CN109355356A - A kind of DNA biosensor and its method applied to measurement DNA - Google Patents
A kind of DNA biosensor and its method applied to measurement DNA Download PDFInfo
- Publication number
- CN109355356A CN109355356A CN201811239868.1A CN201811239868A CN109355356A CN 109355356 A CN109355356 A CN 109355356A CN 201811239868 A CN201811239868 A CN 201811239868A CN 109355356 A CN109355356 A CN 109355356A
- Authority
- CN
- China
- Prior art keywords
- dna
- electrode
- silver nanowires
- pdms
- biosensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
The present invention relates to a kind of DNA biosensor and its applied to the method for measurement DNA.Key point of the invention is that substrate PDMS is the flexible characteristic that flexible material determines electrode, silver nanowires has lower resistance, determine the excellent conductive performance of electrode, nanogold particle close-packed arrays in silver nanowires, biggish surface area is provided for fixation of the sulfydryl single stranded DNA on gold, good electric conductivity and higher active surface area are to prepare the guarantee of highly sensitive electrochemical sensor.The present invention prepares equally distributed silver nanowires conductive layer using the method for drop coating, then the method for electrochemical deposition is used to deposit nanogold particle in silver nanowires, its maximum advantage be it is simple and easy, do not need synthesis nanogold, it solves the problems, such as the more difficult preparation of nanogold, while solving the problems, such as that golden active surface area is small in Electrochemical Detection.
Description
Technical field
The present invention relates to biosensor preparation and electrochemical analysis techniques fields, and in particular to one kind can be used for DNA survey
The preparation and measuring method of fixed novel electrode.
Background technique
DNA (deoxyribonucleic acid) DNA is the important substance for regulating and controlling biological heredity.DNA piece
Specific nucleotide sequence in section determines the hereditary capacity of gene, and DNA variation may generate significant damage to organism.Cause
This, establish a kind of simple, quick, sensitive, high specificity DNA detection method be very it is necessary to.Currently, common DNA
Detection technique has the skills such as biochip technology, DNA sequencing technology, fluorescence spectrum, Surface enhanced Raman scattering, Electrochemical Detection
Art.Wherein, instrument complex and expensive needed for biochip technology, DNA sequencing technology, fluorescence spectrum, Surface enhanced Raman scattering is right
Personnel's operation is more demanding, and when check fee and instrument is larger is not easy to on-site test.The DNA detection electricity constructed in electrochemistry now
Pole is mostly hard material, and size shape is not easy to control, limits the extensive use of electrode.Nanometer material composition metal apply compared with
It is few, cause detection sensitivity not high.
Summary of the invention
It is flexible with flexible, higher sensitivity novel silver nanowires/nanogold that the purpose of the present invention is to provide a kind of
Combination electrode (AuNPs/AgNWs/PDMS electrode) and application the electrode preparation DNA biosensor and its be applied to measurement
The analysis method of DNA.
In order to solve the above technical problems, the present invention provides a kind of DNA biosensor.The DNA biosensor be by
Sulfydryl DNA is fixed on AuNPs/AgNWs/PDMS electrode.
The AuNPs/AgNWs/PDMS electrode the preparation method comprises the following steps:
Using PDMS as flexible substrates, with 2% polyvinyl alcohol (PVA) and 5% glycerine (Gly) mixed solution in its table
Hydrophilic surface layer is modified in face, is uniformly coated with one layer of silver nanowires conductive layer, on hydrophilic decorative layer with KAuCl4And H2SO4It mixes molten
Liquid is electrolyte, and Ag/AgCl electrode is reference electrode, and platinum filament is to electrode, and silver nanowires conductive layer is working electrode, using meter
When current method carried out on the conductive layer electrochemical deposition gold, prepare AuNPs/AgNWs/PDMS electrode.
Preferably, the KAuCl4And H2SO4In mixed electrolytic solution, KAuCl4Concentration is 0.01~0.05mol/L, H2SO4
Concentration is 0.1~0.5mol/L.
Preferably, electrochemical deposition gold current potential is -0.2V, and sedimentation time is 400~1600s.
The DNA biosensor the preparation method comprises the following steps:
(1) it is molten that the AuNPs/AgNWs/PDMS electrode is immersed to the PBS for containing sulfydryl single stranded DNA (SH-ssDNA) probe
It sufficiently reacts in liquid, then electrode is put into blank PBS, constant temperature stirring, to remove the DNA molecular of non-specific adsorption, so
It immerses in mercaptoethanol (MCH) afterwards, further eliminate non-specific adsorption DNA molecular and obtains relatively compact DNA unimolecule
Layer, after rinsed well with PBS solution and secondary distilled water, nitrogen is dry；
(2) the DNA probe electrode of mercaptoethanol (MCH) modified is immersed in the PBS solution of various concentration target DNA,
Water bath with thermostatic control is simultaneously stirred, and double-stranded DNA (dsDNA) modified electrode, secondary water and PBS solution repeated flushing is obtained, to remove surface
The non-hybridized target DNA of absorption, nitrogen is dry, spare.
Preferably, sulfydryl Single stranded DNA concentration is 10-7~10-8, Double stranded DNA concentration 10-7~10-9。
It is a further object to provide a kind of method using above-mentioned DNA biosensor measurement DNA, ssDNA
Or the measurement of the cyclic voltammetric signal of dsDNA modified electrode be in PBS (0.1M KCl) solution of 1mM methylene blue (MB) into
Capable.SsDNA or dsDNA modified electrode, which is placed in methylene blue (MB) solution of 1mM, impregnates 20min, it is ensured that methylene blue
(MB) and the abundant electrostatical binding of DNA chain.Then, with secondary distilled water, PBS (0.1M, pH 7.4) solution repeated flushing to remove
The molecule adsorbed on electrode surface.Then, the cyclic voltammetric signal being recorded in PBS (0.1M, pH 7.4) solution.Preferably,
Cyclic voltammetry experiment parameter is: potential range -0.6~0.6V；Sweep fast 100mV/s.
Compared with prior art, the invention has the following advantages:
The present invention provides it is a kind of conduct electricity very well, the flexible electrode that detection sensitivity is high.The technology for preparing electrode is simple,
Easily realize batch at production.Key point of the invention is that substrate PDMS is the flexible characteristic that flexible material determines electrode, Yin Na
Rice noodles have lower resistance, determine the excellent conductive performance of electrode.Nanogold particle close-packed arrays in silver nanowires are mercapto
Fixation of the base single stranded DNA on gold provides biggish surface area.Good electric conductivity and higher active surface area are to prepare
The guarantee of highly sensitive electrochemical sensor out.The present invention prepares equally distributed silver nanowires conduction using the method for drop coating
Then layer uses the method for electrochemical deposition to deposit nanogold particle in silver nanowires, maximum advantage is simply
It is easy, do not need synthesis nanogold, solve the problems, such as the more difficult preparation of nanogold.Solves gold activity in Electrochemical Detection simultaneously
The small problem of surface area.
Detailed description of the invention
Below in conjunction with attached drawing and specific embodiment, the present invention is further illustrated.
Fig. 1 is process flow chart of the invention；
Fig. 2 is flexible silver nanowires/nanogold combination electrode SEM image that the present invention prepares；
Fig. 3 is Electrochemical Characterization figure of the flexible silver nanowires/nanogold combination electrode in sulfuric acid；
Fig. 4 is silver nanowires electrode of the present invention, silver nanowires/nanogold combination electrode impedance contrast result；
Fig. 5 is silver nanowires electrode of the present invention, silver nanowires/nanogold combination electrode cyclic voltammetric comparing result.
Specific embodiment
Further description of the technical solution of the present invention in the following with reference to the drawings and specific embodiments, but the present invention not with
Any form constrained is in embodiment content.Experimental method described in embodiment is conventional method unless otherwise specified；Such as without spy
Different explanation, the experiment reagent and material, commercially obtain.
As shown in Figure 1, process flow chart of the invention.This method for flexible substrates, is used with dimethyl silicone polymer (PDMS)
Polyvinyl alcohol (PVA) and glycerine (Gly) mixed solution are in its surface modification hydrophilic layer, one layer of silver of even spread on hydrophilic layer
Nano wire conductive layer, and nanogold particle is deposited using the method for electrochemical deposition on the conductive layer.Novel silver is prepared to receive
DNA probe with sulfydryl is fixed in nanogold by rice noodles/nanogold electrode, is constructed DNA biosensor, is utilized methylene
Base indigo plant (MB) is that indicator quickly detects DNA concentration.
Embodiment 1
Using PVA (2%) and Gly (5%) mixed solution at PDMS surface modification 3 times, hydrophilic surface layer is obtained, in hydrophilic layer
Upper even spread silver nanowires conductive layer, uses KAuCl4(0.01mol/L) and H2SO4(0.5mol/L) mixed solution is electrolysis
Liquid, Ag/AgCl electrode are reference electrode, and platinum filament is to electrode, and silver nanowires conductive layer is working electrode.Using chrono-amperometric
Method, fixed deposition voltage are -0.2V, using the sedimentation time of 400s, carry out nanogold deposition.By electrochemical deposition, one layer
Golden flower-like nanometer gold will deposit on the electrically conductive, prepare silver nanowires/nanogold combination electrode.
Electrode immersion is contained 10-7In the PBS solution of sulfydryl single stranded DNA (SH-ssDNA) probe of mol/L, it is protected from light, room
Temperature is lower to react 12h.Then electrode is put into blank PBS, constant temperature stirs 1h at 37 DEG C, to remove the DNA of non-specific adsorption
Molecule.It is then immersed in 1h in 1mM MCH, further to eliminate non-specific adsorption DNA molecular and to obtain relatively compact DNA mono-
Molecular layer, after rinsed well with PBS solution and secondary distilled water, nitrogen drying for standby.
It is 10 that the DNA probe electrode of MCH modified, which is immersed in concentration,-7In the PBS solution of target DNA, 37 DEG C of waters bath with thermostatic control,
1.5h is stirred, double-stranded DNA (dsDNA) modified electrode is obtained.Secondary water and PBS solution repeated flushing, to remove adsorption
Non-hybridized target DNA, nitrogen is dry, spare.
(3) ssDNA or dsDNA modified electrode carries out cyclic voltammetric signal in PBS (0.1M KCl) solution of 1mM MB
Measurement.SsDNA or dsDNA modified electrode, which is placed in the methylene blue solution of 1mM, impregnates 20min, it is ensured that methylene blue and DNA
The abundant electrostatical binding of chain.Then, with secondary distilled water, PBS (0.1M, pH 7.4) solution repeated flushing to remove on electrode surface
The molecule of absorption, the cyclic voltammetric signal being recorded in PBS (0.1M, pH 7.4) solution except peroxide.CV experiment parameter is: electricity
- 0.6~0.6V of position；Sweep fast 100mV/s.
Embodiment 2
Using PVA (2%) and Gly (5%) mixed solution at PDMS surface modification 3 times, hydrophilic surface layer is obtained, in hydrophilic layer
Upper even spread silver nanowires conductive layer, uses KAuCl4(0.01mol/L) and H2SO4(0.5mol/L) mixed solution is electrolysis
Liquid, Ag/AgCl electrode are reference electrode, and platinum filament is to electrode, and silver nanowires conductive layer is working electrode.Using chrono-amperometric
Method, fixed deposition voltage are -0.2V, using the sedimentation time of 800s, carry out nanogold deposition.By electrochemical deposition, one layer
Golden flower-like nanometer gold will deposit on the electrically conductive, prepare silver nanowires/nanogold combination electrode.
Electrode immersion is contained 10-7In the PBS solution of sulfydryl single stranded DNA (SH-ssDNA) probe of mol/L, it is protected from light, room
Temperature is lower to react 12h.Then electrode is put into blank PBS, constant temperature stirs 1h at 37 DEG C, to remove the DNA of non-specific adsorption
Molecule.It is then immersed in 1h in 1mM MCH, further to eliminate non-specific adsorption DNA molecular and to obtain relatively compact DNA mono-
Molecular layer, after rinsed well with PBS solution and secondary distilled water, nitrogen drying for standby.
It is 10 that the DNA probe electrode of MCH modified, which is immersed in concentration,-8In the PBS solution of target DNA, 37 DEG C of waters bath with thermostatic control,
1.5h is stirred, double-stranded DNA (dsDNA) modified electrode is obtained.Secondary water and PBS solution repeated flushing, to remove adsorption
Non-hybridized target DNA, nitrogen is dry, spare.
(3) ssDNA or dsDNA modified electrode carries out cyclic voltammetric signal in PBS (0.1M KCl) solution of 1mM MB
Measurement.SsDNA or dsDNA modified electrode, which is placed in the methylene blue solution of 1mM, impregnates 20min, it is ensured that methylene blue and DNA
The abundant electrostatical binding of chain.Then, with secondary distilled water, PBS (0.1M, pH 7.4) solution repeated flushing to remove on electrode surface
The molecule of absorption, the cyclic voltammetric signal being recorded in PBS (0.1M, pH 7.4) solution of deoxygenation.CV experiment parameter is: electricity
- 0.6~0.6V of position；Sweep fast 100mV/s.
Embodiment 3
Using PVA (2%) and Gly (5%) mixed solution at PDMS surface modification 3 times, hydrophilic surface layer is obtained, in hydrophilic layer
Upper even spread silver nanowires conductive layer, uses KAuCl4(0.01mol/L) and H2SO4(0.5mol/L) mixed solution is electrolysis
Liquid, Ag/AgCl electrode are reference electrode, and platinum filament is to electrode, and silver nanowires conductive layer is working electrode.Using chrono-amperometric
Method, fixed deposition voltage are -0.2V, using the sedimentation time of 1600s, carry out nanogold deposition.By electrochemical deposition, one layer
Golden flower-like nanometer gold will deposit on the electrically conductive, prepare silver nanowires/nanogold combination electrode.
Electrode immersion is contained 10-8In the PBS solution of sulfydryl single stranded DNA (SH-ssDNA) probe of mol/L, it is protected from light, room
Temperature is lower to react 12h.Then electrode is put into blank PBS, constant temperature stirs 1h at 37 DEG C, to remove the DNA of non-specific adsorption
Molecule.It is then immersed in 1h in 1mM MCH, further to eliminate non-specific adsorption DNA molecular and to obtain relatively compact DNA mono-
Molecular layer, after rinsed well with PBS solution and secondary distilled water, nitrogen drying for standby.
It is 10 that the DNA probe electrode of MCH modified, which is immersed in concentration,-9In the PBS solution of target DNA, 37 DEG C of waters bath with thermostatic control,
1.5h is stirred, double-stranded DNA (dsDNA) modified electrode is obtained.Secondary water and PBS solution repeated flushing, to remove adsorption
Non-hybridized target DNA, nitrogen is dry, spare.
(3) ssDNA or dsDNA modified electrode carries out cyclic voltammetric signal in PBS (0.1M KCl) solution of 1mM MB
Measurement.SsDNA or dsDNA modified electrode, which is placed in the methylene blue solution of 1mM, impregnates 20min, it is ensured that methylene blue and DNA
The abundant electrostatical binding of chain.Then, with secondary distilled water, PBS (0.1M, pH 7.4) solution repeated flushing to remove on electrode surface
The molecule of absorption, the cyclic voltammetric signal being recorded in PBS (0.1M, pH 7.4) solution of deoxygenation.CV experiment parameter is: electricity
- 0.6~0.6V of position；Sweep fast 100mV/s.
Embodiment 4
Using PVA (2%) and Gly (5%) mixed solution at PDMS surface modification 3 times, hydrophilic surface layer is obtained, in hydrophilic layer
Upper even spread silver nanowires conductive layer, uses KAuCl4(0.01mol/L) and H2SO4(0.5mol/L) mixed solution is electrolysis
Liquid, Ag/AgCl electrode are reference electrode, and platinum filament is to electrode, and silver nanowires conductive layer is working electrode.Using chrono-amperometric
Method, fixed deposition voltage are -0.2V, using the sedimentation time of 800s, carry out nanogold deposition.By electrochemical deposition, one layer
Golden flower-like nanometer gold will deposit on the electrically conductive, prepare silver nanowires/nanogold combination electrode.
Electrode immersion is contained 10-7In the PBS solution of sulfydryl single stranded DNA (SH-ssDNA) probe of mol/L, it is protected from light, room
Temperature is lower to react 12h.Then electrode is put into blank PBS, constant temperature stirs 1h at 37 DEG C, to remove the DNA of non-specific adsorption
Molecule.It is then immersed in 1h in 1mM MCH, further to eliminate non-specific adsorption DNA molecular and to obtain relatively compact DNA mono-
Molecular layer, after rinsed well with PBS solution and secondary distilled water, nitrogen drying for standby.
It is 10 that the DNA probe electrode of MCH modified, which is immersed in concentration,-9In the PBS solution of target DNA, 37 DEG C of waters bath with thermostatic control,
1.5h is stirred, double-stranded DNA (dsDNA) modified electrode is obtained.Secondary water and PBS solution repeated flushing, to remove adsorption
Non-hybridized target DNA, nitrogen is dry, spare.
(3) ssDNA or dsDNA modified electrode carries out cyclic voltammetric signal in PBS (0.1M KCl) solution of 1mM MB
Measurement.SsDNA or dsDNA modified electrode, which is placed in the methylene blue solution of 1mM, impregnates 20min, it is ensured that methylene blue and DNA
The abundant electrostatical binding of chain.Then, with secondary distilled water, PBS (0.1M, pH 7.4) solution repeated flushing to remove on electrode surface
The molecule of absorption, the cyclic voltammetric signal being recorded in PBS (0.1M, pH 7.4) solution of deoxygenation.CV experiment parameter is: electricity
- 0.6~0.6V of position；Sweep fast 100mV/s.
As shown in Fig. 2, the SEM appearance structure image of electrode.Nanogold depositing homogeneous in silver nanowires, overlap joint are close.
As shown in figure 3, the electrochemical behavior of deposition gold (a) 800s, (b) 1200s, (c) 1600s combination electrode in sulfuric acid
As can be seen that the combination electrode active surface area of deposition gold 1600s is maximum.
Experiment is all made of deposition gold 1600s combination electrode below.
The combination electrode (b) of Fig. 4 silver nanowires/nanogold combination electrode (a), modified dsDNA.By impedance analysis,
After modified dsDNA, high frequency region small semicircle increases combination electrode, and low frequency range slope reduces.It is compound to know that existing DNA is fixed on
On electrode.
Fig. 5 silver nanowires/nanogold combination electrode (a), the combination electrode (b) of modified ssDNA, modified dsDNA
Combination electrode (c), the cyclic voltammogram after being soaked in 1mM MB PBS solution, in PBS (0.1M, pH 7.4) solution.By scheming
In it can be seen that (a) does not have peak in corresponding peak current (c) > (b) of -0.25V, illustrate that this electrode can carry out the detection of DNA.
Above is only a specific embodiment of the present invention, all equivalent replacements done according to scope of the present invention patent or
Change, is all covered by the protection scope of the present invention.
Claims (6)
1. a kind of DNA biosensor, which is characterized in that the DNA biosensor is that sulfydryl DNA is fixed on AuNPs/
On AgNWs/PDMS electrode；
The AuNPs/AgNWs/PDMS electrode the preparation method comprises the following steps:
Using PDMS as flexible substrates, with PVA and Gly mixed solution in its surface modification hydrophilic surface layer, on hydrophilic decorative layer
It is even to coat one layer of silver nanowires conductive layer, with KAuCl4And H2SO4Mixed solution is electrolyte, and Ag/AgCl electrode is reference electricity
Pole, platinum filament are to electrode, and silver nanowires conductive layer is working electrode, and electrochemistry is carried out on the conductive layer using chronoamperometry
Deposition gold, prepares AuNPs/AgNWs/PDMS electrode；
The DNA biosensor the preparation method comprises the following steps:
(1) the AuNPs/AgNWs/PDMS electrode is immersed in the PBS solution containing sulfydryl ssDNA probe and is sufficiently reacted,
Then electrode is put into blank PBS, constant temperature stirring to remove the DNA molecular of non-specific adsorption, is then immersed in mercaptoethanol
In, it further eliminates non-specific adsorption DNA molecular and obtains relatively compact DNA monolayer, it is rear with PBS solution and secondary
Distilled water flushing is clean, and nitrogen is dry；
(2) the DNA probe electrode of mercaptoethanol modified is immersed in the PBS solution of various concentration target DNA, water bath with thermostatic control is simultaneously
Stirring, obtains double-stranded DNA modified electrode, secondary water and PBS solution repeated flushing, to remove the non-hybridized target of adsorption
DNA, nitrogen is dry, spare.
2. a kind of method using DNA biosensor measurement DNA described in claim 1, which is characterized in that will be described single-stranded
DNA or double-stranded DNA modified electrode are placed in the methylene blue solution of 1mM and impregnate, with secondary distilled water, PBS solution repeated flushing
To remove the molecule adsorbed on electrode surface, then, the cyclic voltammetric signal being recorded in PBS solution.
3. a kind of DNA biosensor as described in claim 1, which is characterized in that in preparation AuNPs/AgNWs/PDMS electricity
In electrolyte needed for extremely, KAuCl4Concentration is 0.01~0.05mol/L, H2SO4Concentration is 0.1~0.5mol/L.
4. a kind of DNA biosensor as described in claim 1, which is characterized in that preparation AuNPs/AgNWs/PDMS electrode
When electrochemical deposition gold sedimentation potential be -0.2V, sedimentation time be 400~1600s.
5. a kind of DNA biosensor as described in claim 1, which is characterized in that sulfydryl Single stranded DNA concentration is 10-7~10-8, Double stranded DNA concentration 10-7~10-9。
6. a kind of method using DNA biosensor measurement DNA described in claim 1 as claimed in claim 2, special
Sign is, the cyclic voltammetry is swept and carried out under the conditions of fast 100mV/s in potential range -0.6~0.6V.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811239868.1A CN109355356B (en) | 2018-10-23 | 2018-10-23 | DNA biosensor and method for applying same to DNA determination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811239868.1A CN109355356B (en) | 2018-10-23 | 2018-10-23 | DNA biosensor and method for applying same to DNA determination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109355356A true CN109355356A (en) | 2019-02-19 |
| CN109355356B CN109355356B (en) | 2022-05-27 |
Family
ID=65346396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811239868.1A Active CN109355356B (en) | 2018-10-23 | 2018-10-23 | DNA biosensor and method for applying same to DNA determination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109355356B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151584A (en) * | 2021-01-11 | 2021-07-23 | 南通大学 | SARS-CoV-2 detection kit and detection method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102072931A (en) * | 2010-12-09 | 2011-05-25 | 华东师范大学 | Method for preparing biosensor based on silicon nanowires and application of biosensor to detecting DNA |
| CN104630869A (en) * | 2015-01-22 | 2015-05-20 | 江南大学 | DNA sensor for detecting staphylococcus aureus as well as preparation method and application of DNA sensor |
-
2018
- 2018-10-23 CN CN201811239868.1A patent/CN109355356B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102072931A (en) * | 2010-12-09 | 2011-05-25 | 华东师范大学 | Method for preparing biosensor based on silicon nanowires and application of biosensor to detecting DNA |
| CN104630869A (en) * | 2015-01-22 | 2015-05-20 | 江南大学 | DNA sensor for detecting staphylococcus aureus as well as preparation method and application of DNA sensor |
Non-Patent Citations (2)
| Title |
|---|
| CHEN HAO等: "Gold nanoparticles decorated silver-nanowire films for transparent electrode with excellent thermal stability", 《MATERIALS LETTERS》 * |
| 杨海波等: "银纳米线柔性电极的制备及电化学方法测定水中微量铜离子", 《分析化学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151584A (en) * | 2021-01-11 | 2021-07-23 | 南通大学 | SARS-CoV-2 detection kit and detection method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109355356B (en) | 2022-05-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Amemiya et al. | Scanning electrochemical microscopy | |
| CN108802141B (en) | Electrochemical glucose sensor and its detection method without enzyme | |
| Zuaznabar-Gardona et al. | A wide-range solid state potentiometric pH sensor based on poly-dopamine coated carbon nano-onion electrodes | |
| CN105842312B (en) | Nanometer flower-shaped ultramicro gold electrode and preparation and application thereof | |
| CN104760922B (en) | A kind of ultra micro planar electrode array sensor and preparation method thereof | |
| Quinton et al. | On-chip multi-electrochemical sensor array platform for simultaneous screening of nitric oxide and peroxynitrite | |
| Shen et al. | Electrochemical nanoprobes for the chemical detection of neurotransmitters | |
| Seo et al. | Microfluidic neurotransmitters sensor in blood plasma with mediator-immobilized conducting polymer/N, S-doped porous carbon composite | |
| CN105866205A (en) | Establishment and application of electrochemical DNA biosensor based on gold nanoparticle-sulfhydryl graphene modified electrode | |
| Ghoreishi et al. | Fabrication of a graphene oxide nano-sheet modified electrode for determination of dopamine in the presence of tyrosine: A multivariate optimization strategy | |
| Mariani et al. | Micro-and nano-devices for electrochemical sensing | |
| Amatore et al. | Vesicular exocytosis and microdevices–microelectrode arrays | |
| Lamaka et al. | Novel Solid‐Contact Ion‐Selective Microelectrodes for Localized Potentiometric Measurements | |
| CN109211989A (en) | A kind of electrochemical aptamer sensor and its preparation and detection method for detecting Atrazine | |
| Sen | Using electropolymerization-based doping for the electro-addressable functionalization of a multi-electrode array probe for nucleic acid detection | |
| Shamsipur et al. | Impedimetric sensing of cesium ion based on a thiacalix [4] arene self-assembled gold electrode | |
| Iwama et al. | Fabrication of High-Density Vertical Closed Bipolar Electrode Arrays by Carbon Paste Filling Method for Two-Dimensional Chemical Imaging | |
| CN103743801A (en) | Droplet-microfluidic-based preparation method of platinum black-modified electrode biosensor and application thereof | |
| Hu et al. | Simultaneous determination of dopamine and ascorbic acid using the nano‐gold self‐assembled glassy carbon electrode | |
| CN109355356A (en) | A kind of DNA biosensor and its method applied to measurement DNA | |
| De Vitre et al. | Preparation and properties of a mercury-plated iridium-based microelectrode | |
| Liu et al. | Synthesis and characterization of a highly stable poly (3, 4-ethylenedioxythiophene)-gold nanoparticles composite film and its application to electrochemical dopamine sensors | |
| Ferrario et al. | Development of a disposable gold electrodes-based sensor for electrochemical measurements of cDNA hybridization | |
| CN110261450A (en) | It is a kind of to detect dopamine and adrenaline modified glassy carbon electrode, preparation method and application simultaneously | |
| JP5230741B2 (en) | Two-dimensional control of electrochemical surface potential |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |