CN109540854B - Nano metal structure for enhancing near infrared band fluorescence signal and preparation method thereof - Google Patents
Nano metal structure for enhancing near infrared band fluorescence signal and preparation method thereof Download PDFInfo
- Publication number
- CN109540854B CN109540854B CN201811304206.8A CN201811304206A CN109540854B CN 109540854 B CN109540854 B CN 109540854B CN 201811304206 A CN201811304206 A CN 201811304206A CN 109540854 B CN109540854 B CN 109540854B
- Authority
- CN
- China
- Prior art keywords
- fluorescence
- molecule
- metal structure
- fluorescent
- metal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
Abstract
The invention belongs to the technical field of biophysical single-molecule fluorescence enhancement, and particularly relates to an asymmetric nano metal structure for effectively enhancing a near-infrared band single-molecule fluorescence signal and a preparation method thereof. The asymmetric metal nano structure provided by the invention has a plurality of resonance modes, the radiation property and the energy dissipation property of a structure-molecule system can be modulated by adjusting the coupling between the modes, the quantum efficiency of fluorescent molecules in a radiation wave band is improved, and the overall fluorescent signal is enhanced. The structural design provided by the invention can meet the signal enhancement requirement of fluorescent molecules in a near infrared band (900-1700 nm), and the provided preparation method can process the nano structure with high precision and high efficiency, and has higher application value in the field of biomedical detection.
Description
Technical Field
The invention belongs to the technical field of biophysical single-molecule observation, and particularly relates to a metal nanostructure for enhancing a near-infrared band single-molecule fluorescence signal.
Background
The single molecule experiment is an observation technique which is attracting attention in the current biological application, for example, a method of labeling and observing a single target molecule with a fluorescent dye or fluorescent protein, etc., and information which has been averaged and annihilated in the past in a multi-molecule experiment system can be obtained [1] . Meanwhile, compared with the fluorescence technology of visible wave band, near Infrared (NIR) fluorescence has great application in the biomedical field due to the interference of larger tissue penetration depth, less tissue absorption, autofluorescence and the likePotential of use [2]. However, we have to look ahead at the problem that near infrared molecular fluorescence is generally low in quantum yield and light sensitive, and fluorescence bleaching is likely to occur, and these limiting factors result in a single-molecule fluorescence signal intensity that is generally weak. To meet the intensity requirements of signal analysis, fluorescence enhancement techniques have been developed.
Currently, the single-molecule fluorescence enhancement techniques that have been widely used are of the following main categories: 1) The composition and modification of the fluorescent particles are modified to improve the luminescence properties thereof, including but not limited to modification and modification of fluorescent materials such as fluorescent quantum dots, rare earth fluorescent particles, carbon nanotubes and the like. The fluorescent quantum dots have stable luminescence property due to higher quantum yield, and have been widely applied to the field of medical imaging. However, the fluorescent material obtained by the method has large monomer volume, strong biotoxicity and complex modification to adapt to application requirements, and the defect of biocompatibility limits the further application of the fluorescent material [2] . 2) The structure of fluorescent molecules such as fluorescent protein, fluorescent dye and the like is modified to improve the luminescence property of the fluorescent molecules. Fluorescent proteins and dye molecules are considered to have great potential for use because of their small size, their lack of restriction on the physiological activity of the tagged subject, and their low biotoxicity. At present, the quantum yield is improved mainly by modifying the amino acid sequence of fluorescent protein, the chemical structure of fluorescent dye and optimizing the molecular chemical environment. However, the quantum yield of the near infrared fluorescent molecules is too low, the modified molecular quantum yield is still low and is obviously lower than that of the quantum dot fluorescent particles, and the molecules are usually photosensitive and easy to photobleaching, so that the near infrared fluorescent molecules are still difficult to widely apply at present [3][4] . 3) Based on the surface plasmon effect of the monomer, symmetrical or multilayer nano metal structure, the excitation speed of the adjacent fluorescent molecules is effectively improved by utilizing the highly localized strong excitation electromagnetic field formed by the hot spot area. Such techniques can obtain more fluorescent signal from this molecule per unit time, resulting in a significant enhancement of the overall signal [5] . But is not suitable for fluorescent molecules with lower quantum yields or easy bleaching per se because it mainly increases the excitation efficiency.
By combining the limiting factors, a method suitable for enhancing the near infrared band fluorescent molecular signals is developed, and the fluorescence intensity is effectively enhanced on the premise of not accelerating the fluorescence bleaching process, so that the method has very important significance and application value.
Disclosure of Invention
The invention aims to overcome the defects of the existing near infrared single-molecule fluorescence enhancement technology and provides a nano metal structure for enhancing fluorescence quantum yield and thereby enhancing a near infrared band single-molecule fluorescence signal.
The nano metal structure for enhancing the near infrared band single-molecule fluorescence signal provided by the invention uses an asymmetric double-rod structure, so that the quantum yield of fluorescent molecules in an adjacent area can be effectively enhanced, and a stronger fluorescence signal can be obtained in unit time; meanwhile, the photobleaching of fluorescent molecules is inhibited, the observation time of single molecules is effectively prolonged, and therefore the luminous performance of the fluorescent molecules is optimized.
According to the invention, under the guidance of a theoretical model, the radiation property and the energy dissipation property of the system are adjusted by designing a plurality of resonance modes of the composite metal nanostructure, and the fluorescence emission frequency of molecules is improved as much as possible, and meanwhile, the absorption and dissipation of the system are reduced as much as possible, so that the quantum efficiency of fluorescent molecules in a radiation wave band is finally improved, and the fluorescence bleaching time is effectively prolonged while the overall fluorescence signal is enhanced.
The parameters of the nano metal structure provided by the invention are shown in the following table (the structural schematic diagram and the parameter marks are shown in the attached figure 1):
| molecular radiation wavelength (nm) | 900 | 1000 | 1100 | 1200 | 1300 | 1400 | 1500 | 1600 | 1700 |
| b1(nm) | 80-90 | 110-130 | 150-170 | 170-190 | 180-200 | 200-220 | 220-240 | 250-260 | 270-290 |
| b2(nm) | 120-130 | 160-180 | 200-220 | 220-240 | 220-240 | 240-260 | 260-290 | 280-290 | 300-310 |
* The length of the shorter metal rod is b1, and the length of the longer metal rod is b2.
* The distance between the two bars of the fixed structure is 40nm, and under the excitation condition of 760nm laser, the structural parameters can be obtained through simulation calculation by taking the total fluorescence enhancement multiple of 100 times as the lower limit.
The invention provides a preparation method of a nano metal structure for enhancing a near infrared band single-molecule fluorescent signal, which comprises the following specific steps:
(a) Transparent glass sheets are selected as a substrate (preferably with the thickness of 0.15-0.20 mm), concentrated sulfuric acid and hydrogen peroxide mixture (generally, the weight ratio of the concentrated sulfuric acid to the hydrogen peroxide mixture is preferably 2:1-4:1) are used for surface cleaning, and the substrate is favorable for subsequent fluorescence observation;
(b) Blowing the glass sheet with high-purity nitrogen, coating a glue layer PMMA (namely polymethyl methacrylate) by using a spin coating method, steaming an aluminum film (the film thickness is preferably 5-10 nm) by using an electron beam method to enhance the conductivity of a sample and improve the processing precision, and then carrying out electron beam Exposure (EBL) on a proper area of the glass sheet to write out a designed nano-structure array;
(c) Soaking the treated article with chemical solution to eliminate aluminum film; (the chemical solution is preferably a mixed solution of 3038 positive photoresist developer and deionized water, and the weight ratio of the 3038 positive photoresist developer to the deionized water is 1:3 to 1:5);
(d) Removing structural part PMMA by using a developing solution, pre-cooling the developing solution to 0 ℃, and developing for 30-40min at low temperature, wherein the developing solution is a mixed solution of MIBK and IPA, and the weight ratio of MIBK to IPA in the mixed solution is 1:2 to 1:4; the low-temperature development is beneficial to controlling the development speed and improving the development precision;
(e) Evaporating a metal layer (preferably gold or silver) by an electron beam method to form a metal structure array in a development area, wherein the evaporating thickness is 35-45nm, preferably about 40nm; soaking with acetone, and removing redundant metal-PMMA film with the assistance of ultrasound;
(f) Photographing under an electron microscope, and determining the integrity and structural condition of the array;
(g) The far field transmission spectrum of the array of structures is tested to ensure that the structural response is consistent with the design.
In the experimental process, after fluorescent molecules positioned in the adjacent area of the metal structure are excited, a certain proportion of energy is transmitted to the metal structure at the radiation frequency, the metal structure radiates the energy in a photon form, the fluorescence quantum yield of the system is far higher than the radiation efficiency of the molecules, and the single-molecule fluorescence signal is obviously enhanced; in the single excitation process, as the number of the molecular excited state energy outflow channels is increased, the proportion of the fluorescence bleaching channels of one channel is reduced, and the fluorescence bleaching probability is reduced, so that the excitation times which can be experienced by a single molecule are increased, and the fluorescence observation time is effectively prolonged. The nanostructure designed by the patent can obviously improve the luminous property of the near infrared band fluorescent molecule.
The nano metal structure provided by the invention can meet the signal enhancement requirement of fluorescent molecules in a near infrared band (900-1700 nm), and the provided preparation method can process the nano structure with high precision and high efficiency, and has higher application value in the field of biomedical detection.
Drawings
Fig. 1 is a schematic diagram of a metal nanostructure design, and structural dimension parameters are labeled as shown in the figure.
FIG. 2 is a schematic diagram of a single-molecule fluorescence experimental device and an observation mode.
FIG. 3 is an electron microscope scan of a nano-metal structure used in AIEE1000 fluorescence enhancement experiments, indicating the dimensions of the metal structure.
Fig. 4 shows absorption spectrum, emission spectrum and far-field response spectrum of the fluorescent molecule AIEE1000, and the structure has obvious response in the emission band of the fluorescent molecule, and the embedded graph shows the chemical structure diagram of the AIEE 1000.
Fig. 5 shows the fluorescence imaging effect of fluorescent molecule AIEE1000 in the unstructured coverage area and the change in fluorescence intensity with time at the corresponding location.
Fig. 6 shows the fluorescence imaging effect of fluorescent molecule AIEE1000 over the area of coverage of the structure and the change in fluorescence intensity over time at the corresponding locations.
FIG. 7 shows the absorption spectrum and emission spectrum of fluorescent molecule CH1055 and the far field response spectrum of metal structure, the structure has obvious response in the emission band of fluorescent molecule, and the embedded graph is the chemical structure diagram of CH 1055.
Fig. 8 is a graph showing the fluorescence imaging effect of fluorescent molecule CH1055 in the non-structural coverage area and the change of fluorescence intensity with time at the corresponding position.
Fig. 9 is a graph showing the fluorescence imaging effect of fluorescent molecule CH1055 in the coverage area of the structure and the change of fluorescence intensity with time at the corresponding position.
Detailed Description
The invention is further described below with reference to the drawings and examples.
Example 1: structure enhancement of AIEE1000 single molecule fluorescence signal
1. The nanostructure for fluorescence enhancement (structure size b1=150 nm, b2=210 nm, gap=40 nm, h=40 nm, width=40 nm) was selected according to the absorption spectrum, radiation spectrum of the fluorescent molecule AIEE1000, and the structure array was prepared according to the method described in the specific steps (a) - (g) in the "summary of the invention". The chemical structure diagram of the AIEE1000, the scanning result of an electron microscope of the structure, the absorption spectrum and the emission spectrum of the AIEE1000 and the far-field response spectrum of the prepared metal nanostructure array are shown in fig. 3 and 4;
2. dissolving fluorescent molecules in PMMA at a concentration of about 10uM, spin-coating the solution on the surface of a clean glass sheet by using a spin coater at a rotating speed of 4000rpm to form a fluorescent molecule film with a thickness of about 80nm, wherein the final sample morphology is shown schematically in FIG. 2;
3. the fluorescence intensity of AIEE1000 molecules in the non-structural area and the structural area is observed in sequence under a total internal reflection fluorescence microscope (TIRF) by using 633nm laser to excite the molecules, and the enhancement multiple of the fluorescence signal of a single molecule can be obtained by comparing the fluorescence intensity of the single molecule in the two areas, and the experimental device is shown in figure 2.
FIG. 5 shows the results of fluorescence imaging of unstructured regions and the change in single-point fluorescence intensity over time. The single-molecule fluorescence signal is weak because the fluorescence quantum yield of the fluorescent molecule AIEE1000 is low (about 1.8%), and the response efficiency of the detection camera in the fluorescence radiation band is low. Comprehensive statistics of single-molecule levels of fluorescent molecules in multiple sample areas and non-structural areasAverage fluorescence intensity I 0 Only 53.04 photons/sec; FIG. 6 shows the results of fluorescence imaging of structural regions and the change in single-point fluorescence intensity over time. Identifying and calculating the fluorescence intensity I of single molecules in the adjacent area of the structure by a program, and using the fluorescence intensity I of single molecules in the non-structural area 0 As a reference, the fluorescence enhancement factor e=i/I for each molecule in the vicinity of the structural region can be calculated 0 The fluorescence enhancement factor Emean value is 78.7 times, and the highest value can reach 405 times.
Example 2: enhancement of CH1055 single molecule fluorescence signal by structure
1. The nanostructure for fluorescence enhancement (structure size b1=150 nm, b2=210 nm, gap=40 nm, h=40 nm, width=40 nm) was selected according to the absorption spectrum, radiation spectrum of the fluorescent molecule CH1055, and the structure array was prepared according to the method described in the specific steps (a) - (g) in the "summary of the invention". The metal nanostructure used here was the same as in example 1, and the electron microscope scanning results are shown in fig. 3. The chemical structure diagram, absorption spectrum and emission spectrum of CH1055 and the far-field response spectrum of the prepared metal nanostructure array are shown in figure 7;
2. dissolving fluorescent molecules in PMMA at a concentration of about 12uM, spin-coating the solution on the surface of a clean glass sheet by using a spin coater at a rotating speed of 4000rpm to form a fluorescent molecule film with a thickness of about 80nm, wherein the final sample morphology is shown schematically in FIG. 2;
3. the fluorescence intensity of the non-structural region and the structural region CH1055 molecule is observed in sequence under a total internal reflection fluorescence microscope (TIRF) by using 633nm laser to excite the molecules, and the enhancement multiple of the single-molecule fluorescence signal can be obtained by comparing the fluorescence intensity of the single molecules in the two regions, and the experimental device is shown in figure 2.
FIG. 8 shows the results of fluorescence imaging of unstructured regions and the change in single-point fluorescence intensity over time. The single-molecule fluorescence signal is weak because the fluorescence quantum yield of fluorescent molecule CH1055 is low (about 6.9%), and the response efficiency of the detection camera in the fluorescence radiation band is low. Comprehensively counting single-molecule average fluorescence intensity I of fluorescent molecules in a plurality of sample areas and non-structural areas 0 About 325.89 photons/sec; FIG. 9 shows structural area fluorescence imaging results and single point fluorescence intensityTime-dependent changes. Identifying and calculating the fluorescence intensity I of single molecules in the adjacent area of the structure by a program, and using the fluorescence intensity I of single molecules in the non-structural area 0 As a reference, the fluorescence enhancement factor e=i/I for each molecule in the vicinity of the structural region can be calculated 0 The mean value of the fluorescence enhancement factor Emean is 19.8 times, and the maximum is 67 times.
Reference is made to:
1. Cang H, Labno A, Lu C, et al. Probing the electromagnetic field of a 15-nanometre hotspot by single molecule imaging[J]. Nature, 2011, 469(7330): 385.
2. Hong G, Antaris A L, Dai H. Near-infrared fluorophores for biomedical imaging[J]. Nature Biomedical Engineering, 2017, 1(1): 0010.
3. Antaris A L, Chen H, Cheng K, et al. A small-molecule dye for NIR-II imaging[J]. Nature materials, 2016, 15(2): 235.
4. Qian G, Dai B, Luo M, et al. Band gap tunable, donor− acceptor− donor charge-transfer heteroquinoid-based chromophores: Near infrared photoluminescence and lectroluminescence[J]. Chemistry of Materials, 2008, 20(19): 6208-6216.
5.Li J F, Li C Y, Aroca R F. Plasmon-enhanced fluorescence spectroscopy[J]. Chemical Society Reviews, 2017, 46(13): 3962-3979.。
Claims (6)
1. the preparation method of the nano metal structure for enhancing the near infrared band fluorescence signal is characterized by comprising the following specific steps:
(a) Selecting a transparent glass sheet as a substrate, and cleaning the surface of the substrate by using a mixture of concentrated sulfuric acid and hydrogen peroxide;
(b) Blowing the glass sheet with high-purity nitrogen, coating a glue layer PMMA with a spin coating method, steaming an aluminum film with an electron beam method to enhance the conductivity of a sample and improve the processing precision, and then carrying out electron beam exposure on a proper area of the glass sheet to write out a designed nanostructure array;
(c) Soaking the treated article with chemical solution to eliminate aluminum film;
(d) Developing a structural part PMMA by using a developing solution, pre-cooling the developing solution to 0 ℃, and developing for 30-40min at a low temperature, wherein the developing solution is a mixed solution of MIBK and IPA, and the weight ratio of MIBK to IPA in the mixed solution is 1 (2-4);
(e) Evaporating a metal layer by an electron beam method, forming a metal structure array in a developing area, wherein the evaporating thickness is 40-60nm; soaking with acetone, and removing excessive metal-PMMA composite film with the assistance of ultrasound;
the nano metal structure is a head-to-head double-rod-shaped metal structure with asymmetric length, the length of a shorter metal rod is b1, and the length of a longer metal rod is b2; the distance between the two bars of the fixed structure is 40nm, and under the excitation condition of 760nm laser, the overall fluorescence enhancement multiple is 100 times as a lower limit, and the structural parameters are obtained through simulation calculation as shown in the following table:
。
2. The method of claim 1, wherein the glass sheet in step (a) has a thickness of 0.15 to 0.20mm; the weight ratio of the concentrated sulfuric acid to the hydrogen peroxide is (2-4) 1.
3. The method according to claim 1, wherein the thickness of the aluminum film evaporated in the step (b) is 5 to 10nm.
4. The method of claim 1, wherein the chemical solution used in step (c) is a mixture of 3038 positive photoresist developer and deionized water, and the weight ratio of 3038 positive photoresist developer to deionized water is 1 (3-5).
5. The method of claim 1, wherein the metal layer material in step (e) is gold or silver.
6. An asymmetric nano-metal structure effective to enhance near infrared band single-molecule fluorescence signals obtained by the preparation method of any one of claims 1 to 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811304206.8A CN109540854B (en) | 2018-11-03 | 2018-11-03 | Nano metal structure for enhancing near infrared band fluorescence signal and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811304206.8A CN109540854B (en) | 2018-11-03 | 2018-11-03 | Nano metal structure for enhancing near infrared band fluorescence signal and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109540854A CN109540854A (en) | 2019-03-29 |
| CN109540854B true CN109540854B (en) | 2023-06-27 |
Family
ID=65845974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811304206.8A Active CN109540854B (en) | 2018-11-03 | 2018-11-03 | Nano metal structure for enhancing near infrared band fluorescence signal and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109540854B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110749580B (en) * | 2019-09-18 | 2022-05-10 | 东南大学 | Nanometer microarray near-field structure for monomolecular fluorescence limited-domain excitation |
| CN114018881B (en) * | 2021-10-25 | 2023-04-07 | 南京大学 | Method for detecting local acoustic vibration by plasmon enhanced monomolecular fluorescence system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1957245A (en) * | 2004-05-19 | 2007-05-02 | Vp控股有限公司 | Optical sensor with layered plasmon structure for enhanced detection of chemical groups by sers |
| JP2008007815A (en) * | 2006-06-29 | 2008-01-17 | Nippon Telegr & Teleph Corp <Ntt> | Metal nanoparticulate composite, method for producing the same, metal nanoparticulate composite thin film and method for producing the same |
| JP2010197746A (en) * | 2009-02-25 | 2010-09-09 | Ricoh Co Ltd | Multiphoton absorbing material, reaction aid, and method of manufacturing these |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9823246B2 (en) * | 2011-12-28 | 2017-11-21 | The Board Of Trustees Of The Leland Stanford Junior University | Fluorescence enhancing plasmonic nanoscopic gold films and assays based thereon |
| US20160146799A1 (en) * | 2014-11-05 | 2016-05-26 | Nirmidas Biotech, Inc. | Metal composites for enhanced imaging |
| CN104568876B (en) * | 2014-12-24 | 2017-03-29 | 复旦大学 | The method that Graphene fluorescent quenching carries out fluorescence observation with reference to nano metal array |
| JP6468572B2 (en) * | 2017-06-13 | 2019-02-13 | 国立研究開発法人物質・材料研究機構 | Measuring method and measuring apparatus using array type sensor using enhanced electromagnetic field |
-
2018
- 2018-11-03 CN CN201811304206.8A patent/CN109540854B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1957245A (en) * | 2004-05-19 | 2007-05-02 | Vp控股有限公司 | Optical sensor with layered plasmon structure for enhanced detection of chemical groups by sers |
| JP2008007815A (en) * | 2006-06-29 | 2008-01-17 | Nippon Telegr & Teleph Corp <Ntt> | Metal nanoparticulate composite, method for producing the same, metal nanoparticulate composite thin film and method for producing the same |
| JP2010197746A (en) * | 2009-02-25 | 2010-09-09 | Ricoh Co Ltd | Multiphoton absorbing material, reaction aid, and method of manufacturing these |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109540854A (en) | 2019-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gu et al. | High-sensitivity imaging of time-domain near-infrared light transducer | |
| Gorris et al. | Perspectives and challenges of photon-upconversion nanoparticles-Part II: bioanalytical applications | |
| Gnach et al. | Lanthanide-doped up-converting nanoparticles: Merits and challenges | |
| Vo-Dinh et al. | Plasmonic nanoprobes: from chemical sensing to medical diagnostics and therapy | |
| Yang et al. | A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking | |
| Joyce et al. | Recent advances in gold-based metal enhanced fluorescence platforms for diagnosis and imaging in the near-infrared | |
| Williams et al. | Interpreting second-harmonic generation images of collagen I fibrils | |
| EP2504687B1 (en) | System for detecting metal enhanced fluorescence from metallic nanoburger structures | |
| Zhan et al. | Optimization of optical excitation of upconversion nanoparticles for rapid microscopy and deeper tissue imaging with higher quantum yield | |
| CN105891170B (en) | Living animal two-photon excitation delay detection fluorescence imaging analysis method and apparatus | |
| US20080066549A1 (en) | Methods, systems and apparatus for light concentrating mechanisms | |
| CN109540854B (en) | Nano metal structure for enhancing near infrared band fluorescence signal and preparation method thereof | |
| Li et al. | Fluorescence enhancement enabled by nanomaterials and nanostructured substrates: A brief review | |
| Gartia et al. | Enhanced 3D fluorescence live cell imaging on nanoplasmonic substrate | |
| Solhi et al. | Recent advances on the biosensing and bioimaging based on polymer dots as advanced nanomaterial: Analytical approaches | |
| CN103837499B (en) | A kind of micro-section spectral measurement device based on wideband surface plasma wave | |
| WO2011155435A1 (en) | Near field-enhanced fluorescence sensor chip | |
| Qian et al. | High contrast 3-D optical bioimaging using molecular and nanoprobes optically responsive to IR light | |
| Zhang et al. | WaveFlex biosensor: a flexible-shaped plasmonic optical fiber sensor for histamine detection | |
| CN105286803A (en) | Method for enhancing complex probe photoacoustic signals on basis of fluorescence quenching effect | |
| Lin et al. | In vivo detection of single-walled carbon nanotubes: progress and challenges | |
| Aslan et al. | Microwave-Accelerated Surface Plasmon-Coupled Directional Luminescence: Application to fast and sensitive assays in buffer, human serum and whole blood | |
| Yang et al. | Nanoscale 3D temperature gradient measurement based on fluorescence spectral characteristics of the CdTe quantum dot probe | |
| JP2014228322A (en) | Sensor and inspection method | |
| TWI723508B (en) | Method of forming enhanced super-resolution image |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |