CN109394966A - A kind of mulberry leaf compound and preparation method thereof - Google Patents
A kind of mulberry leaf compound and preparation method thereof Download PDFInfo
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- CN109394966A CN109394966A CN201811323618.6A CN201811323618A CN109394966A CN 109394966 A CN109394966 A CN 109394966A CN 201811323618 A CN201811323618 A CN 201811323618A CN 109394966 A CN109394966 A CN 109394966A
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- mulberry leaf
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Abstract
Mulberry leaf compound is prepared using classification ultrafiltration, ultramicro grinding method for combined use the invention discloses a kind of, it belongs to traditional Chinese medicine and field of food industry.A kind of mulberry leaf compound, it is mainly prepared by following raw materials by the parts by weight: 10-20 parts of mulberry leaf, 2-10 parts of hawthorn, 2-7 parts of radix polygonati officinalis, 2-7 parts of fructus lycii.It is raw material that the present invention, which selects the Chinese medicine of integration of drinking and medicinal herbs, has the function of the wide diaphragm of invigorating the spleen, helping digestion promoting the circulation of qi, nourishing liver and kidney, Yin-nourishing and body fluid promoting, can be prepared into drink, without any side effects.Preparation method of the invention, it prepares mulberry leaf complex composition using classification ultrafiltration, ultramicro grinding joint technology, it destroys raw material cell wall using classification ultrafiltration, ultramicro grinding and effective component in cell is precipitated, effective component structure will not be damaged simultaneously, mulberry leaf compound combination ultra-micro powder obtained is set to play curative effect quickly, effectively conditioning " three high ".
Description
Technical field
The present invention relates to a kind of using classification ultrafiltration, pulverizes the mulberry leaf compound of method for combined use preparation, it belongs to
In traditional Chinese medicine and field of food industry.
Background technique
" three high " refers to hypertension, hyperglycemia and hyperlipemia, is " rich people's disease " that modern society is derived from, can be with
Individualism, it is also possible to interrelated.Such as diabetes patient is easy to suffer from hypertension or hyperlipemia simultaneously, and hyperlipidemia
It is the principal element of artery sclerosis formation and development, arteriosclerotic's blood vessel elasticity difference is aggravated blood pressure and increased.So there is this
Any one of three kinds of illness, later period all easily form " three high diseases ".
Doctor trained in Western medicine is in the treatment of " three high diseases ", mostly drug combination.The drug of clinical treatment hypertension mostly uses Yi Napu
Benefit, sodium nitroprussiate, reserpine, chlorthalidone etc., fluctuation of blood pressure is larger in pressure reduction；Clinically adjusting blood lipid Western medicine drug is mainly
Statins, fibrate, niacin class drug, bile acid chelating medicine, easily rebound, drug effect are difficult persistently, want during lipid-loweringing
It inspects periodically liver function and whether various adverse reaction indications occurs, adjust medication；Clinical treatment diabetes mostly use sulphur
Ureas, biguanides, meglitinide, alpha-glucosidase inhibitor, thiazolidinediones etc., hypoglycemic process blood glucose fluctuation is larger, needs forever
Long medication.
" three high diseases " belong in Chinese medicine scope caused by yin-Yang imbalance, mostly because of environmental change, injury due to diet, feelings will
Caused by imbalance etc., pathogenesis is mainly the dysfunction of the spleen in transport, and yang-energy virtual loss, heart-fire hyperactivity, phlegm is turbid to be pented up, qi and blood stagnation and cost
Disease.
Chemical composition of Chinese materia medica is complicated, and traditional is used as medicine mode based on decocting, and decoction process is along with mass efficient ingredient
It is lost, and medicinal ingredient cannot be precipitated completely, the process time is long, and energy consumption is high.With modern medicine and pharmacology development in science and technology, with tradition
Experience extractive technique have been unable to meet the needs of prophylactic treatment, and effective component of chinese medicine is typically distributed on cell wall and is wrapped in
Cell interior, the recovery rate of Yao Tigao target product and make full use of resource, need to take effective ways destroy its cell wall and
Effective component in cell is precipitated, while too big destruction will not be caused to effective component structure, it is seen that effective component of chinese medicine
It extracts more and more important.
Summary of the invention
In view of the deficienciess of the prior art, an object of the present invention is to provide a kind of mulberry leaf compound for improving " three high "
Composition, selecting the Chinese medicine of integration of drinking and medicinal herbs is raw material, has the work of the wide diaphragm of invigorating the spleen, helping digestion promoting the circulation of qi, nourishing liver and kidney, Yin-nourishing and body fluid promoting
With, it can be prepared into drink, it is without any side effects.
It is a further object of the present invention to provide the preparation methods of mulberry leaf compound, it is using classification ultrafiltration, Ultramicro-powder
Broken joint technology prepares mulberry leaf complex composition, it destroys raw material cell wall using classification ultrafiltration, ultramicro grinding and makes in cell
Effective component is precipitated, while will not damage to effective component structure, enables mulberry leaf compound combination ultra-micro powder obtained very
It is fast to play curative effect, effectively improve " three high ".
To achieve the goals above, the invention provides the following technical scheme:
A kind of mulberry leaf compound, it is mainly prepared by following raw materials by the parts by weight: mulberry leaf 10-20
Part, 2-10 parts of hawthorn, 2-7 parts of radix polygonati officinalis, 2-7 parts of fructus lycii.
A kind of above-mentioned mulberry leaf compound, it is mainly prepared by following raw materials by the parts by weight: mulberry
15 parts of leaf, 6 parts of hawthorn, 4.5 parts of radix polygonati officinalis, 4.5 parts of fructus lycii.
The preparation method of above-mentioned mulberry leaf compound, preparation methods steps are as follows:
(1) mulberry leaf for meeting the parts by weight, hawthorn, radix polygonati officinalis, fructus lycii are weighed, through co-grinding to 20-60 mesh, is obtained slightly
Medicinal powder is spare；
(2) thick medicinal powder is placed in medicine pot, first time amount of water is 18-22 times of thick medicinal powder weight, and it is small to impregnate 0.5-1.5
When, the cellulase of 0.2-0.4 parts by weight is added, uses citric acid-sodium citrate tune pH value for 4.0-6.0, in 40 DEG C -60 DEG C
It digests constant temperature in pot to digest 2-4 hours, after enzymatic hydrolysis, is warming up to 93-97 DEG C of enzyme deactivation；
(3) after enzyme deactivation, enzymatic treatment system is boiled, slightly boiled decoction 1-3 hours, is filtered, collect filtrate；Filter residue adds for the second time
Water is 16-20 times of thick medicinal powder weight, and after boiling, filtrate is collected in slightly boiled decoction 0.5-1.5 hours, filtration；Filter residue third time
Amount of water is 13-17 times of thick medicinal powder weight, and after boiling, filtrate is collected in slightly boiled decoction 0.5-1.5 hours, filtration；
(4) the resulting filtrate three times of step (3) is merged, 4000-6000r/min is centrifuged 10-20min, collects supernatant；
(5) it is super by molecular weight 300K, 100K, 10K doughnut that retention is respectively adopted in the supernatant for collecting step (4)
Filter column carries out ultrafiltration, collects ultrafiltrate；
(6) by step (5) resulting ultrafiltrate, under the conditions of 40-50 DEG C, relative molecular mass 300- is retained using film
600Da carries out nanofiltration concentration, and the volume of the concentrated liquid is the 1/4-3/4 of stoste volume, obtains concentrate；
(7) it to the maltodextrin of 0.1-0.5 times of concentrate weight of addition in step (6) resulting concentrate, stirs evenly
After be spray-dried, spray drying condition is as follows: 110-130 DEG C of inlet air temperature, 80-100 DEG C of leaving air temp, atomizing pressure
750-850Pa, hydrojet speed 8-12%, obtains dried powder；
(8) dried powder obtained by step (7) is put into micronizer to pulverize, passes through high energy nanometer impact grinding
The intracorporal mill Jie impacting 5-15min of tank makes the average grain diameter of Ultramicro-powder reach 900-1500nm to get the combination of mulberry leaf compound
Ultra-micro powder.
40 mesh are crushed to after the mixing of above-mentioned steps (1) raw material.
First time amount of water is 20 times of thick medicinal powder weight in above-mentioned steps (2), and soaking time is preferably 1 hour, fiber
Plain enzyme additional amount is preferably 0.3 parts by weight, and pH value is preferably 5.0, and hydrolysis temperature is preferably 50 DEG C, and enzymolysis time is preferably 3 small
When, preferably 95 DEG C of enzyme-removal temperature.
First time decocting time is preferably 2 hours after enzymatic hydrolysis in above-mentioned steps (3), and second of amount of water is preferably thick medicinal powder
18 times of weight, decocting time are preferably 1 hour, and third time amount of water is preferably 15 times of thick medicinal powder weight, and decocting time is excellent
It is selected as 1 hour.
Centrifugation rate is preferably 5000r/min in above-mentioned steps (4), and centrifugation time is preferably 15min.
Nanofiltration temperature is preferably 45 DEG C in above-mentioned steps (6), and retaining molecular weight is preferably 450Da.
The additive amount of maltodextrin is preferably 0.3 times of concentrate weight in above-mentioned steps (7)；Spray drying EAT
It is preferred that 120 DEG C, preferably 90 DEG C of leaving air temp, the preferred 800Pa of atomizing pressure, hydrojet speed preferably 10%.
The preferred 10min of impacting in above-mentioned steps (8), the average grain diameter of Ultramicro-powder are 1200nm.
Due to the adoption of the above technical scheme, so that compared with prior art, the present invention have following features and the utility model has the advantages that
It is as follows the effect of Chinese medicine material used in the present invention: mulberry leaf: Ji Yuan: for the leaf of moraceae plants mulberry；Nature and flavor: cold；Bitter,
It is sweet；Channel tropism: enter lung, Liver Channel；Major function: dispelling wind and heat from the body, clearing away the lungheat and moisturizing, calming the liver and improving eyesight；For anemopyretic cold, lung-heat type cough,
Dizziness headache, red eyes and dim-sighted.Hawthorn: Ji Yuan: the dry mature fruit of rosaceous plant large-fruited Chinese hawthorn or hawthorn.Nature and flavor: acid is sweet, micro-
Temperature；Channel tropism: enter spleen, stomach, Liver Channel；Major function: digestion gathered food, scattered blood stasis, expelling tenia；For meat accumulation, abdominal mass, phlegm retention, feeling of fullness gulped down
Acid, dysentery, intestines wind, pain in the loins, postpartum abdominal pain in hernias, lochiorrhagia, pediatric milk food stagnation.Radix polygonati officinalis: Ji Yuan: for monocotyledon
The fresh or dry rhizome of grass family reed；Nature and flavor: it is sweet, it trembles with fear；Channel tropism: enter lung channel, stomach meridian.Major function: clearing heat and promoting fluid, removing
Tired, preventing or arresting vomiting, diuresis；For pyreticosis and polydipsia, gastropyretic vomiting, cough with lung heat, lung carbuncle pyemesis, puckery pain of heat gonorrhea.Fructus lycii: Ji Yuan: Solanaceae
The dry mature fruit of plant lycium barbarum；Nature and flavor: sweet and neutral；Channel tropism: enter liver and kidney channel；Major function: nourishing the liver, nourshing kidney, moistening lung；
For consumption consumptive loss, soreness of waist and knee joint, dizziness and tinnitus, impotence and seminal emission, Heat Diabetes, blood deficiency chlorosis, blurred vision.
The present invention improves Chinese medicine used in the mulberry leaf compound of " three high ", selects medicine in national integration of drinking and medicinal herbs catalogue
Material is raw material, based on TCM theory, adheres to the theory of TCM preventiveed treatment of disease, and is that principle carries out prescription by monarch, strictly
The proportion for controlling each component, has effects that the wide diaphragm of invigorating the spleen, helping digestion promoting the circulation of qi, nourishing liver and kidney, Yin-nourishing and body fluid promoting, carries out daily tune to body
Reason and maintenance prevent state of an illness generation and development, achieve the purpose that treating both manifestation and root cause of disease.The present invention is by above-mentioned mulberry leaf, hawthorn, radix polygonati officinalis, Chinese holly
Qi is combined by the dosage, so that each raw material generates synergistic function, index ingredient mulberry leaf polysaccharide contains in compound
Measuring more single mulberry leaf can be promoted twice.Mulberry leaf report golden gas and then get both the clear respectful of the world through frost again, clear west it is dry, rush down east it
It is real, obtain the taste of Central-South fiery soil.It is equipped with hawthorn helping digestion promoting the circulation of qi, the yin of fructus lycii nourishing liver kidney, reed root clearing heat and promoting fluid, group is wide with invigorating the spleen
Diaphragm enhances kidney hydraulic quantity, to restrict internal heat, declines internal heat heating power, blood viscousness, blood lipid is avoided to increase.Meanwhile internal heat
After decline, cell interior moisture retains, and the aqueous vapor reduced in organ-tissue forms moisture.
Present invention application classification ultrafiltration, ultramicro grinding joint technology prepare mulberry leaf compound, and traditional Chinese medicine is extracted
Technique is combined with modern biotechnology technique, retains the extracting mode of traditional water decoction decoction, will using enzymatic hydrolysis pre-treating technology
Protein, cellulose, pectic substance on cell wall etc. hinder the main barrier degradation of effective component dissolution intracellular, to make cell
Wall is loose, ruptures, and reduces diffusion of active ingredient resistance intracellular, improves recovery rate.The present invention is adopted for raw material sources in composition
It uses cellulase as enzymolysis agent, can effectively decompose ingredient between cell wall and cell wall.It is found by Experimental comparison, hydrolysis temperature
Most important to mulberry leaf complex composition Degree of Enzymatic Hydrolysis with enzymolysis time, hydrolysis temperature increases, and enzymatic activity rises, reactant energy
Increase, accelerates reaction speed.After temperature is more than the optimal temperature of cellulase, zymoprotein can be caused to be denaturalized, enzyme activity
Weaken significantly, until vigor is completely lost, reaction speed decline, so that the decomposition to cell is influenced, so the present invention digests temperature
Degree selection is between 40 DEG C -60 DEG C.After screening, best enzymolysis time is 3 hours, such as extends enzymolysis time, raw cell again
It destroys seriously, activity decline, content is declined.When gradually increasing with cellulase dosage, the molten of mulberry leaf polysaccharide can be increased
Out, when enzyme dosage reaches 0.2-0.4 parts by weight, the position of enzyme-to-substrate reaches saturation state, and mulberry leaf polysaccharide yield reaches
Balance.Enzyme dosage is such as continued growing, cell wall damage is complete, changes the leaching environments of mulberry leaf polysaccharide, yield is caused to reduce.?
It is found in research process, enzymatic hydrolysis acidity has certain influence, the ability only in certain pH value range to index ingredient yield
Play catalytic action.When pH value is between 4.0-6.0, yield reaches peak value, and pH value increases again, and yield declines instead.Pass through
After digesting parameter optimization, mulberry leaf polysaccharide index content promotes three times compared with traditional extraction technique.It goes to remove water using biological hyperfiltration technique
The macromoleculars inert substance such as starch, protein in extracting solution, while loss of effective components is reduced, recycle the filter of nanofiltration biotechnology
Water removal is crossed, it is active constituent-enriched, while can avoid thermophilic digestion feed liquid in concentrated extracting solution, effective component retention rate is improved,
Reduce heat energy loss.Obtained Ultramicro-powder is after water dissolves, liquid clear, no residue deposited phenomenon.The present invention exists simultaneously
In preparation process, it is not introduced into organic solvent and chemical reagent, does not destroy medicinal material internal component structure, protects character of medicinal material, more preferably
Ground plays health care curative effect.
The mulberry leaf compound that the present invention improves " three high " can be used for preparing alternative tea.
Present invention application classification ultrafiltration, ultramicro grinding joint technology prepare mulberry leaf compound, super through what is be prepared
Micro mist can be used for preparing mulberry leaf compound combined beverage series of products.
Present invention application classification ultrafiltration, ultramicro grinding joint technology prepare mulberry leaf compound in terms of improving " three high "
Effect it is preferable, alleviate that symptom effect is obvious, and " hypertension ", " hyperlipidemia " and " hyperglycemia " disease and its bring are alleviated in prevention
Other health problems.
It is all medicine food that present invention application classification ultrafiltration, ultramicro grinding joint technology, which prepare mulberry leaf compound selected materials,
Homologous Chinese medicine, raw material are easy to get, without any side effects, can reduce the body liver kidney burden and financial burden of user.
Present invention application is classified ultrafiltration, ultramicro grinding joint technology prepares mulberry leaf compound, after Oral Administration in Rats, mulberry leaf
It absorbs and is distributed more rapid after flavones ingredient, after medication after 4 hours, reach Cmax the 2nd time, illustrate it in enteron aisle
Residence time is longer.Folium Mori alkaloid constituents absorbed into serum, blood concentration can be reached in gastrointestinal tract at 30 minutes or so quickly
To peak value, can be quickly distributed after prompting it to enter rat body circulation.
Preparation method of the invention, medicinal ingredient is precipitated completely in it, and low energy consumption, it is using classification ultrafiltration, ultramicro grinding
It destroys raw material cell wall and effective component in cell is precipitated, while effective component structure will not be damaged, made obtained
Mulberry leaf compound combination ultra-micro powder can play curative effect quickly.
Specific embodiment
Below with reference to embodiment, the present invention is described further.Following embodiment is only several specific realities of the invention
Example is applied, but the design concept of the present invention is not limited to this, it is all to be made a non-material change to the present invention using this design,
It should belong to the behavior for invading the scope of the present invention.
Method in following embodiments is unless otherwise instructed conventional method.
Percentage composition in following embodiments is unless otherwise instructed mass percentage.
Number in following embodiments is weight fraction.
Embodiment 1
A kind of mulberry leaf compound, it is to be prepared by following raw materials by the parts by weight: 10 parts of mulberry leaf, hawthorn
10 parts, 7 parts of radix polygonati officinalis, 7 parts of fructus lycii.
The preparation method of mulberry leaf compound, steps are as follows:
(1) 10 parts by weight mulberry leaf, 10 parts by weight hawthorn, 7 parts by weight radix polygonati officinalis, 7 parts by weight fructus lyciis are weighed, extremely through co-grinding
It is spare to obtain thick medicinal powder for 20 mesh；
(2) thick medicinal powder is placed in medicine pot, first time amount of water is 18 times of thick medicinal powder weight, is impregnated 0.5 hour, is added
The cellulase of 0.2 parts by weight uses citric acid-sodium citrate tune pH value as 4.0, and constant temperature enzymatic hydrolysis 2 is small in 40 DEG C of enzymatic hydrolysis pots
When, after enzymatic hydrolysis, it is warming up to 93 DEG C of enzyme deactivations；
(3) after enzyme deactivation, enzymatic treatment system is boiled, slightly boiled to decoct 1 hour, filtrate is collected in filtration；Filter residue adds water for the second time
Amount is 16 times of thick medicinal powder weight, slightly boiled to decoct 0.5 hour after boiling, and filtrate is collected in filtration；Filter residue third time amount of water is
13 times of thick medicinal powder weight, slightly boiled to decoct 0.5 hour after boiling, filtrate is collected in filtration；
(4) the resulting filtrate three times of step (3) is merged, 4000r/min is centrifuged 10min, collects supernatant；
(5) it is super by molecular weight 300K, 100K, 10K doughnut that retention is respectively adopted in the supernatant for collecting step (4)
Filter column carries out ultrafiltration, collects ultrafiltrate；
(6) it by step (5) resulting ultrafiltrate, under the conditions of 40 DEG C, is carried out using film retention relative molecular mass 300Da
Nanofiltration concentration, the volume of the concentrated liquid are the 1/4 of stoste volume, obtain concentrate；
(7) it to the maltodextrin of 0.1 times of concentrate weight of addition in step (6) resulting concentrate, stirs evenly laggard
Row spray drying；Spray drying condition is as follows: 110 DEG C of inlet air temperature, 80 DEG C of leaving air temp, atomizing pressure 750Pa, and hydrojet speed
8%；
(8) it takes dried powder obtained by step (7) to be put into micronizer to be pulverized, passes through high energy nanometer impact grinding
The intracorporal mill Jie impacting 5min of tank makes the average grain diameter of Ultramicro-powder reach 900nm and combines Ultramicro-powder to get mulberry leaf compound
End.
Embodiment 2
A kind of mulberry leaf compound, it is to be prepared by following raw materials by the parts by weight: 12 parts of mulberry leaf, hawthorn
8 parts, 5.5 parts of radix polygonati officinalis, 5.5 parts of fructus lycii.
The preparation method of mulberry leaf compound, steps are as follows:
(1) 12 parts by weight mulberry leaf, 8 parts by weight hawthorn, 5.5 parts by weight radix polygonati officinalis, 5.5 parts by weight fructus lyciis are weighed, through mixed powder
30 mesh are broken to, it is spare to obtain thick medicinal powder；
(2) thick medicinal powder is placed in medicine pot, first time amount of water is 19 times of thick medicinal powder weight, is impregnated 0.8 hour, is added
The cellulase of 0.25 parts by weight, uses citric acid-sodium citrate tune pH value as 4.5, the constant temperature enzymatic hydrolysis 2.5 in 45 DEG C of enzymatic hydrolysis pots
Hour, after enzymatic hydrolysis, it is warming up to 94 DEG C of enzyme deactivations；
(3) after enzyme deactivation, enzymatic treatment system is boiled, slightly boiled to decoct 1.5 hours, filtrate is collected in filtration；Filter residue adds for the second time
Water is 17 times of thick medicinal powder weight, slightly boiled to decoct 0.8 hour after boiling, and filtrate is collected in filtration；Filter residue third time amount of water
It is 14 times of thick medicinal powder weight, after boiling, slightly boiled to decoct 0.8 hour, filtrate is collected in filtration；
(4) the resulting filtrate three times of step (3) is merged into 4500r/min and is centrifuged 12min, collect supernatant；
(5) it is super by molecular weight 300K, 100K, 10K doughnut that retention is respectively adopted in the supernatant for collecting step (4)
Filter column carries out ultrafiltration, collects ultrafiltrate；
(6) it by step (5) resulting ultrafiltrate, under the conditions of 43 DEG C, is carried out using film retention relative molecular mass 400Da
Nanofiltration concentration, the volume of the concentrated liquid are the 1/4 of stoste volume, obtain concentrate；
(7) it to the maltodextrin of 0.2 times of concentrate weight of addition in step (6) resulting concentrate, stirs evenly laggard
Row spray drying；Spray drying condition is as follows: 115 DEG C of inlet air temperature, 85 DEG C of leaving air temp, atomizing pressure 780Pa, and hydrojet speed
9%.
(8) it takes dried powder obtained by step (7) to be put into micronizer to be pulverized, passes through high energy nanometer impact grinding
The intracorporal mill Jie impacting 8min of tank makes the average grain diameter of Ultramicro-powder reach 1000nm and combines Ultramicro-powder to get mulberry leaf compound
End.
Embodiment 3
A kind of mulberry leaf compound, it is to be prepared by following raw materials by the parts by weight: 15 parts of mulberry leaf, hawthorn
6 parts, 4.5 parts of radix polygonati officinalis, 4.5 parts of fructus lycii.
The preparation method of mulberry leaf compound, steps are as follows:
(1) 15 parts by weight mulberry leaf, 6 parts by weight hawthorn, 4.5 parts by weight radix polygonati officinalis, 4.5 parts by weight fructus lyciis are weighed, through mixed powder
40 mesh are broken to, it is spare to obtain thick medicinal powder；
(2) thick medicinal powder is placed in medicine pot, first time amount of water is 20 times of thick medicinal powder weight, is impregnated 1 hour, is added
The cellulase of 0.3 parts by weight uses citric acid-sodium citrate tune pH value as 5.0, and constant temperature enzymatic hydrolysis 3 is small in 50 DEG C of enzymatic hydrolysis pots
When, after enzymatic hydrolysis, it is warming up to 95 DEG C of enzyme deactivations；
(3) after enzyme deactivation, enzymatic treatment system is boiled, slightly boiled to decoct 2 hours, filtrate is collected in filtration；Filter residue adds water for the second time
Amount is 18 times of thick medicinal powder weight, slightly boiled to decoct 1 hour after boiling, and filtrate is collected in filtration；Filter residue third time amount of water is thick
15 times of medicinal powder weight, slightly boiled to decoct 1 hour after boiling, filtrate is collected in filtration；
(4) the resulting filtrate three times of step (3) is merged, 5000r/min is centrifuged 15min, collects supernatant；
(5) it is super by molecular weight 300K, 100K, 10K doughnut that retention is respectively adopted in the supernatant for collecting step (4)
Filter column carries out ultrafiltration, collects ultrafiltrate；
(6) it by step (5) resulting ultrafiltrate, under the conditions of 45 DEG C, is carried out using film retention relative molecular mass 450Da
Nanofiltration concentration, the volume of the concentrated liquid are the 1/2 of stoste volume, obtain concentrate；
(7) it to the maltodextrin of 0.3 times of concentrate weight of addition in step (6) resulting concentrate, stirs evenly laggard
Row spray drying；Spray drying condition is as follows: 120 DEG C of inlet air temperature, 90 DEG C of leaving air temp, atomizing pressure 800Pa, and hydrojet speed
10%.
(8) it takes dried powder obtained by step (7) to be put into micronizer to be pulverized, passes through high energy nanometer impact grinding
The intracorporal mill Jie impacting 10min of tank makes the average grain diameter of Ultramicro-powder reach 1200nm and combines Ultramicro-powder to get mulberry leaf compound
End.
Embodiment 4
A kind of mulberry leaf compound, it is to be prepared by following raw materials by the parts by weight: 17 parts of mulberry leaf, hawthorn
4 parts, 3.5 parts of radix polygonati officinalis, 3.5 parts of fructus lycii.
The preparation method of mulberry leaf compound, steps are as follows:
(1) 17 parts by weight mulberry leaf, 4 parts by weight hawthorn, 3.5 parts by weight radix polygonati officinalis, 3.5 parts by weight fructus lyciis are weighed, through mixed powder
50 mesh are broken to, it is spare to obtain thick medicinal powder；
(2) thick medicinal powder is placed in medicine pot, first time amount of water is 21 times of thick medicinal powder weight, is impregnated 1.2 hours, is added
The cellulase of 0.35 parts by weight, uses citric acid-sodium citrate tune pH value as 5.5, the constant temperature enzymatic hydrolysis 3.5 in 55 DEG C of enzymatic hydrolysis pots
Hour, after enzymatic hydrolysis, it is warming up to 96 DEG C of enzyme deactivations；
(3) after enzyme deactivation, enzymatic treatment system is boiled, slightly boiled to decoct 2.5 hours, filtrate is collected in filtration；Filter residue adds for the second time
Water is 19 times of thick medicinal powder weight, slightly boiled to decoct 1.2 hours after boiling, and filtrate is collected in filtration；Filter residue third time amount of water
It is 16 times of thick medicinal powder weight, after boiling, slightly boiled to decoct 1.2 hours, filtrate is collected in filtration；
(4) the resulting filtrate three times of step (3) is merged, 5500r/min is centrifuged 17min, collects supernatant；
(5) it is super by molecular weight 300K, 100K, 10K doughnut that retention is respectively adopted in the supernatant for collecting step (4)
Filter column carries out ultrafiltration, collects ultrafiltrate；
(6) it by step (5) resulting ultrafiltrate, under the conditions of 47 DEG C, is carried out using film retention relative molecular mass 500Da
Nanofiltration concentration, the volume of the concentrated liquid are the 3/4 of stoste volume, obtain concentrate；
(7) it to the maltodextrin of 0.4 times of concentrate weight of addition in step (6) resulting concentrate, stirs evenly laggard
Row spray drying；Spray drying condition is as follows: 125 DEG C of inlet air temperature, 95 DEG C of leaving air temp, atomizing pressure 830Pa, and hydrojet speed
11%.
(8) it takes dried powder obtained by step (7) to be put into micronizer to be pulverized, passes through high energy nanometer impact grinding
The intracorporal mill Jie impacting 12min of tank makes the average grain diameter of Ultramicro-powder reach 1300nm and combines Ultramicro-powder to get mulberry leaf compound
End.
Embodiment 5
A kind of mulberry leaf compound, it is to be prepared by following raw materials by the parts by weight: 20 parts of mulberry leaf, hawthorn
2 parts, 2 parts of radix polygonati officinalis, 2 parts of fructus lycii.
The preparation method of mulberry leaf compound, steps are as follows:
(1) 20 parts by weight mulberry leaf, 2 parts by weight hawthorn, 2 parts by weight radix polygonati officinalis, 2 parts by weight fructus lyciis are weighed, extremely through co-grinding
It is spare to obtain thick medicinal powder for 60 mesh；
(2) thick medicinal powder is placed in medicine pot, first time amount of water is 22 times of thick medicinal powder weight, is impregnated 1.5 hours, is added
The cellulase of 0.4 parts by weight uses citric acid-sodium citrate tune pH value as 6.0, and constant temperature enzymatic hydrolysis 4 is small in 60 DEG C of enzymatic hydrolysis pots
When, after enzymatic hydrolysis, it is warming up to 97 DEG C of enzyme deactivations；
(3) after enzyme deactivation, enzymatic treatment system is boiled, slightly boiled to decoct 3 hours, filtrate is collected in filtration；Filter residue adds water for the second time
Amount is 20 times of thick medicinal powder weight, slightly boiled to decoct 1.5 hours after boiling, and filtrate is collected in filtration；Filter residue third time amount of water is
17 times of thick medicinal powder weight, slightly boiled to decoct 1.5 hours after boiling, filtrate is collected in filtration；
(4) the resulting filtrate three times of step (3) is merged, 6000r/min is centrifuged 20min, collects supernatant；
(5) it is super by molecular weight 300K, 100K, 10K doughnut that retention is respectively adopted in the supernatant for collecting step (4)
Filter column carries out ultrafiltration, collects ultrafiltrate；
(6) it by step (5) resulting ultrafiltrate, under the conditions of 50 DEG C, is carried out using film retention relative molecular mass 600Da
Nanofiltration concentration, the volume of the concentrated liquid are the 3/4 of stoste volume, obtain concentrate；
(7) it to the maltodextrin of 0.5 times of concentrate weight of addition in step (6) resulting concentrate, stirs evenly laggard
Row spray drying；Spray drying condition is as follows: 130 DEG C of inlet air temperature, 100 DEG C of leaving air temp, atomizing pressure 850Pa, and hydrojet speed
Degree 12%.
(8) it takes dried powder obtained by step (7) to be put into micronizer to be pulverized, passes through high energy nanometer impact grinding
The intracorporal mill Jie impacting 15min of tank makes the average grain diameter of Ultramicro-powder reach 1500nm and combines Ultramicro-powder to get mulberry leaf compound
End.
Thick medicinal powder obtained by step (1) can be applied to prepare mulberry leaf compound alternative tea in embodiment 1,2,3,4,5.
The combination of mulberry leaf compound obtained by step (8) ultra-micro powder can be used for mulberry leaf compound combination drink in embodiment 1,2,3,4,5
Expect series of products.
Test example 1
The detection and content point of the mulberry leaf compound combination ultra-micro powder ingredient of 1,2,3,4,5 method of embodiment of the present invention preparation
Analysis method and result are as follows:
(1) prepared by reference substance solution: it is appropriate that precision weighs glucose control product, and with distilled water constant volume, 0.5mg/mL is made
Reference substance solution.
(2) drafting of standard curve respectively precision pipette glucose control product solution 0.5,1.0,1.5,2.0,2.5mL in
In 10mL volumetric flask, it is diluted with water to scale, respectively takes 1.0mL in 10mL colorimetric cylinder, is separately added into 5% phenol solution 1.0mL,
It shakes up, the 5.0mL concentrated sulfuric acid is added, shakes up immediately, boiling water bath heats 10min, is cooled to room temperature with ice water.Made with distilled water with method
Blank reference surveys absorbance value (A) at 485nm.Using its concentration as abscissa, absorbance value is ordinate, and it is bent to draw standard
Line.
(3) test solution preparation weighs the mulberry leaf compound combination ultra micro of 1,2,3,4,5 method of embodiment of the present invention preparation
Each 1.0g of powder adds 80% ethyl alcohol 100mL, 40-50 DEG C, ultrasonic 30min is volatilized to the ethyl alcohol in residue in stuffed conical flask
Afterwards, add water 100mL, 40-50 DEG C, each 30min, ultrasonic extraction 3 times, filtering merges 3 filtrates, after being evaporated, is transferred to 50mL
Measuring bottle in.By standard curve method " taking 1.0mL in 10mL colorimetric cylinder ..., to be cooled to room temperature with ice water ", as examination
Product solution.
Mulberry leaf simple preparation precision weighs mulberry leaf, extracts by 3 extracting method of embodiment, by test solution method system
For to get mulberry leaf single drug solns.
Traditional water decoction agent group sample preparation weighs 15 parts of mulberry leaf, 6 parts of hawthorn, radix polygonati officinalis 4.5 in 3 step of embodiment (1) ratio
Part, 4.5 parts of fructus lycii, carry out being crushed to 40 mesh after mixing.First time amount of water is 20 times of medicinal material amount, is impregnated 1 hour, slightly boiled to decoct
It boils 2 hours, filters, collect filtrate；Second of amount of water of filter residue is 18 times of medicinal material amount, slightly boiled to decoct 1 hour after boiling, filter
It crosses, collects filtrate；Filter residue third time amount of water is 15 times of medicinal material amount, slightly boiled to decoct 1 hour after boiling, and filter is collected in filtration
Liquid.Filtrate, moisture volatilize three times for merging.Precision weighs 1.0g medicinal extract, prepares by test solution method to get traditional water decoction agent
Group sample solution.
(4) experimental result
1 reference substance concentration of table and absorbance linear relationship table
| Concentration of glucose/mg/mL | 0.025 | 0.05 | 0.075 | 0.10 | 0.125 |
| Light absorption value | 0.197 | 0.345 | 0.539 | 0.712 | 0.862 |
Calibration curve equation is Y=6.788X+0.0219, coefficient R=0.9991
The measurement comparison of 2 mulberry leaf polysaccharide component content of table
| Grouping | Mulberry leaf polysaccharide content % |
| Example 1 group | 34.82 |
| 2 groups of embodiment | 37.74 |
| 3 groups of embodiment | 40.03 |
| 4 groups of embodiment | 38.64 |
| 5 groups of embodiment | 36.32 |
| Mulberry leaf simple group | 12.38 |
| Traditional water decoction agent group | 9.77 |
The comparison of 3 nutrient component determining of table
Test example 2
The mulberry leaf compound combination ultra-micro powder of 1,2,3,4,5 method of embodiment of the present invention preparation is dynamic for spontaneous hypertension
Object model test.
Animal packet and administration: being randomly divided into 10 groups by basic blood pressure for 80 spontaneous hypertensive rat (SHR) rats,
That is blank control group (drinking water), 1,2,3,4,5 group of embodiment, mulberry leaf simple group and traditional water decoction agent group, every group 10.Respectively
Embodiment group, mulberry leaf simple group and traditional water decoction agent group dosage are 0.21g/kg, and administration capacity is 10mL/kg, each group
Corresponding drug or drinking water are given in daily stomach-filling.It weighs in weekly 1 time, and adjusts dosage according to weight.Experiment periods
Between each group raise with normal diet, and free water, administration time is 6 weeks.
(1) blood pressure determination: measuring rat tail artery systolic pressure, diastolic pressure using BP-6 animal non-invasive blood pressure test macro,
1 week measuring blood pressure 1 time during administration, every animal duplicate measurements 3 times, be averaged.
(2) experimental result
4 mulberry leaf compound of table compares the variation of each group rat different time systolic pressure
Note: compared with blank control group,*P < 0.05,**P<0.01
Compared with mulberry leaf simple group, #P < 0.05, ##P < 0.01
Compared with traditional water decoction agent group,△P < 0.05,△△P<0.01
5 mulberry leaf compound of table compares the variation of each group rat different time diastolic pressure
Note: compared with blank control group,*P < 0.05,**P<0.01
Compared with mulberry leaf simple group, #P < 0.05, ##P < 0.01
Compared with traditional water decoction agent group,△P < 0.05,△△P<0.01
By table 4-5 it is found that during to drug 6 weeks, mulberry leaf compound combines the systolic pressure and diastole that can reduce SHR rat
Pressure, wherein 3 groups of effects of embodiment are more apparent (P < 0.01)；And mulberry leaf simple group and traditional water decoction agent group control hypertension auxiliary
Treatment effect is slow.
Test example 3
The mulberry leaf compound combination ultra-micro powder of 1,2,3,4,5 method of embodiment of the present invention preparation is dynamic for induction property hyperlipidemia
Object model test.
(1) animal packet and administration: choosing 90 4 week old Kunming mouses of male, after adaptable fed 5d, is randomly divided into
9 groups, every group 10, respectively normal group, control group high in fat, 1,2,3,4,5 group of embodiment, mulberry leaf simple group and traditional water decoction
Agent group, each test group dosage are 0.3g/kg.Normal group feeding basal diet, high in fat group, each embodiment group, mulberry leaf single
Medicine group and traditional water decoction agent group feed high lipid food, and after stomach-filling 28d, mouse fasting 12h or more drinks water as usual, measurement.
(2) testing index: rat fasting 12h after the last administration does not cut off the water supply, weighs its scale of construction.Disconnected neck is put to death, and blood is taken, low
Temperature centrifugation (3000r/min) 10min, takes serum to measure serum total cholesterol (TC), triglycerides using kit enzymic colorimetric
(TG), high-density lipoprotein (HDLC), low-density lipoprotein (LDLC) calculate atherosclerosis index (AI).
(3) experimental result:
Influence (n=10) of the 6 mulberry leaf compound of table to mouse weight (g)
| Group | Original body mass | First week | Second week | Third week | 4th week |
| Blank group | 27.80±1.34 | 29.45±0.54 | 31.63±0.32 | 34.02±1.04 | 36.48±0.60 |
| High in fat group | 27.05±1.53 | 31.73±1.66 | 35.52±1.05* | 38.77±0.39* | 40.11±2.67* |
| Example 1 group | 27.41±0.89 | 30.76±1.24 | 31.12±1.45▲#△ | 32.39±1.06▲#△ | 34.02±1.83▲#△ |
| 2 groups of embodiment | 27.55±1.43 | 30.32±0.37 | 32.12±1.27▲#△ | 33.29±0.72▲#△ | 34.61±1.70▲#△ |
| 3 groups of embodiment | 27.43±0.79 | 28.87±1.02 | 30.48±1.59▲#△ | 31.57±1.17▲#△ | 32.63±0.70▲#△ |
| 4 groups of embodiment | 27.78±0.62 | 29.43±0.78 | 30.69±0.84▲#△ | 32.54±1.25▲#△ | 33.73±1.61▲#△ |
| 5 groups of embodiment | 27.64±1.21 | 30.95±0.57 | 31.88±0.98▲#△ | 33.79±0.89▲#△ | 35.15±1.01▲#△ |
| Mulberry leaf simple group | 27.38±0.99 | 31.99±1.92 | 34.39±2.01 | 36.49±1.34 | 38.11±2.47 |
| Traditional water decoction agent group | 27.49±1.37 | 32.16±1.53 | 34.47±1.14 | 36.13±1.42 | 38.71±1.54 |
Note: compared to the blank group,*P<0.05；
Compared with high in fat group,▲P<0.05
Compared with mulberry leaf simple group,#P<0.05
Compared with traditional water decoction agent group,△P<0.05
Influence (n=10) of the 7 mulberry leaf compound of table to lipid of mice level and antiatherosclerosis index
| Group | TC(mmoL/L) | TG(mmoL/L) | LDL-C(mmoL/L) | HDL-C(mmoL/L) | AI |
| Blank group | 3.02±0.26 | 1.25±0.08 | 1.80±0.11 | 1.96±0.12 | 1.84±0.05 |
| High in fat group | 5.11±0.32* | 1.60±0.11* | 3.39±0.08* | 1.70±0.13* | 0.05±0.03* |
| Example 1 group | 4.07±0.33▲#△ | 1.44±0.11▲#△ | 2.22±0.16▲#△ | 2.12±0.28▲#△ | 1.74±0.13▲▲#△ |
| 2 groups of embodiment | 4.12±0.25▲#△ | 1.32±0.08▲▲##△△ | 2.18±0.14▲#△ | 2.23±0.06▲#△ | 1.71±0.08▲▲#△ |
| 3 groups of embodiment | 3.22±0.38▲▲##△△ | 1.26±0.13▲▲##△△ | 2.05±0.09▲▲##△△ | 2.45±0.17▲#△ | 1.90±0.04▲▲##△△ |
| 4 groups of embodiment | 3.84±0.19▲#△ | 1.35±0.05▲#△ | 2.33±0.21▲#△ | 2.41±0.08▲#△ | 1.86±0.11▲▲##△△ |
| 5 groups of embodiment | 3.91±0.27▲#△ | 1.34±0.09▲#△ | 2.11±0.18▲▲##△△ | 2.38±0.07▲#△ | 1.78±0.09▲▲#△ |
| Mulberry leaf simple group | 4.76±0.29 | 1.52±0.10 | 2.78±0.91 | 1.86±0.34 | 1.18±0.09▲ |
| Traditional water decoction agent group | 4.81±0.33 | 1.52±0.16 | 2.83±0.14 | 1.88±0.21 | 1.29±0.06▲ |
Note: compared to the blank group,*P<0.05
Compared with high in fat group,▲P<0.05
Compared with mulberry leaf simple group,#P<0.05
Compared with traditional water decoction agent group,△P<0.05
From table 6-7 it is found that from the 2nd week, high in fat group of mouse weight dramatically increases (P < 0.05) compared with other groups.Implement
Example each group, mulberry leaf simple group and traditional water decoction agent group can accomplish to slow down weight gain.For hyperlipidemia model group, mulberry
Leaf compound, which combines each embodiment group, can reduce mice serum TC content, TG content, LDL-C content, improve HDL-C level,
3 groups of reduction TC contents of middle embodiment, TG content, LDL-C content significant effect (P < 0.05), and improve HDL-C it is horizontal significant (P <
0.05).From resist athero- atherogenic index (AI) result it is found that mulberry leaf compound combine each embodiment group can significantly improve it is high in fat
Rat AI index (P < 0.05), and effect is better than mulberry leaf simple group and traditional water decoction agent group.Although mulberry leaf simple and prescription
Traditional water decoction agent also has certain change blood lipids index, but function and effect are unobvious.
Test example 4
The mulberry leaf compound combination ultra-micro powder of 1,2,3,4,5 method of embodiment of the present invention preparation is used for diabetes animal model
Test.
(1) experimental method:
1.1 choose mouse fasting 24 hours, give alloxan (45mg/kg iv), 5 hours measurement tail blood of fasting after 7 days
Blood glucose.Tail blood blood glucose value hyperglycemia model animal 80 within the scope of 10-25mmol/L, are divided into model control group by blood glucose value,
1,2,3,4,5 group of embodiment, mulberry leaf simple group and traditional water decoction agent group, each group dosage are 0.78g/
(kg·d)。
Healthy mice 10 is separately taken to be only used as blank control group, each group mouse blood sugar value before and after comparative experiments.
After 1.2 above-mentioned hypoglycemic experiments, each test group gives the mulberry leaf compound combination that different preparation methods obtain, each group
Dosage is 0.78g/ (kgd), and blank control group and model control group are given same volume distilled water, given after 15min through mouth
2.0g/kg glucose solution is given, 0,0.5,1,2 hour after tail hematometry glucose blood glucose value is taken, observation each group is giving grape
The variation of each time point Area under the curve of blood glucose after sugar juice.
(2) experimental result:
8 mulberry leaf compound of table combines the influence (n=10) to blood glucose in diabetic mice
Note: compared with blank control group,#P < 0.05,##P<0.01；
Compared with model control group,*P < 0.05,**P<0.01；
Compared with after modeling,△P < 0.05,△△P<0.01；
Compared with mulberry leaf simple group,▲P < 0.05,▲▲P<0.01；
Compared with traditional water decoction agent group,▼P < 0.05,▼▼P<0.01。
9 mulberry leaf compound of table combines the influence (n=10) to diabetic mice sugar tolerance
Note: compared with blank control group,#P < 0.05,##P<0.01
Compared with model control group,*P < 0.05,**P<0.01
Compared with mulberry leaf simple group,△P < 0.05,△△P<0.01
Compared with traditional water decoction agent group,▲P < 0.05,▲▲P<0.01
By table 8-9 it is found that mulberry leaf compound, which combines, can significantly reduce blood glucose in diabetic mice level, and with administration time
Extension, each embodiment group reduces that blood glucose level is more significant, and wherein 3 groups of reduction hypoglycemic effects of embodiment are better than other each groups, and
Mulberry leaf simple group and traditional water decoction agent group effect be not significant.Mulberry leaf compound, which combines each embodiment group, can reduce diabetic mice Portugal
Blood glucose value after grape glucose load reduces the area (AUC) under postprandial blood sugar time curved surface, improves diabetic mice sugar tolerance energy
Power is numerically better than other each groups for 3 groups of embodiment, and the effect of mulberry leaf compound combination raising diabetic mice sugar tolerance is excellent
In mulberry leaf simple group and traditional water decoction agent group.
Claims (10)
1. a kind of mulberry leaf compound, it is characterised in that it is mainly prepared by following raw materials by the parts by weight:
10-20 parts of mulberry leaf, 2-10 parts of hawthorn, 2-7 parts of radix polygonati officinalis, 2-7 parts of fructus lycii.
2. a kind of mulberry leaf compound according to claim 1, it is characterised in that it mainly presses institute by following raw materials
It states parts by weight to be prepared: 15 parts of mulberry leaf, 6 parts of hawthorn, 4.5 parts of radix polygonati officinalis, 4.5 parts of fructus lycii.
3. a kind of preparation method of mulberry leaf compound as claimed in claim 1 or 2, it is characterised in that preparation methods steps are such as
Under:
(1) mulberry leaf for meeting the parts by weight, hawthorn, radix polygonati officinalis, fructus lycii are weighed, through co-grinding to 20-60 mesh, obtains thick medicinal powder
It is spare；
(2) thick medicinal powder being placed in medicine pot, first time amount of water is 18-22 times of thick medicinal powder weight, it impregnates 0.5-1.5 hours,
The cellulase of 0.2-0.4 parts by weight is added, uses citric acid-sodium citrate tune pH value for 4.0-6.0, in 40 DEG C of -60 DEG C of enzymes
It solves constant temperature in pot to digest 2-4 hours, after enzymatic hydrolysis, is warming up to 93-97 DEG C of enzyme deactivation；
(3) after enzyme deactivation, enzymatic treatment system is boiled, slightly boiled decoction 1-3 hours, is filtered, collect filtrate；Second of amount of water of filter residue
It is 16-20 times of thick medicinal powder weight, after boiling, filtrate is collected in slightly boiled decoction 0.5-1.5 hours, filtration；Filter residue third time plus water
Amount is 13-17 times of thick medicinal powder weight, and after boiling, filtrate is collected in slightly boiled decoction 0.5-1.5 hours, filtration；
(4) the resulting filtrate three times of step (3) is merged, 4000-6000r/min is centrifuged 10-20min, collects supernatant；
(5) retention is respectively adopted by molecular weight 300K, 100K, 10K Hollow Fiber Ultrafiltration column in the supernatant for collecting step (4)
Ultrafiltration is carried out, ultrafiltrate is collected；
(6) by step (5) resulting ultrafiltrate, under the conditions of 40-50 DEG C, relative molecular mass 300-600Da is retained using film
Nanofiltration concentration is carried out, the volume of the concentrated liquid is the 1/4-3/4 of stoste volume, obtains concentrate；
(7) it to the maltodextrin of 0.1-0.5 times of concentrate weight of addition in step (6) resulting concentrate, stirs evenly laggard
Row spray drying, spray drying condition are as follows: 110-130 DEG C of inlet air temperature, 80-100 DEG C of leaving air temp, and atomizing pressure 750-
850Pa, hydrojet speed 8-12%, obtains dried powder；
(8) dried powder obtained by step (7) is put into micronizer to pulverize, passes through high energy nanometer impact grinding tank body
Interior mill Jie impacting 5-15min makes the average grain diameter of Ultramicro-powder reach 900-1500nm and combines ultra micro to get mulberry leaf compound
Powder.
4. the preparation method of mulberry leaf compound according to claim 3, which is characterized in that after the mixing of step (1) raw material
It is crushed to 40 mesh.
5. the preparation method of mulberry leaf compound according to claim 3, which is characterized in that add for the first time in step (2)
Water is 20 times of thick medicinal powder weight, and soaking time is 1 hour, and cellulase additional amount is 0.3 parts by weight, pH value 5.0, enzyme
Solving temperature is 50 DEG C, and enzymolysis time is 3 hours, and enzyme-removal temperature is 95 DEG C.
6. the preparation method of mulberry leaf compound according to claim 3, which is characterized in that in step (3) the after enzymatic hydrolysis
Decocting time is 2 hours, and second of amount of water is 18 times of thick medicinal powder weight, and decocting time is 1 hour, third time plus water
Amount is 15 times of thick medicinal powder weight, and decocting time is 1 hour.
7. the preparation method of mulberry leaf compound according to claim 3, which is characterized in that centrifugation rate in step (4)
For 5000r/min, centrifugation time 15min.
8. the preparation method of mulberry leaf compound according to claim 3, which is characterized in that nanofiltration temperature in step (6)
It is 45 DEG C, retaining molecular weight 450Da.
9. the preparation method of mulberry leaf compound according to claim 3, which is characterized in that maltodextrin in step (7)
Additive amount be 0.3 times of concentrate weight；Spray drying condition is as follows: 120 DEG C of inlet air temperature, 90 DEG C of leaving air temp, and atomization
Pressure 800Pa, hydrojet speed 10%.
10. the preparation method of mulberry leaf compound according to claim 3, which is characterized in that impacting in step (8)
For 10min, the average grain diameter of Ultramicro-powder is 1200nm.
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|---|---|---|---|---|
| CN1919027A (en) * | 2006-08-25 | 2007-02-28 | 华雪芳 | Healthy tea and its preparing method |
| CN101884739A (en) * | 2009-05-15 | 2010-11-17 | 天津太平洋制药有限公司 | Food therapy preparation for preventing and treating diabetes based on theories of preventive treatment of disease and medicine food homology |
| CN107149618A (en) * | 2017-05-15 | 2017-09-12 | 英山县桑和平面茧专业合作社 | A kind of vinegar mulberry leaf blood sugar reduction tea and preparation method thereof |
-
2018
- 2018-11-08 CN CN201811323618.6A patent/CN109394966A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1919027A (en) * | 2006-08-25 | 2007-02-28 | 华雪芳 | Healthy tea and its preparing method |
| CN101884739A (en) * | 2009-05-15 | 2010-11-17 | 天津太平洋制药有限公司 | Food therapy preparation for preventing and treating diabetes based on theories of preventive treatment of disease and medicine food homology |
| CN107149618A (en) * | 2017-05-15 | 2017-09-12 | 英山县桑和平面茧专业合作社 | A kind of vinegar mulberry leaf blood sugar reduction tea and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 孙培桐，等: "治疗高脂血症47例临床观察", 《上海中医药杂志》 * |
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