CN109295126A - A kind of lactobacillus plantarum exocellular polysaccharide and preparation method with immunoregulatory activity - Google Patents
A kind of lactobacillus plantarum exocellular polysaccharide and preparation method with immunoregulatory activity Download PDFInfo
- Publication number
- CN109295126A CN109295126A CN201811009262.9A CN201811009262A CN109295126A CN 109295126 A CN109295126 A CN 109295126A CN 201811009262 A CN201811009262 A CN 201811009262A CN 109295126 A CN109295126 A CN 109295126A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- polysaccharide
- exocellular polysaccharide
- eps3
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention belongs to the polysaccharide technical fields in microbiology, and in particular to a kind of Microbial exopolysaccharides be arrived, more particularly to a kind of lactic acid granulose and preparation method thereof with immunoregulatory activity in lactobacillus plantarum source.The preparation method, including by the lactobacillus plantarum (Lactobacillus plantarumDL7X) fermentation obtains exocellular polysaccharide, the extraction of Thick many candies, the separation of exocellular polysaccharide and purifying, obtains the exocellular polysaccharide homogeneous components with immunoregulatory activity.The present invention isolates and purifies to obtain homogeneous components EPS3 using ion exchange and molecular sieve chromatography；Inflammatory cell model, EPS3(20-100 μ g/ml are constructed with lipopolysaccharides Stimulated Macrophages RAW264.7) it can dose-dependently inhibit lipopolysaccharide-induced RAW264.7 to generate NO；And there is negative regulation effect to iNOS and TNF-α mRNA on transcriptional level, show that DL7X exocellular polysaccharide EPS3 is effective immunoregulatory activity polysaccharide, these results are that the bacterial strain is preferably applied for fermented food industry and prepares functional food and provides new selection.
Description
Technical field
The invention belongs to the polysaccharide technical fields in microbiology, and in particular to arrive a kind of Microbial exopolysaccharides, especially
It is to be related to a kind of Exopolysaccharides Produced by Lactic Acid Bacteria and preparation method thereof with immunoregulatory activity in lactobacillus plantarum source.
Background technique
Lactic acid bacteria (LAB) exocellular polysaccharide (Exopolysaccharides, EPS) is lactic acid bacteria in growth, metabolic process
The general name of the mucus or capsular polysaccharide that are secreted into outside cell wall.The originating in lactic acid bacterium of the extracellular polysaccharide of nature is very extensive, such as
Dairy products, fermented food, animal intestinal tract etc..Recent study discovery, the exocellular polysaccharide of originating in lactic acid bacterium is food-grade polysaccharide
Good source, LAB EPS have the function of important technology, can be used as thickener, stabilizer, water binding agent etc. be applied to food,
The fields such as medicine and biogenetic products, especially in fermented dairy product production application, to yogurt, cheese and most acidified milk systems
Rheology, texture, mouthfeel and the flavor of product have important influence.In addition, research shows that EPS have different physiological roles, including
Anti-oxidant, blood pressure lowering, it is antitumor, improve and intestine microenvironment and enhance human immunity.Wherein, lactobacillus is frequently as auxiliary
Help leavening in fermented food industry.Lactobacillus during the fermentation, the in-situ preparation of EPS can improve products'texture and
Mouthfeel can also assign product nutrition and health care effect.Therefore, recent domestic is directed to the exploitation of high-yield extracellular polysaccharide lactic acid bacteria
Using having carried out a large amount of research work.
Wherein, the research about the Exopolysaccharides Produced by Lactic Acid Bacteria with immunoregulatory activity receives significant attention, and LAB EPS is logical
Often by enhancing cell-mediated immune response, such as promote T/B lymphopoiesis, phagocytic activity of monocyte, release cell
Factor etc., to enhance the immune protection effect of host.But the study found that the immunocompetence difference for the EPS that different strains generate
Very big, by monosaccharide composition, molecular weight, functional group, institute be electrically charged etc., many factors are influenced, and such as research is found compared with small molecule
Amount and acidic polysaccharose often show immunostimulatory activity, and the neutral polysaccharide of larger molecular weight then shows that immunosupress is made
With.Since LAB EPS has strain specificity, the polysaccharide otherness that different strains generate is very big, and different polysaccharide has respective
Specific function, therefore the immunocompetence for studying the LAB EPS of new isolated strains is necessary, can be function lactic acid bacteria strains
Application and the exploitation etc. of functional food theoretical foundation is provided.
Lactobacillus plantarum DL7X in the present invention is to screen the plant height obtained from Sichuan traditional-family self-control pickles to produce
Exocellular polysaccharide lactic acid bacteria.The specific name of bacterial strain: lactobacillus plantarumLactobacillus plantarum, it is micro- to be preserved in China
Biological inoculum preservation administration committee common micro-organisms center, preservation place are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3,
Deposit number are as follows: CGMCC No. 13331, preservation date: on November 22nd, 2016.
Summary of the invention
The invention discloses a kind of lactobacillus plantarum exocellular polysaccharides, by lactobacillus plantarumL. plantarumDL7X bacterium
Strain is fermented and is obtained.Preferred method are as follows: by lactobacillus plantarumL. plantarumDL7X bacterial strain is in semi-synthetic chemical culture medium
(SDM) 37 °C of standing constant temperature incubations in, fermentation liquid boiling water bath inactivate 15 min, are then centrifuged for obtaining exocellular polysaccharide supernatant, so
Afterwards by trichloroacetic acid removing protein, ethanol precipitation, dialysis and etc. obtain Thick many candies, Thick many candies pass through DEAE Sepharose
The method of CL-6B ion-exchange chromatography and Sephadex G-100 gel filtration chromatography purifying to get.Preferred method are as follows: will
Lactobacillus plantarum DL7X (CGMCC No. 13331) 37 °C of 18 ~ 24 h of constant temperature stationary culture, culture solution in SDM culture medium
15 min are inactivated by boiling water bath first, is then centrifuged for removing thallus and obtains supernatant, supernatant through trichloroacetic acid method removing protein,
It is centrifuged off albumen precipitation, is then precipitated with the pre-cooled ethanol of 3-5 times of volume, obtains Thick many candies after vacuum freeze drying；It is described
Thick many candies pass through DEAE Sepharose CL-6B cation exchange chromatography, and mobile phase is 20 mM Tris-HCl (pH
7.60) and 0.2~1.0 M NaCl, flow velocity are 1.0 mL/min；Elution fraction dialysis, vacuum drying etc. obtain preliminary purification
Polysaccharide component, then with the anti-inflammatory activity of lipopolysaccharide-induced macrophage RAW264.7 evaluation each component；With above-mentioned activity
Evaluation result is foundation, and the strongest component of selection activity carries out Sephadex G-100 sieve chromatography, and mobile phase is ultrapure water,
Flow velocity is 0.25 mL/min；Elution fraction is dialysed 3 days through the bag filter that molecular cut off is 8000 ~ 14000 Da, dialysis retention
Liquid through vacuum freeze drying to get.
The bacterial strain carries out constant temperature stationary culture in the SDM culture medium of 1 L, and Thick many candies yield can reach 362.5 mg/L.
Thick many candies after culture are through DEAE Sepharose CL-6B ion-exchange chromatography and Sephadex G-100 sieve chromatography
Method obtains a kind of exopolysaccharide component EPS3 with acid ion group after purification, and molecular weight is 2.02 × 104
Da.External activity experiment shows in the inflammatory cell model constructed with lipopolysaccharides Stimulated Macrophages RAW264.7, uses
Griess Kit detects the NO that cell generates under inflammatory conditions, its anti-inflammatory activity of Preliminary Identification；Secondly, further being detected with PCR
Inhibiting effect of the EPS3 to inflammatory factor iNOS and TNF-α mRNA.Lactobacillus plantarum DL7X institute of the present invention is extracellular as the result is shown
Polysaccharide component EPS3 can significantly inhibit the expression of NO generation and iNOS and TNF-α mRNA in lipopolysaccharide-induced macrophage,
As immunosuppressor, there is wide application value in terms of anti-inflammatory drug and correlation function food development.
The anti-inflammatory activity test of macrophages in vitro inflammatory model shows lactobacillus plantarum exocellular polysaccharide EPS3 of the invention
Relevant inflammatory factors (iNOS and TNF-α) mRNA expression is generated and inhibited with NO in lipopolysaccharide-induced macrophage is significantly inhibited
Activity.The macrophage is 264.7 cell of source of mouse macrophage RAW.
Here is the activity test in vitro and result of polysaccharide of the present invention:
Inflammatory cell model is constructed with lipopolysaccharides Stimulated Macrophages RAW264.7, evaluates the anti-inflammatory activity of exocellular polysaccharide.
With RPMI-1640 culture medium (37 °C, 5% CO for containing 10% fetal calf serum2) culture mouse macrophage RAW 264.7 and right
It is grouped.Blank control group: it is handled without LPS and polysaccharide intervention is also not added；Model control group: the LPS that 0.5 μ g/mL is added is dry
In advance；Experimental group: the lactobacillus plantarum exocellular polysaccharide component EPS3(final concentration of the LPS of 0.5 μ g/mL and various concentration difference 25,
50,100 μ g/mL) it is jointly processed by.
NO secretion level measurement: using Griess method to NO2The quantitative NO of detection indirectly is generated.Cell inoculation is trained in 96 holes
Plate is supported, for group of cells after different disposal factor acts on 24 h, every hole takes 50 μ L of cell culture fluid, with Griess reagent test,
Light absorption value is surveyed under 550 nm of microplate reader.According to NaNO2Standard curve obtained calculates the production quantity of NO in culture medium.As a result see attached
Fig. 2.
Lactobacillus plantarum exocellular polysaccharide EPS3 is in 25 ~ 100 μ g/ml measures ranges to macrophage vigor in statistics
On do not influence significantly.Therefore the 100 μ g/ml of maximum dose that this experiment is chosen is safe for macrophage.By scheming
2 as can be seen that the amount that macrophage generates NO is in foundation level under normal condition.When being stimulated by EPS, NO generates aobvious
Work increase (compared with normal group,P< 0.01).Various concentration EPS3 is incubated for the suppression of 264.7 macrophage post dose dependence of RAW
The NO secretion of LPS induction processed, when the NO secretion for the LPS induction for being able to suppress about 40% when EPS3 concentration is 100 μ g/ml.
The measurement of mRNA expression of cytokines level: it uses real time fluorescent quantitative reverse transcriptional PCR (RT-PCR)
The iNOS that technology analysis lactobacillus plantarum exocellular polysaccharide EPS3 induces LPS, the influence of IL-6 mRNA expression take exponential growth
Phase cell is added in 24 porocyte culture plates, and adjustment concentration of cell suspension is 2.5 × 105Cells/well sets 37 °C, 5%
CO224 h of incubator culture, every hole is added the EPS3 of 25,50,100 μ g/ml and is incubated for 2 h later, and LPS, which is then added, makes it
Final concentration of 0.5 μ g/ml sets 37 °C and continues to cultivate 24 h.Extract cell total rna with Trizol reagent, reverse transcription at cDNA,
Using specific primer, expanded with materials such as corresponding primer, cDNA by step on fluorescence quantitative PCR instrument, using 2−ΔΔCt
Relative quantification method carries out gene expression analysis.
Influence of the EPS3 to the mRNA expression of cytokines in LPS inducing macrophage: as shown in figure 3, in normal condition
Under, iNOS, the expression of TNF-α mRNA are seldom, and pass through after LPS stimulation, and expression quantity significantly improves.And plant cream bar
Bacterium exopolysaccharide EPS3 has apparent inhibition to iNOS mRNA expression, and has concentration dependent.EPS3 is in 100 μ g/ml
To the inhibiting rate about 50% of iNOS mRNA expression when dosage.In addition, the expression of TNF-α mRNA is also presented to a certain degree in EPS3
Inhibition, but and do not have concentration dependent, inhibitory effect compared to iNOS mRNA it is also worse, inhibit in 100 μ g/ml dosage
Rate is 28.6%.It is many studies have shown that in macrophage, NO is mainly catalyzed at inducible nitric oxide synthase (iNOS)
Lower generation, highly expressed iNOS and the NO excessively generated take part in the generation and development of diseases associated with inflammation.This research is more with rouge
264.7 macrophage of RAW of sugar stimulation is inflammatory cell model, inquires into what lactobacillus plantarum exocellular polysaccharide EPS3 activated LPS
The release of macrophage NO.EPS3 dose-dependently inhibits lipopolysaccharides in 25-100 μ g/ml dosage range as the result is shown
The macrophage of induction generates NO.Further study show that EPS3 has negative sense tune to iNOS and TNF-α mRNA on transcriptional level
Section effect.
Detailed description of the invention
Fig. 1 is that lactobacillus plantarum DL7X exists in DEAE Sepharose CL-6B anion-exchange column and EPS-3
Elution curve on Sephadex G-100 gel filtration chromatography.
Fig. 2 is that EPS3 inhibits lipopolysaccharide-induced macrophage to generate NO.
Fig. 3 is the expression that EPS3 inhibits macrophage iNOS and TNF-α mRNA.
Specific embodiment
It is prepared by the fermentation of the lactobacillus plantarum DL7X exocellular polysaccharide EPS3 with immunoregulatory activity
1, lactobacillus plantarum exocellular polysaccharide DL7X fermented and cultured in 2 L fermentation flasks
(1) culture of seed liquor
Bacterial strain needed for experiment is crossed from the glycerol tube of -80 °C of preservations to MRS plate and is activated, is placed in 37 °C of incubators
After cultivating 24 h, 5 ml MRS fluid nutrient mediums are dropped down into oese picking single bacterium, is placed in 37 °C of incubators and cultivates 16 h
Afterwards, 50 ml MRS fluid nutrient mediums are forwarded to, are placed in 37 °C of culture mediums after cultivating 16 h, it is spare.
(2) preparation of fermentation medium
It prepares the semi-synthetic chemical culture medium (SDM) of 2 L: being improved on MRS culture basis, wherein using yeast basic nitrogen source
(yeast nitrogen base, 5 g/L), peptone (bacto casitone, 10 g/L) substitute in MRS culture medium prescription
Beef extract extract, yeast extract, peptone, other components and content are consistent, cold in 121 °C of high pressure sterilization 15 min
But it is spare to be placed on room temperature.
(3) SDM culture medium fermented and cultured
16 h inoculums in MRS fluid nutrient medium are seeded in 2 L SDM culture mediums with 3% ~ 5% inoculum concentration (v/v), are set
37 °C of 24 h of culture in constant incubator.
2, the extraction of lactobacillus plantarum crude extracellular polysaccharide
2 L fermentation liquid boiling water baths are heated into 15 min, inactivate remaining thallus.The fermentation liquid of 2 L inactivation thallus is taken out later cold
But room temperature is arrived, 4 °C of 12000 × g are centrifuged 20 min removal precipitating.The trichlorine that mass fraction is 80% is added into supernatant
Acetic acid is put 4 °C of refrigerators and is stood overnight, 4 °C of 12000 × g are centrifuged 20 min and collect supernatant to final concentration of mass fraction 4%
Liquid.95% ethyl alcohol of 3~5 times of volumes pre-cooling is added in supernatant, sufficiently vibrates, polysaccharide is precipitated in flocculent deposit, 4 °C of refrigerators
It stands overnight.After polysaccharide is sufficiently precipitated in flocculent deposit, 4 °C of 12000 × g are centrifuged 20 min and collect polysaccharide precipitation.It will
Polysaccharide precipitation puts 4 °C of 3 d of refrigerator deionized water dialysis with being transferred in bag filter after hot water dissolving, and 4 h of interval are changed once
Water.Dry white puff Thick many candies are obtained after polysaccharide solution is carried out vacuum freeze drying after the completion of dialysis.
3, the preparation of the extracellular refined polysaccharide of lactobacillus plantarum
DEAE-Sepharose CL-6B ion mucilage binding is entered in chromatographic column (cm of D2.6 × 30), with Tris-HCl eluent (20
MM, pH 7.60) balance chromatographic column.Loading after Thick many candies Tris-HCl buffer solution, sample concentration are 10 mg/mL, on
70 mg of sample successively uses Tris-HCl eluent and the eluent gradient containing 0.2,0.4,0.6,0.8,1.0 mol/L NaCl
Elution, flow velocity: 2.5 mL/min, every pipe collected volume: 5 mL.Phend-sulphuric acid detects polyoses content in collecting pipe, merges and receives
Collect peak component, obtains polysaccharide sample through dialysis, concentration, freeze-drying.The result is shown in Figure 1 A, as seen from the figure, lactobacillus plantarum DL7X
Exocellular polysaccharide is mainly made of four acidic polysaccharose components.Then to be lived using immunological regulation Activity evaluation as foundation
The strongest polysaccharide component EPS-3 of property.
Component obtained above is distinguished again further enterprising in Sephadex G-100 gel column (cm of D1.0 × 100)
Row separation.Eluent: ultrapure water；Sample concentration: 10 mg/mL, applied sample amount: 20 ~ 25 mg.Flow velocity: 0.20 mL/min, every pipe
Collected volume: 3.0 mL.Phend-sulphuric acid detects polyoses content in collecting pipe, merges and collects each peak component, through dialysis, concentration,
Freeze-drying obtains polysaccharide sample.The result is shown in Figure 1 B, as seen from the figure, in DEAE Sepharose CL-6B anion-exchange column
The salt that NaCl is eluted wash component EPS-3 further pass through Sephadex G-100 gel filtration chromatography purified to obtain it is single,
Symmetrical eluting peak illustrates that lactobacillus plantarum exocellular polysaccharide EPS3 is uniform monosaccharide component, this group of lease making detection is free of albumen.
Dialysis freeze-drying: selective retention molecular weight is the bag filter of 8000-14000 Da, and above-mentioned molecular sieve is added and affords
Collection liquid, dialyse 3 days, obtain purified polysaccharide EPS3 after vacuum freeze drying, weigh to mark and simultaneously saves.Through physicochemical property point
Analysis, molecular weight are 2.02 × 104Da, polyoses content 85.36%, moisture content 10.41% are free of protein and phosphate radical.
Above example is only that embodiments of the present invention are described, and not limits the scope of the present invention
It is fixed, for those skilled in the art, above description can be improved or be deformed, but all these improve or become
Shape should all fall into the protection scope that claim of the invention determines.
Claims (3)
1. a kind of lactobacillus plantarum exocellular polysaccharide with immunoregulatory activity, by lactobacillus plantarum (L. plantarum)
DL7X(CGMCC No. 13331) strain fermentation and obtain.
2. the preparation method for the lactobacillus plantarum exocellular polysaccharide that right 1 requires, comprising: by the lactobacillus plantarum bacterium of claim 1
Strain 37 °C of standing constant temperature incubations, fermentation liquid boiling water bath in semi-synthetic chemical culture medium (SDM) inactivate 15 min, and centrifugation obtains
Exocellular polysaccharide supernatant, then by trichloroacetic acid removing protein, ethanol precipitation, dialysis and etc. obtain Thick many candies, Thick many candies warp
Cross the method purifying of DEAE Sepharose CL-6B ion-exchange chromatography and Sephadex G-100 gel filtration chromatography to get.
3. the preparation method of claim 2, comprising: by lactobacillus plantarum DL7X(CGMCC No. 13331) in SDM culture medium
37 °C of 18 ~ 24 h of constant temperature stationary culture, culture solution inactivate 15 min by boiling water bath first, are then centrifuged for removing thallus acquisition
Supernatant, supernatant are centrifuged off albumen precipitation through trichloroacetic acid method removing protein, then heavy with the pre-cooled ethanol of 3 ~ 5 times of volumes
It forms sediment, obtains Thick many candies after vacuum freeze drying；The Thick many candies are pure by DEAE Sepharose CL-6B ion-exchange chromatography
Change, mobile phase is 20 mM Tris-HCl (pH 7.60) and 0.2 ~ 1.0 M NaCl, and flow velocity is 1.0 mL/min；Elution fraction
Dialysis, vacuum drying etc. obtain the solid of preliminary purification, then evaluate respectively with lipopolysaccharide-induced macrophage RAW 264.7
The anti-inflammatory activity of component；Using above-mentioned Activity evaluation as foundation, the strongest component EPS-3 of selection activity carries out Sephadex
G-100 sieve chromatography, mobile phase are ultrapure water, and flow velocity is 0.25 mL/min；Elution fraction through molecular cut off be 8000 ~
The bag filter of 14000 Da is dialysed 3 days, and trapped fluid of dialysing is through vacuum freeze drying to get uniform more with immunoregulatory activity
Saccharic composition EPS3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811009262.9A CN109295126B (en) | 2018-08-31 | 2018-08-31 | Lactobacillus plantarum exopolysaccharide with immunoregulatory activity and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811009262.9A CN109295126B (en) | 2018-08-31 | 2018-08-31 | Lactobacillus plantarum exopolysaccharide with immunoregulatory activity and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109295126A true CN109295126A (en) | 2019-02-01 |
| CN109295126B CN109295126B (en) | 2021-01-08 |
Family
ID=65165662
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811009262.9A Active CN109295126B (en) | 2018-08-31 | 2018-08-31 | Lactobacillus plantarum exopolysaccharide with immunoregulatory activity and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109295126B (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110106121A (en) * | 2019-05-30 | 2019-08-09 | 江南大学 | A kind of lactobacillus plantarum of extracellular polysaccharide |
| CN110714038A (en) * | 2019-09-17 | 2020-01-21 | 韩美琪 | Lactobacillus plantarum exopolysaccharide and application thereof in preparation of ACE inhibitor composition |
| CN111053789A (en) * | 2019-11-26 | 2020-04-24 | 湖南营养树生物科技有限公司 | Methods and compositions for modulating the immune system |
| CN111808910A (en) * | 2020-07-22 | 2020-10-23 | 南京农业大学 | Method for evaluating activity of dietary polysaccharide |
| CN112167341A (en) * | 2020-09-27 | 2021-01-05 | 吉林农业大学 | Preparation method of dairy product containing lactobacillus plantarum JLAU103 exopolysaccharide |
| CN112458128A (en) * | 2020-12-04 | 2021-03-09 | 中国科学院海洋研究所 | Application of marine halomonas extracellular polysaccharide in preparation of immunopotentiator |
| CN112852902A (en) * | 2021-01-19 | 2021-05-28 | 南昌大学 | Enterococcus extracellular polysaccharide with immunoregulation effect and preparation method and application thereof |
| CN113373084A (en) * | 2021-05-21 | 2021-09-10 | 上海交通大学 | Antibacterial and exopolysaccharide-producing lactobacillus curvatus and application thereof |
| CN113698503A (en) * | 2021-08-16 | 2021-11-26 | 华南理工大学 | Lactobacillus plantarum neutral exopolysaccharide and application thereof |
| CN113881604A (en) * | 2021-11-10 | 2022-01-04 | 深圳大学 | Lactobacillus plantarum MM89 and polysaccharide and application thereof |
| CN114276958A (en) * | 2021-12-20 | 2022-04-05 | 无锡弘焕微生态科技有限公司 | Preparation method and application of triple probiotic fermented compound with anti-inflammatory effect |
| CN114561436A (en) * | 2022-02-28 | 2022-05-31 | 西北农林科技大学 | Preparation method and application of lactobacillus plantarum exopolysaccharide |
| CN117143781A (en) * | 2023-10-27 | 2023-12-01 | 东北农业大学 | Lactobacillus plantarum, mix metaplasia and preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011007922A1 (en) * | 2009-07-14 | 2011-01-20 | 씨제이 제일제당(주) | Novel lactobacillus plantarum and composition containing same |
| CN102453689A (en) * | 2011-12-06 | 2012-05-16 | 光明乳业股份有限公司 | Lactobacillus plantarum strain producing extracellular polysaccharide, and application thereof |
| CN103757070A (en) * | 2014-01-23 | 2014-04-30 | 光明乳业股份有限公司 | Extracellular polysaccharide with immunomodulatory effect and preparation method and application thereof |
| CN104498402A (en) * | 2014-12-19 | 2015-04-08 | 北京工商大学 | Physiologically active lactobacillus plantarum for producing exopolysaccharide and application thereof |
| CN105400725A (en) * | 2015-12-18 | 2016-03-16 | 上海理工大学 | Lactobacillus plantarum strain, and applications thereof |
| CN105441357A (en) * | 2015-12-16 | 2016-03-30 | 北京工商大学 | Lactobacillus plantarum for producing antitumor activity exopolysaccharides |
-
2018
- 2018-08-31 CN CN201811009262.9A patent/CN109295126B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011007922A1 (en) * | 2009-07-14 | 2011-01-20 | 씨제이 제일제당(주) | Novel lactobacillus plantarum and composition containing same |
| CN102453689A (en) * | 2011-12-06 | 2012-05-16 | 光明乳业股份有限公司 | Lactobacillus plantarum strain producing extracellular polysaccharide, and application thereof |
| CN103757070A (en) * | 2014-01-23 | 2014-04-30 | 光明乳业股份有限公司 | Extracellular polysaccharide with immunomodulatory effect and preparation method and application thereof |
| CN104498402A (en) * | 2014-12-19 | 2015-04-08 | 北京工商大学 | Physiologically active lactobacillus plantarum for producing exopolysaccharide and application thereof |
| CN105441357A (en) * | 2015-12-16 | 2016-03-30 | 北京工商大学 | Lactobacillus plantarum for producing antitumor activity exopolysaccharides |
| CN105400725A (en) * | 2015-12-18 | 2016-03-16 | 上海理工大学 | Lactobacillus plantarum strain, and applications thereof |
Non-Patent Citations (4)
| Title |
|---|
| JI WANG 等: ""Characterization and immunomodulatory activity of an exopolysaccharide produced by Lactobacillus plantarum JLK0142 isolated from fermented dairy tofu"", 《INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES》 * |
| KANG ZHOU 等: ""Production, purification and structural study of an exopolysaccharide from Lactobacillus plantarum BC-25"", 《CARBOHYDR POLYM》 * |
| KIYOUNG KIM 等: ""Exopolysaccharide From Lactobacillus Plantarum LRCC5310 Offers Protection Against Rotavirus-Induced Diarrhea and Regulates Inflammatory Response"", 《J DAIRY SCI》 * |
| 梁增澜 等: ""乳酸菌胞外多糖免疫活性的研究进展"", 《食品与发酵工业》 * |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110106121A (en) * | 2019-05-30 | 2019-08-09 | 江南大学 | A kind of lactobacillus plantarum of extracellular polysaccharide |
| CN110714038A (en) * | 2019-09-17 | 2020-01-21 | 韩美琪 | Lactobacillus plantarum exopolysaccharide and application thereof in preparation of ACE inhibitor composition |
| CN111053789A (en) * | 2019-11-26 | 2020-04-24 | 湖南营养树生物科技有限公司 | Methods and compositions for modulating the immune system |
| CN111808910A (en) * | 2020-07-22 | 2020-10-23 | 南京农业大学 | Method for evaluating activity of dietary polysaccharide |
| CN112167341A (en) * | 2020-09-27 | 2021-01-05 | 吉林农业大学 | Preparation method of dairy product containing lactobacillus plantarum JLAU103 exopolysaccharide |
| CN112458128B (en) * | 2020-12-04 | 2022-07-19 | 中国科学院海洋研究所 | Application of marine halomonas extracellular polysaccharide in preparation of immunopotentiator |
| CN112458128A (en) * | 2020-12-04 | 2021-03-09 | 中国科学院海洋研究所 | Application of marine halomonas extracellular polysaccharide in preparation of immunopotentiator |
| CN112852902A (en) * | 2021-01-19 | 2021-05-28 | 南昌大学 | Enterococcus extracellular polysaccharide with immunoregulation effect and preparation method and application thereof |
| CN112852902B (en) * | 2021-01-19 | 2022-09-16 | 南昌大学 | Enterococcus extracellular polysaccharide with immunoregulation effect and preparation method and application thereof |
| CN113373084A (en) * | 2021-05-21 | 2021-09-10 | 上海交通大学 | Antibacterial and exopolysaccharide-producing lactobacillus curvatus and application thereof |
| CN113698503A (en) * | 2021-08-16 | 2021-11-26 | 华南理工大学 | Lactobacillus plantarum neutral exopolysaccharide and application thereof |
| CN113698503B (en) * | 2021-08-16 | 2022-05-24 | 华南理工大学 | Lactobacillus plantarum neutral exopolysaccharide and application thereof |
| CN113881604A (en) * | 2021-11-10 | 2022-01-04 | 深圳大学 | Lactobacillus plantarum MM89 and polysaccharide and application thereof |
| CN113881604B (en) * | 2021-11-10 | 2023-02-28 | 深圳大学 | Lactobacillus plantarum MM89 and polysaccharide and application thereof |
| CN114276958A (en) * | 2021-12-20 | 2022-04-05 | 无锡弘焕微生态科技有限公司 | Preparation method and application of triple probiotic fermented compound with anti-inflammatory effect |
| CN114561436A (en) * | 2022-02-28 | 2022-05-31 | 西北农林科技大学 | Preparation method and application of lactobacillus plantarum exopolysaccharide |
| CN117143781A (en) * | 2023-10-27 | 2023-12-01 | 东北农业大学 | Lactobacillus plantarum, mix metaplasia and preparation method and application thereof |
| CN117143781B (en) * | 2023-10-27 | 2024-01-30 | 东北农业大学 | Lactobacillus plantarum, mix metaplasia and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109295126B (en) | 2021-01-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109295126A (en) | A kind of lactobacillus plantarum exocellular polysaccharide and preparation method with immunoregulatory activity | |
| CN111154676A (en) | Lactobacillus rhamnosus exopolysaccharide, preparation method thereof and bacteria used thereby | |
| CN110106119B (en) | Lactobacillus rhamnosus M9 separated from breast milk and application thereof | |
| CN111925961B (en) | Lactobacillus plantarum Lp2 and application thereof | |
| CN111560330B (en) | Lactobacillus casei with immunoregulation, anti-inflammatory and anti-cervical cancer effects and application thereof | |
| CN105441357A (en) | Lactobacillus plantarum for producing antitumor activity exopolysaccharides | |
| CN110055199A (en) | One UA149 plants of lactobacillus plantarum and its application | |
| CN113337430B (en) | Lactobacillus paracasei NSL0201 and application thereof | |
| CN104479043B (en) | A kind of exocellular polysaccharide of Lactobacillus rhamnosus and its preparation method and application | |
| Mu et al. | Probiotic yeast BR14 ameliorates DSS-induced colitis by restoring the gut barrier and adjusting the intestinal microbiota | |
| CN112430553A (en) | Lactobacillus reuteri SF-L-25 with helicobacter pylori inhibiting function and application thereof | |
| CN110484460A (en) | One plant has the lactobacillus plantarum for reducing blood fat function and its application | |
| CN109497558A (en) | Kuh-seng dregs of a decoction polysaccharide synbiotic and the preparation method and application thereof | |
| WO2023098678A1 (en) | High-protein saccharomyces cerevisiae and application thereof | |
| CN105147715B (en) | A kind of new application of Paecilomyces hepiali chen neutrality exocellular polysaccharide | |
| CN103757070B (en) | A kind of exocellular polysaccharide with immunoregulation effect and its preparation method and application | |
| CN113651898A (en) | Coprinus comatus mycelium polysaccharide and preparation method and application thereof | |
| CN101530455A (en) | Method for extracting antioxidant active substance in tasteless preserved soybean | |
| CN113151067A (en) | Extraction and separation process of lactobacillus fermentation extract | |
| CN105147719B (en) | A kind of preparation of Yupingfeng dregs of a decoction polysaccharide symphysis unit and its application in immunological regulation | |
| CN111567807A (en) | Rhamnose-containing lactobacillus composition for inhibiting inflammatory reaction and resisting vaginitis and application thereof | |
| CN113861303B (en) | Extracellular polysaccharide separated from Lactobacillus delbrueckii and Streptococcus thermophilus fermented yogurt and application thereof | |
| CN106754475A (en) | The preparation of lactic acid bacteria WXT002 and exocellular polysaccharide and function | |
| CN104531567A (en) | Lactobacillus paracasei with acid resistantance and high cholesterol clearance rate and application of lactobacillus paracasei | |
| CN105779338B (en) | A kind of ice cream butter oil substitute and method using the preparation of streptococcus thermophilus fermentation whey |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |