CN109355176A - A kind of cell separation equipment liquid channel system - Google Patents
A kind of cell separation equipment liquid channel system Download PDFInfo
- Publication number
- CN109355176A CN109355176A CN201811181654.3A CN201811181654A CN109355176A CN 109355176 A CN109355176 A CN 109355176A CN 201811181654 A CN201811181654 A CN 201811181654A CN 109355176 A CN109355176 A CN 109355176A
- Authority
- CN
- China
- Prior art keywords
- pinch valve
- channel system
- separation equipment
- cell separation
- liquid channel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
Abstract
The invention discloses a kind of cell separation equipment liquid channel systems, comprising: puncture needle, for detecting the photoelectric liquid level sensor and capacitive liquid level sensor of liquid condition, for monitoring the pressure sensor of pressure, pinch valve, cavity buffer structure and peristaltic pump；Wherein the lower plug of the cell filter punctures the first pipeline of access by puncture needle, first pipeline accesses the first pinch valve pipe by two-way joint, first pinch valve pipe is connect with the pinch valve, and the pinch valve, the pressure sensor and the peristaltic pump are connect with the cavity buffer structure；The photoelectric liquid level sensor and the capacitive liquid level sensor are arranged at the outside of first pipeline.Cell separation equipment liquid channel system of the present invention can achieve the purpose for improving complete machine liquid channel system Performance And Reliability.
Description
Technical field
The present invention relates to medical field extracorporeal diagnostic instrument technical fields, particularly relate to a kind of cell separation equipment liquid
Road system.
Background technique
Circulating tumor cell (circulating tumor cells, CTCs) refers to spontaneous or because operation of diagnosis and treatment is by entity
Tumor or transfer stove discharge into the tumour cell of Peripheral Circulation, are that malignant tumor patient postoperative recurrence and DISTANT METASTASES IN occurs
Major reason.In recent years, continuously improving with detection technique, CTCs detection is as a kind of novel non-invasive diagnostic work
Tool, early detection patient postoperative recurrence and DISTANT METASTASES IN, assessment curative effect in terms of application value have become clinic
The hot spot of research.
Since quantity of the CTCs in peripheral blood is few, need to usually be sought in about 100,000,000 leucocytes and 50,000,000,000 red blood cells
Only several tumour cells are looked for, therefore in order to improve the recall rate of CTCs, row CTCs need to usually be enriched with, i.e., will before detection
CTCs is separated from blood or other samples.The enrichment method of CTCs is broadly divided into immunomagnetic isolation by its principle at present
The concentration method of method and the physical property based on CTCs.Wherein, the concentration method of the physical property based on CTCs is mainly by density gradient
Centrifugal process and filtration method.
Filtration method is partition method (the isolation by size of epithelial based on tumour cell size
Tumor cells, ISET), tumour cell is mainly separated according to the of different sizes of tumour cell and normal cell.It is this to pass through
The cellular morphology of physical method for separation saves completely, and the various antigens or molecular labeling on surface do not influence cell without destruction
Characteristic provides good condition for subsequent detection.But the liquid channel system performance of existing equipment is bad, it can be in certain journey
Cell separating effect is influenced on degree.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of cell separation equipment liquid channel systems.
The technical scheme is that
A kind of cell separation equipment liquid channel system, connect with cell filter, comprising: puncture needle, it is liquid for detecting
The photoelectric liquid level sensor and capacitive liquid level sensor of state, for monitoring the pressure sensor of pressure, pinch valve, cavity buffering knot
Structure and peristaltic pump；Wherein the lower plug of the cell filter punctures the first pipeline of access by puncture needle, and the first pipeline passes through two
Pass joint accesses the first pinch valve pipe, and the first pinch valve pipe is connect with the pinch valve, the pinch valve, the pressure sensor
It is connect with the cavity buffer structure with the peristaltic pump；The photoelectric liquid level sensor and the capacitive liquid level sensor are equal
The outside of first pipeline is set.
Preferably, the cavity buffer structure includes the upper cover and lower cover being connected to each other, on the upper surface of the upper cover
It is provided with first interface, is provided with second interface at the side end face of the lower cover, is provided at the lower end surface of the lower cover
Third interface.
Preferably, the piping connection of the pressure sensor prevents liquid entry into the pressure to the first interface
The measurement accuracy of pressure sensor is influenced in sensor.
Preferably, the pipeline input port of the peristaltic pump is attached with the third interface, and it is slow to enter the cavity
The liquid for rushing structure will be influenced to be completely exhausted out in the third interface by gravity.
It preferably, further include three-way connection, three-way connection setting is in the pressure sensor and cavity buffering knot
Between structure.
It preferably, further include the second pinch valve pipe, described second pinch valve pipe one end is connect with the pinch valve, the other end
It is connect with the three-way connection.
Preferably, the upper cover and the lower cover are sealed by O-ring therebetween.
Preferably, the puncture needle is block side-port puncture needle.
By adopting the above technical scheme, the present invention include at least it is following the utility model has the advantages that
Cell separation equipment liquid channel system of the present invention can achieve and improve complete machine liquid channel system Performance And Reliability
Purpose.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of cell separation equipment liquid channel system of the present invention.
Wherein: 1. cell filters, 2. puncture needles, 3. tube joint assemblies, 4. photoelectric liquid level sensors, 5. capacitance liquid levels pass
Sensor, 6. first pipelines, 7. two-way joints, 8. first pinch valve pipes, 9. pinch valves, 10. second pinch valve pipes, 11. pressure pass
Sensor, 12. three-way connections, 13. cavity buffer structures, 131. upper covers, 132. lower covers, 133. first interfaces, 134. second interfaces,
135. third interface, 136. washers, 14. peristaltic pumps.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1, being connect to meet a kind of cell separation equipment liquid channel system of the invention with cell filter 1, wrap
It includes: puncture needle 2, for detecting the photoelectric liquid level sensor 4 and capacitive liquid level sensor 5 of liquid condition, for monitoring pressure
Pressure sensor 11, pinch valve 9, cavity buffer structure 13 and peristaltic pump 14；Wherein the lower plug of the cell filter 1 is by wearing
Pricker 2 punctures and accesses the first pipeline 6 by tube joint assembly 3, and the first pipeline 6 accesses the first pinch valve by two-way joint 7
Pipe 8, the first pinch valve pipe 8 are connect with the pinch valve 9, the pinch valve 9, the pressure sensor 11 and the peristaltic pump 14
It is connect with the cavity buffer structure 13；The photoelectric liquid level sensor 4 is arranged at institute with the capacitive liquid level sensor 5
State the outside of the first pipeline 6.
Preferably, the cavity buffer structure 13 includes the upper cover 131 being connected to each other and lower cover 132, in the upper cover 131
Upper surface on be provided with first interface 133, second interface 134 is provided at the side end face of the lower cover 132, under described
Third interface 135 is provided at the lower end surface of lid 132.The first interface 133, second interface 134 are set at third interface 135
It is equipped with washer 136.
Preferably, the piping connection of the pressure sensor 11 prevents liquid entry into described to the first interface 133
The measurement accuracy of pressure sensor 11 is influenced in pressure sensor 11.
Preferably, the pipeline input port of the peristaltic pump 14 is attached with the third interface 135, enters the chamber
The liquid of body buffer structure 13 will be influenced to be completely exhausted out in the third interface 135 by gravity.
It preferably, further include three-way connection 12, which is arranged in the pressure sensor 11 and the cavity
Between buffer structure 13.
It preferably, further include the second pinch valve pipe 10, described second pinch valve pipe, 10 one end is connect with the pinch valve 9,
The other end is connect with the three-way connection 12.
Preferably, the upper cover 131 and the lower cover 132 are sealed by O-ring therebetween.
Preferably, the puncture needle 2 is block side-port puncture needle 2, i.e., the inlet opening of puncture needle 2 is in the side of needle.
By cell filter 1 after gaging hole puncture needle 2 punctures the lower plug of the cell filter 1 equipped with liquid in the present embodiment
It is attached with liquid channel system, the second pinch valve pipe 10 is closed the first pinch valve pipe 8 and opened at this time, and peristaltic pump 14 starts to turn
Dynamic, liquid passes through 13 middle and lower part of cavity buffer structure after photoelectric liquid level sensor 4, capacitive liquid level sensor 5, pinch valve 9
Mouth enter cavity buffer structure 13 and be discharged by peristaltic pump 14, pressure sensor 11 connects first interface 133 and avoids feed liquor.Liquid
Body is monitored in pipeline by photoelectric liquid level sensor 4, capacitive liquid level sensor 5 and pressure sensor 11, when in cell filter 1
It can be detected by photoelectric liquid level sensor 4 and capacitive liquid level sensor 5 when emptying rate of liquid, when cell filter 1 blocks
Or pipeline can be detected by pressure sensor 11 when leaking, when the emptying of cell filter 1 or fluid path block the
One pinch valve pipe 8 opens the second pinch valve pipe 10 while closing, and air enters in cavity buffer structure 13, wriggles at this time
Pump 14, which continues to run, empties remaining liq in pipeline, completes the separation of a sample.
Cell separation equipment liquid channel system principle described in the present embodiment is as follows:
External diagnosis reagent and diagnostic sample-blood avoid band without in liquid channel system power source -14 pump head of peristaltic pump
The pump head cleaning problem and blood come easily condense in the problem in pump head and connector.External diagnosis reagent and diagnostic sample-blood
Without in liquid channel system control valve body, bring valve head cleaning problem and blood is avoided easily to condense in valve head and connector
Problem.
Using peristaltic pump 14 (squeezing silica gel hose by idler wheel pushes fluid to flow) control fluid in the fluid path pipeline into
Row continuous negative pressure extracts.
But the working principle of peristaltic pump 14 itself causes fluid to be that a pulse is wriggled in pipeline.Come up from microcosmic
It sees, there are the flow pulses rhythm of a fluid for the fluid of pipeline, and when will lead to extraction blood sample, blood sample is in pipeline
Imbalance of flow, there are pulse features.Particularly, the application that this filters tumour cell for us, blood sample pass through cell
When the particular filter film of filter 1, if there is no intermediate buffering cavity, there can be the fluid pulse play on microcosmic, it is not only right
There are certain damages for film, while tumour cell, when by film, there is also unstable pulses, will increase filtration cell not
Ballast.
When we are after negative pressure power source-peristaltic pump 14, cavity buffer structure 13 installs placement upward, works normally
State is that for top half there are certain air compartment, lower half portion is the state that fluid is full of in cavity buffer structure 13.When
When peristaltic pump 14 works, the negative pressure of fluid first passes around the air of 13 top of cavity buffer structure, has carried out a pulse
Release, this partial air will do it a preparatory buffering, by the negative pressure pressure of entire liquid channel system fluid switch to one it is flat
Along continuous uniform pressure, to reach control by cell filter 1 especially through microcosmic in filter membrane separation neoplastic process
On drawing liquid negative pressure it is balanced, to achieve the purpose that improve complete machine liquid channel system Performance And Reliability.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (8)
1. a kind of cell separation equipment liquid channel system, connect with cell filter characterized by comprising puncture needle, for examining
The photoelectric liquid level sensor and capacitive liquid level sensor for surveying liquid condition, for monitoring the pressure sensor of pressure, pinch valve, chamber
Body buffer structure and peristaltic pump；Wherein the lower plug of the cell filter punctures the first pipeline of access, the first pipe by puncture needle
The first pinch valve pipe is accessed by two-way joint in road, and the first pinch valve pipe is connect with the pinch valve, the pinch valve, the pressure
Force snesor and the peristaltic pump are connect with the cavity buffer structure；The photoelectric liquid level sensor and the capacitance liquid level
Sensor is arranged at the outside of first pipeline.
2. cell separation equipment liquid channel system as described in claim 1, it is characterised in that: the cavity buffer structure includes that
The upper cover and lower cover of this connection, are provided with first interface on the upper surface of the upper cover, set at the side end face of the lower cover
It is equipped with second interface, third interface is provided at the lower end surface of the lower cover.
3. cell separation equipment liquid channel system as claimed in claim 1 or 2, it is characterised in that: the pipe of the pressure sensor
Road is connected to the first interface, prevents liquid entry into the measurement essence that pressure sensor is influenced in the pressure sensor
Degree.
4. cell separation equipment liquid channel system a method according to any one of claims 1-3, it is characterised in that: the pipeline of the peristaltic pump
Input port is attached with the third interface, and the liquid for entering the cavity buffer structure will be influenced by gravity described
Third interface is completely exhausted out.
5. the cell separation equipment liquid channel system as described in claim 1-4 is any, it is characterised in that: it further include three-way connection,
The three-way connection is arranged between the pressure sensor and the cavity buffer structure.
6. cell separation equipment liquid channel system as claimed in claim 5, it is characterised in that: further include the second pinch valve pipe, institute
It states second pinch valve pipe one end to connect with the pinch valve, the other end is connect with the three-way connection.
7. cell separation equipment liquid channel system as claimed in claim 2, it is characterised in that: both the upper cover and the lower cover
Between pass through O-ring seal.
8. cell separation equipment liquid channel system as claimed in claim 1, it is characterised in that: the puncture needle is side opening
Puncture needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811181654.3A CN109355176A (en) | 2018-10-11 | 2018-10-11 | A kind of cell separation equipment liquid channel system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811181654.3A CN109355176A (en) | 2018-10-11 | 2018-10-11 | A kind of cell separation equipment liquid channel system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109355176A true CN109355176A (en) | 2019-02-19 |
Family
ID=65348634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811181654.3A Pending CN109355176A (en) | 2018-10-11 | 2018-10-11 | A kind of cell separation equipment liquid channel system |
Country Status (1)
| Country | Link |
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| CN (1) | CN109355176A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110687300A (en) * | 2019-09-30 | 2020-01-14 | 广州博鹭腾仪器仪表有限公司 | Full-automatic western blot processing device and processing system |
| CN114993581A (en) * | 2022-08-02 | 2022-09-02 | 深圳赛桥生物创新技术有限公司 | Pipeline air tightness detection device, method and system |
| WO2024016895A1 (en) * | 2022-07-22 | 2024-01-25 | 深圳赛桥生物创新技术有限公司 | Cell treatment device |
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Application publication date: 20190219 |