CN109557163A - A kind of Living single cell situ extracting and on-line mass spectroscopy detection device and application - Google Patents
A kind of Living single cell situ extracting and on-line mass spectroscopy detection device and application Download PDFInfo
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Abstract
The present invention provides a kind of Living single cell situ extracting and on-line mass spectroscopy detection device and application, described device includes: microfluidic platform, for extracting fluid to unicellular probe transmission；Cell operation platform, culture has cell to be detected thereon；Unicellular probe is located on cell operation platform, to be detected unicellular for target to be isolated, and realizes that unicellular extraction obtains unicellular extract；Online ionization apparatus, for receiving unicellular extract and unicellular extract being formed ionizing spray online；Detection device, for receiving the ionizing spray of online ionization apparatus formation and being detected to it.When carrying out cell detection using the device, after the cell detection of extraction to its marker, maximizing retains intracellular albumen and information nucleic acid for subsequent analysis.
Description
Technical field
The invention belongs to unicellular Mass Spectra technical field, it is related to a kind of Living single cell situ extracting and on-line mass spectroscopy
Detection device and its application.
Background technique
Single cell analysis technology is incorporated in by the technologies such as microflow control technique and electrochemical analysis, fluorescence analysis, mass spectrum
Experimental cell marker, metabolin, genome and protein groups etc. are analyzed on individual cell level.With the progress of analysis means,
More and more Single cell analysis technologies are come into being.Since single cell analysis can be surveyed for medical diagnosis on disease, gene to low consumption
Sequence provides important information and is widely noticed.
Important means of the mass spectrum as unicellular chemical composition analysis has highly sensitive, high-throughput and analyzes simultaneously
The characteristics of a variety of important components.Presently, there are and widely used unicellular mass-spectrometric technique in, unicellular drop technique is due to list
Cell contains rate bottom, and experimental error is difficult to avoid that；The analysis to cellular content may be implemented in unicellular probe technique, but every
Secondary piercing position can not determine, can not embody entire single celled all information.Therefore, developing one kind may be implemented former detection
Unicellular mass-spectrometric technique will to this field have great impetus.
Summary of the invention
In order to solve the problems, such as existing unicellular mass-spectrometric technique, it is former that the present invention provides a kind of Living single cells
Position extraction and on-line mass spectroscopy detection device, when carrying out cell detection using the device, in the cell detection by extraction to its mark
After will object, maximizing retains intracellular albumen and information nucleic acid for subsequent analysis.
One aspect of the present invention provides a kind of Living single cell situ extracting and on-line mass spectroscopy detection device, the device packet
It includes:
Microfluidic platform, for extracting fluid to unicellular probe transmission；
Cell operation platform, culture has cell to be detected thereon；
Unicellular probe is located on cell operation platform, to be detected unicellular for target to be isolated, and realizes slender
Born of the same parents, which extract, obtains unicellular extract；
Online ionization apparatus, for receiving unicellular extract and unicellular extract being formed to ionization spray online
Mist；
Detection device, for receiving the ionizing spray of online ionization apparatus formation and being detected to it.
According to the preferred embodiment of the present invention, the unicellular probe includes setting gradually along extraction fluid flow direction
Fluid inlet, unicellular extraction cell and fluid outlet, fluid inlet and fluid outlet be connected with unicellular extraction cell.Extraction
Fluid enters unicellular extraction cell from fluid inlet and then flows out from fluid outlet.
According to the preferred embodiment of the present invention, the unicellular extraction cell is constructed to be permeable to accommodate individual cells, it is preferable that
Unicellular extraction cell is configured to be only capable of accommodating individual cells.
According to the preferred embodiment of the present invention, the cell operation platform includes:
Objective table；
Cell culture apparatus is located on the objective table, cell culture fluid is loaded in the cell culture apparatus, uses
In culture cell to be detected；
The alignment device being set to below the objective table, for unicellular being observed and being positioned to target is to be extracted.
According to the preferred embodiment of the present invention, cell is planted in cell training with the density of 100-10000/square centimeter
It supports in device.
In certain embodiments of the present invention, the cell culture apparatus is culture dish, is placed on objective table.
According to the preferred embodiment of the present invention, the alignment device is micro objective, and the cell in culture dish can be with
It is observed by micro objective, can accurately confirm whether unicellular probe is directed at individual cells.
According to the preferred embodiment of the present invention, the microfluidic platform includes:
Fluid circuit comprising the first fluid pipeline that is connected with the fluid inlet and be connected with the fluid outlet
Second fluid pipeline；
Wriggling syringe pump, for driving the extraction fluid in fluid circuit.
Wriggling syringe pump driving extraction fluid enters first fluid pipeline, the stream by first fluid pipeline through unicellular probe
Body entrance enters unicellular probe, then flows through unicellular extraction cell and extracts to unicellular, unicellular extract is through fluid
Outlet flows into second fluid pipeline and enters online ionization apparatus.
According to the preferred embodiment of the present invention, the wriggling syringe pump is with 0.2-1 μ L/min, preferably with 0.5 μ L/min
Speed driving fluid circuit in extraction fluid, realize continuous flow extraction.
According to the preferred embodiment of the present invention, the first fluid pipeline is identical with the internal diameter of second fluid pipeline, excellent
Selection of land, internal diameter are 20-100 μm, preferably 50 μm.
According to the preferred embodiment of the present invention, the online ionization apparatus includes power supply, needle like extremity and metal two
Port valve, for the metal two-port valve for receiving unicellular extract and being connected with needle like extremity, needle like extremity provides ionization
Spraying, power supply connects metal two-port valve.
According to the preferred embodiment of the present invention, the power supply uses commercial high voltage power supply, and voltage 2000-4500V is used
Spraying required voltage is formed to provide needle like extremity.
Preferably, the detection device includes detection device injection port and detection device main body, the needle like extremity face
Detection device injection port is detected so that being directly entered detection device after needle like extremity formation ionizing spray.
According to the preferred embodiment of the present invention, the unicellular probe is prepared using soft lithography.Preferably, described
Unicellular probe is used to be made with elastic and good translucency plastic material, is preferably made by dimethyl silicone polymer.
According to another aspect of the present invention, above-mentioned apparatus is provided in unicellular Mass Spectrometer Method and unicellular drug screening
Application.
A kind of Living single cell situ extracting and on-line mass spectroscopy detection method comprising:
Step S1, Living single cell situ extracting recited above and on-line mass spectroscopy detection device are provided；
Step S2, cell to be detected is cultivated on cell operation platform；
Step S3, wait for target vertically close to cell operation platform by adjusting cell operation platform unicellular probe
Detection cell is placed in below unicellular probe, and adjustment cell operation platform is bonded unicellular probe with cell operation platform, from
And it is to be detected unicellular that target is isolated；
Step S4, extraction fluid is transmitted to unicellular probe by microfluidic platform, and flows into online ionization apparatus,
Ionizing spray is formed through online ionization apparatus, and is detected into detection device.
According to the preferred embodiment of the present invention, the extraction fluid is methanol.Methanol has cell fixed function, cell
By fixation in situ after extraction, a large amount of molecular informations not detected can be retained for subsequent analysis.
When described device is applied to unicellular drug screening, extraction fluid is replaced with into the culture medium solution containing drug,
Cell can be monitored in real time to the metaboilic level of drug, carry out unicellular drug screening.
Beneficial effects of the present invention:
1, the present invention collects the continuous flow control of microfluid, unicellular situ extracting, ionizes and be detected in real time one online,
Targeting extraction in situ can be carried out to the attached cell cultivated in culture dish, and realizes Mass Spectrometer Method in situ, with realizing micro-damage
To single celled analysis of markers in situ.
2, unicellular probe of the invention can be sampled one by one to unicellular, and can be maximum after sampling analysis
The surplus materials ingredient and content of degree retained under cell adherent growth state, can be used for other analyses.
3, cell analysis speed of the invention is fast, can complete the sampling analysis of individual cells in a short period of time.
4, cell extraction liquid can be replaced with the culture medium solution containing drug by the present invention, and cell pair can be monitored in real time
The metaboilic level of drug carries out unicellular drug screening.
Detailed description of the invention
Fig. 1 is the Living single cell situ extracting and on-line mass spectroscopy detection device of the embodiment of the present invention；
Fig. 2 is the structural schematic diagram of the unicellular probe of the embodiment of the present invention；
Wherein, 1 wriggling syringe pump is represented, 2-1 represents first fluid pipeline, and 2-2 represents second fluid pipeline, and 3 represent training
Ware is supported, 4 represent micro objective, and 5 tables represent unicellular probe, and 6 represent cell, and 7 represent metal two-port valve, and 8 represent power supply, and 9
Needle like extremity is represented, 10 represent the injection port of detection device, and 11 represent fluid inlet, and 12 represent unicellular extraction cell, and 13 represent
Fluid outlet.
Specific embodiment
The present invention is described in detail with reference to embodiments, but the present invention is not limited by following embodiments.
Embodiment 1
As depicted in figs. 1 and 2, a kind of Living single cell situ extracting and on-line mass spectroscopy detection device comprising:
Microfluidic platform comprising wriggling syringe pump 1 and fluid circuit, fluid circuit include first fluid pipeline 2-1 and
Second fluid pipeline 2-2, wriggling syringe pump are used to drive the extraction fluid in fluid circuit, extraction fluid are transmitted to unicellular
Probe；
Cell operation platform comprising objective table (not shown in figure 1), the culture dish 3 being placed on objective table, culture dish 3
In be loaded with cell culture fluid, culture has cell to be detected in culture dish, micro objective 4 is provided with below objective table,
It is used to that cell to be detected to be observed and be positioned；
Unicellular probe above cell operation platform comprising enter along the fluid that fluid flow direction is set gradually
Mouth 11, unicellular extraction cell 12 and fluid outlet 13, fluid inlet and fluid outlet are connected with unicellular extraction cell.It is micro-fluidic
The first fluid pipeline 2-1 of platform is connected with fluid inlet 11, and fluid outlet 13 is connected with second fluid pipeline 2-2；
Online ionization apparatus comprising power supply 8, needle like extremity 9 and metal two-port valve 7, metal two-port valve 7 and needle-shaped end
End 9 is connected, and metal two-port valve 7 receives the unicellular extract from second fluid pipeline 2-2 and passes to needle like extremity 9,
Needle like extremity 9 is formed by spraying, and power supply 8 is connected with metal two-port valve 7, forms spraying required voltage for providing needle like extremity；
Detection device comprising detection device injection port 10 and detection device main body (not shown in figure 1), detection device into
Sample mouth 10 and 9 face of needle like extremity, so that the spraying detection device that enters that needle like extremity is formed is detected.
A kind of Living single cell situ extracting and on-line mass spectroscopy detection method comprising:
Living single cell situ extracting recited above and on-line mass spectroscopy detection device are provided,
Cell is planted in culture dish 3 with the density of 100-10000/square centimeter, unicellular probe 5 is vertically leaned on
Target cell 6 to be detected, the underface of unicellular probe, the cell in culture dish 3 are adjusted to by objective table by nearly culture dish 3
6 can be observed by micro objective 4, so that it is determined that whether unicellular probe is targeted by individual cells.Make by adjusting objective table
The bottom surface that unicellular probe 5 is close to culture dish 3 is obtained, after unicellular probe is bonded with the bottom surface of culture dish, cell 6 can be closed
In unicellular probe, so that target cell 6 to be detected and the culture medium solution of surrounding and other cells be kept apart.It will extraction
Fluid is taken to inject fluid circuit 2-1 through wriggling syringe pump 1, extraction fluid enters unicellular extraction cell 12 through fluid inlet 11,
Single celled extraction is realized in unicellular extraction cell 12, unicellular extract liquor flows out unicellular probe 5 through fluid outlet 13, through
Two fluid circuit 2-2 and metal two-port valve 7 are transmitted to needle like extremity 9, and high-voltage potential is conducted by solution to needle like extremity 9,
Needle like extremity 9 is persistently formed by spraying, and is entered detection device through detection device injection port and carried out on-line checking.
The cell 6 in fluid continuous action and culture dish 3 is extracted, by the regular hour, the marker on cell 6 is extracted
It takes into the formation continuous ionicization of needle like extremity 9 and is sprayed, cell 6 extracted is fixed on culture dish 3, can be used for subsequent
Other analysis.
It should be noted that embodiment described above for explaining only the invention, is not constituted to of the invention any
Limitation.By referring to exemplary embodiments, invention has been described, it should be appreciated that word used in it is descriptive
With explanatory vocabulary, rather than limited vocabulary.The present invention can be made within the scope of the claims by regulation
Modification, and the present invention is revised in without departing substantially from scope and spirit of the present invention.Although the present invention described in it relates to
And specific method, material and embodiment, it is not intended that the present invention is limited to particular case disclosed in it, on the contrary, this hair
It is bright to can be extended to other all methods and applications with the same function.
Claims (10)
1. a kind of Living single cell situ extracting and on-line mass spectroscopy detection device, comprising:
Microfluidic platform, for extracting fluid to unicellular probe transmission；
Cell operation platform, culture has cell to be detected thereon；
Unicellular probe is located on cell operation platform, to be detected unicellular for target to be isolated, and realizes unicellular extraction
It takes and obtains unicellular extract；
Online ionization apparatus, for receiving unicellular extract and unicellular extract being formed ionizing spray online；
Detection device, for receiving the ionizing spray of online ionization apparatus formation and being detected to it.
2. the apparatus according to claim 1, which is characterized in that the unicellular probe includes along extraction fluid flow direction
Fluid inlet, unicellular extraction cell and fluid outlet, fluid inlet and the fluid outlet set gradually and unicellular extraction cell phase
Connection.
3. the apparatus of claim 2, which is characterized in that the unicellular extraction cell is constructed to be permeable to accommodate single thin
Born of the same parents, it is preferable that unicellular extraction cell is configured to be only capable of accommodating individual cells.
4. device according to any one of claim 1-3, which is characterized in that the cell operation platform includes:
Objective table；
Cell culture apparatus is located on the objective table, cell culture fluid is loaded in the cell culture apparatus, for training
Support cell to be detected；
The alignment device being set to below the objective table, for target it is to be detected it is unicellular observed and positioned, preferably
The alignment device is micro objective.
5. the device according to any one of claim 2-4, which is characterized in that the microfluidic platform includes:
Fluid circuit comprising the first fluid pipeline being connected with the fluid inlet and be connected with the fluid outlet second
Fluid circuit；
Wriggling syringe pump, for driving the extraction fluid in fluid circuit.
6. device according to any one of claims 1-5, which is characterized in that the online ionization apparatus includes electricity
Source, needle like extremity and metal two-port valve, the metal two-port valve are used to receive unicellular extract and be connected with needle like extremity,
Power supply connects metal two-port valve.
7. device according to claim 1 to 6, which is characterized in that the unicellular probe uses soft lithographic skill
Art preparation, is preferably made by dimethyl silicone polymer.
8. application of the device of any of claims 1-7 in unicellular Mass Spectrometer Method and unicellular drug screening.
9. a kind of Living single cell situ extracting and on-line mass spectroscopy detection method comprising:
Step S1, device of any of claims 1-7 is provided；
Step S2, cell to be detected is cultivated on cell operation platform；
Step S3, keep target to be detected by adjusting cell operation platform vertically close to cell operation platform unicellular probe
Cell is placed in below unicellular probe, and then adjusting cell operation platform is bonded unicellular probe with cell operation platform, from
And it is to be detected unicellular that target is isolated；
Step S4, extraction fluid by microfluidic platform is transmitted to unicellular probe, and flows into online ionization apparatus, through
Line ionization apparatus forms ionizing spray, is detected into detection device.
10. according to the method described in claim 9, it is characterized in that, the extraction fluid is methanol.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110988102A (en) * | 2019-12-10 | 2020-04-10 | 北京工业大学 | Visual sheath-flow-free single-cell mass spectrometry system |
| CN111199863A (en) * | 2020-01-10 | 2020-05-26 | 中国科学院深圳先进技术研究院 | Sampling system, mass spectrometry device, sampling method and mass spectrometry method |
| CN111808750A (en) * | 2020-07-15 | 2020-10-23 | 清华大学 | Micro-fluidic chip device, in-situ detection method for living unicells and application |
| CN112067534A (en) * | 2020-09-26 | 2020-12-11 | 宁波大学 | Single cell mass spectrometry system and method |
| CN112378987A (en) * | 2021-01-18 | 2021-02-19 | 宁波华仪宁创智能科技有限公司 | Mass spectrometry system with cleaning function and method |
| CN112444583A (en) * | 2021-02-01 | 2021-03-05 | 宁波大学 | Cell detection device and method based on paper-based discharge technology |
| CN117368073A (en) * | 2023-09-04 | 2024-01-09 | 中山大学·深圳 | Multi-mode liquid drop detection system and method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120164717A1 (en) * | 2007-07-18 | 2012-06-28 | Joseph Irudayaraj | Identity profiling of cell surface markers |
| CN104392886A (en) * | 2014-09-30 | 2015-03-04 | 浙江大学 | Capillary needle, and electro-spray ionization mass spectrometry analytical apparatus and method |
| CN107446820A (en) * | 2017-09-28 | 2017-12-08 | 清华大学 | Unicellular sampling and in situ detection mass spectrometer interface device based on micro-fluidic chip |
| CN107863286A (en) * | 2017-11-06 | 2018-03-30 | 清华大学 | A kind of Living single cell lysisin situ and online ionization detection mass spectrometer interface device |
| CN108593755A (en) * | 2018-04-28 | 2018-09-28 | 清华大学 | A kind of extracellular secretion matter sampling and in-situ detection method and device |
| CN209858475U (en) * | 2018-11-27 | 2019-12-27 | 清华大学 | In-situ extraction and online mass spectrum detection device for living unicells |
-
2018
- 2018-11-27 CN CN201811424901.8A patent/CN109557163B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120164717A1 (en) * | 2007-07-18 | 2012-06-28 | Joseph Irudayaraj | Identity profiling of cell surface markers |
| CN104392886A (en) * | 2014-09-30 | 2015-03-04 | 浙江大学 | Capillary needle, and electro-spray ionization mass spectrometry analytical apparatus and method |
| CN107446820A (en) * | 2017-09-28 | 2017-12-08 | 清华大学 | Unicellular sampling and in situ detection mass spectrometer interface device based on micro-fluidic chip |
| CN107863286A (en) * | 2017-11-06 | 2018-03-30 | 清华大学 | A kind of Living single cell lysisin situ and online ionization detection mass spectrometer interface device |
| CN108593755A (en) * | 2018-04-28 | 2018-09-28 | 清华大学 | A kind of extracellular secretion matter sampling and in-situ detection method and device |
| CN209858475U (en) * | 2018-11-27 | 2019-12-27 | 清华大学 | In-situ extraction and online mass spectrum detection device for living unicells |
Non-Patent Citations (2)
| Title |
|---|
| 李海芳: "基于微流控芯片-质谱联用的细胞分析研究进展", 中国科学：化学, vol. 44, no. 5, pages 777 - 783 * |
| 毛思锋: "微流控芯片在细胞药物代谢和细胞信号传导研究中的应用", 中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑, no. 07, pages 016 - 178 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110988102A (en) * | 2019-12-10 | 2020-04-10 | 北京工业大学 | Visual sheath-flow-free single-cell mass spectrometry system |
| CN111199863A (en) * | 2020-01-10 | 2020-05-26 | 中国科学院深圳先进技术研究院 | Sampling system, mass spectrometry device, sampling method and mass spectrometry method |
| WO2021139412A1 (en) * | 2020-01-10 | 2021-07-15 | 中国科学院深圳先进技术研究院 | Sampling system, mass spectrometry analysis device, sampling method, and mass spectrometry analysis method |
| CN111199863B (en) * | 2020-01-10 | 2021-07-16 | 中国科学院深圳先进技术研究院 | Sampling system, mass spectrometry device, sampling method and mass spectrometry method |
| CN111808750A (en) * | 2020-07-15 | 2020-10-23 | 清华大学 | Micro-fluidic chip device, in-situ detection method for living unicells and application |
| CN112067534A (en) * | 2020-09-26 | 2020-12-11 | 宁波大学 | Single cell mass spectrometry system and method |
| CN112378987A (en) * | 2021-01-18 | 2021-02-19 | 宁波华仪宁创智能科技有限公司 | Mass spectrometry system with cleaning function and method |
| CN112378987B (en) * | 2021-01-18 | 2021-05-07 | 宁波华仪宁创智能科技有限公司 | Mass spectrometry system with cleaning function and method |
| CN112444583A (en) * | 2021-02-01 | 2021-03-05 | 宁波大学 | Cell detection device and method based on paper-based discharge technology |
| CN112444583B (en) * | 2021-02-01 | 2021-05-07 | 宁波大学 | Cell detection device and method based on paper-based discharge technology |
| CN117368073A (en) * | 2023-09-04 | 2024-01-09 | 中山大学·深圳 | Multi-mode liquid drop detection system and method |
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