CN109316495A - A kind of interior method for extracting antibacterial liquid of natural plants body - Google Patents
A kind of interior method for extracting antibacterial liquid of natural plants body Download PDFInfo
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- CN109316495A CN109316495A CN201811429557.1A CN201811429557A CN109316495A CN 109316495 A CN109316495 A CN 109316495A CN 201811429557 A CN201811429557 A CN 201811429557A CN 109316495 A CN109316495 A CN 109316495A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The present invention relates to biopharmaceutical technologies, the invention discloses a kind of methods that antibacterial liquid is extracted in natural plants body, a kind of interior method for extracting antibacterial liquid of natural plants body, the making step of this method is as follows: step 1: the selection and extraction pre-treatment of natural frond, select rhizome is sturdy, fresh and tender natural frond 2-5 parts of leaf bud, then by natural plants handling be placed in pulverizer, obtain natural frond disintegrating slag, taking-up is placed in flask, spare.Natural frond disintegrating slag is dissolved as solvent by 50% ethyl alcohol, convenient for the antibacterial substance in natural frond disintegrating slag is dissolved out, the characteristic dissolved using ethyl alcohol, be conducive to improve rate of dissolution, to improve extraction efficiency, the form mixed using disintegrating slag part powder with supernatant, so that the antibacterial substance in natural plants body is maximumlly dissolved out, the purity for being conducive to improve antibacterial liquid, to improve the fungistatic effect of finished product antibacterial liquid.
Description
Technical field
The present invention relates to biopharmaceutical technology, the method for antibacterial liquid is extracted in specially a kind of natural plants body.
Background technique
Germ in daily life, at every moment in the health of infringement people, most of the time, human body is immunized
System can deal with the invasion of these germs, be more than that system of defense can be dealt with but if the unexpected mass propagation of germ
Range is successively fallen ill, and from the perspective of preventive medicine, it is critically important for inhibiting the growth of bacterium.
Bacteriostatic agent is exactly the substance that can inhibit bacterial growth, and bacteriostatic agent is able to suppress the growth of bacterium, prevents bacteria breed
Excessively, harm health, most antibacterial liquid can effectively inhibit the growth of bacterium, and bacteriostatic agent on the market is mostly artificial antibacterial
What liquid was obtained plus pharmaceutical synthesis, it is larger to human injury, it is easy to produce drug resistance, and the antibacterial substance in natural plants body is
Pure natural substance, it is smaller to human injury, but it is still immature for the extractive technique of antibacterial substance in natural plants body at present,
Extract relatively complicated, extraction efficiency is lower, for this purpose, it is proposed that a kind of method for extracting antibacterial liquid in natural plants body.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of method that antibacterial liquid is extracted in natural plants body, solutions
Artificial synthesized antibacterial liquid of having determined is larger to human injury, is easy to produce drug resistance, for antibacterial substance in natural plants body
Extractive technique is still immature, extracts relatively complicated, the lower problem of extraction efficiency.
To achieve the above object, the invention provides the following technical scheme: extracting antibacterial liquid in a kind of natural plants body
The making step of method, this method is as follows:
Step 1: the selection and extraction pre-treatment of natural frond
Select rhizome is sturdy, fresh and tender natural frond 2-5 parts of leaf bud, then by natural plants handling be placed in pulverizer, obtain
To natural frond disintegrating slag, taking-up is placed in flask, spare；
Step 2: the preliminary extraction of natural plants body fluid
50% ethyl alcohol is selected then flask to be placed on iron stand, point as solvent by 50% ethanol injection flask of 1-3 part
Alcolhol burner is fired, is heated 20-50 minutes, after standing 10-30 minutes, supernatant is extracted, takes out spare；
Step 3: the filtering and processing of disintegrating slag
Disintegrating slag in step 2 is filtered out by filter screen, is then placed it in oven heat, hot-air seasoning to 6-8 at dry, then
Obtained disintegrating slag hirudo leech are put into mortar and are ground, it is spare to obtain the taking-up of disintegrating slag powder；
Step 4: the extraction again of natural plants body fluid
The supernatant that step 2 obtains and the disintegrating slag powder that step 3 obtains are placed in beaker and mixed, 1-3 parts of clear water are added,
It impregnates 30-45 minutes, then places the beaker on iron stand, light alcolhol burner, heating is carried out to the mixed solution in beaker and is decocted
It boils, after solution to be mixed boils 5-10 minutes, extinguishes alcolhol burner and be filtered operation again after solution to be mixed is cooling, obtain
Extracting solution.
Preferably, 50% ethyl alcohol is advisable in the step 2 with just submerging natural frond disintegrating slag.
Preferably, the filter screen aperture in the step 3 is 10mm.
Preferably, this process is ground in the step 3, needs for disintegrating slag hirudo leech to be ground into the solid that diameter is 1mm
Particle.
Preferably, in the step 4, disintegrating slag powder is first poured into beaker bottom, then pours into supernatant along glass bar
Inside beaker, then glass bar is stirred, mixing liquid is stirred, stirred 3-5 minutes, disintegrating slag powder mixes with supernatant
It is even.
Preferably, the filter operation in the step 4, uses aperture to be filtered operation for the filter screen of 0.8mm.
The present invention provides a kind of method for extracting antibacterial liquid in natural plants body, have it is following the utility model has the advantages that
The method that antibacterial liquid is extracted in the natural plants body, by selection rhizome, sturdy, the fresh and tender natural frond of leaf bud, makes
Antibacterial liquid raw material it is more fresh, be conducive to improve natural plants body in antibacterial substance effective component, thus improve finished product suppression
The fungistatic effect of bacterium solution, then as solvent natural frond disintegrating slag is dissolved by 50% ethyl alcohol, convenient for by natural frond
Antibacterial substance in disintegrating slag is dissolved out, the characteristic dissolved using ethyl alcohol, is conducive to improve rate of dissolution, to improve extraction effect
Rate, the form mixed using disintegrating slag part powder with supernatant, so that the antibacterial substance in natural plants body maximumlly dissolves out
Come, is conducive to the purity for improving antibacterial liquid, to improve the fungistatic effect of finished product antibacterial liquid.
Specific embodiment
Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
The present invention provides a kind of technical solution:
A kind of interior method for extracting antibacterial liquid of natural plants body, the making step of this method are as follows:
Step 1: the selection and extraction pre-treatment of natural frond
Select rhizome is sturdy, fresh and tender natural frond 2-5 parts of leaf bud, then by natural plants handling be placed in pulverizer, obtain
To natural frond disintegrating slag, taking-up is placed in flask, spare；
Step 2: the preliminary extraction of natural plants body fluid
Select 50% ethyl alcohol as solvent, by 50% ethanol injection flask of 1-3 part, 50% ethyl alcohol is just to submerge natural frond
Disintegrating slag is advisable, and then flask is placed on iron stand, lights alcolhol burner, heats 20-50 minutes, after standing 10-30 minutes, mentions
Supernatant is taken, is taken out spare；
Step 3: the filtering and processing of disintegrating slag
Disintegrating slag in step 2 is filtered out by filter screen, filter screen aperture is 10mm, is then placed it in oven heat, hot wind
6-8 is dried at dry, then obtained disintegrating slag hirudo leech are put into mortar and are ground, it is 1mm that disintegrating slag hirudo leech, which are ground into diameter,
Solid particle, obtain disintegrating slag powder take out it is spare；
Step 4: the extraction again of natural plants body fluid
The supernatant that step 2 obtains and the disintegrating slag powder that step 3 obtains are placed in beaker and mixed, disintegrating slag powder is first fallen
Enter beaker bottom, then supernatant poured into inside beaker along glass bar, then stir glass bar, mixing liquid is stirred,
Stirring 3-5 minutes, disintegrating slag powder is uniformly mixed with supernatant, adds 1-3 parts of clear water, is impregnated 30-45 minutes, is then set beaker
In on iron stand, alcolhol burner is lighted, heating decoction is carried out to the mixed solution in beaker, solution to be mixed boils 5-10 minutes
Afterwards, extinguish alcolhol burner to be filtered operation again after solution to be mixed is cooling, aperture is used to carry out for the filter screen of 0.8mm
Filter operation, obtains extracting solution.
Embodiment 1
A kind of interior method for extracting antibacterial liquid of natural plants body, the making step of this method are as follows:
Step 1: the selection and extraction pre-treatment of natural frond
It selects that rhizome is sturdy, 2 parts of the fresh and tender natural frond of leaf bud, then natural plants handling is placed in pulverizer, is obtained
Natural frond disintegrating slag, taking-up is placed in flask, spare；
Step 2: the preliminary extraction of natural plants body fluid
Select 50% ethyl alcohol as solvent, by 1 part of 50% ethanol injection flask, 50% ethyl alcohol is broken just to submerge natural frond
Slag is advisable, and then flask is placed on iron stand, lights alcolhol burner, heats 20 minutes, stands after ten minutes, extracts supernatant
Liquid takes out spare；
Step 3: the filtering and processing of disintegrating slag
Disintegrating slag in step 2 is filtered out by filter screen, filter screen aperture is 10mm, is then placed it in oven heat, hot wind
It is dry to be dried to 6 one-tenth, then obtained disintegrating slag hirudo leech are put into mortar and are ground, it is 1mm's that disintegrating slag hirudo leech, which are ground into diameter,
It is spare to obtain the taking-up of disintegrating slag powder for solid particle；
Step 4: the extraction again of natural plants body fluid
The supernatant that step 2 obtains and the disintegrating slag powder that step 3 obtains are placed in beaker and mixed, disintegrating slag powder is first fallen
Enter beaker bottom, then supernatant poured into inside beaker along glass bar, then stir glass bar, mixing liquid is stirred,
Stirring 3 minutes, disintegrating slag powder is uniformly mixed with supernatant, adds 1 part of clear water, is impregnated 30 minutes, is then placed the beaker brandreth
On platform, alcolhol burner is lighted, heating decoction is carried out to the mixed solution in beaker, solution boiling to be mixed after five minutes, extinguishes alcohol
Lamp is filtered operation again, uses aperture to be filtered operation for the filter screen of 0.8mm, obtain after solution to be mixed is cooling
Extracting solution.
Embodiment 2
A kind of interior method for extracting antibacterial liquid of natural plants body, the making step of this method are as follows:
Step 1: the selection and extraction pre-treatment of natural frond
It selects that rhizome is sturdy, 3 parts of the fresh and tender natural frond of leaf bud, then natural plants handling is placed in pulverizer, is obtained
Natural frond disintegrating slag, taking-up is placed in flask, spare；
Step 2: the preliminary extraction of natural plants body fluid
Select 50% ethyl alcohol as solvent, by 2 part of 50% ethanol injection flask, 50% ethyl alcohol is broken just to submerge natural frond
Slag is advisable, and then flask is placed on iron stand, lights alcolhol burner, heats 35 minutes, stands after twenty minutes, extracts supernatant
Liquid takes out spare；
Step 3: the filtering and processing of disintegrating slag
Disintegrating slag in step 2 is filtered out by filter screen, filter screen aperture is 10mm, is then placed it in oven heat, hot wind
It is dry to be dried to 7 one-tenth, then obtained disintegrating slag hirudo leech are put into mortar and are ground, it is 1mm's that disintegrating slag hirudo leech, which are ground into diameter,
It is spare to obtain the taking-up of disintegrating slag powder for solid particle；
Step 4: the extraction again of natural plants body fluid
The supernatant that step 2 obtains and the disintegrating slag powder that step 3 obtains are placed in beaker and mixed, disintegrating slag powder is first fallen
Enter beaker bottom, then supernatant poured into inside beaker along glass bar, then stir glass bar, mixing liquid is stirred,
Stirring 4 minutes, disintegrating slag powder is uniformly mixed with supernatant, adds 2 parts of clear water, is impregnated 37 minutes, is then placed the beaker brandreth
On platform, alcolhol burner is lighted, heating decoction is carried out to the mixed solution in beaker, after solution to be mixed boils 8 minutes, extinguishes alcohol
Lamp is filtered operation again, uses aperture to be filtered operation for the filter screen of 0.8mm, obtain after solution to be mixed is cooling
Extracting solution.
Embodiment 3
A kind of interior method for extracting antibacterial liquid of natural plants body, the making step of this method are as follows:
Step 1: the selection and extraction pre-treatment of natural frond
It selects that rhizome is sturdy, 5 parts of the fresh and tender natural frond of leaf bud, then natural plants handling is placed in pulverizer, is obtained
Natural frond disintegrating slag, taking-up is placed in flask, spare；
Step 2: the preliminary extraction of natural plants body fluid
Select 50% ethyl alcohol as solvent, by 3 part of 50% ethanol injection flask, 50% ethyl alcohol is broken just to submerge natural frond
Slag is advisable, and then flask is placed on iron stand, lights alcolhol burner, heats 48 minutes, after standing 30 minutes, extracts supernatant
Liquid takes out spare；
Step 3: the filtering and processing of disintegrating slag
Disintegrating slag in step 2 is filtered out by filter screen, filter screen aperture is 10mm, is then placed it in oven heat, hot wind
It is dry to be dried to 8 one-tenth, then obtained disintegrating slag hirudo leech are put into mortar and are ground, it is 1mm's that disintegrating slag hirudo leech, which are ground into diameter,
It is spare to obtain the taking-up of disintegrating slag powder for solid particle；
Step 4: the extraction again of natural plants body fluid
The supernatant that step 2 obtains and the disintegrating slag powder that step 3 obtains are placed in beaker and mixed, disintegrating slag powder is first fallen
Enter beaker bottom, then supernatant poured into inside beaker along glass bar, then stir glass bar, mixing liquid is stirred,
Stirring 5 minutes, disintegrating slag powder is uniformly mixed with supernatant, adds 3 parts of clear water, is impregnated 45 minutes, is then placed the beaker brandreth
On platform, alcolhol burner is lighted, heating decoction is carried out to the mixed solution in beaker, solution boiling to be mixed after ten minutes, extinguishes wine
Smart lamp is filtered operation again, uses aperture to be filtered operation for the filter screen of 0.8mm, obtain after solution to be mixed is cooling
To extracting solution.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. extracting the method for antibacterial liquid in a kind of natural plants body, it is characterised in that: the making step of this method is as follows:
Step 1: the selection and extraction pre-treatment of natural frond
Select rhizome is sturdy, fresh and tender natural frond 2-5 parts of leaf bud, then by natural plants handling be placed in pulverizer, obtain
To natural frond disintegrating slag, taking-up is placed in flask, spare；
Step 2: the preliminary extraction of natural plants body fluid
50% ethyl alcohol is selected then flask to be placed on iron stand, point as solvent by 50% ethanol injection flask of 1-3 part
Alcolhol burner is fired, is heated 20-50 minutes, after standing 10-30 minutes, supernatant is extracted, takes out spare；
Step 3: the filtering and processing of disintegrating slag
Disintegrating slag in step 2 is filtered out by filter screen, is then placed it in oven heat, hot-air seasoning to 6-8 at dry, then
Obtained disintegrating slag hirudo leech are put into mortar and are ground, it is spare to obtain the taking-up of disintegrating slag powder；
Step 4: the extraction again of natural plants body fluid
The supernatant that step 2 obtains and the disintegrating slag powder that step 3 obtains are placed in beaker and mixed, 1-3 parts of clear water are added,
It impregnates 30-45 minutes, then places the beaker on iron stand, light alcolhol burner, heating is carried out to the mixed solution in beaker and is decocted
It boils, after solution to be mixed boils 5-10 minutes, extinguishes alcolhol burner and be filtered operation again after solution to be mixed is cooling, obtain
Extracting solution.
2. extracting the method for antibacterial liquid in a kind of natural plants body according to claim 1, it is characterised in that: the step
50% ethyl alcohol is advisable in rapid two with just submerging natural frond disintegrating slag.
3. extracting the method for antibacterial liquid in a kind of natural plants body according to claim 1, it is characterised in that: the step
Filter screen aperture in rapid three is 10mm.
4. extracting the method for antibacterial liquid in a kind of natural plants body according to claim 1, it is characterised in that: the step
This process is ground in rapid three, needs for disintegrating slag hirudo leech to be ground into the solid particle that diameter is 1mm.
5. extracting the method for antibacterial liquid in a kind of natural plants body according to claim 1, it is characterised in that: the step
In rapid four, disintegrating slag powder is first poured into beaker bottom, is then poured into supernatant inside beaker along glass bar, then stir glass
Stick is stirred mixing liquid, stirs 3-5 minutes, disintegrating slag powder is uniformly mixed with supernatant.
6. extracting the method for antibacterial liquid in a kind of natural plants body according to claim 1, it is characterised in that: the step
Filter operation in rapid four uses aperture to be filtered operation for the filter screen of 0.8mm.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583134A (en) * | 2004-06-03 | 2005-02-23 | 北京乾露春科技有限公司 | Effervescent tablets of taraxacum mongolicum and their preparation |
| CN102961412A (en) * | 2012-10-10 | 2013-03-13 | 中国科学院过程工程研究所 | Ball-milling coupling extraction integrated process for strengthening natural plant active ingredient extraction |
| CN103120727A (en) * | 2012-12-17 | 2013-05-29 | 广西梧州制药（集团）股份有限公司 | Traditional Chinese medicine composition for treating gynecological inflammation and preparation method thereof |
-
2018
- 2018-11-28 CN CN201811429557.1A patent/CN109316495A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583134A (en) * | 2004-06-03 | 2005-02-23 | 北京乾露春科技有限公司 | Effervescent tablets of taraxacum mongolicum and their preparation |
| CN102961412A (en) * | 2012-10-10 | 2013-03-13 | 中国科学院过程工程研究所 | Ball-milling coupling extraction integrated process for strengthening natural plant active ingredient extraction |
| CN103120727A (en) * | 2012-12-17 | 2013-05-29 | 广西梧州制药（集团）股份有限公司 | Traditional Chinese medicine composition for treating gynecological inflammation and preparation method thereof |
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Application publication date: 20190212 |