CN109337805A - A kind of biochip hybridizing device - Google Patents
A kind of biochip hybridizing device Download PDFInfo
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- CN109337805A CN109337805A CN201811136092.0A CN201811136092A CN109337805A CN 109337805 A CN109337805 A CN 109337805A CN 201811136092 A CN201811136092 A CN 201811136092A CN 109337805 A CN109337805 A CN 109337805A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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Abstract
The present invention relates to a kind of biochip hybridizing devices, include from the bottom to top successively magnetic material layer that layer is set, to hybridization hybrid chip substrate layer, layer of silica gel and the interior substrate layer equipped with magnetic material, one surface of the substrate layer is equipped with fixing groove, the layer of silica gel is protruded into the fixing groove by a surface of layer of silica gel, and the layer of silica gel through-thickness is partially submerged into the fixing groove；Another surface of the layer of silica gel is equipped at least one subregion for being mutually mutually separated and sealing, and the subregion of at least one being arranged in layer of silica gel is corresponded with the detection sample of at least one on the point sample face to hybridization hybrid chip substrate layer respectively.Biochip hybridizing device of the invention can solve the problems, such as that chip because of multiple subregions causes cross contamination in the prior art, can get good hybridization sealing effect.
Description
Technical field
The present invention relates to Measurement for Biotechnique, in particular to a kind of biochip hybridizing device.
Background technique
Chip in the prior art generally uses conventional slide, and size about 75mm*25mm*1mm hybridizes drop in life
The probe region of object chip is pressed on hybridization solution with tabletting (coverslip), prevents hybridization solution from overflowing, then will have hybridization solution and tabletting
Chip is put into hybridizing box, and hybridizing box surrounding presses upper box cover sealing, moisturizing with adhesive tape.
The brilliant core microarray hybridization box of the rich biology research and development difficult to understand in Beijing is that biochip hybridizing box is more representative existing
There is technology (shown in Figure 1, or referring to network address http://science.capitalbio.com/hcysj/swxphc/
10708.shtml)。
When needing to detect more than one sample on a chip, chip can be with subregion, each area small pressure appropriate
Piece pushes down hybridization solution, then will have the chip of hybridization solution and tabletting to be put into hybridizing box, and hybridizing box surrounding presses upper box cover close with adhesive tape
Envelope moisturizing (it is shown in Figure 2, or referring to network address http://science.capitalbio.com/upload/Image/
mrtp/107304419.png)。
The hybrid device can effectively prevent reaction solution to volatilize, and dust in air is avoided to pollute, be various micro-array chips into
The ideal auxiliary tool of row hybridization or immune response.
But said chip tabletting hybrid device needs to use tabletting, tabletting hybridization is needed with the higher hybridization solution (first of viscosity
Amide hybridization solution), poor fluidity；Further, since worrying that hybridization solution is overflowed from tabletting generates cross contamination, not can be carried out relatively acutely
Concussion mix.The result that these limitations all can cause hybridization reaction insufficient.
It is overflowed in addition, hybridization solution is not present in said chip tabletting hybrid device in the case where only one subregion from tabletting
The case where generating cross contamination, but there is hybridization solution in the case where more than one area and overflow generation cross contamination from tabletting
The more situation of risk, especially subregion, the risk that hybridization solution overflows generation cross contamination from tabletting are bigger.
Summary of the invention
The technical problems to be solved by the present invention are: providing a kind of biochip hybridizing device, can solve in the prior art
The problem of chip causes cross contamination because of multiple subregions, can get good hybridization sealing effect.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention are as follows:
A kind of biochip hybridizing device, include from the bottom to top successively magnetic material layer that layer is set, to hybridization hybrid chip substrate
Layer, layer of silica gel and the interior substrate layer equipped with magnetic material, the no sample to hybridization hybrid chip substrate layer is facing towards the magnetic material
Layer, the point sample to hybridization hybrid chip substrate layer is facing towards the layer of silica gel；One surface of the substrate layer is equipped with fixing groove, the silicon
Glue-line is protruded into the fixing groove by a surface of layer of silica gel, and the layer of silica gel through-thickness is partially submerged into the fixation
Slot；Another surface of the layer of silica gel is equipped at least one subregion for being mutually mutually separated and sealing, at least one be arranged in layer of silica gel
A subregion is corresponded with the detection sample of at least one on the point sample face to hybridization hybrid chip substrate layer respectively；The magnetism
Material layer and the interior substrate layer magnetic absorption equipped with magnetic material.
The beneficial effects of the present invention are:
The layer of silica gel for designing the above-mentioned subregion for being mutually mutually separated and sealing equipped at least one, for corresponding to chip test sample to be checked
This subregion, specifically, layer of silica gel can be equipped at least two subregions, for corresponding to multiple subregions of the multiple samples to be detected of chip
(integral sealing and subregion sealing) or layer of silica gel can also be provided only with a subregion, for corresponding to one sample to be detected of chip
Subregion (integral sealing)；In the fixing groove of the laminated insertion substrate layer of silica gel, it is in close contact with fixing groove；Layer of silica gel another side
It is in close contact with chip point sample face.It is mixed with magnetic material (such as superparamagnetic magnetic material) in substrate layer, magnetic material layer is set
In chip no sample face, magnetic material layer attracts substrate layer, layer of silica gel two sides stress, and then realizes perfect sealing.
Detailed description of the invention
Fig. 1 is the side structure decomposition diagram of the biochip hybridizing device of the embodiment of the present invention；
Fig. 2 is side structure schematic diagram of the biochip hybridizing device of the embodiment of the present invention after assembling；
Fig. 3 is the main view in the biochip hybridizing device of the embodiment of the present invention to hybridization hybrid chip substrate layer；
Fig. 4 is the main view in the biochip hybridizing device of the embodiment of the present invention after hybridization hybrid chip substrate layer point sample；
Fig. 5 is the main view of layer of silica gel in the biochip hybridizing device of the embodiment of the present invention；
Fig. 6 is the main view on a surface of substrate layer in the biochip hybridizing device of the embodiment of the present invention；
Fig. 7 is the main view on another surface of substrate layer in the biochip hybridizing device of the embodiment of the present invention；
Fig. 8 is the main view of magnetic material layer in the biochip hybridizing device of the embodiment of the present invention.
Label declaration:
1, magnetic material layer；2, to hybridization hybrid chip substrate layer；21, at least one on the point sample face of hybridization hybrid chip substrate layer
A detection sample；3, layer of silica gel；31, the subregion of at least one being arranged in layer of silica gel；4, substrate layer；41, it is set on substrate layer
The subregion of at least one set.
Specific embodiment
To explain the technical content, the achieved purpose and the effect of the present invention in detail, below in conjunction with embodiment and cooperate attached
Figure is explained.
The most critical design of the present invention is: designing above-mentioned layer of silica gel for corresponding to chip subregion, magnetic material layer is utilized
It is acted on the magnetic absorption of the interior substrate layer for setting (mixed) magnetic material, realizes perfect sealing.
Please refer to Fig. 1-8, a kind of biochip hybridizing device, include from the bottom to top magnetic material layer 1 that successively layer is set, to
Hybridization hybrid chip substrate layer 2, layer of silica gel 3 and the interior substrate layer 4 for setting (or mixed) magnetic material, the nothing to hybridization hybrid chip substrate layer 2
Specimen face side is to the magnetic material layer 1, and the point sample to hybridization hybrid chip substrate layer 2 is facing towards the layer of silica gel 3；The substrate layer
4 surface is equipped with fixing groove, and the layer of silica gel 3 is protruded into the fixing groove by a surface of layer of silica gel, and the layer of silica gel 3
Through-thickness is partially submerged into the fixing groove；Another surface of the layer of silica gel 3 is equipped at least one and is mutually mutually separated and seals
Subregion, the subregion 31 of at least one being arranged in layer of silica gel respectively at least one on the point sample face to hybridization hybrid chip substrate layer
A detection sample 21 corresponds；The magnetic material layer 1 and interior 4 magnetic absorption of substrate layer equipped with magnetic material.
As can be seen from the above description, the beneficial effects of the present invention are:
The above-mentioned layer of silica gel for being equipped with the subregion that at least two are mutually mutually separated and seal is designed, it is multiple to be checked for corresponding to chip
Multiple subregions (integral sealing and subregion sealing) of test sample sheet.Or the layer of silica gel of only one subregion, for corresponding to chip one
The subregion (integral sealing) of a sample to be detected；In the fixing groove of the laminated insertion substrate layer of silica gel, it is in close contact with fixing groove；
Layer of silica gel another side and chip point sample face are in close contact.It is mixed in substrate layer magnetic material (such as superparamagnetic magnetic material), it will
Magnetic material layer is set to chip no sample face, and magnetic material layer attracts substrate layer, layer of silica gel two sides stress, and then realizes perfect close
Envelope.
Further, the magnetic material layer 1 is correspondingly arranged with the substrate layer 4.
Further, the number for the subregion being arranged in layer of silica gel is 1-24.
Further, the material of the substrate layer 4 is plastics.
Further, the substrate layer 4 the preparation method comprises the following steps: be added into the plastic cement for injection molding using magnetic material, infuse
Modeling prepares the substrate layer that magnetic material is equipped in described.
Further, a surface of the substrate layer 4 is equipped at least two subregions for being mutually mutually separated and sealing, substrate layer
The subregion 41 of at least one of upper setting is arranged in a one-to-one correspondence with the subregion 31 of at least one being arranged in layer of silica gel respectively.
Please refer to Fig. 1-8, the embodiment of the present invention one are as follows:
The biochip hybridizing device of the present embodiment includes magnetic material layer 1, core to be hybridized that successively layer is set from the bottom to top
Piece substrate layer 2, layer of silica gel 3 and the interior substrate layer 4 equipped with magnetic material.It is magnetic in above-mentioned hierarchical structure from size specification
Material layer 1, to hybridization hybrid chip substrate layer 2 and substrate layer 4 length and width it is in the same size, the length of layer of silica gel 3 is less than magnetic material layer
1, to hybridization hybrid chip substrate layer 2 and substrate layer 4, the width of layer of silica gel 3 is slightly less than magnetic material layer 1, to hybridization hybrid chip substrate layer 2
With substrate layer 4.No sample to hybridization hybrid chip substrate layer 2 is facing towards the magnetic material layer 1, to hybridization hybrid chip substrate layer 2
Point sample is facing towards the layer of silica gel 3；One surface of the substrate layer 4 be equipped with fixing groove, the layer of silica gel 3 by layer of silica gel a table
Face is protruded into the fixing groove, and 3 through-thickness of the layer of silica gel is partially submerged into the fixing groove；The layer of silica gel 3 it is another
One surface is equipped with 10 subregions for being mutually mutually separated and sealing, and 10 subregions being arranged in layer of silica gel are respectively and to hybridization hybrid chip substrate
10 detection samples on the point sample face of layer correspond；The magnetic material layer 1 and the interior substrate layer 4 equipped with magnetic material
Magnetic absorption.
The present embodiment separates each hybridization region using substrate layer and layer of silica gel, most layer of silica gel insertion substrate layers
Fixing groove is in close contact to seal, and the layer of silica gel of fraction contacts pressurization with to hybridization hybrid chip substrate layer.The present embodiment can
Plastic cement for injection molding is mixed into using appropriate superparamagnetic or other magnetic materials, injection molding production obtains the interior base for being equipped with magnetic material
Material layer；Substrate layer has the fixing groove designed for layer of silica gel insertion, and the substrate layer of magnetic material is equipped in layer of silica gel insertion；It is embedding
There is the substrate layer of layer of silica gel to be placed on to above hybridization hybrid chip substrate layer, be directed at corresponding hybridization subregion, adds magnetic material below chip
The bed of material, magnetic material layer attract the substrate layer containing magnetic material to reach sealing effect.
In conclusion biochip hybridizing device provided by the invention can solve in the prior art chip due to multiple subregions
The problem of causing cross contamination, can get good hybridization sealing effect.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalents made by bright specification and accompanying drawing content are applied directly or indirectly in relevant technical field, similarly include
In scope of patent protection of the invention.
Claims (6)
1. a kind of biochip hybridizing device, which is characterized in that include from the bottom to top successively magnetic material layer that layer is set, wait hybridize
Chip substrate layer, layer of silica gel and the interior substrate layer equipped with magnetic material, the no sample to hybridization hybrid chip substrate layer is facing towards described
Magnetic material layer, the point sample to hybridization hybrid chip substrate layer is facing towards the layer of silica gel；One surface of the substrate layer, which is equipped with, to be fixed
Slot, the layer of silica gel are protruded into the fixing groove by a surface of layer of silica gel, and the layer of silica gel through-thickness is partially submerged into
The fixing groove；Another surface of the layer of silica gel is equipped at least one subregion for being mutually mutually separated and sealing, and is arranged in layer of silica gel
The subregion of at least one respectively on the point sample face to hybridization hybrid chip substrate layer the detection sample of at least one correspond；
The magnetic material layer and the interior substrate layer magnetic absorption equipped with magnetic material.
2. biochip hybridizing device according to claim 1, which is characterized in that the magnetic material layer and the substrate
Layer is correspondingly arranged.
3. biochip hybridizing device according to claim 1, which is characterized in that the number for the subregion being arranged in layer of silica gel
It is 1-24.
4. biochip hybridizing device according to claim 1, which is characterized in that the material of the substrate layer is plastics.
5. biochip hybridizing device according to claim 1, which is characterized in that the substrate layer the preparation method comprises the following steps:
Plastic cement for injection molding is added into using magnetic material, injection molding prepares the substrate layer that magnetic material is equipped in described.
6. biochip hybridizing device according to claim 1, which is characterized in that a surface of the substrate layer is equipped with
At least one subregion for being mutually mutually separated and sealing, the subregion of at least one that is arranged on substrate layer respectively be arranged in layer of silica gel
The subregion of at least one is arranged in a one-to-one correspondence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811136092.0A CN109337805A (en) | 2018-09-28 | 2018-09-28 | A kind of biochip hybridizing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811136092.0A CN109337805A (en) | 2018-09-28 | 2018-09-28 | A kind of biochip hybridizing device |
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| Publication Number | Publication Date |
|---|---|
| CN109337805A true CN109337805A (en) | 2019-02-15 |
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ID=65307444
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811136092.0A Pending CN109337805A (en) | 2018-09-28 | 2018-09-28 | A kind of biochip hybridizing device |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6258593B1 (en) * | 1999-06-30 | 2001-07-10 | Agilent Technologies Inc. | Apparatus for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber |
| US20060127932A1 (en) * | 2003-06-03 | 2006-06-15 | Dirk Fischer | Method for the SNP analysis on biochips having oligonucleotide areas |
| US20160016166A1 (en) * | 2013-03-14 | 2016-01-21 | Diagnostics For All, Inc. | Molecular diagnostic devices with magnetic components |
-
2018
- 2018-09-28 CN CN201811136092.0A patent/CN109337805A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6258593B1 (en) * | 1999-06-30 | 2001-07-10 | Agilent Technologies Inc. | Apparatus for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber |
| US20060127932A1 (en) * | 2003-06-03 | 2006-06-15 | Dirk Fischer | Method for the SNP analysis on biochips having oligonucleotide areas |
| US20160016166A1 (en) * | 2013-03-14 | 2016-01-21 | Diagnostics For All, Inc. | Molecular diagnostic devices with magnetic components |
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