CN109295151A - A kind of tigogenin extract in sisal hemp cream produce fermentation process - Google Patents
A kind of tigogenin extract in sisal hemp cream produce fermentation process Download PDFInfo
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- CN109295151A CN109295151A CN201811281379.2A CN201811281379A CN109295151A CN 109295151 A CN109295151 A CN 109295151A CN 201811281379 A CN201811281379 A CN 201811281379A CN 109295151 A CN109295151 A CN 109295151A
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- sisal hemp
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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Abstract
The method that the invention discloses a kind of to extract high-purity tigogenin from sisal dregs, including pond ferment, filters pressing and heap ferment step.Using fermentation process of the invention, fermentation is made to be able to sufficiently, reduce the impurity content of sisal hemp cream, substantially increase the yield and purity of tigogenin；Also the effective component tigogenin in sisal hemp is utilized significantly, reduces waste.
Description
Technical field
The present invention relates to the extraction processes of tigogenin, and in particular to a kind of tigogenin extract in sisal hemp cream fermentation side
Method.
Background technique
Tigogenin (Tigogenin tigogenin) and Chinese yam saponin (Diosgenin), hecogenin is all synthesis steroid
The starting material of body hormonal medicaments, tigogenin are to extract and obtain from the waste residue of the eastern No.1 sisal hemp of production fiber.
Currently, the domestic sisal hemp cream production technology for extracting tigogenin, generally will first extract the juicing and sword that fiber generates
Numb meat slag is stored in spontaneous fermentation in fermentation vat, and about about two months wait for that insoluble sisal hemp saponin(e is formed by spontaneous fermentation
Afterwards, then by dregs of linseeds or sesame seeds water mixed liquid with pumping it is dried to the filtering of sunning ground naturally osmotic, again, obtains impure many dry numb cream.This
During method is uneven in fermentation process, be not thorough, not exclusively since the pond ferment spontaneous fermentation period is long, and fermentation is dried
Since the period is long, the dregs of linseeds or sesame seeds largely rot, and thereby result in numb cream low output and the obtained numb cream impurity that ferments is too many, tigogenin
Content is low, causes the sulfuric acid consumed in subsequent tigogenin extraction production also corresponding too many, consumed by subsequent purification procedures
Amount of alcohol is also very big, production cost is significantly increased, and also result in the wasting of resources.
Summary of the invention
In view of the above-mentioned problems of the prior art, the object of the present invention is to provide swords in a kind of new tigogenin extraction
Numb cream produces fermentation process, to improve sisal hemp cream yield, tigogenin yield and tigogenin quality.
To achieve the above object, the technical solution adopted by the present invention is that: a kind of tigogenin extract in sisal hemp cream production hair
Fermenting process includes the following steps:
(1) the sisal hemp juice of generation pond ferment: is squeezed in the sisal hemp meat slag and drawing process obtained after fresh sisal hemp leaf is reeled off raw silk from cocoons
It is pumped into fermentation vat together with the tap water of cleaning flax silk, ferments, precipitates under room temperature, be kept completely separate when sisal dregs ferment to solid-liquid
When, by fermentation vat, clear water is all taken out at the middle and upper levels, and lower layer's sisal hemp slag liquid continues to ferment, and is sent out when darkening to blackish green, liquid level
When ferment bubble tails off, next process will be entered；
(2) filters pressing: the mixing dregs of linseeds or sesame seeds that step (1) is fermented are logical to carry out filters pressing, until numb cream extruding is anhydrous；
(3) heap ferment: then the blocky sisal hemp cream moisture control that step (2) is obtained piles sisal hemp cream greatly between 40%-50%
Heap, temperature control is between 35-45 DEG C in fermentation vat, heap ferment 25-30 days, and sisal hemp cream, which ferments, to be finished.
Preferably, the above-mentioned pond ferment time is 12-18 days.
Preferably, in above-mentioned pond ferment, virgin material and old material cannot be beaten with 5 days for boundary mixed.
Preferably, sisal hemp cream moisture content should be controlled in 15-20% after heap ferment.
Compared with prior art, the beneficial effect that the present invention has is: 1, using fermentation process of the invention, making to ferment
With abundant, the impurity content of reduction sisal hemp cream substantially increases the yield and purity of tigogenin；2, it is utilized in sisal hemp significantly
Effective component tigogenin, reduce waste；3, under the premise of obtaining same tigogenin content, when shortening fermentation
Between.4, in terms of environmental protection, water pollution is further reduced.
Specific embodiment
Now in conjunction with specific embodiment, next the invention will be further elaborated.
Embodiment one
(1) sword of generation pond ferment: is squeezed in the sisal hemp meat slag and drawing process generated after fresh sisal hemp leaf is reeled off raw silk from cocoons
Numb juice, which is pumped into large pool together under room temperature, to ferment, precipitates, 50 tons of pond capacity or more, when fermentation is kept completely separate to solid-liquid
When, by fermentation vat, clear water is all taken out at the middle and upper levels, and clear water discards, and for sisal hemp, processing factory is recycled, the high solubility sisal hemp of lower layer
Slag liquid continues to ferment, and steeps when tailing off when darkening to blackish green, liquid level fermentation, will enter next process.This workshop section technology
It is required that essentially consisting in pond ferment fermentation time and the old and new's material cannot beat mixed with 5 days for boundary, two months left sides are obtained according to traditional zymotic
The right side, and the old mixing of the old and new's material, existing process section time control was at 16 days or so；
(2) the mixing dregs of linseeds or sesame seeds that step (1) ferments are squeezed into plate and frame filter press by Pulp pump and carries out filters pressing, in plate and frame filter press
Internal pressure control in 0.6MPa or less continuous feed, be depressed into filter press filtering water outlet it is substantially anhydrous until, then stop pressure
Filter starts to unload plate, and the numb cream unloaded down must form blocking, and it is anhydrous to guarantee that numb cream squeezes, and otherwise continues filters pressing.This workshop section skill
Art require essentially consist in: numb cream must be depressed into pressure-filtering process molding it is blocking, squeeze it is anhydrous until；
(3) heap ferment: then the sisal hemp cream moisture control for obtaining step (2) to 50% or so when, sisal hemp cream is then transported to heap ferment
Canopy piles raft, heap ferment temperature of shed controls between 45 DEG C, and heap ferment 25 days, sisal hemp cream fermented to 1.5 meters of heap high left and right
Finish, due to generating heat in fermentation process, sisal hemp cream moisture content is reduced to 20% or so naturally, and the fermentation of sisal hemp cream finishes, wherein sisal hemp
Saponin biology content is 6% or so；Then the sisal hemp cream fermented is crushed to 15 mesh, it is spare.
Embodiment two
(1) sword of generation pond ferment: is squeezed in the sisal hemp meat slag and drawing process generated after fresh sisal hemp leaf is reeled off raw silk from cocoons
Numb juice, which is pumped into large pool together under room temperature, to ferment, precipitates, 50 tons of pond capacity or more, when fermentation is kept completely separate to solid-liquid
When, by fermentation vat, clear water is all taken out at the middle and upper levels, and clear water discards, and for sisal hemp, processing factory is recycled, the high solubility sisal hemp of lower layer
Slag liquid continues to ferment, and steeps when tailing off when darkening to blackish green, liquid level fermentation, will enter next process.This workshop section technology
It is required that essentially consisting in pond ferment fermentation time and the old and new's material cannot beat mixed with 5 days for boundary, two months left sides are obtained according to traditional zymotic
The right side, and the old mixing of the old and new's material, existing process section time control was at 15 days or so；
(2) the mixing dregs of linseeds or sesame seeds that step (1) ferments are squeezed into plate and frame filter press by Pulp pump and carries out filters pressing, in plate and frame filter press
Internal pressure control in 0.6MPa or less continuous feed, be depressed into filter press filtering water outlet it is substantially anhydrous until, then stop pressure
Filter starts to unload plate, and the numb cream unloaded down must form blocking, and it is anhydrous to guarantee that numb cream squeezes, and otherwise continues filters pressing.This workshop section skill
Art require essentially consist in: numb cream must be depressed into pressure-filtering process molding it is blocking, squeeze it is anhydrous until；
(3) heap ferment: then the sisal hemp cream moisture control for obtaining step (2) to 50% or so when, sisal hemp cream is then transported to heap ferment
Canopy piles raft, heap ferment temperature of shed controls between 35 DEG C, and heap ferment 30 days, sisal hemp cream fermented to 1.5 meters of heap high left and right
Finish, due to generating heat in fermentation process, sisal hemp cream moisture content is reduced to 15% naturally, and the fermentation of sisal hemp cream finishes, wherein tigogenin
Biological content is 7.2% or so；Then the sisal hemp cream fermented is crushed to 15 mesh, it is spare.
According to the method for the embodiment of the present invention two, 20 batches of scale-ups, the yield guality of tigogenin product have been carried out
As a result as shown in following table one.
Table one: the yield guality result of tigogenin product
| Lot number | Yield (%) | Tigogenin content (%) | Hecogenin content (%) | Moisture content (%) | Ash (%) | Fusing point (DEG C) |
| 1401 | 7.8 | 92.60 | 3.59 | 2.73 | 0.05 | 205-208 |
| 1402 | 7.2 | 92.10 | 3.82 | 2.39 | 0.05 | 205-208 |
| 1403 | 7.4 | 92.10 | 3.65 | 2.12 | 0.05 | 205-208 |
| 1404 | 7.8 | 92.10 | 4.15 | 2.81 | 0.03 | 205-208 |
| 1405 | 7.7 | 92.80 | 4.05 | 3.02 | 0.05 | 205-208 |
| 1406 | 7.1 | 92.70 | 3.39 | 2.00 | 0.03 | 205-208 |
| 1407 | 7.5 | 92.80 | 2.68 | 1.37 | 0.04 | 205-208 |
| 1408 | 7.5 | 93.20 | 3.36 | 2.45 | 0.02 | 205-208 |
| 1409 | 7.5 | 91.20 | 3.55 | 2.00 | 0.03 | 205-208 |
| 1410 | 7.7 | 91.90 | 2.30 | 2.77 | 0.05 | 205-208 |
| 1411 | 6.9 | 92.10 | 2.76 | 2.85 | 0.05 | 205-208 |
| 1412 | 7.6 | 90.60 | 2.73 | 2.28 | 0.07 | 205-208 |
| 1413 | 7.1 | 94.10 | 2.44 | 2.91 | 0.05 | 205-208 |
| 1414 | 6.8 | 93.50 | 2.89 | 2.80 | 0.08 | 205-208 |
| 1415 | 6.7 | 91.60 | 3.06 | 2.30 | 0.06 | 205-208 |
| 1416 | 7.1 | 96.10 | 2.19 | 2.47 | 0.05 | 205-208 |
| 1417 | 7.1 | 96.60 | 1.99 | 2.43 | 0.04 | 205-208 |
| 1418 | 7.3 | 96.70 | 2.00 | 2.47 | 0.05 | 205-208 |
| 1419 | 7.2 | 96.50 | 1.90 | 2.12 | 0.04 | 205-208 |
| 1420 | 7.1 | 96.20 | 2.10 | 2.35 | 0.05 | 205-208 |
Claims (4)
1. sisal hemp cream produces fermentation process in a kind of tigogenin extraction, include the following steps:
(1) the sisal hemp juice of generation pond ferment: is squeezed in the sisal hemp meat slag and drawing process obtained after fresh sisal hemp leaf is reeled off raw silk from cocoons
It is pumped into fermentation vat together with the tap water of cleaning flax silk, ferments, precipitates under room temperature, be kept completely separate when sisal dregs ferment to solid-liquid
When, by fermentation vat, clear water is all taken out at the middle and upper levels, and lower layer's sisal hemp slag liquid continues to ferment, and is sent out when darkening to blackish green, liquid level
When ferment bubble tails off, next process will be entered；
(2) filters pressing: the mixing dregs of linseeds or sesame seeds that step (1) is fermented are logical to carry out filters pressing, until numb cream extruding is anhydrous；
(3) heap ferment: then the blocky sisal hemp cream moisture control that step (2) is obtained piles sisal hemp cream greatly between 40%-50%
Heap, temperature control is between 35-45 DEG C in fermentation vat, heap ferment 25-30 days, and sisal hemp cream, which ferments, to be finished.
2. the sisal hemp cream according to claim 1 from tigogenin extraction produces fermentation process, which is characterized in that above-mentioned pond
The ferment time is 12-18 days.
3. sisal hemp cream produces fermentation process in tigogenin extraction according to claim 1 or 2, which is characterized in that above-mentioned
In the ferment of pond, virgin material and old material cannot be beaten with 5 days for boundary mixed.
4. sisal hemp cream produces fermentation process in tigogenin extraction according to claim 3, which is characterized in that heap ferment finishes
Sisal hemp cream moisture content should be controlled in 15-20% afterwards.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1985005126A1 (en) * | 1984-05-02 | 1985-11-21 | Universidade Federal Da Paraiba | Process for hecogenin production from anaerobic fermentation of sisal juice |
| CN102212102A (en) * | 2011-03-21 | 2011-10-12 | 钟秋汉 | Method for extracting sisal hemp cream from sisal hemp slag water |
| CN102797186A (en) * | 2012-09-11 | 2012-11-28 | 钟秋汉 | Method for extracting sisal hemp pastes and short fibers from waste sisal hemp residues |
| CN103087143A (en) * | 2013-01-09 | 2013-05-08 | 广西南剑生物科技有限公司 | Method for extracting tigogenin from squeezed juice of sisal residue |
| CN105796775A (en) * | 2016-03-31 | 2016-07-27 | 天峨县平昌生态农业有限公司 | Production method for sisal hemp paste |
| CN106421203A (en) * | 2016-12-07 | 2017-02-22 | 中国农业科学院麻类研究所 | Sisal hemp-plant leaf cream production method |
| CN109232709A (en) * | 2018-10-31 | 2019-01-18 | 石门红太阳生物科技有限公司 | A method of extracting high-purity tigogenin from sisal dregs |
-
2018
- 2018-10-31 CN CN201811281379.2A patent/CN109295151A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1985005126A1 (en) * | 1984-05-02 | 1985-11-21 | Universidade Federal Da Paraiba | Process for hecogenin production from anaerobic fermentation of sisal juice |
| CN102212102A (en) * | 2011-03-21 | 2011-10-12 | 钟秋汉 | Method for extracting sisal hemp cream from sisal hemp slag water |
| CN102797186A (en) * | 2012-09-11 | 2012-11-28 | 钟秋汉 | Method for extracting sisal hemp pastes and short fibers from waste sisal hemp residues |
| CN103087143A (en) * | 2013-01-09 | 2013-05-08 | 广西南剑生物科技有限公司 | Method for extracting tigogenin from squeezed juice of sisal residue |
| CN105796775A (en) * | 2016-03-31 | 2016-07-27 | 天峨县平昌生态农业有限公司 | Production method for sisal hemp paste |
| CN106421203A (en) * | 2016-12-07 | 2017-02-22 | 中国农业科学院麻类研究所 | Sisal hemp-plant leaf cream production method |
| CN109232709A (en) * | 2018-10-31 | 2019-01-18 | 石门红太阳生物科技有限公司 | A method of extracting high-purity tigogenin from sisal dregs |
Non-Patent Citations (3)
| Title |
|---|
| YANCHAO WANG: "Biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin by a newly isolated strain from a karst area of Guilin, China", 《YANCHAO WANG》 * |
| 孙昊: "剑麻残渣皂苷元高产率菌株的筛选及特性研究", 《湖南师范大学自然科学学报》 * |
| 邓天发: "产剑麻皂苷水解酶菌株的筛选与鉴定", 《食品与药品》 * |
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