CN109400350A - A kind of rice straw helps rotten agent and its application - Google Patents

A kind of rice straw helps rotten agent and its application Download PDF

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Publication number
CN109400350A
CN109400350A CN201811338584.8A CN201811338584A CN109400350A CN 109400350 A CN109400350 A CN 109400350A CN 201811338584 A CN201811338584 A CN 201811338584A CN 109400350 A CN109400350 A CN 109400350A
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stalk
rotten agent
rice straw
helps
straw
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苏振成
班允赫
张卫建
李旭
王秀娟
王健
刘威
张惠文
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Institute of Applied Ecology of CAS
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/10Addition or removal of substances other than water or air to or from the material during the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Processing Of Solid Wastes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to agricultural biological technical fields, and in particular to a kind of rice straw helps rotten agent and its application.Every liter is helped in rotten agent by K2HPO43.0-7.0g (NH4)2SO43.0-7.0g, NaNO33.0-7.0g, MgSO4·7H2O 1.5‑3.0g,FeSO4·7H2O 0.05-0.55g, KCl 1.5-5.5g, Sodium Pyruvate 1.2-5.0g, yeast extract powder 1.5-5.5g, peptone 1.5-5.5g, soluble starch 1.5-5.5g, rice straw powder 5-10g, D-glucitol 1.5-5.5g, micro-mixed liquor 1-3ml, calcium solution 1-3ml.The present invention helps rotten agent without living microorganism, not will receive the antagonism of stalk indigenous microorganism, can activate the degrading activity of indigenous microorganism, and then promote the decomposition of returning to the field stalk, also not influenced by north and south areal variation, applied widely.

Description

A kind of rice straw helps rotten agent and its application
Technical field
The present invention relates to agricultural biological technical fields, and in particular to a kind of rice straw helps rotten agent and its application.
Background technique
China is Rice Production big country, has a large amount of rice straws idle every year or burns, not only waste of resource, also results in Serious air pollution.Straw-returning can effectively increase soil organic matter content, increase soil fertility and improve soil texture, The recycling and problem of environmental pollution of agricultural waste gurry are also solved to a certain extent.But rice straw is rich in cellulose, half Cellulose and lignin, decomposition is slow after returning to the field processing and influences the growth and farming of second stubble crop, therefore, straw-returning Mode is not easy to be received by wide farmers, and it is also very difficult that government promotes returning to the field technology.
Straw decomposing microbial inoculum can accelerate the decomposition of returning to the field stalk, be popularization and the out tape of straw-returning mode to hope. Straw decomposing microbial inoculum in the market is mostly the composite microbial system as made of several pure bacterium compoundings, and strain source is different, between strain Synergistic action effect is not good enough, and the effect is unsatisfactory for straw decomposition after spreading fertilizer over the fields in returning to the field stalk.In recent years, many researchs are with corruption Rotten stalk, the various samples such as fallen leaves, rotten wood, animal wastes, compost, sawdust, edible fungus culturing leftover bits and pieces, soil of rotting are bacterium Source has obtained multiple groups straw degradative fungus strain through domestication and enrichment culture, this kind of fungus strain forms strain using stalk as target degradation product It in long-term natural selection and evolutionary process, can be mutually adapted, coexist with peacefully, coevolution looks for that in degrading straw process A may also cooperate with plays a role, thus, straw degradative effect is also preferable.
But stalk itself also carries a variety of indigenous microorganisms, it is external when straw decomposing microbial inoculum to be applied in stalk Microbial inoculum composition bacterium will receive in stalk and soil the antagonism of indigenous bacterium, cannot quickly breed, or even be suppressed, together The decomposing microbial inoculum of reason, southern source bacterial strain composition may be not suitable with the soil environment in northern farmland, thus the stalk of decomposing microbial inoculum Degradation is difficult to play, and eventually leading to stalk cannot quick decomposition.In soil and stalk itself carries a variety of indigenous microorganisms In be also no lack of straw degradative bacterium, how to try to activate indigenous straw degradative bacterium, make up the deficiency of decomposing microbial inoculum, promote the micro- life of original inhabitants Object plays straw degradative effect, and straw-returning studies urgent need to solve the problem at present.
Summary of the invention
It is an object of that present invention to provide a kind of rice straws to help rotten agent and its application.
To achieve the above object, the invention adopts a technical scheme as:
A kind of rice straw helps rotten agent, and every liter is helped in rotten agent by K2HPO43.0-7.0g (NH4)2SO43.0-7.0g NaNO33.0-7.0g, MgSO4·7H2O 1.5-3.0g,FeSO4·7H2O 0.05-0.55g, KCl 1.5-5.5g, Sodium Pyruvate 1.2-5.0g, yeast extract powder 1.5-5.5g, peptone 1.5-5.5g, soluble starch 1.5-5.5g, rice straw powder 5- 10g, D-glucitol 1.5-5.5g, micro-mixed liquor 1-3ml, calcium solution 1-3ml.
The micro-mixed liquor is formulated following (g/L): ZnSO4·7H2O 3.5-5.5g, CoCl2·6H2O 0.50- 3.50g Na2MoO4·2H2O 0.50-3.50g, MnSO4·H2O 7.0-12.0g, CuSO4·5H2O 0.35-0.75g, H3BO3 0.3-1.20g, NiCl2·6H2O 0.15-0.55g, KI 0.15-0.55g.
The calcium solution is CaCl2Aqueous solution, concentration 10-20%.
The rice straw powder is that stalk spontaneously dries and smashes it through 40 meshes.
Rice straw helps the application of rotten agent, described that rotten agent is helped to be applied to stalk in the application as decomposition.
According to every 100 kilograms of rice straws spray it is described help rotten agent 30-100L, stalk is turned over after spraying and is buried in the earth, straw The soil that 5-10cm thickness is covered above stalk is poured water and is flooded or not Irrigation levels.
The beneficial effects of the present invention are:
Returning to the field is buried original inhabitants micro- life of the middle rice straw in the indigenous microorganism and soil that stalk itself carries at this stage Under object collective effect, stalk can be gradually degraded, it is important to be that the straw degradative bacterium as present in indigenous microorganism has played Effect, but at this stage other than carrying bacterial strain by stalk itself and being degraded, most of additional straw decomposing microbial inoculums are additional Microbial inoculum may accelerate the decomposition of returning to the field stalk due to the antagonism by indigenous microorganism, also may not act on.And then this The stalk that invention provides helps rotten agent, can promote the decomposition of returning to the field stalk, compared with decomposing microbial inoculum, helps rotten agent without the micro- life of living body Object, not will receive the antagonism of stalk indigenous microorganism, and can activate the degrading activity of stalk indigenous microorganism itself, into And promote the decomposition of returning to the field stalk, and also do not influenced by north and south areal variation, it is applied widely;Stalk of the present invention helps rotten agent to contain simultaneously Have carbon source, nitrogen source necessary to microbial growth, microelement and vitamin etc. (being contained in yeast extract), adjustment carbon-nitrogen ratio is 20-30:1 increases nitrogen source content, is unfavorable for microorganism growth to avoid nitrogen source is consumed when rice plant growth, increases simultaneously Mn necessary to one of lignin-degrading enzymes manganese peroxidase is added2+(MnSO4·H2O) to promote to be most difficult to degradation in stalk The decomposition of lignin.And used medium of the present invention is eutrophy, and each component content that forms is higher than oligotrophic culture medium, energy Nutrition is persistently enough provided for the growth and breeding of indigenous microorganism, and then plays straw degradative effect.Due to provided by the present invention It helps rotten agent to have and similarly promotes straw decomposition effect with straw decomposing microbial inoculum, and its preparation cost is low, stability is more preferable, is applicable in Range is wider, thus can substitute commercially available straw decomposing microbial inoculum, has better market prospects.
Detailed description of the invention
Fig. 1 is the disparity map that rice straw weight-loss ratio changes over time.
Fig. 2 disparity map that rice straw weight-loss ratio changes over time between different disposal provided in an embodiment of the present invention.
Fig. 3 is the drop of hemicellulose, cellulose and lignin in different disposal rice straw provided in an embodiment of the present invention Solution rule figure.
Fig. 4 is sterilizing provided in an embodiment of the present invention and the influence diagram that non-sterilization treatment degrades to rice straw.
Fig. 5 sprays the influence diagram for helping rotten agent and decomposing microbial inoculum to degrade rice straw to be provided in an embodiment of the present invention.
Specific embodiment
A specific embodiment of the invention is described further below in conjunction with example, it is noted that retouch in this place The specific embodiment stated is simply to illustrate that with the present invention is explained, it is not limited to the present invention.
The present invention helps rotten agent without living microorganism, not will receive the antagonism of stalk indigenous microorganism, can activate The degrading activity of indigenous microorganism, and then promote the decomposition of returning to the field stalk, it is not also influenced by north and south areal variation, the scope of application Extensively.
Embodiment 1
The influence that straw decomposing microbial inoculum and the present invention help rotten agent to degrade returning to the field rice straw:
1 materials and methods
1.1 test material
For examination soil collection in Shenyang Inst. of Applied Ecology, Chinese Academy of Sciences, ecological experiment station, Shenyang (41 ° of 32 ' N, 123 ° 23 ' E), mean sea level 31m, annual precipitation 700mm, frost-free period 147-164d, In The Soils are damp brown earth, and test is used Ground original position topsoil (0-20cm).Pedotheque is rejected into chad and root system of plant, it is spare after crossing 2mm sieve.
Shenyang Inst. of Applied Ecology, Chinese Academy of Sciences, ecological experiment station, Shenyang, water are collected in stalk in October, 2016 for examination Rice varieties are U.S. cutting edge of a knife or a sword 9.After rice harves, its aerial part is acquired, is ground into the segment of 2-3cm long, it is spare.For trying rice straw The basic chemical composition of stalk is shown in Table 1.
It is Tai Gu-straw decomposing of commercially available Hunan Taigu Biotechnology Co., Ltd. production for examination straw decomposing microbial inoculum Microbial inoculum (soluble powder) when spraying, weighs 5 grams of bacterium powder, is dissolved in 500ml sterile water, after mixing evenly, then with 5L water dilute It is spare.
Straw decomposing microbial inoculum stalk helps rotten agent prescription: K2HPO43.5g, (NH4)2SO43.5g, NaNO33.5g, MgSO4· 7H2O 2.5g,FeSO4·7H2O 0.5g, KCl 3.5g, Sodium Pyruvate 3.2g, yeast extract powder 3.5g, peptone 3.5g, can Soluble starch 3.5g, rice straw powder 8.0g, D-glucitol 3.5g, micro-mixed liquor 3ml, calcium solution 3ml, through deionization Water is settled to 1L.
Rotten agent micro-mixed liquor is helped to be formulated following (g/L): ZnSO4·7H2O 5.5g, CoCl2·6H2O 3.50g, Na2MoO4·2H2O3.50g, MnSO4·H2O 12.0g, CuSO4·5H2O 0.75g, H3BO31.20g NiCl2·6H2O 0.55g, KI0.55g are settled to 1L through deionized water.
Calcium solution is CaCl2Aqueous solution, concentration 10%.
The basic chemical composition of 1. rice straw of table
1.2 experimental design
This test was carried out spring in 2017, in the cell that length and width is 40 × 15m, with the distribution of " W " shape, the five of " W " A vertex, respectively marks off the test sample prescription that 1 length and width is 1.5 × 1.5m, and sample prescription depth is 0.25m.
Test totally 6 processing, two of them are control;Each test process is respectively A1: straw decomposing microbial inoculum+soil+stalk; A2: straw decomposing microbial inoculum+stalk;B1: help rotten agent+soil+stalk;B2: help rotten agent+stalk;CK1: water+soil+stalk;CK2: water+straw Stalk, 5 repetitions of each processing.
1200.00g rice straw is weighed, is divided into 6 parts, every part of 200.00g.As described above, every part of stalk is according to correspondence Processing sprays straw decomposing microbial inoculum that volume is 150ml, helps rotten agent or water, after puddling uniformly, then by quality is divided into 20 parts. Wherein A1, B1, CK1It is mixed into 2L respectively for trying soil, after stirring and evenly mixing, loading length and width is 28 × 20cm, and aperture is the nylon of 2mm In mesh bag.A2, B2, CK2Then it is directly loadable into bag.After nylon net bag is sealed, it is put into test sample prescription vertically, in each sample prescription Each processing is put into 4 mesh bags (5 sampling time points) totally, after being filled around mesh bag with soil, covers the thick soil of 5cm above Earth.
During test, experimental plot carries out conventional cultivation management.After on-test, each processing respectively the 1st, 2,3, 5, it samples 1 time within 12 months, takes out nylon net bag, reinstate clear water together with mesh bag one and rinse, until the water of outflow becomes limpid, 105 DEG C drying to constant weight.
1.3 experimental method
1.3.1 rice straw is with respect to degradation rate
Accurate weighing sample dry weight calculates opposite degradation rate (relation degradation rate, RDR).
RDR=(M0-Mt)/M0× 100%.Wherein: M0For the initial dry material quality of rice straw;MtFor dry material after processing Quality.
1.3.2 cellulose, hemicellulose and lignocellulosic measurement
With ANKOM 2000i Full-automatic fiber element analyzer (Beijing An Keborui Science and Technology Ltd.), with reference to Fan Shi method, The content of cellulose, hemicellulose and lignin in test sample.
2 results and analysis
Influence of the 2.1 returning to the field modes to rice straw degradation effect
Different disposal Rice under Condition stalk weight-loss ratio situation of change is as shown in Figure 1, as seen from the figure, and 5 months before the test, The straw degradative rate of not mixed soil processing is consistently higher than mixed soil processing 4.5%-11.6%, and only at 1st month, difference was not significant. Each processing rice straw degradation process can be divided into fast degradation phase (0-2 months) and slow degradative phase (3-12 months).? 0-2 months, each to handle rice straw degradation very fast, and when test to second month, stalk weight-loss ratio is more than 50%, second Straw degradative enters lag phase after a month, and not mixed soil processing stalk weight-loss ratio gradually tends towards stability, and mixed soil processing stalk is weightless Rate is in slow ascendant trend and cannot not surpass in reverse mixedly soil processing gradually after 5th month always.
As shown in Fig. 1 (a), after being inoculated with straw decomposing microbial inoculum, at the 1st, 2,3,5,12 month, the stalk of not mixed soil processing was lost Rate is respectively 26.8%, 61.9%, 69.8%, 72.7% and 70.2% again, and uniformly the stalk weight-loss ratio of mixed soil processing is respectively 22.3%, 50.3%, 58.9%, 66.7% and 83.2%.
In 2-5 months, not mixed soil processing is higher than the stalk weight-loss ratio of uniformly mixed soil processing
4.5%-11.6%, and significant difference, until 12nd month, uniformly the stalk weight-loss ratio of mixed soil processing is significantly higher than ratio Not mixed soil processing is high.In not mixed soil processing, the 12nd month straw degradative rate is more slightly lower than 5th month, but difference is not significant.
By Fig. 1 (b) it is found that spraying after stalk helps rotten agent, the stalk weight-loss ratio of not mixed soil processing is at the 1st, 2,3,5,12 The moon is respectively 31.5%, 62.6%, 70.4%, 75.7% and 81.0%, and the stalk weight-loss ratio that uniformly mixed soil is handled is respectively 21.5%, 52.9%, 60.5%, 68.0% and 85.9%, in 1-5 months, straw of the not mixed soil processing than uniformly mixed soil processing The high 7.7%-10.0% of stalk weight-loss ratio, and significant difference.To 12nd month, uniformly the stalk weight-loss ratio of mixed soil processing was than not mixed soil Handle high by 4.9%, also significant difference.It is different from the processing of above-mentioned decomposing microbial inoculum, after spraying and helping rotten agent, since 1st month, Even mixed soil handles and has showed significant difference not mixed soil processing, and in heteropolar significantly (the P < of the 2nd, the 3rd monthly error 0.01, similarly hereinafter).
In control group (such as Fig. 1 (c)), stalk weight-loss ratio also shows trend same as test group, the 3rd, the 5th and It 12nd month, mixes native significant with not mixed native processing difference.
2.2 decomposing microbial inoculums and help rotten agent on rice straw degradation effect influence
As shown in Fig. 2, still not mixed soil processing, rice straw are spraying decomposing microbial inoculum or are helping rotten agent for either mixed soil processing Afterwards, the stalk weight-loss ratio at 1st month is above control group, and difference is extremely significant.The stalk weight-loss ratio point of mixed soil processing group Do not reach 22.3% and 21.5%, it is higher than control group by 11.0% and 10.2% respectively, and decomposing microbial inoculum and help 2 processing groups of rotten agent Between stalk weight-loss ratio it is better than control effect without significant difference, but, illustrate to help rotten agent and commercially available decomposing microbial inoculum to have equally Promotion degradation, and helping rotten agent is mainly to be made of chemical reagent, is free of viable bacteria, thus quality is more stable, the scope of application It is wider.
Since 2nd month, different disposal group showed different degradation rules.As shown in Fig. 2 (i), in mixed soil processing In, it sprays decomposing microbial inoculum and helps the stalk weight-loss ratio of rotten agent processing group compared with the control group, without significant difference.And in not mixed soil In processing, as shown in Fig. 2 (ii), at 2-5 months, the stalk weight-loss ratio for spraying decomposing microbial inoculum group was all remarkably higher than control Group, difference are gradually reduced between 4.3%-7.1%, and with Return extension.It sprays and helps rotten agent compared with the control group, straw Stalk weight-loss ratio 2-12 it is monthly there were significant differences, difference 7.3%-9.4%.
The degradation rule of cellulose, hemicellulose and lignin during 2.3 straw degradatives
Under conditions of spraying decomposing microbial inoculum and helping rotten agent, the drop of hemicellulose, cellulose and lignin in rice straw Solution rate extends (such as Fig. 3) in rising trend with degradation time.
When test was to 1 month, the content point of decomposing microbial inoculum processing rice straw hemicellulose, cellulose and lignin is sprayed 23.8%, 7.0% and 1.9% is not reduced, it can be seen that, hemicellulose was most fast ingredient of degrading, lignin at 1st month It degrades most slow.When to 2nd month, the degradation rate of hemicellulose, cellulose and lignin respectively reaches 63.8%, 54.6% and 5.2%, compared to 1st month, the 2nd month hemicellulose, the degradation amplitude of cellulose were larger, and the degradation rate of cellulose Higher than hemicellulose, and lignin degradation is still slow (difference is not significant).When to 5th month, hemicellulose, cellulose and wood Quality degrades 74.0%, 77.8% and 34.3% respectively, and the degradation in this stage, lignin is most fast, the degradation rate of " three elements " Sequence are as follows: lignin > cellulose > hemicellulose.
It sprays and helps rotten agent processing at 1st month, the degradation rate of hemicellulose, cellulose and lignin is respectively 26.5%, 10.9% and 0%, hemicellulose is still the most fast ingredient of degradation.When to 2nd month, hemicellulose, cellulose and wood The degradation rate of quality respectively reaches 63.2%, 53.9% and 14.1%, from spray decomposing microbial inoculum processing unlike, with the 1st The moon is compared, and the degradation rate of " three elements " is significantly increased, and cellulose is also the most fast person that degrades.When to 5th month, hemicellulose, Cellulose and Lignin degradation rate are respectively increased to 79.1%, 80.2% and 34.7%, lignin be equally this stage most Fast degradation ingredient.It can be seen that either spraying decomposing microbial inoculum processing, still spray and rotten agent is helped to handle, the of straw degradative One stage (first month) hemicellulose degradation is most fast, and second stage (second month) is that cellulose degradation is most fast, the phase III (3-5 months) are that lignin degradation is most fast.
Embodiment 2
Spray the influence for helping rotten agent to sterilizing rice straw and the rice straw degradation effect that do not sterilize
1 materials and methods
1.1 test material
Shenyang Inst. of Applied Ecology, Chinese Academy of Sciences, ecological experiment station, Shenyang is collected in October, 2017 for examination stalk, Rice varieties are U.S. cutting edge of a knife or a sword 9.After rice harves, its aerial part is acquired, is ground into the segment of 2-3cm long, it is spare.
It is Tai Gu-straw decomposing of commercially available Hunan Taigu Biotechnology Co., Ltd. production for examination straw decomposing microbial inoculum Microbial inoculum (soluble powder) when spraying, weighs 5 grams of bacterium powder, is dissolved in 500ml sterile water, after mixing evenly, then with 5L water dilute It is spare.Stalk helps rotten agent prescription: K2HPO43.5g, (NH4)2SO43.5g, NaNO33.5g, MgSO4·7H2O 2.5g,FeSO4· 7H2O 0.5g, KCl 3.5g, Sodium Pyruvate 3.2g, yeast extract powder 3.5g, peptone 3.5g, soluble starch 3.5g, rice Straw powder 8.0g, D-glucitol 3.5g, micro-mixed liquor 3ml, calcium solution 3ml are settled to 1L through water.
Rotten agent micro-mixed liquor is helped to be formulated following (g/L): ZnSO4·7H2O 5.5g, CoCl2·6H2O 3.50g, Na2MoO4·2H2O3.50g, MnSO4·H2O 12.0g, CuSO4·5H2O 0.75g, H3BO31.20g NiCl2·6H2O 0.55g, KI0.55g are settled to 1L through water.
Calcium solution is CaCl2Aqueous solution, concentration 10%.
1.2 experimental design
Test totally 6 processing, respectively A1: straw decomposing microbial inoculum+stalk;A2: straw decomposing microbial inoculum+sterilizing stalk;B1: Help rotten agent+stalk;B2: help rotten agent+sterilizing stalk;CK1: water+stalk;CK2: water+sterilizing stalk, 4 repetitions of each processing.
Weigh the rice straw 50g that do not sterilize, pour into the big measuring cup of sterile 5L, measure sterile water 17ml be added 50ml cone bottom from In heart pipe, it is inserted into the nozzle of related suction pipe, the stalk into measuring cup sprays sterile water, puddles in spray, then will be in measuring cup In the nylon net bag that all stalks are transferred to 28cm × 20cm, aperture is 2mm, same method handles parallel sample, same method It sprays and helps rotten agent or straw decomposing microbial inoculum, same method processing sterilizing stalk, totally 6 processing.
This test is carried out in May, 2018, in the cell that length and width is 40 × 15m, chooses line-spacing uniformly separated 3 Ridge, after nylon net bag is sealed, level is put into furrow, covers the soil of 5cm-15cm thickness above.It is successively buried in every ridge Enter six kinds of each four repetitions of processing.
During test, experimental plot carries out normal cultivation management.After on-test, 1 was sampled at the 1st, 2,3 month respectively It is secondary, all mesh bags of a furrow are taken out every time, clear water is reinstated together in nylon net bag one and rinses, until the water flowed out in mesh bag Until becoming limpid, 105 DEG C drying to constant weight.
1.3 rice straws are with respect to degradation rate
Sample dry weight obtained by accurate weighing calculates opposite degradation rate (relation degradation rate, RDR).
RDR=(M0-Mt)/M0× 100%.Wherein: M0For the initial dry material quality of rice straw;MtFor dry material after processing Quality.
2 results and analysis
From fig. 4, it can be seen that the degradation rate of unsterilised stalk is all remarkably higher than sterilizing after spraying water, helping rotten agent and decomposing microbial inoculum Stalk.At 1st month, the degradation rate of unsterilised stalk can reach 25% or more;2nd month, it can reach 40% or more;The 3 monthly to reach about 60%.Stalk sterilize since self-contained microorganism includes that straw degradative microorganism is killed, is embedded to It could start after needing to wait microorganism in ambient enviroment to be migrated on the stalk into mesh bag with soil particle or water after in soil Degradation behavior, thus, it in time can be than the stalk lag a period of time that do not sterilize.
As seen from Figure 5, do not sterilize stalk degradation rate after spraying water, helping rotten agent and decomposing microbial inoculum the 1st, 2 months when Difference is not significant between three processing;When to 3rd month, sprays and the degradation rate of rotten agent processing is helped to be significantly higher than control;And it sprays It helps rotten agent and sprays decomposing microbial inoculum and spray degradation rate between water and the processing for spraying decomposing microbial inoculum without significant difference.Thus may be used See the degradation for spraying and rotten agent being helped to promote rice straw in third month.

Claims (6)

1. a kind of rice straw helps rotten agent, which is characterized in that every liter is helped in rotten agent by K2HPO43.0-7.0g (NH4)2SO43.0- 7.0g, NaNO33.0-7.0g, MgSO4·7H2O 1.5-3.0g,FeSO4·7H2O 0.05-0.55g, KCl 1.5-5.5g, third Ketone acid sodium 1.2-5.0g, yeast extract powder 1.5-5.5g, peptone 1.5-5.5g, soluble starch 1.5-5.5g, rice straw Powder 5-10g, D-glucitol 1.5-5.5g, micro-mixed liquor 1-3ml, calcium solution 1-3ml.
2. rice straw according to claim 1 helps rotten agent, it is characterised in that: the micro-mixed liquor formula is as follows (g/L): ZnSO4·7H2O 3.5-5.5g, CoCl2·6H2O 0.50-3.50g, Na2MoO4·2H2O 0.50-3.50g, MnSO4·H2O 7.0-12.0g, CuSO4·5H2O 0.35-0.75g, H3BO30.3-1.20g, NiCl2·6H2O 0.15- 0.55g, KI 0.15-0.55g.
3. rice straw according to claim 1 helps rotten agent, it is characterised in that: the calcium solution is CaCl2Aqueous solution, it is dense Degree is 10-20%.
4. rice straw according to claim 1 helps rotten agent, it is characterised in that: the rice straw powder is stalk natural drying And smash it through 40 meshes.
5. the application that rice straw according to claim 1 helps rotten agent, it is characterised in that: described to help rotten agent to be applied to stalk to exist Application as decomposition.
6. the application that rice straw as described in claim 5 helps rotten agent, it is characterised in that: according to every 100 kilograms of rice straws Rotten agent 30-100L is helped described in spraying, stalk is turned over after spraying and is buried in the earth, the soil of 5-10cm thickness is covered above stalk, is poured water It floods or not Irrigation levels.
CN201811338584.8A 2018-11-12 2018-11-12 A kind of rice straw helps rotten agent and its application Pending CN109400350A (en)

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AU2187795A (en) * 1994-03-30 1995-10-23 Product Services Co. Process and material for bioremediation of hydrocarbon contaminated soils
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CN101225002A (en) * 2008-01-30 2008-07-23 山东省农业科学院土壤肥料研究所 Neurotrophic agent accelerating straw quick decomposition as well as preparation method and use thereof
CN101456762A (en) * 2008-12-16 2009-06-17 广东省农业科学院土壤肥料研究所 Compost cure accelerator as well as preparation method and use thereof
CN102424808A (en) * 2011-12-26 2012-04-25 南开大学 Straw-degrading composite microbial inoculum and application thereof in pretreatment of ethanol production
CN102612910A (en) * 2012-03-20 2012-08-01 山东省农业科学院农业资源与环境研究所 Method for fertilizing winter wheat by mixed basal application of nitrogen fertilizer and maize straw
KR20150007041A (en) * 2013-07-10 2015-01-20 주식회사 식목일 The plant growth regulator using foresttree waste
CN104350991A (en) * 2014-11-21 2015-02-18 安徽喜洋洋农业科技有限公司 Semi-no-tillage machine ditching and broadcast sowing cultivation method of wheat following rice
CN105777423A (en) * 2016-02-05 2016-07-20 中科院广州能源所盱眙凹土研发中心 Preparation method for preparing aquaculture/crop planting bio-organic fertilizer through fermentation of straw and attapulgite
CN107382398A (en) * 2017-08-28 2017-11-24 广西沙田仙人滩农业投资有限公司 A kind of fertilizer decomposition agent and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1080272A (en) * 1992-06-16 1994-01-05 文登市土壤肥料工作站 Compost catalytic decomposing agent
AU2187795A (en) * 1994-03-30 1995-10-23 Product Services Co. Process and material for bioremediation of hydrocarbon contaminated soils
CN1254697A (en) * 1999-11-08 2000-05-31 高明市绿宝植物营养制品有限公司 Microbe preparation for decaying straw or stalk of crops and its preparing process
CN101225002A (en) * 2008-01-30 2008-07-23 山东省农业科学院土壤肥料研究所 Neurotrophic agent accelerating straw quick decomposition as well as preparation method and use thereof
CN101456762A (en) * 2008-12-16 2009-06-17 广东省农业科学院土壤肥料研究所 Compost cure accelerator as well as preparation method and use thereof
CN102424808A (en) * 2011-12-26 2012-04-25 南开大学 Straw-degrading composite microbial inoculum and application thereof in pretreatment of ethanol production
CN102612910A (en) * 2012-03-20 2012-08-01 山东省农业科学院农业资源与环境研究所 Method for fertilizing winter wheat by mixed basal application of nitrogen fertilizer and maize straw
KR20150007041A (en) * 2013-07-10 2015-01-20 주식회사 식목일 The plant growth regulator using foresttree waste
CN104350991A (en) * 2014-11-21 2015-02-18 安徽喜洋洋农业科技有限公司 Semi-no-tillage machine ditching and broadcast sowing cultivation method of wheat following rice
CN105777423A (en) * 2016-02-05 2016-07-20 中科院广州能源所盱眙凹土研发中心 Preparation method for preparing aquaculture/crop planting bio-organic fertilizer through fermentation of straw and attapulgite
CN107382398A (en) * 2017-08-28 2017-11-24 广西沙田仙人滩农业投资有限公司 A kind of fertilizer decomposition agent and preparation method thereof

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Application publication date: 20190301