CN1093753A - L-malic acid production by using new technology of immobilized yellow Bacillus brevis - Google Patents
L-malic acid production by using new technology of immobilized yellow Bacillus brevis Download PDFInfo
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- CN1093753A CN1093753A CN 93110764 CN93110764A CN1093753A CN 1093753 A CN1093753 A CN 1093753A CN 93110764 CN93110764 CN 93110764 CN 93110764 A CN93110764 A CN 93110764A CN 1093753 A CN1093753 A CN 1093753A
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- enzyme
- malic acid
- solution
- fumarate
- immobilized
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- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The present invention adds 5%～20% (weight ratio) gelatin and 1%～5% (weight ratio) Xylo-Mucine in carrageenin.Add water, the back dissolving of heating forms compound organic electrolyte solution and sneak into bacterium mud in the time of 38 ℃～42 ℃, fully stir, carry out mechanical-moulded or manual assembly, in the KCl of 0.3M solution, solidify, solution activation with bile acide and sodium fumarate, immobilized cell is packed in the enzyme post, pass to fumarate.This immobilization process has reduced the zero pour of carrageenin, thereby has reduced enzyme deactivation, has improved the enzyme of immobilized cell and has lived.
Description
The invention belongs to the enzyme engineering field, is a kind of L-malic acid production by using new technology of immobilized yellow Bacillus brevis specifically.
Generally adopt single polymerization organic electrolyte in the immobilized cell technology of production L MALIC ACID at present, as polyacrylamide, alginate calcium, carrageenin etc., up to now, published the fixedly method of brevibacterium flavum and Brevibacterium ammoniagenes of carrageenin, and thought the highest with the transformation efficiency of carrageenin fixed cell.
Carrageenin is a kind of compound of polysaccharide that contains many sulfate groups, has the advantage of good stability as the material of immobilized cell, but its immobilization temperature is too high, and this not only is shaped to immobilization and brings difficulty, and influence is by the activity of embedding cell.
The objective of the invention is to avoid weak point in the above-mentioned prior art and providing a kind of is the immobilization temperature that reduces carrageenin, make in the immobilized cell forming process enzyme non-inactivation in the cell, in carrageenin, add gelatin, after the common dissolving of Xylo-Mucine, the L-malic acid production by using new technology of immobilized yellow Bacillus brevis that the zero pour of this compound organic electrolyte will reduce greatly.
Purpose of the present invention can reach by following measure:
A kind of L-malic acid production by using new technology of immobilized yellow Bacillus brevis, its technology is to sneak into bacterium mud in carrageenan solutions, fully stir, carry out mechanically shape or manual assembly, in the Kcl of 0.3M solution, solidify, after the solution activation with bile acide and sodium fumarate, immobilized cell is packed in the enzyme post, pass to fumarate, the present invention is characterized in that adding 5%～20%(weight percent in carrageenin) gelatin and 1%～5%(weight percent) form compound electrolyte solution behind the Xylo-Mucine mixed dissolution, sneak into fumarate is converted into the thalline of malate after, form immobilized cell (enzyme) and be used to produce L MALIC ACID.
The present invention is further described below in conjunction with embodiment:
1, after quantitatively the mixture of carrageenin and gelatin and Xylo-Mucine adds water, be heated to 80 ℃ of dissolvings, add brevibacterium flavum bacterium mud after 38 ℃～42 ℃ mixing, in the cell shaper, be processed as the ball of φ 5mm, or the manual particle of 5 * 5 * 5mm that is cut into is after curing of Kcl solution and bile acide activate, pack in the 8l immobilized cell reactor, with
=0.23h
-1Flow velocity feed fumarate solution, the transformation period of enzyme is 90～120 days, average conversion is 80%.
2, after quantitatively the mixture of carrageenin and gelatin adds water, be heated to 80 ℃ of dissolvings, add brevibacterium flavum bacterium mud 50g after 38 ℃～40 ℃ mixing, pour tray into, add 0.3MKcl solution and solidify, after dicing, put into pottery pot and activate with bile acide, pack in the immobilized cell post of 250ml, with
=0.23h
-1Flow velocity feed fumarate solution, the transformation period of enzyme is 100 days, average conversion is 75%.
The present invention has following advantage than prior art:
Immobilization process of the present invention has reduced the freezing point of carragheen, thereby has reduced enzyme deactivation, has improved the enzyme of immobilized cell and has lived.
Claims (1)
1, a kind of L-malic acid production by using new technology of immobilized yellow Bacillus brevis, its technology is to sneak into bacterium mud in carrageenan solutions, fully stir, carry out mechanically shape or manual assembly, in the Kcl of 0.3M solution, solidify, after the solution activation with bile acide and sodium fumarate, immobilized cell is packed in the enzyme post, pass to fumarate, the present invention forms compound electrolyte solution it is characterized in that adding 5%～20% (weight percent) gelatin and 1%～5% (weight percent) Xylo-Mucine mixed dissolution in carrageenin after, sneak into fumarate is converted into the thalline of malate after, form immobilized cell (enzyme) and be used to produce L MALIC ACID.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93110764 CN1093753A (en) | 1993-04-16 | 1993-04-16 | L-malic acid production by using new technology of immobilized yellow Bacillus brevis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93110764 CN1093753A (en) | 1993-04-16 | 1993-04-16 | L-malic acid production by using new technology of immobilized yellow Bacillus brevis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1093753A true CN1093753A (en) | 1994-10-19 |
Family
ID=4988595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93110764 Pending CN1093753A (en) | 1993-04-16 | 1993-04-16 | L-malic acid production by using new technology of immobilized yellow Bacillus brevis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1093753A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250977A (en) * | 2011-06-28 | 2011-11-23 | 三峡大学 | Method for preparing L-isoleucine by using immobilized cells |
-
1993
- 1993-04-16 CN CN 93110764 patent/CN1093753A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250977A (en) * | 2011-06-28 | 2011-11-23 | 三峡大学 | Method for preparing L-isoleucine by using immobilized cells |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |