CN109439536A - Drink water bacteria fast culture device and total plate count rapid detection system - Google Patents
Drink water bacteria fast culture device and total plate count rapid detection system Download PDFInfo
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- CN109439536A CN109439536A CN201710710106.4A CN201710710106A CN109439536A CN 109439536 A CN109439536 A CN 109439536A CN 201710710106 A CN201710710106 A CN 201710710106A CN 109439536 A CN109439536 A CN 109439536A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 79
- 241000894006 Bacteria Species 0.000 title claims abstract description 35
- 238000001514 detection method Methods 0.000 title claims abstract description 25
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000012141 concentrate Substances 0.000 claims abstract description 5
- 239000003651 drinking water Substances 0.000 claims description 10
- 235000020188 drinking water Nutrition 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims 1
- 238000010835 comparative analysis Methods 0.000 abstract description 3
- 230000007812 deficiency Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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Abstract
One kind of the present invention drinks in water bacteria fast culture device and is provided with inlet and outlet on constant temperature water tank, inlet and outlet are connected separately with inlet and outlet pipes, temperature measuring device and LED light source are provided with above constant temperature water tank, constant temperature water tank inputs the unboiled water of bacterium to be cultivated by water inlet line, guarantee that the water temperature in constant temperature water tank is controlled in the optimum growth temperature of bacterium by temperature measuring device and temperature control equipment, the reproduction speed that can accelerate bacterium additionally by LED light irradiation, achievees the purpose that fast culture；The water of rapid detection system outlet conduit outflow of the present invention obtains concentrate by the filter membrane, which detects total plate count therein by resistance method, can achieve the purpose that comparative analysis with quicklook.
Description
Technical field
The present invention relates to purifier fields, drink water bacteria fast culture device and total plate count more particularly to one kind
Rapid detection system.
Background technique
With the raising of industrialization degree, the pollution of natural environment is also increasingly to deepen, and drinking water must as what is lived
It needs, people increasingly pay attention to the complete health of drinking water, therefore purifier comes into being.
But purifier quality currently on the market is irregular, bacterium colony is total in the drinking water filtered by purifier
The range whether number meets national regulation is unknown, influences the safe and healthy problem that people drink water in daily life.Cause
This, needs one kind that can make rapid detection system to the total plate count in application water, but in sales process, consumer needs
The comparison situation of total plate count can be intuitively seen, so being also required to provide a kind of application water bacteria fast culture device to train
Bacterium is supported as reference.
Summary of the invention
One of main object of the present invention is to overcome the disadvantage mentioned above of the prior art and deficiency, and it is thin to provide a kind of drinking water
Bacterium fast culture device.
It includes a constant temperature water tank that one kind, which drinking water bacteria fast culture device, and water inlet is provided on constant temperature water tank and is gone out
The mouth of a river, inlet and outlet are connected separately with inlet and outlet pipes, and temperature measurement dress is provided with above constant temperature water tank
It sets and LED light source, the temperature measuring device one end is set in constant temperature water tank, the LED light source is set to temperature measuring device one
Side.
The LED light source is red-light LED in one of the embodiments,.
It is provided with pH value adjusting apparatus above the constant temperature water tank in one of the embodiments, the pH value tester one end
It is set in the constant temperature water tank.
The constant temperature water tank is drum-shaped in one of the embodiments, which is provided with bracket, the branch
Frame upper end is installed with the temperature measuring device, LED light source and pH value tester.
The constant temperature water tank is transparent vessel in one of the embodiments,.
It is provided with temperature control equipment in the constant temperature water tank in one of the embodiments, the temperature control equipment packet
A single chip control unit, a heating device and a refrigerating plant, the heating device is included to pass through with refrigerating plant
It is electrically connected to single chip control unit, the temperature measuring device is electrically connected with single chip control unit, is measured and is filled by temperature
It sets and the temperature in constant temperature water tank is sent to single chip control unit, single chip control unit is surveyed according to preset temperature and temperature
The detection temperature of amount device transmission compares, and the operation of the heating device and refrigerating plant is controlled according to comparing result.
The two of the main object of the present invention are to overcome the disadvantage mentioned above of the prior art and deficiency, provide a kind of total plate count
Rapid detection system.
A kind of total plate count rapid detection system sheet includes to drink water bacteria fast culture device, drinks water bacteria and quickly trains
Supporting device includes outlet conduit, and the outlet conduit end is provided with the detection device of total plate count.
The detection device includes the filter membrane in ultra-fine hole in one of the embodiments, the outlet conduit outflow
Water obtains concentrate by the filter membrane, which detects total plate count therein by resistance method.
The aperture of the filter membrane is R, 0.6nm < R < 0.1um in one of the embodiments,.
The detection device is ATP fluorescence detector in one of the embodiments,.
One kind of the present invention drinks in water bacteria fast culture device and is provided with inlet and outlet on constant temperature water tank, water inlet
Mouth and water outlet are connected separately with inlet and outlet pipes, are provided with temperature measuring device and LED light above constant temperature water tank
Source, constant temperature water tank input the unboiled water of bacterium to be cultivated by water inlet line, are protected by temperature measuring device and temperature control equipment
The water temperature control in constant temperature water tank is demonstrate,proved in the optimum growth temperature of bacterium, bacterium can be accelerated additionally by LED light irradiation
Reproduction speed achievees the purpose that fast culture；The water of rapid detection system outlet conduit outflow of the present invention is obtained by the filter membrane
Concentrate is obtained, which detects total plate count therein by resistance method, can be with the mesh for reaching comparative analysis of quicklook
's.
Detailed description of the invention
Fig. 1 is a kind of structural representation for drinking water bacteria fast culture device and total plate count rapid detection system of the present invention
Figure；
Fig. 2 is a kind of part-structure for drinking water bacteria fast culture device and total plate count rapid detection system of Fig. 1 present invention
Schematic diagram.
Specific embodiment
To facilitate the understanding of the present invention, a more comprehensive description of the invention is given in the following sections with reference to the relevant attached drawings.It is provided in attached drawing
Better embodiment of the invention.But the invention can be realized in many different forms, however it is not limited to be retouched herein
The embodiment stated.On the contrary, the purpose of providing these embodiments is that making to understand the disclosure more saturating
It is thorough comprehensive.
It should be noted that it can be directly to separately when an element is considered as " connection " another element
One element may be simultaneously present centering elements.Term as used herein "vertical", "horizontal", "left" and "right"
And similar statement is for illustrative purposes only, is not meant to be the only embodiment.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more
Any and all combinations of relevant listed item.
Fig. 1 and Fig. 2 are please referred to, it includes a constant temperature water tank 1, the constant temperature water tank that one kind, which drinking water bacteria fast culture device,
Inlet and outlet are provided on 1, inlet and outlet are connected separately with water inlet line 2 and outlet conduit 3, the constant temperature
Temperature measuring device 4 and LED light source 5 are provided with above water tank 1, which measures 4 device one end and be set to the constant temperature water tank 1
Interior, the LED light source 5 is set to 4 side of temperature measuring device.
Wherein, the LED light source 5 is red-light LED, or nearly red-light LED, LED light source 5 is preferably COB light source, red
Light LED has the function of promotion bacterial reproduction, and wave band is preferably 630nm-640nm.
It is provided with pH value adjusting apparatus 6 above the constant temperature water tank 1, which is set to the thermostatted water
In case 1, pH value tester 6 is set to 5 side of LED light source, and the LED light source 5 is located at temperature measuring device 4 and pH value tester
Between 6.
The constant temperature water tank 1 is drum-shaped, which is provided with bracket 7, which extends upwardly to perseverance
1 bottom of constant temperature water tank is fixed in 1 top of reservoir, 7 lower end of bracket, which is installed with the temperature measuring device
4, LED light source 5 and pH value tester 6,.
The constant temperature water tank 1 is transparent vessel, can observe by the naked eye the situation in constant temperature water tank.
Temperature control equipment 8 is provided in the constant temperature water tank, which includes that a single-chip microcontroller control is single
First 81, heating devices 82 and a refrigerating plant 83, the heating device 82 is with refrigerating plant 83 by being electrically connected to
Single chip control unit 81, the temperature measuring device 4 are electrically connected with single chip control unit 81, pass through temperature measuring device 4
Temperature in constant temperature water tank is sent to single chip control unit 81, single chip control unit 81 is according to preset temperature and temperature
The detection temperature that measuring device 4 transmits compares, and the heating device 82 and refrigerating plant 83 are controlled according to comparing result
Operation.
Wherein, the water temperature in constant temperature water tank 1 should be controlled at 23 DEG C -28 DEG C, and preferably 24 DEG C.
One kind of the present invention drinks water bacteria fast culture device and guarantees perseverance by temperature measuring device and temperature control equipment
Water temperature control in reservoir can accelerate the breeding of bacterium additionally by LED light irradiation in the optimum growth temperature of bacterium
Speed achievees the purpose that fast culture.
A kind of total plate count rapid detection system comprising drinking water bacteria fast culture device, 3 end of outlet conduit
End is provided with the detection device 9 of total plate count.
Wherein, the detection device 9 includes the filter membrane in ultra-fine hole, and the water that the outlet conduit 3 flows out passes through the filtering
Film obtains concentrate, which detects total plate count therein by resistance method, can reach comparative analysis with quicklook
Purpose.
The aperture of the filter membrane is R, 0.6nm < R < 0.1um.
The detection device 9 is not limited to resistance method to detect total plate count, and the detection device 9 is ATP fluorescence detector.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not present
Contradiction all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. one kind drinks water bacteria fast culture device, it is characterised in that: including a constant temperature water tank, be provided on constant temperature water tank
Inlet and outlet, inlet and outlet are connected separately with inlet and outlet pipes, are provided with above constant temperature water tank
Temperature measuring device and LED light source, the temperature measuring device one end are set in constant temperature water tank, and the LED light source is set to temperature
Measuring device side.
2. one kind according to claim 1 drinks water bacteria fast culture device, it is characterised in that: the LED light source is
Red-light LED.
3. one kind according to claim 1 drinks water bacteria fast culture device, it is characterised in that: on the constant temperature water tank
Side is provided with pH value adjusting apparatus, which is set in the constant temperature water tank.
4. one kind according to claim 1 drinks water bacteria fast culture device, it is characterised in that: the constant temperature water tank is
Drum-shaped, the constant temperature water tank bottom are provided with bracket, which is installed with the temperature measuring device, LED light source and pH
Value tester.
5. one kind according to claim 1 drinks water bacteria fast culture device, it is characterised in that: the constant temperature water tank is
Transparent vessel.
6. one kind according to claim 1 drinks water bacteria fast culture device, it is characterised in that: in the constant temperature water tank
It is provided with temperature control equipment, which includes a single chip control unit, a heating device and a system
Device for cooling, the heating device and refrigerating plant by being electrically connected to single chip control unit, the temperature measuring device with
Single chip control unit electrical connection, is sent to single chip control unit for the temperature in constant temperature water tank by temperature measuring device,
The detection temperature that single chip control unit is transmitted according to preset temperature with temperature measuring device compares, according to comparing result
To control the operation of the heating device and refrigerating plant.
7. a kind of include a kind of quick detection system of total plate count for drinking water bacteria fast culture device described in claim 1
System, it is characterised in that: the outlet conduit end is provided with the detection device of total plate count.
8. one kind according to claim 7 drinks water bacteria fast culture device, it is characterised in that: the detection device packet
The filter membrane in ultra-fine hole is included, the water of the outlet conduit outflow obtains concentrate by the filter membrane, which passes through resistance
Method detects total plate count therein.
9. one kind according to claim 8 drinks water bacteria fast culture device, it is characterised in that: the hole of the filter membrane
Diameter is R, 0.6nm < R < 0.1um.
10. one kind according to claim 7 drinks water bacteria fast culture device, it is characterised in that: the detection device
For ATP fluorescence detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710710106.4A CN109439536A (en) | 2017-08-18 | 2017-08-18 | Drink water bacteria fast culture device and total plate count rapid detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710710106.4A CN109439536A (en) | 2017-08-18 | 2017-08-18 | Drink water bacteria fast culture device and total plate count rapid detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109439536A true CN109439536A (en) | 2019-03-08 |
Family
ID=65529958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710710106.4A Pending CN109439536A (en) | 2017-08-18 | 2017-08-18 | Drink water bacteria fast culture device and total plate count rapid detection system |
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| Country | Link |
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| CN (1) | CN109439536A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112011590A (en) * | 2020-08-27 | 2020-12-01 | 苏州联睿微半导体科技有限公司 | Method for detecting bacteria in drinking water |
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| CN112011590A (en) * | 2020-08-27 | 2020-12-01 | 苏州联睿微半导体科技有限公司 | Method for detecting bacteria in drinking water |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zeng Lihua Inventor after: Huang Guoren Inventor after: Chen Taiyuan Inventor before: Huang Guoren Inventor before: Chen Taiyuan Inventor before: Zeng Lihua Inventor before: Feng Man |