CN109355218A - It is rapidly heated the Multifunctional fermentation composite bacteria agent and the preparation method and application thereof of taste removal for livestock excrement composting - Google Patents
It is rapidly heated the Multifunctional fermentation composite bacteria agent and the preparation method and application thereof of taste removal for livestock excrement composting Download PDFInfo
- Publication number
- CN109355218A CN109355218A CN201811214539.1A CN201811214539A CN109355218A CN 109355218 A CN109355218 A CN 109355218A CN 201811214539 A CN201811214539 A CN 201811214539A CN 109355218 A CN109355218 A CN 109355218A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- fermentation
- bacteria agent
- composite bacteria
- nitrobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
Abstract
Be rapidly heated the Multifunctional fermentation composite bacteria agent and the preparation method and application thereof of taste removal the invention discloses a kind of for livestock excrement composting, the composite bacteria agent include bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and Nitrobacillus harbinensis BM62 solid fungicide.Composite bacteria agent of the invention is the multiple-microorganism composite microbial system having complementary functions, have both Rapid Fermentation heat production, biological deodorizing protects nitrogen, the multiple efficacies such as the decomposed degradation of compost material, livestock excrement composting can be made to be rapidly heated, it is substantially reduced unhappy smell, reduce nitrogen loss in compost, the organic substances such as fast and effective decomposition of cellulose, to accelerate the decomposed speed of compost material, promote quality of compost products, it can be used for producing biological organic fertilizer, reduce agriculture production cost, environmental pollution caused by feces of livestock and poultry is solved simultaneously, with good application value and social effect.
Description
Technical field
The invention belongs to environmental applications microorganism fields, and in particular to one kind is rapidly heated taste removal for livestock excrement composting
Multifunctional fermentation composite bacteria agent and the preparation method and application thereof.
Background technique
China's animal husbandry development is rapid in recent years, and a large amount of feces of livestock and poultry generated therewith are not utilized effectively not only,
And it causes serious environmental pollution.Although using livestock excrement composting being the ideal way of resource utilization organic waste
Diameter, but there are still many problems to cause air pollution such as the Ammonia valatilization in composting process while reduce fertilizer efficiency；The heating of heap body
Slowly, lead to that the degradation of the organic macromolecules such as cellulose is insufficient and extends the compost maturity time etc..For these problems, usually adopt
Ammonia volatilization is controlled with the method for microorganisms with specific functions is added into compost to improve compost nitrogen content, heap body is promoted quickly to rise
Temperature accelerates organic matter degradation, to accelerate compost maturity process, improve quality of compost products, and effectively reduces environmental pollution.
Common problem is to lack basic theoretical direction during complex microorganism screening and culturing, lead at present
Cause the bacterial strain functionality filtered out not strong；Moreover, the interaction between fermentation process and its each bacterial strain to compound criteria
Solution is insufficient, causes product total viable count not high, and compost is made not reach the ideal effect for promoting maturity using composite bacteria agent.
Summary of the invention
In response to this problem, the purpose of the present invention is to provide one kind have so that livestock excrement composting is rapidly heated, taste removal, subtract
Few nitrogen loss improves being rapidly heated the multi-functional hair of taste removal for livestock excrement composting for composting efficiency and quality of compost products
Ferment composite bacteria agent and the preparation method and application thereof.In order to achieve the object of the present invention, it is proposed to adopt the following technical solutions:
Firstly, the present invention provide it is a kind of be rapidly heated the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting,
Including bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and Nitrobacillus
Harbinensis BM62, the composite bacteria agent are solid fungicide.
Secondly, the present invention is provided and above-mentioned is rapidly heated the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting
Preparation method includes the following steps:
(1) inclined-plane culture activates: by bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like gemma bar
The strain of bacterium and Nitrobacillus harbinensis BM62 are inoculated in respectively on corresponding slant medium, activation training
It supports, makes actication of culture；
(2) first order seed culture: the strain that step (1) activates is inoculated into fluid nutrient medium shake culture respectively, is obtained
Primary seed solution；
(3) secondary seed culture: the primary seed solution that step (2) obtains is inoculated into fermentation medium respectively, fermentation
It ferments in tank, obtains secondary seed solution；
(4) strain solid fermentation culture: the secondary seed solution that step (3) obtains being inoculated into wheat bran, is dried after fermentation,
Obtain solid fungicide；
(5) prepared by composite bacteria agent: by bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like gemma bar
The mixing of the solid fungicide of bacterium and Nitrobacillus harbinensis BM62, is made and is rapidly heated for livestock excrement composting
The Multifunctional fermentation composite bacteria agent of taste removal.
Activation culture described in step (1), bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like bud
Spore bacillus is inoculated in beef extract-peptone slant medium, and 37 DEG C are cultivated 2 days；Nitrobacillus harbinensis BM62
It is inoculated in ammonia oxidation bacteria slant medium, 50 DEG C are cultivated 3 days.
Step (2) described fluid nutrient medium, bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like bud
The fluid nutrient medium of spore bacillus is beef extract-peptone fluid nutrient medium；The liquid of Nitrobacillus harbinensis BM62
Body culture medium is ammonia oxidation bacteria fluid nutrient medium.
Step (2) described shake culture, bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like gemma
Bacillus cultivates 24-48h in 37 DEG C, 150r/min；Nitrobacillus harbinensis BM62 is trained in 50 DEG C, 120r/min
Support 72-96h.
Step (3) described fermentation medium, bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like bud
The fermentation medium of spore bacillus is beef extract-peptone fluid nutrient medium；The hair of Nitrobacillus harbinensis BM62
Ferment culture medium is ammonia oxidation bacteria fluid nutrient medium.
Step (3) described inoculation, the primary seed solution of inoculation account for the 10-15% of fermentation medium volume；Step (3) is described
Fermentation, bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus are in 30-37 DEG C, mixing speed
For 150-200r/min, ventilatory capacity 1:(1-1.5) (V/Vmin), cultivate 2-3 days；Nitrobacillus
For harbinensis BM62 in 50 DEG C, mixing speed 120-150r/min, ventilatory capacity is (0.5-1): 1 (V/Vmin), training
It supports 4-5 days.
The volume/mass ratio of step (4) described inoculation, secondary seed solution and wheat bran is 1: 3 (mL/g)；The wheat bran is to go out
Bacterium wheat bran；Fermentation condition is room temperature fermentation, fermentation time 24-48h.
Step (5) bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and
The mass ratio of the solid fungicide of Nitrobacillus harbinensis BM62 is (1-2): (1-2): (1-2): (1-2): (1-
2)。
Third is rapidly heated the Multifunctional fermentation composite bacteria agent of taste removal the present invention also provides above-mentioned for livestock excrement composting
Application, the composite bacteria agent be used for feces of livestock and poultry and agricultural crop straw compost, include the following steps:
1) rice straw is cut up with a hay cutter into section, is uniformly mixed with freshly-slaughtered poultry excrement, adjust moisture content；
2) inoculation is rapidly heated the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting, after mixing compost
Fermentation.
The application specifically:
1) composting material be fresh chicken manure and rice straw, by rice straw hand hay cutter at 3-5cm segment, with freshly-slaughtered poultry excrement according to
Mass ratio 1:(3-4) is uniformly mixed, and adjusts moisture content to 60~65% (wt)；
2) microbial inoculum is added in Jian Dui, the amount of the microbial inoculum is (8-9) × 109CFU·kg-1Composting material, after inoculation
Even mixing, compost fermentation；The turning when temperature rises to 45-65 DEG C, every 3d turning 1 time, temperature are every after dropping to 35 DEG C or less
5d is turned over 1 time, until heap temperature drops to environment temperature and terminates to ferment.
Beneficial effect
Composite bacteria agent of the invention is the multiple-microorganism composite microbial system having complementary functions, and has both Rapid Fermentation heat production, biology
The multiple efficacies such as deodorizing and nitrogen protecting, fast and effective decomposition of cellulose macromolecular, can make livestock excrement composting be rapidly heated, be substantially reduced
Unhappy smell reduces nitrogen loss in compost, reduces hardly degraded organic substance content, to accelerate the decomposed speed of compost material
Degree promotes quality of compost products.
Detailed description of the invention
Fig. 1: the variation of temperature in 1 Composting Process of embodiment；
Fig. 2: the variation of temperature in 2 Composting Process of embodiment；
Fig. 3: the variation of temperature in 3 Composting Process of embodiment.
Specific embodiment
For material and technique involved in the technical solution that the present invention is further explained, following embodiment is given.This
A little embodiments do not limit the scope of the invention in any way.
In following implementation, bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus,
The source of Nitrobacillus harbinensis BM62 is respectively as follows:
The bacillus subtilis purchase of in September, (1.15792) 2017 is in China General Microbiological culture presevation administrative center
(CGMCC)；
The purchase of in September, (1.3656) 2017 of Mo Hawei bacillus is in China General Microbiological culture presevation administrative center
(CGMCC)；
Skin spy acinetobacter calcoaceticus in June, (10526) 2017 is bought in Chinese industrial Microbiological Culture Collection administrative center
(CICC)；
The Bacillus cercus purchase of in September, (1.10559) 2017 is in China General Microbiological culture presevation administrative center
(CGMCC)；
Nitrobacillus harbinensis BM62 is a kind of heat-resistant bacillus, classification naming
Nitrobacillus harbinensis, laboratory number BM62, is deposited in China General Microbiological culture presevation administrative center
(CGMCC), deposit number CGMCC No.16237, preservation date on August 13rd, 2018, preservation address was Chaoyang District, Beijing City north
The institute 3 of occasion West Road 1.
Culture medium prescription involved in following implementation are as follows:
Beef extract-peptone slant medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000mL；
Ammonia oxidation bacteria slant medium: (NH4)2SO45g, KCl 4g, MgSO42.5g, CaCl21.85g, NaCl 3g,
NaHCO342g, peptone 60g, beef extract 40g, agar 20g, distilled water 1000mL；
Ammonia oxidation bacteria fluid nutrient medium: (NH4)2SO45g, KCl 4g, MgSO42.5g, CaCl21.85g, NaCl 3g,
NaHCO342g, peptone 60g, beef extract 40g, distilled water 1000mL；
Beef extract-peptone fluid nutrient medium: beef extract 3g, peptone 10g, NaCl 5g, water 1000mL.
Embodiment 1: it is rapidly heated the preparation and application of the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting
It is rapidly heated the preparation of the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting:
(1) inclined-plane culture activates: by bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like gemma bar
The strain of bacterium and Nitrobacillus harbinensis BM62 are inoculated in respectively on corresponding slant medium, activation training
It supports, makes actication of culture；
(2) first order seed culture: the strain that step (1) activates is inoculated into fluid nutrient medium shake culture respectively, is obtained
Primary seed solution；
(3) secondary seed culture: the primary seed solution that step (2) obtains is inoculated into fermentation medium respectively, fermentation
It ferments in tank, obtains secondary seed solution；
(4) strain solid fermentation culture: the secondary seed solution that step (3) obtains being inoculated into wheat bran, is dried after fermentation,
Obtain solid fungicide；
(5) prepared by composite bacteria agent: by bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like gemma bar
The mixing of the solid fungicide of bacterium and Nitrobacillus harbinensis BM62, is made and is rapidly heated for livestock excrement composting
The Multifunctional fermentation composite bacteria agent of taste removal.
Activation culture described in step (1), bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like bud
Spore bacillus is inoculated in beef extract-peptone slant medium, and 37 DEG C are cultivated 2 days；Nitrobacillus harbinensis BM62
It is inoculated in ammonia oxidation bacteria slant medium, 50 DEG C are cultivated 3 days；
Step (2) described fluid nutrient medium, bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like bud
The fluid nutrient medium of spore bacillus is beef extract-peptone fluid nutrient medium；The liquid of Nitrobacillus harbinensis BM62
Body culture medium is ammonia oxidation bacteria fluid nutrient medium；Step (2) described shake culture, bacillus subtilis, Mo Hawei bacillus,
Skin spy acinetobacter calcoaceticus, Bacillus cercus cultivate 36h in 37 DEG C, 150r/min；Nitrobacillus harbinensis
BM62 cultivates 80h in 50 DEG C, 120r/min.
Step (3) described fermentation medium, bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, wax-like bud
The fermentation medium of spore bacillus is beef extract-peptone fluid nutrient medium；The hair of Nitrobacillus harbinensis BM62
Ferment culture medium is ammonia oxidation bacteria fluid nutrient medium；Step (3) described inoculation, the primary seed solution of inoculation account for fermentation medium volume
12%；Step (3) described fermentation, bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus
In 34 DEG C, mixing speed 175r/min, ventilatory capacity is 1:1 (V/Vmin), is cultivated 2 days；Nitrobacillus
For harbinensis BM62 in 50 DEG C, mixing speed 130r/min, ventilatory capacity is 1:1 (V/Vmin), is cultivated 4 days.
The volume/mass ratio of step (4) described inoculation, secondary seed solution and wheat bran is 1: 3 (mL/g)；The wheat bran is to go out
Bacterium wheat bran；Fermentation condition is room temperature fermentation, and fermentation time is for 24 hours.
Step (5) bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and
The mass ratio of the solid fungicide of Nitrobacillus harbinensis BM62 is 1:1:1:1:1:1.
It is rapidly heated the application of the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting:
Composting material is fresh chicken manure and rice straw (conditioner).It is and fresh by rice straw hand hay cutter at the segment of 4cm or so
Chicken manure is uniformly mixed according to the mass ratio of 1:3-4, adjusts moisture content to 65% (wt).The windrow content of organic matter is about
59.5% (wt), C/N (mass ratio) is about 30:1.
Compost and the method for sampling:
Windrow divides A, B group, and A group is control sample, carries out Natural compost；B group adds the composite bacteria agent of this embodiment preparation.It adopts
With solid-state inoculum, concentration is 8-9 × 109CFU·kg-1, inoculum concentration 20gkg-1, uniform hybrid reactor body material after inoculation,
It is placed in 30cm × 40cm × 50cm round.When temperature rises to 45 DEG C of beginning turnings, every 3d turning 1 time, at a temperature of
Every 5d is turned over 1 time after dropping to 35 DEG C, until heap temperature drops to environment temperature and terminates to ferment, to ensure that windrow obtains sufficient oxygen
Gas.At interval of 24 hours measurements content of cellulose, NH3Volatile quantity, total N andContent, all analytical samplings acquire 3 and put down
Row sample.
Experimental result
The variation of temperature in composting process
Inoculation microbial inoculum enters pyrolytic stage (> 45 DEG C) (see Fig. 1) in compost the 2nd day after composting, and continues to
12nd day, temperature was gradually reduced later, and 20 days whens are down to 35 DEG C or less.In entire composting process, the megathermal period (2~12d) is maintained
11 days；Although the heap temperature of Natural compost is higher than room temperature, the megathermal period not can enter within entire experimental period.It can be seen that connecing
Kind of microbial inoculum can significantly improve in Composting Process, especially the microbial metabolic activity of compost early period, so that heap temperature is improved,
Temperature guarantee is provided for the degradation of organic matter macromolecular, accelerates compost maturity process.
Cellulose degradation rate in composting process, total N,Content and NH3Volatile quantity variation
As shown in Table 1, it at the end of compost, compared with compareing compost, adds cellulose degradation rate in the compost of microbial inoculum and improves
19.83%, total nitrogen loss rate reduces by 10%,Content increases by 46.6%, NH3Volatility reduces by 35.5%, shows that this is compound
Function microbial inoculum can effectively facilitate cellulose substances in compost and degrade, and reduce NH3Volatilization increasesContent, to reduce
Bad smell is effectively reduced in nitrogen loss.
Cellulose degradation rate in 1 composting process of table, total N,NH3Volatile quantity variation
Embodiment 2: it is rapidly heated the preparation and application of the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting
Difference from Example 1 is mixed proportion when composite bacteria agent preparation are as follows: bacillus subtilis, Mo Hawei bud
Spore bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and Nitrobacillus harbinensis BM62 solid fungicide according to
The mass ratio of 2:2:1:1:1 mixes.
Composting material and method, sampling and measuring method are same as Example 1.
Experimental result
The variation of temperature in composting process
The compost of inoculation microbial inoculum also enters pyrolytic stage (> 45 DEG C) (see Fig. 2) in the 2nd day after composting,
Megathermal period continue for 14 days, extend 3 days than embodiment 1.
Cellulose degradation rate in composting process, total N,Content and NH3Volatile quantity variation
As shown in Table 2, it at the end of compost, compared with compareing compost, adds cellulose degradation rate in the compost of microbial inoculum and improves
21.73%, total nitrogen loss rate reduces 8.1%,Content increases by 39.7%, NH3Volatilization reduces 23.1%.
Cellulose degradation rate in 2 composting process of table, total N,NH3Volatile quantity variation
Embodiment 3: it is rapidly heated the preparation and application of the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting
Difference from Example 1 is mixed proportion when composite bacteria agent preparation are as follows: bacillus subtilis, Mo Hawei bud
Spore bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and Nitrobacillus harbinensis BM62 solid fungicide according to
The mass ratio of 1:2:1:2:1 mixes.
Composting material and method, sampling and measuring method are same as Example 1.
Experimental result
The variation of temperature in composting process
The compost of inoculation microbial inoculum also enters pyrolytic stage (> 45 DEG C) (see Fig. 3) in the 2nd day after composting,
Megathermal period continue for 14 days, extend 3 days than embodiment 1.
Cellulose degradation rate in composting process, total N,Content and NH3Volatile quantity variation
Cellulose degradation rate in 3 composting process of table, total N,NH3Volatile quantity variation
As shown in Table 3, it at the end of compost, compared with compareing compost, adds cellulose degradation rate in the compost of microbial inoculum and improves
18.86%, total nitrogen loss rate reduces 11.2%,Content increases 49.1%, NH3Volatilization reduces 42.6%.
Claims (10)
1. a kind of be rapidly heated the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting, it is characterised in that: including withered
Careless bacillus, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and Nitrobacillus
Harbinensis BM62, the composite bacteria agent are solid fungicide.
2. it is a kind of it is described in claim 1 for livestock excrement composting be rapidly heated taste removal Multifunctional fermentation composite bacteria agent system
Preparation Method, characterized by the following steps:
(1) inclined-plane culture activate: by bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and
The strain of Nitrobacillus harbinensis BM62 is inoculated in respectively on corresponding slant medium, and activation culture makes
Actication of culture；
(2) first order seed culture: the strain that step (1) activates is inoculated into fluid nutrient medium shake culture respectively, obtains level-one
Seed liquor；
(3) secondary seed culture: the primary seed solution that step (2) obtains is inoculated into fermentation medium respectively, in fermentor
Fermentation, obtains secondary seed solution；
(4) strain solid fermentation culture: the secondary seed solution that step (3) obtains is inoculated into wheat bran, dries, obtains after fermentation
Solid fungicide；
(5) composite bacteria agent prepare: by bacillus subtilis, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and
The solid fungicide of Nitrobacillus harbinensis BM62 mixes, and is made and is rapidly heated taste removal for livestock excrement composting
Multifunctional fermentation composite bacteria agent.
3. preparation method according to claim 2, it is characterised in that: activation culture described in step (1), bacillus subtilis
Bacterium, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus are inoculated in beef extract-peptone slant medium, and 37 DEG C
Culture 2 days；Nitrobacillus harbinensis BM62 is inoculated in ammonia oxidation bacteria slant medium, and 50 DEG C are cultivated 3 days.
4. preparation method according to claim 2, it is characterised in that: step (2) described fluid nutrient medium, bacillus subtilis
Bacterium, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus fluid nutrient medium be beef extract-peptone Liquid Culture
Base；The fluid nutrient medium of Nitrobacillus harbinensis BM62 is ammonia oxidation bacteria fluid nutrient medium.
5. preparation method according to claim 2, it is characterised in that: step (2) described shake culture, bacillus subtilis
Bacterium, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus cultivate 24-48h in 37 DEG C, 150r/min；
Nitrobacillus harbinensis BM62 cultivates 72-96h in 50 DEG C, 120r/min.
6. preparation method according to claim 2, it is characterised in that: step (3) described fermentation medium, bacillus subtilis
Bacterium, Mo Hawei bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus fermentation medium be beef extract-peptone Liquid Culture
Base；The fermentation medium of Nitrobacillus harbinensis BM62 is ammonia oxidation bacteria fluid nutrient medium.
7. preparation method according to claim 2, it is characterised in that: step (3) described inoculation, the primary seed solution of inoculation
Account for the 10-15% of fermentation medium volume；Step (3) described fermentation, bacillus subtilis, Mo Hawei bacillus, Pi Te are not
Lever bacterium, Bacillus cercus are in 30-37 DEG C, mixing speed 150-200r/min, ventilatory capacity 1:(1-1.5) (V/V
Min), cultivate 2-3 days；Nitrobacillus harbinensis BM62 leads in 50 DEG C, mixing speed 120-150r/min
Tolerance is (0.5-1): 1 (V/Vmin), is cultivated 4-5 days.
8. preparation method according to claim 2, it is characterised in that: step (4) described inoculation, secondary seed solution and wheat bran
Volume/mass ratio be 1: 3 (mL/g)；The wheat bran is sterilizing wheat bran；Fermentation condition is room temperature fermentation, fermentation time 24-
48h。
9. preparation method according to claim 2, it is characterised in that: step (5) bacillus subtilis, Mo Hawei bud
Spore bacillus, skin spy acinetobacter calcoaceticus, Bacillus cercus and Nitrobacillus harbinensis BM62 solid fungicide
Mass ratio is (1-2): (1-2): (1-2): (1-2): (1-2).
It described in claim 1 is rapidly heated the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting 10. a kind of
Using, it is characterised in that: the composite bacteria agent is used for feces of livestock and poultry and agricultural crop straw compost, includes the following steps:
1) rice straw is cut up with a hay cutter into section, is uniformly mixed with freshly-slaughtered poultry excrement, adjust moisture content；
2) inoculation is rapidly heated the Multifunctional fermentation composite bacteria agent of taste removal for livestock excrement composting, and compost is sent out after mixing
Ferment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811214539.1A CN109355218A (en) | 2018-10-18 | 2018-10-18 | It is rapidly heated the Multifunctional fermentation composite bacteria agent and the preparation method and application thereof of taste removal for livestock excrement composting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811214539.1A CN109355218A (en) | 2018-10-18 | 2018-10-18 | It is rapidly heated the Multifunctional fermentation composite bacteria agent and the preparation method and application thereof of taste removal for livestock excrement composting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109355218A true CN109355218A (en) | 2019-02-19 |
Family
ID=65345736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811214539.1A Withdrawn CN109355218A (en) | 2018-10-18 | 2018-10-18 | It is rapidly heated the Multifunctional fermentation composite bacteria agent and the preparation method and application thereof of taste removal for livestock excrement composting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109355218A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110040927A (en) * | 2019-04-28 | 2019-07-23 | 青岛兴开环境科技有限公司 | A method of livestock and poultry feces are handled using composite flora |
| CN110791446A (en) * | 2019-11-19 | 2020-02-14 | 森诺技术有限公司 | Ecological toilet treatment strain and application thereof |
| CN111748495A (en) * | 2020-07-02 | 2020-10-09 | 农业农村部规划设计研究院 | Initiating agent for cow dung aerobic fermentation and preparation method thereof |
| CN112481176A (en) * | 2021-01-11 | 2021-03-12 | 山西省农业科学院农业环境与资源研究所 | Decomposing inoculant for branch composting and preparation method thereof |
| CN112725214A (en) * | 2020-10-23 | 2021-04-30 | 江西农业大学 | Biogas residue aerobic compost composite microbial agent and application thereof |
| CN112725239A (en) * | 2021-02-05 | 2021-04-30 | 宁夏大学 | Decomposing inoculant with cellulose degradation and deodorization functions as well as preparation method and application thereof |
| CN113122483A (en) * | 2021-05-31 | 2021-07-16 | 哈尔滨工业大学 | Rapid heat-producing microbial inoculum for aerobic composting of corn straws and application of rapid heat-producing microbial inoculum |
| CN113174386A (en) * | 2021-04-27 | 2021-07-27 | 新至农业生态科技有限公司 | Composite fermentation inoculant for manure composting |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103228264A (en) * | 2010-06-24 | 2013-07-31 | 诺维信生物股份有限公司 | Methods and compositions for reducing body odor |
| CN103421824A (en) * | 2013-08-28 | 2013-12-04 | 华中农业大学 | Resistance gene capable of degrading herbicide glyphosate and encoded protein of resistance gene |
| CN103497915A (en) * | 2013-09-22 | 2014-01-08 | 东北农业大学 | Complex microbial agent for composting fermentation and application thereof |
| CN103960461A (en) * | 2014-04-24 | 2014-08-06 | 武汉虹睿生物科技开发有限公司 | Application of adopting Mojave Bacillus KJS-3 as feed additive |
| CN104101840A (en) * | 2013-04-03 | 2014-10-15 | 株式会社杰士汤浅国际 | State estimation device and state estimation method |
| WO2017087234A1 (en) * | 2015-11-18 | 2017-05-26 | Empire Technology Development Llc | Methods and compositions for treating and preventing dag formation |
| CN107384842A (en) * | 2017-09-22 | 2017-11-24 | 广东工业大学 | A kind of composite bacteria and its application, composite bacteria agent |
| CN108148790A (en) * | 2018-03-07 | 2018-06-12 | 苏州顶裕节能设备有限公司 | A kind of mixed bacteria, carrier and the method for livestock and poultry biology dejection degradation |
-
2018
- 2018-10-18 CN CN201811214539.1A patent/CN109355218A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103228264A (en) * | 2010-06-24 | 2013-07-31 | 诺维信生物股份有限公司 | Methods and compositions for reducing body odor |
| CN104101840A (en) * | 2013-04-03 | 2014-10-15 | 株式会社杰士汤浅国际 | State estimation device and state estimation method |
| CN103421824A (en) * | 2013-08-28 | 2013-12-04 | 华中农业大学 | Resistance gene capable of degrading herbicide glyphosate and encoded protein of resistance gene |
| CN103497915A (en) * | 2013-09-22 | 2014-01-08 | 东北农业大学 | Complex microbial agent for composting fermentation and application thereof |
| CN103960461A (en) * | 2014-04-24 | 2014-08-06 | 武汉虹睿生物科技开发有限公司 | Application of adopting Mojave Bacillus KJS-3 as feed additive |
| WO2017087234A1 (en) * | 2015-11-18 | 2017-05-26 | Empire Technology Development Llc | Methods and compositions for treating and preventing dag formation |
| CN107384842A (en) * | 2017-09-22 | 2017-11-24 | 广东工业大学 | A kind of composite bacteria and its application, composite bacteria agent |
| CN108148790A (en) * | 2018-03-07 | 2018-06-12 | 苏州顶裕节能设备有限公司 | A kind of mixed bacteria, carrier and the method for livestock and poultry biology dejection degradation |
Non-Patent Citations (4)
| Title |
|---|
| JIE XU等: "Evaluation of a novel thermophilic nitrifying Bacillaceae species Aliibacillus thermotolerans BM62T promoting nitrogen retention in livestock manure compost", 《ENVIRONMENTAL PROGRESS AICHE & SUSTAINABLE ENERGY》 * |
| KONDAKINDI VENKATESWAR REDDY等: "Characterization of Some Efficient Cellulase Producing Bacteria Isolated from Pulp and Paper Mill Effluent Contaminated Soil", 《BIOLOGICAL AND APPLIED SCIENCES》 * |
| 徐杰等: "畜禽粪便堆肥快速升温除臭多功能复合菌剂及其应用", 《中国土壤与肥料》 * |
| 时涛等: "橡胶树内生细菌多样性初探及拮抗菌株的筛选", 《中国森林病虫》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110040927A (en) * | 2019-04-28 | 2019-07-23 | 青岛兴开环境科技有限公司 | A method of livestock and poultry feces are handled using composite flora |
| CN110791446A (en) * | 2019-11-19 | 2020-02-14 | 森诺技术有限公司 | Ecological toilet treatment strain and application thereof |
| CN110791446B (en) * | 2019-11-19 | 2021-07-27 | 森诺技术有限公司 | Ecological toilet treatment strain and application thereof |
| CN111748495A (en) * | 2020-07-02 | 2020-10-09 | 农业农村部规划设计研究院 | Initiating agent for cow dung aerobic fermentation and preparation method thereof |
| CN111748495B (en) * | 2020-07-02 | 2022-04-26 | 农业农村部规划设计研究院 | Initiating agent for cow dung aerobic fermentation and preparation method thereof |
| CN112725214A (en) * | 2020-10-23 | 2021-04-30 | 江西农业大学 | Biogas residue aerobic compost composite microbial agent and application thereof |
| CN112725214B (en) * | 2020-10-23 | 2022-09-23 | 江西农业大学 | Biogas residue aerobic compost composite microbial agent and application thereof |
| CN112481176A (en) * | 2021-01-11 | 2021-03-12 | 山西省农业科学院农业环境与资源研究所 | Decomposing inoculant for branch composting and preparation method thereof |
| CN112725239A (en) * | 2021-02-05 | 2021-04-30 | 宁夏大学 | Decomposing inoculant with cellulose degradation and deodorization functions as well as preparation method and application thereof |
| CN113174386A (en) * | 2021-04-27 | 2021-07-27 | 新至农业生态科技有限公司 | Composite fermentation inoculant for manure composting |
| CN113174386B (en) * | 2021-04-27 | 2022-06-24 | 新至农业生态科技有限公司 | Composite fermentation microbial inoculum for manure compost |
| CN113122483A (en) * | 2021-05-31 | 2021-07-16 | 哈尔滨工业大学 | Rapid heat-producing microbial inoculum for aerobic composting of corn straws and application of rapid heat-producing microbial inoculum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109355218A (en) | It is rapidly heated the Multifunctional fermentation composite bacteria agent and the preparation method and application thereof of taste removal for livestock excrement composting | |
| CN101786911B (en) | Microorganism and enzyme powder composite biological agent for microbial organic fertilizer | |
| CN102992827B (en) | Active organic bacterial fertilizer and preparation method thereof | |
| CN109022327B (en) | Preparation method of microbial mixed inoculant and application of microbial mixed inoculant in high-temperature composting | |
| CN103497915A (en) | Complex microbial agent for composting fermentation and application thereof | |
| CN103896646B (en) | A kind of preparation method of microbial organic fertilizer | |
| CN102286376B (en) | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof | |
| CN106631551A (en) | Plant-growth-promoting bio-organic fertilizer and preparation method | |
| CN103980017A (en) | Method for rapidly producing high-quality organic fertilizer by virtue of high-temperature aerobic secondary fermentation | |
| CN101696393A (en) | Compound bacterium agent and method for accelerating fast maturity by inoculating compound bacterium agent to compost | |
| CN108863483A (en) | Quick animal excretion compost method | |
| CN108314585A (en) | Agriculture probiotics conversion stalk and the method for cultivating excrement production biological organic fertilizer | |
| CN102432355A (en) | Banana stem organic fertilizer and preparation method thereof | |
| CN107176891A (en) | It is a kind of to promote the biological agent and its production technology of stalk fast degradation | |
| CN113773987B (en) | Biological agent for improving aerobic fermentation efficiency of organic waste and preparation method thereof | |
| CN105255756A (en) | Deodorant complex microbial inoculant and preparation method and application thereof | |
| CN101817709A (en) | Biological organic fertilizer with disease preventing function and preparation method thereof | |
| CN109402013A (en) | A kind of high temperature resistant bacillus subtilis of yielding lipase and its application in compost | |
| CN106542926A (en) | A kind of Se-enriched microbial organic fertilizer with humic acid membrane wrapping and preparation method thereof | |
| CN103074228B (en) | Composite fermentation inoculant for processing animal wastes, and preparation method and application thereof | |
| CN109516873A (en) | Biogas residue fermenting decomposing agent and preparation method thereof | |
| CN109402014A (en) | A kind of bacillus of cellulase-producing and its application | |
| CN106748042B (en) | Biological organic fertilizer leavening and preparation method thereof | |
| CN102786327B (en) | Biological organic fertilizer produced from abamectin fermentation waste residue and preparation method thereof | |
| CN1266266C (en) | Microbial community and its preparation method and use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190219 |
|
| WW01 | Invention patent application withdrawn after publication |