CN109355401A - A kind of relevant gene loci library of hair and its application - Google Patents
A kind of relevant gene loci library of hair and its application Download PDFInfo
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Abstract
The invention belongs to technical field of gene detection, it provides a kind of relevant gene loci library of hair and its application method, gene loci library includes SRD5A2 gene, 2-V89L gene, AR gene, DHT gene, FGF-5 gene, MAPK gene, HIF gene, BCMO1 gene, SELL gene, DDB2 gene, IL-6 gene, TNF-alpha gene, Edar gene, VDR gene, CYP450 gene, DAG gene, MCR1 gene, XRCC1 gene, ERCC2 gene, KITLG gene, TYR gene, TYRP1 gene, OCA2 gene, Wnt gene and beta-catenin gene；Site library is comprehensive, and the root of hair problem is caused from the reflection of gene level.The scoring for setting polymorphic site, can simply evaluate hair state, evaluation result is accurate, provides scheme for individual nursing hair.
Description
Technical field
The invention belongs to technical field of gene detection more particularly to a kind of relevant gene loci library of hair and its applications.
Background technique
Nowadays, many people can select skin care item according to oneself skin quality or skin problem, but few people are taking care of hair
Aspect also uses identical theory.Which is strong for advertisement, which is promoted and just buys which product by force almost at public common fault.It is practical
On, according to different hair quality demands, need to carry out the nursing of accurate " to the ill ".No matter man or Ms, we have been spent largely
Time arranges the hair of oneself, however some people possess a pitch-black beautiful healthy hair, and some people's oligotrichosis, hair are dry
It is withered, there are various hairs.Hair condition is bad to be caused by many factors, including gene genetic factor, human hormone's water
Gentle health status etc..Therefore, even if the characterization of hair condition is consistent, it is also very likely derived from different factors, for example, dandruff
Fat secretion is likely to be excessively it could also be possible that fat secretion is very few caused, if using the anti-dandruff of " oil of dispelling " type simply
It is more serious that product only results in problem.
Chinese patent CN1763088B discloses a kind of and human hair and tooth development GAP-associated protein GAP and its encoding gene, institute
The protein for being mutated into glycine residue by arginine residues from the 65th amino acids residue of aminoterminal that albumen is EDA is stated, is compiled
The gene of the code albumen is the congenital related gene for lacking tooth and closing companion's curly headed clinical phenotypes of the Mongols, by detecting the base
Cause carries out pre-natal diagnosis, improves the health of the people.The patent does not refer to the relationship between genetic test and hair state, does not have yet
The method for being provided with the root problem that judgement causes hair to be not in good state.
Chinese patent CN102549156A discloses hair shape susceptibility gene, assert first relevant to hair style
Gene pleiomorphism carries out SNP marker, then explores the relationship between hair style and the portion's gene expression of hair root of hair, determines hair
The associated hair formation mechenism of shape is sent out, realizes the method that hair style is adjusted.The research direction of the patent is, influences hair
The tumor susceptibility gene for sending out shape improves hair style by gene expression regulation or administration；It is not related to hair state issues
Research.
Gene determines hair follicle growth, fat secretion, sensibility of people etc., and regulating and controlling includes egg needed for the hairs such as keratin
The difference that white synthesis, the exactly critical difference of these important genes determine each human hair.It, can be with by genetic test
Specify hair symptom and its producing cause.These gene informations are grasped, science, personalized hairdressing method or production can be selected
Product are targetedly improved and are intervened, and realize targeting precisely hair care.But be which gene influences hair state on earth,
There is not been reported, more has no about by genetic test, carries out the correlative study of hair Problem judgment, also fail to really realize
" customization of amount hair " has one's hair wash, the product of hair care and hair care, scientific maintenance hair.
Summary of the invention
The purpose of the present invention is to provide a kind of relevant gene loci library of hair and its application, the gene loci library provided
25 kinds of genes relevant to hair problem are covered, each gene contains one or more gene pleiomorphism positions for causing hair problem
Point collectively constitutes gene loci library.Hair state evaluation is carried out using the gene loci library, going out for hair problem can not only be prevented
It is existing, moreover it is possible to which that precisely discovery causes the basic reason of hair problem, realizes the scientific maintenance of hair.
Specifically, in view of the deficiencies of the prior art, the present invention provides the following technical scheme that
On the one hand, the present invention provides a kind of relevant gene loci libraries of hair, including SRD5A2 gene, 2-V89L base
Cause, AR gene, DHT gene, FGF-5 gene, MAPK gene, HIF gene, BCMO1 gene, SELL gene, DDB2 gene, IL-6
Gene, TNF-alpha gene, Edar gene, VDR gene, CYP450 gene, DAG gene, MCR1 gene, XRCC1 gene,
ERCC2 gene, KITLG gene, TYR gene, TYRP1 gene, OCA2 gene, Wnt gene and beta-catenin gene.
Preferably, the SRD5A2 gene, 2-V89L gene, AR gene, DHT gene, FGF-5 gene and hormone-type alopecia
Correlation, the MAPK gene and HIF gene are related to disorders of lipid metabolism, and the BCMO1 gene is related to fat secretion, described
SELL gene and DDB2 gene are related to bacterium neurological susceptibility, and IL-6 gene, TNF-alpha gene are related to inflammatory factor secretion,
Edar gene and VDR gene are bad related to hair follicle development, and CYP450 gene is easily destroyed related, DAG gene and angle to hair follicle
Protein secretion is related, and MCR1 gene, XRCC1 gene and ERCC2 gene are related to anti-uv-ray, KITLG gene, TYR base
Because, TYRP1 gene and OCA2 gene are related to congenital white hair, Wnt gene and beta-catenin gene and Aging white hair phase
It closes.
Preferably, SRD5A2 gene polynorphisms site is rs523349, rs1691053 and rs700519,2-
V89L gene polynorphisms site be rs1799941 and rs6259, AR gene polynorphisms site be rs722208 and
Rs1204038, DHT gene polynorphisms site are rs1691053 and rs9287638, and FGF-5 gene polynorphisms site is
Rs2981582 and rs13317.
Preferably, MAPK gene polynorphisms site be rs13317, rs12701937 and rs56234898, it is described
HIF gene polynorphisms site is rs11549465 and rs2057482；BCMO1 gene polynorphisms site is
Rs7501331, rs119478057 and rs12934922.
Preferably, SELL gene polynorphisms site be rs2229569 and rs4987310, DDB2 gene it is polymorphic
Property site be rs747650 and rs7531806, IL-6 gene polynorphisms site be rs1474347, rs1548216 and
Rs1800795, TNF-alpha gene polynorphisms site are rs1800629 and rs361525, the Edar gene polynorphisms
Site is rs3827760 and rs121908450, and VDR gene polynorphisms site is rs1544410 and rs2228570, described
CYP450 gene polynorphisms site is rs11636419 and rs17861162, and DAG gene polynorphisms site is
Rs4625 and rs12583, the MCR1 gene is also related to melanosome synthesis, polymorphic site be rs2228479 and
Rs1805008, XRCC1 gene polynorphisms site are rs25487 and rs1799782, and ERCC2 gene polynorphisms site is
Rs13181, KITLG gene polynorphisms site are rs755383 and rs794726675, TYR gene polynorphisms site
For rs1393350 and rs1126809, TYRP1 gene polynorphisms site is rs2733832, OCA2 gene polynorphisms site
For rs1800401 and rs1800414, Wnt gene polynorphisms site is rs7349332 and rs7775, β-catenin base
The polymorphic site of cause is rs524952 and rs2327832.
Preferably, when MCR1 gene polynorphisms site rs2228479 and rs1805008 are respectively AA and TT, uvioresistant
Line ability is poor, and hair fragility is easily broken；When rs2228479 and rs1805008 are respectively GG and CC, melanosome synthesis is insufficient, occurs
Congenital white hair.
On the other hand, application of the relevant gene loci library of aforementioned hair in hair state evaluation, comprising the following steps:
S1. the gene of user is obtained；
S2. the gene in gene gene loci relevant with hair library that step S1 is obtained is compared, determines gene
The genotype of polymorphic site calculates scoring；
S3. according to the hair state for determining user that scores.
Preferably, the method that scoring is calculated described in step S2 is that the scoring of each polymorphic site of each gene is folded
Add, wherein the standards of grading of each gene polymorphism sites are as follows:
SRD5A2 gene polynorphisms site rs523349 mutually should be 33 points, 16.5 points and 1 with the scoring of GG for CC, CG
Point, rs1691053 AA, AG mutually should be 33 points, 16.5 points and 1 point with the scoring of GG, and rs700519 is the scoring of TT CT CC
It mutually should be 33 points, 16.5 points and 1 point, scoring, the higher activity for indicating 5 alpha-reductases is higher, and hormone-type alopecia is more serious；
2-V89L gene polynorphisms site rs1799941 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG,
Rs6259 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG, and the higher expression 3male hormone secretion level that scores is higher,
Hormone-type alopecia is more serious；
AR gene polynorphisms site rs722208 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG,
Rs1204038 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT, and the higher expression AR acceptor levels that score are higher, swash
Plain type alopecia is more serious；
DHT gene polynorphisms site rs1691053 mutually should be 50 points, 25 points and 1 point with the scoring of AA for GG, AG,
Rs9287638 mutually should be 50 points, 25 points and 1 point with the scoring of CC for AA, AC, and the higher expression DHT acceptor levels that score are higher, swash
Plain type alopecia is more serious；
FGF-5 gene polynorphisms site rs2981582 mutually should be 50 points, 25 points and 1 point with the scoring of CC for TT, CT,
Rs13317 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT, score higher expression FGF-5 gene expression dose more
Height, hormone-type alopecia are more serious；
MAPK gene is related to disorders of lipid metabolism, and polymorphic site rs13317 mutually should be 50 with the scoring of CC for TT, CT
Point, 25 points and 1 point, rs12701937 CC, CT and the scoring of TT mutually should be 50 points, 25 points and 1 point, rs56234898 AA,
AG mutually should be 50 points, 25 points and 1 point with the scoring of GG, and the higher expression lipid-metabolism that scores is stronger；
HIF gene is related to disorders of lipid metabolism, and polymorphic site rs11549465 is that CC, CT mutually should be with the scoring of TT
50 points, 25 points and 1 point, rs2057482 CC, CT mutually should be 50 points, 25 points and 1 point with the scoring of TT, and score higher expression rouge
Matter metaboilic level is higher；
BCMO1 gene is related to fat secretion, and polymorphic site rs7501331 mutually should be 33 with the scoring of TT for CC, CT
Divide, 16.5 points and 1, rs119478057 AA, AT mutually should be 33 points, 16.5 points and 1 point with the scoring of TT, and rs12934922 is
TT, AT mutually should be 33 points, 16.5 points and 1 point with the scoring of AA, and the higher expression fat secretion level that scores is higher.
In another aspect, application of the relevant gene loci library of aforementioned hair in hair state evaluation, which is characterized in that packet
Include following steps:
S1. the gene of user is obtained；
S2. the gene in gene gene loci relevant with hair library that step S1 is obtained is compared, determines gene
The genotype of polymorphic site calculates scoring；
S3. according to the hair state for determining user that scores, the highest gene that scores is the determinant of hair state；
Wherein, the method for calculating scoring is the scoring superposition of each polymorphic site of each gene, same polymorphic position
The same gene type of point contains there are many when scoring, is calculated with more high score.
Preferably, the standards of grading of each gene polymorphism sites are as follows:
SELL gene polynorphisms site rs2229569 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG,
Rs4987310 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG, and higher expression of scoring is easier by bacterium sense
Dye；
DDB2 gene polynorphisms site rs747650 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG,
Rs7531806 mutually should be 25 points, 12.5 points and 1 point with the scoring of GG for AA, AG, and higher expression of scoring is easier by bacterium sense
Dye；
IL-6 gene polynorphisms site rs1474347 mutually should be 20 points, 10 points and 1 point with the scoring of GG for TT, GT,
Rs1548216 mutually should be 20 points, 10 points and 1 point with the scoring of CC for GG, GC, and the scoring of rs1800795 GG, GC and CC are corresponding
It is 20 points, 10 points and 1 point, the higher expression inflammatory factor secretion level that scores is higher；
TNF-alpha gene polynorphisms site rs1800629 mutually should be 20 points, 10 points and 1 with the scoring of GG for AA, AG
Point, rs361525 GG, AG mutually should be 20 points, 10 points and 1 point with the scoring of AA, and score higher expression inflammatory factor secretion level
It is higher；
Edar gene polynorphisms site is that rs3827760 is that CC, CT with the scoring of TT mutually should be 50 points, 25 points and 1 point,
Rs121908450 mutually should be 50 points, 25 points and 1 point with the scoring of AA for GG, AG, and score higher expression hair follicle growth signal path
Gene inactivation is more serious, and hair follicle development is bad；
VDR gene polynorphisms site rs1544410 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG,
Rs2228570 mutually should be 50 points, 25 points and 1 point with the scoring of CC for TT, CT, score higher expression vitamin D receptor level more
Low, hair follicle development is bad；
CYP450 gene polynorphisms site rs11636419 mutually should be 50 points, 25 points and 1 with the scoring of AA for CC, AG
Point, rs17861162 CC, CG mutually should be 50 points, 25 points and 1 point with the scoring of GG, and the higher expression hair follicle that scores protects aromatase enzyme
Horizontal lower, hair follicle is easily destroyed；
DAG gene polynorphisms site rs4625 mutually should be 50 points, 25 points and 1 point with the scoring of CC for TT, CT,
Rs12583 mutually should be 50 points, 25 points and 1 point with the scoring of GG for TT, GT, and the higher expression keratin secretion level that scores is lower；
When MCR1 gene evaluates anti-uv-ray, polymorphic site rs2228479 is that AA, AG mutually should be with the scoring of GG
20 points, 10 points and 1 point, rs1805008 TT, CT mutually should be 20 points, 10 points and 1 point with the scoring of CC, and higher expression of scoring is anti-
UV resistance is poorer；When evaluating melanosome synthesis, polymorphic site rs2228479 mutually should be 1 with the scoring of GG for AA, AG
Divide, 25 points and 50 points, rs1805008 TT, CT mutually should be 1 point, 25 points and 50 points with the scoring of CC, and score higher expression melanocyte
Body synthesis is horizontal lower, congenital white hair occurs；
XRCC1 gene polynorphisms site rs25487 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG,
Rs1799782 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT, and the higher expression anti-uv-ray that scores is poorer；
ERCC2 gene polynorphisms site rs13181 mutually should be 20 points, 10 points and 1 point with the scoring of TT for GG, GT, comment
Divide higher expression anti-uv-ray poorer；
KITLG gene polynorphisms site rs755383 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT,
Rs794726675 mutually should be 50 points, 25 points and 1 point with the scoring of TT for AA, AT, and the higher expression melanocyte proliferation that scores is horizontal
Lower, congenital white hair is more serious；
TYR gene polynorphisms site rs1393350 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG,
Rs1126809 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG, and the higher expression tyrosinase activity that scores is lower,
Congenital white hair is more serious；
TYRP1 gene polynorphisms site rs2733832 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT,
Scoring, higher expression tyrosinase acceptor levels are lower, and congenital white hair is more serious；
OCA2 gene polynorphisms site rs1800401 mutually should be 20 points, 10 points and 1 point with the scoring of GG for AA, AG,
Rs1800414 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT, and the higher expression tyrosinase activity that scores is lower,
Congenital white hair is more serious；
Wnt gene polynorphisms site rs7349332 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT,
Rs7775 mutually should be 50 points, 25 points and 1 point with the scoring of GG for CC, CG, the higher gene expression for indicating Wnt signal path of scoring
Horizontal lower, Aging white hair is more serious；
The polymorphic site rs524952 of beta-catenin gene mutually should be 50 points, 25 points and 1 with the scoring of TT for AA, AT
Point, rs2327832 AA, AG mutually should be 50 points, 25 points and 1 point with the scoring of GG, higher expression melanocyte mother cell differentiation of scoring
Suppressed degree is stronger, and Aging white hair is more serious.
Compared with prior art, it effect of the invention and has an advantage that:
1. hair related gene loci provided by the invention library covers 25 kinds of genes relevant to hair problem, gene is different
Often expressing corresponding hair problem includes alopecia, brittle failure, the short-tempered, dandruff that dries up, greasy and white hair etc., the research to hair-like state
Very comprehensively.
2. gene polymorphism sites (SNP site) are from the big data of US National Biotechnology Information center (NCBI)
Library, data source is wide and has scientific basis；Therefore, hair related gene loci provided by the invention library is very comprehensive, accurate.
3. hair related gene polymorphism of the present invention site shares 52, had studied by Snpedia each more
The genotype in state property site considers the relationship between each genotype, and gives different scorings；After obtaining user's gene, pass through
The genotype of each gene loci of user is compared with gene loci library and carries out scoring calculating, you can learn that the hair-like of user
State.The assessment method of hair state is simple, after knowing potential hair problem, can carry out hair nursing ahead of time, prevention hair is asked
The appearance of topic.In addition, this law can also help the user that there are problems that hair to find the basic reason for causing hair problem, precisely
Nursing improves hair quality.
4. carrying out hair state evaluation using the relevant gene loci library of hair provided by the invention, only need to have user's
Genetic test result data, for gene tester, there is no limit have a wide range of application, convenient for promoting.
To sum up, technique of gene detection is used for the diagnosis of hair by the present invention, provides a set of body that simply and easily scores
System helps user accurately to find a root for human hair's problem from genic level, dissects hair problem, allow each
The hair care behavior of people can realize personalized customization with treating both manifestation and root cause of disease, with a definite target in view.
Specific embodiment
The present invention provides a kind of relevant gene loci libraries of hair, including SRD5A2 gene, 2-V89L gene, AR base
Cause, DHT gene, FGF-5 gene, MAPK gene, HIF gene, BCMO1 gene, SELL gene, DDB2 gene, IL-6 gene,
TNF-alpha gene, Edar gene, VDR gene, CYP450 gene, DAG gene, MCR1 gene, XRCC1 gene, ERCC2 base
Cause, KITLG gene, TYR gene, TYRP1 gene, OCA2 gene, Wnt gene and beta-catenin gene.
SRD5A2 gene, 2-V89L gene, AR gene, DHT gene, FGF-5 gene are related to hormone-type alopecia, SRD5A2
Gene polynorphisms site is rs523349, rs1691053 and rs700519, and 2-V89L gene polynorphisms site is
Rs1799941 and rs6259, AR gene polynorphisms site are rs722208 and rs1204038, DHT gene polynorphisms site
For rs1691053 and rs9287638, FGF-5 gene polynorphisms site is rs2981582 and rs13317.Pass through Snpedia
The relationship between each genotype is researched and analysed, gives the different genotype of polymorphic site different scorings, same polymorphic position
The same gene type of point contains there are many when scoring, is calculated with more high score.
SRD5A2 gene polynorphisms site rs523349 mutually should be 33 points, 16.5 points and 1 with the scoring of GG for CC, CG
Point, rs1691053 AA, AG mutually should be 33 points, 16.5 points and 1 point with the scoring of GG, and rs700519 is the scoring of TT CT CC
It mutually should be 33 points, 16.5 points and 1 point；Scoring, the higher activity for indicating 5 alpha-reductases is higher, and hormone-type alopecia is more serious.
2-V89L gene polynorphisms site rs1799941 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG,
Rs6259 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG, and the higher expression 3male hormone secretion level that scores is higher,
Hormone-type alopecia is more serious.
AR gene polynorphisms site rs722208 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG,
Rs1204038 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT, and the higher expression AR acceptor levels that score are higher, swash
Plain type alopecia is more serious.
DHT gene polynorphisms site rs1691053 mutually should be 50 points, 25 points and 1 point with the scoring of AA for GG, AG,
Rs9287638 mutually should be 50 points, 25 points and 1 point with the scoring of CC for AA, AC, and the higher expression DHT acceptor levels that score are higher, swash
Plain type alopecia is more serious.
FGF-5 gene polynorphisms site rs2981582 mutually should be 50 points, 25 points and 1 point with the scoring of CC for TT, CT,
Rs13317 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT, score higher expression FGF-5 gene expression dose more
Height, hormone-type alopecia are more serious.
MAPK gene and HIF gene are related to disorders of lipid metabolism, and BCMO1 gene is related to fat secretion, unconventionality expression
Alopecia seborrheica, hair can be caused to dry up, and short-tempered, hair is greasy, hair dries caused dandruff and the greasy caused head of hair
Bits.In addition, BCMO1 Overexpression, so that smegma increases, hair follicle easily blocks, influences natural on-off cycles of hair growth, hair occur
Sparse symptom.
MAPK gene polynorphisms site is rs13317, rs12701937 and rs56234898, HIF gene polynorphisms
Site is rs11549465 and rs2057482；BCMO1 gene polynorphisms site be rs7501331, rs119478057 and
rs12934922。
MAPK gene is related to disorders of lipid metabolism, and polymorphic site rs13317 mutually should be 50 with the scoring of CC for TT, CT
Point, 25 points and 1 point, rs12701937 CC, CT and the scoring of TT mutually should be 50 points, 25 points and 1 point, rs56234898 AA,
AG mutually should be 50 points, 25 points and 1 point with the scoring of GG, and the higher expression lipid-metabolism that scores is stronger.
HIF gene is related to disorders of lipid metabolism, and polymorphic site rs11549465 is that CC, CT mutually should be with the scoring of TT
50 points, 25 points and 1 point, rs2057482 CC, CT mutually should be 50 points, 25 points and 1 point with the scoring of TT, and score higher expression rouge
Matter metaboilic level is higher.
BCMO1 gene is related to fat secretion, and polymorphic site rs7501331 mutually should be 33 with the scoring of TT for CC, CT
Divide, 16.5 points and 1, rs119478057 AA, AT mutually should be 33 points, 16.5 points and 1 point with the scoring of TT, and rs12934922 is
TT, AT mutually should be 33 points, 16.5 points and 1 point with the scoring of AA, and the higher expression Lipid Secretion level that scores is higher, explanation
When rs7501331, rs119478057 and rs12934922 are respectively CC, AA and TT, overall score highest, fat secretion level is most
It is high.
SELL gene and DDB2 gene are related to bacterium neurological susceptibility, and unconventionality expression can cause folliculitis decalvans and inflammation to draw
The dandruff risen.SELL gene polynorphisms site is rs2229569 and rs4987310, and DDB2 gene polynorphisms site is
Rs747650 and rs7531806.
SELL gene polynorphisms site rs2229569 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG,
Rs4987310 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG, and higher expression of scoring is easier by bacterium sense
Dye.
DDB2 gene polynorphisms site rs747650 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG,
Rs7531806 mutually should be 25 points, 12.5 points and 1 point with the scoring of GG for AA, AG, and higher expression of scoring is easier by bacterium sense
Dye.
IL-6 gene, TNF-alpha gene are related to inflammatory factor secretion, unconventionality expression will lead to folliculitis decalvans,
Scalp sensitivity dandruff and scalp inflammation dandruff.IL-6 gene polynorphisms site be rs1474347, rs1548216 and
Rs1800795, TNF-alpha gene polynorphisms site are rs1800629 and rs361525.
IL-6 gene polynorphisms site rs1474347 mutually should be 20 points, 10 points and 1 point with the scoring of GG for TT, GT,
Rs1548216 mutually should be 20 points, 10 points and 1 point with the scoring of CC for GG, GC, and the scoring of rs1800795 GG, GC and CC are corresponding
It is 20 points, 10 points and 1 point, the higher expression inflammatory factor secretion level that scores is higher.
TNF-alpha gene polynorphisms site rs1800629 mutually should be 20 points, 10 points and 1 with the scoring of GG for AA, AG
Point, rs361525 GG, AG mutually should be 20 points, 10 points and 1 point with the scoring of AA, and score higher expression inflammatory factor secretion level
It is higher.
Edar gene and VDR gene are bad related to hair follicle development, and Edar gene influences hair follicle growth signal path, VDR
Gene expression causes vitamin D receptor insufficient, eventually leads to hair sparse.Edar gene polynorphisms site is rs3827760
And rs121908450, VDR gene polynorphisms site are rs1544410 and rs2228570.
Edar gene polynorphisms site is that rs3827760 is that CC, CT with the scoring of TT mutually should be 50 points, 25 points and 1 point,
Rs121908450 mutually should be 50 points, 25 points and 1 point with the scoring of AA for GG, AG, and the higher expression hair follicle growth signal of scoring point is logical
Roadbed is more serious because inactivating, and hair follicle development is bad.
VDR gene polynorphisms site rs1544410 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG,
Rs2228570 mutually should be 50 points, 25 points and 1 point with the scoring of CC for TT, CT, score higher expression vitamin D receptor level more
Low, hair follicle development is bad.
CYP450 gene is easily destroyed related to hair follicle, will lead to hair sparse.CYP450 gene polynorphisms site is
Rs11636419 and rs17861162.
CYP450 gene polynorphisms site rs11636419 mutually should be 50 points, 25 points and 1 with the scoring of AA for CC, AG
Point, rs17861162 CC, CG mutually should be 50 points, 25 points and 1 point with the scoring of GG, and the higher expression hair follicle that scores protects aromatase enzyme
Horizontal lower, hair follicle is easily destroyed.
DAG gene is related to keratin secretion, and keratin hyposecretion leads to hair sparse, and hair is nonelastic, fragile.
DAG gene polynorphisms site is rs4625 and rs12583, rs4625 TT, CT and the scoring of CC mutually should be 50 points, 25 points and
1 point, rs12583 TT, GT mutually should be 50 points, 25 points and 1 point with the scoring of GG, score higher expression keratin secretion level more
It is low.
MCR1 gene, XRCC1 gene and ERCC2 gene are related to anti-uv-ray, and it is crisp that unconventionality expression will lead to hair
It is weak easily broken；MCR1 gene is also related to melanosome synthesis, and unconventionality expression will lead to congenital white hair.MCR1 gene polynorphisms position
Point is rs2228479 and rs1805008, and XRCC1 gene polynorphisms site is rs25487 and rs1799782, ERCC2 gene
Polymorphic site be rs13181.
When MCR1 gene evaluates anti-uv-ray, polymorphic site rs2228479 is that AA, AG mutually should be with the scoring of GG
20 points, 10 points and 1 point, rs1805008 TT, CT mutually should be 20 points, 10 points and 1 point with the scoring of CC, and higher expression of scoring is anti-
UV resistance is poorer, and when illustrating that rs2228479 and rs1805008 are respectively AA and TT, 40 points of scoring are maximum value, uvioresistant
Ability is poor, and hair fragility is easily broken；When evaluating melanosome synthesis, polymorphic site rs2228479 is that the scoring of AA, AG and GG are corresponding
It is 1 point, 25 points and 50 points, rs1805008 TT, CT mutually should be 1 point, 25 points and 50 points with the scoring of CC, higher expression of scoring
Melanosome synthesis is horizontal lower；When illustrating that rs2228479 and rs1805008 are respectively GG and CC, scoring is 100 points, melanosome
Synthesis is insufficient, congenital white hair occurs.
XRCC1 gene polynorphisms site rs25487 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG,
Rs1799782 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT, and the higher expression anti-uv-ray that scores is poorer.
ERCC2 gene polynorphisms site rs13181 mutually should be 20 points, 10 points and 1 point with the scoring of TT for GG, GT, comment
Divide higher expression anti-uv-ray poorer.
KITLG gene, TYR gene, TYRP1 gene and OCA2 gene are related to congenital white hair, KITLG gene it is polymorphic
Property site be rs755383 and rs794726675, TYR gene polynorphisms site be rs1393350 and rs1126809, TYRP1
Gene polynorphisms site is rs2733832, and OCA2 gene polynorphisms site is rs1800401 and rs1800414.
KITLG gene polynorphisms site rs755383 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT,
Rs794726675 mutually should be 50 points, 25 points and 1 point with the scoring of TT for AA, AT, and the higher expression melanocyte proliferation that scores is horizontal
Lower, congenital white hair is more serious.
TYR gene polynorphisms site rs1393350 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG,
Rs1126809 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG, and the higher expression tyrosinase activity that scores is lower,
Congenital white hair is more serious.
TYRP1 gene polynorphisms site rs2733832 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT,
Scoring, higher expression tyrosinase acceptor levels are lower, and congenital white hair is more serious.
OCA2 gene polynorphisms site rs1800401 mutually should be 20 points, 10 points and 1 point with the scoring of GG for AA, AG,
Rs1800414 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT, and the higher expression tyrosinase activity that scores is lower,
Congenital white hair is more serious.
Wnt gene and beta-catenin gene are related to Aging white hair, and Wnt gene polynorphisms site is rs7349332
And rs7775, the polymorphic site of beta-catenin gene are rs524952 and rs2327832.
Wnt gene polynorphisms site rs7349332 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT,
Rs7775 mutually should be 50 points, 25 points and 1 point with the scoring of GG for CC, CG, the higher gene expression for indicating Wnt signal path of scoring
Horizontal lower, Aging white hair is more serious.
The polymorphic site rs524952 of beta-catenin gene mutually should be 50 points, 25 points and 1 with the scoring of TT for AA, AT
Point, rs2327832 AA, AG mutually should be 50 points, 25 points and 1 point with the scoring of GG, higher expression melanocyte mother cell differentiation of scoring
Suppressed degree is stronger, and Aging white hair is more serious.
This research is based on the genetic test for determining hair state GAP-associated protein GAP, and testing result reflects protein level
Difference further analyzes GAP-associated protein GAP activity, clearly influences the determinant of hair state, track corresponding gene loci.
The standards of grading of gene loci library above-mentioned and each polymorphic site can be applied to the hair state of individual
Evaluation.The method of hair state evaluation the following steps are included:
S1. the gene of user is obtained；
S2. the gene in gene gene loci relevant with hair library that step S1 is obtained is compared, determines gene
The genotype of polymorphic site calculates scoring；The calculation method of scoring is that the scoring of each polymorphic site of each gene is folded
Add, if some genotype of certain polymorphic site there are several scorings, takes best result；
S3. according to the hair state for determining user that scores.
In step S1, the gene of user is obtained using high throughput sequencing technologies SOLID, experimental procedure are as follows: (1) adopt
Collect the saliva sample of user；(2) Ambion is usedRNA isolation kit extracts the total serum IgE in sample, sieve
The mRNA in sample is selected, which is RIN >=7 by Agilent2100BioAnalyzer testing result；(3) in PCR instrument
On, sample mRNA is purified and fragmentation using SOLiD whole transcriptome expression RNA isolation kit, and
Recycle digestion products, i.e. SOLiD RNA；(4) SOLiD whole transcriptome expression RNA isolation kit is used,
SOLiD RNA is connect with special connector, reverse transcription and PCR amplification is then carried out, obtains PCR product；(5) PCR product is pre-
Processing, obtains sample to be tested, and then upper machine sequencing, obtains the gene of user.The concrete operations of each step referring to each kit or
The operation instruction of person's test equipment carries out, and belongs to conventional genetic test means.
In step S1, the method for obtaining user's gene, which can also be, carries out genetic test using other any methods,
Obtained genetic test report, user only need to upload genetic test report online, easily and fast.
The scoring of said gene site library and each polymorphic site is set, and is the basis for carrying out hair state evaluation.If certain
The expression of polymorphic site is related to a variety of hair problems, then is calculated using high score.
Unless otherwise defined, all technical and scientific terms used herein and the skill for belonging to technical field of the invention
The normally understood meaning of art personnel is identical.To be intended merely to description specific for used term in the description of the invention herein
The purpose of embodiment, it is not intended that in the limitation present invention.
The present invention is illustrated with specific example below, the basis that each embodiment is carried out is first to construct above-mentioned hair phase
The gene loci library of pass, and set the scoring of each polymorphic site gene expression.
Embodiment 1
Totally 5 users provide genetic test report (not providing other information), respectively provide its gene with the present invention
Gene loci library be compared, and calculate scoring, as a result are as follows:
User's 1:SRD5A2 gene polynorphisms site rs523349 be CC, rs1691053 AA, rs700519 TT,
The scoring of SRD5A2 gene is 99 points；2-V89L gene polynorphisms site rs1799941 is AA, rs6259 AA, 2-V89L
The scoring of gene is 100 points；AR gene polynorphisms site rs722208 is AA, and the scoring of rs1204038 CC, AR gene is
100 points；DHT gene polynorphisms site rs1691053 is GG, and the scoring of rs9287638 AA, DHT gene is 100 points；
FGF-5 gene polynorphisms site rs2981582 is TT, and the scoring of rs13317 CC, FGF-5 gene is 100 points.Other bases
Normal because expressing, thus inferring the user, there are the caducous problems of hair, and belong to hormone-type alopecia, and basic reason is
5 alpha-reductase hyperactivities, 3male hormone secretion level are excessively high, AR acceptor levels are excessively high, DHT acceptor levels are excessively high and FGF-5 base
Because of overexpression.
User's 2:SRD5A2 gene polynorphisms site rs523349 be CC, rs1691053 AA, rs700519 TT,
The scoring of SRD5A2 gene is 99 points；2-V89L gene polynorphisms site rs1799941 is AA, rs6259 AG, 2-V89L
The scoring of gene is 75 points；AR gene polynorphisms site rs722208 is AG, and the scoring of rs1204038 CC, AR gene is
75 points；DHT gene polynorphisms site rs1691053 is AG, and the scoring of rs9287638 AC, DHT gene is 50 points；FGF-5
Gene polynorphisms site rs2981582 is TT, and the scoring of rs13317 CC, FGF-5 gene is 100 points.Other gene expressions
Normally, thus inferring the user, there are the caducous problems of hair, and belong to hormone-type alopecia.
User's 3:SRD5A2 gene polynorphisms site rs523349 be CC, rs1691053 AA, rs700519 TT,
The scoring of SRD5A2 gene is 99 points；2-V89L gene polynorphisms site rs1799941 is AA, rs6259 AG, 2-V89L
The scoring of gene is 75 points；AR gene polynorphisms site rs722208 is AG, and the scoring of rs1204038 CC, AR gene is
75 points；DHT gene polynorphisms site rs1691053 is GG, and the scoring of rs9287638 AA, DHT gene is 100 points；FGF-
5 gene polynorphisms site rs2981582 are TT, and the scoring of rs13317 CC, FGF-5 gene is 100 points.Other gene tables
Up to normal, thus inferring the user, there are the caducous problems of hair, and belong to hormone-type alopecia.
User's 4:SRD5A2 gene polynorphisms site rs523349 be CC, rs1691053 AA, rs700519 TT,
The scoring of SRD5A2 gene is 99 points；2-V89L gene polynorphisms site rs1799941 is AA, rs6259 AA, 2-V89L
The scoring of gene is 100 points；AR gene polynorphisms site rs722208 is AA, and the scoring of rs1204038 CC, AR gene is
100 points；DHT gene polynorphisms site rs1691053 is AG, and the scoring of rs9287638 AC, DHT gene is 50 points；FGF-
5 gene polynorphisms site rs2981582 are TT, and the scoring of rs13317 CT, FGF-5 gene is 75 points.Other gene expressions
Normally, thus inferring the user, there are the caducous problems of hair, and belong to hormone-type alopecia.
User's 5:SRD5A2 gene polynorphisms site rs523349 be CC, rs1691053 AA, rs700519 TT,
The scoring of SRD5A2 gene is 99 points；2-V89L gene polynorphisms site rs1799941 is AA, rs6259 AA, 2-V89L
The scoring of gene is 100 points；AR gene polynorphisms site rs722208 is AG, and the scoring of rs1204038 CC, AR gene is
75 points；DHT gene polynorphisms site rs1691053 is GG, and the scoring of rs9287638 AA, DHT gene is 100 points；FGF-
5 gene polynorphisms site rs2981582 are TT, and the scoring of rs13317 CC, FGF-5 gene is 100 points.Other gene tables
Up to normal, thus inferring the user, there are the caducous problems of hair, and belong to hormone-type alopecia.
There is easy alopecia in each user, and the problem of, dry etc greasy without hair；Using gene loci library
The result for evaluating the hair state of 5 users is easy alopecia, be consistent with truth, it was demonstrated that gene loci library it is accurate
Property.On this basis, for hormone-type alopecia the reason of, can be prevented hair loss well using corresponding hair-care agent.
Embodiment 2
Totally 3 users provide genetic test report (not providing other information), respectively provide its gene with the present invention
Gene loci library be compared, and calculate scoring, as a result are as follows:
User's 1:SRD5A2 gene polynorphisms site rs523349 be CC, rs1691053 AA, rs700519 TT,
The scoring of SRD5A2 gene is 99 points；AR gene polynorphisms site rs722208 is AA, rs1204038 CC, AR gene
Scoring is 100 points；FGF-5 gene polynorphisms site rs2981582 is TT, and the scoring of rs13317 CC, FGF-5 gene is
100 points；MAPK gene polynorphisms site is that rs13317 is TT, rs12701937 CC, rs56234898 AA, MAPK base
The scoring of cause is 150 points, indicates that lipid-metabolism is vigorous；HIF gene polynorphisms site rs11549465 is CC, rs2057482
For CC, the scoring of HIF gene is 100 points, indicates that lipid-metabolism is vigorous；BCMO1 gene polynorphisms site rs7501331 is
The scoring of CC, rs119478057 AA, rs12934922 TT, BCMO1 gene is 99 points, indicates that lipid-metabolism is vigorous.Other
Gene expression is normal, thus infers that the user has that hair is easy to fall off, greasy and dandruff, and alopecia is by hormone point
It secretes and Lipid Secretion excessively causes, also cause that hair is greasy and dandruff while Lipid Secretion is vigorous；And the head of the user
Hair grows sparse in itself.After being linked up with user, it was confirmed that hair evaluation result is completely correct, and the hair of user is greasy, dandruff,
Also alopecia.Hair state evaluation result, while the reason of causing each hair problem is given, hair conditioning is verified, is changed well
It has been apt to hair quality.
User's 2:MAPK gene polynorphisms site is that rs13317 is CC, and rs12701937 TT, rs56234898 are
The scoring of GG, MAPK gene is 3 points, indicates that lipid-metabolism level is very low；HIF gene polynorphisms site rs11549465 is
The scoring of TT, rs2057482 TT, HIF gene is 2 points, indicates that lipid-metabolism level is very low；BCMO1 gene polynorphisms position
Point rs7501331 is TT, and the scoring of rs119478057 TT, rs12934922 AA, BCMO1 gene is 3 points, indicates lipid
Metaboilic level is very low.Other gene expressions are normal, thus infer that the user dries up short-tempered and dries up short-tempered cause there are hair
Dandruff problem.It is consistent with the truth of user, recommended user, can be very using the hair products of enhancing scalp lipid-metabolism
Good improvement hair quality does not occur short-tempered and dandruff problem.
User's 3:SELL gene polynorphisms site rs2229569 is GG, the scoring of rs4987310 GG, SELL gene
It is 50 points；DDB2 gene polynorphisms site rs747650 is GG, and the scoring of rs7531806 AA, DDB2 gene is 50 points.Its
His gene expression is normal, infers that the user has the slight epifolliculitis due to caused by bacterium infection, with dandruff.With user's
Truth is consistent, it is recommended to use the susceptible care regimen of bacterium can improve scalp inflammation situation well, dispel dandruff.
Embodiment 3
The gene that user is obtained using high throughput sequencing technologies, is then compared with gene loci library provided by the invention
It is right, and scoring is calculated, as a result are as follows:
SELL gene polynorphisms site rs2229569 is GG, and the scoring of rs4987310 GG, SELL gene is 50 points；
DDB2 gene polynorphisms site rs747650 is GG, and the scoring of rs7531806 AA, DDB2 gene is 50 points；IL-6 gene
Polymorphic site rs1474347 be TT, the scoring of rs1548216 GG, rs1800795 GG, IL-6 gene be 60 points；
TNF-alpha gene polynorphisms site rs1800629 is AA, and the scoring of rs361525 GG, TNF-alpha gene is 40
Point.Other gene expressions are normal, and inferring the user, there are epifolliculitis, with dandruff.It is consistent with the truth of user, recommends
Using inflammation care regimen, scalp inflammation situation can be improved well, dispel dandruff.
Embodiment 4
The gene that user is obtained using high throughput sequencing technologies, is then compared with gene loci library provided by the invention
It is right, and scoring is calculated, as a result are as follows:
Edar gene polynorphisms site is that rs3827760 is CC, and the scoring of rs121908450 GG, Edar gene is
100 points；VDR gene polynorphisms site rs1544410 is AA, and the scoring of rs2228570 TT, VDR gene is 100 points；
CYP450 gene polynorphisms site rs11636419 is AG, and the scoring of rs17861162 CC, CYP450 gene is 75 points.Its
His gene expression is normal, infers that the user has that hair sparse, basic reason are that hair follicle development is bad, easy with hair follicle
It is destroyed.The problem of user is implicitly present in hair sparse, it is recommended to use the care regimen for promoting hair follicle development, protecting hair follicle,
Hair amount can be effectively increased.
Embodiment 5
The gene that user is obtained using high throughput sequencing technologies, is then compared with gene loci library provided by the invention
It is right, and scoring is calculated, as a result are as follows:
AR gene polynorphisms site rs722208 is AA, and the scoring of rs1204038 CC, AR gene is 100 points；FGF-
5 gene polynorphisms site rs2981582 are TT, and the scoring of rs13317 CC, FGF-5 gene is 100 points；DAG gene it is more
State property site rs4625 is TT, and the scoring of rs12583 TT, DAG gene is 100 points.Other gene expressions are normal, infer the use
The problem of there are hair sparses, nonelastic, fragile easily broken, also easy alopecia at family.It is consistent with the truth of user, hair is dilute
Thin, nonelastic, fragile is due to keratin hyposecretion, and alopecia is that AR acceptor levels are excessively high, FGF-5 Overexpression, shadow
Caused by ringing hormone secretion, it is recommended to use targetedly care regimen can effectively improve hair quality.
Embodiment 6
The gene that user is obtained using high throughput sequencing technologies, is then compared with gene loci library provided by the invention
It is right, and scoring is calculated, as a result are as follows:
MCR1 gene polymorphism sites rs2228479 is AA, and the scoring of rs1805008 TT, MCR1 gene is 40 points；
XRCC1 gene polynorphisms site rs25487 is GG, and the scoring of rs1799782 TT, XRCC1 gene is 40 points；ERCC2 base
The polymorphic site rs13181 of cause is GG, is scored 20 points.Other gene expressions are normal, infer that the hair fragility of the user is easily broken,
It is consistent with truth.The fragile easily broken reason of hair is that anti-uv-ray is poor, therefore uses antiultraviolet spraying or anti-
The care regimen of solarization is effectively improved hair quality, enhances hair toughness.
Embodiment 7
The gene of 3 users is obtained using high throughput sequencing technologies, is then carried out with gene loci library provided by the invention
It compares, and calculates scoring, as a result are as follows:
User 1:MCR1 gene polymorphism sites rs2228479 is GG, and the scoring of rs1805008 CC, MCR1 gene is
100 points.Other gene expressions are normal, infer that the congenital white hair of the user is more, are consistent with truth, basic reason
It is synthesized for melanosome insufficient, it is recommended to use the hair products for promoting melanosome synthesis can effectively reduce white hair amount.
User's 2:KITLG gene polynorphisms site rs755383 is CC, and rs794726675 AA, KITLG gene is commented
It is divided into 100 points；TYR gene polynorphisms site rs1393350 is GG, and the scoring of rs1126809 GG, TYR gene is 40 points；
TYRP1 gene polynorphisms site rs2733832 is TT, is scored 20 points；OCA2 gene polynorphisms site rs1800401 is
The scoring of AA, rs1800414 TT, OCA2 gene is 40 points.Other gene expressions are normal, thus infer that there are congenital by the user
Property white hair, is consistent with truth.Scoring highest is KITLG gene, indicates that the most important reason of congenital white hair is black
Plain cell Proliferation is few；Simultaneously it is also contemplated that the low factor of tyrosinase activity can be effectively reduced white using corresponding hair products
Hair amount.
User's 3:Wnt gene polynorphisms site rs7349332 is CC, and the scoring of rs7775 CC, Wnt gene is 100
Point；The polymorphic site rs524952 of beta-catenin gene is AA, and the scoring of rs2327832 AG, beta-catenin gene are
75 points.Other gene expressions are normal, thus infer that the user there are Aging white hair, is consistent with truth.Score highest
It is Wnt gene, is secondly beta-catenin gene, it is related to hair follicle aging, therefore delay hair follicle aging using corresponding
Conditioner, can effectively slow down, reduce hair and bleach.
To sum up, gene loci library provided by the invention is comprehensive, and the method for evaluating user's hair state using it is simple, as a result
Accurately, the root that user's hair problem can be found from the level of gene provides targetedly hair nursing scheme, realizes personalized
Customization.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of relevant gene loci library of hair, which is characterized in that including SRD5A2 gene, 2-V89L gene, AR gene,
DHT gene, FGF-5 gene, MAPK gene, HIF gene, BCMO1 gene, SELL gene, DDB2 gene, IL-6 gene, TNF-
Alpha gene, Edar gene, VDR gene, CYP450 gene, DAG gene, MCR1 gene, XRCC1 gene, ERCC2 gene,
KITLG gene, TYR gene, TYRP1 gene, OCA2 gene, Wnt gene and beta-catenin gene.
2. the relevant gene loci library of hair according to claim 1, which is characterized in that the SRD5A2 gene, 2-
V89L gene, AR gene, DHT gene, FGF-5 gene are related to hormone-type alopecia, the MAPK gene and HIF gene and lipid
Metabolic disorder is related, and the BCMO1 gene is related to fat secretion, the SELL gene and DDB2 gene and bacterium neurological susceptibility phase
It closes, IL-6 gene, TNF-alpha gene are related to inflammatory factor secretion, Edar gene and VDR gene and the bad phase of hair follicle development
Close, CYP450 gene is easily destroyed related to hair follicle, and DAG gene is related to keratin secretion, MCR1 gene, XRCC1 gene and
ERCC2 gene is related to anti-uv-ray, KITLG gene, TYR gene, TYRP1 gene and OCA2 gene and congenital white hair
Correlation, Wnt gene and beta-catenin gene are related to Aging white hair.
3. the relevant gene loci library of hair according to claim 1, which is characterized in that the SRD5A2 gene it is polymorphic
Property site be rs523349, rs1691053 and rs700519,2-V89L gene polynorphisms site be rs1799941 and
Rs6259, AR gene polynorphisms site are rs722208 and rs1204038, and DHT gene polynorphisms site is rs1691053
And rs9287638, FGF-5 gene polynorphisms site are rs2981582 and rs13317.
4. the relevant gene loci library of hair according to claim 2, which is characterized in that the MAPK gene polynorphisms
Site be rs13317, rs12701937 and rs56234898, HIF gene polynorphisms site be rs11549465 and
rs2057482；BCMO1 gene polynorphisms site is rs7501331, rs119478057 and rs12934922.
5. the relevant gene loci library of hair according to claim 2, which is characterized in that the SELL gene polynorphisms
Site is rs2229569 and rs4987310, and DDB2 gene polynorphisms site is rs747650 and rs7531806, the IL-6
Gene polynorphisms site is rs1474347, rs1548216 and rs1800795, and TNF-alpha gene polynorphisms site is
Rs1800629 and rs361525, Edar gene polynorphisms site are rs3827760 and rs121908450, VDR gene
Polymorphic site be rs1544410 and rs2228570, CYP450 gene polynorphisms site be rs11636419 and
Rs17861162, DAG gene polynorphisms site are rs4625 and rs12583, and the MCR1 gene is also closed with melanosome
At correlation, polymorphic site is rs2228479 and rs1805008, XRCC1 gene polynorphisms site be rs25487 and
Rs1799782, ERCC2 gene polynorphisms site are rs13181, and KITLG gene polynorphisms site is rs755383
And rs794726675, TYR gene polynorphisms site are rs1393350 and rs1126809, TYRP1 gene polynorphisms site
For rs2733832, OCA2 gene polynorphisms site is rs1800401 and rs1800414, Wnt gene polynorphisms position
Point is rs7349332 and rs7775, and the polymorphic site of beta-catenin gene is rs524952 and rs2327832.
6. the relevant gene loci library of hair according to claim 5, which is characterized in that MCR1 gene polynorphisms site
When rs2228479 and rs1805008 are respectively AA and TT, anti-uv-ray is poor, and hair fragility is easily broken；Rs2228479 and
When rs1805008 is respectively GG and CC, melanosome synthesis is insufficient, congenital white hair occurs.
7. application of the relevant gene loci library of hair described in claim 3 or 4 in hair state evaluation, feature exist
In, comprising the following steps:
S1. the gene of user is obtained；
S2. the gene in gene gene loci relevant with hair library that step S1 is obtained is compared, determines gene polymorphic
Property site genotype, calculate scoring；
S3. according to the hair state for determining user that scores.
8. application of the relevant gene loci library of hair according to claim 7 in hair state evaluation, feature exist
In the method for calculating scoring described in step S2 is the scoring superposition of each polymorphic site of each gene, wherein each base
Because of the standards of grading of polymorphic site are as follows:
SRD5A2 gene polynorphisms site rs523349 mutually should be 33 points, 16.5 points and 1 point with the scoring of GG for CC, CG,
Rs1691053 mutually should be 33 points, 16.5 points and 1 point with the scoring of GG for AA, AG, and rs700519 is that the scoring of TT CT CC is corresponding
It is 33 points, 16.5 points and 1 point, scoring, the higher activity for indicating 5 alpha-reductases is higher, and hormone-type alopecia is more serious；
2-V89L gene polynorphisms site rs1799941 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG,
Rs6259 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG, and the higher expression 3male hormone secretion level that scores is higher,
Hormone-type alopecia is more serious；
AR gene polynorphisms site rs722208 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG, rs1204038
It mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT, scoring, higher expression AR acceptor levels are higher, and hormone-type alopecia is got over
Seriously；
DHT gene polynorphisms site rs1691053 mutually should be 50 points, 25 points and 1 point with the scoring of AA for GG, AG,
Rs9287638 mutually should be 50 points, 25 points and 1 point with the scoring of CC for AA, AC, and the higher expression DHT acceptor levels that score are higher, swash
Plain type alopecia is more serious；
FGF-5 gene polynorphisms site rs2981582 mutually should be 50 points, 25 points and 1 point with the scoring of CC for TT, CT,
Rs13317 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT, score higher expression FGF-5 gene expression dose more
Height, hormone-type alopecia are more serious；
MAPK gene is related to disorders of lipid metabolism, polymorphic site rs13317 be TT, CT and the scoring of CC mutually should be 50 points,
25 points and 1 point, rs12701937 CC, CT and the scoring of TT mutually should be 50 points, 25 points and 1 point, rs56234898 AA, AG and
The scoring of GG mutually should be 50 points, 25 points and 1 point, and the higher expression lipid-metabolism that scores is stronger；
HIF gene is related to disorders of lipid metabolism, and polymorphic site rs11549465 mutually should be 50 with the scoring of TT for CC, CT
Divide, 25 points and 1 point, rs2057482 CC, CT mutually should be 50 points, 25 points and 1 point with the scoring of TT, and score higher expression lipid
Metaboilic level is higher；
BCMO1 gene is related to fat secretion, polymorphic site rs7501331 be CC, CT and the scoring of TT mutually should be 33 points,
16.5 points and 1, rs119478057 AA, AT and the scoring of TT mutually should be 33 points, 16.5 points and 1 point, rs12934922 TT,
AT mutually should be 33 points, 16.5 points and 1 point with the scoring of AA, and the higher expression fat secretion level that scores is higher.
9. application of the relevant gene loci library of hair described in claim 5 in hair state evaluation, which is characterized in that packet
Include following steps:
S1. the gene of user is obtained；
S2. the gene in gene gene loci relevant with hair library that step S1 is obtained is compared, determines gene polymorphic
Property site genotype, calculate scoring；
S3. according to the hair state for determining user that scores, the highest gene that scores is the determinant of hair state；
Wherein, the method for calculating scoring is the scoring superposition of each polymorphic site of each gene, same polymorphic site
Same gene type contains there are many when scoring, is calculated with more high score.
10. application of the relevant gene loci library of hair according to claim 9 in hair state evaluation, feature exist
In the standards of grading of each gene polymorphism sites are as follows:
SELL gene polynorphisms site rs2229569 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG,
Rs4987310 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG, and higher expression of scoring is easier by bacterium sense
Dye；
DDB2 gene polynorphisms site rs747650 mutually should be 25 points, 12.5 points and 1 point with the scoring of AA for GG, AG,
Rs7531806 mutually should be 25 points, 12.5 points and 1 point with the scoring of GG for AA, AG, and higher expression of scoring is easier by bacterium sense
Dye；
IL-6 gene polynorphisms site rs1474347 mutually should be 20 points, 10 points and 1 point with the scoring of GG for TT, GT,
Rs1548216 mutually should be 20 points, 10 points and 1 point with the scoring of CC for GG, GC, and the scoring of rs1800795 GG, GC and CC are corresponding
It is 20 points, 10 points and 1 point, the higher expression inflammatory factor secretion level that scores is higher；
TNF-alpha gene polynorphisms site rs1800629 mutually should be 20 points, 10 points and 1 point with the scoring of GG for AA, AG,
Rs361525 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG, score higher expression inflammatory factor secretion level more
It is high；
Edar gene polynorphisms site is that rs3827760 is that CC, CT with the scoring of TT mutually should be 50 points, 25 points and 1 point,
Rs121908450 mutually should be 50 points, 25 points and 1 point with the scoring of AA for GG, AG, and score higher expression hair follicle growth signal path
Gene inactivation is more serious, and hair follicle development is bad；
VDR gene polynorphisms site rs1544410 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG,
Rs2228570 mutually should be 50 points, 25 points and 1 point with the scoring of CC for TT, CT, score higher expression vitamin D receptor level more
Low, hair follicle development is bad；
CYP450 gene polynorphisms site rs11636419 mutually should be 50 points, 25 points and 1 point with the scoring of AA for CC, AG,
Rs17861162 mutually should be 50 points, 25 points and 1 point with the scoring of GG for CC, CG, and the higher expression hair follicle that scores protects aromatase enzyme water
Flat lower, hair follicle is easily destroyed；
DAG gene polynorphisms site rs4625 mutually should be 50 points, 25 points and 1 point with the scoring of CC for TT, CT, and rs12583 is
TT, GT mutually should be 50 points, 25 points and 1 point with the scoring of GG, and the higher expression keratin secretion level that scores is lower；
When MCR1 gene evaluates anti-uv-ray, polymorphic site rs2228479 mutually should be 20 with the scoring of GG for AA, AG
Divide, 10 points and 1 point, rs1805008 TT, CT mutually should be 20 points, 10 points and 1 point with the scoring of CC, and score the higher anti-purple of expression
Outside line ability is poorer；When evaluating melanosome synthesis, polymorphic site rs2228479 be AA, AG and the scoring of GG mutually should be 1 point,
25 points and 50 points, rs1805008 TT, CT mutually should be 1 point, 25 points and 50 points with the scoring of CC, and score higher expression melanosome
Synthesis is horizontal lower, congenital white hair occurs；
XRCC1 gene polynorphisms site rs25487 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG,
Rs1799782 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT, and the higher expression anti-uv-ray that scores is poorer；
ERCC2 gene polynorphisms site rs13181 mutually should be 20 points, 10 points and 1 point with the scoring of TT for GG, GT, and scoring is got over
Height indicates that anti-uv-ray is poorer；
KITLG gene polynorphisms site rs755383 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT,
Rs794726675 mutually should be 50 points, 25 points and 1 point with the scoring of TT for AA, AT, and the higher expression melanocyte proliferation that scores is horizontal
Lower, congenital white hair is more serious；
TYR gene polynorphisms site rs1393350 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG,
Rs1126809 mutually should be 20 points, 10 points and 1 point with the scoring of AA for GG, AG, and the higher expression tyrosinase activity that scores is lower,
Congenital white hair is more serious；
TYRP1 gene polynorphisms site rs2733832 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT, scoring
Higher expression tyrosinase acceptor levels are lower, and congenital white hair is more serious；
OCA2 gene polynorphisms site rs1800401 mutually should be 20 points, 10 points and 1 point with the scoring of GG for AA, AG,
Rs1800414 mutually should be 20 points, 10 points and 1 point with the scoring of CC for TT, CT, and the higher expression tyrosinase activity that scores is lower,
Congenital white hair is more serious；
Wnt gene polynorphisms site rs7349332 mutually should be 50 points, 25 points and 1 point with the scoring of TT for CC, CT, rs7775
It mutually should be 50 points, 25 points and 1 point with the scoring of GG for CC, CG, score the higher gene expression dose for indicating Wnt signal path more
Low, Aging white hair is more serious；
The polymorphic site rs524952 of beta-catenin gene mutually should be 50 points, 25 points and 1 point with the scoring of TT for AA, AT,
Rs2327832 mutually should be 50 points, 25 points and 1 point with the scoring of GG for AA, AG, and higher expression melanocyte mother cell differentiation of scoring is suppressed
The degree of system is stronger, and Aging white hair is more serious.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2556178A1 (en) * | 2004-02-13 | 2005-09-01 | Tony N. Frudakis | Methods and compositions for inferring eye color and hair color |
| CN101545005A (en) * | 2008-03-27 | 2009-09-30 | 李文正纳米研究院 | Method for auxiliarily determining warfarin dosage for Chinese Han patients by utilizing single nucleotide polymorphism analysis and biological chip |
| CN106937957A (en) * | 2017-04-21 | 2017-07-11 | 北京越为科技有限公司 | A kind of application method for being used to treat the formula, shampoo and the formula of androgenetic alopecia |
| CN107400709A (en) * | 2017-07-17 | 2017-11-28 | 陈梦麟 | LincRNATUNAR is applied in regulation cerebral nervous system development |
| CN108472510A (en) * | 2015-07-30 | 2018-08-31 | 宾夕法尼亚大学理事会 | The single nucleotide polymorphism allele of the people's DP-2 genes for the neurological susceptibility that hair growth is inhibited for detecting PGD2 |
-
2018
- 2018-12-14 CN CN201811535278.3A patent/CN109355401A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2556178A1 (en) * | 2004-02-13 | 2005-09-01 | Tony N. Frudakis | Methods and compositions for inferring eye color and hair color |
| CN101545005A (en) * | 2008-03-27 | 2009-09-30 | 李文正纳米研究院 | Method for auxiliarily determining warfarin dosage for Chinese Han patients by utilizing single nucleotide polymorphism analysis and biological chip |
| CN108472510A (en) * | 2015-07-30 | 2018-08-31 | 宾夕法尼亚大学理事会 | The single nucleotide polymorphism allele of the people's DP-2 genes for the neurological susceptibility that hair growth is inhibited for detecting PGD2 |
| CN106937957A (en) * | 2017-04-21 | 2017-07-11 | 北京越为科技有限公司 | A kind of application method for being used to treat the formula, shampoo and the formula of androgenetic alopecia |
| CN107400709A (en) * | 2017-07-17 | 2017-11-28 | 陈梦麟 | LincRNATUNAR is applied in regulation cerebral nervous system development |
Non-Patent Citations (5)
| Title |
|---|
| KAUSTUBH ADHIKARI等: ""A genome-wide association scan in admixed Latin Americans identifies loci influencing facial and scalp hair features"", 《NAT COMMUN.》 * |
| PATRICK SULEM等: ""Genetic determinants of hair, eye and skin pigmentation in Europeans"", 《NATURE GENETICS》 * |
| RUI LI等: ""Six novel susceptibility Loci for early-onset androgenetic alopecia and their unexpected association with common diseases"", 《PLOS GENET.》 * |
| 罗云波主编: "《食品生物技术导论》", 31 August 2016, 北京：中国农业大学出版社 * |
| 黄颖之: ""遗传性毛发疾病的致病突变研究"", 《万方学位论文》 * |
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