CN109393568B - Burley tobacco extract for heating non-combustible cigarettes and preparation method thereof - Google Patents
Burley tobacco extract for heating non-combustible cigarettes and preparation method thereof Download PDFInfo
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Classifications
-
- A24F47/008—
Landscapes
- Manufacture Of Tobacco Products (AREA)
Abstract
The invention provides a burley tobacco extract for heating non-burning cigarettes and application thereof, which adopts a burley tobacco rod and bagasse composite biomaterial as an immobilized carrier, an adsorption biological agent comprises a microorganism aroma-producing strain and enzyme, the burley tobacco powder is incubated, hydrolyzed and fermented, and then a continuous countercurrent ultrasonic solvent extraction technology is combined to prepare the burley tobacco extract which is compounded with propylene glycol, so that the burley tobacco extract has strong natural tobacco aroma, the burley tobacco characteristic smell is reduced, the irritation is reduced, the burley tobacco extract has natural composite aromas such as fresh fruit aroma, caramel smell, baking aroma and the like, the process conditions are mild, and the extraction rate and the production efficiency are improved; the white burley tobacco is applied to novel heating non-burning cigarette materials, is coordinated with tobacco fragrance and unified in style, increases the smoke concentration, improves the satisfaction, enriches the tobacco fragrance, obviously lightens the irritation smell of the white burley tobacco and improves the taste.
Description
Technical Field
The invention relates to the field of smokeless cigarettes, in particular to a burley tobacco extract for heating non-combustible cigarettes and a preparation method thereof.
Background
The additive for tobacco is an assistant for improving the physical and chemical properties of tobacco, and comprises spice essence, a humectant, a combustion improver, a mildew preventive and an adsorbent. Compared with the traditional combustion type cigarette, the heating temperature of the non-combustion type cigarette is generally 500 ℃ or less, usually 300 ℃, the action on aroma substances in the cigarette and the mechanism for generating aroma are different, the sensory effect difference with the cigarette smoking is often larger, and the flavoring is needed to make up for the deficiency of the cigarette aroma. The natural perfume meets the requirements of consumers on health better due to natural property, safety and natural compound aroma, but still has the defects of low extraction efficiency, more solvent residues, high impurity content, low purity, high cost caused by complex purification steps, obvious characteristic aroma, additional aroma for covering cigarette aroma and the like. Because most of the perfume materials are compounds with lower molecular weight, low boiling point and stronger volatility, the loss is serious in the high-temperature processing process, so that the phenomenon of nonuniform fragrance release is caused, and the fragrance is lost due to volatilization even in the storage period or the chemical property is unstable and is easy to be oxidized and deteriorated.
Burley tobacco and safflower tobacco seed. It originates from raw materials of hybrid cigarettes in Ohio, USA, and can also be used for cigar, pipe and chewing tobacco. The burley tobacco has thicker main veins, higher moisture content, thin and light leaves, loose and not rough tissue structure, strong elasticity, high filling value, good performance of absorbing spices, higher content of nicotine and total nitrogen than that of flue-cured tobacco, strong smoke strength, low sugar content, strong irritation, unobvious fragrance and insufficient fragrance, and has very strong absorption capacity, and in the general process, the burley tobacco absorbs more than 40 percent of sugar, the sun-cured tobacco absorbs 20 to 30 percent of the sugar, and the flue-cured tobacco only absorbs 10 to 20 percent. Burley tobacco has the functions of regulating fragrance and eating flavor, and is an important raw material of blended cigarettes; in the prior art, the burley tobacco is used in the flue-cured type cigarette products in a small amount according to the proportion of 2-4%.
The burley tobacco is used as a heating non-burning cigarette additive, volatile components of the burley tobacco are tobacco original substances, common tobacco extracts are dissolved and heated and extracted through a solvent, the extracts contain a certain amount of macromolecular compounds, and the burley tobacco extracts are particularly used for electronic cigarettes, electric heating novel cigarettes, fuel heating type tobacco products and the like, are difficult to atomize at a lower temperature to generate smoke, are difficult to evaporate out fragrant substances and more in impurities, increase the fragrance of cigarettes and have the defects of increasing irritation, miscellaneous gas, residual feeling and the like, and the burley tobacco extracts often have the characteristic smell of burley tobacco and are large in irritation.
At present, the patent 'preparation method of burley tobacco charging material base' (application No. 201510420420.X) is a tobacco natural amino acid and application thereof in burley tobacco, buffer solution is added into burley tobacco waste powder, 0.1% -0.3% of protease is added, the pH value of a reaction system is adjusted to 4.6-6.0, the reaction system is heated to 40-60 ℃ and reacted for 4-6 h, the same amount of protease is added, then the mixture and sugar are mixed and applied to burley tobacco leaves, and the organoleptic quality is improved through baking treatment; the patent 'a preparation method of sun-cured yellow tobacco extract for novel tobacco products' (application number 201610988163.4) is that sun-cured yellow tobacco is added with water and heated for reflux extraction, crude extract is filtered by gauze and a 0.8 micron filter membrane, then a compound amino acid or ammonia substance with the weight of 2-30% and a solvent with the weight of 0-20% of the weight of the sun-cured yellow tobacco water extract are added, the pH value is 2-11, the mixture is stirred, heated and reacted for 1-7 hours under normal pressure for natural cooling to obtain the sun-cured yellow tobacco Maillard reactant, and finally the sun-cured yellow tobacco Maillard reactant is placed in a filler rectification tower for rectification, and liquid substances with the density of 1.0479-1.1158 at the top of the tower are collected to be the sun-cured yellow tobacco extract, and can be directly added into novel tobaccos such as electronic cigarette oil, buccal tobacco, snuff, tobacco spray and the like; the patent of 'a lining material for improving the fragrance harmony of burley tobacco and a preparation method and application thereof' (application No. 201610962696.5) relates to a lining material for improving the fragrance harmony of burley tobacco, which comprises a plurality of components in percentage by mass: 5-15% of invert sugar, 5-10% of glacial acetic acid, 1-3% of glycerol, 1-2% of lining base flavor and 70-88% of hot water at 60 ℃, wherein the sum of the components is 100%. The invert sugar is prepared by adding 50 parts of water into 100 parts of sucrose to prepare a saturated solution, adding citric acid accounting for 3.0 percent of the weight of the sucrose, and reacting for 2 hours at 80-100 ℃. The burley tobacco flavor formula provided by the invention obviously weakens the characteristic aroma of the burley tobacco, enables the burley tobacco flavor to be coordinated with the flue-cured tobacco flavor, achieves the purpose of improving the burley tobacco flavor coordination, is stable in physicochemical indexes and strong in use pertinence, and can be conveniently used in the traditional cigarette feed liquid application process.
However, the biological agent is fixed by compounding the burley tobacco stem and the bagasse with the biological adsorption material, the burley tobacco leaves are incubated, hydrolyzed and fermented, and the continuous countercurrent ultrasonic extraction technology is combined to obtain the burley tobacco extract which is compounded with propylene glycol and applied to the heating non-combustible cigarettes, and no relevant report is found yet.
Disclosure of Invention
According to the defects, the burley tobacco extract for heating non-burning cigarettes and the preparation method thereof are adopted, the burley tobacco stem and bagasse composite biological material is used as an immobilized carrier, an adsorption biological agent comprises a microorganism aroma-producing strain and enzyme, the burley tobacco powder is incubated, hydrolyzed and fermented, and then the continuous countercurrent ultrasonic solvent extraction technology is combined to prepare the burley tobacco extract which is compounded with propylene glycol, so that the burley tobacco extract has the advantages of natural rich natural tobacco, reduced burley tobacco characteristic smell, reduced irritation, fresh fruit smell, caramel smell, baking smell and other natural composite aromas, mild process conditions and improved extraction rate and production efficiency; the white burley tobacco is applied to novel heating non-burning cigarette materials, is coordinated with tobacco fragrance and unified in style, increases the smoke concentration, improves the satisfaction, enriches the tobacco fragrance, obviously lightens the irritation smell of the white burley tobacco and improves the taste.
The invention is realized by the following technical scheme:
a method for preparing a burley tobacco extract for heating a non-combustible cigarette, comprising the steps of:
(2) pretreatment: crushing burley tobacco leaves into powder of 40-200 meshes by a crusher;
(2) activation of microbial strains: adding active freeze-dried powder of saccharomyces cerevisiae (Saccharomyces fibuligera) into 5-20 times of sterilized glucose solution with the mass concentration of 5-30%, adding succinic acid, adjusting the pH value to 1-6, and activating in a water bath kettle at 10-25 ℃ for 10-30 min to obtain a strain activation solution;
(3) preparing an immobilized carrier: cleaning and drying burley tobacco stems and bagasse, processing the tobacco stems into fragments with the length of about 1cm, mixing the tobacco stems with the bagasse, and drying after autoclaving to obtain a mixed biological material immobilized carrier;
(4) immobilization treatment of microbial strains: putting a certain amount of the mixed immobilized carrier sterilized and dried in the step (3) into a container, adding the cultured activating solution according to the mass ratio of the immobilized carrier to the microbial strain activating solution of 1: 1-10, and placing the mixture in a refrigerated cabinet at the temperature of 4 +/-0.5 ℃ for fixing for 12-36 hours to obtain an immobilized strain-carrier;
(5) enzyme immobilization treatment: dissolving asparaginase into an aqueous solution, wherein the mass ratio of the asparaginase to water is 0.01-0.05%, adding sodium carbonate to adjust the pH value to 7-10, stirring and uniformly mixing, adding the mixed biomaterial immobilized carrier in the step (3), and placing the mixed biomaterial immobilized carrier in a refrigerated cabinet at the temperature of 4 +/-0.5 ℃ for immobilization for 12-36 hours to obtain an immobilized enzyme-carrier;
(6) two-step fermentation of burley tobacco: uniformly mixing the burley tobacco powder obtained in the step (1) with water of which the mass is 5-15 times that of the burley tobacco powder, adding the immobilized strain-carrier obtained in the step (4), stirring at a constant speed at the temperature of 35-55 ℃, adding succinic acid to adjust the pH value to 1.0-6.0, and fermenting for 1-3 days; adding ammonia water, adjusting the pH value to 7-9, adding the immobilized enzyme-carrier obtained in the step (5), stirring uniformly at the temperature of 20-40 ℃, fermenting for 1-3 d, filtering burley tobacco powder residues, and keeping burley tobacco fermentation liquor A;
(7) continuous countercurrent ultrasonic extraction: feeding the burley tobacco residue obtained by filtering in the step (6) into a leaching cabin at the speed of 0.1-1.0 kg/10min, starting a main machine and an ultrasonic generator, adjusting the temperature to 40-60 ℃, preparing a propylene glycol aqueous solution with the mass ratio concentration of 50-80%, adding sodium carbonate, uniformly stirring, adjusting the pH value to 7-9, adjusting the mass ratio of the material to the liquid ratio to be 1: 2-6, operating for 0.2-1 h from the lower end of the leaching cabin, adjusting a liquid outlet valve to uniformly discharge liquid, controlling the adding flow of a solvent, keeping the liquid level of the leaching cabin balanced, feeding magnesium hydroxide and compound amino acid in a certain ratio when continuous countercurrent ultrasonic extraction reaches 30-60 min, stopping the production process when the continuous countercurrent ultrasonic extraction reaches 90-150 min, discharging slag, and obtaining a leaching solution B;
(8) and (4) combining the fermentation liquor A in the step (6) and the leaching liquor B in the step (7) to obtain the burley tobacco extract.
Further, the saccharomyces cerevisiae fibuligera in the step (2) is food grade, the activity is 10-30 u/mg, and the glucose and the succinic acid are food grade.
Further, the mass ratio of the burley tobacco stems to the bagasse in the step (3) is 1-10: 1, and the mass ratio of the mixed biomaterial immobilized carrier to the burley tobacco powder is 1: 3-10.
Further, the sodium carbonate in the step (5) is food grade, and the rotating speed of uniform stirring is 20-60 rpm.
Further, the magnesium hydroxide used in the step (7) is analytically pure and accounts for 0.1-1% of the mass of the burley tobacco, the compound amino acids are leucine, isoleucine and cysteine and are in a food grade and account for 0.5-1.5% of the mass of the burley tobacco, and the mass ratio of the leucine, the isoleucine, the cysteine and the food grade is 1: 0.5-2.
The burley tobacco extract is flavored according to the mass ratio of 0.001-0.07 percent of the burley tobacco extract to the tobacco shreds in the formula of the heated non-burning cigarettes, so that the cigarette is full in tobacco flavor, high in concentration, moderate in strength, high in satisfaction, and low in irritation smell of the burley tobacco, and the burley tobacco extract also has fresh apple aroma, bouquet, cheese roasting aroma and barbecue aroma.
The invention also provides application of the burley tobacco extract prepared by the method in novel cigarette paper for heating non-combustible cigarettes, the burley tobacco extract is coated on the cigarette paper according to 0.01-0.7% of the mass of the cigarette paper, the tobacco aroma of the novel cigarettes is increased, the satisfaction is improved, the aroma is enriched, the irritating smell of the burley tobacco is obviously reduced, and the burley tobacco extract has fresh apple aroma, wine aroma, roasted cheese aroma and roasted meat aroma and is improved in taste.
The invention also provides an application of the burley tobacco extract prepared by the method in a novel water-based adhesive for heating non-combustible cigarettes, wherein the burley tobacco extract is added into the water-based adhesive for cigarettes according to the mass ratio of 0.01-0.1%, is uniformly stirred, is applied according to normal process conditions in a wrapping procedure, and has the advantages of increasing smoke concentration, enriching tobacco aroma, obviously reducing burley tobacco irritation smell and having fresh apple aroma, wine aroma, roasted cheese aroma and roasted meat aroma when a novel cigarette product is heated.
The invention has the advantages that:
(1) the burley tobacco stems and the bagasse are used as a composite biological material carrier for adsorption immobilization of biological agents, wherein the burley tobacco stems are tobacco original substances, the cellulose contained in the burley tobacco stems is strong in adsorption effect, the waste tobacco stems are also used as resources, the bagasse left after sugar pressing has about 50% of fibers, the length of the fibers is about 0.65-2.17 mm, the width of the fibers is 21-28 mu m, the adsorption capacity is strong, after the burley tobacco stems and the bagasse are mixed according to a certain proportion, the efficiency of adsorbing and immobilizing microbial strains and enzymes under the synergistic effect is high, the enzymes are not easy to desorb, and the fermentation liquor is easy to filter and separate at the later stage, so that the purification cost is reduced, and the efficiency is improved;
(2) the burley tobacco leaf powder is subjected to fractional adsorption and fermentation by saccharomyces cerevisiae and asparaginase, so that the flavor is enhanced, and the thorn is reduced. The burley tobacco powder is dissolved in water, and in the process of incubation hydrolysis fermentation under an acidic condition with a complex enzyme system of Saccharomycosis fibulia adsorbed on burley tobacco stems and bagasse, the active strain has the characteristics of producing amylase, beta-glucosidase, acid protease and the like, can efficiently degrade macromolecular substances such as cellulose, starch, cellobiose, cane sugar and the like in the cell walls and cells of the burley tobacco powder, converts the macromolecular substances into glucose, further converts the macromolecular substances into aromatic substances and ethanol with ester fragrance as main parts, increases fresh bouquet, converts non-volatile glucoside into volatile aromatic substances, produces the aroma, produces the ester, removes miscellaneous odor, destroys the cell walls, releases more active chemical substances in the cells, improves the yield and increases the natural complexity of the aroma; during the second fermentation step, the asparaginase has high enzyme activity under alkaline conditions, converts asparagine in burley tobacco into aspartic acid, reduces burley tobacco characteristic irritation smell generated by asparagine Maillard reactants generated by Maillard reaction when burley tobacco is heated, and converts the burley tobacco characteristic irritation smell into pyrazine flue-cured tobacco smell generated by aspartic acid Maillard reactants. Under the synergistic and strong combined action of the microbial strains and the enzymes, the non-volatile glucoside is better converted into a volatile aroma component, bracket components such as cellulose, cellobiose and the like in cell walls are more effectively decomposed, intracellular active components and aroma substances are more released, and degraded polysaccharide substances are volatile aroma micromolecule components, and part of glucose can be converted into ethanol and released into fermentation liquor to form natural and rich fresh fruit aroma, wine aroma and milk aroma of the fermentation liquor, and the fermentation liquor can be separated from an immobilized carrier with the microbial strains through simple filtration, so that the steps are simple;
(3) and extracting burley tobacco fermentation material residues by combining a continuous countercurrent ultrasonic solvent, wherein the process conditions are mild, the production time is shortened, the production efficiency is improved, and the cost is reduced, magnesium hydroxide is added in the production process to further adsorb impurities which influence the taste absorption, such as tannin components, remained in the extracting solution, compound amino acid is added, and the compound amino acid, reducing sugar and other free amino acid in the burley tobacco extracting solution are subjected to a Maillard intermediate aroma enhancement reaction under the low-temperature heating condition of 40-60 ℃ in a countercurrent manner to generate an aldehyde aroma enhancement substance, so that the aroma is further enhanced and reduced.
Generally, impurity macromolecular components including protein, cellulose, polysaccharide, starch and the like are decomposed and converted into fragrant substances, impurity foreign taste is reduced, irritation is reduced, natural complexity of fragrance types is increased, fragrance quality is improved, burley tobacco extract has strong natural fragrance of natural tobacco, and meanwhile, the burley tobacco extract also has natural compound rich fragrance including fresh apple fragrance, wine fragrance, roasted cheese fragrance, roasted meat fragrance and the like; the production process has mild conditions, simple steps, easy operation, high effect, low cost, less impurities, obviously reduced burley tobacco characteristic stimulation smell and improved spice quality; the burley tobacco is applied to materials such as formula tobacco shreds, cigarette paper, water-based adhesive and the like in novel heating non-burning tobacco, is coordinated with smoke gas and consistent in style, enhances the herbal fragrance of the tobacco, improves the satisfaction, reduces the irritation, increases the richness of the tobacco fragrance, obviously lightens the irritation smell of burley tobacco, has the fragrance of milk, fresh apple, wine, roasted meat and roasted cheese, is mild in process conditions, is easy to realize, and is simple in operation step.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1
A method for preparing a burley tobacco extract for heating a non-combustible cigarette, comprising the steps of:
(1) pretreatment: crushing burley tobacco leaves into 40-mesh powder by a crusher;
(2) activation of microbial strains: adding active lyophilized powder of Saccharomycopsis fibuligera (Saccharomycopsis fibuligera) into 5 times of sterilized glucose solution with mass concentration of 5%, adding succinic acid, adjusting pH to 1, and activating in 10 deg.C water bath for 10min to obtain strain activating solution;
(3) preparing an immobilized carrier: cleaning and drying burley tobacco stems and bagasse, processing the tobacco stems into fragments with the length of about 1cm, mixing the tobacco stems with the bagasse, and drying after autoclaving to obtain a mixed biological material immobilized carrier;
(4) immobilization treatment of microbial strains: putting a certain amount of the mixed immobilized carrier sterilized and dried in the step (3) into a container, adding the cultured activating solution according to the mass ratio of the immobilized carrier to the microbial strain activating solution of 1:1, and placing the mixture in a refrigerated cabinet at the temperature of 4 +/-0.5 ℃ for fixing for 12 hours to obtain an immobilized strain-carrier;
(5) enzyme immobilization treatment: dissolving asparaginase into an aqueous solution, wherein the mass ratio of the asparaginase to water is 0.01%, adding sodium carbonate to adjust the pH value to 7, uniformly stirring, adding the mixed immobilized carrier obtained in the step (3), and placing the mixed immobilized carrier in a refrigerated cabinet at the temperature of 4 +/-0.5 ℃ for immobilization for 12 hours to obtain an immobilized enzyme-carrier;
(6) two-step fermentation of burley tobacco: uniformly mixing the burley tobacco powder obtained in the step (1) with water of which the mass is 5-15 times that of the burley tobacco powder, adding the immobilized strain-carrier obtained in the step (4), stirring at a constant speed at the temperature of 35 ℃, adding succinic acid to adjust the pH value to 1.0, and fermenting for 1 d; adding ammonia water, adjusting pH to 7, adding the immobilized enzyme-carrier obtained in the step (5), stirring uniformly at 20 ℃, fermenting for 1d, filtering burley tobacco powder residues, and keeping burley tobacco fermentation liquor A;
(7) continuous countercurrent ultrasonic extraction: feeding the burley tobacco residues obtained by filtering in the step (6) into a leaching cabin at the speed of 0.1kg/10min, starting a main machine and an ultrasonic generator, adjusting the temperature to 40 ℃, preparing a propylene glycol aqueous solution with the mass ratio concentration of 50%, adding sodium carbonate, uniformly stirring, adjusting the pH value to 7, adjusting the mass ratio of the material to the liquid ratio to be 1:2, operating for 0.2h from the lower end of the leaching cabin, adjusting a liquid outlet valve to uniformly discharge liquid, controlling the adding flow of a solvent, keeping the liquid level of the leaching cabin balanced, feeding magnesium hydroxide and compound amino acid in a certain proportion when continuous countercurrent ultrasonic extraction reaches 30min, stopping the production process when the continuous countercurrent ultrasonic extraction reaches 90min, discharging slag, and obtaining a leaching solution B;
(8) and (4) combining the fermentation liquor A in the step (6) and the leaching liquor B in the step (7) to obtain the burley tobacco extract.
Further, the saccharomyces cerevisiae fibuligera in the step (2) is food grade, the activity is 10u/mg, and the glucose and the succinic acid are food grade.
Further, the mass ratio of the burley tobacco stems to the bagasse in the step (3) is 1:1, and the mass ratio of the mixed biomaterial-immobilized carrier to the burley tobacco powder is 1: 3.
Further, the sodium carbonate in the step (5) is food grade, and the rotation speed of uniform stirring is 20 rpm.
Further, the magnesium hydroxide used in the step (7) is analytically pure and accounts for 0.1% of the mass of the burley tobacco, the compound amino acids are leucine, isoleucine and cysteine and are in food grade and account for 0.5% of the mass of the burley tobacco, and the mass ratio of the leucine, the isoleucine and the cysteine is 1:0.5: 0.5.
The burley tobacco extract prepared by the method is applied to novel heating non-combustion cigarette formula tobacco shreds, and is flavored according to the mass ratio of 0.001 percent of the heating non-combustion cigarette formula tobacco shreds, so that the cigarette has full tobacco flavor, increased concentration, moderate strength and improved satisfaction, the characteristic strong smell of the burley tobacco is obviously lightened, and the burley tobacco extract also has fresh apple cheese flavor, bouquet, roasted aroma and roasted meat aroma.
Example 2
A method for preparing a burley tobacco extract for heating a non-combustible cigarette, comprising the steps of:
(3) pretreatment: crushing burley tobacco leaves into 100-mesh powder by a crusher;
(2) activation of microbial strains: adding active lyophilized powder of Saccharomycopsis fibuligera (Saccharomycopsis fibuligera) into 12 times of sterilized 15% glucose solution, adding succinic acid, adjusting pH to 3, and activating in 18 deg.C water bath for 20min to obtain strain activating solution;
(3) preparing an immobilized carrier: cleaning and drying burley tobacco stems and bagasse, processing the tobacco stems into fragments with the length of about 1cm, mixing the tobacco stems with the bagasse, and drying after autoclaving to obtain a mixed biological material immobilized carrier;
(4) immobilization treatment of microbial strains: putting a certain amount of the mixed immobilized carrier sterilized and dried in the step (3) into a container, adding the cultured activating solution according to the mass ratio of the immobilized carrier to the microbial strain activating solution of 1:5, and placing the mixture in a refrigerated cabinet at the temperature of 4 +/-0.5 ℃ for fixing for 24 hours to obtain an immobilized strain-carrier;
(5) enzyme immobilization treatment: dissolving asparaginase into an aqueous solution, wherein the mass ratio of the asparaginase to water is 0.01-0.05%, adding sodium carbonate to adjust the pH value to 8.5, uniformly stirring, adding the mixed immobilized carrier obtained in the step (3), and placing the mixed immobilized carrier in a refrigerated cabinet at the temperature of 4 +/-0.5 ℃ for immobilization for 24 hours to obtain an immobilized enzyme-carrier;
(6) two-step fermentation of burley tobacco: uniformly mixing the burley tobacco powder obtained in the step (1) with water of which the mass is 10 times that of the burley tobacco powder, adding the immobilized strain-carrier obtained in the step (4), stirring at a constant speed at the temperature of 45 ℃, adding succinic acid to adjust the pH value to 3.5, and fermenting for 2 d; adding ammonia water, adjusting pH to 8, adding the immobilized enzyme-carrier obtained in the step (5), stirring uniformly at 30 ℃, fermenting for 2d, filtering burley tobacco powder residues, and keeping burley tobacco fermentation liquor A;
(7) continuous countercurrent ultrasonic extraction: feeding the burley tobacco residues obtained by filtering in the step (6) into a leaching cabin at the speed of 0.5kg/10min, starting a main machine and an ultrasonic generator, adjusting the temperature to 50 ℃, preparing a propylene glycol aqueous solution with the concentration of 65% in mass ratio, adding sodium carbonate, uniformly stirring, adjusting the pH value to 8, adjusting the mass ratio of the material to the liquid ratio to be 1:4, operating for 1.6h from the lower end of the leaching cabin, adjusting a liquid outlet valve to uniformly discharge liquid, controlling the adding flow of a solvent, keeping the liquid level of the leaching cabin balanced, feeding and adding magnesium hydroxide and compound amino acid in a certain proportion when continuous countercurrent ultrasonic extraction reaches 45min, stopping the production process when the continuous countercurrent ultrasonic extraction reaches 120min, discharging slag, and obtaining a leaching solution B;
(8) and (4) combining the fermentation liquor A in the step (6) and the leaching liquor B in the step (7) to obtain the burley tobacco extract.
Further, the saccharomyces cerevisiae fibuligera in the step (2) is food grade, the activity is 20u/mg, and the glucose and the succinic acid are food grade.
Further, the mass ratio of the burley tobacco stems to the bagasse in the step (3) is 5.5:1, and the mass ratio of the mixed biomaterial-immobilized carrier to the burley tobacco powder is 1: 6.5.
Further, the sodium carbonate in the step (5) is food grade, and the rotation speed of uniform stirring is 40 rpm.
Further, the magnesium hydroxide used in the step (7) is analytically pure and accounts for 0.5% of the mass of the burley tobacco, the compound amino acids are leucine, isoleucine and cysteine and are in food grade and account for 1.0% of the mass of the burley tobacco, and the mass ratio of the leucine, the isoleucine and the cysteine is 1:1: 1.
The burley tobacco extract prepared by the method is applied to the novel cigarette paper of the cigarette which is not burnt by heating, the burley tobacco extract is coated on the cigarette paper according to 0.01 percent of the mass of the cigarette paper, the cigarette aroma of the novel cigarette is increased, the satisfaction is improved, the aroma is enriched, the irritation is reduced, the irritation smell of the burley tobacco is obviously reduced, and the burley tobacco extract has fresh apple aroma, wine aroma, cheese aroma and barbecue aroma and improves the taste.
Example 3
A method for preparing a burley tobacco extract for heating a non-combustible cigarette, comprising the steps of:
(1) pretreatment: crushing burley tobacco leaves into 200-mesh powder by a crusher;
(2) activation of microbial strains: adding active lyophilized powder of Saccharomycopsis fibuligera (Saccharomycopsis fibuligera) into 20 times of sterilized glucose solution with mass concentration of 30%, adding succinic acid, adjusting pH to 6, and activating in 25 deg.C water bath for 30min to obtain strain activating solution;
(3) preparing an immobilized carrier: cleaning and drying burley tobacco stems and bagasse, processing the tobacco stems into fragments with the length of about 1cm, mixing the tobacco stems with the bagasse, and drying after autoclaving to obtain a mixed biological material immobilized carrier;
(4) immobilization treatment of microbial strains: putting a certain amount of the mixed immobilized carrier sterilized and dried in the step (3) into a container, adding the cultured activating solution according to the mass ratio of the immobilized carrier to the microbial strain activating solution of 1:10, and placing the mixture in a refrigerated cabinet at the temperature of 4 +/-0.5 ℃ for fixing for 36 hours to obtain an immobilized strain-carrier;
(5) enzyme immobilization treatment: dissolving asparaginase into an aqueous solution, wherein the mass ratio of the asparaginase to water is 0.05%, adding sodium carbonate to adjust the pH value to 10, uniformly stirring, adding the mixed immobilized carrier obtained in the step (3), and placing the mixed immobilized carrier in a refrigerated cabinet at the temperature of 4 +/-0.5 ℃ for immobilization for 36 hours to obtain an immobilized enzyme-carrier;
(6) two-step fermentation of burley tobacco: uniformly mixing the burley tobacco powder obtained in the step (1) with water 15 times the mass of the burley tobacco powder, adding the immobilized strain-carrier in the step (4), stirring at a constant speed at the temperature of 55 ℃, adding succinic acid to adjust the pH value to 6.0, and fermenting for 3 d; adding ammonia water, adjusting pH to 9, adding the immobilized enzyme-carrier of step (5), stirring at 40 deg.C, fermenting for 3d, filtering off residue of Burley tobacco, and retaining Burley tobacco fermentation broth A;
(7) continuous countercurrent ultrasonic extraction: feeding the burley tobacco residues obtained by filtering in the step (6) into a leaching cabin at the speed of 1.0kg/10min, starting a main machine and an ultrasonic generator, adjusting the temperature to 60 ℃, preparing a propylene glycol aqueous solution with the concentration of 80% by mass ratio, adding sodium carbonate, uniformly stirring, adjusting the pH value to 9, adjusting the mass ratio of the material to the liquid ratio to be 1:6, operating for 1h from the lower end of the leaching cabin, adjusting a liquid outlet valve to uniformly discharge liquid, controlling the adding flow of a solvent, keeping the liquid level of the leaching cabin balanced, feeding magnesium hydroxide and compound amino acid in a certain proportion when continuous countercurrent ultrasonic extraction reaches 60min, stopping the production process when the continuous countercurrent ultrasonic extraction reaches 150min, discharging slag, and obtaining a leaching solution B;
(8) and (4) combining the fermentation liquor A in the step (6) and the leaching liquor B in the step (7) to obtain the burley tobacco extract.
Further, the saccharomyces cerevisiae fibuligera in the step (2) is food grade, the activity is 10-30 u/mg, and the glucose and the succinic acid are food grade.
Further, the mass ratio of the burley tobacco stems to the bagasse in the step (3) is 10:1, and the mass ratio of the mixed biomaterial-immobilized carrier to the burley tobacco powder is 1: 10.
Further, the sodium carbonate in the step (5) is food grade, and the rotation speed of uniform stirring is 60 rpm.
Further, the magnesium hydroxide used in the step (7) is analytically pure and accounts for 1% of the mass of the burley tobacco, the compound amino acid is leucine, isoleucine and cysteine and is in a food grade and accounts for 1.5% of the mass of the burley tobacco, and the mass ratio of the leucine, the isoleucine and the cysteine is 1:2: 2.
The burley tobacco extract prepared by the method is added into the water-based adhesive for the novel heating non-combustible cigarettes according to the mass ratio of 0.01 percent, is uniformly stirred and applied according to normal process conditions in the wrapping procedure, when the novel cigarette product is heated, the smoke concentration is increased, the tobacco fragrance is enriched, the characteristic smell of the burley tobacco is obviously reduced, and the burley tobacco extract has fresh apple fragrance, wine fragrance, cheese baking fragrance and barbecue fragrance.
Test example 1: comparative evaluation of sensory Effect of applications
The burley tobacco extract and the burley tobacco aqueous extract prepared by adsorption fermentation in examples 1 to 3 were applied to a novel heat non-combustible cigarette, respectively, and subjected to sensory evaluation comparative experiments.
The results show (as shown in table 1): the burley tobacco extract prepared by adsorption fermentation is applied to the formula cut tobacco, cigarette paper and water-based adhesive of novel heating non-burning cigarettes, is coordinated with smoke, keeps consistent cigarette style, enhances the herbal fragrance of the cigarettes, improves the satisfaction, has comfortable cigarette fragrance, has fresh apple fragrance, the fragrance of cheese and barbecue, and the like, reduces the irritation, obviously lightens the characteristic smell of burley tobacco, and improves the taste.
TABLE 1 sensory evaluation of Maryland tobacco leaf fermentation extract in cigarettes
The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. A preparation method of a burley tobacco extract for heating non-combustible cigarettes is characterized by comprising the following steps:
(1) pretreatment: crushing burley tobacco leaves into powder of 40-200 meshes by a crusher;
(2) activation of microbial strains: adding active freeze-dried powder of saccharomyces cerevisiae (Saccharomyces fibuligera) into 5-20 times of sterilized glucose solution with the mass concentration of 5-30%, adding succinic acid, adjusting the pH value to 1-6, and activating in a water bath kettle at 10-25 ℃ for 10-30 min to obtain a strain activation solution;
(3) preparing an immobilized carrier: cleaning and drying burley tobacco stems and bagasse, processing the tobacco stems into 1 cm-long fragments, mixing the fragments with the bagasse, and drying after autoclaving to obtain a mixed biological material immobilized carrier;
(4) immobilization treatment of microbial strains: putting a certain amount of the mixed immobilized carrier sterilized and dried in the step (3) into a container, adding the cultured activating solution according to the mass ratio of the immobilized carrier to the microbial strain activating solution of 1: 1-10, and placing the mixture in a refrigerated cabinet at 4+0.5 ℃ for fixation for 12-36 hours to obtain an immobilized strain-carrier;
(5) enzyme immobilization treatment: dissolving asparaginase into an aqueous solution, wherein the mass ratio of the asparaginase to water is 0.01-0.05%, adding sodium carbonate to adjust the pH value to 7-10, stirring and uniformly mixing, adding the mixed biomaterial immobilized carrier obtained in the step (3), and placing the mixed biomaterial immobilized carrier in a refrigerated cabinet at 4+0.5 ℃ for immobilization for 12-36 hours to obtain an immobilized enzyme-carrier;
(6) two-step fermentation of burley tobacco: uniformly mixing the burley tobacco powder obtained in the step (1) with water of which the mass is 5-15 times that of the burley tobacco powder, adding the immobilized strain-carrier obtained in the step (4), stirring at a constant speed at the temperature of 35-55 ℃, adding succinic acid to adjust the pH value to 1.0-6.0, and fermenting for 1-3 days; adding ammonia water, adjusting the pH value to 7-9, adding the immobilized enzyme-carrier obtained in the step (5), stirring uniformly at the temperature of 20-40 ℃, fermenting for 1-3 d, filtering burley tobacco powder residues, and keeping burley tobacco fermentation liquor A;
(7) continuous countercurrent ultrasonic extraction: feeding the burley tobacco powder residue obtained by filtering in the step (6) into a leaching cabin at the speed of 0.1-1.0 kg/10min, starting a main machine and an ultrasonic generator, adjusting the temperature to 40-60 ℃, preparing a propylene glycol aqueous solution with the mass ratio concentration of 50-80%, adding sodium carbonate, uniformly stirring, adjusting the pH value to 7-9, adjusting the mass ratio of the material to the liquid ratio to be 1: 2-6, operating for 0.2-1 h from the lower end of the leaching cabin, adjusting a liquid outlet valve to uniformly discharge liquid, controlling the adding flow of a solvent, keeping the liquid level of the leaching cabin balanced, feeding magnesium hydroxide and compound amino acid in a certain proportion when continuous countercurrent ultrasonic extraction reaches 30-60 min, stopping the production process when the continuous countercurrent ultrasonic extraction reaches 90-150 min, and discharging slag to obtain a leaching solution B;
(8) and (4) combining the fermentation liquor A in the step (6) and the leaching liquor B in the step (7) to obtain the burley tobacco extract.
2. The method for producing a burley tobacco extract for heat-not-burn cigarettes according to claim 1, wherein: in the step (2), the saccharomyces cerevisiae (Saccharomyces fibuligera) is food-grade, the activity is 10-30 u/mg, and the glucose and the succinic acid are food-grade.
3. The method for producing a burley tobacco extract for heat-not-burn cigarettes according to claim 1, wherein: the mass ratio of the burley tobacco stems to the bagasse in the step (3) is 1-10: 1.
4. The method for producing a burley tobacco extract for heat-not-burn cigarettes according to claim 1, wherein: and (3) the sodium carbonate in the step (5) is food grade, and the stirring speed is 20-60 rpm.
5. The method for producing a burley tobacco extract for heat-not-burn cigarettes according to claim 1, wherein: the magnesium hydroxide used in the step (7) is analytically pure and accounts for 0.1-1% of the mass of the burley tobacco, the compound amino acid is leucine, isoleucine and cysteine and is in a food grade and accounts for 0.5-1.5% of the mass of the burley tobacco, and the mass ratio of the leucine, the isoleucine and the cysteine is 1: 0.5-2.
6. Use of a burley tobacco extract prepared by the method according to any one of claims 1 to 5 in a heated non-burning cigarette, wherein the burley tobacco extract is flavored in a mass ratio of 0.001 to 0.07% to a cut tobacco of the heated non-burning cigarette.
7. Use of a burley tobacco extract prepared according to any one of claims 1 to 5 in a wrapper for a heated non-burning cigarette, the burley tobacco extract being applied in an amount of from 0.01 to 0.7% by mass of the wrapper.
8. The application of the burley tobacco extract prepared by the method of any one of claims 1 to 5 in the water-based adhesive for heating non-burning cigarettes is realized by adding the burley tobacco extract into the water-based adhesive for cigarettes according to the mass ratio of 0.01 to 0.1 percent, uniformly stirring and applying according to normal process conditions in a wrapping process.
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