CN109336992A - A kind of preparation method of high beta-dextran content cell wall - Google Patents
A kind of preparation method of high beta-dextran content cell wall Download PDFInfo
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- CN109336992A CN109336992A CN201811128620.8A CN201811128620A CN109336992A CN 109336992 A CN109336992 A CN 109336992A CN 201811128620 A CN201811128620 A CN 201811128620A CN 109336992 A CN109336992 A CN 109336992A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The present invention provides a kind of method for preparing high beta-dextran cell wall product, using following process: seeing attached drawing 1.The cell wall product prepared by this method, beta-dextran content are stablized 40% or more, and existing product has huge raising more on the market.And raw material of the invention is the byproduct of yeast cells extracting, it is low in cost.The cell wall product of high beta-dextran content is widely used in feed, the fields such as food.So the present invention has great economic prospect.
Description
Technical field
The invention belongs to yeast cell wall purification technique fields, and in particular to waste yeast cell wall glucan purifies new
The mild method of type finally obtains the cell wall product of high sugar.
Background technique
Yeast cell wall is main byproduct in yeast extract production, it is estimated that 100 tons of extracts of every annual output, greatly
40 tons of waste yeast cell wall about can be obtained, and (aqueous about 80%), the generation of yeast cell wall have entire ecological environment important
It influences；Yeast cell wall nutritive value is relatively abundant, containing the nutrition such as glucan, mannosan, electrolytes and minerals at
Point.And with the continuous reinforcement for subtracting drag degree, yeast cell wall is more recognized as a kind of efficient immunopotentiator, and market holds
Amount constantly expands.But cell wall homogeneity currently on the market is more serious, dog-eat-dog.Glucan is as in yeast cell wall
Main function ingredient, content be cell wall quality important indicator and main action component, content is usually in 20-
Between 25%, if developing cell wall product of a content 40% or more, it will there is the competitiveness for greatly improving such product
With use value.
The yeast cell wall of high beta-dextran content be using waste yeast cell wall as raw material, using certain process,
By the protein degradation in yeast cell wall at amino acid and polypeptide, dissociate cell wall, and retain the glucan in cell wall,
Mannosan and other nutritional ingredients, product made of finally cell wall washing is dusted.But the domestic glucan that improves contains at present
The technique of amount is to utilize strong acid and strong base combined method, and the shortcomings that such method is obvious: 1 strong acid and strong base can destroy a part
Glucan and mannan structures, especially under aerobic conditions；2 yields are relatively low, lead to high expensive；3 high-contents are difficult to
Guarantee.General cell wall product can only apply to feedstuff industry, and nutritive value is low.4 generate a large amount of acid, alkali waste water, increase
Add expenses of environmental protection.So how yeast cell wall product quality, the application value of yeast cell wall is applied to high-end feed
Industry, the attached value of product for promoting cell wall will be a kind of developing direction and trend.
Summary of the invention
The present invention is low primarily directed to yeast cell wall utilization rate, and traditional handicraft strong acid and strong base combined techniques disadvantage is obvious, adopts
With relatively mild method, the yeast cell wall of high beta-dextran content is obtained.
A kind of preparation method of high beta-dextran content cell wall, which is characterized in that use following steps: (1) sizing mixing: by ferment
Mother cell wall is made into the concentration of 8-12%；
(2) cell wall activates: lye is added in cell wall slurry and adjusts PH to 7.5-8.5, reaction kettle is warming up to 110-125
DEG C, keep the temperature 2-4h；
(3) protease hydrolyzed and cell wall enzymatic hydrolysis are carried out: alkali protease and plant hydrolyzed-enzyme is added in the cell wall slurry of activation
It being digested, the additive amount of the alkali protease is the 1-5 ‰ of cell wall dry biomass, enzymolysis time 6-10 hours,
The additive amount of the plant hydrolyzed-enzyme is the 0.5-2 ‰ of cell wall dry biomass, and enzymolysis time acts on 5-8 hours；
(4) enzyme deactivation: 80-90 DEG C of heating keeps the temperature 30min-60min, cools down 60-65 DEG C；
(5) it separates, centrifuge centrifugation takes heavy phase；
(6) it washs；
(7) dry.
Wherein, the step (3) protease hydrolyzed and plant hydrolyzed-enzyme are successively to carry out, first using alkali protease into
Row protease hydrolyzed reuses plant hydrolyzed-enzyme and carries out cell wall enzymatic hydrolysis.The enzymatic hydrolysis environment of alkali protease is 55-65 DEG C,
PH7.0-8.0；The enzymatic hydrolysis environment of vegetation water enzymatic hydrolysis is 45-50 DEG C, PH4.5-5.5.
Wherein, the PH of lye is 8.0-9.0, preferably sodium hydroxide solution in the step (2).
Wherein, the step (1) yeast cell wall source is the byproduct in yeast extract production process.
Inventor passes through further investigation discovery, and raw material is that yeast cells living then needs self-dissolving broken wall and uses nuclease,
So the raw material that the present invention uses is the byproduct in yeast extract production process, mainly become the cell being crushed.
The beta-dextran content for the yeast cell wall that traditional enzymatic isolation method obtains is 20-40%, to find out its cause, maximum obstacle is just
It is that the structure of cell wall is not destroyed completely, so that the effect of enzymatic hydrolysis is not enough.And the structure of yeast cell wall is opposite
For it is more stable, structure is increasingly complex, thus how well destroy cell wall constituent become a problem.Due to albumen
The characteristics of effect of enzyme has its own and specificity, so the present inventor's well-chosen basonuclin enzyme, and to thin before enzymatic hydrolysis
Cell wall is pre-processed --- and cell wall activation, high temperature is handled plus special alkaline condition, so that the structure of cell wall is sent out
Certain variation is given birth to, the basonuclin enzyme for being more conducive to the later period is digested, and is substantially increased yeast cell wall glucans and is contained
Amount is stablized 40% or more.
Beneficial effect
1. improving beta-dextran content in cell wall product, prior art 20-25%, products of the present invention glucan contains
Amount is 40-50%；
2. not using the strong acid and strong base in traditional preparation methods, relatively mild cell wall activation and enzymatic isolation method are used, has been protected
The content of glucan has been demonstrate,proved, while environment will not be polluted；
3. enzyme amount decreases than before, cost is more worthwhile.
Detailed description of the invention
Fig. 1, detailed process of the present invention.
Specific embodiment
See Fig. 1, preparation method according to the present invention is described further in conjunction with the embodiments, it should explanation,
The following description is only intended to explain the invention, is not defined to its content.
Embodiment 1
Specifically comprises the processes of:
(1) size mixing: the waste material after yeast is extracted recycles, and is made into the slurry that cell wall content is 10wt%；
(2) cell wall activates: NaOH solution is added in cell wall slurry and adjusts PH to 8, is warming up to 120 DEG C using reaction kettle, protects
Warm 4h；
(3) protease hydrolyzed: the cell wall slurry of activation is cooled to 60 DEG C, PH to 7.5 is adjusted, cell wall dry matter is added
4.5 ‰ alkali proteases act on 8 hours；
(4) cell wall digests: 48 DEG C of cooling adjusts PH to 5, and ‰ plant hydrolyzed-enzyme of cell wall dry matter 2wt is added, and effect 7 is small
When；
(5) enzyme deactivation: being warming up to 90 DEG C, keeps the temperature 60min, is cooled to 65 DEG C；Separation: high-speed dish piece centrifuge, 5000r/ are used
Min, centrifugation, takes heavy phase；
(6) it washs: the heavy phase after separation being washed twice, material-water ratio=1:1；
(7) it dusts: cell wall consolidating for 12-18% of adjustment being contained, spray-dried powdering product；
(8) it packs: in make-up room by finished product packing.
Embodiment 2
(1) size mixing: the waste material after yeast is extracted recycles, and is made into the slurry that cell wall content is 8wt%；
(2) cell wall activates: NaOH solution is added in cell wall slurry and adjusts PH to 7.5, is warming up to 125 DEG C using reaction kettle,
Keep the temperature 4h；
(3) protease hydrolyzed: the cell wall slurry of activation is cooled to 65 DEG C, PH to 7 is adjusted, cell wall dry biomass is added
4 ‰ alkali proteases act on 8 hours；
(4) cell wall digests: 50 DEG C of cooling adjusts PH to 5.5, and 1.8 ‰ plant hydrolyzed-enzyme of cell wall dry matter, effect 8 is added
Hour；
(5) enzyme deactivation: being warming up to 85 DEG C, keeps the temperature 60min, is cooled to 65 DEG C；Separation: high-speed dish piece centrifuge, 5000r/ are used
Min, centrifugation, takes heavy phase；
(6) it washs: the heavy phase after separation being washed twice, material-water ratio=1:1；
(7) it dusts: consolidating for cell wall adjustment 15% being contained, spray-dried powdering product；
(8) it packs: in make-up room by finished product packing.
Embodiment 3
(1) size mixing: the waste material after yeast is extracted recycles, and is made into the slurry that cell wall content is 12wt%；
(2) cell wall activates: NaOH solution is added in cell wall slurry and adjusts PH to 8.5, is warming up to 110 DEG C using reaction kettle,
Keep the temperature 2h；
(3) protease hydrolyzed: the cell wall slurry of activation is cooled to 65 DEG C, PH to 8 is adjusted, 3 ‰ alkali of cell wall dry matter is added
Property protease, act on 10 hours；
(4) cell wall digests: 45 DEG C of cooling adjusts PH4.5, and 1.8 ‰ plant hydrolyzed-enzyme of cell wall dry matter is added, and effect 7 is small
When；
(5) enzyme deactivation: being warming up to 85 DEG C, keeps the temperature 30min, is cooled to 60 DEG C；Separation: high-speed dish piece centrifuge, 5000r/ are used
Min, centrifugation, takes heavy phase；
(6) it washs: the heavy phase after separation being washed twice, material-water ratio=1:1；
(7) it dusts: consolidating for cell wall adjustment 16% being contained, spray-dried powdering product；
(8) it packs: in make-up room by finished product packing.
Comparative example 1
Change (3) alkali protease the step of embodiment 1 into traditional protease --- papain or bacterialprotease,
His step such as embodiment 1.
Comparative example 2
Omit the cell wall activation step of embodiment 1, other steps such as embodiment 1.
Comparative example 3
By the raw material of embodiment 1 --- the waste material after yeast cells extracting is changed to yeast cells living, other steps such as embodiment
1。
Beta-dextran content statistics is carried out to the product of embodiment, as a result as follows:
It is therefore seen that the preferred basonuclin enzyme of the present invention, and pre-treatment and activation cell wall is carried out before enzymatic treatment, the two is made jointly
With, effectively recycled saccharomycete extract after waste material in cell wall, improve the content of product glucan.
Claims (5)
1. a kind of preparation method of high beta-dextran content cell wall, which is characterized in that use following steps: (1) sizing mixing: by yeast
Cell wall is made into the concentration of 8-12%；
(2) cell wall activates: lye is added in cell wall slurry and adjusts PH to 7.5-8.5, reaction kettle is warming up to 110-125
DEG C, keep the temperature 2-4h；
(3) protease hydrolyzed and cell wall enzymatic hydrolysis are carried out: alkali protease and plant hydrolyzed-enzyme is added in the cell wall slurry of activation
It being digested, the additive amount of the alkali protease is the 1-5 ‰ of cell wall dry biomass, enzymolysis time 6-10 hours,
The additive amount of the plant hydrolyzed-enzyme is the 0.5-2 ‰ of cell wall dry biomass, and enzymolysis time acts on 5-8 hours；
(4) enzyme deactivation: 80-90 DEG C of heating keeps the temperature 30min-60min, cools down 60-65 DEG C；
(5) it separates, centrifuge centrifugation takes heavy phase；
(6) it washs；
(7) dry.
2. preparation method according to claim 1, which is characterized in that step (3) protease hydrolyzed and vegetation water
Solution enzyme is successively to carry out, and first carries out protease hydrolyzed using alkali protease, reuses plant hydrolyzed-enzyme and carries out cell wall enzymatic hydrolysis.
3. preparation method according to claim 2, which is characterized in that the enzymatic hydrolysis environment of alkali protease is 55-65 DEG C,
PH7.0-8.0；The enzymatic hydrolysis environment of vegetation water enzymatic hydrolysis is 45-50 DEG C, PH4.5-5.5.
4. preparation method according to claim 1, which is characterized in that the PH of lye is 8.0- in the step (2)
9.0, preferably sodium hydroxide solution.
5. preparation method according to claim 1, which is characterized in that step (1) the yeast cell wall source is ferment
Byproduct in female extract production process.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811128620.8A CN109336992A (en) | 2018-09-27 | 2018-09-27 | A kind of preparation method of high beta-dextran content cell wall |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811128620.8A CN109336992A (en) | 2018-09-27 | 2018-09-27 | A kind of preparation method of high beta-dextran content cell wall |
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| CN109336992A true CN109336992A (en) | 2019-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811128620.8A Pending CN109336992A (en) | 2018-09-27 | 2018-09-27 | A kind of preparation method of high beta-dextran content cell wall |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101012468A (en) * | 2007-02-07 | 2007-08-08 | 天津科技大学 | Yeast glucans extraction process |
| CN101570769A (en) * | 2008-04-29 | 2009-11-04 | 安琪酵母股份有限公司 | Yeast glucan and mannan and production method thereof |
-
2018
- 2018-09-27 CN CN201811128620.8A patent/CN109336992A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101012468A (en) * | 2007-02-07 | 2007-08-08 | 天津科技大学 | Yeast glucans extraction process |
| CN101570769A (en) * | 2008-04-29 | 2009-11-04 | 安琪酵母股份有限公司 | Yeast glucan and mannan and production method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王静等: ""提取酵母细胞壁中β-D-葡聚糖的新方法"", 《食品与发酵工业》 * |
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Application publication date: 20190215 |