CN109549975A - A kind of preparation method of Chinese medical extract and its Chinese medical extract of preparation - Google Patents
A kind of preparation method of Chinese medical extract and its Chinese medical extract of preparation Download PDFInfo
- Publication number
- CN109549975A CN109549975A CN201811541786.2A CN201811541786A CN109549975A CN 109549975 A CN109549975 A CN 109549975A CN 201811541786 A CN201811541786 A CN 201811541786A CN 109549975 A CN109549975 A CN 109549975A
- Authority
- CN
- China
- Prior art keywords
- fruit
- preparation
- weight
- parts
- concentrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/37—Celastraceae (Staff-tree or Bittersweet family), e.g. tripterygium or spindletree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/638—Ligustrum, e.g. Chinese privet
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/708—Rheum (rhubarb)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention belongs to pharmaceutical technology fields, and in particular to a kind of preparation method for treating diabetic nephropathy (DN) Chinese medical extract and its Chinese medical extract, the purposes of preparation etc..The bulk pharmaceutical chemicals of Chinese medical extract of the present invention form are as follows: Radix Astragali, Gorgon fruit, the fruit of Cherokee rose, Common Achyranthes Herb, the fruit of glossy privet, Ramulus euonymi and rheum officinale；The preparation method includes: (1) Radix Astragali, fruit of glossy privet, kowtows grass using alcohol extracting；(2) Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale are mentioned using water.It is proved through animal pharmacodynamics test, the Chinese medical extract obtained compared with prior art using preparation method provided by the invention has better drug effect.
Description
Technical field
The invention belongs to pharmaceutical technology fields, and in particular to a kind of preparation method of Chinese medical extract and its Chinese medicine of preparation
Extract.
Background technique
Diabetic nephropathy (diabetic nephropathy, DN) is clinical common and multiple diabetic complication,
It is one of the complication of diabetes most serious.Diabetic nephropathy is the main microvascular complication of diabetes, refers mainly to diabetes
Property glomerulosclerosis, a kind of Glomerular lesions based on vascular lesion.How asymptomatic early stage is, and blood pressure can be normal or higher,
Its incidence increases as the course of disease of diabetes extends.Diabetes early stage kidney volume increases, and glomerular filtration rate increases, in height
Filtration state gradually appears discontinuity albuminuria or microalbuminuria later, with the course of disease extension occur continue albuminuria,
Oedema, hypertension, glomerular filtration rate reduce, and then renal insufficiency, uremia.It is reported that the disease incidence of DN is in 1 type glycosuria
It is about 40~50% in disease, DN incidence is about 30~40% in diabetes B.Diabetic nephropathy has been End-stage renal disease
(ESRF) most important reason and one of the main reason for lethal diabetic in, has carried out this large amount of both at home and abroad
Research, but pathogenesis not yet illustrates completely so far, there is no effective drug that it is prevented to occur and deteriorate.
Patent document CN102228536A discloses a kind of Chinese medical extract for treating diabetic nephropathy, composition are as follows:
600~1000 parts of Radix Astragali, 400~600 parts of Gorgon fruit, 400~600 parts of the fruit of Cherokee rose, 600~1000 parts of Common Achyranthes Herb, the fruit of glossy privet 400~
480 parts, 400~480 parts of Ramulus euonymi and 320~480 parts of rheum officinale.The document is mentioned, and clinic uses the diabetogenous nephrosis of international uniform
Sick (DN) diagnostic criteria passes through 300 according to random controls principle by the Chinese native medicine compound prescription pellet of the invention and Lotensin controlled observation
Example Diabetic Nephropathy patients observation, the results showed that the 1. compound granular can improve DN patient clinical symptom；2. can obviously reduce morning
Phase DN urinary albumin and clinical period diabetic nephropathy Urine proteins, protect renal function, to effectively prevent the progress of early stage DN.Root
According to the standard of curative effect evaluation of formulation, the Chinese native medicine compound prescription pellet group total effective rate 86.7% of the invention, hence it is evident that be better than control group.Separately
Outside, patent document CN102228536A also gives the preparation method of the Chinese medical extract, wherein 60%~70% second of Radix Astragali
Alcohol solution refluxing extraction；Gorgon fruit and the fruit of glossy privet with 80%~90% ethanol solution refluxing extraction；The fruit of Cherokee rose, Common Achyranthes Herb, ghost
Fletching and rheum officinale water boiling and extraction.
It is well known that Chinese medicine compound prescription is particular about the combination principle of monarch and seven feelings, decocting method is also in compliance with certain rule
Then.Qing Dynasty's doctor's Xu Ling tire point out " method boiled medicine is most preferably deep to be said, the benefits of medicine with do not imitate, be this entirely ", it is sufficient to illustrate decoction side
The importance of method.Therefore different extracting methods, the composition ratio of each main chemical compositions of extracting solution are used to Chinese medicine compound prescription
There is larger difference, the change of this chemical component composition ratio, may changing each effective component in Chinese medicine compound prescription, " collaboration is made
With ", as a result lead to the change of the difference or major function of drug effect size.The Chinese medicine group that patent document CN102228536A is mentioned
Although side confirms that in terms for the treatment of diabetic nephropathy, effectively the preparation method that it is provided is not necessarily optimal through clinic
Scheme, it is necessary to be advanced optimized in conjunction with extracting method of the pharmacodynamic test to the prescription, the extract for preparing it plays maximum
Drug effect.
Summary of the invention
The primary purpose of the present invention is that a kind of preparation method of the Chinese medical extract of new treatment diabetic nephropathy is provided,
Chinese medical extract using method preparation has better drug effect in terms for the treatment of diabetic nephropathy.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of preparation method of Chinese medical extract, the bulk pharmaceutical chemicals composition of the Chinese medical extract are as follows: 600~1000 weight of Radix Astragali
Measure part, 400~600 parts by weight of Gorgon fruit, 400~600 parts by weight of the fruit of Cherokee rose, 600~1000 parts by weight of Common Achyranthes Herb, the fruit of glossy privet 400
320~480 parts by weight of~480 parts by weight, 400~480 parts by weight of Ramulus euonymi and rheum officinale；The preparation method includes following step
It is rapid: (1) Radix Astragali, the fruit of glossy privet, kowtow grass be added 6~10 times of medicinal material weight mass concentration be 40%~60% ethanol solution, mention
It takes 1~3 time, 1~2 hour every time, filtration, filtrate recycling ethanol obtained concentrate I；(2) Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale
8~12 times of medicinal material weight of water is added, extracts 1~3 time, 1~2 hour every time, filtration, filtrate concentration obtained concentrate II；(3)
The concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed.
In one embodiment of the invention, the bulk pharmaceutical chemicals composition of the Chinese medical extract are as follows: 600~1000 weight of Radix Astragali
Measure part, 400~480 parts by weight of Gorgon fruit, 400~480 parts by weight of the fruit of Cherokee rose, 600~1000 parts by weight of Common Achyranthes Herb, the fruit of glossy privet 400
320~480 parts by weight of~480 parts by weight, 400~480 parts by weight of Ramulus euonymi and rheum officinale.
In one embodiment of the invention, preparation method of the present invention preferably includes following steps: (1) yellow
Stilbene, the fruit of glossy privet, kowtow grass be added 8 times of medicinal material weight mass concentration be 50% ethanol solution, extract 2 times, 1.5 hours every time,
Filtration, filtrate recycling ethanol obtain concentrate I；(2) 10 times of medicinal material weight of water is added in Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale, mentions
It takes 2 times, 1.5 hours every time, filtration, filtrate concentration obtained concentrate II；(3) concentrate I and step (2) obtained step (1)
Obtained concentrate II is uniformly mixed, spray drying.
In one embodiment of the invention, preparation method of the present invention more preferably includes the following steps: (1) Huang
Stilbene, the fruit of glossy privet, kowtow grass be added 8 times of medicinal material weight mass concentration be 50% ethanol solution, refluxing extraction 2 times, 1.5 is small every time
When, filtration, filtrate decompression concentration and recovery ethyl alcohol obtains concentrate I；(2) medicinal material weight is added in Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale
10 times of water, refluxing extraction 2 times, 1.5 hours every time, filtration, filtrate decompression concentration obtained concentrate II；(3) step (1) is obtained
To the obtained concentrate II of concentrate I and step (2) be uniformly mixed, spray drying.
A second object of the present invention is to provide a kind of Chinese medical extract, which is using preparation side of the invention
Method is prepared:
Third object of the present invention is to provide a kind of Chinese medical extracts of the present invention to treat medicine for treating diabetic nephropathy in preparation
In purposes.
Fourth object of the present invention is to provide a kind of Chinese materia medica preparation, and the Chinese materia medica preparation is will be using preparation side of the invention
Chinese medical extract prepared by method is added or is not added the pharmaceutically acceptable auxiliary material of people and is prepared, and the auxiliary material is selected from medicine
Diluent, excipient, filler, adhesive, wetting agent, disintegrating agent, the sorbefacient of field routine, table activating agent, profit
Lubrication prescription etc..In addition, the preparation is selected from decoction, pill, tablet, capsule, granule, the drug of above-mentioned various dosage forms can be pressed
According to the conventional method preparation of pharmaceutical field.
According to animal pharmacodynamics test, the present composition is in treatment diabetic retinopathy compared with prior art
More preferable drug effect can be played.
Specific embodiment
Following is that in conjunction with specific embodiments, the present invention is further explained.But these embodiments be only limitted to illustrate the present invention and
It is not intended to limit the scope of the invention.The experimental method of specific experiment condition is not specified in the following example, usually according to normal
Rule condition.
Embodiment 1: the traditional chinese medicine composition of the invention and preparation method thereof
Raw material effective prescription:
Radix Astragali 600g, Gorgon fruit 400g, fruit of Cherokee rose 400g, Common Achyranthes Herb 600g, fruit of glossy privet 400g, Ramulus euonymi 400g and rheum officinale
320g。
Preparation method:
(1) Radix Astragali, the fruit of glossy privet, kowtow grass be added 8 times of medicinal material weight mass concentration be 50% ethanol solution, refluxing extraction
2 times, 1.5 hours every time, filtration, filtrate decompression concentration and recovery ethyl alcohol obtained concentrate I, and the paste-forming rate finally measured is 20.3%；
(2) 10 times of medicinal material weight of water is added in Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale, and refluxing extraction 2 times, 1.5 is small every time
When, filtration, filtrate decompression concentration obtains concentrate II, and the paste-forming rate finally measured is 19.8%；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed, spray drying, i.e.,
?.
Embodiment 2: the traditional chinese medicine composition of the invention and preparation method thereof
Raw material effective prescription:
Radix Astragali 600g, Gorgon fruit 400g, fruit of Cherokee rose 400g, Common Achyranthes Herb 600g, fruit of glossy privet 400g, Ramulus euonymi 400g and rheum officinale
320g。
Preparation method:
(1) Radix Astragali, the fruit of glossy privet, to kowtow grass 10 times of medicinal material weight of mass concentration is added be 60% ethanol solution, reflux mentions
It takes 1 time, 1 hour every time, filtration, filtrate decompression concentration and recovery ethyl alcohol obtained concentrate I, and the paste-forming rate finally measured is 16.5%；
(2) Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale are added 8 times of medicinal material weight of water, and refluxing extraction 3 times, 2 hours every time,
Filtration, filtrate decompression concentration, obtains concentrate II, and the paste-forming rate finally measured is 21.7%；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed, spray drying, i.e.,
?.
Embodiment 3: the traditional chinese medicine composition of the invention and preparation method thereof
Raw material effective prescription:
Radix Astragali 600g, Gorgon fruit 400g, fruit of Cherokee rose 400g, Common Achyranthes Herb 600g, fruit of glossy privet 400g, Ramulus euonymi 400g and rheum officinale
320g。
Preparation method:
(1) Radix Astragali, the fruit of glossy privet, kowtow grass be added 6 times of medicinal material weight mass concentration be 40% ethanol solution, refluxing extraction
3 times, 2 hours every time, filtration, filtrate decompression concentration and recovery ethyl alcohol obtained concentrate I, and the paste-forming rate finally measured is 18.9%；
(2) Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale are added 12 times of medicinal material weight of water, and refluxing extraction 1 time, 1 hour every time,
Filtration, filtrate decompression concentration, obtains concentrate II, and the paste-forming rate finally measured is 14.2%；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed, spray drying, i.e.,
?.
Embodiment 4: the traditional chinese medicine composition of the invention and preparation method thereof
Raw material effective prescription:
Radix Astragali 1000g, Gorgon fruit 480g, fruit of Cherokee rose 480g, Common Achyranthes Herb 1000g, fruit of glossy privet 480g, Ramulus euonymi 480g and rheum officinale
400g。
Preparation method:
(1) Radix Astragali, the fruit of glossy privet, kowtow grass be added 8 times of medicinal material weight mass concentration be 50% ethanol solution, refluxing extraction
2 times, 1.5 hours every time, filtration, filtrate decompression concentration and recovery ethyl alcohol obtained concentrate I, and the paste-forming rate finally measured is 19.7%；
(2) 10 times of medicinal material weight of water is added in Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale, and refluxing extraction 2 times, 1.5 is small every time
When, filtration, filtrate decompression concentration obtains concentrate II, and the paste-forming rate finally measured is 18.4%；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed, spray drying, i.e.,
?.
Embodiment 5: the traditional chinese medicine composition of the invention and preparation method thereof
Raw material effective prescription:
Radix Astragali 1000g, Gorgon fruit 480g, fruit of Cherokee rose 480g, Common Achyranthes Herb 1000g, fruit of glossy privet 480g, Ramulus euonymi 480g and rheum officinale
400g。
Preparation method:
(1) Radix Astragali, the fruit of glossy privet, to kowtow grass 10 times of medicinal material weight of mass concentration is added be 60% ethanol solution, reflux mentions
It takes 1 time, 1 hour every time, filtration, filtrate decompression concentration and recovery ethyl alcohol obtained concentrate I, and the paste-forming rate finally measured is 17.1%；
(2) Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale are added 8 times of medicinal material weight of water, and refluxing extraction 3 times, 2 hours every time,
Filtration, filtrate decompression concentration, obtains concentrate II, and the paste-forming rate finally measured is 20.2%；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed, spray drying, i.e.,
?.
Embodiment 6: the traditional chinese medicine composition of the invention and preparation method thereof
Raw material effective prescription:
Radix Astragali 1000g, Gorgon fruit 480g, fruit of Cherokee rose 480g, Common Achyranthes Herb 1000g, fruit of glossy privet 480g, Ramulus euonymi 480g and rheum officinale
400g。
Preparation method:
(1) Radix Astragali, the fruit of glossy privet, kowtow grass be added 6 times of medicinal material weight mass concentration be 40% ethanol solution, refluxing extraction
3 times, 2 hours every time, filtration, filtrate decompression concentration and recovery ethyl alcohol obtained concentrate I, and the paste-forming rate finally measured is 18.2%；
(2) Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale are added 12 times of medicinal material weight of water, and refluxing extraction 1 time, 1 hour every time,
Filtration, filtrate decompression concentration, obtains concentrate II, and the paste-forming rate finally measured is 14.6%；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed, spray drying, i.e.,
?.
Comparative example 1
Raw material effective prescription:
Radix Astragali 600g, Gorgon fruit 400g, fruit of Cherokee rose 400g, Common Achyranthes Herb 600g, fruit of glossy privet 400g, Ramulus euonymi 400g and rheum officinale
320g。
Preparation method: with patent document CN102228536A [0035] section Chinese medical extract preparation method.
Comparative example 2
Raw material effective prescription:
Radix Astragali 1000g, Gorgon fruit 480g, fruit of Cherokee rose 480g, Common Achyranthes Herb 1000g, fruit of glossy privet 480g, Ramulus euonymi 480g and rheum officinale
400g。
Preparation method: with patent document CN102228536A [0107] section Chinese medical extract preparation method.
The influence for the Type I diabetes renal lesions rat that experimental example induces streptozotocin (STZ)
1. experimental material
(1) experimental animal
SPF grades of SD rats, 180-220g are purchased from Beijing HFK Bio-Technology Co., Ltd., raise in Beijing also
Village international bio Pharmaceutical Technology Co., Ltd.
(2) experimental drug
Chinese medical extract prepared by embodiment 1, embodiment 4, comparative example 1 and comparative example 2.
(3) experiment reagent
Streptozotocin；Blood sugar test paper (glucose dehydrogenase method)；Glucose；Chloraldurate；Quantity of proteinuria testing cassete,
Albumin testing cassete, microalbumin testing cassete, 4% paraformaldehyde.
(4) experimental material
96 orifice plates；Intravenous infusion needle (7#)；5mL heparin tube (common tube).
(5) laboratory apparatus
The complete brilliant wonderful type blood glucose meter (U.S.'s Roche) of Luo Kang；AL204 type electronic balance (Mei Tele-support benefit instrument (on
Sea) Co., Ltd)；MB100-4A type microwell plate constant temperature oscillator (Hangzhou Ao Sheng Instrument Ltd.)；HC-2518 type high speed cold
Freeze centrifuge (Anhui Zhong Kezhongjia scientific instrument Co., Ltd)；MULTISKAN MK3 type microplate reader (Thermo company, the U.S.)；
DW-HL218 type ultra low temperature freezer (Zhong Kemeiling low temperature Science and Technology Co., Ltd.).
2. experimental method
8 SPF grades of week old SD rats are raised in IVC grades of Experimental Animal Centers.After health quarantine 5 days, it is randomly divided by weight
Normal group and model group, after fasting 10 hours, model group presses the abdominal cavity 55mg/kg one injection 1%STZ citrate buffer solution,
Normal group injection equivalent citrate buffer solution, fasting 3h after injection 72 hours detect fasting blood-glucose.After glucostasis 4 weeks, with
Fasting blood-glucose > 16.7mmo/L person enters group.Continue raising 12~16~20~24 weeks.The successful standard of DN model need to meet on an empty stomach
Blood glucose > 16.7mmo/L and Mogensen III phase standard.4 weeks after STZ injection, according to preceding blood glucose surveyed several times, reject any one
The rat of secondary blood glucose < 11.1mmoL/L, remaining rat are Type I diabetes renal lesions rat model.
Type I diabetes renal lesions rat model is grouped at random by blood glucose, weight and urine index, be divided into model group and to
Medicine group, wherein model group (10), administration group are divided into example 1 group (12), 4 groups of embodiment (12), 1 group (12 of comparative example
Only), 2 groups of comparative example (12) carries out craft screening pharmacodynamic test.Administration group is with 6g crude drug/kg stomach-filling relative medicine, and normal group
With model group stomach-filling equal amount of distilled water, one time a day, week for 7 weeks.
Observation index: weight, fasting blood-glucose, urinary protein concentrations, urine albumin concentration, UAE, microdose urine protein concentration.
Continuous data is for statistical analysis using SPSS16.0 software, and experimental data is indicated with x ± SD, and more comparison among groups are adopted
It is that difference is statistically significant with One-Way Anova, P < 0.05.
3. experimental data and experimental result
Experimental result is shown in such as the following table 1.
Influence (x ± SD) of 1 Chinese medical extract of table to Type I diabetes renal lesions rat model weight and urine biochemistry
Note: compared with normal group, * P < 0.05；Compared with model group, ◆ P < 0.05.
After STZ is injected 4 weeks, that is, it is administered 0 week, compared with normal group, model group rats weight loss；Urinary protein concentrations, urine
Albumin concentration, urinary albumin excretion ratio (UAE) and microdose urine protein concentration rise；Difference has statistical significance (P <
0.05), 1 type SD rat DN model modeling of prompt success.After drug gavages 4 weeks, compared with model group, example 1 group and implementation
4 groups of urinary protein concentrations of example, urine albumin concentration, urinary albumin excretion ratio (UAE) and microdose urine protein concentration reduce, difference tool
Statistically significant (P < 0.05)；And the Chinese medical extract urinary protein concentrations, the white egg of urine of 2 groups of preparations of 1 group of comparative example and comparative example
White concentration, urinary albumin excretion ratio (UAE) and microdose urine protein concentration also decrease, but effect is obviously not so good as the present invention
Chinese medical extract.Prompt, the Chinese medical extract of the method for the present invention preparation are better than the prior art to the therapeutic effect of diabetic nephropathy
The Chinese medical extract of preparation.
It should be understood that after reading the above teachings of the present invention, those skilled in the art can make the present invention
Various changes or modification, these equivalent forms also fall within the scope of the appended claims of the present application.
Claims (8)
1. a kind of preparation method of Chinese medical extract, the bulk pharmaceutical chemicals composition of the Chinese medical extract are as follows:
600~1000 parts by weight of Radix Astragali, 400~600 parts by weight of Gorgon fruit, 400~600 parts by weight of the fruit of Cherokee rose, Common Achyranthes Herb 600~
1000 parts by weight, 320~480 parts by weight of 400~480 parts by weight of the fruit of glossy privet, 400~480 parts by weight of Ramulus euonymi and rheum officinale；
It is characterized in that, the preparation method includes the following steps:
(1) Radix Astragali, the fruit of glossy privet, kowtow grass be added 6~10 times of medicinal material weight mass concentration be 40%~60% ethanol solution, mention
It takes 1~3 time, 1~2 hour every time, filtration, filtrate recycling ethanol obtained concentrate I；
(2) 8~12 times of medicinal material weight of water is added in Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale, extracts 1~3 time, 1~2 is small every time
When, filtration, filtrate concentration obtains concentrate II；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed.
2. a kind of preparation method of Chinese medical extract, it is characterised in that the bulk pharmaceutical chemicals of the Chinese medical extract form are as follows:
600~1000 parts by weight of Radix Astragali, 400~480 parts by weight of Gorgon fruit, 400~480 parts by weight of the fruit of Cherokee rose, Common Achyranthes Herb 600~
1000 parts by weight, 320~480 parts by weight of 400~480 parts by weight of the fruit of glossy privet, 400~480 parts by weight of Ramulus euonymi and rheum officinale.
3. a kind of preparation method as claimed in claim 1 or 2, it is characterised in that the preparation method includes the following steps:
(1) Radix Astragali, the fruit of glossy privet, kowtow grass be added 8 times of medicinal material weight mass concentration be 50% ethanol solution, extract 2 times, every time
1.5 hours, filtration, filtrate recycling ethanol obtained concentrate I；
(2) 10 times of medicinal material weight of water is added in Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale, extracts 2 times, 1.5 hours every time, filters,
Filtrate concentration, obtains concentrate II；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed, spray drying.
4. a kind of preparation method as claimed in claim 3, it is characterised in that the preparation method includes the following steps:
(1) Radix Astragali, the fruit of glossy privet, kowtow grass be added 8 times of medicinal material weight mass concentration be 50% ethanol solution, refluxing extraction 2 times,
1.5 hours every time, filtration, filtrate decompression concentration and recovery ethyl alcohol obtained concentrate I；
(2) 10 times of medicinal material weight of water is added in Gorgon fruit, the fruit of Cherokee rose, Ramulus euonymi, rheum officinale, refluxing extraction 2 times, 1.5 hours every time, filters
It crosses, filtrate decompression concentration obtains concentrate II；
(3) concentrate I that step (1) obtains and the concentrate II that step (2) obtains are uniformly mixed, spray drying.
5. a kind of Chinese medical extract, it is characterised in that the extract is using the preparation method as described in power 1 to 4 any one of power
It is prepared.
6. a kind of application of Chinese medical extract as claimed in claim 5 in preparation treatment medicine for treating diabetic nephropathy.
7. a kind of Chinese materia medica preparation, it is characterised in that the preparation is the Chinese medical extract as described in claim 5, is added or is not added
The pharmaceutically acceptable auxiliary material of people is prepared.
8. a kind of Chinese materia medica preparation as claimed in claim 7, it is characterised in that the preparation is selected from decoction, pill, tablet, capsule
Agent, granule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811541786.2A CN109549975A (en) | 2018-12-17 | 2018-12-17 | A kind of preparation method of Chinese medical extract and its Chinese medical extract of preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811541786.2A CN109549975A (en) | 2018-12-17 | 2018-12-17 | A kind of preparation method of Chinese medical extract and its Chinese medical extract of preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109549975A true CN109549975A (en) | 2019-04-02 |
Family
ID=65870198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811541786.2A Withdrawn CN109549975A (en) | 2018-12-17 | 2018-12-17 | A kind of preparation method of Chinese medical extract and its Chinese medical extract of preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109549975A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110755487A (en) * | 2019-10-08 | 2020-02-07 | 北京汉典制药有限公司 | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228536A (en) * | 2011-06-09 | 2011-11-02 | 首都医科大学 | Chinese herbal extract for treating diabetic nephropathy and preparation method thereof |
-
2018
- 2018-12-17 CN CN201811541786.2A patent/CN109549975A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228536A (en) * | 2011-06-09 | 2011-11-02 | 首都医科大学 | Chinese herbal extract for treating diabetic nephropathy and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| ***等: "《新编常用中药有效成分手册》", 31 January 2008, 中国协和医科大学出版社 * |
| 杨玉霞等: "《中国临床药物大辞典·中药饮片卷》", 31 August 2018, 中国健康传媒集团 中国医药科技出版社 * |
| 陈蓉等: "不同产地芡实药材水溶性蛋白质的含量分析", 《中国药房》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110755487A (en) * | 2019-10-08 | 2020-02-07 | 北京汉典制药有限公司 | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102228536B (en) | Chinese herbal extract for treating diabetic nephropathy and preparation method thereof | |
| CN108853433A (en) | A kind of Chinese medicine and preparation method thereof for treating diabetic nephropathy | |
| CN104173503B (en) | The Chinese herbal granules of the preparation method of a kind of Chinese herbal granules and preparation thereof and purposes | |
| CN109549975A (en) | A kind of preparation method of Chinese medical extract and its Chinese medical extract of preparation | |
| CN109512875A (en) | A kind of preparation method of Chinese medical extract and its Chinese medical extract of preparation | |
| CN104721467B (en) | Traditional Chinese medicine composition for treating diabetic nephropathy and application thereof | |
| CN102671060A (en) | Medicinal composition for treating gout and preparation method thereof | |
| CN102631526A (en) | Chinese medicinal composition for treating diabetes mellitus | |
| CN100333758C (en) | Gout resisting Chinese medicine composition and its prepn process | |
| CN103211996B (en) | Preparation method of traditional Chinese medicine for treating diabetes mellitus | |
| CN103432420B (en) | A kind of Chinese medicine composition for the treatment of diabetes and preparation method thereof and detection method | |
| CN108714205A (en) | A kind of Chinese medicinal granule and its preparation method and application for treating yang deficiency of spleen and stomache IgA nephrosis | |
| CN101829271B (en) | Chinese medicinal compound with function of treating diabetes and preparation method and application thereof | |
| CN104161850A (en) | Preparation method of Chinese herbal extract, prepared Chinese herbal extract and application | |
| CN1712055A (en) | Chinese medicinal preparation (litholytic capsule) for treating urinific calculus | |
| CN106853021A (en) | Prevent and treat Chinese medicine composition of CKD and its preparation method and application | |
| CN106421523B (en) | A kind of Chinese medicine compound prescription for treating gout | |
| CN100531749C (en) | Nephropathy treating medicine composition and its preparation process | |
| CN103446494B (en) | Chinese patent medicine for treating Type II diabetic nephropathy and preparation method thereof | |
| CN104547514A (en) | Traditional Chinese medicine composition for treating systemic lupus erythematosus rheumatoid arthritis vasculitis and application thereof | |
| CN109793746A (en) | It is a kind of to improve the composition of renal insufficiency, black conopsea extraction and its preparation method and application | |
| CN104173506A (en) | Traditional Chinese medicine extract, as well as preparation method and application thereof | |
| CN104173505B (en) | The preparation method of a kind of Chinese medicinal compound extract and the Chinese medicinal compound extract of preparation thereof and purposes | |
| CN107669860A (en) | A kind of Chinese medicine composition and its application with blood sugar reducing function | |
| CN103083610B (en) | Chronic glomerulonephritis treatment drug |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190402 |
|
| WW01 | Invention patent application withdrawn after publication |