CN109294913A - A kind of membrane material fixed card buckle suitable for tissue culture plate - Google Patents
A kind of membrane material fixed card buckle suitable for tissue culture plate Download PDFInfo
- Publication number
- CN109294913A CN109294913A CN201811265919.8A CN201811265919A CN109294913A CN 109294913 A CN109294913 A CN 109294913A CN 201811265919 A CN201811265919 A CN 201811265919A CN 109294913 A CN109294913 A CN 109294913A
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- CN
- China
- Prior art keywords
- snap ring
- membrane material
- fixed card
- card buckle
- material fixed
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
- C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of membrane material fixed card buckle suitable for tissue culture plate, it is made of snap ring and snap ring pedestal, the snap ring pedestal is " convex shape " structure and opens top-down through-hole, the snap ring covers the top in the snap ring pedestal and squeezes engagement, connection type is interference fit connection, the outer diameter of the membrane material fixed card buckle is arranged according to the culture hole aperture of different model tissue culture plate, less than the culture hole aperture of the tissue culture plate of corresponding model.It solves the problems, such as that membrane material fold and membrane material are fitted closely with culture hole bottom, avoids cell and penetrate into film bottom, cause cell not the problem of growth causes the failure of an experiment on film；It will be trimmed after membrane material clamping, the culture hole for being then placed in tissue culture plate is operating process of the invention, simple and convenient；Finally, apparatus of the present invention are polytetrafluoroethylene (PTFE) material, have many advantages, such as that high temperature resistant, low temperature, anti-chemical reagent burn into do not adhere to any substance, cell culture will not be had an impact.
Description
Technical field
The present invention relates to biological cell assays fixture fields more particularly to a kind of membrane material suitable for tissue culture plate are solid
Fixed buckle.
Background technique
With the development of medical material technology, various membrane materials are developed to be applied to life medicine field.It was researching and developing
Cheng Zhong, carrying out assessment by properties of the cell experiment to membrane material is common, required experimental method.In cell experiment
In, the performances such as biocompatibility, cytotoxicity of membrane material are detected, in addition, cell culture is carried out on film, so that experiment
Person can sufficiently study the influence that membrane material grows cell.
During carrying out membrane material cell culture, membrane material how to be allowed to be fitted in the culture hole bottom of tissue culture plate
The most common problem, at present experimenter when encountering this problem, common settling mode be using membrane material solution or
Film is sticked to orifice plate bottom by person's culture solution, but the method is still difficult to ensure that membrane material is not in fold, and operates ten
Divide difficulty；Method also is to be lived membrane pressure using the heavy ring of suitable size, and the method disadvantage is: the material of weight ring, if not
Become rusty steel, polyvinyl chloride (PVC) etc., may grow and have an impact to cell, causes other experimental error, and rely on gravity by film
It is depressed into bottom defective tightness, cell still easily penetrates into film bottom at this time, and cell is caused not grow on film.
In conclusion being carried out in membrane material cell culture actual application using tissue culture plate, in equipment and behaviour
There is also biggish problem during work, a kind of tool easy to operate matched with tissue culture plate is researched and developed in design to seem especially
It is important.
Summary of the invention
The purpose of the present invention is to provide a kind of membrane material fixed card buckles suitable for tissue culture plate, to solve using
Tissue culture plate, which carries out membrane material in membrane material cell cultivation process, can not be fitted in the culture hole bottom of tissue culture plate, cell
Film bottom is easily penetrated into, leads to problems such as cell grow not on film and complicated for operation causes the failure of an experiment.
In order to achieve the above objectives, the technical scheme of the present invention is realized as follows:
The present invention provides a kind of membrane material fixed card buckle suitable for tissue culture plate, and the membrane material fixed card buckle is by institute
Snap ring and snap ring pedestal composition are stated, the snap ring pedestal is " convex shape " structure and opens top-down through-hole, the membrane material
The outer diameter of fixed card buckle is arranged according to the culture hole aperture of different model tissue culture plate, the outer diameter of the membrane material fixed card buckle
Less than the culture hole aperture of the tissue culture plate of corresponding model, it is ensured that the membrane material fixed card buckle can be placed in corresponding model culture
In the culture hole of plate.
Further, the snap ring covers the top in the snap ring pedestal and squeezes engagement, and connection type is interference
It is cooperatively connected.
Further, the size design of the membrane material fixed card buckle are as follows:
It (1) is 30mm~34.5mm, snap ring pedestal top for the outer diameter of the membrane material fixed card buckle of 6 porocyte culture plates
Outer diameter is 25mm, and snap ring internal diameter is 24.9mm~25mm；
It (2) is 19mm~21mm, snap ring pedestal top for the outer diameter of the membrane material fixed card buckle of 12 porocyte culture plates
Outer diameter be 18mm, snap ring internal diameter be 17.9mm~18mm；
It (3) is 13mm~15mm, snap ring pedestal top for the outer diameter of the membrane material fixed card buckle of 24 porocyte culture plates
Outer diameter be 12mm, snap ring internal diameter be 11.9mm~12mm；
It (4) is 8mm~9.5mm, snap ring pedestal top for the outer diameter of the membrane material fixed card buckle of 48 porocyte culture plates
Outer diameter be 7.5mm, snap ring internal diameter be 7.4mm~7.5mm；
It (5) is 3.5mm~4mm, snap ring pedestal top for the outer diameter of the membrane material fixed card buckle of 96 porocyte culture plates
Outer diameter be 3mm, snap ring internal diameter be 2.9mm~3mm.
Remaining size of inventive film material fixed card buckle is outer dimension of the invention, is not the present invention for manufacturing
Necessary sized can be defined according to this field rudimentary knowledge.
Further, the membrane material fixed card buckle be complete setting, the quantity of every suit membrane material fixed card buckle with it is right
Answer the culture hole number of the tissue culture plate of model consistent.
Further, the material of the snap ring and snap ring pedestal is polytetrafluoroethylene (PTFE).
The utility model has the advantages that the membrane material fixed card buckle provided by the invention suitable for tissue culture plate, in the prior art
Allow membrane material to be fitted in the culture hole bottom difficulty of tissue culture plate also or causes membrane material fold, operation difficulty big, no greatly
Enough closely to cause cell infiltration film bottom that cell is led to problems such as not grow on film, creative designing is thin with different model
The matched membrane material fixed card buckle of born of the same parents' culture plate, membrane material are fixed on inventive article, are placed in the culture hole of tissue culture plate
In, the snap ring and the clamping of snap ring pedestal can solve membrane material fold and membrane material and ask with what culture hole bottom fitted closely
Topic avoids cell and penetrates into film bottom, causes cell not the problem of growth causes the failure of an experiment on film；After membrane material clamping
Trimming, the culture hole for being then placed in tissue culture plate is operating process of the invention, simple and convenient；Finally, apparatus of the present invention
For polytetrafluoroethylene (PTFE) material, high temperature resistant, low temperature meet the requirement of experiment mesohigh steam sterilizing and absolute zero refrigeration, also have
There is the advantages of anti-chemical reagent burn into does not adhere to any substance, easy to clean, nonhazardous simultaneously has physiological inertia, will not be to cell
Culture has an impact.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for the membrane material fixed card buckle that the present invention is suitable for tissue culture plate；
Fig. 2 a is the scale diagrams for the membrane material fixed card buckle snap ring that the present invention is suitable for 24 porocyte culture plates；
Fig. 2 b is the scale diagrams for the membrane material fixed card buckle snap ring pedestal that the present invention is suitable for 24 porocyte culture plates.
In figure: 1, snap ring, 2, snap ring pedestal.
Specific embodiment
It elaborates below to the embodiment of the present invention, the present embodiment carries out under the premise of the technical scheme of the present invention
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
Refering to fig. 1, a kind of structural schematic diagram of the membrane material fixed card buckle suitable for tissue culture plate of the present invention is shown,
Membrane material fixed card buckle of the invention is made of snap ring 1 and snap ring pedestal 2, and snap ring 1 is cyclic structure, and snap ring pedestal 2 is " convex
Font " structure simultaneously opens top-down through-hole, 1 set of the snap ring the snap ring pedestal 2 top and squeeze engagement, connection side
Formula is interference fit connection, forms apparatus of the present invention membrane material fixed card buckle.
A kind of membrane material fixed card buckle suitable for tissue culture plate of the present invention is for cell culture or cell experiment
The fixation of membrane material is placed in the culture hole of tissue culture plate with tissue culture plate with the use of corollary apparatus is constituted, described
The outer diameter of membrane material fixed card buckle is less than the culture hole aperture of the tissue culture plate of corresponding model, it is ensured that the membrane material fixing card
Button can be placed in the culture hole of corresponding model culture plate.
In existing tissue culture plate, the specification of the tissue culture plate generally used are as follows:
6 well culture plate apertures: 34.8mm；12 well culture plate apertures: 22.1mm；24 well culture plate apertures: 15.6mm；48 holes
Culture plate aperture: 10.2mm；96 well culture plate apertures: 4.5mm.
The size design of inventive film material fixed card buckle are as follows:
It (1) is 30mm~34.5mm for the outer diameter of the membrane material fixed card buckle of 6 porocyte culture plates, on snap ring pedestal 2
Portion's outer diameter is 25mm, and 1 internal diameter of snap ring is 24.9mm~25mm；
It (2) is 19mm~21mm, 2 top of snap ring pedestal for the outer diameter of the membrane material fixed card buckle of 12 porocyte culture plates
Outer diameter be 18mm, 1 internal diameter of snap ring be 17.9mm~18mm；
It (3) is 13mm~15mm, 2 top of snap ring pedestal for the outer diameter of the membrane material fixed card buckle of 24 porocyte culture plates
Outer diameter be 12mm, 1 internal diameter of snap ring be 11.9mm~12mm；
It (4) is 8mm~9.5mm, 2 top of snap ring pedestal for the outer diameter of the membrane material fixed card buckle of 48 porocyte culture plates
Outer diameter be 7.5mm, 1 internal diameter of snap ring be 7.4mm~7.5mm；
It (5) is 3.5mm~4mm, 2 top of snap ring pedestal for the outer diameter of the membrane material fixed card buckle of 96 porocyte culture plates
Outer diameter be 3mm, 1 internal diameter of snap ring be 2.9mm~3mm.
Remaining size of inventive film material fixed card buckle is outer dimension of the invention, is not the present invention for manufacturing
Necessary sized can be defined according to this field rudimentary knowledge.
The size design of inventive film material fixed card buckle considers the culture hole aperture ruler of corresponding model tissue culture plate
Whether very little and membrane material fixed card buckle be placed and taken out facilitates, for common 6 hole to 96 porocyte culture plates, it is ensured that this hair
The realization of bright beneficial effect in actual application.
The membrane material fixed card buckle is complete setting, and the quantity of every suit membrane material fixed card buckle is thin with corresponding model
The culture hole number of born of the same parents' culture plate is consistent, and a set of 6 porocyte culture plates are equipped with 6 inventive film material fixed card buckles, together
Sample, a set of 12 porocyte culture plates are equipped with 12 inventive film material fixed card buckles, and so on, different model it is thin
Born of the same parents' culture plate is equipped with the corresponding inventive film material fixed card buckle, to meet the requirement of experiment.
Refering to Fig. 2 a and Fig. 2 b, the size for showing the membrane material fixed card buckle snap ring suitable for 24 porocyte culture plates is shown
It is intended to, the necessary sized except the present invention mentioned above for manufacture has also carried out the design of outer dimension.
The basic principle of inventive film material fixed card buckle are as follows: 1 set of the snap ring the snap ring pedestal 2 top and squeeze
Pressure engagement, connection type are interference fit connection, realize membrane material squeeze it is fixed described snap ring 1 and the snap ring pedestal 2 it
Between.
A kind of use process of inventive film material fixed card buckle are as follows: by 1 He of snap ring of inventive film material fixed card buckle
Snap ring pedestal 2 separates, and taking out needs the membrane material to be used for cell culture, and membrane material is covered on snap ring pedestal 2, will
Fold leveling, then clutching membrane material prevents caused membrane material deformation when placing of snap ring 1, by 1 set of snap ring on snap ring pedestal 2
The fixation to membrane material is completed in clamping at this time, and the membrane material outside snap ring 1 is trimmed, and is inverted membrane material fixed card buckle and is put into
It is used in the culture hole of tissue culture plate wait test.
It is worth noting that the material of inventive film material fixed card buckle is polytetrafluoroethylene (PTFE), polytetrafluoroethylene (PTFE) material is resistance to
High temperature, low temperature meet the requirement of experiment mesohigh steam sterilizing and absolute zero refrigeration, also have anti-chemical reagent burn into not
The advantages of adhering to any substance, easy to clean, when carrying out cell culture experiments, optimization operating process facilitates processing, and poly- four
The present invention of vinyl fluoride material manufacture, nonhazardous simultaneously have physiological inertia, will not have an impact to cell culture.
The Applicant declares that the present invention is explained by the above embodiments detail parameters and process for using of the invention, but this
Invention is not limited to above-mentioned detailed detail parameters and process for using, that is, it is above-mentioned detailed not mean that the present invention must rely on
Parameter and process for using could be implemented.It should be clear to those skilled in the art, any improvement in the present invention, to this
The equivalence replacement of each raw material of invention product and addition, the selection of concrete mode of auxiliary element etc., all fall within protection of the invention
Within range and the open scope.
Claims (5)
1. a kind of membrane material fixed card buckle suitable for tissue culture plate, which is characterized in that the membrane material fixed card buckle is by institute
Snap ring and snap ring pedestal composition are stated, the snap ring pedestal is " convex shape " structure and opens top-down through-hole, the membrane material
The outer diameter of fixed card buckle is arranged according to the culture hole aperture of different model tissue culture plate, less than the tissue culture plate of corresponding model
Culture hole aperture.
2. the membrane material fixed card buckle according to claim 1 suitable for tissue culture plate, it is characterised in that: the membrane material
Expect the outer diameter design of fixed card buckle are as follows:
It (1) is 30mm~34.5mm, snap ring pedestal top outer diameter for the outer diameter of the membrane material fixed card buckle of 6 porocyte culture plates
For 25mm, snap ring internal diameter is 24.9mm~25mm；
(2) be 19mm~21mm for the outer diameter of the membrane material fixed card buckle of 12 porocyte culture plates, snap ring pedestal top it is outer
Diameter is 18mm, and snap ring internal diameter is 17.9mm~18mm；
(3) be 13mm~15mm for the outer diameter of the membrane material fixed card buckle of 24 porocyte culture plates, snap ring pedestal top it is outer
Diameter is 12mm, and snap ring internal diameter is 11.9mm~12mm；
(4) be 8mm~9.5mm for the outer diameter of the membrane material fixed card buckle of 48 porocyte culture plates, snap ring pedestal top it is outer
Diameter is 7.5mm, and snap ring internal diameter is 7.4mm~7.5mm；
(5) be 3.5mm~4mm for the outer diameter of the membrane material fixed card buckle of 96 porocyte culture plates, snap ring pedestal top it is outer
Diameter is 3mm, and snap ring internal diameter is 2.9mm~3mm.
3. the membrane material fixed card buckle according to claim 1 suitable for tissue culture plate, it is characterised in that: the snap ring
It covers the top in the snap ring pedestal and squeezes engagement, connection type is interference fit connection.
4. the membrane material fixed card buckle according to claim 1 suitable for tissue culture plate, it is characterised in that: the membrane material
Material fixed card buckle is complete setting, the quantity of every suit membrane material fixed card buckle and the culture hole of the tissue culture plate of corresponding model
Quantity is consistent.
5. the membrane material fixed card buckle according to claim 1 suitable for tissue culture plate, it is characterised in that: the snap ring
Material with snap ring pedestal is polytetrafluoroethylene (PTFE).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811265919.8A CN109294913A (en) | 2018-10-29 | 2018-10-29 | A kind of membrane material fixed card buckle suitable for tissue culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811265919.8A CN109294913A (en) | 2018-10-29 | 2018-10-29 | A kind of membrane material fixed card buckle suitable for tissue culture plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109294913A true CN109294913A (en) | 2019-02-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811265919.8A Pending CN109294913A (en) | 2018-10-29 | 2018-10-29 | A kind of membrane material fixed card buckle suitable for tissue culture plate |
Country Status (1)
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| CN (1) | CN109294913A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013164594A (en) * | 2013-02-27 | 2013-08-22 | Oji Holdings Corp | Method for manufacturing fine rugged sheet |
| CN103270149A (en) * | 2010-10-08 | 2013-08-28 | 莱萃恩维斯柯凡股份有限公司 | Cell culture insert |
| CN205347445U (en) * | 2016-01-22 | 2016-06-29 | 天津卫凯生物工程有限公司 | Cell culture fibre membrane support frame, cell culture support and cell culture device |
| CN206885737U (en) * | 2017-06-28 | 2018-01-16 | 吴莎莎 | A kind of food containers with waterproof ventilated membrane |
-
2018
- 2018-10-29 CN CN201811265919.8A patent/CN109294913A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270149A (en) * | 2010-10-08 | 2013-08-28 | 莱萃恩维斯柯凡股份有限公司 | Cell culture insert |
| JP2013164594A (en) * | 2013-02-27 | 2013-08-22 | Oji Holdings Corp | Method for manufacturing fine rugged sheet |
| CN205347445U (en) * | 2016-01-22 | 2016-06-29 | 天津卫凯生物工程有限公司 | Cell culture fibre membrane support frame, cell culture support and cell culture device |
| CN206885737U (en) * | 2017-06-28 | 2018-01-16 | 吴莎莎 | A kind of food containers with waterproof ventilated membrane |
Non-Patent Citations (2)
| Title |
|---|
| 王笑竹等: "《机械设计》", 31 May 2017, 北京理工大学出版社 * |
| 郭云良等: "《医学生物学技术与原理》", 30 June 2009, 中国海洋大学出版社 * |
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Legal Events
| Date | Code | Title | Description |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yang Siyu Inventor after: Xu Shanshan Inventor after: Xu Haiyang Inventor before: Zhang Yujian Inventor before: Xu Shanshan Inventor before: Xu Haiyang |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190201 |