CN109355189B - Method for removing protozoa during microalgae culture - Google Patents
Method for removing protozoa during microalgae culture Download PDFInfo
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- CN109355189B CN109355189B CN201811287668.3A CN201811287668A CN109355189B CN 109355189 B CN109355189 B CN 109355189B CN 201811287668 A CN201811287668 A CN 201811287668A CN 109355189 B CN109355189 B CN 109355189B
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Abstract
The invention relates to the technical field of microalgae cultivation, in particular to a method for removing protozoa during microalgae cultivation. The invention extracts water in the microalgae culture tank by (1) and introduces the water into a high-concentration gas dissolving device; (2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with high CO concentration; (3) the obtained microalgae culture water with high CO concentration is introduced into a microalgae culture tank, so that the effect of CO on fully inhibiting the respiratory enzyme of the protozoa is fully exerted, the protozoa die, the growth of the microalgae is fully promoted, the density of the microalgae is improved, the overall benefit of microalgae culture is improved, and the method for removing the protozoa during the microalgae culture can effectively remove the protozoa and cannot damage microalgae cells.
Description
Technical Field
The invention relates to the technical field of microalgae cultivation, in particular to a method for removing protozoa during microalgae cultivation.
Background
In the outdoor microalgae culture process, protozoa existing in the natural environment enter a culture solution through a water body and various culture operations, and the protozoa can be massively proliferated under the action of self-secreted organic matters released into the culture solution by the microalgae, so that the microalgae can not grow normally in severe cases.
At present, in order to kill protozoa in a culture solution, a physical filtration method and CO aeration are generally used 2 The method, hydrochloric acid acidification method, salinity increasing method, etc. have poor killing effect, so that 10-20PPM or less is added into cell sap in the process of culturing microalgae with low density (below OD 3)Hypochlorous acid or hydrogen peroxide and other oxidizing chemicals kill protozoa, but because hypochlorous acid or hydrogen peroxide have no oxidizing selectivity, microalgae cells are damaged while protozoa are killed by high-dose chemicals, and the yield of microalgae is reduced. Moreover, the effect of killing protozoa is also influenced by the concentration of algae cells and their secretions in the culture solution, the larger the cell concentration is, the more the secretions are, and since the algae cells and their secretions are all organic matter, they will react with the insecticide in the cell sap, so that the dosage of the insecticide acting on protozoa will also be reduced directly, thus reducing the killing power on protozoa.
Therefore, it is urgent to find a method for removing protozoa in culture of microalgae which can effectively remove protozoa without causing damage to microalgae cells.
Disclosure of Invention
In order to solve the above technical problems in the prior art, the present invention provides a method for removing protozoa during microalgae cultivation, which comprises the following steps:
a method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping water in the microalgae culture tank, and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with high CO concentration;
(3) and introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank.
Preferably, the microalgae culture water with high CO concentration is microalgae culture water with CO concentration more than or equal to 40 ppm. Further preferably, the microalgae culture water with high CO concentration is microalgae culture water with CO concentration more than or equal to 60 ppm. In a further preferred aspect, the microalgae culture water with high CO concentration is microalgae culture water with CO concentration of 80 ppm. When the CO concentration in the microalgae culture water is more than or equal to 40ppm, the action of the respiring enzyme of the protozoa can be better inhibited, so that the protozoa die; when the CO concentration in the microalgae culture water is more than or equal to 60ppm, the action of the respiring enzyme of the protozoa can be fully inhibited, so that all the protozoa die; when the concentration of CO in the microalgae culture water is 80ppm, the method can fully inhibit the action of respiration enzyme of the protozoa, so that all the protozoa die, fully promote the growth of the microalgae and improve the density of the microalgae.
Preferably, in the step (3), the obtained microalgae culture water with high CO concentration is introduced into a microalgae culture tank, and then is uniformly stirred to be fully diffused. The mixture is stirred evenly, so that the mixture is fully diffused, the whole CO concentration of the microalgae culture water can be rapidly improved, and protozoa can be rapidly killed.
Preferably, the steps (1), (2) and (3) are carried out cyclically at the same time. The steps (1), (2) and (3) are simultaneously and circularly carried out, so that the water level in the microalgae culture tank can be effectively kept stable, the fluctuation of the water level is reduced, the microalgae growth is facilitated, the microalgae density is favorably improved, the continuity is better, and the method is more suitable for industrialization.
Preferably, in the processes of the steps (1), (2) and (3), the CO concentration of culture water in the microalgae culture tank is controlled to be 60-80 ppm. At the moment, the action of the respiring enzymes of the protozoa can be fully inhibited, all the protozoa die, the growth of the microalgae can be fully promoted, and the density of the microalgae is improved.
Preferably, a filtration step is added in the processes of the steps (1), (2) and (3). Further preferably, the filtration is performed by using a filter screen with a mesh opening smaller than 60 μm. The filtering with the filter screen with the mesh less than 60 mu m can remove large protozoa in the water body, and further improve the speed and the effect of removing the protozoa.
Preferably, in the steps (1), (2) and (3), gas emitted from the microalgae culture water is collected. Carbon monoxide has a promoting effect on the growth of microalgae, but is toxic to higher plants and animals, and is diffused a little in the process of removing protozoa by using the carbon monoxide, and although the diffusion amount is small, the carbon monoxide is in a harmless state, but the carbon monoxide is still collected and removed or separated and then recycled. The results obtained by treating rotifers, protozoa, and E.coli with carbon monoxide were as follows:
insecticidal and bactericidal effects of CO
After 10 hours, most of protozoa and rotifers die, the pollution is reduced, the number of escherichia coli is greatly reduced, and the fertility of the escherichia coli is also deteriorated.
Compared with the prior art, the invention has the technical effects that:
the invention extracts water in the microalgae culture tank by (1) and introduces the water into a high-concentration gas dissolving device; (2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with high CO concentration; (3) the obtained microalgae culture water with high CO concentration is introduced into a microalgae culture tank, so that the effect of CO on fully inhibiting the respiratory enzyme of the protozoa is fully exerted, the protozoa die, the growth of the microalgae is fully promoted, the density of the microalgae is improved, the overall benefit of microalgae culture is improved, and the method for removing the protozoa during the microalgae culture can effectively remove the protozoa and cannot damage microalgae cells.
Drawings
FIG. 1 is a schematic of the process of the present invention.
FIG. 2 is a schematic of the process of the present invention.
Detailed Description
The technical solution of the present invention is further defined below with reference to the specific embodiments, but the scope of the claims is not limited to the description.
Example 1
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/4 water from the microalgae culture tank and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 60 ppm;
(3) and introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank.
Example 2
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/3 water from the microalgae culture tank, and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 40 ppm;
(3) and introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank.
Example 3
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/5 water from the microalgae culture tank and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water by using a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 80 ppm;
(3) introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank.
Example 4
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/4 water from the microalgae culture tank, and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 60 ppm;
(3) and introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank, and uniformly stirring to fully diffuse the microalgae culture water.
Example 5
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/4 water from the microalgae culture tank, and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 60 ppm;
(3) introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank.
And (3) performing the steps (1), (2) and (3) circularly at the same time.
Example 6
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/4 water from the microalgae culture tank and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 80 ppm;
(3) introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank.
In the processes of the steps (1), (2) and (3), the CO concentration of the culture water in the microalgae culture tank is controlled to be 60-80 ppm.
Example 7
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/4 water from the microalgae culture tank and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 60 ppm;
(3) and introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank.
Filtering by using a filter screen with 60 mu m meshes in the processes of the steps (1), (2) and (3).
Example 8
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/4 water from the microalgae culture tank, and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 60 ppm;
(3) introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank.
And (3) collecting gas emitted from the microalgae culture water in the steps (1), (2) and (3).
Example 9
A method for removing protozoa in microalgae culture comprises the following steps:
(1) pumping 1/4 water from the microalgae culture tank, and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water by using a high-concentration gas dissolving device to obtain microalgae culture water with the CO concentration of 80 ppm;
(3) and introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank, and uniformly stirring to fully diffuse the microalgae culture water.
In the processes of the steps (1), (2) and (3), the CO concentration of the culture water in the microalgae culture tank is controlled to be 60-80 ppm.
And (3) performing the steps (1), (2) and (3) circularly at the same time.
Filtering by using a filter screen with 60 mu m meshes in the processes of the steps (1), (2) and (3).
And (3) collecting gas emitted from the microalgae culture water in the steps (1), (2) and (3).
10 microalgae culture tanks of 30L are arranged, 20L liquid is respectively filled in the microalgae culture tanks, and microalgae are cultured until the density of the microalgae is kept stable, which is numbered as No. 1-10.
The results of using the methods of examples 1-9 with microalgae culture tanks 1-9 treated for 6h and No. 10 untreated as a control group and then compared are as follows:
therefore, the method can effectively kill the protozoa, fully promote the growth of the microalgae, improve the density of the microalgae and improve the overall benefit of microalgae culture.
Finally, it should be noted that the above embodiments are merely representative examples of the present invention. It is obvious that the technical solution of the present invention is not limited to the above embodiments, and many variations are possible. All modifications which can be derived or suggested by the person skilled in the art from the present disclosure are to be considered within the scope of the present invention.
Claims (4)
1. A method for removing protozoa in microalgae culture, which comprises the following steps:
(1) pumping water in the microalgae culture tank, and introducing the water into a high-concentration gas dissolving device;
(2) dissolving CO gas into the pumped microalgae culture water through a high-concentration gas dissolving device to obtain microalgae culture water with high CO concentration;
(3) introducing the obtained microalgae culture water with high CO concentration into a microalgae culture tank;
the microalgae culture water with high CO concentration is microalgae culture water with the CO concentration of 80 ppm;
in the step (3), the obtained microalgae culture water with high CO concentration is introduced into a microalgae culture tank and then is uniformly stirred to be fully diffused;
the steps (1), (2) and (3) are carried out circularly at the same time; and in the processes of the steps (1), (2) and (3), controlling the CO concentration of the culture water in the microalgae culture tank to be 80 ppm.
2. The method for removing protozoa in culturing microalgae according to claim 1, wherein a filtration step is added during the steps (1), (2), and (3).
3. The method for removing protozoa in culturing microalgae according to claim 2, wherein the filtering is performed by using a sieve having a mesh size of less than 60 μm.
4. The method for removing protozoa in microalgae cultivation as claimed in claim 1, wherein the gas released from microalgae cultivation water is collected during the steps (1), (2) and (3).
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382755A (en) * | 2011-07-28 | 2012-03-21 | 中国科学院青岛生物能源与过程研究所 | Microalgae culturing device and microalgae culturing method |
| CN104593263A (en) * | 2015-01-13 | 2015-05-06 | 新奥科技发展有限公司 | Method for treating protozoon pollution in microalgae culture |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382755A (en) * | 2011-07-28 | 2012-03-21 | 中国科学院青岛生物能源与过程研究所 | Microalgae culturing device and microalgae culturing method |
| CN104593263A (en) * | 2015-01-13 | 2015-05-06 | 新奥科技发展有限公司 | Method for treating protozoon pollution in microalgae culture |
Non-Patent Citations (3)
| Title |
|---|
| Cultivation of Freshwater Green Alga Neochloris oleoabundans in Non-Sterile Media Co-Inoculated with Protozoa;Licheng Peng,等;《THE CANADIAN JOURNAL OF CHEMICAL ENGINEERING》;20160331;第94卷;第439-445页 * |
| MASS CULTURE OF UNICELLULAR ALGAE USING THE "OPEN CIRCULATION METHOD";TAMOTSU KANAZAWA等;《J. Gen. Appl. Microbiol.》;19581231;第4卷(第3期);第135-152页 * |
| 单胞藻培养中原生动物的控制与清除;郭健等;《河北渔业》;20130430(第4期);第27-29页 * |
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