CN109342342A - A method of utilizing carbon quantum dot Visual retrieval L-cysteine concentration - Google Patents
A method of utilizing carbon quantum dot Visual retrieval L-cysteine concentration Download PDFInfo
- Publication number
- CN109342342A CN109342342A CN201811408460.2A CN201811408460A CN109342342A CN 109342342 A CN109342342 A CN 109342342A CN 201811408460 A CN201811408460 A CN 201811408460A CN 109342342 A CN109342342 A CN 109342342A
- Authority
- CN
- China
- Prior art keywords
- solution
- quantum dot
- cysteine
- concentration
- carbon quantum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The invention discloses a kind of method using carbon quantum dot Visual retrieval L-cysteine concentration, using in a linear relationship between the concentration of L-cysteine in mixed solution and the absorbance of mixed solution, to detect the concentration of L-cysteine；The mixed solution contains TMB, hydrogen peroxide, carbon quantum dot, phosphate citrate buffer and phosphate buffer.Carbon quantum dot is used for the concentration of Visual retrieval L-cysteine by the present invention, and detection process is simple and convenient, high sensitivity, detection limit it is low, it can be achieved that in actual sample L-cysteine quick detection.The detection method testing cost is low, has great application value, wide market.In addition, the Water-soluble carbon quantum dot solution based on chicken blood preparation, stability is good, easy to operate, and preparation is simple, small toxicity, is environmentally protective non-toxic product.
Description
Technical field
The present invention relates to technical field of medical chemistry, utilize carbon quantum dot Visual retrieval L- more particularly, to a kind of
The method of semicystinol concentration.
Background technique
L-cysteine is also known as cysteine, is a kind of human body nonessential amino acid.L-cysteine is a kind of with life
The amino acid for managing function is in more than 20 kinds of amino acid of constitutive protein matter uniquely with the ammonia of reproducibility group sulfydryl (- SH)
Base acid.L-cysteine is present in keratin, and keratin is the main protein for constituting nail, toenail, skin and hair.L-
Cysteine can help collagen tissue to generate, and can maintain the elasticity and skin texture of skin.It is also related with the protein of digestive ferment, and half
Cystine can many important enzymes in general-SH base donor, such as succinate dehydrogenase, lactic dehydrogenase.L-cysteine master
It applies in cosmetics, medicine, field of food.In terms of cosmetics, it is used to prepare hair-waving essence, suncream, hair tonic perfume and supports
Send out essence etc..Field of medicaments be used to prepare methyl cysteine, ethyl cystein, acetylcysteine, acthiol-J,
The drugs such as Ethitanin and comprehensive amino acid preparation.Cysteine can be used as the protection medicine of radiation damage.In food
Aspect, L-cysteine be used as bread fermentation auxiliary agent (ripener), milk powder and fruit juice antioxidant stabilizer and dote on poultry food
The nutritional agents etc. of object.The detection of L-cysteine concentration is of great significance.
Prior art CN104777117A discloses a kind of detection method of cysteine, and still, the reagent of this method is high
It is expensive, testing cost is higher, it is difficult to the large-scale application in social production activity.It is badly in need of developing a kind of new half Guang ammonia of detection L-
The method of acid concentration.
Summary of the invention
The present invention is to overcome preparation process described in the above-mentioned prior art complicated, at high cost, and stability deficiency defect mentions
For a kind of method using carbon quantum dot Visual retrieval L-cysteine concentration, the detection method high sensitivity provided, detection
It limits low, can be realized the quick visualization detection of L-cysteine concentration in sample, and reagent is cheap, testing cost is low, tool
There are great application value, wide market.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
A method of using carbon quantum dot Visual retrieval L-cysteine concentration, utilizing L-cysteine in mixed solution
It is in a linear relationship between concentration and the absorbance of mixed solution, to detect the concentration of L-cysteine；
The mixed solution contains TMB, hydrogen peroxide, carbon quantum dot, phosphate citrate buffer and phosphate buffer.
The present invention creatively has found that carbon quantum dot can be used in the concentration of Visual retrieval L-cysteine, detection process
It is simple and convenient, high sensitivity, detection limit it is low, it can be achieved that in actual sample L-cysteine quick detection.Carbon quantum dot is stablized
Property it is good, preparation cost is low, the detection method testing cost is low, have great application value, wide market.
Preferably, the carbon quantum dot uses Water-soluble carbon quantum dot solution, and the Water-soluble carbon quantum dot solution is by chicken
Blood is prepared.Water-soluble carbon quantum dot solution, stability is good, and preparation is simple, easy to operate, small toxicity.
Preferably, described method includes following steps:
S1. Water-soluble carbon quantum dot solution is prepared；
S2. the normal concentration solution for preparing detection solution and L-cysteine, the detection solution is mixed with normal concentration solution
Conjunction obtains mixed solution, establishes the linear pass of the concentration of L-cysteine in the absorbance and the mixed solution of mixed solution
System；The detection solution contains TMB, hydrogen peroxide, Water-soluble carbon quantum dot, phosphate citrate buffer, phosphate buffer
And water；
S3. solution to be measured and the detection solution are mixed to get mixed solution to be measured, test the suction of the mixed solution to be measured
Luminosity, to obtain the concentration of L-cysteine；The volume of the mixed solution to be measured is equal with the mixed solution, it is described to
The volume for surveying solution to be measured in mixed solution is equal with the mixed solution Plays strength solution.
The step of preparing Water-soluble carbon quantum dot solution is as follows:
New fresh poultry blood is collected, is centrifuged 15 minutes under conditions of 10000 rpm, 4 DEG C, upper serum is removed, collects lower layer's blood clotting
Block.Blood clot is squeezed out into moisture on filter paper, then blood clot after extruding is transferred to baking oven, is dried at 40 ~ 60 DEG C, so
It pulverizes on mortar afterwards.Chicken blood powder in mass ratio 0.95% ~ 1.15% is soluble in water, then carried out at 170 ~ 190 DEG C
Sufficiently reaction is reacted 11 ~ 13 hours, and 0.45 μm of filtering with microporous membrane of reaction solution is removed insoluble impurities after room temperature is cooling
Acquire Water-soluble carbon quantum dot solution.
Preferably, the hydrogen peroxide is 30% hydrogenperoxide steam generator.
Preferably, the concentration of the TMB solution is 10 mM.
Preferably, the concentration of the Water-soluble carbon quantum dot solution is 10 mg/mL.
Preferably, the detection solution is by TMB solution, 30% hydrogen peroxide, Water-soluble carbon quantum dot solution, phosphate citrate
40: 1: 125: 500: 875: 460 preparations obtain by volume for acid buffer, phosphate buffer and water.
The normal concentration solution is a series of solution of L-cysteines containing various concentration.Concentration can be
1000.00、500.00、250.00、125.00、62.50、31.25、15.63、7.81、3.90、1.95、0.98、0.48、0.24、
0.12、0 μM。
Preferably, the volume ratio of the normal concentration solution and detection solution is 1: 4.
Preferably, the volume ratio of the solution to be measured and detection solution is 1: 4.
Preferably, it after the mixed solution to be measured reacts 30 min under the conditions of 37 DEG C, is terminated and is reacted with sulfuric acid.It is described
The concentration of sulfuric acid can be 2M.
Moreover, the study found that above-mentioned detection solution can be used in the specific detection of L-cysteine.
Specific step is as follows for specific detection:
M1. compound concentration be 500 μM L-Phenylalanine, DL-Tryptophan, L-Methionine, Glycine,
Glucose,creatinine,sucrose,L-Serine,L-Cysteine；
M2. detection solution is prepared, the mixeding liquid volume ratio for detecting solution is TMB solution: 30% hydrogen peroxide: carbon quantum dot is molten
Liquid: phosphate citrate buffer: phosphate buffer: water=40: 1: 125: 500: 875: 460；
M3. take L-Phenylalanine, DL-Tryptophan of same volume, L-Methionine, Glycine,
Glucose, creatinine, sucrose, L-Serine, L-Cysteine, as measurement solution, into each measurement solution
The detection solution of 4 times of measurement liquor capacities is added；
M4. after 37 DEG C of 30 min of incubation, 2 M sulfuric acid is added and terminate reaction；
M5. it is each added to the absorbance of the measurement solution of detection solution in 450 nm detection, establishes the absorbance of measurement solution
With the relationship of measurement solution composition.
When testing absorbance, the excitation wavelength used can be 450 nm.
In the present invention, TMB refers to 3,3', 5,5'- tetramethyl benzidines.
Compared with prior art, the beneficial effects of the present invention are:
Carbon quantum dot is used for the concentration of Visual retrieval L-cysteine by the present invention, and detection process is simple and convenient, high sensitivity,
Detection limit it is low, it can be achieved that in actual sample L-cysteine quick detection.The detection method testing cost is low, has great
Application value, wide market.
In addition, the Water-soluble carbon quantum dot solution based on chicken blood preparation, stability is good, easy to operate, and preparation is simple, toxicity
It is small, it is environmentally protective non-toxic product.
Detailed description of the invention
Fig. 1 is the canonical plotting of various concentration L-cysteine standard solution obtained in embodiment 1.
Fig. 2 is that detection solution schemes the specificity of detection L-cysteine in embodiment 1.
Specific embodiment
The present invention is further illustrated With reference to embodiment.
Raw material in embodiment can be by being commercially available；
Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Embodiment 1
A method of utilizing carbon quantum dot Visual retrieval L-cysteine concentration, the specific steps are as follows:
Step 1: Water-soluble carbon quantum dot solution is prepared based on chicken blood, specifically:
New fresh poultry blood is collected, is centrifuged 15 minutes under conditions of 10000 rpm, 4 DEG C, upper serum is removed, collects lower layer's blood clotting
Block.Blood clot is squeezed out into moisture on filter paper, then blood clot after extruding is transferred to baking oven, is dried at 40 ~ 60 DEG C, so
It pulverizes on mortar afterwards.Chicken blood powder in mass ratio 1.0% is soluble in water, it is then sufficiently reacted at 180 DEG C, instead
It answers 12 hours, 0.45 μm of filtering with microporous membrane removal insoluble impurities of reaction solution is acquired into water solubility after room temperature is cooling
Carbon quantum dot solution.The concentration of Water-soluble carbon quantum dot solution is 10 mg/mL.
Step 2: the absorbance intensity of mixed solution and the linear relationship of L-cysteine concentration are established, specifically:
A, compound concentration be 1000.00,500.00,250.00,125.00,62.50,31.25,15.63,7.82,3.90,
1.95,0.98,0.48,0.24,0.12,0 μM of L-cysteine solution, as standard solution；
B, detection solution is prepared, it is TMB solution, 30% hydrogen peroxide, carbon quantum dot solution, phosphorus that detection solution, which is by detection solution,
Sour lime acid buffer, phosphate buffer, water mixed liquor, volume ratio 40:1:125:500:875:460；TMB solution
Concentration be 10 mM.
C, it takes the standard solution of 40 μ L to be placed in enzyme mark hole, the detection solution of 160 μ L is added into each enzyme mark hole, obtains
To mixed solution, finally making the total volume of the mixed solution in each enzyme mark hole is 200 μ L, the standard in each enzyme mark hole
The volume ratio of solution and detection solution is 1:4；
D, after 37 DEG C of 30 min of incubation, 2 M sulfuric acid is added and terminate reaction；
E, the absorbance of the mixed solution of detection solution is each added in 450 nm detection, with concentration of standard solution for horizontal seat
Mark, using absorbance as ordinate, and carries out linear fit, establishes the line of the absorbance of mixed solution and the concentration of L-cysteine
Sexual intercourse.
The linear relationship of the concentration of the absorbance and L-cysteine of mixed solution is as shown in Figure 1, linear equation are as follows: and Y=
0.73728X-0.00315, R2=0.996。
Step 3: the specificity of detection L-cysteine is detected on the basis of linear relationship, specifically:
A, compound concentration be 500 μM L-Phenylalanine, DL-Tryptophan, L-Methionine, Glycine,
Glucose,creatinine,sucrose,L-Serine,L-Cysteine；
B, prepare detection solution, detect solution mixeding liquid volume ratio be TMB solution, 30% hydrogen peroxide, carbon quantum dot solution,
Phosphate citrate buffer, phosphate buffer, water=40:1:125:500:875:460；
C, take L-Phenylalanine, DL-Tryptophan of 40 μ L, L-Methionine, Glycine, Glucose,
Creatinine, sucrose, L-Serine, L-Cysteine are placed in enzyme mark hole, as measurement solution, to each enzyme mark hole
The middle detection solution that 160 μ L are added, finally making the total volume of the solution in each enzyme mark hole is 200 μ L, each enzyme mark hole
In measurement solution and detect solution volume ratio be 1:4；
D, after 37 DEG C of 30 min of incubation, 2 M sulfuric acid is added and terminate reaction；
E, 450 nm detection each be added to detection solution measurement solution light absorption value, establish measurement solution light absorption value with
Measure the relationship of solution composition.
Measure solution light absorption value and detection solution relationship as shown in Fig. 2, measure solution have to L-Cysteine it is higher
Light absorption value, and to L-Phenylalanine, DL-Tryptophan, L-Methionine, Glycine, Glucose,
The light absorption value of creatinine, sucrose, L-Serine are lower, and it is higher to illustrate that this method has the detection of L-cysteine
Specificity.
Step 4: detection solution is added into the solution to be measured of L-cysteine, mixed solution to be measured is obtained, is detected to be measured
The light absorption value intensity of mixed solution determines the concentration of L-cysteine in solution to be measured according to the linear relationship that step 2 is established.
Specifically according to the following steps:
A, detection solution is prepared, the mixeding liquid volume ratio for detecting solution is TMB solution: 30% hydrogen peroxide: carbon quantum dot solution:
Phosphate citrate buffer: phosphate buffer: water=40:1:125:500:875:460；
B, take the solution to be measured of 40 μ L to be placed in enzyme mark hole, the detection solution of 160 μ L be added into each enzyme mark hole, obtain to
Mixed solution is surveyed, finally makes the total volume of the mixed solution to be measured in each enzyme mark hole for 200 μ L, in each enzyme mark hole
The volume ratio of solution to be measured and detection solution is 1:4；
D, after 37 DEG C of 30 min of incubation, 2 M sulfuric acid is added and terminate reaction；
E, the light absorption value of the mixed solution to be measured of detection solution, the line established according to step 2 are each added in 450 nm detection
Property equation, determines the concentration of L-cysteine in solution to be measured.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention.For those of ordinary skill in the art, may be used also on the basis of the above description
To make other variations or changes in different ways.There is no necessity and possibility to exhaust all the enbodiments.It is all this
Made any modifications, equivalent replacements, and improvements etc., should be included in the claims in the present invention within the spirit and principle of invention
Protection scope within.
Claims (9)
1. a kind of method using carbon quantum dot Visual retrieval L-cysteine concentration, which is characterized in that utilize mixed solution
It is in a linear relationship between the concentration of middle L-cysteine and the absorbance of mixed solution, to detect the concentration of L-cysteine；
The mixed solution contains TMB, hydrogen peroxide, carbon quantum dot, phosphate citrate buffer and phosphate buffer.
2. the method according to claim 1, wherein the carbon quantum dot use Water-soluble carbon quantum dot solution,
The Water-soluble carbon quantum dot solution is prepared by chicken blood.
3. method according to claim 1 or 2, which is characterized in that described method includes following steps:
S1. Water-soluble carbon quantum dot solution is prepared；
S2. the normal concentration solution for preparing detection solution and L-cysteine, the detection solution is mixed with normal concentration solution
Conjunction obtains mixed solution, establishes the linear pass of the concentration of L-cysteine in the absorbance and the mixed solution of mixed solution
System；The detection solution contains TMB, hydrogen peroxide, Water-soluble carbon quantum dot, phosphate citrate buffer, phosphate buffer
And water；
S3. solution to be measured and the detection solution are mixed to get mixed solution to be measured, test the suction of the mixed solution to be measured
Luminosity, to obtain the concentration of L-cysteine；The volume of the mixed solution to be measured is equal with the mixed solution, it is described to
The volume for surveying solution to be measured in mixed solution is equal with the mixed solution Plays strength solution.
4. according to the method described in claim 3, it is characterized in that, the hydrogen peroxide is 30% hydrogenperoxide steam generator.
5. according to the method described in claim 3, it is characterized in that, the concentration of the TMB solution is 10 mM.
6. according to the method described in claim 3, it is characterized in that, the concentration of the Water-soluble carbon quantum dot solution is 10 mg/
mL。
7. according to the method described in claim 3, it is characterized in that, the detection solution is by TMB solution, 30% hydrogen peroxide, water
Dissolubility carbon quantum dot solution, phosphate citrate buffer, phosphate buffer and water by volume 40: 1: 125: 500: 875:
460 preparations obtain.
8. according to the method described in claim 3, it is characterized in that, the volume ratio of the solution to be measured and detection solution is 1: 4.
9. according to the method described in claim 3, it is characterized in that, the mixed solution to be measured reacts 30 under the conditions of 37 DEG C
After min, is terminated and reacted with sulfuric acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811408460.2A CN109342342A (en) | 2018-11-23 | 2018-11-23 | A method of utilizing carbon quantum dot Visual retrieval L-cysteine concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811408460.2A CN109342342A (en) | 2018-11-23 | 2018-11-23 | A method of utilizing carbon quantum dot Visual retrieval L-cysteine concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109342342A true CN109342342A (en) | 2019-02-15 |
Family
ID=65317706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811408460.2A Pending CN109342342A (en) | 2018-11-23 | 2018-11-23 | A method of utilizing carbon quantum dot Visual retrieval L-cysteine concentration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109342342A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111100636A (en) * | 2019-12-25 | 2020-05-05 | 太原师范学院 | Styrene cyanine dye functionalized carbon dot sensor and preparation method and application thereof |
| CN112630179A (en) * | 2020-12-09 | 2021-04-09 | 安徽师范大学 | Prussian blue quantum dot with oxide mimic enzyme property, preparation method thereof and method for detecting L-cysteine |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603611A (en) * | 2017-09-17 | 2018-01-19 | 西南大学 | One kind has Catalyzed Synthesis By Peroxidase active fluoro carbon quantum dot and preparation method thereof |
| CN107748143A (en) * | 2017-10-30 | 2018-03-02 | 吉林大学 | A kind of hydrogen peroxide colorimetric sensing method based on fluorescent polymer analogue enztme |
| CN107764788A (en) * | 2017-09-26 | 2018-03-06 | 徐州工程学院 | A kind of synthetic method of carbon quantum dot, carbon quantum dot and detection Fe3+Method |
| CN110231297A (en) * | 2019-06-14 | 2019-09-13 | 西北师范大学 | The method for visualizing measurement content of hydrogen peroxide using doping type carbon quantum dot |
-
2018
- 2018-11-23 CN CN201811408460.2A patent/CN109342342A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603611A (en) * | 2017-09-17 | 2018-01-19 | 西南大学 | One kind has Catalyzed Synthesis By Peroxidase active fluoro carbon quantum dot and preparation method thereof |
| CN107764788A (en) * | 2017-09-26 | 2018-03-06 | 徐州工程学院 | A kind of synthetic method of carbon quantum dot, carbon quantum dot and detection Fe3+Method |
| CN107748143A (en) * | 2017-10-30 | 2018-03-02 | 吉林大学 | A kind of hydrogen peroxide colorimetric sensing method based on fluorescent polymer analogue enztme |
| CN110231297A (en) * | 2019-06-14 | 2019-09-13 | 西北师范大学 | The method for visualizing measurement content of hydrogen peroxide using doping type carbon quantum dot |
Non-Patent Citations (2)
| Title |
|---|
| QINGMEI ZHONG 等: "Synthesis of catalytically active carbon quantum dots and its application for colorimetric detection of glutathione", 《SENSORS & ACTUATORS: B. CHEMICAL》 * |
| ZHONGHUA XUE等: "A colorimetric sensor of cysteine based on self-assembly nanostructures of Fe3t-H2O2/Tetramethylbenzidine system with "On-Off" switching function", 《ANALYTICAL BIOCHEMISTRY》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111100636A (en) * | 2019-12-25 | 2020-05-05 | 太原师范学院 | Styrene cyanine dye functionalized carbon dot sensor and preparation method and application thereof |
| CN111100636B (en) * | 2019-12-25 | 2022-06-24 | 太原师范学院 | Styrene cyanine dye functionalized carbon dot sensor and preparation method and application thereof |
| CN112630179A (en) * | 2020-12-09 | 2021-04-09 | 安徽师范大学 | Prussian blue quantum dot with oxide mimic enzyme property, preparation method thereof and method for detecting L-cysteine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102124337B (en) | Device for rapid determination of disease-associated thiol compounds | |
| TWI357978B (en) | In-vitro diagnostic medical devices for determinin | |
| Feigl et al. | Normal arterial blood pH, oxygen, and carbon dioxide tensions in unanesthetized dogs | |
| Campanella et al. | New biosensor for superoxide radical used to evidence molecules of biomedical and pharmaceutical interest having radical scavenging properties | |
| CN102864208B (en) | Quality control product of AV/BV combined test kit and preparation method thereof | |
| CN109342342A (en) | A method of utilizing carbon quantum dot Visual retrieval L-cysteine concentration | |
| CN109350557A (en) | Anti-apolexis composition, skin care item comprising NADH and ceramide and the preparation method and application thereof | |
| Greenstein et al. | Chemical studies on human cancer. I. Cytochrome oxidase, cytochrome c, and copper in normal and neoplastic tissues | |
| CN102292641A (en) | Lyophilised dabigatran | |
| Matzeu et al. | Functionalized Mouth‐Conformable Interfaces for pH Evaluation of the Oral Cavity | |
| CN109374709A (en) | Based on metal-organic framework material-ferroheme electrochemical sensor preparation method and its usage | |
| Campanella et al. | Comparison of fluorimetric, voltammetric and biosensor methods for the determination of total antioxidant capacity of drug products containing acetylsalicylic acid | |
| CN109781719A (en) | A kind of kit detecting trypsase and its inhibitor based on platinum cluster | |
| CN109342341A (en) | A method of utilizing carbon quantum dot Visual retrieval concentration of hydrogen peroxide | |
| Frizzell et al. | Oxidative metabolism of rabbit ileal mucosa | |
| AU2018100445A4 (en) | A method for dopamine detection with GO-PtCu nano-enzyme | |
| CN105445468A (en) | Phenylalanine detection kit | |
| CN108593633A (en) | A kind of Test paper for quickly detecting saliva uric acid | |
| CN107897170A (en) | A kind of buccal swab DNA preservation solution and preparation method thereof | |
| CN111088312A (en) | Helicobacter pylori detection kit and preparation method thereof | |
| CN105510583A (en) | Kit used for detecting enzyme activity of biotin enzyme | |
| CN105372218A (en) | Method for detecting enzymatic activity of biotinidase | |
| CN115628958A (en) | Egg powder matrix standard sample containing rimantadine and preparation method thereof | |
| Campanella et al. | An enzyme sensor for determination of acetylcholine and choline in rat brain extracts | |
| CN111419822B (en) | Calcium butyrate microcapsule and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190215 |
|
| RJ01 | Rejection of invention patent application after publication |