CN109402218A - The disinfection experiment method of compound herbal disinfectant - Google Patents
The disinfection experiment method of compound herbal disinfectant Download PDFInfo
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- CN109402218A CN109402218A CN201811373692.9A CN201811373692A CN109402218A CN 109402218 A CN109402218 A CN 109402218A CN 201811373692 A CN201811373692 A CN 201811373692A CN 109402218 A CN109402218 A CN 109402218A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/22—Testing for sterility conditions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a kind of disinfection experiment methods of compound herbal disinfectant, for detecting the disinfecting power of compound herbal disinfectant, the component of compound herbal disinfectant includes Honegsukle flower P.E, Flos Chrysanthemi Indici extract, Folium Isatidis extract, ethyl alcohol, water and acetic acid chlorhexidine, comprising the following steps: prepares positive control solution, compound herbal disinfectant test solution, the first comparison sample liquid of the first comparative sample agent, the second comparison sample liquid of the second comparative sample agent；Be separately added into the above solution be made after the same strain that need to be detected positive control detection liquid, the detection liquid of compound herbal disinfectant test solution, the first contrasting detection liquid of the first comparison sample liquid, the second comparison sample liquid the second contrasting detection liquid；It takes each detection liquid to be added separately to be cultivated in culture medium, the viable bacteria clump count on each culture medium is compared to the disinfecting power to detect compound herbal disinfectant.
Description
Technical field
The present invention relates to disinfectant technical fields, particularly, are related to a kind of disinfection experiment method of compound herbal disinfectant.
Background technique
Herbaceous plant is the product of the distinctive Chinese medicine theory long-term practice in China, some herbaceous plant have the pyrolysis that disappears
The functions such as poison, bactericidal antiphlogistic, and herbaceous plant does not have the side effects such as chemical residual and specific drug resistance, if being configured to disinfectant
It is also good scheme, but disinfectant is individually prepared with herbaceous plant and is worked slowly, bacteriostasis rate and sterilizing rate is all relatively low, therefore need
Herbaceous plant and chemical disinfection substance are combined to develop compound herbal disinfectant, and existing sterilization method can not divide
The disinfecting power for not detecting herbaceous plant and chemical substance in compound herbal disinfectant is only capable of detecting the whole of compound disinfectant
Body disinfecting power.
Summary of the invention
The present invention provides a kind of disinfection experiment methods of compound herbal disinfectant, to solve existing herbal disinfectant
Disinfection experiment method can not detect the technology of the disinfecting power of herbaceous plant and chemical substance in compound herbal disinfectant respectively
Problem.
According to an aspect of the present invention, a kind of disinfection experiment method of compound herbal disinfectant is provided, it is multiple for detecting
The disinfecting power of square herbal disinfectant, the component of compound herbal disinfectant include Honegsukle flower P.E, Flos Chrysanthemi Indici extract, smalt
Leaf extract, ethyl alcohol, water and acetic acid chlorhexidine, comprising the following steps: extracting honeysuckle extract, Flos Chrysanthemi Indici extract, smalt
Leaf extract, ethyl alcohol and water are extracted according to Honegsukle flower P.E, Flos Chrysanthemi Indici extract, folium isatidis in compound herbal disinfectant
Object, ethyl alcohol and the identical proportion of water prepare the first comparative sample agent；Take ethyl alcohol, water and acetic acid chlorhexidine according to compound draft
Ethyl alcohol, water and the identical proportion of acetic acid chlorhexidine prepare the second comparative sample agent in disinfectant；Prepare positive control solution, compound
Herbal disinfectant test solution, the first comparison sample liquid of the first comparative sample agent, the second comparison sample liquid of the second comparative sample agent；Above molten
The detection that positive control detects liquid, compound herbal disinfectant test solution is made after being separately added into the same strain that need to be detected in liquid
Liquid, first comparison sample liquid the first contrasting detection liquid, second comparison sample liquid the second contrasting detection liquid；Each detection liquid is taken to distinguish
It is added in culture medium and is cultivated, the viable bacteria clump count on each culture medium is compared to detect that compound draft sterilizes
The disinfecting power of agent.
Further, before preparation solution, test solution and sample liquid, comprising the following steps: with 3% Tween 80 and 0.3%
Lecithin mixed liquor is made into neutralizer, is made into eluent with the phosphate buffer of neutralizer and 1% peptone；By neutralizer,
Eluent, distilled water, nutrient agar culture solution and the required utensil used carry out high pressure steam sterilization.
Further, prepare positive control solution: respectively by eluant, eluent, dilution 10 times after eluant, eluent and dilution 100
Eluant, eluent after times is as positive control solution；Prepare the test solution of compound herbal disinfectant: respectively by compound herbal disinfectant, use
Distilled water dilute 10 times after compound herbal disinfectant and use distilled water dilute 100 times after compound herbal disinfectant as answer
The test solution of square herbal disinfectant；Prepare the first contrasting detection liquid: after respectively the first comparative sample agent, 10 times being diluted with distilled water
First comparative sample agent and the first comparative sample agent after using distilled water to dilute 100 times are as the first contrasting detection liquid；Prepare second
Contrasting detection liquid: dilute by the second comparative sample agent, with the second comparative sample agent after 10 times of distilled water dilution and with distilled water respectively
The second comparative sample agent after releasing 100 times is as the second contrasting detection liquid.
Further, prepare positive control detection liquid, the detection liquid of compound herbal disinfectant, the first contrasting detection liquid and
Second contrasting detection liquid, comprising the following steps: in the test solution for preparing positive control solution, compound herbal disinfectant, the first comparison
Sample liquid and the second comparison sample liquid are separately added into the same strain that need to be detected, and make strain and solution interaction；Making respectively
Phase is made after mixing with the neutralizer of the solution and equivalent that take out equivalent at 2 minutes, 5 minutes, 10 minutes and 20 minutes
The detection liquid answered.
Further, the strain that need to be detected includes Escherichia coli, staphylococcus aureus, Candida albicans and typhoid fever bar
Bacterium.
Further, when the strain of each detection liquid inoculation is Escherichia coli, each detection liquid is taken to be added separately to cultivate
It is cultivated 48 hours at 37 DEG C after in base；Or it is each detection liquid inoculation strain be staphylococcus aureus when, take each inspection
It surveys after liquid is added separately in culture medium and is cultivated 48 hours at 37 DEG C；Or the strain of each detection liquid inoculation is Candida albicans
When bacterium, cultivated 72 hours at 25 DEG C after taking each detection liquid to be added separately in culture medium；Or each detection liquid inoculation
When strain is typhoid bacillus, each detection liquid is taken to be added separately to be placed in anaerobic box the culture 72 at 37 DEG C in culture medium small
When.
Further, further comprising the steps of: extracting honeysuckle extract, Flos Chrysanthemi Indici extract, ethyl alcohol, water and acetic acid chlorine
Determine according to identical as Honegsukle flower P.E, Flos Chrysanthemi Indici extract, ethyl alcohol, water and acetic acid chlorhexidine in compound herbal disinfectant
Proportion prepare the agent of third comparative sample；Folium Isatidis extract, ethyl alcohol, water and acetic acid chlorhexidine is taken to sterilize according to compound draft
Folium Isatidis extract, ethyl alcohol, water and the identical proportion of acetic acid chlorhexidine prepare the 4th comparative sample agent in agent；Prepare third comparison
Third comparison sample liquid, the 4th comparison sample liquid of the 4th comparative sample agent of sample agent；Being separately added into the above solution same needs to examine
The third contrasting detection liquid of third comparison sample liquid, the 4th contrasting detection liquid of the 4th comparison sample liquid are made after the strain of survey；It takes each
A detection liquid is added separately to be cultivated in culture medium.
The invention has the following advantages:
The disinfection experiment method of compound herbal disinfectant of the invention, due to Honegsukle flower P.E in compound herbal disinfectant
With the pharmacological components such as the chlorogenic acid, the cyanidenon glycosides that contain in Flos Chrysanthemi Indici extract to hemolytic streptococcus, golden yellow Portugal
The various pathogens such as grape coccus and infection of the upper respiratory tract Causative virus have stronger restraint, while there are also strengthen immunities, shield
Liver, antitumor function, the active constituent in Folium Isatidis extract is to staphylococcus aureus, diplococcus meningitidis, typhoid fever bar
Bacterium, Escherichia coli and Bacillus influenzae have inhibiting effect, and also have to japanese encephalitis virus, mumps virus, influenza virus
Inhibiting effect, and acetic acid chlorhexidine has killing effect, therefore compound herbal disinfectant to most Grain-positives and negative bacteria
In herbaceous plant and acetic acid chlorhexidine the disinfecting power of different strains is different, joined positive control by comparing
The culture medium for detecting liquid, the culture medium that joined the second contrasting detection liquid, is added the culture medium that joined the first contrasting detection liquid
Viable bacteria clump count on the culture medium of the detection liquid of compound herbal disinfectant, so that the draft measured in compound herbal disinfectant is planted
The disinfecting power of object, the disinfecting power of acetic acid chlorhexidine in compound herbal disinfectant and the synthesis of compound herbal disinfectant disappear
Malicious ability and according to the requirement of the types of spawn and disinfecting power of required disinfection to the herbaceous plant in compound herbal disinfectant
It is adjusted with the proportion of acetic acid chlorhexidine.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention.
The present invention is further detailed explanation below.
Specific embodiment
The embodiment of the present invention is described in detail below, but the present invention can by it is following limit and cover it is more
Kind different modes are implemented.
The disinfection experiment method of the compound herbal disinfectant of the present embodiment, for detecting the disinfection energy of compound herbal disinfectant
Power, the component of compound herbal disinfectant include Honegsukle flower P.E, Flos Chrysanthemi Indici extract, Folium Isatidis extract, ethyl alcohol, water and
Acetic acid chlorhexidine, comprising the following steps: extracting honeysuckle extract, Flos Chrysanthemi Indici extract, Folium Isatidis extract, ethyl alcohol and water are pressed
According to Honegsukle flower P.E in compound herbal disinfectant, Flos Chrysanthemi Indici extract, Folium Isatidis extract, ethyl alcohol and water is identical matches
Than preparing the first comparative sample agent；Take ethyl alcohol, water and acetic acid chlorhexidine according to ethyl alcohol, water and vinegar in compound herbal disinfectant
The second comparative sample agent of the sour identical proportion preparation of chlorhexidine；Prepare positive control solution, compound herbal disinfectant test solution, first pair
The first comparison sample liquid, the second comparison sample liquid of the second comparative sample agent than sample agent；Same need are separately added into the above solution
Obtained positive control detection liquid, the detection liquid of compound herbal disinfectant test solution, first compare the first of sample liquid after the strain of detection
Second contrasting detection liquid of contrasting detection liquid, the second comparison sample liquid；Each detection liquid is taken to be added separately to be trained in culture medium
It supports, the viable bacteria clump count on each culture medium is compared to the disinfecting power to detect compound herbal disinfectant.The present invention
Compound herbal disinfectant disinfection experiment method, due to Honegsukle flower P.E and Flos Chrysanthemi Indici extract in compound herbal disinfectant
In the pharmacological components such as the chlorogenic acid, the cyanidenon glycosides that contain to a variety of causes such as hemolytic streptococcus, staphylococcus aureus
Germ and infection of the upper respiratory tract Causative virus have stronger restraint, while there are also strengthen immunity, protect liver, antitumor function
Can, active constituent in Folium Isatidis extract to staphylococcus aureus, diplococcus meningitidis, typhoid bacillus, Escherichia coli with
And Bacillus influenzae has an inhibiting effect, and also has an inhibiting effect to japanese encephalitis virus, mumps virus, influenza virus, and vinegar
Sour chlorhexidine has a killing effect to most Grain-positives and negative bacteria, thus the herbaceous plant in compound herbal disinfectant and
Acetic acid chlorhexidine is different the disinfecting power of different strains, joined the culture of positive control detection liquid by comparing
Base, joined acetic acid chlorhexidine component fewer than compound herbal disinfectant the first contrasting detection liquid culture medium, joined than multiple
Square herbal disinfectant lacks the second contrasting detection liquid of Honegsukle flower P.E and Flos Chrysanthemi Indici extract and Folium Isatidis extract component
Culture medium, joined compound herbal disinfectant detection liquid culture medium on viable bacteria clump count, disappear to measure compound draft
The disinfecting power and compound draft of the acetic acid chlorhexidine in herbal disinfecting power, compound herbal disinfectant in toxic agent
The synthesis disinfecting power of disinfectant simultaneously sterilizes compound draft according to the requirement of the types of spawn and disinfecting power of required disinfection
The proportion of herbaceous plant and acetic acid chlorhexidine in agent is adjusted.Optionally, further comprising the steps of: extracting honeysuckle extracts
Object, Flos Chrysanthemi Indici extract, ethyl alcohol, water and acetic acid chlorhexidine according to Honegsukle flower P.E, mother chrysanthemum in compound herbal disinfectant
Flower extract, ethyl alcohol, water and the identical proportion of acetic acid chlorhexidine prepare the agent of third comparative sample；Take Folium Isatidis extract, ethyl alcohol,
Water and acetic acid chlorhexidine are according to identical as Folium Isatidis extract, ethyl alcohol, water and acetic acid chlorhexidine in compound herbal disinfectant
Proportion prepare the 4th comparative sample agent；Prepare the third comparison sample liquid of third comparative sample agent, the 4th comparison of the 4th comparative sample agent
Sample liquid；The third contrasting detection that third compares sample liquid is made after being separately added into the same strain that need to be detected in the above solution
4th contrasting detection liquid of liquid, the 4th comparison sample liquid；Each detection liquid is taken to be added separately to be cultivated in culture medium.
It is made into neutralizer with 3% Tween 80 and 0.3% lecithin mixed liquor, with the phosphoric acid of neutralizer and 1% peptone
Salt buffer is made into eluent；By neutralizer, eluent, distilled water, nutrient agar culture solution and the required utensil used into
Horizontal high voltage steam sterilizing.
Prepare positive control solution: respectively by the eluant, eluent after eluant, eluent, 10 times of dilution and the elution after 100 times of dilution
Agent is as positive control solution；It prepares the test solution of compound herbal disinfectant: being diluted respectively by compound herbal disinfectant, with distilled water
Compound herbal disinfectant after 10 times and the compound herbal disinfectant after using distilled water to dilute 100 times are as the disinfection of compound draft
The test solution of agent；Prepare the first contrasting detection liquid: respectively by the first comparative sample agent, with distilled water dilute 10 times after the first comparative sample
Agent and the first comparative sample agent after using distilled water to dilute 100 times are as the first contrasting detection liquid；Prepare the second contrasting detection liquid:
After diluting 100 times by the second comparative sample agent, with the second comparative sample agent after 10 times of distilled water dilution and with distilled water respectively
Second comparative sample agent is as the second contrasting detection liquid.
The strain that need to be detected includes Escherichia coli, staphylococcus aureus, Candida albicans and typhoid bacillus.
Prepare positive control detection liquid, the detection liquid of compound herbal disinfectant, the first contrasting detection liquid and the second comparison
Detect liquid, comprising the following steps: prepare positive control solution, compound herbal disinfectant test solution, first comparison sample liquid and
Second comparison sample liquid is separately added into the same strain that need to be detected, and makes strain and solution interaction；Acting on 2 minutes, 5 respectively
Corresponding detection is made with the neutralizer of equivalent after mixing for the solution that equivalent is taken out when minute, 10 minutes and 20 minutes
Liquid.
When the strain of each detection liquid inoculation is Escherichia coli, 37 after taking each detection liquid to be added separately in culture medium
It is cultivated 48 hours at DEG C；Or it is each detection liquid inoculation strain be staphylococcus aureus when, take each detection liquid to add respectively
It is cultivated 48 hours at 37 DEG C after entering into culture medium；Or the strain of each detection liquid inoculation takes each when being Candida albicans
A detection liquid is cultivated 72 hours at 25 DEG C after being added separately in culture medium；Or the strain of each detection liquid inoculation is typhoid fever
When bacillus, each detection liquid is taken to be added separately to be placed in culture medium in anaerobic box and cultivate 72 hours at 37 DEG C.
Prepare positive control detection liquid, the detection liquid of compound herbal disinfectant, the first contrasting detection liquid and the second comparison
Detect liquid, comprising the following steps: prepare positive control solution, compound herbal disinfectant test solution, first comparison sample liquid and
Second comparison sample liquid is separately added into the same strain that need to be detected, and makes strain and solution interaction；Acting on 2 minutes, 5 respectively
Corresponding detection is made with the neutralizer of equivalent after mixing for the solution that equivalent is taken out when minute, 10 minutes and 20 minutes
Liquid.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of disinfection experiment method of compound herbal disinfectant, for detecting the disinfecting power of compound herbal disinfectant, compound
The component of herbal disinfectant includes Honegsukle flower P.E, Flos Chrysanthemi Indici extract, Folium Isatidis extract, ethyl alcohol, water and acetic acid chlorine
Determine, which comprises the following steps:
Extracting honeysuckle extract, Flos Chrysanthemi Indici extract, Folium Isatidis extract, ethyl alcohol and water according to in compound herbal disinfectant
Honegsukle flower P.E, Flos Chrysanthemi Indici extract, Folium Isatidis extract, ethyl alcohol and the identical proportion of water prepare the first comparative sample agent；
Take ethyl alcohol, water and acetic acid chlorhexidine according to identical with ethyl alcohol, water and acetic acid chlorhexidine in compound herbal disinfectant
Proportion prepares the second comparative sample agent；
Prepare positive control solution, compound herbal disinfectant test solution, the first comparative sample agent first comparison sample liquid, the second comparative sample
Second comparison sample liquid of agent；
Positive control detection liquid, compound herbal disinfectant is made after being separately added into the same strain that need to be detected in the above solution
Test solution detection liquid, first comparison sample liquid the first contrasting detection liquid, second comparison sample liquid the second contrasting detection liquid；
It takes each detection liquid to be added separately to be cultivated in culture medium, the viable bacteria clump count on each culture medium is compared
To detect the disinfecting power of compound herbal disinfectant.
2. the disinfection experiment method of compound herbal disinfectant according to claim 1, which is characterized in that prepare solution, examination
Before liquid and sample liquid, comprising the following steps:
It is made into neutralizer with 3% Tween 80 and 0.3% lecithin mixed liquor, it is slow with the phosphate of neutralizer and 1% peptone
Fliud flushing is made into eluent；
Neutralizer, eluent, distilled water, nutrient agar culture solution and the required utensil used are subjected to high pressure steam sterilization.
3. the disinfection experiment method of compound herbal disinfectant according to claim 2, which is characterized in that
It prepares positive control solution: respectively making the eluant, eluent after eluant, eluent, 10 times of dilution and the eluant, eluent after 100 times of dilution
For positive control solution；
Prepare compound herbal disinfectant test solution: respectively by compound herbal disinfectant, with distilled water dilute 10 times after compound grass
This disinfectant and use distilled water dilute 100 times after compound herbal disinfectant as compound herbal disinfectant test solution；
Prepare the first contrasting detection liquid: respectively by the first comparative sample agent, with distilled water dilute 10 times after the first comparative sample agent with
And use the first comparative sample agent after 100 times of distilled water dilution as the first contrasting detection liquid；
Prepare the second contrasting detection liquid: respectively by the second comparative sample agent, with distilled water dilute 10 times after the second comparative sample agent with
And use the second comparative sample agent after 100 times of distilled water dilution as the second contrasting detection liquid.
4. the disinfection experiment method of compound herbal disinfectant according to claim 2, which is characterized in that prepare positive control
Detect liquid, the detection liquid of compound herbal disinfectant, the first contrasting detection liquid and the second contrasting detection liquid, comprising the following steps:
In the test solution for preparing positive control solution, compound herbal disinfectant, the first comparison sample liquid and the second comparison sample liquid difference
The same strain that need to be detected is added, makes strain and solution interaction；
The solution for taking out equivalent when acting on 2 minutes, 5 minutes, 10 minutes and 20 minutes respectively is mixed with the neutralizer of equivalent
Corresponding detection liquid is made after uniformly.
5. the disinfection experiment method of compound herbal disinfectant according to claim 1, which is characterized in that
The strain that need to be detected includes Escherichia coli, staphylococcus aureus, Candida albicans and typhoid bacillus.
6. the disinfection experiment method of compound herbal disinfectant according to claim 5, which is characterized in that
When the strain of each detection liquid inoculation is Escherichia coli, after taking each detection liquid to be added separately in culture medium at 37 DEG C
Culture 48 hours；Or
It is each detection liquid inoculation strain be staphylococcus aureus when, after taking each detection liquid to be added separately in culture medium
It is cultivated 48 hours at 37 DEG C；Or
When the strain of each detection liquid inoculation is Candida albicans, at 25 DEG C after taking each detection liquid to be added separately in culture medium
Lower culture 72 hours；Or
When the strain of each detection liquid inoculation is typhoid bacillus, each detection liquid is taken to be added separately to be placed on anaerobism in culture medium
It is cultivated 72 hours at 37 DEG C in case.
7. the disinfection experiment method of compound herbal disinfectant according to claim 1, which is characterized in that further include following step
It is rapid:
Extracting honeysuckle extract, Flos Chrysanthemi Indici extract, ethyl alcohol, water and acetic acid chlorhexidine according to it is golden in compound herbal disinfectant
Honeysuckle flower extract, Flos Chrysanthemi Indici extract, ethyl alcohol, water and the identical proportion of acetic acid chlorhexidine prepare the agent of third comparative sample；
Take Folium Isatidis extract, ethyl alcohol, water and acetic acid chlorhexidine according to Folium Isatidis extract, second in compound herbal disinfectant
Alcohol, water and the identical proportion of acetic acid chlorhexidine prepare the 4th comparative sample agent；
Prepare the third comparison sample liquid of third comparative sample agent, the 4th comparison sample liquid of the 4th comparative sample agent；
Be separately added into the above solution be made after the same strain that need to be detected third comparison sample liquid third contrasting detection liquid,
4th contrasting detection liquid of the 4th comparison sample liquid；
Each detection liquid is taken to be added separately to be cultivated in culture medium.
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