CN109350762A - A kind of medical dressing and preparation method thereof applied to chronic wound - Google Patents
A kind of medical dressing and preparation method thereof applied to chronic wound Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
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- A—HUMAN NECESSITIES
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- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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Abstract
The invention belongs to medical dressing fields, it is related to a kind of medical dressing applied to chronic wound, it includes bacteria cellulose, honey, chitosan and its derivative, pass through the compound mode of physics, honey and chitosan are formulated as antibacterial agent to be combined on bacteria cellulose film, or first pass through biological recombination process, honey and/or chitosan are added in the culture medium of bacteria cellulose film film forming procedure, it is prepared using physics complex method, 3 kinds of components are provided with good biocompatibility, pass through mutual synergistic effect, promote the healing of wound, avoid the formation of scar etc., it is with a wide range of applications.
Description
Technical field
The invention belongs to medical dressing fields, and in particular to a kind of applied to the medical dressing of chronic wound and its preparation side
Method.
Background technique
Chronic wounds refer to that wound cannot reach healing by normal healing process under the effect of various internal and external factors, into
Entering a kind of pathological inflammatory reactiveness causes wound prolonged refractory, needs the wound that could be healed by external force.Chronic wounds
The infection of staphylococcus aureus for often merging bacterium infection, especially penicillin resistant, is difficult to control wound infection, in addition wound
The factors such as necrotic tissue, poor, the tropic growth factors change of microcirculation are locally present, make chronic wounds is treated as problem.It removes
It treats outside primary disease, control wound infection and removing necrotic tissue are a steps important in chronic wound treatment.Whole body application
The therapy of antibiotic, often the effective range of prevention and treatment infection is not achieved in the drug concentration in wound part, in addition can also increase medicine
Agent amount increases side effect.The infection of wound can be sufficiently reduced using suitable local antibacterial dressing, while promoting wound comprehensively
Mouth recovery from illness.
Currently, the dressing of diversified load anti-microbial property is developed, can be mainly divided by its carrier form
The types such as fiber, hydrogel, sponge, film.Fibrous type antiseptic dressing can prevent bacterium infection, provide gas exchanges, still
It cannot largely keep liquid, and being easy to stick wound causes dressing painful；Hydrogel antimicrobial dressing is well combined with the surface of a wound, is easy to
Replacement, good water absorption, but mechanical performance is poor；Sponge-type antiseptic dressing absorbability is good, water vapo(u)r transmission is good, but it allows
Tissue grows into hole, is easy to bring secondary insult in dressing, and easily leave detritus in the surface of a wound；Film-type antiseptic dressing is transparent
Wound moist can be kept, but absorbent properties are poor.
On the other hand, in antibacterial agent selection, drug resistance is generated since antibiotic is easy induction body, antibacterial agent
Develop the hot spot become in recent years.Bacteria cellulose is the fiber with tridimensional nano net structure generated by microbial fermentation
Cellulosic material, it possesses numerous peculiar properties, such as extremely strong retentiveness, high polymerization degree and crystallinity, high wet tensile (strength), and
The advantages that good biocompatibility；Manuka honey is a kind of carbohydrate complex that ingredient is extremely complex, containing there are many antibacterials
Substance has and promotes wound healing, lymphocytosis, raising immunity, promotion endothelial progenitor cells is promoted to mobilize and accelerate the surface of a wound
The advantages that new vessels formation；Chitosan and its derivative has degradability in good biocompatibility and organism, also
With antibacterial, anti-inflammation hemostasia, reduce the surface of a wound exudation and promote wound tissue regeneration, repair, healing and other effects, and have flexibility,
The advantages that water imbibition and gas permeability.
103550817 A of patent application CN discloses a kind of bacteria cellulose/chitosan composite sponge dressing, by thin
The compound action of fungin and chitosan significantly improves its effect in terms of promoting wound healing, but the sponge is applied
It is also added with adhesive, hot melt adhesive or heat-sensitive glue in material, helps dressing positioning, the presence of the substances such as above-mentioned adhesive is for sensitivity
There are biggish security risks for skin；For another example 103480027 A of patent application CN discloses a kind of bacteria cellulose to meet shell poly-
Bacteria cellulose is dissolved in organic solvent in the patent by the preparation method of sugared fiber moist dressing, and chitosan is passed through quiet
The mode of Electrospun is compound on bacteria cellulose film, forming bacteria cellulose recombination chitosan moist dressing, but above-mentioned system
The higher cost of Preparation Method；It is compound solidifying that 103463124 A of patent application CN also discloses a kind of bacteria cellulose chitosan
Glue is made of chitosan, bacteria cellulose, antibacterial agent and thickener, wherein the antibacterial agent additionally added belongs to chemicals, it is long
The problem of phase use is easy to produce bacterial drug resistance.
It can be seen that it is at present that the compound research for being prepared into medical dressing of bacteria cellulose and chitosan is very more, still
It often adds some chemical reagent during the preparation process, limits its application, and therefore, how to select suitable natural material system
A kind of standby excellent antibacterial performance, biological property and the excellent medicine dressing of physicochemical property are the hot spots studied at present.
Summary of the invention
Based on the defect of the above-mentioned prior art, the present invention by selecting bacteria cellulose, Manuka honey and chitosan and
Raw material of its derivative as medical dressing is not added with any chemical assistant, and biocompatibility and application range are wider, and
It is compound with physics compound mode that Manuka honey and chitosan and its derivative is compound to thin by biology in preparation process
On fungin film, preparation process is simple, avoids the use of organic reagent, operates convenience and safety is higher.
To achieve the above object, the present invention provides a kind of medical dressing applied to chronic wound comprising bacterial fibers
Element, honey, chitosan and its derivative.
Further, the medical dressing is in parts by weight, comprising: 5-10 parts of bacteria celluloses, 0.5-5 parts of bees
Honey, 0.5-5 parts of chitosan and its derivatives.
Further, the medical dressing is in parts by weight, comprising: 6-9 parts of bacteria celluloses, 1-3 parts of honey,
1-3 parts of chitosan and its derivatives.
Further, the bacteria cellulose is by acetic acid Pseudomonas, Agrobacterium, rhizobium and Sarcina
One of or multiple-microorganism synthesize.
Further, the honey is Manuka honey.
Further, the chitosan and its derivative is selected from chitosan, carboxymethyl chitosan and chitosan quaternary ammonium salt
One of or it is several.
The present invention also provides a kind of preparation methods of the medical dressing of above-mentioned chronic wound, comprising the following steps:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium and is cultivated, bacteria cellulose film is obtained；
2) purification process of bacteria cellulose film: bacteria cellulose film prepared by step 1) is cleaned with purified water, then
After being put into lye passivation, cleaned with water for injection, the bacteria cellulose film purified；
3) physics is compound: honey, chitosan and its derivative being added in water for injection, filters, obtains antimicrobial；
It is compound that the bacteria cellulose film after purification that step 2) is obtained is added to progress physics in filtered antimicrobial；
4) drying of composite membrane: by the film of compound completion be dried to get.
Further, the preparation method of the medical dressing of the chronic wound, comprising the following steps:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium with the inoculum concentration of 1-20%, 28-35
DEG C stationary culture 5-14 days, obtain bacteria cellulose film；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into lye according to the ratio of membrane volume and lye volume 1:2
In, be warming up to 80-110 DEG C, taken out after stirring 1-12h, with water for injection clean bacteria cellulose film to its pH be 5.0-7.0,
And the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: accurately weighing Manuka honey, chitosan and its derivative is added in water for injection, carried out
Filter obtains the antimicrobial that concentration is 0.01%-10%；It is molten that antibacterial agent is added in the bacteria cellulose film of step 2) after purification
1h-12h is stirred in liquid；
4) drying of composite membrane: the film of step 3) is dried；
Further, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Further, the fermentation medium of the step 1) are as follows: glucose 25g/L, peptone 3g/L, yeast extract
5g/L, pH=4.0-6.0,121 DEG C of sterilizing 20min.
Further, lye is sodium hydroxide solution, concentration 0.01-2.0mol/L in the step 2).
Preferably, the concentration of lye is 0.5-1.0mol/L.
Further, filter type is selected from one of filter paper filtering and membrane filtration in the step 3).
Further, in the step 3) agitating mode in ultrasonic vibration, magnetic agitation, electric stirring one
Kind.
Further, combined temp is 10-80 DEG C in the step 3).
Preferably, combined temp is 30-40 DEG C in the step 3).
Further, the antimicrobial concentration being prepared in the step 3) is 3-7%.
Further, drying mode is selected from supercritical drying forming technique, vacuum drying molding skill in the step 4)
Art, freeze-drying one of forming technique and forced air drying forming technique.
The present invention also provides the preparation methods of the medical dressing of another above-mentioned chronic wound, comprising the following steps:
1) biology is compound: seed liquor being inoculated into fermentation medium, honey is added into fermentation medium and/or shell is poly-
It is cultivated after carbohydrates and their derivative, obtains composite bacterial cellulose film；
2) purification process of bacteria cellulose film: composite bacterial cellulose film prepared by step 1) is cleaned with purified water,
After being then placed in lye passivation, cleaned with water for injection, the composite bacterial cellulose film purified；
3) physics is compound: honey and/or chitosan and its derivative being added in water for injection, filters, obtains antibacterial agent
Solution；The composite bacterial cellulose film after purification that step 2) is obtained, which is added in filtered antimicrobial, carries out physics
It is compound；
4) drying of composite membrane: by the film of compound completion be dried to get.
Further, the preparation method of the medical dressing of above-mentioned chronic wound, comprising the following steps:
1) biology is compound: seed liquor being inoculated into fermentation medium with the inoculum concentration of 1-20%, and adds part wheat Lu
Card honey and/or chitosan and its derivative, 28-35 DEG C stationary culture 5-14 days, obtain composite bacterial cellulose film；
2) purification process of bacteria cellulose film: the composite bacterial cellulose film of step 1) is cleaned repeatedly with purified water,
To pH >=5.0 of composite bacterial cellulose film；Then according to membrane volume and the ratio of lye volume 1:2 by composite bacterial cellulose
Film is put into lye, is warming up to 80-110 DEG C, is taken out after stirring 1-12h, is cleaned composite bacterial cellulose film extremely with water for injection
Its pH is 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the composite bacterial cellulose purified
Film；
3) physics is compound: remaining Manuka honey and/or chitosan and its derivative are added in water for injection, into
Row filtering obtains the antimicrobial that concentration is 0.01%-10%；The composite bacterial cellulose film of step 2) after purification is added
1h-12h is stirred in antimicrobial；
4) drying of composite membrane: the film of step 3) is dried.
Further, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Further, the fermentation medium of the step 1) are as follows: glucose 25g/L, peptone 3g/L, yeast extract
5g/L, pH=5.0,121 DEG C of sterilizing 20min.
Further, lye is sodium hydroxide solution, concentration 0.01-2.0mol/L in the step 2).
Preferably, the concentration of lye is 0.5-1.0mol/L.
Further, filter type is selected from one of filter paper filtering and membrane filtration in the step 3).
Further, in the step 3) agitating mode in ultrasonic vibration, magnetic agitation, electric stirring one
Kind.
Further, combined temp is 10-80 DEG C in the step 3).
Preferably, combined temp is 30-40 DEG C in the step 3).
Further, the antimicrobial concentration being prepared in the step 3) is 3-7%.
Further, drying mode is selected from supercritical drying forming technique, vacuum drying molding skill in the step 4)
Art, freeze-drying one of forming technique and forced air drying forming technique.
The invention has the benefit that
(1) for non-animal immunological rejection will not occur for the bacteria cellulose that medical dressing of the present invention uses,
It with excellent biocompatibility, is contacted with living tissue without causing any toxicity or allergy side effect, not with tissue adhesion, is subtracted
Few cicatrization, water absorbing capacity also with super strength, the timely absorbing wound exudate of energy promote wound healing；Bacterial fibers
Element hole also rich in, is easy to fibroblastic growth, and wound tissue is promoted to repair；
Manuka honey, chitosan and its derivative are natural materials, nontoxic, have good bio-compatible
Property and broad-spectrum antibacterial；Therefore, the present invention is prepared using bacteria cellulose, Manuka honey, chitosan and its derivative
Medical dressing have good biocompatibility and safety；
Three kinds of component interactions can inhibit the effect of microorganism, more effectively keep away while promoting wound healing
Exempt from the formation of scar.
(2) medical dressing of the present invention is only made of bacteria cellulose, honey and chitosan and its derivative, is no longer additionally added
Add other chemical reagent, not only avoided the drug resistance of bacterium, but also reduces sensitive group and generate uncomfortable probability to chemical reagent,
Substantially increase the safety and application range of product.
(3) medical dressing preparation process of the present invention uses physics compound or the biological compound side in conjunction with physics compound phase
Formula, safe preparation process, simple, low manufacture cost, is suitble to be mass produced.
(4) product of the present invention clinical use is convenient, good can be adhered on wound, good water permeability, transparency is high, durable
Property is good, can reduce wound pain, accelerating wound healing, and dressing process will not cause secondary insult, be conducive to the surface of a wound and repair
It is multiple.
Specific embodiment
1 medical dressing of embodiment and its preparation
Medical dressing: 6 parts of bacteria celluloses, 2 parts of Manuka honeys, 2 parts of chitosans
Preparation method:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium with 10% inoculum concentration, 30 DEG C quiet
Culture 10 days is set, bacteria cellulose film is obtained；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into concentration according to the ratio of membrane volume and lye volume 1:2 is
In the NaOH solution of 1.0mol/L, be warming up to 100 DEG C, taken out after stirring 8h, with water for injection clean bacteria cellulose film to its
PH is 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: accurately weighing Manuka honey, chitosan is added in water for injection, carries out 0.22 μm of filter membrane mistake
Filter obtains the antimicrobial that concentration is 8%；Magnetic force in antimicrobial is added in the bacteria cellulose film of step 2) after purification
8h is stirred, combined temp is 25 DEG C；
4) drying of composite membrane: the film of step 3) is dried using vacuum drying forming technique；
5) step 4) dry products, both sides attach shaping membrane PET, the cut size 15.0cm*15.0cm after mold flattens,
It is sent after interior packet, outsourcing to sterilizing mechanism and carries out -60 ray sterilizing of cobalt, sterilizing dose 25kGy is packaged into final after sterilizing
Finished product.
Wherein, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Freeze-drying thallus employed in it is acetobacter xylinum, is preserved in China General Microbiological Culture Collection Center, and bacterial strain is compiled
Number be CGMCC 1.1812.
Wherein, the fermentation medium of the step 1) is glucose 25g/L, peptone 3g/L, yeast extract 5g/L, pH=
4.0-5.0 121 DEG C of sterilizing 20min.
2 medical dressing of embodiment and its preparation
Medical dressing: 7 parts of bacteria celluloses, 2 parts of Manuka honeys, 1 part of carboxymethyl chitosan
Preparation method:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium with 8% inoculum concentration, 28 DEG C of standings
Culture 10 days, obtains bacteria cellulose film；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into concentration according to the ratio of membrane volume and lye volume 1:2 is
In the NaOH solution of 0.5mol/L, 80 DEG C are warming up to, is taken out after stirring 4h, cleans bacteria cellulose film to its pH with water for injection
For 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: accurately weighing Manuka honey, carboxymethyl chitosan is added in water for injection, 0.22 μm of progress
Membrane filtration obtains the antimicrobial that concentration is 10%；It is molten that antibacterial agent is added in the bacteria cellulose film of step 2) after purification
Magnetic agitation 6h in liquid, combined temp are 40 DEG C；
4) drying of composite membrane: the film of step 3) is dried using forced air drying forming technique；
5) step 4) dry products, both sides attach shaping membrane PET, the cut size 15.0cm*15.0cm after mold flattens,
It is sent after interior packet, outsourcing to sterilizing mechanism and carries out -60 ray sterilizing of cobalt, sterilizing dose 25kGy is packaged into final after sterilizing
Finished product.
Wherein, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Freeze-drying thallus employed in it is acetobacter xylinum, is preserved in China General Microbiological Culture Collection Center, and bacterial strain is compiled
Number be CGMCC 1.1812.
Wherein, the fermentation medium of the step 1) is glucose 25g/L, peptone 3g/L, yeast extract 5g/L, pH=
4.0-5.0 121 DEG C of sterilizing 20min.
3 medical dressing of embodiment and its preparation
Medical dressing: 7 parts of bacteria celluloses, 1 part of Manuka honey, 2 parts of chitosan quaternary ammonium salts
Preparation method:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium with 12% inoculum concentration, 35 DEG C quiet
Culture 10 days is set, bacteria cellulose film is obtained；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into concentration according to the ratio of membrane volume and lye volume 1:2 is
In the NaOH solution of 0.2mol/L, 90 DEG C are warming up to, is taken out after stirring 6h, cleans bacteria cellulose film to its pH with water for injection
For 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: accurately weighing Manuka honey, chitosan quaternary ammonium salt is added in water for injection, 0.22 μm of progress
Membrane filtration obtains the antimicrobial that concentration is 0.01%；Antibacterial agent is added in the bacteria cellulose film of step 2) after purification
Magnetic agitation 4h in solution, combined temp are 60 DEG C；
4) drying of composite membrane: the film of step 3) is dried using freeze-drying forming technique；
5) step 4) dry products, both sides attach shaping membrane PET, the cut size 15.0cm*15.0cm after mold flattens,
It is sent after interior packet, outsourcing to sterilizing mechanism and carries out -60 ray sterilizing of cobalt, sterilizing dose 25kGy is packaged into final after sterilizing
Finished product.
Wherein, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Freeze-drying thallus employed in it is acetobacter xylinum, is preserved in China General Microbiological Culture Collection Center, and bacterial strain is compiled
Number be CGMCC 1.1812.
Wherein, the fermentation medium of the step 1) is glucose 25g/L, peptone 3g/L, yeast extract 5g/L, pH=
4.0-5.0 121 DEG C of sterilizing 20min.
4 medical dressing of embodiment and its preparation
Medical dressing: 6 parts of bacteria celluloses, 2 parts of Manuka honeys, 2 parts of chitosans
Preparation method:
1) biology is compound: seed liquor being inoculated into fermentation medium with 20% inoculum concentration, is added into fermentation medium
Add Manuka honey, additive amount 0.5%, 30 DEG C stationary culture 10 days, obtain composite bacterial cellulose film；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into concentration according to the ratio of membrane volume and lye volume 1:2 is
In the NaOH solution of 1.0mol/L, be warming up to 100 DEG C, taken out after stirring 8h, with water for injection clean bacteria cellulose film to its
PH is 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: accurately weighing chitosan and is added in water for injection, carries out 0.22 μm of membrane filtration, obtain concentration
For 8% antimicrobial；Magnetic agitation 8h in antimicrobial is added in the bacteria cellulose film of step 2) after purification, it is compound
Temperature is 25 DEG C；
4) drying of composite membrane: the film of step 3) is dried using vacuum drying forming technique；
5) step 4) dry products, both sides attach shaping membrane PET, the cut size 15.0cm*15.0cm after mold flattens,
It is sent after interior packet, outsourcing to sterilizing mechanism and carries out -60 ray sterilizing of cobalt, sterilizing dose 25kGy is packaged into final after sterilizing
Finished product.
Wherein, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Freeze-drying thallus employed in it is acetobacter xylinum, is preserved in China General Microbiological Culture Collection Center, and bacterial strain is compiled
Number be CGMCC 1.1812.
Wherein, the fermentation medium of the step 1) is glucose 25g/L, peptone 3g/L, yeast extract 5g/L, pH=
4.0-5.0 121 DEG C of sterilizing 20min.
5 medical dressing of embodiment and its preparation
Medical dressing: 7 parts of bacteria celluloses, 2 parts of Manuka honeys, 1 part of carboxymethyl chitosan
Preparation method:
1) biology is compound: seed liquor being inoculated into fermentation medium with 8% inoculum concentration, is added into fermentation medium
Carboxymethyl chitosan, additive amount 0.5%, 28 DEG C stationary culture 10 days, obtain composite bacterial cellulose film；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into concentration according to the ratio of membrane volume and lye volume 1:2 is
In the NaOH solution of 0.5mol/L, 80 DEG C are warming up to, is taken out after stirring 6h, cleans bacteria cellulose film to its pH with water for injection
For 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: accurately weighing Manuka honey and is added in water for injection, carries out 0.22 μm of membrane filtration, obtain
The antimicrobial that concentration is 10%；Magnetic agitation in antimicrobial is added in the bacteria cellulose film of step 2) after purification
6h, combined temp are 40 DEG C；
4) drying of composite membrane: the film of step 3) is dried using forced air drying forming technique；
5) step 4) dry products, both sides attach shaping membrane PET, the cut size 15.0cm*15.0cm after mold flattens,
It is sent after interior packet, outsourcing to sterilizing mechanism and carries out -60 ray sterilizing of cobalt, sterilizing dose 25kGy is packaged into final after sterilizing
Finished product.
Wherein, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Freeze-drying thallus employed in it is acetobacter xylinum, is preserved in China General Microbiological Culture Collection Center, and bacterial strain is compiled
Number be CGMCC 1.1812.
Wherein, the fermentation medium of the step 1) is glucose 25g/L, peptone 3g/L, yeast extract 5g/L, pH=
4.0-5.0 121 DEG C of sterilizing 20min.
6 medical dressing of embodiment and its preparation
Medical dressing: 7 parts of bacteria celluloses, 1 part of Manuka honey, 2 parts of chitosan quaternary ammonium salts
Preparation method:
1) biology is compound: seed liquor being inoculated into fermentation medium with 12% inoculum concentration, is added into fermentation medium
Add the Manuka honey and chitosan quaternary ammonium salt of half, additive amount 0.5%, 32 DEG C stationary culture 10 days, obtain composite bacterial fibre
Tie up plain film；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into concentration according to the ratio of membrane volume and lye volume 1:2 is
In the NaOH solution of 0.2mol/L, 90 DEG C are warming up to, is taken out after stirring 4h, cleans bacteria cellulose film to its pH with water for injection
For 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: remaining Manuka honey, chitosan quaternary ammonium salt being added in water for injection, carry out 0.22 μm
Membrane filtration obtains the antimicrobial that concentration is 0.01%；Antibacterial agent is added in the bacteria cellulose film of step 2) after purification
Magnetic agitation 4h in solution, combined temp are 60 DEG C；
4) drying of composite membrane: the film of step 3) is dried using freeze-drying forming technique；
5) step 4) dry products, both sides attach shaping membrane PET, the cut size 15.0cm*15.0cm after mold flattens,
It is sent after interior packet, outsourcing to sterilizing mechanism and carries out -60 ray sterilizing of cobalt, sterilizing dose 25kGy is packaged into final after sterilizing
Finished product.
Wherein, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Freeze-drying thallus employed in it is acetobacter xylinum, is preserved in China General Microbiological Culture Collection Center, and bacterial strain is compiled
Number be CGMCC 1.1812.
Wherein, the fermentation medium of the step 1) is glucose 25g/L, peptone 3g/L, yeast extract 5g/L, pH=
4.0-5.0 121 DEG C of sterilizing 20min.
Comparative example 1 is free of chitosan and its derivative medical dressing and its preparation
Medical dressing: 6 parts of bacteria celluloses, 2 parts of Manuka honeys
The preparation method is the same as that of Example 1, specifically:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium with 10% inoculum concentration, 30 DEG C quiet
Culture 10 days is set, bacteria cellulose film is obtained；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into concentration according to the ratio of membrane volume and lye volume 1:2 is
In the NaOH solution of 1.0mol/L, be warming up to 100 DEG C, taken out after stirring 8h, with water for injection clean bacteria cellulose film to its
PH is 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: accurately weighing Manuka honey and is added in water for injection, carries out 0.22 μm of membrane filtration, obtain
The antimicrobial that concentration is 8%；Magnetic agitation 8h in antimicrobial is added in the bacteria cellulose film of step 2) after purification,
Combined temp is 25 DEG C；
4) drying of composite membrane: the film of step 3) is dried using vacuum drying forming technique；
5) step 4) dry products, both sides attach shaping membrane PET, the cut size 15.0cm*15.0cm after mold flattens,
It is sent after interior packet, outsourcing to sterilizing mechanism and carries out -60 ray sterilizing of cobalt, sterilizing dose 25kGy is packaged into final after sterilizing
Finished product.
Wherein, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Freeze-drying thallus employed in it is acetobacter xylinum, is preserved in China General Microbiological Culture Collection Center, and bacterial strain is compiled
Number be CGMCC 1.1812.
Wherein, the fermentation medium of the step 1) is glucose 25g/L, peptone 3g/L, yeast extract 5g/L, pH=
4.0-5.0 121 DEG C of sterilizing 20min.
Medical dressing and its preparation of the comparative example 2 without honey
Medical dressing: 6 parts of bacteria celluloses, 2 parts of chitosans
The preparation method is the same as that of Example 1, specifically:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium with 10% inoculum concentration, 30 DEG C quiet
Culture 10 days is set, bacteria cellulose film is obtained；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until thin
PH >=5.0 of fungin film；Then bacteria cellulose film is put into concentration according to the ratio of membrane volume and lye volume 1:2 is
In the NaOH solution of 1.0mol/L, be warming up to 100 DEG C, taken out after stirring 8h, with water for injection clean bacteria cellulose film to its
PH is 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: chitosan and its derivative is added in water for injection, is carried out 0.22 μm of membrane filtration, is obtained dense
The antimicrobial that degree is 8%；Magnetic agitation 8h in antimicrobial is added in the bacteria cellulose film of step 2) after purification, it is multiple
Closing temperature is 25 DEG C；
4) drying of composite membrane: the film of step 3) is dried using forced air drying forming technique；
5) step 4) dry products, both sides attach shaping membrane PET, the cut size 15.0cm*15.0cm after mold flattens,
It is sent after interior packet, outsourcing to sterilizing mechanism and carries out -60 ray sterilizing of cobalt, sterilizing dose 25kGy is packaged into final after sterilizing
Finished product.
Wherein, the seed liquor process for preparation of the step 1) are as follows:
1. rejuvenation of spawn: freeze-drying thallus being inoculated in slant medium under aseptic condition, 36h is cultivated at 30 DEG C, repeats 2
It is secondary；Wherein, the slant medium composition are as follows: glucose 10%, yeast powder 1%, calcium carbonate 2%, agar 1.8%；
2. strain expands culture: the strain of picking inclined-plane culture is into aseptic culture fluid, and at 30 DEG C, 150r/min concussion is trained
It supports for 24 hours；Wherein culture solution group becomes glucose 25g/L, peptone 3g/L, yeast extract 5g/L.
Freeze-drying thallus employed in it is acetobacter xylinum, is preserved in China General Microbiological Culture Collection Center, and bacterial strain is compiled
Number be CGMCC 1.1812.
Wherein, the fermentation medium of the step 1) is glucose 25g/L, peptone 3g/L, yeast extract 5g/L, pH=
4.0-5.0 121 DEG C of sterilizing 20min.
The test of the different medical dressing absorbent properties of experimental example 1
Test object: the antibacterial repair materials of embodiment 1-6, comparative example 1-2 preparation.
Test philosophy: establishing criteria YY/T 0471.1-2004 contact Wound dressing test method part 1: liquid is inhaled
The property received has carried out water suction experiment.
Specific test process is as follows:
Above-mentioned dressing is respectively cut into 5cm × 5cm size, 10 every kind；
Test preparation solution A: weighing 8.298g sodium chloride and 0.368g calcium chloride dihydrate powder respectively, with the volumetric flask of 1L
Constant volume to obtain the final product.
It is respectively placed in the sample of above-mentioned 5cm × 5cm specification in culture dish, the examination for being preheated to (37 ± 1) DEG C is then added
Solution is tested, additional amount is 40 times or so of sample.It moves into drying box, keeps 30min at (37 ± 1) DEG C；It is clamped with tweezers
One jiao of sample, dangle 30s after weigh.
This operation is repeated to other 9 samples, is averaged the absorption multiple up to sample, absorbs that multiple is bigger to illustrate sample
The absorbability of product is better.
1 medical dressing of table absorbs multiple result
| Project | Absorb multiple |
| Embodiment 1 | 20.1 |
| Embodiment 2 | 18.9 |
| Embodiment 3 | 19.8 |
| Embodiment 4 | 20.5 |
| Embodiment 5 | 21.3 |
| Embodiment 6 | 19.5 |
| Comparative example 1 | 15.6 |
| Comparative example 2 | 1.5 |
By table 1, statistics indicate that, dressing provided by the invention has good absorbent properties, is conducive to help affected part extensive as early as possible
It is multiple, wherein can all make the absorbent properties of dressing aobvious if lacking any one component in honey or chitosan and its derivative
Write decline.
The different medical dressing cytotoxicity tests of experimental example 2
Test object: the antibacterial medical dressing and commercially available silver-ion antibiotic dressing of embodiment 1-6, comparative example 1-2 preparation
(more nation's antibacterial medical dressing 5.5*7.5cm self-adhering-type).
Test philosophy: establishing criteria " the 5th part cell in vitro poison of GB 16886.5-2017 BiologicalEvaluationofMedicalDevice
Property test ", carried out cytotoxicity experiment.
2 cytotoxicity criterion of table
| Viable cell survival rate (%) | Meaning |
| ≥70 | No cytotoxicity |
| <70 | Potential cytotoxicity |
3 anti-biotic material cytotoxicity of table
By cytotoxicity test result it is found that medical dressing of the present invention and commercially available silver-ion antibiotic dressing phase
Than there is better cell Proliferation effect, biocompatibility is more preferable, clinically causes the probability of adverse reaction smaller, product
Clinical use is safer.
The different medical dressing bacteriostasis property tests of experimental example 3
Test object: the antibacterial repair materials of embodiment 1-6, comparative example 1-2 preparation, commercially available silver-ion antibiotic dressing.
Test philosophy: establishing criteria GB/T 20944.1-2007 antibacterial textile performance evaluation part 1: agar plate
Diffusion method has carried out anti-microbial property experiment.
Specific test process is as follows:
1. principle: injecting two layers of agar medium in plate, lower layer is aseptic culture medium, and upper layer is inoculation medium.Examination
Sample is placed on two layers of culture medium, and culture is after a certain period of time, qualitative to comment according to the degree of bacterial reproduction at culture medium and sample contacts
Determine the anti-microbial property of sample.
2. testing bacterium
Use gram-positive bacteria --- staphylococcus aureus and Gram-negative bacteria --- Escherichia coli.
3. the preparation of test bacteria liquid
3.1 are gone bail for oese deposits bacterium, is inoculated on agar culture plate with method of scoring, cultivates at 37 DEG C ± 2 DEG C
24h。
3.2 take nutrient broth 20ml to be put into the triangular flask of 100ml, take the colonies typical on 3.1 plates to connect with oese
Kind is cultivated in meat soup, condition of culture are as follows: and 37 DEG C ± 2 DEG C of temperature, vibration frequency 110min-1, time 18h-24h.
3.3 use 20 times of dilution nutrient broths of distilled water, and adjusting the bacterial concentration after culture with it is 1 × 108CFU/ml-5
×108CFU/ml, as test bacteria liquid.Bacterial concentration is measured using spectrophotometer or method appropriate.
4. the preparation of sample
4.1 sample
Representational sample is chosen from embodiment 1-6 sample, every kind of bacterium tests 4 pieces of (2 pieces of front, 2 pieces of reverse side) circles
Shape sample, diameter 25mm ± 5mm.Sample should not sterilize.
4.2 control sample
For comparative example 1-2 sample as control sample, size is identical as sample.
5. step
5.1 prepare lower layer's aseptic culture medium.10.0mL agar medium is poured into sterilized petri dishes, and makes its solidification.
5.2 prepare upper layer inoculation medium.It takes 45 DEG C ± 2 DEG C of agar medium 150.0mL to be put into beaker, 1mL is added
Test bacteria liquid.Concussion beaker keeps Multiple drug resistance uniform, pours into 5.0mL into each plate in 5.1, and make its condensation.
Sample and control sample are put in plate center with aseptic nipper by 5.3 respectively, are equably pressed on agar medium,
Until being contacted well between sample and agar medium.
After 5.4 are placed on sample on agar medium, it is immediately placed in 37 DEG C ± 2 DEG C of incubator and cultivates 18h-24h,
Ensure that sample and agar medium are kept in contact in entire culture period.
6. the calculating and evaluation of result
6.1 each samples at least measure at 3, and the antibacterial bandwidth of sample is calculated as follows.
H=(D-d)/2
In formula:
H --- antibacterial bandwidth, unit are millimeter (mm)；
D --- the antibacterial average value with outer diameter, unit are millimeter (mm)
D --- specimen finish, unit are millimeter (mm).
After the 6.2 antibacterial bands of measurement, sample is removed from agar medium with tweezers, with being connect below microexamination sample
Touch the food supplements in region.
6.3, according to the presence or absence of bacterial reproduction and the case where antibacterial band, the antibacterial effect of each sample are evaluated by table 1.
The evaluation of 4 antibacterial effect of table
6.4 when all samples are all satisfied in table 1 requirement of " effect is good ", then it is assumed that the sample has antibacterial effect.
The different medical dressing antibacterial effects of table 5
By biocidal property testing result it is found that antibacterial repair materials of the present invention are compared with commercially available silver-ion antibiotic dressing,
With same fungistatic effect, but compared to comparative example 1, promoted in the significant effect of antibacterial aspect.
The content measuring of the different medical dressing unit area functional components of experimental example 4
Test object: the antibacterial medical dressing of embodiment 1-6, comparative example 1-2 preparation.
Test philosophy: it is examined according to the detection method of sucrose and maltose in first honey of " Chinese Pharmacopoeia " version in 2015
The content for surveying sucrose and maltose, the content of honey is gone out according to Conversion Calculation.Using elemental analyser, measure respectively chitosan,
Nitrogen content in carboxymethyl chitosan, chitosan quaternary ammonium salt, pure BC film and composite material, afterwards using formula calculate chitosan,
Carboxymethyl chitosan and the chitosan quaternary ammonium salt content in composite membrane.
Specific test process is as follows:
(1) it sucrose and maltose: is measured by the following method with high performance liquid chromatography, calculates separately content.
Chromatographic condition and system suitability: using Prevail Carbohyrate ES as chromatographic column, with acetonitrile-water
(75:25) is mobile phase；Differential refraction detector detection.Number of theoretical plate is calculated by fructose peak should be not less than 2000.
The preparation of standard curve: precision weighs fructose reference substance 1.0g, grape reference substance 0.8g respectively, sets same tool plug cone
In shape bottle, 40% acetonitrile 20ml is added in precision, and dissolution shakes up, as fructose, glucose control product stock solution.Another precision weighs
Control sucrose product 0.2g, maltose reference substance 0.2g, sets in same stuffed conical flask, precision plus people 4 0% acetonitrile 10ml, molten
Solution, shakes up, as sucrose, maltose reference substance stock solution.It is accurate respectively to measure fructose, glucose control product stock solution and sugarcane
Sugar, maltose reference substance stock solution add 40% acetonitrile to be made into the fructose, glucose, sucrose, maltose mixing control of various concentration
Product solution.During each strength solution is prepared, the dosage and dilution volume of stock solution be see the table below.
Precision draws each 15 μ l of mixed reference substance solution, injects liquid chromatograph, measures respectively.It is cross with reference substance concentration
Coordinate draws standard curve using peak area value as ordinate, calculates regression equation.
The preparation of test solution: taking this product about lg, accurately weighed, sets in stuffed conical flask, precision plus 4 0% acetonitrile of people
20ml, dissolution, shake up, filter, take subsequent filtrate to get.
Measuring method: precision measures test solution 15ul, injects liquid chromatograph, and measurement is contained by calibration curve method calculating
Amount.
(2) measurement of chitosan, carboxymethyl chitosan and chitosan quaternary ammonium salt content: Germany Elmentar Vario is utilized
EL type III elemental analyser tests out chitosan, carboxymethyl chitosan, chitosan quaternary ammonium salt, pure BC film and composite wood respectively
Nitrogen content in material is calculated by formula below:
Chitosan (carboxymethyl chitosan, chitosan quaternary ammonium salt) (%)=(WC-WBC)/Wch×100
WCFor the nitrogen content in composite material, WBCFor the nitrogen content in pure BC film, Wch is chitosan, carboxymethyl chitosan
Or the nitrogen content in chitosan quaternary ammonium salt.
Test result see the table below
The content of the different medical dressing unit area functional components of table 6
Note :/represent and this substance is not detected.
Upper table statistics indicate that, the dressing being prepared by biological mixed binding physics mode, functional component content is bright
The aobvious effect component content compound higher than simple physics.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, the embodiment not to
The scope of the patents of the invention is limited, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (10)
1. a kind of medical dressing applied to chronic wound, which is characterized in that including bacteria cellulose, honey, chitosan and its
Derivative.
2. the medical dressing according to claim 1 applied to chronic wound, which is characterized in that the medical dressing according to
Parts by weight meter, including 5-10 parts of bacteria celluloses, 0.5-5 parts of honey, 0.5-5 parts of chitosan and its derivatives.
3. the medical dressing according to claim 2 applied to chronic wound, which is characterized in that the medical dressing according to
Parts by weight meter, including 6-9 parts of bacteria celluloses, 1-3 parts of honey, 1-3 parts of chitosan and its derivatives.
4. the medical dressing according to claim 1 applied to chronic wound, which is characterized in that the honey is Mai Luka
Honey.
5. the medical dressing according to claim 1 applied to chronic wound, which is characterized in that the chitosan and its spread out
Biology is selected from one of chitosan, carboxymethyl chitosan and chitosan quaternary ammonium salt or several.
6. a kind of preparation method of any one of claim 1-5 medical dressing of the chronic wound, comprising the following steps:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium and is cultivated, bacteria cellulose film is obtained；
2) purification process of bacteria cellulose film: bacteria cellulose film prepared by step 1) is cleaned with purified water, is then placed in
After lye passivation, cleaned with water for injection, the bacteria cellulose film purified；
3) physics is compound: honey, chitosan and its derivative being added in water for injection, filters, obtains antimicrobial；It will step
It is compound that the rapid bacteria cellulose film after purification 2) obtained is added to progress physics in filtered antimicrobial；
4) drying of composite membrane: by the film of compound completion be dried to get.
7. the preparation method of the medical dressing of chronic wound according to claim 6, comprising the following steps:
1) preparation of bacteria cellulose film: seed liquor is inoculated into fermentation medium with the inoculum concentration of 1-20%, 28-35 DEG C quiet
Culture 5-14 days is set, bacteria cellulose film is obtained；
2) purification process of bacteria cellulose film: the bacteria cellulose film of step 1) is cleaned repeatedly with purified water, until bacterium is fine
Tie up pH >=5.0 of plain film；Then bacteria cellulose film is put into lye according to the ratio of membrane volume and lye volume 1:2, is risen
Temperature to 80-110 DEG C, stir 1-12h after take out, with water for injection clean bacteria cellulose film to its pH be 5.0-7.0, and carefully
The endotoxic content of bacterium is less than or equal to 0.03EU/ml, the bacteria cellulose film purified；
3) physics is compound: Manuka honey, chitosan and its derivative being added in water for injection, are filtered, is obtained dense
Degree is the antimicrobial of 0.01%-10%；The bacteria cellulose film of step 2) after purification is added in antimicrobial and is stirred
1h-12h；
4) drying of composite membrane: the film of step 3) is dried.
8. a kind of preparation method of any one of claim 1-5 medical dressing of the chronic wound, comprising the following steps:
1) biology is compound: seed liquor is inoculated into fermentation medium, added into fermentation medium honey and/or chitosan and
It is cultivated after its derivative, obtains composite bacterial cellulose film；
2) purification process of bacteria cellulose film: composite bacterial cellulose film prepared by step 1) is cleaned with purified water, then
After being put into lye passivation, cleaned with water for injection, the composite bacterial cellulose film purified；
3) physics is compound: remaining honey and/or chitosan and its derivative being added in water for injection, filters, obtains antibacterial
Agent solution；The composite bacterial cellulose film after purification that step 2) is obtained, which is added in filtered antimicrobial, carries out object
It manages compound；
4) drying of composite membrane: by the film of compound completion be dried to get.
9. the preparation method of the medical dressing of chronic wound according to claim 8, comprising the following steps:
1) biology it is compound: seed liquor is inoculated into fermentation medium with the inoculum concentration of 1-20%, and add Manuka honey and/
Or chitosan and its derivative, 28-35 DEG C stationary culture 5-14 days, obtain composite bacterial cellulose film；
2) purification process of bacteria cellulose film: the composite bacterial cellulose film of step 1) is cleaned repeatedly with purified water, until multiple
Close pH >=5.0 of bacteria cellulose film；Then composite bacterial cellulose film is put according to the ratio of membrane volume and lye volume 1:2
Enter in lye, be warming up to 80-110 DEG C, is taken out after stirring 1-12h, clean composite bacterial cellulose film to its pH with water for injection
For 5.0-7.0, and the content of bacterial endotoxin is less than or equal to 0.03EU/ml, the composite bacterial cellulose film purified；
3) physics is compound: remaining Manuka honey and/or chitosan and its derivative being added in water for injection, carried out
Filter obtains the antimicrobial that concentration is 0.01%-10%；Antibacterial is added in the composite bacterial cellulose film of step 2) after purification
1h-12h is stirred in agent solution；
4) drying of composite membrane: the film of step 3) is dried.
10. any one of any one of the claim 1-5 medical dressing or claim 6-9 preparation method is used in preparation
Application in the medical dressing of chronic wound.
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| CN113117128A (en) * | 2019-12-30 | 2021-07-16 | 华东交通大学 | Homogeneous multifunctional bacterial cellulose-based skin dressing and preparation method thereof |
| CN113318129A (en) * | 2021-06-24 | 2021-08-31 | 中国人民大学 | Efficient antibacterial honey paste and preparation method thereof |
| WO2021236024A1 (en) * | 2020-05-21 | 2021-11-25 | Ptt Public Company Limited | Preparation of biocellulose wound dressing comprising honey as an antimicrobial active ingredient |
| CN114469840A (en) * | 2022-03-21 | 2022-05-13 | 英中再生医学(山东)有限公司 | Product with scald relieving and oxidation resisting capabilities and preparation method and application thereof |
| CN116926146A (en) * | 2023-07-19 | 2023-10-24 | 湖南爱杰特生物科技有限公司 | Bacterial cellulose and preparation method and application thereof |
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| WO2021236024A1 (en) * | 2020-05-21 | 2021-11-25 | Ptt Public Company Limited | Preparation of biocellulose wound dressing comprising honey as an antimicrobial active ingredient |
| CN113318129A (en) * | 2021-06-24 | 2021-08-31 | 中国人民大学 | Efficient antibacterial honey paste and preparation method thereof |
| CN114469840A (en) * | 2022-03-21 | 2022-05-13 | 英中再生医学(山东)有限公司 | Product with scald relieving and oxidation resisting capabilities and preparation method and application thereof |
| CN116926146A (en) * | 2023-07-19 | 2023-10-24 | 湖南爱杰特生物科技有限公司 | Bacterial cellulose and preparation method and application thereof |
| CN116926146B (en) * | 2023-07-19 | 2024-03-12 | 湖南爱杰特生物科技有限公司 | Bacterial cellulose and preparation method and application thereof |
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