CN109430401A - The preparation method of mixed fermentation germination maize Yoghourt - Google Patents

The preparation method of mixed fermentation germination maize Yoghourt Download PDF

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CN109430401A
CN109430401A CN201811619598.7A CN201811619598A CN109430401A CN 109430401 A CN109430401 A CN 109430401A CN 201811619598 A CN201811619598 A CN 201811619598A CN 109430401 A CN109430401 A CN 109430401A
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germination
maize
preparation
yoghourt
water
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CN109430401B (en
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刘敬科
张爱霞
赵巍
张佳丽
刘莹莹
李少辉
任素芬
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Grain Research Institute of Hebei Academy of Agriculture and Forestry Sciences
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Grain Research Institute of Hebei Academy of Agriculture and Forestry Sciences
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1315Non-milk proteins or fats; Seeds, pulses, cereals or soja; Fatty acids, phospholipids, mono- or diglycerides or derivatives therefrom; Egg products
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00Preservation of edible seeds, e.g. cereals
    • A23B9/14Coating with a protective layer; Compositions or apparatus therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00Preservation of edible seeds, e.g. cereals
    • A23B9/16Preserving with chemicals
    • A23B9/24Preserving with chemicals in the form of liquids or solids
    • A23B9/26Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium

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Abstract

The preparation method of mixed fermentation germination maize Yoghourt, the invention belongs to the technical fields for the maize that germinates, take germination maize stone roller that polished rice and rice bran two parts is made, then polished rice is subjected to saccharification processing, rice bran mixes after pulverizing with the milk of polished rice, sterilizing after saccharification, fermenting agent is added thereto in 38-42 DEG C of fermentation 6-8h, obtains mixed fermentation germination maize Yoghourt.Invention preparation method is simple, enriches the type of Yoghourt, develops the Yoghourt of brand-new taste, enhances the nutritive value and functional characteristic of Yoghourt.

Description

The preparation method of mixed fermentation germination maize Yoghourt
Technical field
The invention belongs to the technical field for the maize that germinates, the maize that is related to germinateing is used to prepare Yoghourt, and in particular to mixing The preparation method of fermentation germination maize Yoghourt.Preparation method of the present invention is simple, enriches the type of Yoghourt, develops brand-new taste Yoghourt, enhance the nutritive value and functional characteristic of Yoghourt.
Background technique
Fermented yoghourt refers to be formulated by microbial fermentation, usually using fresh milk as raw material, is added certain The sucrose of ratio adds a kind of dairy produce made of pure lactobacillus inoculation culture after high temperature sterilization is cooling.
Yoghourt has become fashionable global fermented dairy product, and Yoghourt has enhancing digestive function, promotes appetite, enhancing intestines The function of wriggling with body substance metabolism often drinks and is conducive to promote human health, in recent years by more and more consumers It pursues and likes.It is promoted as people's eating habit changes with quality of life, novel grain class Yoghourt demand is growing.
The maize that germinates is that maize is superior to common grain through product obtained by germination technology, nutritive value and functional characteristic Rice.In germination process, a large amount of proenzymes are activated and discharge maize, drop the macromolecular substances such as starch and protein in seed Solution, generates easily digested small-molecule substance, and nutritive value is improved, and a variety of physiological activity inside maize Ingredient is enriched with, therefore germinate maize nutritive value with higher and unique health-care effect.Exploitation germination maize Yoghourt can be with Enriched acid milk category, the demand of modern life people nutrition and life.
Summary of the invention
The present invention to solve the above problems, provide a kind of preparation method of mixed fermentation germination maize Yoghourt,
The present invention be realize its purpose the technical solution adopted is that:
The preparation method of mixed fermentation germination maize Yoghourt takes germination maize stone roller that polished rice and rice bran two parts is made, then Polished rice is subjected to saccharification processing, rice bran mixes after pulverizing with the milk of polished rice, sterilizing after saccharification, mixed material is obtained, to Fermenting agent is wherein added in 38-42 DEG C of fermentation 6-8h, obtains mixed fermentation germination maize Yoghourt.The saccharification processing is will Polished rice adds 2-3 times of water to be beaten, and carbohydrase is then added and carries out saccharification processing 30-60min, the polished rice and carbohydrase in 95 DEG C Mass ratio be 10:(0.05-0.1).The mass ratio of fermenting agent and mixed material is (0.1-1): 1000.
The fermenting agent is in lactobacillus plantarum, streptococcus thermophilus, lactobacillus bulgaricus, Bifidobacterium One or more combination.
Millet after shelling is placed in germinator, Hypoxia Stress germination 21-32.5h, is germinateing under the conditions of 0-40 DEG C During, calcium chloride solution, regulator and fungicide are sprayed into germinator every 30-40min, after germination, cleaning is done It is dry, obtain germination maize.Spraying calcium chloride solution during germination can be with stratification;Spray regulator effect be into One step stratification improves bactericidal effect, avoids the loss of nutritional ingredient, and the conversion and content for improving nutritional ingredient are promoted;Spray The effect of leaching fungicide is to kill bacterial micro-organism, guarantees the environment and stability of germination, eliminates the security risk of germination maize, And for guaranteeing the humidity of maize in germination process.
(oxygen content of Hypoxia Stress is controlled in 0-15% Hypoxia Stress, does not include that 0) germination is specific under the conditions of 0-40 DEG C For, 0 DEG C of low temperature stress 0.5h, 20 DEG C of germination 4-6h, 25 DEG C of germination 4-6h, 30 DEG C of Hypoxia Stress germinate 4-6h, 35 DEG C of hypoxemia sides of body Compel germination 4-6h, 40 DEG C of Hypoxia Stress germinations 0.5-2h, 20 DEG C of germination 4-6h.First the purpose of 0 DEG C of low temperature stress 0.5h is to be promoted to resist Oxidation activity enzyme activity and alpha-aminobutyric acid content;20 DEG C of germinations 4-6h, 25 DEG C of germination 4-6h, spray promotees in this process Feed liquor and regulator, collective effect improve the activity of activation relevant enzyme, build the micro-ecological environment for being suitble to maize germination;30 DEG C low Oxdative stress germination 4-6h, it is therefore an objective to inhibit the respiration of microorganism in raw material, improve the vigor of maize amylase, accelerate starch Degradation;35 DEG C of Hypoxia Stress germination 4-6h, the anti-nutritional factors in maize can be effectively reduced by spraying regulator within this time Effect, should during a large amount of enrichment physiological activators;40 DEG C of Hypoxia Stress germination 0.5-2h, activate endogenous proteinase, increase Add the content of active peptide in germination maize;20 DEG C of germination 4-6h, after have passed through above-mentioned a series of control, enrichment is produced at this stage The nutritional activities factor of product, such as Vc, VB6, folic acid, γ-aminobutyric acid be largely enriched with and promoted.
The regulator includes, by mass percentage, lysine 0-1%, threonine 0-1%, magnesium chloride 0-1%, Vitamin B50-1%, niacin 0-1%, vitamin B20-1%, riboflavin 0-1%, Fructus Cucurbitae moschatae extract 0-1%, surplus are water, Middle lysine, threonine, magnesium chloride, vitamin B5, niacin, vitamin B2, riboflavin, Fructus Cucurbitae moschatae extract dosage be not simultaneously 0, regulator and the weight ratio of millet after shelling are (1-5): 10.The fungicide is Ozone Water and/or electrolysis water, electrolysis water It is (1-10): 1, consistency of ozone water 2-6mg/L with the volume ratio of Ozone Water, the pH value of electrolysis water is 1-12, fungicide and de- The weight ratio of millet is (1-5) after shell: 10.The promotion liquid is selected from 0-10mmol/L calcium chloride and 0-20mmol/L chlorination Sodium, calcium chloride and sodium chloride are not 0 simultaneously, and the weight ratio of millet is (0.5-5) after promoting liquid and shelling: 1.
After drying, gained germination maize is placed in aftertreatment fluid and impregnates 30-40min, then in 40-50 DEG C of air blast Dry 10-20h, the aftertreatment fluid by mass percentage, including 0.5-1 parts of tea polyphenols, 2-5 parts of xylitol, alanine 6- 12 parts, 3-6 parts of histidine, 3-6 parts of tryptophan, 1000 parts of water.Through industrial production obtain germination maize after, storage, place Stability and safety are the big problems for needing to solve, general at present using measures such as vacuum packaging, addition preservatives, above-mentioned Though measure has certain effect, the use mouthfeel of germination maize will affect, after placement using mouthfeel and nutritional ingredient Decline.For the stability and safety for solving the problems, such as storage, placing, the present invention is devised after obtaining germination maize by institute The maize that must germinate, which is placed in aftertreatment fluid, impregnates 30-40min, then then at the processing mode of 40-50 DEG C of forced air drying 10-20h, Layer protecting film is formed on germination maize surface by the processing of the design, prevents outside moisture from entering germination maize, destroys hair The state of bud maize;The nutrient loss of germination maize is prevented, germination maize is wrapped in protective film, reduces external environment Influence to germination maize.The collocation of tea polyphenols, xylitol, alanine, histidine, tryptophan, molten in aftertreatment fluid of the present invention Yu Shuihou is contacted with germination maize, is adsorbed on the surface of germination maize, and xylitol can prevent foreign particle and germination grain in environment Rice combines, and mildew, spot become caused by avoiding because of foreign particle;Alanine, histidine, tryptophan triplicity promote essential amino acid Content promoted product nutritive effect;Tea polyphenols prevent germination maize generation brown stain, generation heterochromatic, avoid influencing appearance and matter Amount;The ratio control of above-mentioned five kinds of ingredients can effectively inhibit the growth of bacterium and microorganism, improve the storage of germination maize, place Stability and safety, have promoted product nutritional quality.By the triple protection of aftertreatment fluid, germination grain has been effectively ensured Rice uses mouthfeel and nutritional ingredient after placing, and extends period of storage.
It before millet shelling, is first screened with screening machine, removes rice husk, stone, empty grain;Color sorting is carried out after shelling to remove It is miscellaneous, remove moldy kernel, heterochromatic grain, then selected millet is cleaned, when cleaning, be first rinsed with water 1-2 times, then with fermentation Rice washing water afterwards impregnates 3-5min, is then rinsed well with clear water, then with brine It 1-2 times of 0.5%, finally use clear water It rinses well.When cleaning, being first rinsed with water 1-2 all over purpose is the medicament residue for removing soil, dust and a part;Then with hair Rice washing water after ferment impregnates 3-5min, it is therefore an objective to which the pesticide and chemical substance, soaking time for adsorbing the attachment of maize surface need to control For system in 3-5min, the time is short, can not effectively remove pesticide and chemical substance, time length can make maize expansion, cause nutrition at It shunts and loses, influence subsequent germination;Then it is rinsed well with clear water, it is therefore an objective to rinse the rice washing water after fermentation above-mentioned dry Only, it prevents from breeding bacterium and microorganism;Again with brine It 1-2 times of 0.5%, it is therefore an objective to remove the worm and worm on maize Ovum, prevents that maize is infested, moth;Finally rinsed well with clear water, it is therefore an objective to remaining substance after above-mentioned cleaning is cleaned up, It avoids influencing subsequent germination process.When cleaning, each cleaning process cannot exchange or upset, if not being first rinsed with water 1-2 times, but first with after fermentation rice washing water or 0.5% brine It it will cause dust and soil are cleaning dirty, easily It is adsorbed on maize surface, breeds bacterial micro-organism, the infested moth of maize;If it is clear to carry out rice washing water after first carrying out saline solution cleaning It washes, it will cause the residuals such as pesticide and chemical substance are more, in research process, process cleaning of the invention, pesticide and chemical substance < 1ppm is remained, therefore, the process sequence for needing strict control to clean.
The beneficial effects of the present invention are:
1, mixed fermentation of the invention germination maize Yoghourt long-term drinking can improve human body constitution in weak base, make constitution PH value keep consistent with blood, so that the soda acid for reaching human body keeps balancing, and then disease infringement has been resisted, due to alkalinity Constitution is unfavorable for the growth of tumour cell, can substantially reduce the Expectancy of cancer.
2, mixed fermentation germination maize Yoghourt prepared by the present invention is full of nutrition, comprehensive, has complementary advantages, and the maize that germinates adds Enter greatly improved the nutritive value and functional characteristic of Yoghourt;Morning is edible, can detoxicating intestine;Noon is edible, can refresh oneself awake Brain;Supper can eliminate hunger with helping sleep.
3, any preservative is not added, and the stored refrigerated phase is 10-15 days.
4, it need not chew, the smashed milled rice flour of germination maize and bran powder enter in stomach, and digestion process moderate stimulation stomach is viscous Film, so that gastric mucosa be promoted to generate, the effect of playing reparation damaged gastric mucosa, reach stomach and intestine through digestion discharge more nutrition at Point, it is easier to gastro-intestinal digestion absorbs, and therefore, the addition for the maize that germinates has the function of nourishing the stomach appetizing.
5, through detecting, the beneficial bacterium quantity contained in the germination maize Yoghourt of the method for the present invention preparation is higher than commercially available similar production Product 20-30%.
6, polished rice is made and rice bran two parts are separately added into germination maize stone roller, and polished rice is through being saccharified, and rice bran only crushes not The design of saccharification enables the enzyme lytic cell wall of the microorganism secretion in fermentation, improves the producing level of nutrient;Germinate maize Addition decompose during the fermentation, the generation of internal nuisance can be inhibited, protein is decomposed in sterile fermentation process, easily In digestion and absorption.Protein content, flavones content, Vc, VB of germination maize6, folic acid, alpha-aminobutyric acid content is much higher than common Maize, this part high nutrient is remained after fermented, so that the nutritive value of Yoghourt greatly improved.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below.
One, specific embodiment
Embodiment 1
It takes germination maize stone roller that polished rice and rice bran two parts is made, polished rice is then subjected to saccharification processing, 10 portions of polished rice add 20 Then part water mashing is added 0.05 part of carbohydrase and carries out saccharification processing 30min in 95 DEG C.After rice bran ultramicro grinding and after saccharification Polished rice, sterilizing milk mixing, 0.1 part of lactobacillus plantarum is wherein added into 1000 parts of mixed materials in 38 DEG C of fermentation 8h, obtains To mixed fermentation germination maize Yoghourt.
Germination maize used can be prepared by existing maize germination technology, and more preferably mode is using preparation of the invention Method is as follows:
It is first screened with screening machine, removes rice husk, stone, empty grain;After shelling carry out color sorting removal of impurities, remove moldy kernel, Then heterochromatic grain cleans selected millet, when cleaning, be first rinsed with water 1-2 times, is then impregnated with the rice washing water after fermentation Then 3min is rinsed well with clear water, then with brine It 1-2 times of 0.5%, finally rinsed well with clear water.It will shelling Millet afterwards, which is placed in germinator, carries out germination operation, and 0 DEG C of low temperature stress 0.5h, 20 DEG C of germination 4h, 25 DEG C of germination 4h, 30 DEG C low Oxdative stress germinates 4h, and 35 DEG C of Hypoxia Stress germinate 5h, and 40 DEG C of Hypoxia Stress germinate 0.5h (oxygen content 5%), 20 DEG C of germination 4h, During germination, is sprayed every 30min into germinator and promote liquid (10mmol/L calcium chloride), regulator and Ozone Water (smelly After oxygen water concentration is 2mg/L) germination, cleaning is washed with clear water, 40 DEG C of forced air drying 20h obtain germination maize.
The regulator includes, by mass percentage, lysine 1%, threonine 1%, magnesium chloride 1%, vitamin B51%, niacin 1%, vitamin B21%, riboflavin 1%, Fructus Cucurbitae moschatae extract 1%, surplus are water.Paddy after regulator and shelling The weight ratio of son is 1:10, and the weight ratio of the millet after fungicide and shelling is 1:10;The weight of millet after promoting liquid and shelling Amount is than being 0.5:1.
Further, after forced air drying, gained germination maize is placed in aftertreatment fluid and impregnates 30min, then in 40 DEG C of forced air drying 20h, the aftertreatment fluid by mass percentage, including tea polyphenols 0.5mg, xylitol 2mg, alanine 6mg, histidine 3mg, tryptophan 3mg, water 1000mg.
The stored refrigerated phase is 13 days.
Embodiment 2
It takes germination maize stone roller that polished rice and rice bran two parts is made, takes germination maize stone roller that polished rice and rice bran two parts is made, so Polished rice is subjected to saccharification processing afterwards, 10 portions of polished rice add 30 parts of water mashing, and 0.1 part of carbohydrase is then added and carries out at saccharification in 95 DEG C Manage 60min.Rice bran mixes after pulverizing with the milk of polished rice, sterilizing after saccharification, is wherein added 1 into 1000 parts of mixed materials Part hair lactobacillus plantarum and the fermenting agent of streptococcus thermophilus compounding obtain mixed fermentation germination maize acid in 42 DEG C of fermentation 6h Milk.
Germination maize used can be prepared by existing maize germination technology, and more preferably mode is using preparation of the invention Method is as follows:
It is first screened with screening machine, removes rice husk, stone, empty grain;After shelling carry out color sorting removal of impurities, remove moldy kernel, Then heterochromatic grain cleans selected millet, when cleaning, be first rinsed with water 1-2 times, is then impregnated with the rice washing water after fermentation Then 4min is rinsed well with clear water, then with brine It 1-2 times of 0.5%, finally rinsed well with clear water.It will shelling Millet afterwards, which is placed in germinator, carries out germination operation, and 0 DEG C of low temperature stress 0.5h, 20 DEG C of germination 5h, 25 DEG C of germination 5h, 30 DEG C low Oxdative stress germinates 5h, and 35 DEG C of Hypoxia Stress germinate 6h, and 40 DEG C of Hypoxia Stress germinate 1h (oxygen content 10%), 20 DEG C of germination 5h, During germination, is sprayed every 35min into germinator and promote liquid (10mmol/L calcium chloride), regulator and electrolysis water (electricity The pH value of Xie Shui is 12), after germination, to wash cleaning with clear water, 50 DEG C of forced air drying 10h obtain germination maize.
The regulator includes, by mass percentage, lysine 0.5%, threonine 0.5%, magnesium chloride 0.5%, Vitamin B50.5%, niacin 0.5%, vitamin B20.5%, riboflavin 0.5%, Fructus Cucurbitae moschatae extract 0.5%, surplus are water.It adjusts The weight ratio of millet after saving agent and shelling is 5:10, and the weight ratio of the millet after fungicide and shelling is 5:10;Promote liquid with The weight ratio of millet after shelling is 5:1.
Further, after forced air drying, gained germination maize is placed in aftertreatment fluid and impregnates 35min, then in 50 DEG C of forced air drying 10h, the aftertreatment fluid by mass percentage, including tea polyphenols 1mg, xylitol 3mg, alanine 8mg, Histidine 4mg, tryptophan 4mg, water 1000mg.
The stored refrigerated phase is 15 days.
Embodiment 3
It takes germination maize stone roller that polished rice and rice bran two parts is made, takes germination maize stone roller that polished rice and rice bran two parts is made, so Polished rice is subjected to saccharification processing afterwards, 10 portions of polished rice add 25 parts of water mashing, and 0.08 part of carbohydrase is then added and is saccharified in 95 DEG C Handle 40min.Rice bran mixes after pulverizing with the milk of polished rice, sterilizing after saccharification, wherein adds into 1000 parts of mixed materials Enter 0.5 part of fermenting agent in 40 DEG C of fermentation 7h, obtains mixed fermentation germination maize Yoghourt.Fermenting agent by lactobacillus plantarum, The fermenting agent of lactobacillus bulgaricus, Bifidobacterium compounding.The present invention utilizes lactobacillus bulgaricus, Bifidobacterium and plant The mixed bacteria of lactobacillus produces Yoghourt, and since lactobacillus plantarum produces, acid amount is high, stability is strong, cooperates with other two kinds of bacterium Acid is bred and produced to agent in Yoghourt fermentation raised growth early period, so as to shorten the setting time of Yoghourt, improves the solidifying of Yoghourt Solid fraction state reduces whey separation.Lactobacillus plantarum can also generate a large amount of aldehydes and esters object in production fermentation process Matter so that Yoghourt tart flavour is suitable for tasty and refreshing, and has typical cultured milk's flavor outstanding;Since lactobacillus plantarum also has maintenance bacterium The function of group's balance, therefore the stability of whole strain is greatly improved during the fermentation, so that later period Yoghourt is saving It is few that slow acid, the subtle whey amount of precipitation of mouthfeel variation are risen in sales process;The sour power of the strong production of lactobacillus plantarum, not only accelerates acid The fermenting speed of milk also shortens the production cycle simultaneously, saves the cost of manpower, energy consumption, equipment etc..
Germination maize used can be prepared by existing maize germination technology, and more preferably mode is using preparation of the invention Method is as follows:
It is first screened with screening machine, removes rice husk, stone, empty grain;After shelling carry out color sorting removal of impurities, remove moldy kernel, Then heterochromatic grain cleans selected millet, when cleaning, be first rinsed with water 1-2 times, is then impregnated with the rice washing water after fermentation Then 5min is rinsed well with clear water, then with brine It 1-2 times of 0.5%, finally rinsed well with clear water.It will shelling Millet afterwards, which is placed in germinator, carries out germination operation, and 0 DEG C of low temperature stress 0.5h, 20 DEG C of germination 6h, 25 DEG C of germination 6h, 30 DEG C low Oxdative stress germinates 6h, and 35 DEG C of Hypoxia Stress germinate 6h, and 40 DEG C of Hypoxia Stress germinate 2h (oxygen content 15%), 20 DEG C of germination 6h, During germination, sprayed every 40min into germinator and promote liquid (20mmol/L sodium chloride and 10mmol/L calcium chloride)), (volume ratio of electrolysis water and Ozone Water is 5:1, and the pH value of consistency of ozone water 3mg/L, electrolysis water are for regulator and fungicide 5), after germination, cleaning is washed with clear water, 45 DEG C of forced air drying 15h obtain germination maize.
The regulator includes, by mass percentage, lysine 0.3%, threonine 0.5%, magnesium chloride 0.8%, Vitamin B50.3%, niacin 0.2%, vitamin B20.3%, riboflavin 0.1%, Fructus Cucurbitae moschatae extract 0.7%, surplus are water.It adjusts The weight ratio of millet after saving agent and shelling is 3:10, and the weight ratio of the millet after fungicide and shelling is 3:10;Promote liquid with The weight ratio of millet after shelling is 3:1.
Further, after forced air drying, gained germination maize is placed in aftertreatment fluid and impregnates 40min, then in 45 DEG C of forced air drying 15h, the aftertreatment fluid by mass percentage and, including tea polyphenols 0.8mg, xylitol 4mg, alanine 10mg, histidine 5mg, tryptophan 5mg, water 1000mg.
The stored refrigerated phase is 19 days.
Embodiment 4
It takes germination maize stone roller that polished rice and rice bran two parts is made, takes germination maize stone roller that polished rice and rice bran two parts is made, so Polished rice is subjected to saccharification processing afterwards, 10 portions of polished rice add 23 parts of water mashing, and 0.07 part of carbohydrase is then added and is saccharified in 95 DEG C Handle 50min.Rice bran mixes after pulverizing with the milk of polished rice, sterilizing after saccharification, wherein adds into 1000 parts of mixed materials Enter 0.8 part of Bifidobacterium in 39 DEG C of fermentation 6.5h, obtains mixed fermentation germination maize Yoghourt.
Germination maize used can be prepared by existing maize germination technology, and more preferably mode is using preparation of the invention Method is as follows:
It is first screened with screening machine, removes rice husk, stone, empty grain;After shelling carry out color sorting removal of impurities, remove moldy kernel, Then heterochromatic grain cleans selected millet, when cleaning, be first rinsed with water 1-2 times, is then impregnated with the rice washing water after fermentation Then 3min is rinsed well with clear water, then with brine It 1-2 times of 0.5%, finally rinsed well with clear water.It will shelling Millet afterwards, which is placed in germinator, carries out germination operation, 0 DEG C of low temperature stress 0.5h, 20 DEG C of germination 5.5h, 25 DEG C of germination 6h, and 30 DEG C Hypoxia Stress germination 5.5h, 35 DEG C of Hypoxia Stress germination 5h, 40 DEG C of Hypoxia Stress germinations 0.8h (oxygen content 8%), 20 DEG C are sent out Bud 5.5h sprays promotion liquid (20mmol/L sodium chloride), regulator into germinator every 33min and kills during germination (volume ratio of electrolysis water and Ozone Water is 8:1 to microbial inoculum, 7) pH value of consistency of ozone water 5mg/L, electrolysis water are that germination terminates Afterwards, cleaning is washed with clear water, 48 DEG C of forced air drying 18.5h obtain germination maize.
The regulator includes, by mass percentage, lysine 0.5%, threonine 0.3%, magnesium chloride 0.2%, Vitamin B50.5%, niacin 0.8%, vitamin B20.5%, riboflavin 0.7%, Fructus Cucurbitae moschatae extract 0.1%, surplus are water.It adjusts The weight ratio of millet after saving agent and shelling is 2:10, and the weight ratio of the millet after fungicide and shelling is 2:10;Promote liquid with The weight ratio of millet after shelling is 2:1.
Further, after forced air drying, gained germination maize is placed in aftertreatment fluid and impregnates 33min, then in 48 DEG C of forced air drying 18.5h, the aftertreatment fluid by mass percentage, including tea polyphenols 0.6mg, xylitol 5mg, alanine 12mg, histidine 6mg, tryptophan 6mg, water 1000mg.
The stored refrigerated phase is 12 days.
Embodiment 5
Germination maize is taken first to be dried, in order to which subsequent stone roller system is convenient, separates rice bran with polished rice easy, adopts With micro-wave vacuum, germination maize is placed in vacuum microwave dry, vacuum degree 5-15kPa, microwave dose 2-8W/g, Microwave drying process is carried out according to the Program alternation of " heating 50-60s, interval 25-30s, heating 50-60s, interval 25-30s ", Dry total time is 5-10min.Using microwave vacuum drying technology, quick, the whole heating and vacuum of microwave drying are combined The advantages that dry low temperature, rapid moisture removal, provides while facilitate for subsequent stone roller system, can not only keep the battalion of product well It forms point and color, smell, taste, shape, and the percent cracked rice of germinated rice can be reduced, reduce the loss of product active ingredient, and have Good bactericidal effect.Then it grinds and polished rice and rice bran two parts is made, polished rice is then subjected to saccharification processing, 10 portions of polished rice add 28 Then part water mashing is added 0.09 part of carbohydrase and carries out saccharification processing 45min in 95 DEG C.After rice bran ultramicro grinding and after saccharification Polished rice, sterilizing milk mixing, 0.8 part is wherein added into 1000 parts of mixed materials by lactobacillus plantarum, bulgarian milk bar The fermenting agent fermentation 7.5h that bacterium, Bifidobacterium compound, obtains mixed fermentation germination maize Yoghourt.The present invention uses plant cream bar Three kinds of bacterium, Bifidobacterium, lactobacillus bulgaricus bacterium carry out the cooperative fermentation of Yoghourt, yoghurt produced have viscosity it is high, Retentiveness is strong, be acidified afterwards, long shelf-life the characteristics of, the shelf-life can extend 3-5 days than single fermentation microbial inoculum.
Germination maize used can be prepared by existing maize germination technology, and more preferably mode is using preparation of the invention Method is as follows:
It is first screened with screening machine, removes rice husk, stone, empty grain;After shelling carry out color sorting removal of impurities, remove moldy kernel, Then heterochromatic grain cleans selected millet, when cleaning, be first rinsed with water 1-2 times, is then impregnated with the rice washing water after fermentation Then 5min is rinsed well with clear water, then with brine It 1-2 times of 0.5%, finally rinsed well with clear water.It will shelling Millet afterwards, which is placed in germinator, carries out germination operation, 0 DEG C of low temperature stress 0.5h, 20 DEG C of germination 6h, 25 DEG C of germination 4.5h, and 30 DEG C Hypoxia Stress germination 5h, 35 DEG C of Hypoxia Stress germination 4h, 40 DEG C of Hypoxia Stress germinations 1.5h (oxygen content 12%), 20 DEG C germinate 6h sprays into germinator every 38min during germination and promotes liquid (15mmol/L sodium chloride), regulator and fungicide (volume ratio of electrolysis water and Ozone Water is 3:1, and consistency of ozone water 4mg/L, the pH value of electrolysis water is 4), after germination, to use Clear water washing cleaning, 45 DEG C of forced air drying 12.5h obtain germination maize.
The regulator includes, by mass percentage, lysine 0.8%, threonine 0.5%, magnesium chloride 0.4%, Vitamin B50.8%, niacin 0.9%, vitamin B20.5%, riboflavin 0.2%, surplus are water.Paddy after regulator and shelling The weight ratio of son is 4:10, and the weight ratio of the millet after fungicide and shelling is 4:10;The weight of millet after promoting liquid and shelling Amount is than being 4:1.
Further, after forced air drying, gained germination maize is placed in aftertreatment fluid and impregnates 38min, then in 45 DEG C of forced air drying 12.5h, the aftertreatment fluid by mass percentage, including tea polyphenols 0.7mg, xylitol 3mg, alanine 9mg, histidine 4.5mg, tryptophan 4.5mg, water 1000mg.
The stored refrigerated phase is 17 days.
Embodiment 6
Take germination maize stone roller polished rice and rice bran two parts is made, polished rice is then subjected to saccharification processing, then by polished rice into Row saccharification processing, 10 portions of polished rice add 20 parts of water mashing, and 0.05 part of carbohydrase is then added and carries out saccharification processing 30min in 95 DEG C. Rice bran mixes after pulverizing with the milk of polished rice, sterilizing after saccharification, and 0.1 portion of plant is wherein added into 1000 parts of mixed materials Bacillus acidi lactici obtains mixed fermentation germination maize Yoghourt in 38 DEG C of fermentation 8h.
Germination maize used can be prepared by existing maize germination technology, and more preferably mode is using preparation of the invention Method is as follows:
Artificial selected maize seed, removes the impurity such as some miscellaneous skins and stone, takes a certain amount of maize to be placed in a beaker, first use Tap water rinses 3-5 times, washes away surface dirt, then 2 times wash with distilled water, then impregnates 30min with 0.05% sodium hypochlorite, Surface disinfection is carried out to maize, additional amount, which just floods seed, to be advisable, sodium hypochlorite is then outwelled, wash with distilled water maize, Surface sodium hypochlorite is removed, distilled water is added, room temperature condition seed soaking 8h pours out remaining water after the completion of seed soaking, and maize is equal Even be spread out in has sterilized, in the foraminate culture basket in bottom, covers antiseptic gauze, is placed in 25 DEG C of insulating boxs and carries out germination training It supports, elutes every 12h distilled water to maintain humidity.It is primary every 12h sampling, sample is placed in 40 DEG C of air dry ovens and is done Dry 12h is crushed, is sieved with 100 mesh sieve, save backup.
The stored refrigerated phase is 13 days.
Two, effect test
The raising key of present invention germination maize Yoghourt nutritive value and functional characteristic is the addition of germination maize, determines Germination maize Yoghourt nutritive value and eating effect, how to improve germination maize Yoghourt nutritive value be pursuit of the present invention Target, as decisive influence material, germinateing, maize nutrient content and ingredient are higher, are then prepared into the battalion of germination maize Yoghourt Feeding value is bigger, eating effect is better.Therefore, the nutrient content for improving germination maize is to improve germination maize Yoghourt nutritive value It is basic.By long-term exploration and research, the preparation method for analyzing germination maize plays decision to the quality of germination maize Property effect, the difference of preparation method, prepared germination maize is far from each other.
Existing preparation method compares by taking the preparation method of the embodiment of the present invention 1 as an example and in embodiment 6.Prepare the present invention Prepared germination maize (handling without aftertreatment fluid) of method is test group 1, and the prepared germination maize of the present invention (locate later by warp Manage liquid processing) it is test group 2, the prepared germination maize of maize germination technology is control group in embodiment 6, does not carry out germination treatment Maize be blank group, total ash, general flavone, Vc, VB of testing product6, folic acid, γ-aminobutyric acid, phytic acid, protein, Hu Radish cellulose content is detected, as a result such as the following table 1:
Table 1 (maize is selected from Grain Institute, Provincial Academy of Agriculture and Forestry, Hebei Provinee, Ji Gu 37)
The present invention has abandoned the operation sterilized before existing germination, such as first use sodium hypochlorite soaking sterilization, then again into Row germination, effect are to prepare for subsequent germination in order to kill the bacterium on maize surface, microorganism, but killed before germinateing Bacterium, which exists, causes part nutriment to be lost, and influences in germination process the problem of nutritional ingredient converts, the present invention by abandoning on Thought is stated, spraying fungicide during germination had both realized the effect of sterilization, while solving caused by the preceding sterilization of germination The problem of.But new problem occur: germination percentage is low, bud growth degree is short, nutriment composition transfer is unobvious, by a system The exploration and analysis of column combine work with liquid, regulator, fungicide is promoted by Hypoxia Stress germination under the conditions of 0-40 DEG C of design With, solve the problems, such as it is emerging, realize sterilization while, the conversion of nutriment and content are significantly improved, and phase 1 times or more is shortened compared with existing maize germination technology germinating time.
By above-mentioned table 1 it is found that the nutritional ingredient of the germination maize of the method for the present invention production is than existing germination maize technology institute The nutritive value of system germination maize and common maize is higher, and nutritional ingredient is significantly improved, and general flavone content is compared to general Logical maize improves 3 times or more, improves 1.2 times or more compared to existing germination maize technology;Vc content is compared to existing germination grain Rice technology, common maize are even more to realize breakthrough from zero progress;VB6Content improves 4.5 times compared to common maize More than, 2.6 times or more are improved compared to existing germination maize technology;Folate content improves 1.9 times or more compared to common maize, 1.5 times or more are improved compared to existing germination maize technology;Protein content improves 2 times or more compared to common maize, compares 1.1 times or more are improved in existing germination maize technology;Carotene carotene content improves 1.9 times or more compared to common maize, compares 1.5 times or more are improved in existing germination maize technology;Alpha-aminobutyric acid content improves 4 times or more compared to common maize, compares 2.1 times or more are improved in existing germination maize technology;Phytic acid content reduces 47% compared to common maize, compared to existing hair Bud maize technology reduces 33.8%.The germination maize of liquid processing and the germination grain handled without aftertreatment fluid after post treatment Rice place after nutrition loss situation difference it is obvious, post-treated liquid treated germination maize place 1 year after nutritional ingredient it is basic Unchanged, shelf-stability and safety are more preferable.

Claims (7)

1. the preparation method of mixed fermentation germination maize Yoghourt, which is characterized in that take germination maize stone roller that polished rice and rice bran two is made Then polished rice is carried out saccharification processing by part, rice bran mixes after pulverizing with the milk of polished rice, sterilizing after saccharification, Xiang Qi Middle addition fermenting agent obtains mixed fermentation germination maize Yoghourt in 38-42 DEG C of fermentation 6-8h.
2. the preparation method of mixed fermentation germination maize Yoghourt according to claim 1, which is characterized in that the fermentation Microbial inoculum is combined selected from one or more of lactobacillus plantarum, streptococcus thermophilus, lactobacillus bulgaricus, Bifidobacterium.
3. the preparation method of mixed fermentation germination maize Yoghourt according to claim 1, which is characterized in that after shelling Millet is placed in germinator, the Hypoxia Stress germination 21-32.5h under the conditions of 0-40 DEG C, during germination, every 30- 40min sprays calcium chloride solution, regulator and fungicide into germinator, and after germination, cleaning, drying obtain germination maize.
4. the preparation method of mixed fermentation germination maize Yoghourt according to claim 3, which is characterized in that 0-40 DEG C of condition The germination of lower Hypoxia Stress specifically, 0 DEG C of low temperature stress 0.5h, send out by 20 DEG C of germination 4-6h, 25 DEG C of germination 4-6h, 30 DEG C of Hypoxia Stress Bud 4-6h, 35 DEG C of Hypoxia Stress germinations 4-6h, 40 DEG C of Hypoxia Stress germinations 0.5-2h, 20 DEG C of germination 4-6h.
5. the preparation method of mixed fermentation germination maize Yoghourt according to claim 3, which is characterized in that the adjusting Agent includes, by mass percentage, lysine 0-1%, threonine 0-1%, magnesium chloride 0-1%, vitamin B50-1%, niacin 0-1%, vitamin B20-1%, riboflavin 0-1%, Fructus Cucurbitae moschatae extract 0-1%, surplus are water, wherein lysine, threonine, chlorine Change magnesium, vitamin B5, niacin, vitamin B2, riboflavin, Fructus Cucurbitae moschatae extract dosage not simultaneously be 0, regulator and shelling after paddy The weight ratio of son is (1-5): 10.
6. the preparation method of mixed fermentation germination maize Yoghourt according to claim 3, which is characterized in that in drying Afterwards, gained germination maize is placed in aftertreatment fluid and impregnates 30-40min, it is described then in 40-50 DEG C of forced air drying 10-20h Aftertreatment fluid by mass percentage, including 0.5-1 parts of tea polyphenols, 2-5 parts of xylitol, 6-12 parts of alanine, histidine 3-6 Part, 3-6 parts of tryptophan, 1000 parts of water.
7. the preparation method of mixed fermentation germination maize Yoghourt according to claim 3, which is characterized in that shell in millet Before, it is first screened with screening machine, removes rice husk, stone, empty grain;Color sorting removal of impurities is carried out after shelling, removes moldy kernel, heterochromatic Grain, then cleans selected millet, when cleaning, is first rinsed with water 1-2 times, then impregnates 3- with the rice washing water after fermentation Then 5min is rinsed well with clear water, then with brine It 1-2 times of 0.5%, finally rinsed well with clear water.
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