CN109439711A - A kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique - Google Patents
A kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique Download PDFInfo
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- CN109439711A CN109439711A CN201811344925.2A CN201811344925A CN109439711A CN 109439711 A CN109439711 A CN 109439711A CN 201811344925 A CN201811344925 A CN 201811344925A CN 109439711 A CN109439711 A CN 109439711A
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- polyurethane foam
- geniposide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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Abstract
In the present invention, using the polyurethane foam of unit aperture as the carrier of immobilized cellulase, obtain a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique, so that cellulase is fixed in the hole unit of polyurethane foam in the form embedded, the contact area of cellulose enzyme-to-substrate is controlled by foamed material and the optimal control percent opening and aperture size of dosage, in the case where guaranteeing certain geniposide conversion rate, increase has added cellulase to reuse number and service efficiency.The experimental results showed that, after 7 times are recycled, polyurethane foam immobilized cellulase geniposide accumulation conversion ratio be etc. quality free cellulose enzyme 413.3%, and the applicable enzyme of the technique and the optional range of glucosides are very big, can be according to the difference of the glucosides of required conversion, the different invertase of selection immobilization.In addition, instrument and equipment used in this technique is simple and easy to operate, preparation cost is low, is easily enlarged industrialization.
Description
Technical field
The present invention relates to a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion techniques, in particular to
It is a kind of with novel the polyurethane foam of unit aperture as carrier, with investment immobilized cellulase and is converted glucosides
New process.
Background technique
Geniposide (geniposide) is one of main component of cape jasmine, belongs to iridoid glycosides compound.It is modern
Pharmacological research shows that geniposide has anti-inflammatory, resisting rheumatoid arthritis, anti-alzheimer's disease, anti-diabetic and nerve
The multiple pharmacological effects such as protection.The hydrolysate Geniposide of geniposide has the pharmacological activity such as liver protection, decompression, antitumor, and
And it is often used as genipin.
Cellulase (β-Isosorbide-5-Nitrae-glucan -4- glucan hydrolase) is a kind of complex enzyme, mainly by circumscribed beta glucan
The composition such as enzyme, Endo-β-glucanase and beta-glucosidase and zytase.Its is from a wealth of sources, active higher, enzymatic reaction
Mild condition is in recent years by one of enzyme of primary study.Application of cellulase range is wider, but service efficiency is lower, and
It by cellulase immobilization, can be used for multiple times, greatly improve service efficiency, therefore be to the research of cellulase immobilization
Enable the important channel that cellulase more makes full use of.
Polyurethane foam is one of principal item of polyurethane material.In soft bubble and part semi-rigid foam material therein
Portion has connection and staggered hole cellular construction, and not only specific surface area is high, and the hole unit in suitable aperture can accommodate moisture
The features such as son passes through, its matter is soft in addition, resilience is high make to be carried on cellulase therein can not only be adequately exposed to it is water-soluble
Property substrate, additionally it is possible to make enzymatic reaction generate product be discharged in time, it is anti-to enzymatic to reduce production concentration to a certain extent
The influence answered.
Cellulase is fixed in its hole unit by polyurethane foam, makes it while guaranteeing activity itself, again
It can continuous several times use.Immobilized cellulase has been widely used in various fields, especially based on medicine, biology etc..
This method is simple mild, repeatability is strong, and can cellulose resource make full use of.Currently, the method for immobilized cellulase
So that cellulase is crosslinking in plural gel or porous microsphere of transformation etc., and directly embeds fiber independent of polyurethane foam
Plain enzyme to immobilized cellulase report.This method, which prepares resulting immobilized cellulase, can be good at guaranteeing fiber
The plain original enzyme activity of enzyme, can also simultaneously repeatedly use, and fixation support raw material are simple and easy to get, are easy to industry
Metaplasia produces.
Summary of the invention
In order to improve the defects of cellulase utilization rate is low, waste is serious, it is solid that the present invention provides a kind of polyurethane foams
Determine cellulose enzyme enzymatic geniposide conversion technique, in the case where guaranteeing enzymatic activity, increases the repetition for having added immobilised enzymes
Access times make cellulase be greatly improved the transformation efficiency of geniposide.
A kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique provided by the invention is by cellulose
Enzyme is fixed in polyurethane foam in the form embedded, and wherein the polyurethane foam carrier of this technique preparation and polyurethane foam are solid
The pattern for determining cellulose enzyme is as shown in Fig. 1.
A kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique provided by the invention, by poly-
The research of urethane foam carrier hole unit percent opening and aperture size, enables the cellulase being fixed in carrier in maximum journey
Substrate geniposide is contacted on degree, wherein the polyurethane foam carrier and polyurethane foam immobilized cellulase of this technique preparation
Pore-size distribution it is as shown in Fig. 2.
A kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique provided by the invention, is determining enzyme
The reuse number of cellulase has been increased considerably in active situation, wherein using the conversion ratio of geniposide as standard,
The reuse situation of the immobilised enzymes is determined, as shown in Fig. 3.
A kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique of the present invention, feature exist
In: any method is extracted into resulting cellulase powder, be added the deionized water of suitable proportion, polyalcohol, surfactant,
It is uniformly mixed in the polyurethane foam material of dibutyl tin dilaurate, a certain proportion of methyl diphenylene diisocyanate is added
Afterwards, 20s is stirred in 20 DEG C of water-baths, under the mechanical agitation that revolving speed is 500r/min, then solidified at room temperature for 24 hours, i.e.,
Polyurethane foam immobilized cellulase can be obtained.The immobilized cellulase that will be prepared, in suitable temperature, the condition of pH
Under, it is placed directly in geniposide aqueous solution, carries out enzymatic reaction 70min, take out immobilized cellulase, high temperature method makes molten
After enzyme inactivation in liquid, filter membrane and the genipin solution concentration generated with high effective liquid chromatography for measuring calculate geniposide
Conversion ratio.
Advantages of the present invention:
1. the present invention selects polyurethane foam as carrier, there is porous structure, specific surface area is larger；Mechanicalness, stabilization
Property is good；The advantages that nontoxic, the preparation particularly suitable for immobilised enzymes.
2. the present invention has many advantages, such as that instrument and equipment is simple, preparation is easy, low in cost, is easily enlarged industrialization.
3. gained immobilized cellulase of the invention can also be multiple outside the activity for guaranteeing original homogenous quantities cellulase
It reuses, effectively increases cellulase to the service efficiency of biological glucosides.
4. gained immobilized cellulase of the invention can not only make to be carried on cellulase therein and be adequately exposed to water
The substrate of dissolubility, additionally it is possible to which the product for generating enzymatic reaction is discharged in time, reduces production concentration to a certain extent to enzymatic
The influence of reaction provides new thinking for the research work of the carrier for immobilised enzymes.
Detailed description of the invention
Fig. 1 is polyurethane foam carrier (a) and polyurethane foam immobilized cellulase (b) pattern comparison diagram；
Fig. 2 is the graph of pore diameter distribution of polyurethane foam carrier (a) He polyurethane foam immobilized cellulase；
Fig. 3 is the reuse situation curve that polyurethane foam immobilized cellulase carries out enzymatic reaction to geniposide
Figure.
Specific embodiment
The embodiment of the present invention is described in further detail below: suitable powdery cellulase is put into suitable ratio
Example deionized water, polyalcohol, surfactant, dibutyl tin dilaurate polyurethane foam material in be uniformly mixed, add
After entering a certain proportion of methyl diphenylene diisocyanate, in 20 DEG C of water-baths, the mechanical agitation that revolving speed is 500r/min
Then lower stirring 20s solidifies for 24 hours at room temperature to get using polyurethane foam as the immobilized cellulase of carrier.It will prepare
Immobilized cellulase be placed directly in geniposide aqueous solution under conditions of suitable temperature, pH, carry out enzymatic
70min is reacted, takes out immobilized cellulase, after high temperature method inactivates the enzyme in solution, filter membrane simultaneously uses high performance liquid chromatography
The genipin solution concentration that method measurement generates, calculates geniposide conversion rate.In order to make the purpose of the present invention, technical solution and excellent
Point is more clearly understood, and with reference to embodiments, the present invention will be described in further detail.Specific implementation described herein
Example is not intended to limit the present invention only to explain the present invention.
Example 1:
The powdery cellulase that mass fraction is 30.0% is put into 1.3% deionized water, 37.5% poly- second two
The peaceful DC-193 of Kang Dao of alcohol 400,0.5%, 0.6% dibutyl tin dilaurate polyurethane foam material be uniformly mixed, add
After entering 30% methyl diphenylene diisocyanate, stirred in 20 DEG C of water-baths, under the mechanical agitation that revolving speed is 500r/min
Then 20s solidifies for 24 hours at room temperature to get using polyurethane foam as the immobilized cellulase of carrier.The fixation that will be prepared
Cellulose enzyme is placed directly in geniposide aqueous solution under conditions of 35 DEG C, pH=6, carries out enzymatic reaction 70min,
Immobilized cellulase is taken out, after high temperature method inactivates the enzyme in solution, filter membrane is simultaneously generated with high effective liquid chromatography for measuring
Genipin solution concentration, calculate geniposide conversion rate.As a result it can obtain, after 7 times are recycled, immobilized cellulase
Geniposide accumulate conversion ratio be etc. quality free cellulose enzyme 235.0%.
Example 2:
By mass fraction be 27.6% cellulase be put into 2.1% deionized water, 35.0% Macrogol 600,
0.7% Tween 80,0.4% dibutyl tin dilaurate polyurethane foam material in be uniformly mixed, be added 35.0% 2
After methylenebis phenyl isocyanate, 20s is stirred in 20 DEG C of water-baths, under the mechanical agitation that revolving speed is 500r/min, then
Solidify at room temperature for 24 hours to get using polyurethane foam as the immobilized cellulase of carrier.By the immobilization fibre prepared element
Enzyme is placed directly in geniposide aqueous solution under conditions of 15 DEG C, pH=3, carries out enzymatic reaction 70min, is taken out and is fixed
Cellulose enzyme, after high temperature method inactivates the enzyme in solution, filter membrane and the Geniposide generated with high effective liquid chromatography for measuring
Solution concentration calculates geniposide conversion rate.As a result it can obtain, after 7 times are recycled, the geniposide of immobilized cellulase
Accumulate conversion ratio be etc. quality free cellulose enzyme 215.8%.
Claims (10)
1. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique, comprising the following steps: by any side
Method extracts resulting cellulase powder, and deionized water, the polyalcohol, surfactant, tin dilaurate two of suitable proportion is added
It is uniformly mixed in the polyurethane foam material of butyl tin, after a certain proportion of methyl diphenylene diisocyanate is added, at 20 DEG C
20s is stirred in water-bath, under the mechanical agitation that revolving speed is 500r/min, then solidifies at room temperature for 24 hours, poly- ammonia can be obtained
Ester foam immobilized cellulase.The immobilized cellulase that will be prepared directly is put under conditions of suitable temperature, pH
It is placed in geniposide aqueous solution, carries out enzymatic reaction 70min, take out immobilized cellulase, high temperature method makes the enzyme in solution
After inactivation, filter membrane and the genipin solution concentration generated with high effective liquid chromatography for measuring calculate geniposide conversion rate.
2. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, cellulase is fixed in the hole unit of polyurethane foam in a manner of embedding.
3. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, the polyurethane foam for load is soft bubble or part semi-rigid bubble, and hole unit is aperture or half aperture knot
Structure.
4. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, cellulase powder can be resulting by any method, and additive amount range is reactant gross mass
10%~40%.
5. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, it is the equal than mixing of polyethylene glycol 400 or Macrogol 600 or both that Polyurethane carrier, which generates the polyalcohol in reaction,
Close object), amount ranges are the 25%~55% of reactant gross mass.
6. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, it is reactant gross mass that Polyurethane carrier, which generates the methyl diphenylene diisocyanate amount ranges in reaction,
23%~45%.
7. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, the deionized water amount ranges in reactant are the 0.5%~2.5% of reactant gross mass.
8. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, the surfactant that Polyurethane carrier generates in reaction can change polyurethane hole unit size and percent opening
Compound, is one of the peaceful DC-193 of Kang Dao, Tween 80, and amount ranges are the 0.5%~2.5% of reactant gross mass.
9. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, it is the 0.3% of reactant gross mass that Polyurethane carrier, which generates the dibutyl tin dilaurate amount ranges in reaction,
~1.0%.
10. a kind of polyurethane foam immobilized cellulase enzymatic geniposide conversion technique described in accordance with the claim 1,
It is characterized in that, in enzymatic reaction, the temperature range that polyurethane foam immobilized cellulase uses is 10~70 DEG C, and pH range is
2.0-7.0。
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090238811A1 (en) * | 2002-09-09 | 2009-09-24 | Mcdaniel C Steven | Enzymatic Antimicrobial and Antifouling Coatings and Polymeric Materials |
| CN101831469A (en) * | 2010-05-31 | 2010-09-15 | 福建农林大学 | Method for preparing gardenia red pigment by using immobilized enzyme |
| CN103695409A (en) * | 2013-12-27 | 2014-04-02 | 中国科学院合肥物质科学研究院 | Preparation method of immobilized enzyme and application of immobilized enzyme in geniposide conversion |
| CN103833949A (en) * | 2012-11-26 | 2014-06-04 | 中国农业大学 | Preparation method of polyurethane foam |
| CN104498469A (en) * | 2014-12-09 | 2015-04-08 | 陕西科技大学 | Preparation method of polyurethane foam immobilized microbe for treatment of azo dye sewage |
| WO2017156744A1 (en) * | 2016-03-17 | 2017-09-21 | Dsm Ip Assets B.V. | New gardenia blue pigment, preparation and use thereof |
| CN108017793A (en) * | 2017-11-27 | 2018-05-11 | 兰州大学白银产业技术研究院 | A kind of application being sustained in the preparation method and its chemical wastewater treatment of polyurethane mesh carrier |
-
2018
- 2018-11-13 CN CN201811344925.2A patent/CN109439711A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090238811A1 (en) * | 2002-09-09 | 2009-09-24 | Mcdaniel C Steven | Enzymatic Antimicrobial and Antifouling Coatings and Polymeric Materials |
| CN101831469A (en) * | 2010-05-31 | 2010-09-15 | 福建农林大学 | Method for preparing gardenia red pigment by using immobilized enzyme |
| CN103833949A (en) * | 2012-11-26 | 2014-06-04 | 中国农业大学 | Preparation method of polyurethane foam |
| CN103695409A (en) * | 2013-12-27 | 2014-04-02 | 中国科学院合肥物质科学研究院 | Preparation method of immobilized enzyme and application of immobilized enzyme in geniposide conversion |
| CN104498469A (en) * | 2014-12-09 | 2015-04-08 | 陕西科技大学 | Preparation method of polyurethane foam immobilized microbe for treatment of azo dye sewage |
| WO2017156744A1 (en) * | 2016-03-17 | 2017-09-21 | Dsm Ip Assets B.V. | New gardenia blue pigment, preparation and use thereof |
| CN108017793A (en) * | 2017-11-27 | 2018-05-11 | 兰州大学白银产业技术研究院 | A kind of application being sustained in the preparation method and its chemical wastewater treatment of polyurethane mesh carrier |
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Application publication date: 20190308 |