CN109374905A - A kind of device based on isothiocyano label monoclonal antibody - Google Patents
A kind of device based on isothiocyano label monoclonal antibody Download PDFInfo
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- CN109374905A CN109374905A CN201811613293.5A CN201811613293A CN109374905A CN 109374905 A CN109374905 A CN 109374905A CN 201811613293 A CN201811613293 A CN 201811613293A CN 109374905 A CN109374905 A CN 109374905A
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- fixedly connected
- mounting groove
- monoclonal antibody
- isothiocyano
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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Abstract
The invention discloses a kind of devices based on isothiocyano label monoclonal antibody, it includes mounting base, damping, test flume, regulating mechanism, the bottom both ends of mounting base offer the first mounting groove, damping is equipped in first mounting groove, top one end of mounting base offers test flume, test board is equipped in test flume, the inside of mounting base offers the second mounting groove, regulating mechanism is equipped in second mounting groove, the top middle portion of mounting base offers third mounting groove, elevating mechanism is equipped in third mounting groove, the top of elevating mechanism is fixedly connected with material fetching mechanism.The present invention has achieved the effect that damping, has played the role of protection to entire reaction unit by setting damping；By the way that test board is arranged, facilitates placement beaker, support rod, the second return spring and crushing block are provided in test board, protective action also on the one hand is played to beaker while clamping beaker, on the other hand can place the different beaker of model.
Description
Technical field
The invention belongs to mark monoclonal antibody device correlative technology field, monoclonal antibody is marked based on isothiocyano more particularly to one kind
Device.
Background technique
Antibody obtains labelled antibody by enzyme label, ferritin labeling or by colloid gold label, is Electron Microscopic Sample
A kind of method of preparation, the means studied for observing antigenantibody complex aspect.Immunolabelling technique is will be some
Not only it easily measured on the mass signatures to specific antigen or antibody molecule with high susceptibility, and passed through the increasing of these markers
Strong enlarge-effect shows the property and content of antigen or antibody in reaction system.Antibody marks the positioning for being mainly used for antigen
Analysis can also carry out quantitative detection to the antigen in the sample for being contaminated with other a large amount of molecules in some cases.Due to anti-
Body and its corresponding antigens have very high affinity, thus the antibody with marker easy to identify can with positioning analysis antigen, and
It and is a kind of ideal quick inexpensive method for quantitatively determining.In the test of fluorescein isothiocynate (FITC) label, FITC
The principle of labelled antibody be under alkaline condition, the isothiocyano of FITC can in conjunction with the free amino of IgG, formed IgG with it is glimmering
The conjugate of light element.FITC is most common fluorescein, secondly selects TRITC(tetramethylisothiocyanate rhodamine).FITC and
TRITC is common double labelling combination.
In the prior art, if to mark the monoclonal antibody based on isothiocyano, need to use a variety of test apparatuses, very not
It is convenient；And instrument containers are easily damaged during label, and may cause to hurt safely to the operator of label monoclonal antibody
Evil.
Therefore, the application proposes that a kind of device based on isothiocyano label monoclonal antibody solves above-mentioned technical problem.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, the present invention provides a kind of devices based on isothiocyano label monoclonal antibody, in order in base
When isothiocyano marks monoclonal antibody, antibody can be marked, solve technical problem present in background technique.
(2) technical solution
In order to achieve the above object, the present invention is achieved by the following technical programs:
A kind of device based on isothiocyano label monoclonal antibody, including mounting base, damping, test flume, regulating mechanism, the peace
The bottom both ends of dress seat offer the first mounting groove, are equipped with damping, the top of the mounting base in first mounting groove
Portion one end offers test flume, is equipped with test board in the test flume, the inside of the mounting base offers the second mounting groove, institute
It states and is equipped with regulating mechanism in the second mounting groove, the top middle portion of the mounting base offers third mounting groove, the third installation
Elevating mechanism is equipped in slot, the top of the elevating mechanism is fixedly connected with material fetching mechanism, the top other end of the mounting base
It is placed with storing beaker.
Optionally, the damping is made of support leg and supporting leg, and one end of the supporting leg is located at the first installation
In slot and be fixedly connected with the first limited block, the side of first limited block is equipped with arc panel, the side of the arc panel with
First limited block is fixedly connected, and the other side of the arc panel is fitted and connected with the first mounting groove, the other end of the supporting leg
It is fixedly connected with support leg.
Optionally, the top of the test board offers storage slot, is equipped with reaction beaker, the storage in the storage slot
The two sides of slot offer the first fixing groove, and support rod is equipped in first fixing groove, and one end of the support rod is fixed to be connected
It is connected to the second limited block, the other end of the support rod through the first fixing groove and is fixedly connected with crushing block, the support rod
Middle part on the outside of be socketed with the second return spring.
Optionally, one end of second return spring with storage slot be fixedly connected, second return spring it is another
End is fixedly connected with crushing block, and the bottom end of the test board is fixedly connected with first annular sliding block, and the bottom end of the test flume is opened
Equipped with first annular sliding slot, the first annular sliding block is fitted and connected with first annular sliding slot.
Optionally, the regulating mechanism is made of transmission gear and first rotating shaft, and a middle side part of the transmission gear is solid
Surely it is connected with fixed column, one end of the fixed column runs through the second mounting groove and is fixedly connected with the center bottom of test board, described
One end of first rotating shaft is fixedly connected with the first transmission gear, and the first transmission gear engages connection with transmission gear, described
The other end of first rotating shaft is through mounting base and is fixedly connected with steering wheel, and the first rotating shaft passes through first bearing and the second peace
Tankage is flexibly connected.
Optionally, the elevating mechanism is made of sleeve and telescopic rod, and one end of the telescopic rod is located in sleeve, described
The side of telescopic rod offers card slot, and the interior side of the sleeve offers the second fixing groove, sets in second fixing groove
There is fastener, one end of the fastener through the second fixing groove and is inserted into card slot.
Optionally, the other end of the fastener through sleeve and is fixedly connected with pull ring, and the on both sides of the middle of the fastener is equal
It is fixedly connected with third limited block, is socketed with third return spring on the outside of the middle part of the fastener, the third return spring
One end is fixedly connected with third limited block, and the other end of the third return spring is fixedly connected with the second fixing groove.
Optionally, the material fetching mechanism offers on the inside of the fixed disk by fixed disk, rotating disk and reagent needle tubing
Two limit sliding chutes, the rotating disk are fitted and connected with the second limit sliding chutes, and through-hole, the examination are offered in the middle part of the rotating disk
Agent needle tubing is fitted and connected with through-hole, and the circumferentially fixed of the rotating disk is connected with rack gear.
Optionally, the interior side of the fixed disk offers third fixing groove, is equipped with second in the third fixing groove
Shaft, one end of second shaft are fixedly connected with the second transmission gear, and the second transmission wheel and rack engagement connects,
The other end of second shaft is through fixed disk and is fixedly connected with adjusting knob, and the middle part of second shaft passes through second
Bearing is flexibly connected with third fixing groove.
Optionally, the first return spring, one end of first return spring are socketed on the outside of the support midleg
It is fixedly connected with the first mounting groove, the other end of first return spring is fixedly connected with the first limited block.
Optionally, protective pad is fixedly connected on the inside of the crushing block, the protective pad is made of sponge material, described
Reaction beaker is fitted and connected with protective pad, and the reaction beaker has multiple, multiple reaction beaker circular array distributions.
Optionally, the reagent needle tubing and storing beaker at least there are six, multiple storing beaker circular arrays distributions,
The reagent needle tubing is corresponding with storing beaker.
Optionally, the bottom end of the support leg is fixedly connected with gasket, the gasket be it is trapezoidal, the gasket is by rubber material
Material is made.
Optionally, the outer lower end of the sleeve offers the first limit sliding chutes, consolidates on the side wall of the third mounting groove
Surely it is connected with the first position limiting slide block, first position limiting slide block is fitted and connected with the first limit sliding chutes.
Optionally, the upper and lower ends edge of the rotating disk offers the second annular groove, and second annular is sliding
The second ring slider is fitted and connected in slot, second ring slider is fixedly connected with fixed disk.
Optionally, the return spring is cylindroid helical-coil compression spring, and material is stainless steel spring wire, tempering spring
Steel wire, cold drawing carbon spring steel wires or chrome alum spring steel wire etc.；The steel wire diameter d1 of first return spring is 1.5-
2.5mm, length L1 are 10-15cm；The steel wire diameter d2 of second return spring is 0.5-1.3mm, and length L2 is 6-
12cm；The steel wire diameter d3 of the third return spring is 0.5-1.3mm, and length L3 is 6-12cm.
Optionally, in order to guarantee that the device avoids extraneous marking monoclonal antibody from the impact of support zone during the work time
Interference, the steel wire diameter d1 and length L1 of first return spring, the second return spring steel wire diameter d2 and length L2,
Meet following relationship between the steel wire diameter d3 and length L3 of third return spring:
D1:d2:d3=L1:L2:L3.
Optionally, in order to guarantee stability of the regulating mechanism in transmission process, reduce and shake and impact, described first
The material for transmitting gear and transmission gear is carburizing steel, quenched and tempered steel or nitrated steel.The first transmission gear and gear teeth
The tooth face hardness Y1 of wheel is 350-420HBW, and inner hardness Y2 is 290-340HBW, the outer surface of gear can be made to have in this way
Certain hardness improves the bearing capacity of gear, and gear internal can be made to have certain toughness, prevents gear tooth breakage.Institute
The tooth face hardness stated is the hardness of range in from surface to internal 2mm, and the inner hardness is to remove outside tooth face hardness range
Gear material hardness.
Optionally, meet following relationship between the tooth face hardness Y1 and inner hardness Y2:
Y1=α·Y2+Y0；
Wherein α is hardness adjustment factor, value range 0.98-1.4；Y0 is that hardness adjusts radix, value range 10-
20HBW。
Optionally, in order to which the slight vibration for generating regulating mechanism during the work time is filtered out by return spring, prevent
It only influences monoclonal antibody label and causes damages to instrument, steel wire length L2, the third return spring of second return spring
Steel wire length L3, tooth face hardness Y1 and inner hardness Y2 between meet following relationship:
Y1-Y2=μ·L2·L3；
Wherein, μ is coefficient of balance, value range 1.2-3.4.
(3) beneficial effect
Compared with prior art, it is of the invention based on isothiocyano label monoclonal antibody device, have it is following the utility model has the advantages that
(1) device of the invention based on isothiocyano label monoclonal antibody has achieved the effect that damping by setting damping,
Play the role of protection to entire reaction unit, avoids beaker from being impacted and be crushed, substantially increase experimental rig
Safety.
(2) device of the invention based on isothiocyano label monoclonal antibody facilitates placement beaker by setting test board,
It is provided with support rod, the second return spring and crushing block in test board, on the one hand also beaker is played while clamping beaker
Protective action, on the other hand can place the different beaker of model, increase the practicability of device.
(3) device of the invention based on isothiocyano label monoclonal antibody is convenient for by the way that regulating mechanism is arranged when testing
Mobile beaker, so that the beaker for facilitating selection corresponding, keeps away manual-free and take appearance during beaker unexpected, mention for testing crew
Convenience has been supplied, feeding has also been facilitated convenient for reagent needle tubing to be fixed by setting material fetching mechanism, to reduce physical efficiency
Consumption, alleviates operating pressure, improves work efficiency.
(4) device of the invention based on isothiocyano label monoclonal antibody, by the steel wire diameter that the first return spring is arranged
The steel wire diameter d3 and length L3 of d1 and length L1, the steel wire diameter d2 of the second return spring and length L2, third return spring
Range and relationship, guarantee that the device avoids the extraneous interference marked from the impact of support zone to monoclonal antibody during the work time.
(5) device of the invention based on isothiocyano label monoclonal antibody passes through the first transmission gear of setting and transmission gear
Material, hardness range and relationship, guarantee stability of the regulating mechanism in transmission process, reduce vibration and impact, prevent by
Label is influenced in adjusting the focus generated.
(6) device of the invention based on isothiocyano label monoclonal antibody, by the steel wire length that the second return spring is arranged
Relationship between L2, the steel wire length L3 of third return spring, tooth face hardness Y1 and inner hardness Y2, makes regulating mechanism work
The slight vibration generated in the process is filtered out by return spring, is prevented from influencing monoclonal antibody label and be caused damages to instrument.
Detailed description of the invention
Fig. 1 is the device schematic front view of the invention that monoclonal antibody is marked based on isothiocyano.
Fig. 2 is the device schematic top plan view of the invention that monoclonal antibody is marked based on isothiocyano.
Fig. 3 is the device diagrammatic cross-section of the invention that monoclonal antibody is marked based on isothiocyano.
Fig. 4 is the enlarged diagram of structure at A in Fig. 3 of the present invention.
Fig. 5 is the diagrammatic cross-section of the device experiment dish structure of the invention that monoclonal antibody is marked based on isothiocyano.
Fig. 6 is the enlarged diagram of structure at B in Fig. 5 of the present invention.
Fig. 7 is the diagrammatic cross-section for the device descending mechanism structure that monoclonal antibody is marked based on isothiocyano that the present invention rises.
In figure: 1, mounting base；11, the first mounting groove；12, test flume；121, first annular sliding slot；13, the second mounting groove；
14, third mounting groove；141, the first position limiting slide block；15, storing beaker；2, damping；21, support leg；22, supporting leg；23,
First limited block；24, the first return spring；25, arc panel；3, test board；31, slot is stored；32, reaction beaker；33, first is solid
Determine slot；34, support rod；35, the second limited block；36, the second return spring；37, crushing block；38, first annular sliding block；4, it adjusts
Mechanism；41, transmission gear；42, fixed column；43, first rotating shaft；44, the first transmission gear；45, steering wheel；46, first bearing；
5, elevating mechanism；51, sleeve；52, telescopic rod；53, card slot；54, the second fixing groove；55, fastener；56, third limited block；57,
Third return spring；58, pull ring；59, the first limit sliding chutes；6, material fetching mechanism；61, fixed disk；611, the second limit sliding chutes；
612, the second ring slider；613, third fixing groove；614, the second shaft；615, the second transmission gear；616, adjusting knob；
617, second bearing；62, rotating disk；621, the second annular groove；622, rack gear；623, through-hole；63, reagent needle tubing.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the present invention unless specifically defined or limited otherwise, term " setting ", " installation ", " connected ", " connection ",
Terms such as " fixations " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection；It can be mechanical connect
It connects；It can be directly connected, it can also be indirectly connected through an intermediary.For the ordinary skill in the art, may be used
To understand the concrete meaning of above-mentioned term in the present invention as the case may be.
Embodiment 1
According to as shown in figs. 1-7, a kind of device based on isothiocyano label monoclonal antibody, including mounting base 1, damping 2, test
Slot 12, regulating mechanism 4, the bottom both ends of mounting base 1 offer the first mounting groove 11, are equipped with damping machine in the first mounting groove 11
Structure 2, top one end of mounting base 1 offer test flume 12, are equipped with test board 3 in test flume 12, the inside of mounting base 1 offers
Second mounting groove 13, the second mounting groove 13 is interior equipped with regulating mechanism 4, and the top middle portion of mounting base 1 offers third mounting groove 14,
Elevating mechanism 5 is equipped in third mounting groove 14, the top of elevating mechanism 5 is fixedly connected with material fetching mechanism 6, the top of mounting base 1
The other end is placed with storing beaker 15.
Damping 2 is made of support leg 21 and supporting leg 22, and one end of supporting leg 22 is located in the first mounting groove 11 simultaneously
It is fixedly connected with the first limited block 23, the side of the first limited block 23 is equipped with arc panel 25, the side of arc panel 25 and the first limit
Position block 23 is fixedly connected, and the other side of arc panel 25 is fitted and connected with the first mounting groove 11, the other end of supporting leg 22 and support
Foot 21 is fixedly connected, and by the way that damping 2 is arranged, has achieved the effect that damping, the work of protection is played to entire reaction unit
With avoiding beaker from being impacted and be crushed, substantially increase the safety of experimental rig.
The top of test board 3 offers storage slot 31, is equipped with reaction beaker 32 in storage slot 31, the two sides for storing slot 31 are equal
The first fixing groove 33 is offered, is equipped with support rod 34 in the first fixing groove 33, one end of support rod 34 is fixedly connected with the second limit
The other end of position block 35, support rod 34 through the first fixing groove 33 and is fixedly connected with crushing block 37, outside the middle part of support rod 34
Side is socketed with the second return spring 36, and one end of the second return spring 36 is fixedly connected with storage slot 31, the second return spring 36
The other end be fixedly connected with crushing block 37, the bottom end of test board 3 is fixedly connected with first annular sliding block 38, the bottom of test flume 12
End offers first annular sliding slot 121, and first annular sliding block 38 is fitted and connected with first annular sliding slot 121, is tested by setting
Disk 3 facilitates placement beaker, and support rod 34, the second return spring 36 and crushing block 37 are provided in test board 3, is on the one hand existed
Protective action also is played to beaker while clamping beaker, the different beaker of model on the other hand can be placed, increase dress
The practicability set.
Regulating mechanism 4 is made of transmission gear 41 and first rotating shaft 43, and a middle side part of transmission gear 41 is fixedly connected with
One end of fixed column 42, fixed column 42 runs through the second mounting groove 13 and is fixedly connected with the center bottom of test board 3, first rotating shaft 43
One end be fixedly connected with the first transmission gear 44, the first transmission gear 44 engages connection, first rotating shaft 43 with transmission gear 41
The other end through mounting base 1 and be fixedly connected with steering wheel 45, first rotating shaft 43 passes through first bearing 46 and the second mounting groove
13 are flexibly connected, by the way that regulating mechanism 4, the beaker easy to remove when testing is arranged, thus the beaker for facilitating selection corresponding,
It is unexpected by occurring during beaker to keep away manual-free, is provided convenience for testing crew.
Elevating mechanism 5 is made of sleeve 51 and telescopic rod 52, and one end of telescopic rod 52 is located in sleeve 51, telescopic rod 52
Side offers card slot 53, and the interior side of sleeve 51 offers the second fixing groove 54, is equipped with fastener 55 in the second fixing groove 54,
One end of fastener 55 is through the second fixing groove 54 and is inserted into card slot 53, and the other end of fastener 55 through sleeve 51 and is fixedly connected
There is pull ring 58, the on both sides of the middle of fastener 55 is fixedly connected to third limited block 56, is socketed with third on the outside of the middle part of fastener 55
One end of return spring 57, third return spring 57 is fixedly connected with third limited block 56, the other end of third return spring 57
It is fixedly connected with the second fixing groove 54.
Material fetching mechanism 6 offers the second limit by fixed disk 61, rotating disk 62 and reagent needle tubing 63, the inside of fixed disk 61
Sliding slot 611, rotating disk 62 are fitted and connected with the second limit sliding chutes 611, and the middle part of rotating disk 62 offers through-hole 623, reagent needle
Pipe 63 is fitted and connected with through-hole 623, and the circumferentially fixed of rotating disk 62 is connected with rack gear 622, and the interior side of fixed disk 61 opens up
There is a third fixing groove 613, be equipped with the second shaft 614 in third fixing groove 613, one end of the second shaft 614 is fixedly connected with the
Two transmission gears 615, the second transmission gear 615 engage connection with rack gear 622, and the other end of the second shaft 614 runs through fixed disk
61 and it is fixedly connected with adjusting knob 616, the middle part of the second shaft 614 passes through 613 activity of second bearing 617 and third fixing groove
Connection also facilitates feeding by setting material fetching mechanism 6 convenient for reagent needle tubing 63 to be fixed, disappears to reduce physical efficiency
Consumption, alleviates operating pressure, improves work efficiency.
The first return spring 24, one end of the first return spring 24 and the first installation are socketed on the outside of the middle part of supporting leg 22
Slot 11 is fixedly connected, and the other end of the first return spring 24 is fixedly connected with the first limited block 23.
The inside of crushing block 37 is fixedly connected with protective pad, and protective pad is made of sponge material, reaction beaker 32 and protection
Pad is fitted and connected, and reaction beaker 32 has multiple, multiple 32 circular array of reaction beaker distributions, by the way that protective pad is arranged, to anti-
Beaker 32 is answered to play the role of protection.
Reagent needle tubing 63 and storing beaker 15 at least there are six, multiple 15 circular arrays of storing beaker distributions, reagent
Needle tubing 63 is corresponding with storing beaker 15, is arranged in this way, can operate a variety of tests simultaneously, improve work efficiency.
The bottom end of support leg 21 is fixedly connected with gasket, gasket be it is trapezoidal, gasket is made of rubber material, passes through setting pad
Piece plays the role of damping, plays protective action to device.
The outer lower end of sleeve 51 offers the first limit sliding chutes 59, and is fixedly connected on the side wall of third mounting groove 14
One position limiting slide block 141, the first position limiting slide block 141 are fitted and connected with the first limit sliding chutes 59.
The upper and lower ends edge of rotating disk 62 offers the second annular groove 621, fitting in the second annular groove 621
It is connected with the second ring slider 612, the second ring slider 612 is fixedly connected with fixed disk 61.
In summary: should be based on device of isothiocyano label monoclonal antibody, at work, a storing beaker wherein first
With carbonate buffer solution (PH9.3, Na in 152CO38.6g, NaHCO317.3g, distilled water 1000ml) adjustment antibody concentration is extremely
10mg/ml is put into 0.2ml DMSO in yet another storing beaker 15, is then added in 1mg FITC to 0.2ml DMSO, so
The height of elevating mechanism 5 is turned up afterwards, when adjusting, first pulls pull ring 58, pull ring 58 is displaced outwardly with moved fastener 55, works as fastener
After 55 separate with card slot 53, telescopic rod 52 is pulled up, then unclamps pull ring 58, third limited block 56 is in third return spring 57
Band moved fastener 55 moves inward under the action of elastic force, when fastener 55 and the clamping of another card slot 53, just adjusts elevating mechanism 5
Height, material fetching mechanism 6 is directed at storing beaker 15, at this moment again by the height of elevating mechanism 5 by then rotary lifting mechanism 5
It turns down, is inserted into reagent needle tubing 63 in corresponding storing beaker 15, one of reagent needle tubing 63 is made to extract 5ml carbonate buffer
Liquid extracts another reagent needle tubing 63 by the lysate of 1mg FITC and 0.2ml DMSO, then again rotates material fetching mechanism 6
To 3 top of test board, two kinds of liquid are put into reaction beaker 32, then agitating solution, then places a device into shady place
Room temperature is protected from light effect 2 hours, and cross-linking agent is removed free fluorescein through Sephadex G25 or G50 column chromatography, then with being in charge of
The antibody that first peak is label is collected, finally identifies the conjugate quality of FITC, generally, FITC is 3 to the molar ratio of antibody:
It is suitable for tissue section strain when 1, is suitable for cell suspension dyeing when being 5-6:1, with the various antigens of Labeled Antibodies Staining, measurement
The degree of its specific stain titre and non-specific dyeing, labelled antibody are preferably stored in 4 DEG C of environment, add NaN3 can be with anti-corrosion.
In order to guarantee that the device avoids the extraneous interference marked from the impact of support zone to monoclonal antibody, institute during the work time
The steel wire diameter d1 and length L1 of the first return spring stated, the steel wire diameter d2 and length L2 of the second return spring, third are returned
Meet following relationship between the steel wire diameter d3 and length L3 of power spring:
D1:d2:d3=L1:L2:L3.
In order to guarantee stability of the regulating mechanism in transmission process, reduce vibration and impact, the first transmission tooth
The material of wheel and transmission gear is carburizing steel, quenched and tempered steel or nitrated steel.The tooth of first the transmission gear and transmission gear
Surface hardness Y1 is 350-420HBW, and inner hardness Y2 is 290-340HBW, and the outer surface of gear can in this way had centainly
Hardness improves the bearing capacity of gear, and gear internal can be made to have certain toughness, prevents gear tooth breakage.The tooth
Surface hardness is the hardness of range in from surface to internal 2mm, and the inner hardness is the gear removed outside tooth face hardness range
The hardness of material.
Embodiment 2
A kind of device based on isothiocyano label monoclonal antibody, including mounting base 1, damping 2, test flume 12, regulating mechanism 4,
The bottom both ends of mounting base 1 offer the first mounting groove 11, are equipped with damping 2, the top of mounting base 1 in the first mounting groove 11
Portion one end offers test flume 12, is equipped with test board 3 in test flume 12, and the inside of mounting base 1 offers the second mounting groove 13, the
It is equipped with regulating mechanism 4 in two mounting grooves 13, the top middle portion of mounting base 1 offers third mounting groove 14, in third mounting groove 14
Equipped with elevating mechanism 5, the top of elevating mechanism 5 is fixedly connected with material fetching mechanism 6, and the top other end of mounting base 1 is placed with storage
Expect beaker 15.
Damping 2 is made of support leg 21 and supporting leg 22, and one end of supporting leg 22 is located in the first mounting groove 11 simultaneously
It is fixedly connected with the first limited block 23, the side of the first limited block 23 is equipped with arc panel 25, the side of arc panel 25 and the first limit
Position block 23 is fixedly connected, and the other side of arc panel 25 is fitted and connected with the first mounting groove 11, the other end of supporting leg 22 and support
Foot 21 is fixedly connected, and by the way that damping 2 is arranged, has achieved the effect that damping, the work of protection is played to entire reaction unit
With avoiding beaker from being impacted and be crushed, substantially increase the safety of experimental rig.
The top of test board 3 offers storage slot 31, is equipped with reaction beaker 32 in storage slot 31, the two sides for storing slot 31 are equal
The first fixing groove 33 is offered, is equipped with support rod 34 in the first fixing groove 33, one end of support rod 34 is fixedly connected with the second limit
The other end of position block 35, support rod 34 through the first fixing groove 33 and is fixedly connected with crushing block 37, outside the middle part of support rod 34
Side is socketed with the second return spring 36, and one end of the second return spring 36 is fixedly connected with storage slot 31, the second return spring 36
The other end be fixedly connected with crushing block 37, the bottom end of test board 3 is fixedly connected with first annular sliding block 38, the bottom of test flume 12
End offers first annular sliding slot 121, and first annular sliding block 38 is fitted and connected with first annular sliding slot 121, is tested by setting
Disk 3 facilitates placement beaker, and support rod 34, the second return spring 36 and crushing block 37 are provided in test board 3, is on the one hand existed
Protective action also is played to beaker while clamping beaker, the different beaker of model on the other hand can be placed, increase dress
The practicability set.
Regulating mechanism 4 is made of transmission gear 41 and first rotating shaft 43, and a middle side part of transmission gear 41 is fixedly connected with
One end of fixed column 42, fixed column 42 runs through the second mounting groove 13 and is fixedly connected with the center bottom of test board 3, first rotating shaft 43
One end be fixedly connected with the first transmission gear 44, the first transmission gear 44 engages connection, first rotating shaft 43 with transmission gear 41
The other end through mounting base 1 and be fixedly connected with steering wheel 45, first rotating shaft 43 passes through first bearing 46 and the second mounting groove
13 are flexibly connected, by the way that regulating mechanism 4, the beaker easy to remove when testing is arranged, thus the beaker for facilitating selection corresponding,
It is unexpected by occurring during beaker to keep away manual-free, is provided convenience for testing crew.
Elevating mechanism 5 is made of sleeve 51 and telescopic rod 52, and one end of telescopic rod 52 is located in sleeve 51, telescopic rod 52
Side offers card slot 53, and the interior side of sleeve 51 offers the second fixing groove 54, is equipped with fastener 55 in the second fixing groove 54,
One end of fastener 55 is through the second fixing groove 54 and is inserted into card slot 53, and the other end of fastener 55 through sleeve 51 and is fixedly connected
There is pull ring 58, the on both sides of the middle of fastener 55 is fixedly connected to third limited block 56, is socketed with third on the outside of the middle part of fastener 55
One end of return spring 57, third return spring 57 is fixedly connected with third limited block 56, the other end of third return spring 57
It is fixedly connected with the second fixing groove 54.
Material fetching mechanism 6 offers the second limit by fixed disk 61, rotating disk 62 and reagent needle tubing 63, the inside of fixed disk 61
Sliding slot 611, rotating disk 62 are fitted and connected with the second limit sliding chutes 611, and the middle part of rotating disk 62 offers through-hole 623, reagent needle
Pipe 63 is fitted and connected with through-hole 623, and the circumferentially fixed of rotating disk 62 is connected with rack gear 622, and the interior side of fixed disk 61 opens up
There is a third fixing groove 613, be equipped with the second shaft 614 in third fixing groove 613, one end of the second shaft 614 is fixedly connected with the
Two transmission gears 615, the second transmission gear 615 engage connection with rack gear 622, and the other end of the second shaft 614 runs through fixed disk
61 and it is fixedly connected with adjusting knob 616, the middle part of the second shaft 614 passes through 613 activity of second bearing 617 and third fixing groove
Connection also facilitates feeding by setting material fetching mechanism 6 convenient for reagent needle tubing 63 to be fixed, disappears to reduce physical efficiency
Consumption, alleviates operating pressure, improves work efficiency.
The first return spring 24, one end of the first return spring 24 and the first installation are socketed on the outside of the middle part of supporting leg 22
Slot 11 is fixedly connected, and the other end of the first return spring 24 is fixedly connected with the first limited block 23.
The inside of crushing block 37 is fixedly connected with protective pad, and protective pad is made of sponge material, reaction beaker 32 and protection
Pad is fitted and connected, and reaction beaker 32 has multiple, multiple 32 circular array of reaction beaker distributions, by the way that protective pad is arranged, to anti-
Beaker 32 is answered to play the role of protection.
Reagent needle tubing 63 and storing beaker 15 at least there are six, multiple 15 circular arrays of storing beaker distributions, reagent
Needle tubing 63 is corresponding with storing beaker 15, is arranged in this way, can operate a variety of tests simultaneously, improve work efficiency.
The bottom end of support leg 21 is fixedly connected with gasket, gasket be it is trapezoidal, gasket is made of rubber material, passes through setting pad
Piece plays the role of damping, plays protective action to device.
The outer lower end of sleeve 51 offers the first limit sliding chutes 59, and is fixedly connected on the side wall of third mounting groove 14
One position limiting slide block 141, the first position limiting slide block 141 are fitted and connected with the first limit sliding chutes 59.
The upper and lower ends edge of rotating disk 62 offers the second annular groove 621, fitting in the second annular groove 621
It is connected with the second ring slider 612, the second ring slider 612 is fixedly connected with fixed disk 61.
The return spring is cylindroid helical-coil compression spring, material be stainless steel spring wire, tempering spring steel wire,
Cold drawing carbon spring steel wires or chrome alum spring steel wire etc.；The steel wire diameter d1 of first return spring is 1.5-2.5mm,
Length L1 is 10-15cm；The steel wire diameter d2 of second return spring is 0.5-1.3mm, and length L2 is 6-12cm；It is described
Third return spring steel wire diameter d3 be 0.5-1.3mm, length L3 be 6-12cm.
In order to guarantee that the device avoids the extraneous interference marked from the impact of support zone to monoclonal antibody, institute during the work time
The steel wire diameter d1 and length L1 of the first return spring stated, the steel wire diameter d2 and length L2 of the second return spring, third are returned
Meet following relationship between the steel wire diameter d3 and length L3 of power spring:
D1:d2:d3=L1:L2:L3.
In order to guarantee stability of the regulating mechanism in transmission process, reduce vibration and impact, the first transmission tooth
The material of wheel and transmission gear is carburizing steel, quenched and tempered steel or nitrated steel.The tooth of first the transmission gear and transmission gear
Surface hardness Y1 is 350-420HBW, and inner hardness Y2 is 290-340HBW, and the outer surface of gear can in this way had centainly
Hardness improves the bearing capacity of gear, and gear internal can be made to have certain toughness, prevents gear tooth breakage.The tooth
Surface hardness is the hardness of range in from surface to internal 2mm, and the inner hardness is the gear removed outside tooth face hardness range
The hardness of material.
Meet following relationship between the tooth face hardness Y1 and inner hardness Y2:
Y1=α·Y2+Y0；
Wherein α is hardness adjustment factor, value range 0.98-1.4；Y0 is that hardness adjusts radix, value range 10-
20HBW。
In order to which the slight vibration for generating regulating mechanism during the work time is filtered out by return spring, prevent from influencing single
Anti- to mark and cause damages to instrument, steel wire length L2, the steel wire of third return spring of second return spring are long
Meet following relationship between degree L3, tooth face hardness Y1 and inner hardness Y2:
Y1-Y2=μ·L2·L3；
Wherein, μ is coefficient of balance, value range 1.2-3.4.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features,
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (7)
1. a kind of device based on isothiocyano label monoclonal antibody, including mounting base (1), damping (2), test flume (12), tune
It saves mechanism (4), it is characterised in that: the bottom both ends of the mounting base (1) offer the first mounting groove (11), first peace
Damping (2) are equipped in tankage (11), top one end of the mounting base (1) offers test flume (12), the test flume
(12) test board (3) are equipped in, the inside of the mounting base (1) offers the second mounting groove (13), second mounting groove
(13) regulating mechanism (4) are equipped in, the top middle portion of the mounting base (1) offers third mounting groove (14), the third peace
Elevating mechanism (5) are equipped in tankage (14), the top of the elevating mechanism (5) is fixedly connected with material fetching mechanism (6), the installation
The top other end of seat (1) is placed with storing beaker (15).
2. a kind of device based on isothiocyano label monoclonal antibody according to claim 1, it is characterised in that: the damping machine
Structure (2) is made of support leg (21) and supporting leg (22), and one end of the supporting leg (22) is located in the first mounting groove (11) simultaneously
It is fixedly connected with the first limited block (23), the side of first limited block (23) is equipped with arc panel (25).
3. a kind of device based on isothiocyano label monoclonal antibody according to claim 1 or 2, it is characterised in that: the tune
Section mechanism (4) is made of transmission gear (41) and first rotating shaft (43), and a middle side part of the transmission gear (41) is fixedly connected
Have fixed column (42), one end of the fixed column (42) runs through the second mounting groove (13) and is fixedly connected with the bottom end of test board (3)
Middle part, one end of the first rotating shaft (43) are fixedly connected with the first transmission gear (44).
4. a kind of device based on isothiocyano label monoclonal antibody according to claim 1-3, it is characterised in that: institute
It states elevating mechanism (5) to be made of sleeve (51) and telescopic rod (52), one end of the telescopic rod (52) is located in sleeve (51), institute
The side for stating telescopic rod (52) offers card slot (53), and the interior side of the sleeve (51) offers the second fixing groove (54),
Fastener (55) are equipped in second fixing groove (54).
5. a kind of device based on isothiocyano label monoclonal antibody according to claim 1, it is characterised in that: the support leg
(21) bottom end is fixedly connected with gasket, the gasket be it is trapezoidal, the gasket is made of rubber material.
6. a kind of device based on isothiocyano label monoclonal antibody according to claim 1, it is characterised in that: the sleeve
(51) outer lower end offers the first limit sliding chutes (59), is fixedly connected with first on the side wall of the third mounting groove (14)
Position limiting slide block (141).
7. a kind of device based on isothiocyano label monoclonal antibody according to claim 1, it is characterised in that: the rotating disk
(62) upper and lower ends edge offers the second annular groove (621), is fitted and connected in second annular groove (621)
There are the second ring slider (612).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811613293.5A CN109374905A (en) | 2018-12-27 | 2018-12-27 | A kind of device based on isothiocyano label monoclonal antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811613293.5A CN109374905A (en) | 2018-12-27 | 2018-12-27 | A kind of device based on isothiocyano label monoclonal antibody |
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| Publication Number | Publication Date |
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| CN109374905A true CN109374905A (en) | 2019-02-22 |
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ID=65372411
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811613293.5A Pending CN109374905A (en) | 2018-12-27 | 2018-12-27 | A kind of device based on isothiocyano label monoclonal antibody |
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