CN109496683B - Method for producing homogeneous dispersion type edible mushroom strain - Google Patents
Method for producing homogeneous dispersion type edible mushroom strain Download PDFInfo
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- CN109496683B CN109496683B CN201811456584.8A CN201811456584A CN109496683B CN 109496683 B CN109496683 B CN 109496683B CN 201811456584 A CN201811456584 A CN 201811456584A CN 109496683 B CN109496683 B CN 109496683B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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Abstract
The invention discloses a seed production method of a homogeneous dispersion type edible fungus strain, which comprises the following steps: (1) culturing liquid strains, a, preparing a liquid strain culture medium; b. sterilizing and inoculating, namely sterilizing the liquid culture medium by using a high-temperature sterilization method, and inoculating the original strain after cooling to room temperature; c. liquid strain deep culture; (2) preparing a granular strain, and a. preparing a granular strain culture medium; b. sterilizing the granular strain culture medium, and cooling to room temperature; c. inoculating the liquid strain obtained in the step (1) into a granular strain culture medium, and culturing into granular strains; (3) preparing powdery strains, a, drying the granular strains obtained in the step (2) at low temperature, and crushing the granular strains into powdery strains; b. packaging with air-permeable film bag, and storing at 0-4 deg.C; (4) and (5) activating the strains.
Description
Technical Field
The invention relates to a production method of edible fungi, in particular to a seed production method of a homogeneous dispersion type edible fungus strain.
Background
The edible fungi are popular with people due to the rich nutritive value, become common food for people and have large market demand. More and more edible fungus producers are available, and some places and even families are engaged in edible fungus production. However, the edible fungus species always troubles the edible fungus production in China.
At present, domestic edible fungus strains are divided into solid strains and liquid strains. In the prior art, the two strains have the problems of poor dispersion degree and poor uniformity, so that the strains are slow to germinate, irregular and irregular colonies, inconsistent hyphae growth and large in fungus age difference, the hyphae growth and development are poor, and the yield and the quality of the edible fungi are directly influenced.
On the other hand, the edible fungus seed production in China still stays in the traditional scattered workshop type production mode, scattered farmers use the edible fungus seeds by themselves, the production technology and equipment conditions are simple and crude, the seed quality is poor, and the phenomenon of production damage caused by the seed quality problem is serious. According to incomplete investigation and statistics, the production loss of edible fungi in China caused by seeds accounts for more than 30%. The production technology of edible fungi seeds is uneven, and the quality of the edible fungi is difficult to ensure. The edible fungus seeds in the prior art can not be uniformly stored, transported and arranged to scattered growers. Therefore, the development of domestic edible fungus production is restricted.
Finding an applicable edible fungus strain culture method for culturing a homogeneous dispersion type edible fungus strain is a technical problem which needs to be solved urgently in the technical field.
Disclosure of Invention
The invention aims to provide a suitable edible fungus strain production method for culturing a homogeneous dispersion type edible fungus strain.
In order to solve the technical problem, the seed production method of the homogeneous dispersion type edible fungus strain comprises the following steps:
(1) culturing liquid spawn
a. Preparing a liquid strain culture medium;
b. sterilizing and inoculating, namely sterilizing the liquid culture medium by using a high-temperature sterilization method, and inoculating the original strain after cooling to room temperature;
c. liquid strain submerged culture, culture conditions: the temperature is 24 +/-2 ℃, the vibration frequency is 156-;
(2) preparation of granular strains
a. Preparing a granular strain culture medium;
b. sterilizing the granular strain culture medium, and cooling to room temperature;
c. inoculating the liquid strain obtained in the step (1) into a granular strain culture medium, and culturing into granular strains;
(3) preparation of powdery Strain
a. Drying the granular strains obtained in the step (2) at low temperature, and crushing into powdery strains;
b. packaging with air-permeable film bag, and storing at 0-4 deg.C;
(4) strain activation
1-2 days before the powdery strain is used, adding the strain into 6-7 times of strain nutrient solution, and culturing at 24 + -2 deg.C for 24-36 hr to activate strain activity.
Preferably, the granular strains are prepared by fermenting with compound strains, adjusting pH to 5-7, C/N to 30-40:1, and water content to 50-60%, granulating to obtain complete edible fungus granular matrix, and culturing to obtain granular strains.
Preferably, the composite strain is a mixed bacterial liquid prepared by actinomycetes, photosynthetic bacteria, trichoderma and saccharomyces cerevisiae bacterial colonies according to the proportion of 1-2:3-4:3-4: 2-3.
Preferably, the liquid strain culture medium is prepared from the following raw materials in parts by weight: 4-6 parts of corn flour, 2-4 parts of soybean milk and 1 part of white sugar.
Preferably, the granular strain culture medium is prepared by mixing straws and couch grass in a weight ratio of 2-3:3-4, crushing into powder with the particle size of less than or equal to 40 meshes and drying in the sun.
Preferably, the liquid strain is cultured and sterilized at high temperature under the rated pressure of 1.5Pa and at the temperature of 108-126 ℃ for 1.5-2 hours.
Preferably, the strain nutrient solution in the strain activation step is prepared from the following raw materials in parts by weight: white sugar 1, soybean milk 3, corn flour 3, monopotassium phosphate 0.1 and dipotassium phosphate 0.1.
The method is adopted, the raw materials are fermented by the composite bacteria, the composite bacteria are combined into special high-efficiency flora, then the special high-quality high-efficiency matrix (particles) is prepared by blending, and the strains in three shapes from liquid state, particles to powder state are transformed and refined, so that the solid powdery homogeneous dispersion type edible fungus strain is obtained. The raw materials are from coarse to fine, the hyphae grow from the outside to the inside, and the dispersion degree and the uniformity of the strains are good. The method adds the bacteria-promoting nutrient into the powdery strain before use for activation, so that the strain is quickly compounded and has strong reproductive capacity.
The method of the invention completely overcomes the defects of the prior art, and the prepared homogeneous dispersion type strain has high purity and strong fertility, is convenient for packaging, transportation and storage and is easy to popularize.
Compared with the prior art, the invention has the following advantages:
(1) the technical process is advanced. The strain has high purity, strong fertility and convenient transportation and use, and solves the problems of weak hypha activity and high mixed bacteria rate of the traditional solid strain.
(2) Solves the problems of inconvenient preservation of liquid strains, high difficulty in controlling mixed strains and difficult popularization. Belongs to the new edible fungus seed production technology in China.
Through experimental detection, the comparison between the homogeneous dispersion type edible fungus strain produced by the method of the invention and similar technologies at home and abroad is shown in table 1:
table 1 the patent method is compared with similar techniques at home and abroad for analysis:
Detailed Description
The present invention is described in detail below:
the seed production method of the homogeneous dispersion type edible fungus strain comprises the following steps:
(1) culturing liquid spawn
a. Preparing a liquid strain culture medium. The liquid strain culture medium is prepared from the following raw materials in parts by weight: corn flour 6, soybean milk 4 and white sugar 1.
b. And (5) sterilizing and inoculating. Sterilizing the liquid culture medium by a high-temperature sterilization method, and inoculating the stock after cooling to room temperature. The liquid strain is cultured and sterilized at high temperature, the rated pressure is 1.5Pa, the temperature is 126 ℃, and the temperature is kept for 2 hours.
c. Liquid strain submerged culture, culture conditions: the temperature is 24 ℃, the vibration frequency is 178r/min, the culture time is 7 days, and the pH value is 6.5.
(2) Preparation of granular strains
a. Preparing a granular strain culture medium. The granular strain culture medium is prepared by mixing straws and couch grass according to the weight ratio of 3:4, crushing into 40-mesh powder and drying in the sun.
b. Sterilizing the granular strain culture medium, and cooling to room temperature.
c. Inoculating the liquid strain obtained in the step (1) into a granular strain culture medium, and culturing to obtain granular strains. The granular strain is prepared by fermenting with composite strain, adjusting pH to 7, C/N to 40:1, and water content to 60%, granulating to obtain complete edible fungus granular matrix, and culturing to obtain granular strain. The composite strain is a mixed bacterial liquid prepared by actinomycetes, photosynthetic bacteria, trichoderma and beer yeast colonies according to the colony number ratio of 2:4:4: 3.
(3) Preparation of powdery Strain
a. Drying the granular strains obtained in the step (2) at low temperature, and crushing into powdery strains.
b. Packaging with air-permeable film bag, and storing at 0 deg.C.
(4) Strain activation
2 days before the powdery strain is used, the strain is added into a strain nutrient solution with the weight being 7 times of that of the strain and cultured for 36 hours at 24 ℃ so as to activate the strain activity. The strain nutrient solution is prepared from the following raw materials in parts by weight: white sugar 1, soybean milk 3, corn flour 3, monopotassium phosphate 0.1 and dipotassium phosphate 0.1.
The above is just one embodiment. The object of the process is achieved by the conditions within the scope of the process claims of the invention.
Claims (1)
1. A seed production method of a homogeneous dispersion type edible fungus strain is characterized by comprising the following steps:
(1) culturing liquid spawn
a. Preparing a liquid strain culture medium; the liquid strain culture medium is prepared from the following raw materials in parts by weight: 4-6 parts of corn flour, 2-4 parts of soybean milk and 1 part of white sugar;
b. sterilizing and inoculating, namely sterilizing the liquid culture medium by using a high-temperature sterilization method, and inoculating the original strain after cooling to room temperature;
c. liquid strain submerged culture, culture conditions: the temperature is 24 +/-2 ℃, the vibration frequency is 156-;
the liquid strain is cultured and sterilized at high temperature, the rated pressure is 1.5Pa, the temperature is 108-;
(2) preparation of granular strains
a. Preparing a granular strain culture medium; the granular strain culture medium is prepared by mixing straws and couch grass according to the weight ratio of 2-3:3-4, crushing into powder with the particle size of less than or equal to 40 meshes and drying in the sun;
b. sterilizing the granular strain culture medium, and cooling to room temperature;
c. inoculating the liquid strain obtained in the step (1) into a granular strain culture medium, and culturing into granular strains;
the granular strains are prepared by fermenting with compound strains, adjusting pH to 5-7, C/N to 30-40:1, and water content to 50-60%, granulating to obtain complete edible fungus granular matrix, and culturing to obtain granular strains;
the composite strain is a mixed bacterial liquid prepared by actinomycetes, photosynthetic bacteria, trichoderma and saccharomyces cerevisiae bacterial colonies according to the proportion of 1-2:3-4:3-4: 2-3;
(3) preparation of powdery Strain
a. Drying the granular strains obtained in the step (2) at low temperature, and crushing into powdery strains;
b. packaging with air-permeable film bag, and storing at 0-4 deg.C;
(4) strain activation
1-2 days before the powdery strain is used, adding the strain into a strain nutrient solution with the weight of 6-7 times of the strain nutrient solution, and culturing at 24 +/-2 ℃ for 24-36 hours to activate the strain activity;
the strain nutrient solution in the strain activation step is prepared from the following raw materials in parts by weight: white sugar 1, soybean milk 3, corn flour 3, monopotassium phosphate 0.1 and dipotassium phosphate 0.1.
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| CN1058288C (en) * | 1994-10-15 | 2000-11-08 | 华启洪 | Production method of cryptoporus volvatus powder |
| CN100415249C (en) * | 2005-04-01 | 2008-09-03 | 任岛峰 | Production method of edible pharmaceutical spherical particle bacterial |
| CN100532541C (en) * | 2006-11-17 | 2009-08-26 | 湖南豫园生物科技有限公司 | Method for preparing bacterial powder |
| CN101066029A (en) * | 2007-06-05 | 2007-11-07 | 浙江大学 | Process of preparing colloidal edible fungus seed |
| CN102120972B (en) * | 2010-12-08 | 2013-07-24 | 浙江京新药业股份有限公司 | Preparation method of Bacillus licheniformis powder and microbial ecological agent |
| CN202085516U (en) * | 2011-05-17 | 2011-12-28 | 华中农业大学 | Automatic spawn inoculation machine for bag-cultivation edible fungi branch |
| KR20130085782A (en) * | 2012-01-20 | 2013-07-30 | 고재칠 | Hair product composition for promoting growth of hair and method of manufacturing for the same |
| CN104529614A (en) * | 2014-12-03 | 2015-04-22 | 杨德山 | Preparation methods and products of powdery comprehensive enzyme strain and comprehensive enzyme bacterial fertilizer |
| CN104718996B (en) * | 2015-04-13 | 2017-11-24 | 青岛联合菌业科技发展有限公司 | The preparation method of edible mushroom solid liquefaction strain |
| CN106916759A (en) * | 2015-12-24 | 2017-07-04 | 新疆康美瑞农业发展有限公司 | A kind of preparation method of high anti-oxidation lactic acid bacteria powder |
| CN107142221A (en) * | 2017-06-26 | 2017-09-08 | 安庆师范大学 | A kind of preparation method and application of edible and medical fungi composite water conservation granular bacteria strain |
| CN108030097B (en) * | 2017-12-28 | 2021-03-30 | 福建拓天生物科技有限公司 | Probiotic freeze-dried powder for stimulating appetite of children |
| CN108514073B (en) * | 2018-04-13 | 2021-06-08 | 湖南省食用菌研究所 | Method for preparing natural fresh-keeping nutritional wet rice noodles by adding fungus culture solution |
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