CN109517739A - Double organs are coupled three-layer laminated integrated chip - Google Patents
Double organs are coupled three-layer laminated integrated chip Download PDFInfo
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- CN109517739A CN109517739A CN201811609624.8A CN201811609624A CN109517739A CN 109517739 A CN109517739 A CN 109517739A CN 201811609624 A CN201811609624 A CN 201811609624A CN 109517739 A CN109517739 A CN 109517739A
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- 210000000056 organ Anatomy 0.000 title claims abstract description 32
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 27
- 229920005573 silicon-containing polymer Polymers 0.000 claims abstract description 27
- 239000004926 polymethyl methacrylate Substances 0.000 claims abstract description 26
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims abstract description 24
- -1 methylsiloxane Chemical class 0.000 claims description 4
- BAPJBEWLBFYGME-UHFFFAOYSA-N Methyl acrylate Chemical compound COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 claims 2
- 230000008676 import Effects 0.000 claims 1
- 239000000090 biomarker Substances 0.000 abstract description 4
- 238000013461 design Methods 0.000 abstract description 4
- 238000004445 quantitative analysis Methods 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 230000035479 physiological effects, processes and functions Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 238000003255 drug test Methods 0.000 description 3
- 150000004702 methyl esters Chemical class 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000019522 cellular metabolic process Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000000017 hydrogel Substances 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 208000011231 Crohn disease Diseases 0.000 description 1
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 206010070863 Toxicity to various agents Diseases 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000012362 drug development process Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001973 epigenetic effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 229920005570 flexible polymer Polymers 0.000 description 1
- 238000010362 genome editing Methods 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000002894 multi-fate stem cell Anatomy 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
- 230000003372 organotropic effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61H—PHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
- A61H1/00—Apparatus for passive exercising; Vibrating apparatus; Chiropractic devices, e.g. body impacting devices, external devices for briefly extending or aligning unbroken bones
- A61H1/02—Stretching or bending or torsioning apparatus for exercising
- A61H1/0237—Stretching or bending or torsioning apparatus for exercising for the lower limbs
- A61H1/0266—Foot
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61H—PHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
- A61H9/00—Pneumatic or hydraulic massage
- A61H9/005—Pneumatic massage
- A61H9/0078—Pneumatic massage with intermittent or alternately inflated bladders or cuffs
-
- A—HUMAN NECESSITIES
- A63—SPORTS; GAMES; AMUSEMENTS
- A63B—APPARATUS FOR PHYSICAL TRAINING, GYMNASTICS, SWIMMING, CLIMBING, OR FENCING; BALL GAMES; TRAINING EQUIPMENT
- A63B23/00—Exercising apparatus specially adapted for particular parts of the body
- A63B23/035—Exercising apparatus specially adapted for particular parts of the body for limbs, i.e. upper or lower limbs, e.g. simultaneously
- A63B23/04—Exercising apparatus specially adapted for particular parts of the body for limbs, i.e. upper or lower limbs, e.g. simultaneously for lower limbs
- A63B23/08—Exercising apparatus specially adapted for particular parts of the body for limbs, i.e. upper or lower limbs, e.g. simultaneously for lower limbs for ankle joints
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61H—PHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
- A61H2201/00—Characteristics of apparatus not provided for in the preceding codes
- A61H2201/16—Physical interface with patient
- A61H2201/1602—Physical interface with patient kind of interface, e.g. head rest, knee support or lumbar support
- A61H2201/164—Feet or leg, e.g. pedal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61H—PHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
- A61H2205/00—Devices for specific parts of the body
- A61H2205/12—Feet
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Abstract
The invention discloses a kind of double organs to be coupled three-layer laminated integrated chip, including the first polymethyl methacrylate layers, the first dimethyl silicone polymer layer, the second dimethyl silicone polymer layer and the second polymethyl methacrylate layers from top to bottom set gradually, it is spaced apart and is set there are two miniature culture pond on the binding face of the first dimethyl silicone polymer layer and the second dimethyl silicone polymer layer, each culture pond is provided with the inlet tube and outlet extended in culture pond by the first polymethyl methacrylate layers upper surface.The present invention is spaced apart the cell set there are two two kinds of Different Organs of culture pond culture on the binding face of the first dimethyl silicone polymer layer and the second dimethyl silicone polymer layer, according to the Quality Design culture solution type and flow velocity of micro-organs, micro-organs cell morphology and survival rate, and the content of quantitative analysis each group organ biomarker can directly be observed.
Description
Technical field
The invention belongs to cultivate organ chip field, more particularly to a kind of double three-layer laminated integrated cores of organ coupling
Piece.
Background technique
The scientist headed by WYSS research institute, Harvard University proposes the concept of organ chip in recent years, it would be desirable to enough
Microchip replicates the function of human organ, even replaces controversial zoopery to reduce in drug development process,
Clinical Transformation Application is set to become the more easy idealization diagnosis ex vivo that can also be achieved disease.Organ chip is by calculator memory
The transparent flexible polymer of size is constituted, and inside is hollow microfluidic channel, the human cell of channel liner work.Study people
Member goes out the physiology and mechanical function of human organ by design simulation, observes the response situation of cell in real time in drug test.
The chip developed includes lung, heart, liver, kidney, blood vessel and marrow etc..
Scientist in clinical precisely diagnosis and accurate therapeutic process using more predictability, more effective means for being come
The exploration for carrying out Disease Warning Mechanism and effective and safe medication never stopped.Mainly carried out at present using experimental animal and cell model
The research of above-mentioned problem in science.However, the nonuniformity and autoblast model microenvironment of human body and animal epigenetic without
Method simulation target is organotropic in body state, while the limitation of ethics, these are all that disease diagnosis and therapy research instantly is difficult to
The realistic problem and challenge of avoidance seriously constrain our scientific knowledges to human diseases, are badly in need of the appearance of new model.
To solve this problem, the organ that can only be implanted into single type in the single organ chip developed at present mostly is thin
Born of the same parents, and really life process is multiple organ synergistic effects as a result, therefore, ideal way is by various organ chipsets
It is merged the manikin for connecing one micromation of building, helps us understand and recognize the physiology and pathologic process of human body.In human body
It is different with the active function micro-organs biochemical parameter of physiological metabolism.And during body metabolism and drug test, often
It often needs while monitoring the double organ metabolic conditions connected each other as a whole.Therefore, how according to reflection Human Physiology dissection
The physiology pharmacokinetic model (physiologically based pharmacokinetics, PBPK) of structure establishes more devices
Official is coupled chip, the dynamic change of more preferable simulation human body generated metabolite when being changed by outside stimulus or internal gene
Change, becomes the emphasis of those skilled in the art's research.
Summary of the invention
It is an object of the invention to provide a kind of double organs to be coupled three-layer laminated integrated chip, can be fully solved above-mentioned existing
There is the shortcoming of technology.
The purpose of the present invention is realized by following technical proposals:
The three-layer laminated integrated chip of a kind of double organ couplings, including the first polymethyl from top to bottom set gradually
Sour methyl esters layer, the first dimethyl silicone polymer layer, the second dimethyl silicone polymer layer and the second polymethyl methacrylate layers, institute
It states to be spaced apart on the binding face of the first dimethyl silicone polymer layer and the second dimethyl silicone polymer layer and set there are two culture pond, often
A culture pond is provided with the inlet tube and outlet extended in culture pond by the first polymethyl methacrylate layers upper surface.
Preferably, the inlet tube extends to the left bottom of culture pond, the outlet extends to the right side of culture pond
Lateral roof.
Preferably, being arranged in the second polymethyl methacrylate layers, there are two the circular holes that diameter is 16mm-18mm, each
One culture pond of circular hole face is easy to use two culture ponds of micro- sem observation by circular hole.
Preferably, each culture pond includes the first culture for being opened in the first dimethyl silicone polymer layer lower surface
Slot and the second culture tank for being opened in the second dimethyl silicone polymer layer upper surface, first culture tank are overlapped in the second culture
Slot upper surface.
Preferably, first culture tank and the second culture tank are cylindrical structure, it is highly 1mm-2mm, diameter is
10mm-12mm。
Compared with prior art, the beneficial effects of the present invention are:
The configuration of the present invention is simple, design is reasonable, in the first dimethyl silicone polymer layer and the second dimethyl silicone polymer layer
Binding face on be spaced apart the cell set there are two two kinds of Different Organs of culture pond culture, according to the Quality Design culture of micro-organs
Flow velocity can directly observe micro-organs cell morphology and survival rate, the content of quantitative analysis each group organ biomarker.
Detailed description of the invention
Fig. 1 is longitudinal section view of the invention；
Fig. 2 is exploded view of the invention.
Appended drawing reference: the first polymethyl methacrylate layers of 1-, 2- the first dimethyl silicone polymer layer, the poly- diformazan of 3- second
Radical siloxane layer, the second polymethyl methacrylate layers of 4-, the first screw of 5-, the first lock nut of 6-, the second screw of 7-, 8-
Two lock nuts, the first culture tank of 9-, the second culture tank of 10-, 11- inlet tube, 12- outlet, 13- circular hole, 14- third spiral shell
Nail, 15- third lock nut.
Specific embodiment
The present invention is further illustrated with attached drawing combined with specific embodiments below.
As shown in Figure 1 to Figure 2, a kind of double organs are coupled three-layer laminated integrated chip, including are from top to bottom successively bonded
The first polymethyl methacrylate layers 1, the first dimethyl silicone polymer layer 2, the second dimethyl silicone polymer layer 3 and second poly-
The length of methyl methacrylate layer 4, the first dimethyl silicone polymer layer 2 and the second dimethyl silicone polymer layer 3 is 75mm-
80mm, width are 25mm-30mm, are highly 4mm-6mm, the first polymethyl methacrylate layers 1 and the second poly- methyl-prop
The length of e pioic acid methyl ester layer 4 is 86mm-90mm, and width is 35mm-40mm, is highly 3mm-5mm, the first poly- methyl-prop
The left and right ends of e pioic acid methyl ester layer 1 and the left and right ends of the second polymethyl methacrylate layers 4 offer threaded hole, use
One screw 5 is threadedly coupled the left end of the first polymethyl methacrylate layers 1 and the left end of the second polymethyl methacrylate layers 4,
And locked with the first lock nut 6, the right end and the of the first polymethyl methacrylate layers 1 is threadedly coupled using the second screw 7
The right end of two polymethyl methacrylate layers 4, and locked with the second lock nut 8, similarly, in the first polymethyl methacrylate
The front of and behind of layer 1 and the second polymethyl methacrylate layers 4 is solid by third screw 14 and the locking of third lock nut 15
Fixed first polymethyl methacrylate layers 1 and the second polymethyl methacrylate layers 4, four layer materials of such chip pass through screw
It fits and is fixed together, form closed double organ chip sets, be similar to sandwich sandwich structure, the first polydimethylsiloxanes
Culture pond there are two setting, the second poly-methyl methacrylate are spaced apart on the binding face of alkane layer 2 and the second dimethyl silicone polymer layer 3
The circular hole 13 that diameter is 16mm-18mm is provided in ester layer 4, each 13 face of circular hole culture pond one by one can not beaten in this way
Microscope direct observing micro-organs upgrowth situation in the case where minisize reaction pond is opened, each culture pond includes being opened in first poly- two
First culture tank 9 of 2 lower surface of methylsiloxane layer and the second culture for being opened in 3 upper surface of the second dimethyl silicone polymer layer
Slot 10, the first culture tank 9 are overlapped in 10 upper surface of the second culture tank, and the first culture tank 9 and the second culture tank 10 are cylindrical knot
Structure is highly 1mm-2mm, and diameter 10mm-12mm, the right end of the first culture tank 9 is provided with and communicated with for placing outlet 12
Notch, the left end of the second culture tank 10 is provided with and communicated with the notch for placing inlet tube 11, and each culture pond is provided with
Inlet tube 11 and outlet 12 in culture pond are extended to by 1 upper surface of the first polymethyl methacrylate layers, inlet tube 11 prolongs
The bottom of notch on the left of culture pond is extended to, outlet 12 extends to the top of notch on the right side of culture pond.
The present invention uses stability, the robustness that polymethyl methacrylate (PMMA) sheathing material is due to this material
And the transparency, and the internal material that dimethyl silicone polymer (PDMS) material is used as organ chip is then due to material itself
Biocompatibility, elasticity and leakproofness, there are two miniature culture pond, culture ponds to be connected with extraneous microfluidic system for core inner setting
It is recyclable to pump out different classes of cell culture fluid to support the cell being embedded in GelMA hydrogel to grow and due to entirety
The transparency of chip can observe the growing state of three-dimensional micro-organs (liver micro-organs and kidney micro-organs) in real time.
Organ microchips of the invention double while comprising hydrogel cell culturing bracket with certain liquid crushing resistance,
Wherein special detail setting is but also the subsequent Biology Observation that will be carried out and biomarker analysis are more easy.Together
Sample can also supply nutrition required for two kinds of micro-organs by the culture fluid circulation of main support of microfluidic system in time
And biomarker and waste caused by cell metabolism are taken away by efferent duct in time.Therefore, the double organs of entire liver-kidney
Microchip, that is, analog human inner cell metabolism and nutriment exchange process.
With continuous research of the invention, the present invention has wide potential application foreground, other than drug screening, organ
Chip also has revolutionary potentiality in terms of the human test of chemicals, toxin and cosmetics etc..WYSS research institute grinds
Study carefully personnel and carries out toxin and Contaminant measurement using lung chip.
Meanwhile organ chip is also the new method that scientist imitates human diseases.Current many human diseases are not dynamic
Object model, such as Crohn disease.Its pathogenic factor is unknown so far, also lacks effective treatment means, maximum reason is just
It is that can not find to suffer from the animal of this disease equally to carry out drug test.And human mould is established using human body chip technology
Type, this problem are just readily solved.
In terms of personalized medicine, usable genome editing technique successfully prepares individual pathological tissues chip at present, examines
It examines and the effect that gene product corrects its defect is provided.Or patient is induced multi-potent stem cell into (iPS) and does specific cells induction point
Human body chip is implanted into after change, to construct personalized drug toxicity Evaluation Platform.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (5)
1. a kind of double organs are coupled three-layer laminated integrated chip, it is characterised in that: including from top to bottom set gradually first
Polymethyl methacrylate layers (1), the first dimethyl silicone polymer layer (2), the second dimethyl silicone polymer layer (3) and second are poly-
Methyl methacrylate layer (4), the patch of the first dimethyl silicone polymer layer (2) and the second dimethyl silicone polymer layer (3)
It is spaced apart and is set there are two culture pond on conjunction face, each culture pond is provided with by table on the first polymethyl methacrylate layers (2)
Face extends to inlet tube (11) and outlet (12) in culture pond.
2. a kind of double organs according to claim 1 are coupled three-layer laminated integrated chip, it is characterised in that: the import
Pipe (11) extends to the left bottom of culture pond, and the outlet (12) extends to the right hand top of culture pond.
3. a kind of double organs according to claim 1 are coupled three-layer laminated integrated chip, it is characterised in that: the second poly- first
It is arranged on base methyl acrylate layer (4) there are two the circular hole (13) that diameter is 16mm-18mm, each circular hole (13) face one is trained
Pond is supported, two culture ponds of micro- sem observation are easy to use by circular hole (13).
4. a kind of double organs according to claim 1 are coupled three-layer laminated integrated chip, it is characterised in that: described each
Culture pond includes being opened in first culture tank (9) of first dimethyl silicone polymer layer (2) lower surface and being opened in second poly- two
Second culture tank (10) of methylsiloxane layer (3) upper surface, first culture tank (9) are overlapped on the second culture tank (10)
Surface.
5. a kind of double organs according to claim 3 are coupled three-layer laminated integrated chip, it is characterised in that: described first
Culture tank (9) and the second culture tank (10) are cylindrical structure, are highly 1mm-2mm, diameter 10mm-12mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811609624.8A CN109517739A (en) | 2018-12-27 | 2018-12-27 | Double organs are coupled three-layer laminated integrated chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811609624.8A CN109517739A (en) | 2018-12-27 | 2018-12-27 | Double organs are coupled three-layer laminated integrated chip |
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| Publication Number | Publication Date |
|---|---|
| CN109517739A true CN109517739A (en) | 2019-03-26 |
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|---|---|---|---|
| CN201811609624.8A Pending CN109517739A (en) | 2018-12-27 | 2018-12-27 | Double organs are coupled three-layer laminated integrated chip |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150253309A1 (en) * | 2012-09-28 | 2015-09-10 | Tissuse Gmbh | Multi-organ chip with improved life time and homoeostasis |
| CN106811413A (en) * | 2015-11-30 | 2017-06-09 | 中国科学院大连化学物理研究所 | Multiple organ chip based on microflow control technique and preparation method thereof |
| WO2018094003A1 (en) * | 2016-11-16 | 2018-05-24 | President And Fellows Of Harvard College | Coupled organ-on-chip systems, devices, and methods of use for mimicking a de-coupled organ |
| CN209584263U (en) * | 2018-12-27 | 2019-11-05 | 重庆大学 | Double organs are coupled three-layer laminated integrated chip |
-
2018
- 2018-12-27 CN CN201811609624.8A patent/CN109517739A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150253309A1 (en) * | 2012-09-28 | 2015-09-10 | Tissuse Gmbh | Multi-organ chip with improved life time and homoeostasis |
| CN106811413A (en) * | 2015-11-30 | 2017-06-09 | 中国科学院大连化学物理研究所 | Multiple organ chip based on microflow control technique and preparation method thereof |
| WO2018094003A1 (en) * | 2016-11-16 | 2018-05-24 | President And Fellows Of Harvard College | Coupled organ-on-chip systems, devices, and methods of use for mimicking a de-coupled organ |
| CN209584263U (en) * | 2018-12-27 | 2019-11-05 | 重庆大学 | Double organs are coupled three-layer laminated integrated chip |
Non-Patent Citations (3)
| Title |
|---|
| LEILA CHOUCHA-SNOUBER等: "Investigation of Ifosfamide Nephrotoxicity Induced in a Liver–Kidney Co-Culture Biochip", 《BIOTECHNOLOGY AND BIOENGINEERING》, vol. 110, no. 2, pages 599 * |
| SANGEETA N BHATIA等: "Microfluidic organs-on-chips", 《NATURE BIOTECHNOLOGY》, vol. 32, no. 8, pages 765 * |
| 黄恒顺;邓九;李晓瑞;陈宗正;罗勇;: "微流控肠-肝-乳腺癌芯片的构建及其体外药物PK-PD分析", 中国实验方剂学杂志, no. 02 * |
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