CN109316419B - Plant composition fermentation raw stock for balancing skin micro-ecology and preparation and application thereof - Google Patents
Plant composition fermentation raw stock for balancing skin micro-ecology and preparation and application thereof Download PDFInfo
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- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The invention discloses a plant composition fermentation protoplasm for balancing skin micro-ecology, and a preparation method and an application thereof. The plant composition fermentation protoplasm is prepared by an optimal preparation method which is obtained by performing repeated optimized fermentation extraction tests on the basis of an optimal formula of coix seed, polygonatum odoratum, centella asiatica, schizonepeta and cactus, wherein the plant composition fermentation protoplasm is reasonably compounded according to a traditional Chinese medicine dispatching principle of 'monarch, minister, assistant and guide'; the plant composition fermentation protoplasm can be used in cosmetics to effectively maintain the skin micro-ecological balance and promote the skin health; the prepared cosmetic has effects of balancing skin micro-ecology, promoting growth of skin beneficial bacteria, inhibiting activity of harmful bacteria, regulating skin oil secretion, inhibiting acne generation, and reducing skin sensitivity.
Description
Technical Field
The invention relates to the field of cosmetics, and mainly relates to plant composition fermentation protoplasm for balancing skin micro-ecology, and preparation and application thereof.
Background
The skin is a natural barrier of the human body surface, a plurality of microorganisms exist, the skin micro-ecological balance keeps the stability of the skin environment, and the skin has important effect on the health of the body.
Under the condition of normal flora balance, a large amount of beneficial bacteria on the surface of the skin play a role in protecting the health of the skin. After growing at a specific part on the skin surface, the probiotics are in an advantage in nutrition competition, change the pH value of the environment or release antibiotics through self metabolism to inhibit the growth of harmful bacteria and foreign bacteria and generate biological antagonism on other flora. The probiotics can also absorb and utilize skin excreta such as inorganic salt, uric acid, urea and the like, balance the surface of the skin, resist the invasion of heavy metal ions to cells, retain water, moisturize and the like. When the skin is disturbed, the quantity of resident bacteria and/or transient bacteria of the skin is increased sharply, which causes the disturbance of the flora of the skin, thereby causing a series of skin problems such as vigorous secretion of skin grease, skin inflammation, acne, scurf and head itch, and the like.
If the skin care product can provide a nutrient component for the skin, promote the growth of beneficial bacteria of the skin and inhibit the reproduction of harmful bacteria of the skin, the pH value of the skin can be maintained, the balance of moisture and nutrients of the skin can be maintained, the sensitivity and the inflammation can be eliminated, and the barrier of the skin can be enhanced. Currently, actives for balancing the skin micro-ecology have been the focus of research in the cosmetic field.
Chinese patent CN201710604164.9 discloses a composition with the effects of maintaining and recovering skin microecology and a preparation method thereof, and is characterized by comprising the following components of stem natural juice, fresh fruit juice, specially selected sugar and isomalt, wherein the composition has the effects of resisting inflammation and promoting the generation of antibacterial peptide, and can maintain and recover the health of the skin microecology.
The Chinese invention patent CN201310469004.X discloses a composition capable of promoting skin microecological balance and a preparation method thereof, the composition takes skin prebiotics alpha-glucan oligosaccharide, fructooligosaccharide, beta-glucan, plant extract, yeast extract and natural antibacterial agent leuconostoc/radish root fermentation product filtrate as main components, the composition fundamentally regulates the skin microecological balance, maintains the skin health, and overcomes the defects that the traditional cosmetics/skin care products are not used for treating symptoms and root causes and are easy to generate side effects.
The invention provides a safe and effective plant composition fermentation raw stock for cosmetics from the aspect of skin micro-ecological balance, which conforms to the development trend of cosmetics and has wide application prospect and great market value.
Disclosure of Invention
The invention provides a plant composition fermented protoplasm for balancing skin micro-ecology and a preparation method thereof.
In order to achieve the purpose, the following technical scheme is adopted
A plant composition protoplasm for balancing skin microecology is prepared from (by weight parts) Coicis semen 5-50, rhizoma Polygonati Odorati 5-40, herba Centellae 5-30, herba Schizonepetae 5-30, and radix et caulis Opuntiae Dillenii 5-30.
Preferably, the plant composition protoplasm (I) for balancing skin microecology is prepared from the following components, by weight, 20-40 parts of coix seeds, 20-25 parts of polygonatum odoratum, 15-20 parts of centella asiatica, 10-20 parts of schizonepeta and 10-20 parts of cactus.
Preferably, the plant composition protoplasm for balancing skin microecology is prepared from the following components, by weight, 35 parts of coix seeds, 25 parts of polygonatum odoratum, 15 parts of centella asiatica, 15 parts of schizonepeta and 10 parts of cactus.
Preferably, the plant composition protoplasm (II) for balancing skin microecology is prepared from the following components, by weight, 40 parts of coix seeds, 20 parts of polygonatum odoratum, 15 parts of centella asiatica, 10 parts of schizonepeta and 15 parts of cactus.
Preferably, the plant composition protoplasm (III) for balancing skin microecology is prepared from the following components, by weight, 30 parts of coix seeds, 20 parts of polygonatum odoratum, 15 parts of centella asiatica, 15 parts of schizonepeta and 20 parts of cactus.
Preferably, the plant composition protoplasm (IV) for balancing skin microecology is prepared from the following components, by weight, 20 parts of coix seeds, 20 parts of polygonatum odoratum, 20 parts of centella asiatica, 20 parts of schizonepeta and 20 parts of cactus.
The preparation method of the plant composition fermented protoplasm adopts a fermentation extraction method, namely, the plant composition is fermented, and then the plant composition fermented protoplasm capable of balancing skin micro-ecology is obtained by filtering, sterilizing, centrifugally separating, decoloring and filtering. The method specifically comprises the following steps:
(1) the bacteria used for fermentation is lactococcus lactis, and the bacteria used for fermentation is added in a bacteria liquid form, and the concentration of the bacteria liquid is 10 5 -10 8 cfu/mL；
(2) Preparing materials: removing impurities from the plant materials, cleaning, pulverizing to 20-200 mesh, and mixing to obtain plant composition; the preparation concentration is 10 5 -10 8 cfu/mL bacterial liquid, the pH value is adjusted to 6.5-7.5;
(3) fermentation: mixing the bacterial liquid, the plant composition and water according to the proportion, and fermenting for 40-72 hours at the temperature of 28-45 ℃ and under the condition of 50-300r/min to obtain fermentation liquor;
(4) and (3) purification: filtering the obtained fermentation broth, and autoclaving the filtrate at 105-125 deg.C for 20-40min; centrifuging the sterilized fermented filtrate at 3000-8000r/min and a centrifugation radius of 5-20cm for 10-35min, removing precipitate, collecting supernatant, decolorizing with active carbon, filtering, and collecting filtrate to obtain plant composition fermented protoplasm for balancing skin microecology.
Preferably, in step (2), the powder is pulverized to 60 mesh.
Preferably, in the step (3), fermenting for 60 hours at 30-40 ℃ and 50r/min to obtain fermentation liquor;
the dosage ratio of the bacterial liquid to the plant composition to the water is 5-30 mL:15g-60g:200-600g.
Preferably, the dosage ratio of the bacterial liquid to the plant composition to the water is 8-20 mL:25g-50g:300-500g.
Preferably, the plant composition fermented protoplasm (I) for balancing skin microecology has the dosage ratio of the bacterial liquid, the plant composition and water of 10mL:20g:300g.
Preferably, the plant composition fermented protoplasm (II) for balancing skin microecology has the dosage ratio of the bacterial liquid, the plant composition and water of 15mL:30g of: 420g.
Preferably, the plant composition fermented puree (iii) for balancing skin micro-ecology is prepared by mixing the bacterial liquid, the plant composition and water in a ratio of 12mL:25g of: 400g.
Preferably, the plant composition fermented protoplasm (IV) for balancing skin microecology has a dosage ratio of 12mL:24g:380g.
Preferably, in the step (4), the filtrate is autoclaved at the temperature of 110-115 ℃ for 30min; centrifuging the sterilized fermentation filtrate at 3000-5000r/min with a centrifugation radius of 8-12cm for 20-40min, removing the precipitate, and collecting the supernatant.
The second purpose of the invention is to apply the plant composition fermentation protoplasm to cosmetics and provide the cosmetics for balancing the skin micro-ecology.
In order to achieve the purpose of the invention, the invention adopts the following technical scheme:
the plant composition fermentation raw stock is applied to the preparation of cosmetics, and the addition amount of the plant composition fermentation raw stock in the cosmetics is 0.1-100% by mass.
The invention also provides a cosmetic with the function of balancing skin microecology, which contains the plant composition fermented protoplasm.
The cosmetic contains 0.1-100% of the plant composition fermentation raw stock in percentage by mass.
The cosmetic is as follows: aqua, emulsion, cream, facial mask, jelly, powder, spray, essence and washing and caring.
The mechanism of the invention is as follows:
the plant composition fermented raw pulp for balancing the skin microecology is obtained by taking the traditional Chinese medicine formula theory of monarch, minister, assistant and guide as guidance and carrying out a large number of experimental researches by the inventor. In the invention, in the process of long-term production research, the inventor deeply excavates effective species with functions of repairing, relieving, anti-allergy, anti-inflammation, dispelling red and relieving itching in traditional plants, reasonably compounds the effective species according to the principle of prescription development of 'monarch, minister, assistant and guide' in traditional Chinese medicine, and obtains the compound preparation by the optimal preparation method obtained through repeated optimized fermentation and extraction tests on the basis of the optimal formula of fragrant solomonseal rhizome, aloe, dwarf lilyturf tuber, bletilla striata and cactus.
In the plant formula, the coix seeds are used as monarch drugs, have sweet and light taste and are slightly cold in nature, have the effects of promoting the subsidence of swelling by water, invigorating spleen and removing dampness, relaxing muscles and tendons and removing arthralgia, clearing heat and expelling pus and the like, are rich in amino acid, coixol ester, triterpenoid, protein, carbohydrate, vitamin B1 and the like, and have the effects of removing acnes, removing freckles, improving chapped skin, pachylosis and the like. Centella asiatica is used as a ministerial drug, has the effects of detoxifying, reducing swelling, resisting bacteria and diminishing inflammation, is rich in asiaticoside, and has the effects of treating skin ulcer and promoting skin growth. The polygonatum odoratum and the schizonepeta are used as adjuvant drugs, the polygonatum odoratum has the effects of nourishing yin, moistening dryness, clearing heat, promoting fluid production, relieving cough and the like, contains rich polygonatum odoratum polysaccharide, and has a remarkable moisturizing effect; herba Schizonepetae has effects of inducing sweat, relieving exterior syndrome, dispelling toxic materials, promoting eruption, regulating blood, stopping bleeding, promoting skin blood circulation, promoting healing of skin infectious diseases, and promoting absorption of affected tissue. The cactus flavone has the effects of inhibiting tyrosinase activity, scavenging oxygen free radicals, whitening skin, beautifying and resisting aging. The traditional Chinese medicine composition has the advantages that the traditional Chinese medicine composition is combined, the medicine effects are mutually coordinated, the effects of moisturizing and nourishing skin, promoting skin wound healing, killing pathogenic bacteria and fungi and maintaining skin health can be achieved, and meanwhile, the traditional Chinese medicine composition also has certain beautifying and face nourishing effects.
Compared with the prior art, the invention has the following advantages:
(1) The plants are reasonably compounded according to the principle of 'monarch, minister, assistant and guide' prescription, can realize synergistic interaction, maximally exerts the beautifying effect of the raw materials, and has more comprehensive effect than a single plant.
(2) The preparation process provided by the invention has high extraction rate, and the fermentation extraction method has the advantages that the production process can be effectively simplified, so that the reaction process is carried out under mild conditions. Meanwhile, the fermentation extraction method can also change the molecular structure of the components of the raw materials, improve the efficacy of the raw materials, reduce toxicity and irritation, optimize the color, taste and other advantages, is an ideal novel plant extraction process, and is beneficial to popularization and application.
(3) When the plant composition fermentation protoplasm is used in cosmetics, the skin micro-ecological balance can be effectively maintained, and the skin health is promoted; the product can regulate water and oil balance of skin, and make skin smooth and tender; soothing sensitivity, enhancing skin barrier; also has certain inhibiting effect on generation and recurrence of acne.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
Example 1A plant composition puree (I) for balancing the skin micro-ecology and a method for preparing the same
A plant composition protoplasm for balancing skin microecology is prepared from the following components in parts by weight: 35 parts of coix seeds, 25 parts of polygonatum, 15 parts of centella, 15 parts of schizonepeta and 10 parts of cactus.
The preparation method comprises the following steps:
(1) the preparation concentration is 10 5 -10 8 cfu/mL bacterial liquid, the pH value is adjusted to 6.5-7.5;
(2) preparing materials: removing impurities from the plant materials, cleaning, pulverizing to 60 mesh, and mixing to obtain plant composition;
(3) and (3) fermentation: mixing the bacterial liquid, the plant composition and water according to the proportion of 10mL:20g:300g, and fermenting for 48 hours at the temperature of 30-40 ℃ and the speed of 100r/min to obtain fermentation liquor;
(4) and (3) purification: filtering the obtained fermentation liquid, and autoclaving the filtrate at 115 deg.C for 30min; centrifuging the sterilized fermentation filtrate for 25min under the conditions of 4000r/min and the centrifugal radius of 10cm, removing the precipitate, collecting the supernatant, decolorizing with active carbon, filtering, and collecting the filtrate to obtain the plant composition fermentation protoplasm (I).
Example 2A plant composition puree for balancing the micro-ecology of the skin (II)
A plant composition protoplasm for balancing skin microecology is prepared from the following plant components in parts by weight: 40 parts of coix seeds, 20 parts of polygonatum, 15 parts of centella, 10 parts of schizonepeta and 15 parts of cactus.
The preparation method comprises the following steps of (1) preparing a mixture of bacterial liquid, plant composition and water according to a ratio of 15mL:30g of: 420g of the mixture, and the rest is the same as the example 1, so as to prepare the plant composition fermented protoplasm (II) for balancing the skin micro-ecology provided by the invention.
Example 3A plant composition puree (III) for balancing the micro-ecology of the skin
A plant composition protoplasm for balancing skin microecology is prepared from the following plant components in parts by weight: 30 parts of coix seeds, 20 parts of polygonatum odoratum, 15 parts of centella asiatica, 15 parts of schizonepeta and 20 parts of cactus.
The preparation method comprises the following steps of (1) preparing a mixture of bacterial liquid, a plant composition and water according to a proportion of 12mL:25g of: 400g of the mixture "and the rest is the same as the example 1, and the plant composition fermented puree (III) for balancing the skin micro-ecology provided by the invention is prepared.
Example 4A plant composition puree for Balancing skin micro-ecology (IV)
A plant composition protoplasm for balancing skin microecology is prepared from the following plant components in parts by weight: 20 parts of coix seeds, 20 parts of polygonatum, 20 parts of centella, 20 parts of schizonepeta and 20 parts of cactus.
The preparation method comprises the following steps of (1) preparing a mixture of bacterial liquid, a plant composition and water according to a proportion of 12mL:24g:380g of mixed materials, the rest is the same as the embodiment 1, and the plant composition fermentation protoplasm (IV) for balancing skin micro-ecology provided by the invention is prepared.
Comparative examples 1-5 fermentation broths of Individual plants
Weighing 100 parts of plant coix seed, 100 parts of polygonatum, 100 parts of centella, 100 parts of schizonepeta and 100 parts of cactus respectively. The fermentation extraction was performed according to the preparation method described in example 1, and the single plant fermentation broths were prepared in sequence, respectively.
Comparative example 6 plant composition extract
The amount of the plant composition used was in accordance with example 1, i.e. the plant composition was made up of plant material components comprising the following weight percentages: 30% of polygonatum, 20% of aloe, 20% of radix ophiopogonis, 15% of bletilla striata and 15% of cactus.
The preparation method comprises the following steps: removing impurities from each plant raw material, cleaning, crushing to 60 meshes, adding deionized water according to a liquid-material mass ratio of 15.
Test example 1 skin micro-ecological balance test
1.1 preparation of the samples
The experimental medium of example 1, comparative examples 1 to 6 and a commercial glucose control medium of 0.5% were each prepared at a concentration of 0.5%.
1.2 principle of the experiment
The experiment will compare and test the absorption and utilization rate of the skin flora to the specific sample and the common glucose, thereby researching the consumption process of the specific probiotic nutrients by the skin flora.
1.3 Experimental methods
A portion of the flora on the skin surface was transplanted into the following examples, comparative examples and control media, respectively, and the amount of residual glucose/plant puree nutrients was calculated 48 hours after the test.
The measurements are shown in table 1 below.
TABLE 1 consumption metrics
| Consumption level | Consumption state |
| 0-20% | No consumption |
| 20-40% | Poor degree of consumption |
| 40-60% | Moderate degree of consumption |
| 60-100% | Up to total consumption |
1.4 results of the experiment
The results of the experiment are shown in Table 2.
TABLE 2 comparison of actual consumption
The common glucose has higher absorption and utilization rate for both pathogenic bacteria and probiotics, the plant composition fermentation protoplasm, the single plant fermentation product and the plant composition extract can be bioselectively protected and utilized by the probiotics on the skin surface, and are not obviously metabolized by the pathogenic bacteria, and the plant composition fermentation protoplasm is relatively more bioselective compared with the pathogenic bacteria which are used as biological inhibitors, wherein the test proves that other examples have similar effects.
Test example 2 competitive growth test
2.1 preparation of the samples
The test media (tryptone USP) of example 1 and comparative examples 1 to 6 were prepared at a concentration of 0.5%, respectively.
2.2 Principle of experiment
And testing the influence and the effect of the specific plant composition fermentation raw stock, the single plant fermentation raw stock and the plant composition extracting solution on the production condition of the flora when the probiotics and the pathogenic bacteria coexist.
2.3 Experimental method
The colonies were incubated in the test medium (trypsin pulsed USP) containing 0.5% of the bacteria of example 1, comparative examples 1-6, and the number of bacteria was counted after 24 hours.
Mixing a medium: micrococcus clarkii and staphylococcus aureus.
2.4 Results of the experiment
The results of the experiment are shown in Table 3.
TABLE 3 competitive test results
Experimental data show that the nutrients in the plant composition fermentation raw pulp, the single plant fermentation raw pulp and the plant composition extracting solution can promote the growth of probiotics, pathogenic bacteria can not promote the growth of the probiotics, but the quantity of the pathogenic bacteria is gradually reduced due to the lack of food and nutrition, so that the effects of being selectively utilized by the probiotics and inhibiting the pathogenic bacteria are achieved; the plant composition fermentation raw pulp of the embodiment has better effects of promoting probiotics and inhibiting pathogenic bacteria than single plant fermentation raw pulp and plant composition extracting solution, and tests prove that other embodiments have similar effects.
Test example 3 irritation evaluation test
And evaluating the irritation of the fermentation liquor by adopting a rabbit erythrocyte hemolysis experiment. The specific operation is as follows: separately, 500. Mu.L of the sample prepared in example 1 and comparative examples 1-6 was placed in a 2mL centrifuge tube, 500. Mu.L of a 2% rabbit red blood cell (commercially available) suspension was added, gently shaken and mixed, incubated in a 37 ℃ incubator for 3 hours, centrifuged at 2000r/min for 3 minutes, the supernatant was extracted, the absorbance was measured at a wavelength of 414nm using physiological saline as a negative control and distilled water as a positive control, and the hemolysis was calculated according to the formula. The results are shown in Table 4.
TABLE 4 results of hemolysis
The comparison of experimental results shows that the hemolysis ratio of the fermented protoplasm of the plant composition is much lower than that of the fermentation liquor of a single plant prepared by the proportion of 1-5, and the irritation of the fermentation liquor is obviously reduced after the composition. At the same time, the content of the plant composition is lower than that of the plant composition of the comparative example 6, which shows that the stimulating components in the plant composition are reduced after the fermentation treatment.
Application example 1 plant composition fermentation puree emulsion
A skin microecological emulsion containing plant composition fermented puree has a specific formula shown in Table 5.
TABLE 5
The preparation method of the emulsion comprises the following steps:
1. heating and dissolving an additive phase raw material p-hydroxyacetophenone in 1, 2-hexanediol in advance until the mixture is clear and transparent for later use;
2. putting the water phase into an emulsifying pot, uniformly stirring, heating and stirring to 75-85 ℃;
3. putting the oil phase into an oil phase preparation pot, uniformly stirring, and heating and stirring to 75-85 ℃;
4. pumping the materials in the oil phase pot into an emulsifying pot, uniformly stirring, vacuumizing the emulsifying pot, and homogenizing and emulsifying for 10 minutes;
5. keeping the temperature for 15 minutes, then cooling to 40 ℃ by cooling water, adding aminomethyl propanol and the plant composition fermentation raw stock (I) described in the embodiment 1, and uniformly stirring;
6. cooling to 35 ℃, and discharging after the inspection is qualified.
Application example 2 Balanced skin micro-ecological mask
A skin micro-ecological mask containing the fermented protoplasm of the plant composition has a formula shown in Table 6.
TABLE 6
| Components | Mass fraction/%) |
| Deionized water | To 100 |
| Glycerol | 10.00 |
| 1, 3-propanediol | 7.00 |
| Hyaluronic acid sodium salt | 0.10 |
| Xanthan gum | 0.10 |
| Hydroxyethyl cellulose | 0.10 |
| Allantoin | 0.50 |
| Panthenol | 1.00 |
| 1, 2-hexanediol | 0.60 |
| P-hydroxyacetophenone | 0.50 |
| The plant composition fermentation broth (I) described in example 1 | 30.00 |
The preparation method comprises the following steps:
(1) Dispersing xanthan gum and sodium hyaluronate in glycerol in advance, adding the dispersion, allantoin, panthenol and hydroxyethyl cellulose into deionized water, heating to 80 ℃, stirring and dissolving until the mixture is uniform and transparent, and cooling;
(2) Heating p-hydroxyacetophenone in 1, 3-propylene glycol and 1, 2-hexanediol in advance to dissolve until the p-hydroxyacetophenone is transparent, and adding, stirring and dissolving until the system is transparent when the temperature is reduced to 55 ℃;
(3) Cooling to 40 ℃, adding the plant composition fermentation raw stock (I) described in the embodiment 1, and uniformly stirring until the plant composition fermentation raw stock (I) is completely dissolved;
(4) And (4) checking physicochemical indexes such as color, pH, fragrance and appearance, filtering by 200 meshes after the inspection is qualified, and discharging.
Application example 3 skin micro-ecological balancing essence
The skin micro-ecological balancing essence containing the fermented protoplasm of the plant composition has a specific formula shown in table 7.
TABLE 7
The preparation method of the essence comprises the following steps:
1. heating and dissolving an additive phase raw material p-hydroxyacetophenone in 1, 2-hexanediol in advance until the mixture is clear and transparent for later use;
2. putting the water phase into deionized water, stirring uniformly, heating and stirring to 75-85 ℃;
3. keeping the temperature for 10 minutes, then cooling to 60 ℃ with cooling water, slowly adding the p-hydroxyacetophenone dissolved in the 1, 2-hexanediol in advance while stirring, and stirring until the p-hydroxyacetophenone is dissolved uniformly;
4. cooling to 40 ℃, and adding the plant composition fermentation raw stock;
5. stirring uniformly, cooling to 35 ℃, measuring the pH value, discharging by 300 meshes after the pH value is qualified, and thus obtaining the product.
Application example 4 skin-micro-ecological toner
The invention relates to a skin micro-ecological toner containing the plant composition fermentation protoplasm, and the specific formula of the toner is shown in the table 8.
TABLE 8
| Composition (I) | Mass fraction ofFraction/%) |
| EDTA-2Na | 0.02 |
| Glycerol | 5 |
| Panthenol | 0.2 |
| 1, 3-butanediol | 5 |
| Hyaluronic acid sodium salt | 0.08 |
| P-hydroxyacetophenone | 0.4 |
| Phenoxyethanol | 0.3 |
| Essence | 0.1 |
| The plant composition fermentation protoplasm | Allowance of |
The preparation method comprises the following steps:
(1) Mixing EDTA-2Na, glycerol and panthenol, stirring, heating to 80 deg.C, stirring to obtain transparent solution;
(2) Mixing the raw materials 1, 3-butanediol and sodium hyaluronate at normal temperature, uniformly stirring, adding the mixture into the solution obtained in the step (1), keeping the temperature at 80 ℃ for 10 minutes, and stirring uniformly;
(3) Cooling to 45 deg.C under stirring, adding the rest materials, and stirring;
(4) Vacuum defoaming, discharging at 38 deg.C or below, and making the material body be aqueous solution.
Application comparative example 1 emulsion containing plant composition extract
The same conditions as in application example 1 were used, except that the plant composition fermented puree (i) was changed to comparative example 6, compared to application example 1.
Use comparative example 2 facial mask containing plant composition extract
The same conditions as in application example 1 were used, except that the plant composition fermented puree (i) was changed to comparative example 6, as compared with application example 2.
Comparative application example 3 ordinary face film
The same conditions as in application example 1 were used, except that the plant composition fermented puree (i) was changed to di water, compared to application example 2.
Application Effect example 1 evaluation of acne suppression and moisturizing efficacy test
1. Preparation of the test specimens
The acne inhibition efficacy test was performed as follows using application example 1 and application comparative example 1 as samples.
2. Experimental method
Selecting 30 volunteers with obvious acne on both sides and in a new or inflammatory stage, wherein the facial skin belongs to the easily grown acne type, the age is 20-35 years, and the half face test is carried out on the volunteers in each half of men and women. The volunteers need to apply the half-face product once a day in the morning and at night, and the half-face product is continuously tried for 28 days.
Evaluation content of acne inhibition: and (3) marking the quantity of the white comedones and the black comedones of the left face and the right face of the volunteer and counting the quantity of the acnes at 2 levels. According to the inhibiting effect on the acne, a half-face experiment trial evaluation table is collected.
Evaluation of moisturizing effect: the skin moisture content and moisture loss (TEWL) test was performed on the left and right nasolabial folds of the volunteers, and the average of 3 values was taken as the data (d 0) of the moisture content of the left and right nasolabial folds and the data (d 0) of the skin moisture loss (TEWL) of the nasolabial folds. After the test was completed, the volunteers applied the example product on one side and the comparative product on the other side as required, and the moisture content data (d 28) and nasolabial sulcus skin moisture loss (TEWL) data (d 28) were again tested 28 days after use.
3. Results of the experiment
The results of the evaluation of acne suppression are shown in table 9.
TABLE 9
The results show that the effects of the application example 5 and the application comparative example 1 are obviously different, which shows that the product containing the plant composition fermentation raw stock has a good acne inhibition effect, and the acne inhibition capability of the plant composition is remarkably improved after fermentation treatment.
The moisturizing effect evaluation results are shown in table 10.
TABLE 10 moisture content test Change Rate (%)
From the results in table 10, it can be seen that the emulsion prepared in application example 1 of the present invention and the emulsion prepared in application comparative example 1 both have good moisturizing effects, but application example 1 has better moisturizing effect than application comparative example 1, which indicates that the plant composition of the present invention has the moisturizing and synergistic effects after fermentation treatment.
Application Effect example 2 human body trial evaluation test
1. The test population: 60 people with dry facial skin, red and swollen skin and spasm and itching are selected, the age is 18-28 years, half of men and half of women are randomly divided into 2 groups, and each group comprises 15 men and 15 women. The test subject is required to be healthy, cosmetics can be used according to the standard, the sense of the cosmetics is sharp, and the judgment can be accurately made after the cosmetics are used.
2. Test samples: the face masks prepared in application example 2 and comparative examples 2 to 3 were used.
3. The test method comprises the following steps: before the test subjects try the samples, the test subjects clean the face by themselves and use the face at a fixed point every day after airing. The first group of users used the mask prepared in application example 2 on the left face and the mask prepared in application comparative example 2 on the right face; the second group of users used the facial mask prepared in application example 2 on the left face and the facial mask prepared in application comparative example 3 on the right face. After one month of continuous use, the skin condition was observed before and after use. The subjects were unable to use any other cosmetic or other medication during the trial, avoid staying up all night, smoking and maintaining a light diet. And in the using process, the moisture retention, the brightness improvement, the mildness, the fineness and the elasticity of the sample are evaluated, wherein the highest score is 5, and the lowest score is 1.
4. Test results
The actual using effect of the sample is tracked and recorded, the feedback opinions of the testers are collected and are sorted and analyzed, and the statistical result is shown in a table 11.
TABLE 11 average values of human trial results
| Evaluation results | Application example 2 | Comparative application example 2 | Comparative application example 3 |
| Moisture retention | 4.4 | 3.5 | 2.7 |
| Brightness enhancement | 3.6 | 3.1 | 2.2 |
| Mildness property | 4.7 | 4.3 | 4.7 |
| Fineness of fineness | 4.1 | 3.4 | 2.9 |
| Spring effect | 4.1 | 3.2 | 2.3 |
As can be seen from the results of table 11, the application example 2 of the present invention has a good use effect, and the facial masks prepared in the application example 2 and the application comparative example 2 of the present invention have a better use effect than the application comparative example 3, indicating that both the fermented puree of the plant composition and the extract of the plant composition have the effect of improving the skin; on the other hand, the mask prepared by the application example 2 of the invention has better using effect than the mask prepared by the application comparative example 2, which shows that the plant composition has better effect after fermentation treatment.
The embodiments of the present invention have been described in detail, but the embodiments are only examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Accordingly, equivalent alterations and modifications are intended to be included within the scope of the present invention, without departing from the spirit and scope of the invention.
Claims (3)
1. The plant composition fermented raw pulp for balancing skin microecology is characterized by being prepared from the following components in parts by weight: 35 parts of coix seeds, 25 parts of polygonatum, 15 parts of centella, 15 parts of schizonepeta and 10 parts of cactus;
the preparation method comprises the following steps:
(1) the preparation concentration is 10 5 -10 8 cfu/mL bacterial liquid, the pH value is adjusted to 6.5-7.5;
(2) preparing materials: removing impurities from the plant materials, cleaning, pulverizing to 60 mesh, and mixing to obtain plant composition;
(3) fermentation: mixing the bacterial liquid, the plant composition and water according to the proportion of 10ml:20g:300g, and fermenting for 48 hours at the temperature of 30-40 ℃ and the speed of 100r/min to obtain fermentation liquor;
(4) and (3) purification: filtering the obtained fermentation liquid, and autoclaving the filtrate at 115 deg.C for 30min; centrifuging the sterilized fermentation filtrate for 25min at 4000r/min and a centrifugal radius of 10cm, removing the precipitate, collecting the supernatant, decolorizing with active carbon, filtering, and collecting the filtrate to obtain the plant composition fermented protoplasm for balancing skin microecology.
2. A cosmetic characterized by: fermented puree comprising the plant composition for balancing the skin micro-ecology according to claim 1.
3. The cosmetic according to claim 2, characterized in that: the cosmetic contains 0.1-100% of plant composition fermented raw pulp for balancing skin micro-ecology by mass fraction; the cosmetic is as follows: aqueous solutions, emulsions, creams, facial masks, gels, powders, sprays, essences, and lotions.
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| CN111419766B (en) * | 2020-04-01 | 2022-10-21 | 爱乐星丽化妆品制造（上海）有限公司 | Moisturizing emulsion using coix seed water and preparation method thereof |
| CN112716850B (en) * | 2021-01-15 | 2023-07-18 | 曾万祥 | Composition for regulating skin microecological balance and application thereof |
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