CN109536404A - A kind of feeding bacillus pumilus and its application - Google Patents
A kind of feeding bacillus pumilus and its application Download PDFInfo
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Abstract
The invention discloses a kind of feeding bacillus pumilus (Bacillus pumilus) 13824, deposit number is CGMCC NO.16539.Bacillus pumilus 13824 of the invention is G+ bacteria, simultaneously resistance to 80 DEG C of high temperature, can be grown in the acidic environment of 2.0 or more pH value, bile tolerance ability is strong, has the function of mould mycin of degrading simultaneously, bacillus pumilus 13824 is made after microbial inoculum to be used for feeding animals effect safe and reliable, is promoting nutrient digestion to absorb, is reducing diarrhea rate, feed efficiency is improved, growth aspect is promoted to have positive effect.
Description
Technical field
The invention belongs to microbiologies and feeding field of probiotic bacteria, specifically, being related to a kind of with bacteriostasis, resistance to height
Warm, acidproof, bile tolerance feeding bacillus pumilus and its application.
Background technique
Currently, food-safety problem is just causing more and more to pay close attention to.In many reasons for causing food-safety problem,
Important reason first is that using antibiotic as growth accelerator in feed.For normal bacteriosis, antibiotic is still
It is primary treatment means；But in terms of promoting growth of animal, then other green additives is needed to gradually replace antibiotic
Effect.So screening can effectively inhibit the pathogenic microorganisms such as Escherichia coli, salmonella, and animal productiong can be improved simultaneously
The bacillus of energy, and the microecological bacillus preparation with the application stability such as feed processing, storage and transportation is developed, have scientific
With the double meaning of reality.
The Tiny ecosystem industry development of China starts from last century late nineteen eighties, and very fast growth, mesh were entered at 2000 or so
It is preceding, the white-hot stage of market development and product competition is progressed into, there are more and more enterprises both at home and abroad to start to set foot in this
Field.And it is also anxious wait hasten one's steps in the technological development in probiotics field.Feeding microecological bacillus preparation product exists
Feed bio-additive is much in the market, but the good and bad jumbled together；The strain prominent function and apparent product of application effect is less
See.
Tiny ecosystem product presently, there are the problem of have: 1) stability of certain lactic acid bacteria class probiotic products is still under room temperature
One insoluble problem, this causes the viable bacteria stability of product in shelf life not good enough；2) it takes rear probiotics and reaches intestines
The viable count in road is influenced by the degeneration-resistant property of gastric acid, intestinal juice and strain itself, and difference is very big between different strain；3) bacillus
It is proliferated efficiency etc. the sprouting of bacterium in animal body of passing by one's way, always exists dispute；4) physiological activity of probiotics has bacterial strain special
Property, up to now, bacterial strain only few in number is proved to have more apparent physiological activity, and these prebiotic spies
Property cannot exist simultaneously in same bacterial strain.In addition, although Tiny ecosystem has the row of the individual plants such as bacillus subtilis at present
Industry standard occurs, but lacks unified industry quality standard there are also many probiotics preparations, and different Tiny ecosystem enterprises exists in addition
There are bigger differences in terms of strain exploitation and management, production technology and quality control, product design and application scheme, so micro- life
State industry generally need further to standardize.
The research for obtaining the novel bacillus with probiotic properties is of great significance.Separation and screening anti-microbial pathogen and
Bacillus pumilus with probiotic effects can preferably be applied to feed addictive, to replace antibiotic.But short and small gemma
Bacillus is less as the research and application of probiotics preparation or feed addictive, especially in substitution feeding antibiotic and reduction ring
Border pollution etc..
Summary of the invention
The object of the present invention is to provide one kind have bacteriostasis, high temperature resistant, acidproof, bile tolerance and have degradation mould poison
The feeding bacillus pumilus of element effect and its application.
In order to achieve the object of the present invention, bacillus pumilus of the invention (Bacillus pumilus) is from Beijing
It screens in soil near certain pig raising Experimental Base and is obtained by ultraviolet light mutagenesis, be named as 13824.Through 16S rna gene sequence
Column analysis, the bacterial strain 13824 are bacillus pumilus (Bacillus pumilus).The bacterial strain was protected on September 27th, 2018
Ensconce China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC, address: Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101), classification naming is bacillus pumilus
(Bacillus pumilus), deposit number are CGMCC No.16539.
The Microbiological Characteristics of bacillus pumilus (Bacillus pumilus) 13824 are as follows: gram-positive bacteria, cell
Form be it is rod-shaped, of length no more than 3 μm, width is no more than 0.7 μm, and there is gemma and gemma not expand；Single colonie size is 10-
12mm, color are in faint yellow, opaque, bacterium colony surface folding, and edge is irregular.Thallus survival rate after 80 DEG C of processing 10min
Up to survival rate after 95% or more, 20min still up to 90% or more, can be grown in the acidic environment of 2.0 or more pH value, bile tolerance energy
Power is strong.And there is the ability of certain degradation mycotoxin, 9.75% is reached to the degradation rate of fumonisin, to vomitoxin
Degradation rate reach 7.33%.
The present invention provides the microbial inoculum containing the bacillus pumilus 13824.
The present invention also provides a kind of animal feed additives containing the bacillus pumilus 13824.The feed addictive
13824 viable count of bacillus pumilus contained is 1 × 108CFU/g~1 × 1012CFU/g；Preferably, the short and small gemma contained
The viable count of 13824 feed addictive of bacillus is 1 × 109CFU/g~1 × 1011CFU/g。
The present invention also provides a kind of animal feeds containing the bacillus pumilus 13824.Wherein, in the animal feed
The viable count of bacillus pumilus 13824 is 1 × 106CFU/kg~1 × 109CFU/kg, preferably 1 × 107CFU/kg~1 ×
108CFU/kg。
The present invention identifies the probiotic effects of bacillus pumilus 13824 by vitro method, the results showed that, short and small gemma bar
Bacterium 13824 can acidproof, sour cholate, the interior environment of gastrointestinal tract can be resisted, have the potentiality of probiotics.
It has also been found that ability of the bacillus pumilus 13824 with degradation mycotoxin, therefore the present invention provides
The drug of inhibition mycotoxin containing bacillus pumilus 13824, and provide bacillus pumilus 13824 and eaten in reduction
Application in product or Mycotoxins in Feed pollution.In the embodiment of the present invention, 13824 pairs of volt horse poison of bacillus pumilus are demonstrated
The degradation rate of element reaches 9.75%, reaches 7.33% to the degradation rate of vomitoxin.Those skilled in the art being capable of gene this hair
The bacillus pumilus 13824 of bright offer, for verifying its degradation effect to other mycotoxins, this is without departing from this field skill
The basic capacity range of art personnel, therefore bacillus pumilus 13824 has degradation mycotoxin and is used to prepare degradation mould
The drug of toxin and the application in reduction food or Mycotoxins in Feed pollution all belong to the scope of protection of the present invention.
Feeding bacillus pumilus of the present invention, since gemma structure is bad to drying, high temperature, high pressure, oxidation etc.
Environmental resistance is very strong, and this stability increases its potentiality as probiotics.The present invention further demonstrates short and small gemma
Application effect of the bacillus 13824 in weanling pig feed addition, it is found that the bacterial strain has reduces the raising of diarrhea of weaned piglets rate
The effect of feed conversion rate.Show that bacillus pumilus 13824 can be used as a kind of novel probiotic additive, is widely used in
In feed.The bacillus pumilus 13824 that the present invention screens, which has, improves efficiency of feed utilization, promotes nutriment in feed
Digestion and absorption；Enhance the immune function of animal, improve daily gain, reduces feedstuff-meat ratio；It is pollution-free, noresidue, biological environmental production etc.
Feature has significant effect in terms of promoting growth of animal and improving the weight of animals.
Detailed description of the invention
Fig. 1 is the colonial morphology figure of bacillus pumilus (Bacillus pumilus) 13824CGMCC No.16539.
Fig. 2 is the Gram's staining figure of bacillus pumilus (Bacillus pumilus) 13824CGMCC No.16539.
The acid resistance that Fig. 3 is bacillus pumilus (Bacillus pumilus) 13824CGMCC No.16539 detects knot
Fruit.
The bile tolerance that Fig. 4 is bacillus pumilus (Bacillus pumilus) 13824CGMCC No.16539 detects knot
Fruit.
Fig. 5 is the growth curve of bacillus pumilus (Bacillus pumilus) 13824CGMCC No.16539.
Fig. 6 is to carry out mouse filling using bacillus pumilus (Bacillus pumilus) 13824CGMCC No.16539
Stomach excess test after Histological Study figure.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means.Material as used in the following examples, examination
Agent etc. is commercially available unless otherwise specified.
Culture medium as used in the following examples is formulated as follows unless otherwise specified: LB culture medium: tryptone 10g,
Yeast extract 5g, sodium chloride 10g are settled to 1L with distilled water, and pH is adjusted to 7.0 with the sodium hydroxide of 5mol/L.
The separation and identification of 1 bacillus pumilus of embodiment (Bacillus pumilus) 13824
One, the separation of bacterial strain 13824
1. bacterial strain is separately cultured
The pedotheque near the Haidian District, Beijing City 1g China Agricultural University western school district pig raising base is taken to be incorporated with 9ml physiology
In the test tube of salt water, vortex device concussion is mixed, as 1:10 dilution, then is taken dilution to carry out 10 times and be incremented by dilution, is then selected
Each 1ml of dilution for selecting 3 suitable gradients is coated on LB culture medium.37 DEG C culture 48-72 hours, observe and record bacterium colony shape
State, the single colonie that picking grows fine carry out scribing line and isolate and purify.
2. ultraviolet mutagenesis and the screening of bacterial strain
LB culture medium after sterilizing is poured into culture dish, the resulting bacteria suspension of step 1 is taken to be coated on plate after to be solidified
On, each culture dish control bacterium colony is at 50 or so, after culture 12 hours, apart from ultraviolet lamp 20cm, mutagenesis 30s.
Bacterial strain after picking mutagenesis, is inoculated in LB liquid medium, cultivates 24 hours, measures OD value, chooses growth speed
Faster bacterial strain is spent, suitable bacteria suspension is taken to be coated on LB plate, culture was carried out to 24 hours in 37 DEG C of constant incubators
Next step Gram's staining.
3. the Gram's staining of bacterial strain
Sterilized distilled water is dripped on glass slide, the faster single colonie (bacterium of the speed of growth after one mutagenesis of picking
Fall aspect graph and see Fig. 1) it dissolves in water, after scraping blade, dries and fix on alcolhol burner.Violet staining liquid is added dropwise, contaminates 2min,
Washing, naturally dry；Iodine solution mordant dyeing 2min, washing, naturally dry is added dropwise；Basic fuchsin ethanol solution 50S is added dropwise, washes, from
So dry；It is observed on ordinary optical microscope, if thallus is purple for the positive, thallus takes on a red color as feminine gender, as a result sees Fig. 2.
It chooses Gram-positive bacillus and carries out next step spore staining experiment.
4. the spore staining of bacterial strain
The faster bacterial strain of the speed of growth after step 2 of learning from else's experience mutagenesis, drips sterilized distilled water, picking on glass slide
One single colonie dissolves in water, after scraping blade, dries and fixes on alcolhol burner.5% malachite green solution 3-5 drop is added dropwise,
3-5min is heated on alcolhol burner, pays attention to that liquid boiling or withered, washing, naturally dry cannot be made；The dyeing of Huang red solution is added dropwise
2min, washing, naturally dry；It is observed under ordinary optical microscope, gemma takes on a red color in green, thallus.
By the separation screening of step 1-4, one plant of Gram-positive is finally obtained, there is gemma and gemma does not expand
Bacterial strain.It is 13824 by the strain number.
Two, the identification of bacterial strain 13824
1. Morphological Identification
To in logarithmic growth phase and the stable bacterial strain 13824 of bacterium colony size carries out single colonie state description, mainly include
Size, color, transparency, bacterium colony surface state and the colony edge state of bacterium colony.Gained single colonie size is 10-12mm, face
Color is in faint yellow, opaque, bacterium colony surface folding, and edge is irregular.
Secondly the bacterial strain 13824 in logarithmic growth phase is dyed, using optical microphotograph sem observation thalli morphology.Point
From and the bacterial strain 13824 that screens, Gram's staining is positive, cellular morphology be it is rod-shaped, diameter is no more than 1 μm, has gemma raw
At and gemma do not expand.
2.16S RNA sequence homology analysis
The extraction of bacteria total DNA is extracted using the bacterial genomes DNA extraction kit of Tiangeng biochemical technology Co., Ltd.
Sample after extraction is sent to Shanghai Major Biological Medical Technology Co., Ltd. and is sequenced.By measurement result in GenBank data
BLAST sequence analysis is carried out in library, determines that strain classification is bacillus pumilus (Bacillus pumilus).
It is above-mentioned the experimental results showed that, the bacterium be bacillus pumilus.The bacterial strain was preserved in China on September 27th, 2018
Microbiological Culture Collection administration committee common micro-organisms center (abbreviation CGMCC, address: BeiChen West Road, Chaoyang District, BeiJing City 1
Institute 3, Institute of Microorganism, Academia Sinica, postcode 100101), classification naming is bacillus pumilus (Bacillus
Pumilus), deposit number is CGMCC No.16539.
The resistance of 2 bacillus pumilus 13824 of embodiment detects
1. heat resistance detects
By (CGMCC No.16539) bacterium solution of bacillus pumilus 13824 as 20 minutes in water-bath, respectively with 60 DEG C,
80 DEG C, 100 DEG C of processing, 3 repetitions of each processing, after treatment measure its viable count using tilt-pour process.
Bacillus pumilus 13824CGMCC No.16539 is handled after twenty minutes at 60 DEG C, and survival rate is up to 99%, 80 DEG C
Processing still can reach 80% or more up to 90% or more, 100 DEG C of processing after twenty minutes after twenty minutes.
2. acid resistance detects
Bacillus pumilus 13824 is inoculated into respectively in the LB culture medium that pH value is 2.0,3.0,4.0,5.0,6.0, point
Its viable count is not measured using plate tilt-pour process in 1h, 2h, 3h, 4h.
When being 5.0 or 6.0 in weak acid environment, that is, pH, bacillus pumilus can be with normal growth, when pH is 4.0
When, slightly have inhibiting effect to the growth of bacillus pumilus, when pH be 2.0 or 3.0 when, have when viable count is compared with pH5.0 or 6.0 compared with
Big decline, but still 1 × 10 can be maintained6CFU/ml.As a result see Fig. 3, when pH is 2, after inoculation viable bacteria 4 hours, viable bacteria
It is consistent substantially when quantity is with inoculation.Illustrate that the strain is extremely strong to the tolerance of acid.As a result bacillus pumilus is prompted
13824 can be resistant to the influence of gastric acid.
3. bile tolerance detects
Activated bacillus pumilus 13824CGMCC No.16539 is done into doubling dilution with sterile saline, is selected
It takes suitable dilution gradient and draws 1ml dilution and be put in sterile petri dish, do 6 repetitions, then use ox containing various concentration
The LB culture medium pour plate of sodium taurocholate (0.1%, 0.2%, 0.3%, 0.4%), 37 DEG C were cultivated 4 hours, every 1 hour bacterium colony
Count, at the same be free of natrii tauroglycocholas LB culture medium pour plate, 37 DEG C cultivate 48 hours, bacterium colony counting, as a control group,
See Fig. 4, as the result is shown the viable count under different gallbladder salinities with the extension of time it is generally on a declining curve.0.1% cholate
The influence acted on to bacillus pumilus 13824 is fainter, has little influence on its normal growth.In 0.2% and 0.3% gallbladder
After salt action 4 hours, viable count is average still to may remain in 5lg cfu/ml, living behind cholate effect 4 hours of 0.4%
Bacterium number average out to 4.7lg cfu/ml, bacillus pumilus 13824 more than half survive under 0.4% cholate, illustrate short
The bile tolerance ability of bacillus pumilus 13824 is stronger.
4. antibiotics sensitivity detects
The bacillus pumilus 13824CGMCC No.16539 of suitable concentration is coated on LB culture medium, each culture
4 drug sensitive test papers are uniformly attached in ware, are cultivated 36 hours, and inhibition zone size is observed.
13824 pairs of 1 bacillus pumilus of table different antibiotics sensitivity results
| Title | Antibacterial circle diameter (mm) | Susceptibility |
| Tetracycline | 10-14 | In it is quick |
| Streptomysin | 15-20 | Gao Min |
| Penicillin | 15-20 | Gao Min |
| Gentamicin | >20 | It is extremely quick |
| Erythromycin | >21 | It is extremely quick |
| Cefradine | >22 | It is extremely quick |
| Amoxicillin | >23 | It is extremely quick |
| Chloramphenicol | >24 | It is extremely quick |
| Florfenicol | >25 | It is extremely quick |
This result shows that, bacillus pumilus 13824CGMCC No.16539 simultaneously do not have good drug resistance, therefore
It is safe and reliable for using as feeding probiotics.
5. bacillus pumilus 13824 tests the degradation effect of mycotoxin
Bacillus pumilus is seeded in 2ml contains 40 μ g/ml vomitoxins and 2ml contains 40 μ g/ml volt horse poison respectively
It in the fluid nutrient medium of element, is cultivated 3 days under control environment, expands concentration and carry out repeating test to 60 μ g/ml, using height after 3 days
Effect liquid phase chromatogram method detects the concentration of vomitoxin and fumonisin in culture medium, different degrees of decline occurs, shows
Bacillus pumilus 13824 has the ability of degradation mycotoxin.Its to the degradation rate of fumonisin up to 9.75%, to vomiting poison
The degradation rate of element is up to 7.33%.The calculation of degradation rate is (mycotoxin additive amount-mycotoxin residual quantity)/mycotoxin
Additive amount × 100%.
The growth curve of 3 bacillus pumilus 13824 of embodiment measures
Growth curve represents the bacterium dynamic of growth and breeding up to aging death overall process in new suitable environment
Variation.The inoculum concentration that bacillus pumilus 13824CGMCC No.16539 is pressed to 10% (v/v), is inoculated into LB culture medium, 37
DEG C culture 46 hours, the LB culture medium of bacterium solution is not added as blank control, every 2-6 hours measurement OD600 values, to calculate
Viable count.Experiment is set to be repeated three times, as a result takes its average value, is recorded data and is drawn growth curve.As shown in figure 5, in 0-22
Hour, bacillus pumilus 13824 is in logarithm growth stage, and proliferative speed is higher.In 22-46 hours bacillus pumilus
13824 numbers tend towards stability.Enter the growth rate decline stage after 46 hours.
The preparation of 4 Bacillus pumilus preparation of embodiment
1. fermentative medium formula: brown sugar 40g/L, dregs of beans 35g/L, sodium chloride 4g/L, potassium dihydrogen phosphate 0.8g/L, sulfuric acid
Manganese 0.3g/L, magnesium sulfate 0.03g/L, defoaming agent 0.05% (v/v) plus water sufficiently dissolve, and control pH in the range of 6.6-6.9
Fermentation medium is made.
2.121 DEG C high-temperature steam sterilization 30min.
3. being inoculated with 24 hours bacterium solutions of cell age 5% (v/v) when fermentation medium is cooled to 30 DEG C.
4. keeping revolving speed 250rpm stirring under the conditions of 37 DEG C, fermented and cultured 20 hours, putting tank, obtain short and small gemma bar
The viable count of bacterium is greater than 2.0 × 109Cfu/ml, gemma rate is up to 95% or more.
5. bacterium mud to be put into low-temperature vacuum drying case to dry, sieving, collection product obtains Bacillus pumilus preparation.
Safety evaluatio the present embodiment of 5 bacillus pumilus 13824CGMCC No.16539 preparation of embodiment is with mouse
As experimental animal, the method tested using stomach-filling evaluates the safety of bacillus pumilus, the specific method is as follows:
1. the freeze-dried powder of bacillus pumilus microbial inoculum made from 4 method of embodiment, measures through plate count, short and small gemma
13824 bacterium number of bacillus is 1.9 × 109cfu/g。
2. choosing mouse 72 of 8 week old or so, being randomly divided into 4 groups, (A group is that control group gavages sterile saline, B group
It is high dose group according to 1.9 × 109The amount of cfu/ only gavages bacterium solution, and C group is middle dose group according to 1 × 108The amount of cfu/ only gavages
Bacterium solution, D group are low dose group according to 1 × 107The amount of cfu/ only gavages bacterium solution), every group of 3 repetitions, 6 mouse of each repetition.
3. every morning, 9 stomach-fillings were primary, continuously gavage 21 days.
Control temperature humidity in experimental mouse room is constant, natural lighting, and mouse is freely eaten, drinks water, every 7 days cleaning mouse cages one
It is secondary.In experimentation, the state of mouse is observed and recorded daily, survival condition, whether there is or not clinical abnormal symptoms etc..
Testing index:
(1) terminate the same day by the way of heart extracting blood in experiment, experiment mice blood sample is obtained, after static centrifugation
Serum is obtained, it is solid for detecting Human Serum Albumin, total protein, high-density lipoprotein, low-density lipoprotein, triglycerides, gallbladder
The blood parameters such as alcohol, urea, tumor necrosis factor alpha.
(2) it takes the complete heart, liver,spleen,kidney (bilateral) to claim weight in wet base, calculates separately cardiac index=(wet heart weight/weight)
× 100%, liver index=(liver wet weights/weight) × 100%, index and spleen index=(spleen weight in wet base/weight) × 100%, kidney
Dirty index=(kidney weight in wet base/weight) × 100%.
(3) take the jejunum of each group experimental mouse, ileum, colon, liver, kidney, lung tissue solid in 4% paraformaldehyde
It is fixed, be dehydrated, embed and etc. slice is made, by the variation of the method observation form of hematoxylin eosin stain, as a result see figure
6。
2 different disposal group mouse survival situation of table
| A group | B group | C group | D group | |
| 7 days | Survival | Survival | Survival | Survival |
| 14 days | Survival | Survival | Survival | Survival |
| 21 days | Survival | Survival | Survival | Survival |
From table 2 it can be seen that using bacillus pumilus 13824CGMCC No.16539 to after intragastric administration on mice 21 days, it is real
It tests each processing group mouse to survive, illustrates above-mentioned bacillus pumilus to animal safety.
The organ coefficient of 3 different disposal group mouse of table
| A group | B group | C group | D group | |
| Heart | 0.62 | 0.61 | 0.63 | 0.65 |
| Liver | 5.52 | 5.58 | 5.57 | 5.49 |
| Spleen | 0.41 | 0.41 | 0.42 | 0.44 |
| Kidney | 1.34 | 1.32 | 1.35 | 1.37 |
From table 3 it can be seen that the organ index of processing group mouse illustrates above-mentioned short compared with the control group without significant change
Bacillus pumilus does not cause Organs of Mice abnormal.
Using Biochemical Analyzer detection mice serum in albumin, total protein, high-density lipoprotein, low-density lipoprotein,
Triglycerides, cholesterol, urea, tumor necrosis factor alpha etc., as a result display is normal, illustrates that the embodiment of the present invention 4 provides
Bacillus pumilus preparation influence is not constituted on the physical signs of mouse.
The application of 6 bacillus pumilus 13824CGMCC No.16539 preparation of embodiment
28 age in days Ternary Pig three way cross weanling pig 72 is chosen in this experiment, experiment periods 45 days, sets according to random district's groups
Score is 2 groups, every group of 6 repetitions, 6 pigs of each repetition.A group is control group (basal diet group), and B group is processing group (base
Bacillus pumilus preparation made from the embodiment 4 of 240g/t is added in plinth daily ration, living bacteria count is 2.0 × 109cfu/g)。
In totally enclosed type conservation pigsty, temperature is controlled at 25-27 DEG C feeding piglet during test, is freely eaten, drinking-water.
Any antibiotic is free of in basal diet, piglet immunological is carried out according to routine immunization program.
Testing index: the production performance of each processing group weanling pig specifically includes following index:
1. the feed intake of record piglet daily, calculates average daily gain after the test；
2. recording pig weight on the day of on-test and end, average daily gain is calculated；
3. calculating feedstuff-meat ratio by the test result of a, b, calculation is average daily gain/average daily gain.
4. every morning, 10:00 observed and recorded the faecal condition of piglet during test, diarrhea of weaned piglets rate, abdomen are calculated
Rush down rate (%)=(diarrhea head number × Diarrhoea days)/(pig number × test number of days) × 100%.
Influence of the Bacillus pumilus preparation to Production Performance of Weaning Pigs and diarrhea rate is added in 4 basal diet of table
| Average daily gain (kg) | Average daily gain (kg) | Feedstuff-meat ratio | Diarrhea rate (%) | |
| A group | 0.489 | 0.342 | 1.430 | 2.1 |
| B group | 0.482 | 0.367 | 1.313 | 0.9 |
From table 4, it can be seen that processing group piglet average daily gain and average daily gain be all significantly higher than control group (P <
0.05), feedstuff-meat ratio is substantially less than control group (P < 0.05), illustrates that the feed efficiency for adding Bacillus pumilus preparation is more preferable.It raises
The grice diarrhoea rate that Bacillus pumilus preparation is added in material significantly reduces (P < 0.05), and illustrating that microbial inoculum of the invention has reduces
The effect of diarrhea of weaned piglets rate.
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be modified or is improved, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (10)
1. bacillus pumilus (Bacillus pumilus) 13824, deposit number is CGMCC NO.16539.
2. containing the microbial inoculum of bacillus pumilus described in claim 1 (Bacillus pumilus) 13824.
3. the feed addictive containing bacillus pumilus described in claim 1 (Bacillus pumilus) 13824 is dynamic
Object feed.
4. containing the drug of bacillus pumilus described in claim 1 (Bacillus pumilus) 13824.
5. drug as claimed in claim 4, which is characterized in that it is the drug for inhibiting mycotoxin.
6. drug as claimed in claim 5, it is characterised in that the mycotoxin is fumonisin, vomitoxin.
7. bacillus pumilus (Bacillus pumilus) 13824 described in claim 1 is mould in reducing food or feed
Application in verticillium toxin pollution.
8. bacillus pumilus (Bacillus pumilus) 13824 described in claim 1 is in preparing feed addictive
Using.
9. bacillus pumilus (Bacillus pumilus) 13824 described in claim 1 is in improving food conversion ratio
Using.
10. bacillus pumilus (Bacillus pumilus) 13824 described in claim 1 is promoting growth of animal or raising
Application in terms of the weight of animals.
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