CN109418155A - A kind of breeding method of small apple bonsai - Google Patents
A kind of breeding method of small apple bonsai Download PDFInfo
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- CN109418155A CN109418155A CN201710583232.8A CN201710583232A CN109418155A CN 109418155 A CN109418155 A CN 109418155A CN 201710583232 A CN201710583232 A CN 201710583232A CN 109418155 A CN109418155 A CN 109418155A
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- root
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of breeding methods of small apple bonsai, which comprises the following steps: chooses the tissue cultivating of stock, grafts, shaping and is implanted into basin.The beneficial effects of the invention are as follows the small apple bonsai method cultivation times through the invention to greatly shorten, and survival rate of plant is high, high production efficiency, while the potted landscape compact of the production, can be placed in conventional tables, be easily managed, low cost.
Description
Technical field
The present invention relates to the production methods of potted landscape, relate more specifically to a kind of breeding method of small apple bonsai.
Background technique
Potted landscape is one of Chinese nation's excellent tradition art.It, for material, is created with plant, mountain stone, soil, water etc. by art
Make and horticultural gardening, fine view that is typical in basin, intensively moulding the Nature reach contracting dragon into skill very little, much in little
Art effect, while being poured out one's heart with scape, far-reaching artistic conception is showed, like the scenery with hills and waters scenic spot of three-dimensional beautiful scaled down version.
Potted landscape is the artistic contraction product of the landscape being presented in basin device or gardens flowers and trees landscape.Mostly with trees, flowers and plants, mountain
Stone, water, soil etc. be material, through ingenuity layout, the shape handles and meticulously maintenance, can very close spatial concentration embody mountains and rivers mind looks and
The beauty of landscape art becomes and supplies to decorate with gardens clearly rich in poetic desk, be often known as " noiseless poem, it is three-dimensional
It draws ".
Existing potted landscape is mostly large-scale potted landscape, handsome in appearance, but manages trouble, and price is more expensive, and range on probation is not extensive enough,
It cannot generally get their way into people's lives.
Summary of the invention
The purpose of the present invention is overcoming prior art defect, a kind of breeding method of small apple bonsai is provided, is passed through
The small bonsai compact of the method for the present invention production, can be placed in conventional tables, and handsome in appearance.
In order to solve the above technical problems, the invention discloses a kind of breeding methods of small apple bonsai, including stock
Tissue cultivating is grafted, is implanted into basin, shaping and cultivation；Wherein, the tissue cultivating of the stock includes following step
It is rapid:
A, clip bud point and inoculation: choosing branch vigorous apple seedling 3-5 month, and clip has been sprouted the terminal bud of branch
Or axillary bud, it peels off surfoyl and is disinfected, impregnate a quarter of an hour for example, by using 75% alcohol, then strip growth
Point is seeded on the MS culture medium of addition BA and hydrolysed casein.
B, cultivate preliminary seedling of taking root: the bud growing point being inoculated with through step a is first placed under light and cultivates 5-15 days, then in 25-
Constant temperature is protected from light culture 5-15 days at 30 DEG C；Constant temperature between cultivating, daily illumination 8-12h, luminous intensity 1000- are kept later
1500Lux has 10-15 piece bottle green blade to get preliminary seedling of taking root to height of seedling 3cm or more；Progress constant temperature is protected from light culture can
With the tissue differentiation of Expedite the application bud growing point, illumination cultivation is carried out later and is conducive to the tissue-cultured seedling progress nutrition differentiation and life
It is long.
C, it cultivates rooted seedling: the preliminary seedling of taking root is seeded on the first root media, carry out dark culture, described the
One prescription of rooting medium is that MS ties up raw+2.5ppmIBA+5.0ppm sucrose；After 7-10 days, it is enterprising to move to the second root media
Row illumination cultivation, second culture medium prescription are MS vitamin+2.5ppmIAA+5.0ppm sucrose+1.0ppmAC；Carry out light
Dark alternate culture is conducive to the preliminary seedling of taking root and carries out root tissue differentiation and growth, the life for preliminary seedling of taking root described in quickening
Root.
D, it tempers rooted seedling: taking out the rooted seedling, wash away culture medium, carry out sand culture or earth culture, pour clear water and keep
Relative humidity >=80%, 18-25 DEG C of temperature, intensity of illumination 1000-1500Lux；Seedling grows lateral root after 2-3 weeks, moves to flower
In basin, continue to pour clear water until surviving；It only pours clear water and is conducive to the healthy and strong lateral root of culture.
E, it transplants crop field: step d is cultivated to the rooted seedling survived, move to crop field, both obtain rootstock seedling after growing the 5-7 month；
The grafting carries out root grafting after being included in stem's grafting.
The root, which is grafted, in one of the embodiments, includes:
A, root is grafted: being taken out the rootstock seedling by stem's grafting and is cleaned, chooses healthy and strong lateral root clip tip, stay
The trunk of 5-10cm；Then the lateral root of mature 2-3 green apple is chosen as scion, carries out the lateral root for being grafted onto the stock
On, notch is bandaged after grafting.
B, root shaping: heap buries in shallow basin, and soil layer is made to be slightly above basin edge, and the stock grafted is placed in the soil
On heap, across on the high basin soil of heap, the native face of micro- dew will wind the progress shaping of root item using metal wire for root.
C, it cultivates: the plant after the Shape correction of root being put into cultivation canopy, is cultivated under light illumination, the intensity of illumination
For 1000-1500Lux.Illumination appropriate, which has, carries out thickening growth using the root of the grafting, convenient for forming moulding.
The stem grafting of the breeding method of the small apple bonsai includes: to repair the stem of the rootstock seedling
It cuts, stays the trunk of 10-20cm, then choose the bearing basal shoot of 2-3 green apple, clip 6-10cm is grafted as scion
Onto stock, notch is bandaged after grafting, the plant after grafting is put into cultivate in canopy and is cultivated.
Preferably, the shaping includes limb shaping and root shaping, and the limb shaping includes bud picking, priming leaf and pressure
Item；The root shaping includes cutting root, band girdle, bent root and twining root.
The beneficial effects of the invention are as follows the breeding methods of small apple bonsai of the present invention to choose apple tree as potted landscape
Plant makes its dwarfing, accelerates it by tissue cultures and breeds speed；It is grafted by root, forms it into root handsome in appearance；?
It is engaged during tissue cultures by light culture and dark culture, it is promoted to take root, improve survival rate；Tissue culture stock carries out sand culture
Later in transplanting crop field, survival rate is improved；Only high 10-20cm, made potted landscape are suitable for indoor placement to stock.In short, passing through
The small bonsai compact of the method for the present invention production, can be placed in conventional tables, be easily managed, low cost.
Specific embodiment
The present invention is described in further detail combined with specific embodiments below, to enable those skilled in the art's reference say
Bright book text can be implemented accordingly.
Embodiment 1
The breeding method of small apple bonsai, tissue cultivating including stock are grafted, are implanted into basin, shaping and cultivation
And etc.；Wherein, the stock tissue cultivating the following steps are included:
A, clip bud point and inoculation: choosing branch vigorous apple seedling 3-5 month, and clip has been sprouted the terminal bud of branch
Or axillary bud, it peels off surfoyl and is disinfected, then strip growing point, be seeded in the MS of addition BA and hydrolysed casein
On culture medium；The bud point culture medium prescription is for example are as follows: MS vitamin+3ppmBA+0.05ppm hydrolysed casein.
B, cultivate preliminary seedling of taking root: the bud growing point being inoculated with through step a is first placed under light and cultivates 5 days, then in 25-30 DEG C
Lower constant temperature is protected from light culture 5 days；Keep constant temperature between cultivating, daily illumination 12h, luminous intensity 1000Lux, to height of seedling 3cm or more, tool
There is 10-15 piece bottle green blade to get preliminary seedling of taking root；
C, it cultivates rooted seedling: the preliminary seedling of taking root is seeded on the first root media, carry out dark culture, described the
One prescription of rooting medium is that MS ties up raw+2.5ppmIBA+5.0ppm sucrose；After 10 days, moves on the second root media and carry out
Illumination cultivation, second culture medium prescription are MS vitamin+2.5ppmIAA+5.0ppm sucrose+1.0ppmAC.
D, it tempers rooted seedling: taking out the rooted seedling, wash away culture medium, carry out sand culture or earth culture, pour clear water and keep
Relative humidity >=80%, 18-25 DEG C of temperature, intensity of illumination 1500Lux；Seedling grows lateral root after 2-3 weeks, moves in flowerpot,
Continue to pour clear water until surviving.
E, it transplants crop field: step d is cultivated to the rooted seedling survived, move to crop field, both obtain rootstock seedling after growing the 5-7 month.
Later, the rootstock seedling is dug out, cleans root, carry out root grafting.It grafts the root
A, root is grafted: being taken out the rootstock seedling by stem's grafting and is cleaned, chooses healthy and strong lateral root clip tip, stay
The trunk of 10cm；Then the lateral root of mature 2-3 green apple is chosen as scion, carries out the lateral root for being grafted onto the stock
On, notch is bandaged after grafting.
B, root shaping: heap buries in shallow basin, and soil layer is made to be slightly above basin edge, and the stock grafted is placed in the soil
On heap, across on the high basin soil of heap, the native face of micro- dew will wind the progress shaping of root item using metal wire for root.
C, it cultivates: the plant after the Shape correction of root being put into cultivation canopy, is cultivated under light illumination, the intensity of illumination
For 1000-1500Lux.
After the rootstock seedling that band carried out root grafting survives, transplanting crop field carries out strong sprout, carries out stem later and transfers
It connects, stem's grafting includes: to trim the stem of the rootstock seedling, stays the trunk of 20cm, then chooses 2-3 Nian Shengping
The bearing basal shoot of fruit, clip 10cm be grafted on stock as scion, are bandaged notch after grafting, will graft
Plant afterwards is put into cultivate and be cultivated in canopy.
After stem's grafting survival, carries out limb shaping and root shaping, the limb shaping include bud picking, priming leaf and pressure
Item；The root shaping includes cutting root, band girdle, bent root and twining root.
Embodiment 2
The breeding method of small apple bonsai, tissue cultivating including stock are grafted, are implanted into basin, shaping and cultivation
And etc..
The tissue cultivating of the stock the following steps are included:
A, clip bud point and inoculation: choosing branch vigorous apple seedling 3-5 month, and clip has been sprouted the terminal bud of branch
Or axillary bud, it peels off surfoyl and is disinfected, then strip growing point, be seeded in the MS of addition BA and hydrolysed casein
On culture medium；The bud point culture medium prescription is for example are as follows: MS vitamin+3ppmBA+0.05ppm hydrolysed casein.
B, cultivate preliminary seedling of taking root: the bud growing point being inoculated with through step a is first placed under light and cultivates 10 days, then in 25-30
Constant temperature is protected from light culture 10 days at DEG C；Keep culture between constant temperature, daily illumination 10h, luminous intensity 1500Lux, to height of seedling 3cm with
On, have 10-15 piece bottle green blade to get preliminary seedling of taking root.
C, it cultivates rooted seedling: the preliminary seedling of taking root is seeded on the first root media, carry out dark culture, described the
One prescription of rooting medium is that MS ties up raw+2.5ppmIBA+5.0ppm sucrose；After 7 days, moves on the second root media and carry out
Illumination cultivation, second culture medium prescription are MS vitamin+2.5ppmIAA+5.0ppm sucrose+1.0ppmAC.
D, it tempers rooted seedling: taking out the rooted seedling, wash away culture medium, carry out sand culture or earth culture, pour clear water and keep
Relative humidity >=80%, 18-25 DEG C of temperature, intensity of illumination 1500Lux；Seedling grows lateral root after 2-3 weeks, moves in flowerpot,
Continue to pour clear water until surviving.
E, it transplants crop field: step d is cultivated to the rooted seedling survived, move to crop field, both obtain rootstock seedling after growing the 5-7 month.
Later, the rootstock seedling is dug out, cleans root, carry out root grafting.It grafts the root
A, root is grafted: being taken out the rootstock seedling by stem's grafting and is cleaned, chooses healthy and strong lateral root clip tip, stay
The trunk of 10cm；Then the lateral root of mature 2-3 green apple is chosen as scion, carries out the lateral root for being grafted onto the stock
On, notch is bandaged after grafting.
B, root shaping: heap buries in shallow basin, and soil layer is made to be slightly above basin edge, and the stock grafted is placed in the soil
On heap, across on the high basin soil of heap, the native face of micro- dew will wind the progress shaping of root item using metal wire for root.
C, it cultivates: the plant after the Shape correction of root being put into cultivation canopy, is cultivated under light illumination, the intensity of illumination
For 1000-1500Lux.
After the rootstock seedling that band carried out root grafting survives, transplanting crop field carries out strong sprout, carries out stem later and transfers
It connects, stem's grafting includes: to trim the stem of the rootstock seedling, stays the trunk of 10cm, then chooses 2-3 Nian Shengping
The bearing basal shoot of fruit, clip 6cm be grafted on stock, are bandaged notch after grafting, after grafting as scion
Plant be put into cultivate canopy in cultivated.
After stem's grafting survival, carries out limb shaping and root shaping, the limb shaping include bud picking, priming leaf and pressure
Item；The root shaping includes cutting root, band girdle, bent root and twining root.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (4)
1. a kind of breeding method of small apple bonsai, tissue cultivating including stock is grafted, is implanted into basin, shaping and training
It educates；It is characterized by: the tissue cultivating of the stock the following steps are included:
A, clip bud point and inoculation: choosing branch vigorous apple seedling 3-5 month, clip sprouted branch terminal bud or
Axillary bud is peelled off surfoyl and is disinfected, then strips growing point, and the MS culture of addition BA and hydrolysed casein is seeded in
On base；
B, cultivate preliminary seedling of taking root: the bud growing point being inoculated with through step a is first placed under light and cultivates 5-15 days, then in 25-30 DEG C
Lower constant temperature is protected from light culture 5-15 days；Constant temperature between holding culture later, daily illumination 8-12h, luminous intensity 1000-1500Lux, to
Height of seedling 3cm or more has 10-15 piece bottle green blade to get preliminary seedling of taking root；
C, it cultivates rooted seedling: the preliminary seedling of taking root is seeded on the first root media, carry out dark culture, described first is raw
Root culture medium prescription is MS vitamin+2.5ppmIBA+5.0ppm sucrose；After 7-10 days, moves on the second root media and carry out
Illumination cultivation, second culture medium prescription are MS vitamin+2.5ppmIAA+5.0ppm sucrose+1.0ppmAC；
D, it tempers rooted seedling: taking out the rooted seedling, wash away culture medium, carry out sand culture or earth culture, pour clear water and keep opposite
Humidity >=80%, 18-25 DEG C of temperature, intensity of illumination 1000-1500Lux；Seedling grows lateral root after 2-3 weeks, moves in flowerpot,
Continue to pour clear water until surviving；
E, it transplants crop field: step d is cultivated to the rooted seedling survived, move to crop field, both obtain rootstock seedling after growing the 5-7 month；It is described to transfer
It connects and carries out root grafting after being included in stem's grafting.
2. the breeding method of small apple bonsai as described in claim 1, which is characterized in that the root, which is grafted, includes:
A, root is grafted: being taken out the rootstock seedling by stem's grafting and is cleaned, chooses healthy and strong lateral root clip tip, stay 5-
The trunk of 10cm；Then the lateral root of mature 2-3 green apple is chosen as scion, carries out the lateral root for being grafted onto the stock
On, notch is bandaged after grafting；
B, root shaping: heap buries in shallow basin, and soil layer is made to be slightly above basin edge, and the stock grafted is placed in the mound
On, across on the high basin soil of heap, the native face of micro- dew will wind the progress shaping of root item using metal wire for root；
C, it cultivates: the plant after the Shape correction of root being put into cultivation canopy, is cultivated under light illumination, the intensity of illumination is
1000-1500Lux。
3. the breeding method of small apple bonsai as described in claim 1, which is characterized in that stem grafting include: by
The stem of the rootstock seedling is trimmed, and the trunk of 10-20cm is stayed, and then chooses the bearing basal shoot of 2-3 green apple, clip 6-
10cm be grafted on stock as scion, is bandaged notch after grafting, and the plant after grafting is put into cultivation canopy
In cultivated.
4. the breeding method of small apple bonsai as described in claim 1, which is characterized in that the shaping includes limb shaping
With root shaping, the limb shaping includes bud picking, priming leaf and press strip；The root shaping include cut root, band girdle, bent root with
And twine root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710583232.8A CN109418155A (en) | 2017-07-18 | 2017-07-18 | A kind of breeding method of small apple bonsai |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710583232.8A CN109418155A (en) | 2017-07-18 | 2017-07-18 | A kind of breeding method of small apple bonsai |
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| Publication Number | Publication Date |
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| CN109418155A true CN109418155A (en) | 2019-03-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710583232.8A Pending CN109418155A (en) | 2017-07-18 | 2017-07-18 | A kind of breeding method of small apple bonsai |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103621282A (en) * | 2013-11-07 | 2014-03-12 | 胡宁 | Method for manufacturing small bonsai |
| CN103621298A (en) * | 2013-12-04 | 2014-03-12 | 胡宁 | Cultivation method of small apple bonsai |
-
2017
- 2017-07-18 CN CN201710583232.8A patent/CN109418155A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103621282A (en) * | 2013-11-07 | 2014-03-12 | 胡宁 | Method for manufacturing small bonsai |
| CN103621298A (en) * | 2013-12-04 | 2014-03-12 | 胡宁 | Cultivation method of small apple bonsai |
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Application publication date: 20190305 |