of 10 percent of seven total active ingredients, such as 100:0, 100:1, 100:2, 100:5, 100:10, 100:20, 100:50 and the like, and the microcapsule suspending agent is applied by 1000 milliliters per mu. One application per group, blank 1 group, for a total of 8 treatments. When the cucumber seedlings are transplanted, the agents are mixed with water according to the designed dosage, transplanting holes are sprayed by a conventional electric sprayer, and 4L of spraying liquid medicine (267L per mu) is sprayed in each district. The sprayer is cleaned when dispensing or replacing the liquid medicine.

The investigation method comprises the following steps:

sampling is carried out at 3 random points in parallel, 10 plants are investigated at each point, and the incidence and disease index are investigated at each treatment of 30 plants, and are shown in a grading standard table.

Table 1: grading standard table

Disease index ∑ (number of diseased plants at each stage × disease-grade value)/(total number of investigated plants × highest-grade value) × 100

Safety investigation:

and 3-7 days after the pesticide is applied, comparing with a blank control, checking whether the pesticide has phytotoxicity on the crops, and recording the type and the degree of the phytotoxicity. Whether the growth period is advanced or delayed is observed. In addition, beneficial effects on the crop were noted.

The analysis method comprises the following steps:

the control effect is calculated according to a conventional method, and statistical analysis is carried out by a duncan new complex pole difference method.

Results and analysis:

incidence of disease

Table 2: incidence analysis

As can be seen from the table 2, when no fluopyram is mixed, the control effect of the cucumber root-knot nematode disease rate is not ideal when the dosage of the 10% fosthiazate microcapsule suspending agent preparation is 1000 ml/mu, the cucumber root-knot nematode disease rate is already high according to the nematode because the fosthiazate is used for a long time in the plot, the control effect is obviously improved after the fluopyram is added, and when the ratio of the fosthiazate to the fluopyram reaches 100:50, the very obvious killing effect is shown.

Disease index:

table 3: index of disease analysis

As can be seen from Table 3, the control effect of the composite of fosthiazate and fluopyram is obviously improved compared with that of a fosthiazate single agent after the composite of fosthiazate and fluopyram in a series of proportions, wherein the ratio of the fosthiazate to the fluopyram is 100: the control effect of 1 is improved by 113% compared with that of a fosthiazate single agent, the control effect of 100:2 is slightly reduced compared with that of 100:1, but is also obviously higher than that of the fosthiazate single agent, the control effects of 100:5, 100:10, 100:20 and 100:50 are all more than 60%, wherein when the fosthiazate and the fluofuranone are compounded at a ratio of 100:50, the control effect is as high as 96.97%, and the analysis shows that after the fluofuranone is added, the control effect of the fosthiazate on the root-knot nematodes is obviously enhanced, and the resistance of the root-knot nematodes on the fosthiazate is effectively overcome.

Claims (3)

1. A method for killing cucumber root-knot nematodes by using a composition, comprising: spraying the dug seedling holes with the liquid medicine prepared by the composition before transplanting the plants;

the active ingredients of the composition are as follows: neonicotinoid insecticidal ingredients and fosthiazate;

the neonicotinoid insecticide component is fluroxypyr;

in the composition, the mass ratio of fosthiazate to fluropyranone is as follows: 100: 50;

the cucumber root-knot nematode is a cucumber root-knot nematode which has higher drug resistance to fosthiazate.

2. The method of claim 1, wherein: the composition not only contains active ingredients of neonicotinoid insecticidal ingredients and fosthiazate, but also can be prepared into various dosage forms by adding auxiliary materials.

3. The method of claim 2, wherein: the preparation is water agent, aqueous emulsion, wettable powder, soluble granules, water dispersible granules, suspending agent, oil suspending agent, tablets or effervescent granules;

the content of neonicotinoid insecticidal components and fosthiazate in the preparation is 10-30% by mass of pure raw medicines.