CN109507431B - Device, machine and method for incubation washing treatment of biological sample membrane or glue - Google Patents
Device, machine and method for incubation washing treatment of biological sample membrane or glue Download PDFInfo
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- CN109507431B CN109507431B CN201811264588.6A CN201811264588A CN109507431B CN 109507431 B CN109507431 B CN 109507431B CN 201811264588 A CN201811264588 A CN 201811264588A CN 109507431 B CN109507431 B CN 109507431B
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Abstract
A device for incubation washing treatment of biological sample membrane or gel comprises a washing basket frame, a washing basket which can be arranged in the washing basket frame, a washing basket poking device which is used for poking the washing basket to rotate in the washing basket frame, and a washing tank which is used for placing the washing basket frame and incubation washing liquid; the basket washing shifter is assembled by combining the basket washing frame and the basket washing and then inserted into the washing tank to cooperate with the washing tank, and the shifting teeth or gears on the basket washing shifter pass through the opening of the basket washing frame on the wall of the basket washing frame to hook the opening of the basket washing; under the action of external downward pressure or a gear, the washing basket is driven to slide along the inner wall of the washing basket frame, the biological sample membrane or glue attached to the inner wall of the washing basket does irregular circular motion along with the motion of the washing basket, and repeatedly passes through incubation washing liquid at the bottom of the washing tank to finish the incubation washing process of the biological sample membrane; the dynamic roller is used for incubation and washing, the number of used antibodies is small, and cross contamination caused by human is effectively avoided; the structure is reliable, the cost is low, and the popularization is easy.
Description
Technical Field
The invention relates to the field of biological sample membrane or glue treatment, in particular to a device, a machine and a method for incubation washing treatment of biological sample membrane or glue.
Background
In biological experiments, membranes or gels carrying biological substances such as DNA, RNA, sugar, lipids, proteins, etc. are subjected to nucleic acid hybridization, antibody detection, staining, etc.; taking western blot detection as an example, there are processing steps of blocking, antibody incubation, washing and the like for a certain period of time for a membrane fixed with protein. In general terms, the membranes are placed in various, flowing liquids in a sequence, in time. And for these processing requirements; 1, uniformly incubating and washing; 2, using as few antibodies as possible; 3, cross contamination caused by manual irregular operation is avoided at one time.
The handling of the film as described above is usually done manually by a laboratory technician. It is foreseeable that the results are often unstable.
Various western blotting membrane processors are also available in the market, and even incubation and clean washing can be achieved although the requirements 1 can be met. But the requirements for the latter two points: 2, using as few antibodies as possible, 3, avoiding cross contamination at one time; are often unsatisfactory. Moreover, the disadvantage of high costs is also added. So that incubation washing of western blot membranes in the laboratory is still in the stage of manual completion 30 years after western blot testing invention.
Disclosure of Invention
The invention aims to solve the problems that various protein blotting membrane processors are available in the market, and even incubation and clean washing can be achieved; however, there are problems that the amount of antibody used is large, cross contamination is easy, and the cost is high.
The technical scheme adopted by the invention is as follows: a device for incubation washing treatment of biological sample membranes or gels comprises a washing basket frame, a washing basket capable of being installed in the washing basket frame, a washing basket poking device for poking the washing basket to rotate in the washing basket frame, and a washing tank for placing the washing basket and incubating washing liquid.
Furthermore, the front and rear walls of the washing basket frame are rectangular thin walls, the upper part of the front and rear walls is connected with a semicircular arc-shaped thin upper wall to form a smooth curved surface, the left and right ends of the front and rear walls are provided with oval frames, the lower end of the washing basket frame is open, and the walls are provided with a plurality of washing basket frame openings; the inner side of the basket washing frame is provided with a plurality of vertical and parallel strip-shaped bulges which are slightly higher than the wall surface.
Furthermore, the washing basket is a cylinder formed by rolling flexible sheet materials, the surface of the cylinder is provided with a washing basket opening for shifting the gear, and the inner surface of the cylinder is provided with punctiform or other forms of miniature protrusions.
Furthermore, the basket washing shifter is a basket washing shifting tooth shifting cover or a basket washing gear shifting cover; the washing basket shifting tooth shifting cover consists of a shell, a return spring and shifting teeth, after penetrating through the washing basket frame, the hook surface of the shifting teeth can hook and shift the washing basket, and the smooth surface can enable the washing basket to slide through the surface of the washing basket; the basket washing gear shifting cover shifts the basket through the rotating gear after penetrating through the basket washing frame.
Furthermore, the washing tank is of a cuboid structure with an open upper end, the inside of the washing tank is divided into a plurality of rectangular small tank bodies, the bottom of each small tank body is semi-cylindrical, and the radian of the cambered surface of each small tank body is consistent with that of the lower part of the washing basket frame; the bottom of the small trough body can be attached to the bottom of the washing trough or suspended, and the inner side surface of the bottom of the small trough body is provided with a point-shaped or strip-shaped bulge; the bottom of the small groove body can be hollowed out by a downward part.
The treatment method of the device for incubation washing treatment of the biological sample membrane or glue is characterized in that the device is assembled by a basket washing shifter, a basket washing frame and a basket washing are inserted into a washing tank and cooperated with the washing tank, and a shifting tooth or a gear on the basket washing shifter hooks the opening of the basket washing through the opening of the basket washing frame on the wall of the basket washing frame; under the action of external downward pressure or gears, the washing basket is driven to slide along the inner wall of the washing basket frame, the biological sample membrane or glue attached to the inner wall of the washing basket does irregular circular motion along with the motion of the washing basket, and repeatedly passes through incubation washing liquid at the bottom of the washing tank to finish the incubation washing process of the biological sample membrane.
Furthermore, the machine matched with the device for incubation washing treatment of the biological sample membrane or the glue comprises a control computer, a vertical displacement motor arranged at the top of the machine and a horizontal displacement motor arranged at the top of the machine, wherein the vertical displacement motor drives a basket washing poking device, a basket washing frame and a basket washing matching body to move up and down in a washing tank; the horizontal displacement motor drives the matching body to move horizontally on the washing tank, so as to complete the incubation and washing work of the biological sample membrane or glue; the control computer, the vertical displacement motor and the horizontal displacement motor can be replaced by the mechanical arm as a whole.
Furthermore, the machine matched with the device for incubation washing treatment of the biological sample membrane or the glue comprises a center column structure positioned in the middle of the washing tank and a cross beam erected on the center column structure through a 'n' -shaped connecting piece, wherein the cross beam drives a matching body consisting of a plurality of basket washing pokers, basket washing frames and basket washing to move up and down in the washing tank and move horizontally above the washing tank to finish the incubation washing work of the blotting membrane; the upper cover of the center post structure and the gap which is formed on the peripheral wall of the center post structure and is opened downwards can allow the 'n' -shaped connecting piece to freely move back and forth and up and down through the gap.
Furthermore, the machine matched with the device for incubation washing treatment of the biological sample membrane or glue comprises a machine square block capable of moving automatically, drives the washing combination to move up and down, back and forth and implements washing operation of the blotting membrane; the motor is used for driving the crawler to implement the front-back operation of the machine square block, and the motor is used for driving the upper part of the machine square block to implement the up-down motion; the complementary clamping groove and the convex strip are used for involution limiting of the upper part and the lower part of the square block of the machine; the cavity is used for accommodating the computer control part; the upper part of the machine square block is connected with a washing combination in a left-right mode, so that the washing combination can carry out washing operation of the blotting membrane and drive a matching body consisting of a basket washing shifter, a basket washing frame and a basket washing to move up and down in the washing tank and horizontally move above the washing tank, and incubation washing operation of the biological sample membrane or glue is carried out.
Further, the wash tank may be fitted with a container containing a plurality of pre-stored wash liquids; the pressure in the container changes, and the washing liquid enters and exits from the bottom of the washing tank through the pipeline and is used for incubation and washing.
Furthermore, a lining structure with the width consistent with that of the washing basket is arranged in the washing basket; the material is the same as that of the washing basket; the shape is cylindrical or irregular cylindrical close to the washing basket, but has no opening and the perimeter is smaller than that of the washing basket.
The invention has the advantages and characteristics that: 1, a dynamic roller is used for incubation and washing, the number of used antibodies is small, and 2, cross contamination caused by human is effectively avoided; 3 the structure is reliable, the cost is low, and the popularization is easy.
Drawings
FIG. 1 is a schematic diagram of an exploded structure of an apparatus for incubation and washing treatment according to a preferred embodiment of the present invention;
FIG. 2 is a schematic view of a basket according to a preferred embodiment of the present invention;
FIG. 3 is a schematic view of the structure of FIG. 2 from another angle;
FIG. 4 is an enlarged partial schematic view of a wash basket according to a preferred embodiment of the present invention;
FIG. 5 is a schematic structural view of a basket kicker according to a preferred embodiment of the present invention;
FIG. 6 is a schematic view of an alternate angle of the basket kicker according to the preferred embodiment of the present invention;
FIG. 7 is a schematic structural view of a wash tank according to a preferred embodiment of the present invention;
FIG. 8 is a schematic structural view of the bottom of the washer tub in accordance with the preferred embodiment of the present invention;
FIG. 9 is a schematic view of the structure of the bottom of another washer tub according to the preferred embodiment of the present invention;
FIG. 10 is a schematic view of the bottom of another wash tank according to the preferred embodiment of the present invention;
FIG. 11 is a schematic view of an alternative basket mover in accordance with the preferred embodiment of the present invention;
FIG. 12 is a schematic illustration of an incubation wash according to a preferred embodiment of the present invention;
FIG. 13 is a schematic illustration of a machine adapted to cooperate with the preferred embodiment of the present invention;
FIG. 14 is a schematic illustration of another machine configuration (robotic arm) incorporating the preferred embodiment of the present invention;
FIG. 15 is a schematic illustration of another machine configuration (robotic arm) incorporating the preferred embodiment of the present invention;
FIG. 16 is a schematic illustration of another machine configuration (center pillar configuration) incorporating the preferred embodiment of the present invention;
FIG. 17 is a schematic view of a "what is" type connection of the embodiment of FIG. 15;
FIG. 18 is a schematic block diagram of a machine block according to a preferred embodiment of the present invention;
FIG. 19 is a schematic diagram of the structure of the incubation and washing with the machine block of the preferred embodiment of the present invention;
FIG. 20 is a schematic view of a pre-stored washing liquid container installed in accordance with a preferred embodiment of the present invention;
FIG. 21 is a schematic structural view of the liner structure of the basket according to the preferred embodiment of the present invention.
The reference numbers in the figures denote: 1-basket washing frame, 1.1-semicircular arc-shaped thin upper wall, 1.2-frame, 1.3-basket washing frame opening, 1.4-strip-shaped protrusion, 2-basket washing, 2.1-basket washing opening, 2.2-micro protrusion, 2.3-lining structure, 3-basket washing shifter, 3.1-shell, 3.2-reset spring, 3.3-shifting tooth, 3.4-gear, 4-washing tank, 4.1-small tank body, 4.2-protrusion, 4.3-hollow, 5-vertical displacement motor, 6-horizontal displacement motor, 7-middle column structure, 7.1-gap, 8-cross beam, 9- 'several' shaped connecting piece, 10-machine block, 10.1-motor, 10.2-motor, 10.3-clamping groove, 10.4-raised strip, 10.5-cavity, 11-pre-storage washing liquid container, 12-a robotic arm;
Detailed Description
The invention is further illustrated with reference to the accompanying drawings:
referring to fig. 1, an apparatus for incubation washing treatment of biological sample membrane or gel comprises a basket 1, a basket 2 capable of being inserted into the basket, a basket shifter 3 (fig. 1 shows a basket shifting cover, and fig. 11 shows another basket gear shifting cover) for shifting the basket to rotate in the basket 1, and a washing tank 4 for placing the basket 1 and incubation washing liquid.
Referring to fig. 2 and 3, the front and rear walls of the basket 1 are rectangular thin walls, the upper part of the front and rear walls is connected with a semicircular arc-shaped thin upper wall 1.1 to form a smooth curved surface, the left and right ends of the front and rear walls are provided with elliptical frames 1.2, the lower end of the basket 1 is open, and the walls are provided with a plurality of basket openings 1.3; the inner side of the basket washing frame is provided with a plurality of vertical and parallel strip-shaped bulges 1.4 which are slightly higher than the wall surface, and the function of the basket washing frame is to avoid overhigh surface tension, so that the basket washing frame is attached to the wall of the basket washing frame, and the basket washing frame cannot be stirred.
Referring to fig. 4, the washing basket 2 is a cylinder formed by rolling a flexible sheet material, the surface of the cylinder is provided with a washing basket opening 2.1 for the poking of the poking teeth, the inner surface of the cylinder is provided with a punctiform or other forms of micro-protrusions 2.2,
please refer to fig. 5, 6 and 10, wherein the basket shifter 3 is a basket shifting gear shifting cover or a basket gear shifting cover; the washing basket shifting tooth shifting cover consists of a shell 3.1, a return spring 3.2 and shifting teeth 3.3, after penetrating through the washing basket frame, the hook surface of the shifting teeth can hook the shifting washing basket, and the smooth surface can enable the washing basket to slide through the surface of the shifting teeth; the basket washing gear shifting cover shifts the basket through the rotating gear 3.4 after penetrating through the basket washing frame.
Referring to fig. 7, fig. 8, fig. 9 and fig. 10, the washing tub 4 is a rectangular structure with an open upper end, the inside of the washing tub 4 is divided into a plurality of rectangular small slots 4.1, the bottom of the small slots 4.1 is semi-cylindrical, and the radian of the arc surface is consistent with the radian of the lower part of the basket washing frame 1; the bottom of the small groove body 4.1 can be attached to the bottom of the washing groove 4 or can be suspended, the inner side surface of the bottom of the small groove body 4.1 is provided with a bulge 4.2 (which can be a strip-shaped bulge, a point-shaped bulge or a brush-shaped bulge and has the function of avoiding overhigh surface tension so that the washing basket is attached to the wall of the washing basket frame and can not be stirred), and the inner side surface of the bottom of the small groove body 4.1 is provided with a point-shaped bulge or a strip-shaped bulge 4.2. The bottom of the small trough body 4.1 can be hollowed 4.3 by the downward part, and the reason for hollowing the downward part is as follows: the height of the upper surface of the liquid is constant in the case of two different liquid usage amounts.
Referring to fig. 12, the processing method of the device for incubation washing treatment of biological sample membrane or gel is characterized in that the device is assembled by a basket washer 3, a basket washer 1 and a basket washer 2, and then the assembled three are inserted into a washing tank 4 to cooperate with the washing tank, and a shifting tooth 3.3 or a gear 3.4 on the basket washer hooks a basket washer opening 2.1 through a basket washer opening 1.3 on the basket washer wall; under the action of external downward pressure or gears, the washing basket is driven to slide along the inner wall of the washing basket frame, the biological sample membrane or glue attached to the inner wall of the washing basket moves irregularly along with the movement of the washing basket in a circular operation direction shown by an arrow in the figure, and repeatedly passes through incubation washing liquid at the bottom of the washing tank 4 to finish the incubation washing process of the biological sample membrane.
Referring to fig. 13-15, the apparatus for incubation washing treatment of biological sample film or gel comprises a control computer, a vertical displacement motor 5 disposed on the top of the apparatus, and a horizontal displacement motor 6 disposed on the top of the apparatus; the vertical displacement motor 5 drives a matching body consisting of the basket washing poking device 3, the basket washing frame 1 and the basket washing 2 to move up and down in the washing tank; the horizontal displacement motor 6 drives the matching body to do horizontal motion on the washing tank 4, so as to finish the incubation washing work of the biological sample membrane or glue; the above control computer, vertical displacement motor 5, horizontal displacement motor 6 may also be replaced as a whole by a robot arm (e.g. SCARA robot arm, palletizer robot arm).
Referring to fig. 16 and 17, the machine for cooperating with the above mentioned device for incubation and washing treatment of biological sample membrane or gel comprises a central column structure 7 located in the middle of the washing tank, and a cross beam 8 erected on the central column structure through a "n" shaped connecting piece 9, wherein the cross beam 8 drives a plurality of basket washing pokers 3, basket washing frames 1, and baskets washing 2 to form a cooperating body, which moves up and down in the washing tank 4 and moves horizontally above the washing tank, thereby completing the incubation and washing work of blotting membrane; the upper cover of the center post structure 7 and the peripheral wall of the center post structure form a gap 7.1 which is opened downwards and allows the 'n' shaped connecting piece 9 to freely move back and forth and up and down through the gap.
Referring to fig. 18 and 19, a machine for cooperating with the above mentioned device for incubation and washing treatment of a biological sample membrane or gel comprises a self-moving machine block 10 for driving a washing assembly to move up and down, back and forth, and for washing the blotting membrane; the motor 10.1 at the lower part of the machine square is used for driving the crawler to implement the front and back operation of the machine square, and the motor 10.2 is used for driving the upper part of the machine square to implement the up and down motion; the complementary clamping groove 10.3 and the convex strip 10.4 are used for involution limiting of the upper part and the lower part of the square block of the machine; the cavity 11.5 is used for arranging a computer control part; the upper part of the machine square block is connected with a washing combination in a left-right mode, so that the washing combination can carry out washing operation of the blotting membrane and drive a matching body consisting of a basket washing poking device 3, a basket washing frame 1 and a basket washing 2 to move up and down in a washing tank 4 and horizontally move above the washing tank 4, and incubation washing operation of the biological sample membrane or glue is carried out.
Referring to fig. 20, the washing tub 4 can be coupled to a container 11 containing a plurality of pre-stored washing liquids; the pressure in the container changes, and the washing liquid enters and exits from the bottom of the washing tank through the pipeline and is used for incubation and washing.
Referring to fig. 21, as a preferred embodiment, an inner lining structure may be provided in the wash basket 2. The material of the washing basket can be the same as that of the washing basket, the shape of the washing basket can be cylindrical or irregular cylindrical close to that of the washing basket, but no opening is formed, and the perimeter of the washing basket is shorter than that of the washing basket; the lining structure acts as: 1, limiting the range of motion of the washed film. 2, the liquid is beneficial to be concentrated on two sides of the washed membrane through the surface tension.
The implementation details are as follows:
the washing combination can complete the washing function of the biological sample membrane through the cooperation of the washing basket poking device 3, the washing basket frame 1 and the washing basket 2 and the washing tank.
When the washing combination of the washing basket poking tooth poking cover is selected for incubation and washing, the biological sample membrane is attached to the inner wall of the washing basket. The poking teeth on the poking cover of the washing basket penetrate through the openings on the front wall and the rear wall of the washing basket frame to clamp the horizontal strip-shaped washing basket opening 2.1 of the washing basket. Under the action of the downward pressure of the external force, the washing basket poking cover moves downwards, and the return spring 3.2 is compressed. The shifting teeth 3.3 at one side which moves downwards drive the washing basket to do irregular circular motion under the limitation of the washing basket frame and the washing tank, and meanwhile, the washing basket slides over the shifting teeth at the other side. When the external force is relieved, the reset spring 3.2 releases the washing basket to move the toggle cover upwards. When the washing basket moves upwards, the shifting teeth 3.3 at the other side of the shifting cover of the washing basket drive the washing basket. Under the limitation of the basket washing frame and the washing tank, the basket washing frame makes a circular motion, and meanwhile, the basket washing slides over the shifting teeth 3.3 on the other side; the biological sample membrane attached to the inner wall of the washing basket repeatedly passes through the incubation washing liquid at the bottom of the washing tank along with the circular motion of the washing basket, so that the incubation washing process of the biological sample membrane is completed.
A washing combination of a gear shifting cover of the washing basket is selected, a gear driven by a motor penetrates through an opening 1.3 in the washing basket frame, and the washing basket is shifted to finish the washing work.
A top dual motor machine design for washing can be implemented using the above-described washing combination in combination with a multi-tank washing process. For example, under the control of a computer, a horizontal displacement motor 6 and a vertical displacement motor 5 which are arranged at the top of the machine drive a matching body consisting of a basket washing poking device 3, a basket washing frame 1 and a basket washing 2 to move up and down in a washing tank 4 and horizontally move above the washing tank, so that the work of incubating and washing the blotting membrane is completed.
Another design of a liner structure machine for biofilm washing can also be implemented using the above-described washing combination. For example, under the control of a computer, the motor in the center pillar structure 10.1 drives the matching body of the basket washing poking device 3, the basket washing frame 1 and the basket washing 2 through the 'n' -shaped connecting piece 10.2 to move up and down in the washing tank 4 and horizontally move above the washing tank, thereby completing the work of blotting membrane incubation and washing. The gap 7.1 which is formed by the upper cover of the center post structure and the peripheral wall of the center post structure and is opened downwards can allow the 'n' -shaped connecting piece 9 to freely move back and forth and up and down through the gap. The advantage of this design is that the liquid in the wash is not easily able to enter the machine and damage it.
Using the above-described washing combination, the incubation and washing can also be accomplished with multi-dimensional movement of the universal robotic arm 12.
Using the above-described washing combination, it can be used in another embodiment of the self-propelled machine block; a self-moving machine block 10 which drives the washing combination to move up and down in the washing tank 4 and can move back and forth to wash the blotting membrane; the motor 10.1 at the lower part of the machine square is used for driving the crawler to implement the front and back operation of the machine square, and the motor 10.2 is used for driving the upper part of the machine square to implement the up and down motion; the complementary clamping groove 10.3 and the convex strip 10.4 are used for involution limiting of the upper part and the lower part of the square block of the machine; the cavity 10.5 is used for arranging a computer control part; the upper part 3 of the machine square block is connected with a washing combination in a left-right mode, so that the washing combination can carry out washing operation of the blotting membrane and drive a matching body consisting of a basket washing poking device, a basket washing frame 1 and a basket washing 2 to move up and down in a washing tank 4 and horizontally move above the washing tank 4, and incubation washing operation of the biological sample membrane or glue is carried out.
Using the above washing combination, it can be used in another pre-existing liquid set embodiment. For example, a washing assembly that is moved by a gear movement may be coupled to a container 11 containing a plurality of pre-stored washing liquids. The pressure of the container changes, liquid enters and exits the bottom of the washing tank 4 through the pipeline 11.1, the gear drives the washing basket 4, and the sample moves to finish incubation and washing work.
Aiming at the market condition and the defects of the existing machine, the invention adopts a completely new design concept, and can perfectly realize the three requirements: 1, uniformly incubating and washing; 2, using as few antibodies as possible; 3, avoiding cross contamination at one time. And the instrument has simple structure and proper price, and can be accepted by a laboratory user. The machine of the invention can be further extended to process a variety of different biological sample films (gels) according to similar principles. Therefore, the product of the invention can perfectly replace various existing inapplicable expensive machines, is applied to the field of processing various biological sample membranes (gels), and is released from heavy manual labor in tens of millions of laboratories.
The foregoing shows and describes the general principles and features of the present invention, together with the advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only for the purpose of illustrating the structural relationship and principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A device for incubation washing treatment of biological sample membranes or gels is characterized by comprising a washing basket frame (1), a washing basket (2) capable of being arranged in the washing basket frame, a washing basket poking device (3) for poking the washing basket to rotate in the washing basket frame (1) and a washing tank (4) for placing the washing basket frame (1) and incubating washing liquid; the washing basket poking device (3), the washing basket frame (1) and the washing basket (2) are assembled and then inserted into the washing tank (4) to cooperate with the washing tank, and poking teeth (3.3) or gears (3.4) on the washing basket poking device hook the washing basket opening (2.1) through the washing basket frame opening (1.3) on the wall of the washing basket frame; under the action of external downward pressure or gears, the washing basket is driven to slide along the inner wall of the washing basket frame, the biological sample membrane or glue attached to the inner wall of the washing basket does irregular circular motion along with the motion of the washing basket, and repeatedly passes through incubation washing liquid at the bottom of the washing tank (4) to finish the incubation washing process of the biological sample membrane.
2. Device for the incubation washing treatment of biological sample membranes or gels according to claim 1, characterized in that; the front and rear walls of the basket washing frame (1) are rectangular thin walls, the upper part of the front and rear walls is connected with a semicircular arc-shaped thin upper wall (1.1) to form a smooth curved surface, the left and right ends of the front and rear walls are provided with elliptic frames (1.2), the lower end of the basket washing frame (1) is open, and the walls are provided with a plurality of basket washing frame openings (1.3); the inner side of the basket washing frame is provided with a plurality of vertical and parallel strip-shaped bulges (1.4) which are slightly higher than the wall surface.
3. The device for the incubation washing treatment of the biological sample membrane or gel according to claim 1, characterized in that the washing basket (2) is a cylinder rolled by a flexible sheet material, the surface of the cylinder is provided with a washing basket opening (2.1) for the poking of the poking teeth, and the inner surface is provided with a punctiform or other forms of micro-protrusions (2.2).
4. The device for the incubation washing treatment of biological sample membranes or gels according to claim 1, characterised in that said basket shifter (3) is a basket-shifting tooth-shifting cover or a basket-gear shifting cover; the washing basket shifting tooth shifting cover consists of a shell (3.1), a reset spring (3.2) and shifting teeth (3.3), after penetrating through a washing basket frame, the hook surface of the shifting teeth can hook and shift the washing basket, and the smooth surface can enable the washing basket to slide through the surface of the washing basket; the basket washing gear poking cover pokes the basket through the rotating gear (3.4) after penetrating through the basket washing frame.
5. The device for the incubation washing treatment of the biological sample membrane or glue according to claim 1, characterized in that the washing tank (4) is a cuboid structure with an open upper end, the inside of the washing tank (4) is divided into a plurality of small rectangular tanks (4.1), the bottoms of the small tanks (4.1) are semi-cylindrical, and the radian of the cambered surface is consistent with the radian of the lower part of the washing basket frame (1); the bottom of the small trough body (4.1) can be attached to the bottom of the washing trough (4) or suspended; the inner side surface of the bottom of the small groove body (4.1) is provided with a point-shaped or strip-shaped bulge (4.2), and the bottom of the small groove body (4.1) can be hollowed-out (4.3) by a downward part.
6. Machine cooperating with the device for the incubation washing treatment of biological sample membranes or gels according to claim 1, characterised by comprising a control computer, a vertical displacement motor (5) placed on top of the machine, a horizontal displacement motor (6) placed on top of the machine; the vertical displacement motor (5) drives the washing basket poking device (3), the washing basket frame (1) and the washing basket (2) to form a matching body which moves up and down in the washing tank; the horizontal displacement motor (6) drives the matching body to do horizontal motion on the washing tank, so as to finish the incubation and washing work of the biological sample membrane or glue; the control computer, the vertical displacement motor (5) and the horizontal displacement motor (6) can be replaced by the mechanical arm as a whole.
7. The machine matched with the device for incubation washing treatment of the biological sample membrane or glue according to claim 1 is characterized by comprising a central column structure (7) positioned in the middle of the washing tank and a cross beam (8) erected on the central column structure through a 'n' -shaped connecting piece (9), wherein the cross beam (8) drives a plurality of basket washing pokers (3), basket washing frames (1) and basket washing frames (2) to form a matched body which moves up and down in the washing tank and moves horizontally on the washing tank to finish the incubation washing work of the blotting membrane; the upper cover of the center post structure and the peripheral wall of the center post structure form a gap (7.1) which is opened downwards and can allow the 'n' shaped connecting piece 9 to freely move up and down through the gap.
8. The machine which is matched with the device for incubation washing treatment of the biological sample membrane or glue according to claim 1 is characterized by comprising a machine block (10) which can move by itself and drives a washing combination to move up and down, back and forth to implement washing operation of the blotting membrane; the motor (10.1) at the lower part of the machine square is used for driving the crawler to implement the front-back running of the machine square, and the motor (10.2) is used for driving the upper part of the machine square to implement the up-down running; the complementary clamping groove (10.3) and the convex strip (10.4) are used for involution limiting of the upper part and the lower part of the square block of the machine; the cavity (10.5) is used for arranging the computer control part; the upper part of the machine square block is connected with a washing combination in a left-right mode, so that the washing combination can carry out washing operation of the blotting membrane and drive a matching body consisting of a basket washing poking device (3), a basket washing frame (1) and a basket washing (2) to move up and down in the washing tank and horizontally move above the washing tank, and incubation washing operation of the biological sample membrane or glue is carried out.
9. Device for the incubation washing treatment of biological sample membranes or gels according to claim 1, characterised in that said washing tank (4) is cooperable with a container (11) containing a plurality of pre-stored washing liquids; the pressure in the container changes, and the washing liquid enters and exits from the bottom of the washing tank through the pipeline and is used for incubation and washing.
10. The device for the incubation washing treatment of biological sample membranes or gels according to any of claims 1 to 9, characterized in that a lining structure with the same width as the basket is arranged in the basket (2); the material is the same as that of the washing basket (2); the shape is cylindrical or irregular cylindrical close to the washing basket, but has no opening and the perimeter is smaller than that of the washing basket.
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| CN201811264588.6A CN109507431B (en) | 2018-10-29 | 2018-10-29 | Device, machine and method for incubation washing treatment of biological sample membrane or glue |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811264588.6A CN109507431B (en) | 2018-10-29 | 2018-10-29 | Device, machine and method for incubation washing treatment of biological sample membrane or glue |
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| Publication Number | Publication Date |
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| CN109507431A CN109507431A (en) | 2019-03-22 |
| CN109507431B true CN109507431B (en) | 2022-03-04 |
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| CN111190021B (en) * | 2020-02-22 | 2023-08-11 | 武汉泰沃科技有限责任公司 | Biological sample processing box |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103913365A (en) * | 2014-04-04 | 2014-07-09 | 田丰 | Automatic processor of biological sample film |
| CN107243480A (en) * | 2017-07-12 | 2017-10-13 | 童贤波 | A kind of medicine equipment high-efficiency washing device |
| CN107525841A (en) * | 2017-06-02 | 2017-12-29 | 吴荣荣 | A kind of novel protein trace instrument and its test method |
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2018
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103913365A (en) * | 2014-04-04 | 2014-07-09 | 田丰 | Automatic processor of biological sample film |
| CN107525841A (en) * | 2017-06-02 | 2017-12-29 | 吴荣荣 | A kind of novel protein trace instrument and its test method |
| CN107243480A (en) * | 2017-07-12 | 2017-10-13 | 童贤波 | A kind of medicine equipment high-efficiency washing device |
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Effective date of registration: 20211222 Address after: 430073 Room 518, building B1, R & D building, zone B, C and D, Wuhan National biological industry base project, 666 Gaoxin Avenue, Donghu New Technology Development Zone, Wuhan City, Hubei Province Applicant after: Wuhan Taiwo Technology Co.,Ltd. Address before: No.12, no.395-043, Luoshi Road, Hongshan District, Wuhan City, Hubei Province, 430000 Applicant before: Wang Guofu Applicant before: Liu suheng |
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