CN109395061A - A kind of composite membrane for the nursing of skin lesions of pemphigus vulgaris position - Google Patents
A kind of composite membrane for the nursing of skin lesions of pemphigus vulgaris position Download PDFInfo
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Abstract
The present invention provides a kind of composite membranes for the nursing of skin lesions of pemphigus vulgaris position, which is prepared by wild marjoram cellulose, fibroin albumen, chitosan, the preparation method comprises the following steps: the drying herb of Chinese medicine wild marjoram is extracted with water；Extracting solution is filtered and collects, filter residue prepares cellulose；With [BMIM] Cl ion liquid dissolving wild marjoram cellulose and fibroin albumen, wild marjoram cellulose and silk fibroin composite membrane are prepared；Chitosan function solution is prepared with wild marjoram extracting solution dissolution chitosan again；Wild marjoram cellulose and silk fibroin composite membrane are soaked in chitosan function solution, takes out and air-dries to obtain the final product.The composite membrane is made using wild marjoram cellulose, fibroin albumen and chitosan by raw material, and biocompatibility is good, has the function of enhancing immune, antibacterial, nutrition skin, can be used for the nursing at skin lesions of pemphigus vulgaris position.
Description
Technical field
The present invention relates to medical instruments fields, and in particular to a kind of for the compound of skin lesions of pemphigus vulgaris position nursing
Film.The composite membrane is made using wild marjoram cellulose, fibroin albumen and chitosan by raw material, and biocompatibility is good, and there is enhancing to exempt from
Epidemic disease, antibacterial, nutrition skin function, can be used for the nursing at skin lesions of pemphigus vulgaris position.
Background technique
Pemphigus is a kind of chronic and serious, the skin disease with recurrent.The cause of disease of the disease is not yet completely clear, at present
It is considered as a kind of autoimmune disease.Pemphigus can be generally divided into four types, be respectively: vulgaris, proliferous type, fallen leaves
Type, erythema type.In all pemphigus patients, Pemphigus Vulgaris accounts for about 70%, after disease development to a certain extent
Patient skin will appear the soya bean blister big to walnut, and blister wall is thin and fragile, and flush erosion face appears after ruptured blister,
It does not recover for a long time, effective nursing can promote conditions of patients to restore to a certain extent.
Pemphigus is autoimmune disease, and in clinical treatment, glucocorticoid is choice drug, however, long-term use
Glucocorticoid is treated, glucocorticoid have immunosuppressive side effect, mechanism first is that inhibit macrophage gulp down
Bite and handle the process of antigen.
Wild marjoram is Labiatae origanum wild marjoram Origanum vulgare L., with all herbal medicine.Wild marjoram is thin to macrophage
Born of the same parents' phagocytic function has facilitation, can be improved body humoral immunity and cellular immunity and in vitro have antibacterial and antimycotic work
With.
Fibroin albumen is derived from silk, is a kind of natural polymer albumen, includes 18 kinds of amino acid, including a variety of human bodies must
The amino acid of palpus, and biocompatibility well has no toxic side effect, and has certain protection and trophism to human skin.Shell
Glycan, which has, improves the effects of being immunized, adjusting body environment, in field of functional materials, is used for medical neck frequently as membrane material
Domain.Cellulose is also existing one kind high-molecular compound in nature, because it is with good biocompatibility, degradability
And it is increasingly taken seriously.
If researching and developing a kind of composite membrane using wild marjoram cellulose, fibroin albumen chitosan as raw material, then not only easy to use, nothing
Toxic side effect, and have the function of enhancing immune, antibacterial, nutrition skin, it can be used for skin lesions of pemphigus vulgaris position
Nursing, accelerates the rehabilitation course of Pemphigus Vulgaris.
Summary of the invention
In order to solve foregoing problems, the present invention provides a kind of for the compound of skin lesions of pemphigus vulgaris position nursing
Film, which is prepared by wild marjoram cellulose, fibroin albumen, chitosan, not only easy to use, is had no toxic side effect, and
Have the function of enhancing immune, antibacterial, nutrition skin, can be used for the nursing at skin lesions of pemphigus vulgaris position.
A kind of composite membrane for the nursing of skin lesions of pemphigus vulgaris position, is achieved through the following technical solutions:
(1) wild marjoram cellulose is prepared；
(2) wild marjoram cellulose and silk fibroin composite membrane are prepared；
(3) chitosan function solution is prepared；
(4) preparation wild marjoram cellulose/fibroin albumen/chitosan complex film is used for skin lesions of pemphigus vulgaris to get one kind
The composite membrane of position nursing.
Further, the wild marjoram cellulose smashes it through 100 mesh the preparation method comprises the following steps: take the dry herb of Chinese medicine wild marjoram
Sieve is added deionized water refluxing extraction, Aqueous extracts is collected by filtration and dry filter residue；Wild marjoram filter residue is taken to be mixed with stupid with dehydrated alcohol
Close solution degreasing；It is dried in vacuo after degreasing, it is mechanical under the conditions of 60 DEG C that 2%-5%KOH solution then is added according to solid-to-liquid ratio 1: 5
2-4h is stirred, KOH solution is filtered out and is washed with deionized water to neutrality；30%H is added according still further to solid-to-liquid ratio 1: 52O245 DEG C of solution
Water-bath 2h, filters out H2O2Solution is simultaneously cleaned filter residue with deionized water, is pulverized after 60 DEG C of drying spare.
Further, during the deionized water refluxing extraction wild marjoram, deionized water and wild marjoram are according to solid-to-liquid ratio 1:
(10-20) is added, reflux extracting time 2-5h, merges extracting solution after refluxing extraction 2-3 times, and it is corresponding to be concentrated into every 1mL
Primary dose 0.10g.
Further, the method for the wild marjoram filter residue degreasing are as follows: stupid and ethanol solution is mixed according to 3: 1 ratio
It closes, is added with wild marjoram filter residue according to the ratio of solid-to-liquid ratio 1: 5, refluxing extraction 3h.
Further, the wild marjoram cellulose and silk fibroin composite membrane are fitted the preparation method comprises the following steps: weighing wild marjoram cellulose
Amount is added into [BMIM] Cl ionic liquid, and 200-400W ultrasonic wave added dissolves 15-30min, and fibroin albumen is then added, after
Continuous ultrasound 15-30min.Ionic liquid dissolved with wild marjoram cellulose and fibroin albumen is poured into tape casting in mold, then
It is placed in dehydrated alcohol and impregnates, taken off after wild marjoram cellulose and silk fibroin composite membrane solidification, be placed in deionized water and stand
Ionic liquid is precipitated in 2-5h, 3-6 times repeatedly, then takes out composite membrane, natural air drying, and wild marjoram fiber, plain fibroin egg is just made
White composite membrane.
Further, the wild marjoram cellulose, in silk fibroin composite membrane preparation process, wild marjoram cellulose, [BMIM]
The adding proportion of Cl ionic liquid and fibroin albumen is (1-3) g: 100mL: (0.5-2) g.
Further, the chitosan function solution preparation method are as follows: wild marjoram extracting solution is taken, suspended matter is filtered to remove,
Chitosan is added according to 100mL: 1.5g ratio, the dissolution of 200W power ultrasonic wave added is configured to chitosan function solution.
Further, wild marjoram cellulose/fibroin albumen/chitosan complex film is the preparation method comprises the following steps: by wild marjoram fibre
Dimension element, silk fibroin composite membrane are impregnated into 5min in chitosan function solution, are taken out natural air drying to get one kind and are used for vulgaris
The composite membrane of pemphigus cutaneous lesion nursing.
Beneficial effects of the present invention:
A kind of composite membrane for the nursing of skin lesions of pemphigus vulgaris position of the present invention, the composite membrane is with wild marjoram fibre
Dimension element, fibroin albumen and chitosan are made of raw material, and biocompatibility is good, and having enhances immune, antibacterial, nutrition skin function
Can, it can be used for the nursing at skin lesions of pemphigus vulgaris position.
Detailed description of the invention
Fig. 1 is the production technological process of composite membrane of the present invention.
Specific embodiment
Preparation method of the invention is completely described below in conjunction with specific embodiments.
Embodiment 1
The present invention provides a kind of composite membranes for the nursing of skin lesions of pemphigus vulgaris position, and the composite membrane is by wild marjoram fibre
Dimension element, fibroin albumen, chitosan are prepared, and preparation step is as follows:
(1) wild marjoram cellulose is prepared；
(2) wild marjoram cellulose and silk fibroin composite membrane are prepared；
(3) chitosan function solution is prepared；
(4) preparation wild marjoram cellulose/fibroin albumen/chitosan complex film is used for skin lesions of pemphigus vulgaris to get one kind
The composite membrane of position nursing.
The wild marjoram cellulose is prepared by the following method to obtain: taking the dry herb of Chinese medicine wild marjoram, smashes it through 100 mesh
Sieve is added deionized water refluxing extraction 5h according to solid-to-liquid ratio 1: 12, filters, extracting solution is merged, and be concentrated after refluxing extraction 3 times
Primary dose 0.10g is corresponded to every 1mL；Filter residue is dried；It is mixed stupid again with dehydrated alcohol according to 3: 1 ratio, with wild marjoram
Filter residue is added according to the ratio of solid-to-liquid ratio 1: 5, refluxing extraction 3h degreasing；Degreasing substrate vacuum drying, then according to solid-to-liquid ratio 1: 5
2%KOH solution mechanical stirring 4h under the conditions of 60 DEG C is added, filters out KOH solution and is washed with deionized water to neutrality；According still further to
30%H is added in solid-to-liquid ratio 1: 52O245 DEG C of water-bath 2h of solution, filter out H2O2Solution is simultaneously cleaned filter residue with deionized water, 60 DEG C
It pulverizes after drying spare.
The wild marjoram cellulose and silk fibroin composite membrane are prepared by the following method to obtain: wild marjoram cellulose 1g is weighed,
It is added into [BMIM] Cl ionic liquid 100mL, 200W ultrasonic wave added dissolves 30min, and fibroin albumen 2g is then added, continues to surpass
Sound 30min.Ionic liquid dissolved with wild marjoram cellulose and fibroin albumen is poured into tape casting in mold, is subsequently placed in anhydrous
Impregnated in ethyl alcohol, after wild marjoram cellulose and silk fibroin composite membrane solidification after take off, be placed in deionized water stand 2h be precipitated from
Sub- liquid 6 times repeatedly, then takes out composite membrane, natural air drying, and wild marjoram cellulose silk fibroin composite membrane is just made.
The chitosan function solution manufacturing method are as follows: wild marjoram extracting solution is taken, suspended matter is filtered to remove, according to 100mL:
Chitosan is added in the ratio of 1.5g, and the dissolution of 200W power ultrasonic wave added is configured to chitosan function solution.
Wild marjoram cellulose/fibroin albumen/the chitosan complex film is the preparation method comprises the following steps: by wild marjoram cellulose, fibroin
Protein composite film is impregnated into 5min in chitosan function solution, is taken out natural air drying to get one kind and is used for pemphigus vulgaris skin
Damage the composite membrane of position nursing.
Embodiment 2
The present invention provides a kind of composite membranes for the nursing of skin lesions of pemphigus vulgaris position, and the composite membrane is by wild marjoram fibre
Dimension element, fibroin albumen, chitosan are prepared, and preparation step is as follows:
(1) wild marjoram cellulose is prepared；
(2) wild marjoram cellulose and silk fibroin composite membrane are prepared；
(3) chitosan function solution is prepared；
(4) preparation wild marjoram cellulose/fibroin albumen/chitosan complex film is used for skin lesions of pemphigus vulgaris to get one kind
The composite membrane of position nursing.
The wild marjoram cellulose is prepared by the following method to obtain: taking the dry herb of Chinese medicine wild marjoram, smashes it through 100 mesh
Sieve is added deionized water refluxing extraction 3h according to solid-to-liquid ratio 1: 15, filters, extracting solution is merged, and be concentrated after refluxing extraction 3 times
Primary dose 0.10g is corresponded to every 1mL；Filter residue is dried；It is mixed stupid again with dehydrated alcohol according to 3: 1 ratio, with wild marjoram
Filter residue is added according to the ratio of solid-to-liquid ratio 1: 5, refluxing extraction 3h degreasing；Degreasing substrate vacuum drying, then according to solid-to-liquid ratio 1: 5
3%KOH solution mechanical stirring 3h under the conditions of 60 DEG C is added, filters out KOH solution and is washed with deionized water to neutrality；According still further to
45 DEG C of water-bath 2h of 30%H2O2 solution are added in solid-to-liquid ratio 1: 5, filter out H2O2 solution and are cleaned filter residue with deionized water, 60 DEG C
It pulverizes after drying spare.
The wild marjoram cellulose and silk fibroin composite membrane are prepared by the following method to obtain: wild marjoram cellulose 2g is weighed,
It is added into [BMIM] Cl ionic liquid 100mL, 300W ultrasonic wave added dissolves 20min, and fibroin albumen 1.5g is then added, and continues
Ultrasonic 25min.Ionic liquid dissolved with wild marjoram cellulose and fibroin albumen is poured into tape casting in mold, is subsequently placed in nothing
It is impregnated in water-ethanol, is taken off after wild marjoram cellulose and silk fibroin composite membrane solidification, be placed in deionized water and stand 3h precipitation
Ionic liquid 4 times repeatedly, then takes out composite membrane, natural air drying, and wild marjoram cellulose silk fibroin composite membrane is just made.
The chitosan function solution manufacturing method are as follows: wild marjoram extracting solution is taken, suspended matter is filtered to remove, according to 100mL:
Chitosan is added in the ratio of 1.5g, and the dissolution of 200W power ultrasonic wave added is configured to chitosan function solution.
Wild marjoram cellulose/fibroin albumen/the chitosan complex film is the preparation method comprises the following steps: by wild marjoram cellulose, fibroin
Protein composite film is impregnated into 5min in chitosan function solution, is taken out natural air drying to get one kind and is used for pemphigus vulgaris skin
Damage the composite membrane of position nursing.
Embodiment 3
The present invention provides a kind of composite membranes for the nursing of skin lesions of pemphigus vulgaris position, and the composite membrane is by wild marjoram fibre
Dimension element, fibroin albumen, chitosan are prepared, and preparation step is as follows:
(1) wild marjoram cellulose is prepared；
(2) wild marjoram cellulose and silk fibroin composite membrane are prepared；
(3) chitosan function solution is prepared；
(4) preparation wild marjoram cellulose/fibroin albumen/chitosan complex film is used for skin lesions of pemphigus vulgaris to get one kind
The composite membrane of position nursing.
The wild marjoram cellulose is prepared by the following method to obtain: taking the dry herb of Chinese medicine wild marjoram, smashes it through 100 mesh
Sieve is added deionized water refluxing extraction 2h according to solid-to-liquid ratio 1: 18, filters, extracting solution is merged, and be concentrated after refluxing extraction 2 times
Primary dose 0.10g is corresponded to every 1mL；Filter residue is dried；It is mixed stupid again with dehydrated alcohol according to 3: 1 ratio, with wild marjoram
Filter residue is added according to the ratio of solid-to-liquid ratio 1: 5, refluxing extraction 3h degreasing；Degreasing substrate vacuum drying, then according to solid-to-liquid ratio 1: 5
5%KOH solution mechanical stirring 2h under the conditions of 60 DEG C is added, filters out KOH solution and is washed with deionized water to neutrality；According still further to
30%H is added in solid-to-liquid ratio 1: 52O245 DEG C of water-bath 2h of solution, filter out H2O2Solution is simultaneously cleaned filter residue with deionized water, 60 DEG C
It pulverizes after drying spare.
The wild marjoram cellulose and silk fibroin composite membrane are prepared by the following method to obtain: wild marjoram cellulose 3g is weighed,
It is added into [BMIM] Cl ionic liquid 100mL, 400W ultrasonic wave added dissolves 15min, and fibroin albumen 1g is then added, continues to surpass
Sound 15min.Ionic liquid dissolved with wild marjoram cellulose and fibroin albumen is poured into tape casting in mold, is subsequently placed in anhydrous
Impregnated in ethyl alcohol, after wild marjoram cellulose and silk fibroin composite membrane solidification after take off, be placed in deionized water stand 5h be precipitated from
Sub- liquid 3 times repeatedly, then takes out composite membrane, natural air drying, and wild marjoram cellulose silk fibroin composite membrane is just made.
The chitosan function solution manufacturing method are as follows: wild marjoram extracting solution is taken, suspended matter is filtered to remove, according to 100mL:
Chitosan is added in the ratio of 1.5g, and the dissolution of 200W power ultrasonic wave added is configured to chitosan function solution.
Wild marjoram cellulose/fibroin albumen/the chitosan complex film is the preparation method comprises the following steps: by wild marjoram cellulose, fibroin
Protein composite film is impregnated into 5min in chitosan function solution, is taken out natural air drying to get one kind and is used for pemphigus vulgaris skin
Damage the composite membrane of position nursing.
The above, the only preference in all embodiments of the invention, but protection scope of the present invention is not limited to
This, it is any according to the technique and scheme of the present invention and its design be subject to equivalent substitution or change, should all cover in protection of the invention
In range.
Claims (9)
1. a kind of composite membrane for the nursing of skin lesions of pemphigus vulgaris position, which is characterized in that the composite membrane is by wild marjoram fiber
Element, fibroin albumen, chitosan are made, and preparation step includes:
(1) wild marjoram cellulose is prepared；
(2) wild marjoram cellulose and silk fibroin composite membrane are prepared；
(3) chitosan function solution is prepared；
(4) preparation wild marjoram cellulose/fibroin albumen/chitosan complex film is used for skin lesions of pemphigus vulgaris position to get one kind
The composite membrane of nursing.
2. compound membrane preparation method according to claim 1, which is characterized in that the preparation method of the wild marjoram cellulose
Are as follows: it takes Chinese medicine wild marjoram to dry herb, smashes it through 100 meshes, deionized water refluxing extraction is added, extracting solution is collected by filtration and incites somebody to action
Filter residue drying；Take wild marjoram filter residue with stupid and dehydrated alcohol mixed solution degreasing；It is dried in vacuo after degreasing, then according to solid-to-liquid ratio 1:
5 are added 2%-5%KOH solution mechanical stirring 2-4h under the conditions of 60 DEG C, filter out KOH solution and are washed with deionized water into
Property；30%H is added according still further to solid-to-liquid ratio 1: 52O245 DEG C of water-bath 2h of solution, filter out H2O2Solution and with deionized water by filter residue
It cleans, pulverizes after 60 DEG C of drying spare.
3. compound membrane preparation method according to claim 2, which is characterized in that the deionized water refluxing extraction wild marjoram
In the process, deionized water and wild marjoram are according to solid-to-liquid ratio 1: (10-20) addition, reflux extracting time 2-5h, refluxing extraction 2-3 times
Extracting solution is merged afterwards, and is concentrated into every 1mL and corresponds to primary dose 0.10g.
4. compound membrane preparation method according to claim 2, which is characterized in that the method for the wild marjoram filter residue degreasing
Are as follows: it mixes, is added with wild marjoram filter residue according to the ratio of solid-to-liquid ratio 1: 5, reflux according to 3: 1 ratio with ethanol solution by stupid
Extract 3h.
5. compound membrane preparation method according to claim 1, which is characterized in that the wild marjoram cellulose and fibroin albumen
The 200-400W ultrasonic wave added into [BMIM] Cl ionic liquid is added the preparation method comprises the following steps: to weigh wild marjoram cellulose appropriate in composite membrane
15-30min is dissolved, fibroin albumen is then added, continues ultrasound 15-30min.It will be dissolved with wild marjoram cellulose and fibroin albumen
Ionic liquid pours into tape casting in mold, is subsequently placed in dehydrated alcohol and impregnates, compound to wild marjoram cellulose and fibroin albumen
It is taken off after film solidification, is placed in deionized water and stands 2-5h precipitation ionic liquid, 3-6 times repeatedly, then take out composite membrane, from
It so air-dries, wild marjoram cellulose, silk fibroin composite membrane is just made.
6. compound membrane preparation method according to claim 5, which is characterized in that the wild marjoram cellulose, fibroin albumen
In composite membrane preparation process, the adding proportion of wild marjoram cellulose, [BMIM] Cl ionic liquid and fibroin albumen is (1-3) g:
100mL∶(0.5-2)g。
7. compound membrane preparation method according to claim 1, which is characterized in that the chitosan function solution preparation side
Method are as follows: take wild marjoram extracting solution, be filtered to remove suspended matter, chitosan, 200W power ultrasound is added according to 100mL: 1.5g ratio
Auxiliary dissolution is configured to chitosan function solution.
8. compound membrane preparation method according to claim 1, which is characterized in that the wild marjoram cellulose/fibroin albumen/
Chitosan complex film the preparation method comprises the following steps: impregnating wild marjoram cellulose, silk fibroin composite membrane into chitosan function solution
5min takes out natural air drying to get a kind of composite membrane for the nursing of skin lesions of pemphigus vulgaris position.
9. a kind of composite membrane prepared such as any one of claim 1-8.
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